Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 5,935,346 G→C 7.2% A123P (GCG→CCG)  fau → 5‑formyltetrahydrofolate cyclo‑ligase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029475,935,3460GC 7.2% 218.6 / 6.0 69A123P (GCG→CCG) fau5‑formyltetrahydrofolate cyclo‑ligase
Reads supporting (aligned to +/- strand):  ref base G (31/33);  new base C (2/3);  total (33/36)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.32e-01

CGGCAAGTGCATCTACCTGCCGGTGCTGCATGCCTGGCCGCGCACGCGCATGGTGTTCCAGCGTTTTGAGCAGGGTGAGAAGCTCAAGCCCAACCGCTTCCGTATT‑CCAGAGCCGATTATCGATCGCAAACGCCAGCGGCC‑GATCTGGGCGCTGGATCTGATTCTGCTACCGCTGGTCGGGTTTGACGAA‑GTGGGCGGCCGCCTGGGTATGGGCGGTGGCTTCTATGATCGCAGCCTGGCCTACCAGGCGCGTCGGCAAACCTGGAAAAAACCCTTGCTGCTGG  >  NC_002947/5935198‑5935481
                                                                                                                                                      |                                                                                                                                        
cgGCAAGTGCATCTACCTGCCGGTGCTGCATGCCTGGCCGCGCACGCGCATGGTGTTCCAGCGTTTTGAGCAGGGTGAGAAGCTCAAGCCCAACCGCTTCCGTATT‑CCAGAGCCGATTATCGATCGCAAACGCCAGCGGCC‑GATCTggg                                                                                                                                          >  6:238354/1‑149 (MQ=255)
      gTGCATCTACCTGCCGGTGCTGCATGCCTGGCCGCGCACGCGCATGGTGTTCCAGCGTTTTGAGCAGGGTGAGAAGCTCAAGCCCAACCGCTTCCGTATT‑CCAGAGCCGATTATCGATCGCAAACGCCAGCGGCC‑GATCTGGGCGCTg                                                                                                                                     >  1:202193/1‑148 (MQ=255)
         cATCTACCTGCCGGTGCTGCATGCCTGGCCGCGCACGCGCATGGTGTTCCAGCGTTTTGAGCAGGGTGAGAAGCTCAAGCCCAACCGCTTCCGTATT‑CCAGAGCCGATTATCGATCGCAAACGCCAGCGGCC‑GATCTGGGCGCTGGAt                                                                                                                                  <  5:87794/148‑1 (MQ=255)
         cATCTACCTGCCGGTGCTGCATGCCTGGCCGCGCACGCGCATGGTGTTCCAGCGTTTTGAGCAGGGTGAGAAGCTCAAGCCCAACCGCTTCCGTATT‑CCAGAGCCGATTATCGATCGCAAACGCCAGCGGCC‑GATCTGGGCGCTGGAt                                                                                                                                  <  3:156554/148‑1 (MQ=255)
          aTCTACCTGCCGGTGCTGCATGCCTGGCCGCGCACGCGCATGGTGTTCCAGCGTTTTGAGCAGGGTGAGAAGCTCAAGCCCAACCGCTTCCGTATT‑CCAGAGCCGATTATCGATCGCAAACGCCAGCGCCCAGATC‑GGCCGCTggc                                                                                                                                   >  5:256686/1‑145 (MQ=255)
          aTCTACCTGCCGGTGCTGCATGCCTGGCCGCGCACGCGCATGGTGTTCCAGCGTTTTGAGCAGGGTGAGAAGCTCAAGCCCAACCGCTTCCGTATT‑CCAGAGCCGATTATCGATCGCAAACGCCAGCGCCCAGATC‑GGCCGCTggc                                                                                                                                   <  6:256686/146‑2 (MQ=255)
              aCCTGCCGGTGCTGCATGCCTGGCCGCGCACGCGCATGGTGTTCCAGCGTTTTGAGCAGGGTGAGAAGCTCAAGCCCAACCGCTTCCGTATT‑CCAGAGCCGATTATCGATCGCAAACGCCAGCGCCCAGATC‑GGCCGCTggcgt                                                                                                                                 <  1:128662/144‑4 (MQ=255)
              aCCTGCCGGTGCTGCATGCCTGGCCGCGCACGCGCATGGTGTTCCAGCGTTTTGAGCAGGGTGAGAAGCTCAAGCCCAACCGCTTCCGTATT‑CCAGAGCCGATTATCGATCGCAAACGCCAGCGCCCAGATC‑GGCCGCTggcgt                                                                                                                                 >  2:128662/1‑141 (MQ=255)
               ccTGCCGGTGCTGCATGCCTGGCCGCGCACGCGCATGGTGTTCCAGCGTTTTGAGCAGGGTGAGAAGCTCAAGCCCAACCGCTTCCGTATT‑CCAGAGCCGATTATCGATCGCAAACGCCAGCGGCC‑GATCTGGGCGCTGGATCTGa                                                                                                                              <  5:238354/146‑1 (MQ=255)
               ccTGCCGGTGCTGCATGCCTGGCCGCGCACGCGCATGGTGTTCCAGCGTTTTGAGCAGGGTGAGAAGCTCAAGCCCAACCGCTTCCGTATT‑CCAGAGCCGATTATCGATCGCAAACGCCAGCGGCC‑GATCTGGGCGCTGGATCTGa                                                                                                                              <  2:83994/146‑1 (MQ=255)
               ccTGCCGGTGCTGCATGCCTGGCCGCGCACGCGCATGGTGTTCCAGCGTTTTGAGCAGGGTGAGAAGCTCAAGCCCAACCGCTTCCGTATT‑CCAGAGCCGATTATCGATCGCAAACGCCAGCGGCC‑GATCTGGGCGCTGGATCTGATTc                                                                                                                           >  3:100872/1‑149 (MQ=255)
               ccTGCCGGTGCTGCATGCCTGGCCGCGCACGCGCATGGTGTTCCAGCGTTTTGAGCAGGGTGAGAAGCTCAAGCCCAACCGCTTCCGTATT‑CCAGAGCCGATTATCGATCGCAAACGCCAGCGCCCAGATC‑GGCCGCTggcgtttg                                                                                                                              <  2:286426/146‑7 (MQ=255)
                         ctgcATGCCTGGCCGCGCACGCGCATGGTGTTCCAGCGTTTTGAGCAGGGTGAGAAGCTCAAGCCCAACCGCTTCCGTATT‑CCAGAGCCGATTATCGATCGCAAACGCCAGCGGCC‑GATCTGGGCGCTGGATCTGa                                                                                                                              <  4:323801/136‑1 (MQ=255)
                         ctgcATGCCTGGCCGCGCACGCGCATGGTGTTCCAGCGTTTTGAGCAGGGTGAGAAGCTCAAGCCCAACCGCTTCCGTATT‑CCAGAGCCGATTATCGATCGCAAACGCCAGCGGCC‑GATCTGGGCGCTGGATCTGa                                                                                                                              >  3:323801/1‑136 (MQ=255)
                         ctgcATGCCTGGCCGCGCACGCGCATGGTGTTCCAGCGTTTTGAGCAGGGTGAGAAGCTCAAGCCCAACCGCTTCCGTATT‑CCAGAGCCGATTATCGATCGCAAACGCCAGCGGCC‑GATCTGGGCGCTGGATCTGATTCTGCTACCGc                                                                                                                  <  3:15615/148‑1 (MQ=255)
                           gcATGCCTGGCCGCGCACGCGCATGGTGTTCCAGCGTTTTGAGCAGGGTGAGAAGCTCAAGCCCAACCGCTTCCGTATT‑CCAGAGCCGATTATCGATCGCAAACGCCAGCGGCC‑GATCTGGGCGCTGGATCTGa                                                                                                                              >  4:140099/1‑134 (MQ=255)
                           gcATGCCTGGCCGCGCACGCGCATGGTGTTCCAGCGTTTTGAGCAGGGTGAGAAGCTCAAGCCCAACCGCTTCCGTATT‑CCAGAGCCGATTATCGATCGCAAACGCCAGCGGCC‑GATCTGGGCGCTGGATCTGa                                                                                                                              <  3:140099/134‑1 (MQ=255)
                             aTGCCTGGCCGCGCACGCGCATGGTGTTCCAGCGTTTTGAGCAGGGTGAGAAGCTCAAGCCCAACCGCTTCCGTATT‑CCAGAGCCGATTATCGATCGCAAACGCCAGCGGCC‑GATCTGGGCGCTGGATCTGATTCTGCTACCGCTGGTc                                                                                                             <  8:140153/149‑1 (MQ=255)
                               gCCTGGCCGCGCACGCGCATGGTGTTCCAGCGTTTTGAGCAGGGTGAGAAGCTCAAGCCCAACCGCTTCCGTATT‑CCAGAGCCGATTATCGATCGCAAACGCCAGCGGCC‑GATCTGGGCGCTGGAt                                                                                                                                  >  4:39803/1‑126 (MQ=255)
                               gCCTGGCCGCGCACGCGCATGGTGTTCCAGCGTTTTGAGCAGGGTGAGAAGCTCAAGCCCAACCGCTTCCGTATT‑CCAGAGCCGATTATCGATCGCAAACGCCAGCGGCC‑GATCTGGGCGCTGGAt                                                                                                                                  <  3:39803/126‑1 (MQ=255)
                                 cTGGCCGCGCACGCGCATGGTGTTCCAGCGTTTTGAGCAGGGTGAGAAGCTCAAGCCCAACCGCTTCCGTATT‑CCAGAGCCGATTATCGATCGCAAACGCCAGCGGCC‑GATCTGGGCGCTGGATCTGATTCTGCTACCGCTGGTCGGGt                                                                                                         >  2:106356/1‑149 (MQ=255)
                                  tGGCCGCGCACGCGCATGGTGTTCCAGCGTTTTGAGCAGGGTGAGAAGCTCAAGCCCAACCGCTTCCGTATT‑CCAGAGCCGATTATCGATCGCAAACGCCAGCGGCC‑GATCTGGGCGCTGGATCTGATTCTGCTACCGCTGGTCGGGtt                                                                                                        >  6:70602/1‑149 (MQ=255)
                                   ggCCGCGCACGCGCATGGTGTTCCAGCGTTTTGAGCAGGGTGAGAAGCTCAAGCCCAACCGCTTCCGTATT‑CCAGAGCCGATTATCGATCGCAAACGCCAGCGGCC‑GATCTGGGCGCTGGATCTGATTCTGCTACCGCTGGTCGGGttt                                                                                                       >  6:20542/1‑149 (MQ=255)
                                    gCCGCGCACGCGCATGGTGTTCCAGCGTTTTGAGCAGGGTGAGAAGCTCAAGCCCAACCGCTTCCGTATT‑CCAGAGCCGATTATCGATCGCAAACGCCAGCGGCC‑GATCTGGGCGCTGGATCTGATTCTGCTACCGCTGGTCGGGttt                                                                                                       <  4:7562/148‑1 (MQ=255)
                                     ccGCGCACGCGCATGGTGTTACAGCGTTTTGAGCAGGGTGAGAAGCTCAAGCCCAACCGCTTCCGTATT‑CCAGAGCCGATTATCGATCGCAAACGCCAGCGGCC‑GATCTGGGCGCTGGATCTGATTCTGCTACCGCTGGTCGGGTTTGa                                                                                                     <  4:100872/149‑1 (MQ=255)
                                      cgcgcacgcgctTGGTGTTCCAGCGTTTTTAGATGGGTGAGACGCTCAAGCCCACCCCTTTACGTTTTCCCCGTTTCGATTATCGATCGCAAACGCCAGCGGCC‑GATCTGGGCGCTGGATCTGATTCTGCTACCGCTGGTCGGGTTTGa                                                                                                     <  1:282842/149‑1 (MQ=255)
                                         gcacgcgcaTGGTGTTCCAGCGTTTTGAGCAGGGTGAGAAGCTCAAGCCCAACCGCTTCCGTATT‑CCAGAGCCGATTATCGATCGCAAACGCCAGCGGCC‑GATCTGGGCGCTGGATCTGATTCTGCTACCGCTGGTCGGGTTTGACGaa                                                                                                 <  7:1711/149‑1 (MQ=255)
                                                         ccAGCGTTTTGAGCAGGGTGAGAAGCTCAAGCCCAACCGCTTCCGTATT‑CCAGAGCCGATTATCGATCGCAAACGCCAGCGGCC‑GATCTGGGCGCt                                                                                                                                      <  4:167635/96‑1 (MQ=255)
                                                          cAGCGTTTTGAGCAGGGTGAGAAGCTCAAGCCCAACCGCTTCCGTATT‑CCAGAGCCGATTATCGATCGCAAACGCCAGCGGCC‑GATCTGGGCGCTGGATCTGATTCTGCTACCGCTgg                                                                                                               >  8:242033/1‑118 (MQ=255)
                                                          cAGCGTTTTGAGCAGGGTGAGAAGCTCAAGCCCAACCGCTTCCGTATT‑CCAGAGCCGATTATCGATCGCAAACGCCAGCGGCC‑GATCTGGGCGCTGGATCTGATTCTGCTACCGCTgg                                                                                                               <  7:242033/118‑1 (MQ=255)
                                                          cAGCGTTTTGAGCAGGGTGAGAAGCTCAAGCCCAACCGCTTCCGTATT‑CCAGAGCCGATTATCGATCGCAAACGCCAGCGGCC‑GATCTGGGCGCTGGATCTGATTCTGCTACCGCTGGTCGGGTTTGACGAA‑GTGGGCGGCCGCCTggg                                                                               >  4:65803/1‑149 (MQ=255)
                                                          cAGCGTTTTGAGCAGGGTGAGAAGCTCAAGCCCAACCGCTTCCGTATT‑CCAGAGCCGATTATCGATCGCAAACGCCAGCGGCC‑GATCTGGGCGCTGGATCTGATTCTGCTACCGCTGGTCGGGTTTGACGAA‑GTGGGCGGCCGCCTggg                                                                               >  1:226432/1‑149 (MQ=255)
                                                           aGCGTTTTGAGCAGGGTGAGAAGCTCAAGCCCAACCGCTTCCGTATT‑CCAGAGCCGATTATCGATCGCAAACGCCAGCGGCC‑GATCTGGGCGCTGGATCTGATTCTGCTACCGCTGGTCGGGTTTGACGAA‑GTGGGCGgccgc                                                                                    >  7:43364/1‑143 (MQ=255)
                                                           aGCGTTTTGAGCAGGGTGAGAAGCTCAAGCCCAACCGCTTCCGTATT‑CCAGAGCCGATTATCGATCGCAAACGCCAGCGGCC‑GATCTGGGCGCTGGATCTGATTCTGCTACCGCTGGTCGGGTTTGACGAA‑GTGGGCGgccgc                                                                                    <  8:43364/143‑1 (MQ=255)
                                                              gTTTTGAGCAGGGTGAGAAGCTCAAGCCCAACCGCTTCCGTATT‑CCAGAGCCGATTATCGATCGCAAACGCCAGCGGCC‑GATCTGGGCGCTGGATCTGATTCTGCTACCGCTGGTCGGGTTTGACGAA‑GTGGGCGGCCGCCTGGGTATg                                                                           <  5:20542/149‑1 (MQ=255)
                                                               ttttGAGCAGGGTGAGAAGCTCAAGCCCAACCGCTTCCGTATT‑CCAGAGCCGATTATCGATCGCAAACGCCAGCGGCC‑GATCTGGGCGCTGGATCTGATTCTGCTACCGCTGGTCGGGTTTGACGAA‑GTGGGCGGCCGCCTGGGTATgg                                                                          <  5:70602/149‑1 (MQ=255)
                                                                       aGGGTGAGAAGCTCAAGCCCAACCGCTTCCGTATT‑CCAGAGCCGATTATCGATCGCAAACGCCAGCGGCC‑GATCTGGGCGCTGGATCTGATTCTGCTACCGCTGGTCGGGTTTGACGAA‑GTGGGCGGCCGCCTGGGTATGGGCGGTGGc                                                                  <  2:226432/149‑1 (MQ=255)
                                                                             agaAGCTCAAGCCCAACCGCTTCCGTATT‑CCAGAGCCGATTATCGATCGCAAACGCCAGCGGCC‑GATCTGGGCGCTGGATCTGATTCTGCTACCGCTGGTCGGGTTTGACGAA‑GTGGGCGGCCGCCTGGGTATGGGCGGTGGCTTCTa                                                             <  1:144796/148‑1 (MQ=255)
                                                                             agaAGCTCAAGCCCAACCGCTTCCGTATT‑CCAGAGCCGATTATCGATCGCAAACGCCAGCGGCC‑GATCTGGGCGCTGGATCTGATTCTGCTACCGCTGGTCGGGTTTGACGAA‑GTGGGCGGCCGCCTGGGTATGGGCGGTGGCTTCTa                                                             >  2:144796/1‑148 (MQ=255)
                                                                             agaAGCTCAAGCCCAACCGCTTCCGTATT‑CCAGAGCCGATTATCGATCGCAAACGCCAGCGGCC‑GATCTGGGCGCTGGATCTGATTCTGCTACCGCTGGTCGGGGTTGACGAA‑GTGGGCGGCCGCCTGGGTATGGGCGGTGGCTTCTAt                                                            >  7:150575/1‑149 (MQ=255)
                                                                              gaAGCTCAAGCCCAACCGCTTCCGTATT‑CCAGAGCCGATTATCGATCGCAAACGCCAGCGGCC‑GATCTGGGCGCTGGATCTGATTCTGCTACCGCTGGTCGGGTTTGACGAA‑GTGGGCGGCCGCCTGGGTATGGGCGGTGGCTTCTAt                                                            >  7:267890/1‑148 (MQ=255)
                                                                                   tCAAGCCCAACCGCTTCCGTATT‑CCAGAGCCGATTATCGATCGCAAACGCCAGCGGCC‑GATCTGGGCGCTGGATCTGATTCTGCTACCGCTGGTCGGGTTTGACGAA‑GTGGGCGGCCGCCTGGGTATGGGCGGTGGCTTCTATGATCg                                                       >  6:322270/1‑148 (MQ=255)
                                                                                   tCAAGCCCAACCGCTTCCGTATT‑CCAGAGCCGATTATCGATCGCAAACGCCAGCGGCC‑GATCTGGGCGCTGGATCTGATTCTGCTACCGCTGGTCGGGTTTGACGAA‑GTGGGCGGCCGCCTGGGTATGGGCGGTGGCTTCTATGATCg                                                       >  3:319435/1‑148 (MQ=255)
                                                                                   tCAAGCCCAACCGCTTCCGTATT‑CCAGAGCCGATTATCGATCGCAAACGCCAGCGGCC‑GATCTGGGCGCTGGATCTGATTCTGCTACCGCTGGTCGGGTTTGACGAA‑GTGGGCGGCCGCCTGGGTATGGGCGGTGGCTTCTATGATCg                                                       >  5:6008/1‑148 (MQ=255)
                                                                                          cAACCGCTTCCGTATT‑CCAGAGCCGATTATCGTTCGCATACGCGAGCGGCC‑GATCTGGGCGCTGGATCTGATTCTGCTACCGCTGGTCGGGTTTGACGAA‑GTGGGCGGCCGCCTGGGTATGGGCGGTGGCtt                                                                <  4:115205/132‑1 (MQ=255)
                                                                                          cAACCGCTTCCGTATT‑CCAGAGCCGATTATCGATCGCAAACGCCAGCGGCC‑GATCTGGGCGCTGGATCTGATTCTGCTACCGCTGGTCGGGTTTGACGAA‑GTGGGCGGCCGCCTGGGTATGGGCGGTGGCtt                                                                >  3:115205/1‑132 (MQ=255)
                                                                                           aaCCGCTTCCGTATT‑CCAGAGCCGATTATCGATCGCAAACGCCAGCGGCC‑GATCTGGGCGCTGGATCTGATTCTGCTACCGCTGGTCGGGTTTGACGAATGTGGGCGGCCGCCTGGGTATGGGCGGTGGCTTCt                                                              <  1:47702/134‑1 (MQ=255)
                                                                                           aaCCGCTTCCGTATT‑CCAGAGCCGATTATCGATCGCAAACGCCAGCGGCC‑GATCTGGGCGCTGGATCTGATTCTGCTACCGCTGGTCGGGTTTGACGAA‑GTGGGCGGCCGCCTGGGTATGGGCGGTGGCTTCt                                                              >  2:47702/1‑133 (MQ=255)
                                                                                             ccGCTTCCGTATT‑CCAGAGCCGATTATCGATCGCAAACGTCAGCGGCC‑GATCTGGGCGCTGGATCTGATTCTGCTACCGCTGGTCGGGTTTGACGAA‑GTGGGCGGCCGCCTGGGTATgg                                                                          <  1:146967/119‑1 (MQ=255)
                                                                                             ccGCTTCCGTATT‑CCAGAGCCGATTATCGATCGCAAACGTCAGCGGCC‑GATCTGGGCGCTGGATCTGATTCTGCTACCGCTGGTCGGGTTTGACGAA‑GTGGGCGGCCGCCTGGGTATgg                                                                          >  2:146967/1‑119 (MQ=255)
                                                                                               gCTTCCGTATT‑CCAGAGCCGATTATCGATCGCAAACGCCAGCGGCC‑GATCTGGGCGCTGGATCTGATTCTGCTACCGCTGGTCGGGTTTGACGAA‑GTGGGCGGCCGCCTGGGTATGGGCGGTGGCTTCTATGATCGCAGCCTGGCCTAc                                          >  3:167450/1‑149 (MQ=255)
                                                                                                   ccGTATT‑CCAGAGCCGATTATCGATCGCAAACGCCAGCGGCC‑GATCTGGGCGCTGGATCTGATTCTGCTACCGCTGGTCGGGTTTGACGAA‑GTGGGCGGCCGCCTgg                                                                                >  7:297631/1‑107 (MQ=255)
                                                                                                   ccGTATT‑CCAGAGCCGATTATCGATCGCAAACGCCAGCGGCC‑GATCTGGGCGCTGGATCTGATTCTGCTACCGCTGGTCGGGTTTGACGAA‑GTGGGCGGCCGCCTgg                                                                                <  8:297631/107‑1 (MQ=255)
                                                                                                        tt‑CCAGAGCCGATTATCGATCGCAAACGCCAGCGGCC‑GATCTGGGCGCTGGATCTGATTCTGCTACCGCTGGTCGGGTTTGACGAA‑GTGGGCGGCCGCCTGGGTATGGGCGGTGGCTTCTATGATCGCAGCCTGGCCTACCAGGCGCGt                                 <  6:6008/149‑1 (MQ=255)
                                                                                                            cAGAGCCGATTATCGATCGCAAACGCCAGCGGCC‑GATCTGGGCGCTGGATCTGATTCTGCTACCGCTGGTCGGGTTTGACGAA‑GTGGGCGGCCGCCTGGGTATGGGCGGTGGCTTCTATGATCGCAGCCTGGCCTACCAGGCGCGTCgg                              >  3:127179/1‑149 (MQ=255)
                                                                                                                          gatcgCAAACGCCAGCGGCC‑GATCTGGGCGCTGGATCTGATTCTGCTACCGCTGGTCGGGTTTGACGAA‑GTGGGCGGCCGCCTGGGTATGGGCGGTGGCTTCTATGATCGCAGCCTGGCCTACCAGGCGCGTCGGCAAACCTGGaaaa                 >  5:27072/1‑148 (MQ=255)
                                                                                                                           atcgCAAACGCCAGCGGCC‑GATCTGGGCGCTGGATCTGATTCTGCTACCGCTGGTCGGGTTTGACGAA‑GTGGGCGGCCGCCTGGGTATGGGCGGTGGCTTCTATGATCGCAGCCTGGc                                              <  6:47868/118‑1 (MQ=255)
                                                                                                                           atcgCAAACGCCAGCGGCC‑GATCTGGGCGCTGGATCTGATTCTGCTACCGCTGGTCGGGTTTGACGAA‑GTGGGCGGCCGCCTGGGTATGGGCGGTGGCTTCTATGATCGCAGCCTGGc                                              >  5:47868/1‑118 (MQ=255)
                                                                                                                            tcgCAAACGCCAGCGGCC‑GATCTGGGCGCTGGATCTGATTCTGCTACCGCTGGTCGGGTTTGACGAA‑GTGGGCGGCCGCCTGGGTATGGGCGGTGGCTTCTATGATCGCAGCCTGGCCTACCAGGCGCGTCGGCAAACCt                       <  3:277286/140‑1 (MQ=255)
                                                                                                                            tcgCAAACGCCAGCGGCC‑GATCTGGGCGCTGGATCTGATTCTGCTACCGCTGGTCGGGTTTGACGAA‑GTGGGCGGCCGCCTGGGTATGGGCGGTGGCTTCTATGATCGCAGCCTGGCCTACCAGGCGCGTCGGCAAACCt                       >  4:277286/1‑140 (MQ=255)
                                                                                                                                aaaCGCCAGCGGCC‑GATCTGGGCGCTGGATCTGATTCTGCTACCGCTGGTCGGGTTTGACGAA‑GTGGGCGGCCGCCTGGGTATGGGCGGTGGCTTCTATGATCGCAGCCTGGCCTACCAGGCGCGTCGGCAAACCTGGAAAAAACCCtt          <  4:319435/149‑1 (MQ=255)
                                                                                                                                 aaCGCCAGCGGCC‑GATCTGGGCGCTGGATCTGATTCTGCTACCGCTGGTCGGGGTTGACGAA‑GTGGGCGGCCGCCTGGGTATGGGCGGTGGCTTCTATGATCGCAGCCTGGCCTACCAGGCGCGTCGGCAAACCTTGAAAAAACCCTtg         <  8:150575/149‑1 (MQ=255)
                                                                                                                                  aCGCCAGCGGCC‑GATCTGGGCGCTGGATCTGATTCTGCTACCGCTGGTCGGGTTTGACGAA‑GTGGGCGGCCGCCTGGGTATGGGCGGTGGCTTCTATGATCGCAGCCTGGCCTACCAGGCGCGTCGGCaaa                          <  3:145550/131‑1 (MQ=255)
                                                                                                                                  aCGCCAGCGGCC‑GATCTGGGCGCTGGATCTGATTCTGCTACCGCTGGTCGGGTTTGACGAA‑GTGGGCGGCCGCCTGGGTATGGGCGGTGGCTTCTATGATCGCAGCCTGGCCTACCAGGCGCGTCGGCaaa                          >  4:145550/1‑131 (MQ=255)
                                                                                                                                  aCGCCAGCGGCC‑GATCTGGGCGCTGGATCTGATTCTGCTACCGCTGGTCGGGTTTGACGAA‑GTGGGCGGCCGCCTGGGTATGGGCGGTGGCTTCTATGATCGCAGCCTGGCCTACCAGGCGCGTCGGCAAACCTGGAAAAAACCCTtgc        >  8:128612/1‑149 (MQ=255)
                                                                                                                                        gCGGCC‑GATCTGGGCGCTGGATCTGATTCTGCTACCGCTGGTCGGGTTTGACGAA‑GTGGGCGGCCGCCTGGGTATGGGCGGTGGCTTCTATGATCGCAGCCTGGCCTACCAGGCGCGTCGGCAAACCTGGAAAAAACCCTTGCTGCTgg  <  3:65803/149‑1 (MQ=255)
                                                                                                                                        gCGGCC‑GATCTGGGCGCTGGATCTGATTCTGCTACCGCTGGTCGGGTTTGACGAA‑GTGGGCGGCCGCCTGGGTATGGGCGGTGGCTTCTATGATCGCAGCCTGGCCTACCAGGCGCGTCGGCAAACCTGGAAAAAACCCTTGCTGCTgg  <  4:127179/149‑1 (MQ=255)
                                                                                                                                        gCGGCC‑GATCTGGGCGCTGGATCTGATTCTGCTACCGCTGGTCGGGTTTGACGAA‑GTGGGCGGCCGCCTGGGTATGGGCGGTGGCTTCTATGATCGCAGCCTGGCCTACCAGGCGCGTCGGCAAACCTGGAAAAAACCCTTGCTGCTgg  >  8:145250/1‑149 (MQ=255)
                                                                                                                                                aTCTGGGCGCTGGATCTGATTCTGCTACCGCTGGTCGGGTTTGACGAA‑GTGGGCGGCCGCCTGGGTATGGGCGGTGGCTTCTATGATCGCAGCCTGGCCTACCAGGCGCGTCGGCAAACCTGGAAAAAACCCTtgctg      >  4:344458/1‑138 (MQ=255)
                                                                                                                                                aTCTGGGCGCTGGATCTGATTCTGCTACCGCTGGTCGGGTTTGACGAA‑GTGGGCGGCCGCCTGGGTATGGGCGGTGGCTTCTATGATCGCAGCCTGGCCTACCAGGCGCGTCGGCAAACCTGGAAAAAACCCTtgctg      <  3:344458/138‑1 (MQ=255)
                                                                                                                                                      |                                                                                                                                        
CGGCAAGTGCATCTACCTGCCGGTGCTGCATGCCTGGCCGCGCACGCGCATGGTGTTCCAGCGTTTTGAGCAGGGTGAGAAGCTCAAGCCCAACCGCTTCCGTATT‑CCAGAGCCGATTATCGATCGCAAACGCCAGCGGCC‑GATCTGGGCGCTGGATCTGATTCTGCTACCGCTGGTCGGGTTTGACGAA‑GTGGGCGGCCGCCTGGGTATGGGCGGTGGCTTCTATGATCGCAGCCTGGCCTACCAGGCGCGTCGGCAAACCTGGAAAAAACCCTTGCTGCTGG  >  NC_002947/5935198‑5935481

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑