Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	5,938,098	T→C	9.1%	L220P (CTG→CCG)	rbbA →	ribosome‑associated ATPase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	5,938,098	0	T	C	9.1%	149.9 / 5.2	55	L220P (CTG→CCG)	rbbA	ribosome‑associated ATPase
Reads supporting (aligned to +/- strand):  ref base T (25/25);  new base C (3/2);  total (28/27)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.68e-01

CCACTGTCGCGGCGGCGCTTCTGGGAGCTGGTTGAACAGGTTCGCGCACAACGCCCGCAACTGACCCTGCT‑GGTGGCTACCGCCTACATGGAAGAAGCCGAACAGTTCGAGCA‑TTGCCTGATGCTCGATGGCGGCCGGCTGCTGGCCGCCGGCCCTAGCCACGAACTGGCTGCAGTCACCTCGAGCGGCAAACTGGATGATGCCTTTACCCATTACCAGGGCGCGGGCAAGGCGCAGCATCAACCACTGGCAATCCCGCCGCGCCAAGCCACGGACGG  >  NC_002947/5937956‑5938233                                                                                                                                                 |                                                                                                                                        ccACTGTCGCGGCGGCGCTTCTGGGAGCTGGTTGAACAGGTTCGCGCACAACGCCCGCAACTGACCCTGCT‑GGTGGCTACCGCCTACATGGAAGAAGCCGAACAGTTCGAGCA‑TTGCCTGATGCTCGATGGCGGCCGGCTGCTGgccgc                                                                                                                                   >  1:17408/1‑149 (MQ=255)   aCTGTCGCGGCGGCGCTTCTGGGAGCTGGTTGAACAGGTTCGCGCACAACGCCCGGAACTGACCCTGCT‑GGTGGCTACCGCCTACATGGAAGAAGCCGAACAGTTCGAGCA‑TTGCCTGATGCTCGATGGCGGCCGGCTGCTGGccgccg                                                                                                                                 <  3:74948/149‑1 (MQ=255)   aCTGTCGCGGCGGCGCTTCTGGGAGCTGGTTGAACAGGTTCGCGCACAACGCCCGCAACTGACCCTGCT‑GGTGGCTACCGCCTACATGGAAGAAGCCGAACAGTTCGAGCA‑TTGCCTGATGCTCGATGGCGGCCAGCAGCCGgccgcca                                                                                                                                 >  4:301453/1‑148 (MQ=255)     tGTCGCGGCGGCGCTTCTGGGAGCTGGTTGAACAGGTTCGCGCACAACGCCCGCAACTGACCCTGCT‑GGTGGCTACCGCCTACATGGAAGAAGCCGAACAGTTCGAGCA‑TTGCCTGATGCTCGATGGCGGCCGGCTGCTGGCCGCCGGc                                                                                                                               >  5:268161/1‑149 (MQ=255)       tCGCGGCGGCGCTTCTGGGAGCTGGTTGAACAGGTTCGCGCACAACGCCCGCAACTGACCCTGCT‑GGTGGCTACCGCCTACATGGACGAAGCCGAACAGTTCGAGCA‑TTGCCTGATGCTCGATGGCGGCCGGCTGCTGGCCGCCGGcc                                                                                                                              <  3:16254/148‑1 (MQ=255)       tCGCGGCGGCGCTTCTGGGAGCTGGTTGAACAGGTTCGCGCACAACGCCCGCAACTGACCCTGCT‑GGTGGCTACCGCCTACATGGAAGAAGCCGAACAGTTCGAGCA‑TTGCCTGATGCTCGATGGCGGCCAGCAGCCGgccgccatcga                                                                                                                             <  3:301453/149‑6 (MQ=255)         gcggcggcgCTTCTGGGAGCTGGTTGAACAGGTTCGCGCACAACGCCCGCAACTGACCCTGCT‑GGTGGCTACCGCCTACATGGAAGAAGCCGAACAGTTCGAGCA‑TTGCCTGATGCTCGATGGCGGCCGGCTGCTGGCCGCCGGCCCTa                                                                                                                           <  2:17408/149‑1 (MQ=255)                 gcTTCTGGGAGCTGGTTGAACAGGTTCGCGCACAACGCCCGCAACTGACCCTGCT‑GGTGGCTACCGCCTACATGGAAGAAGCCGAACAGTTCGAGCA‑TTGCCTGATGCTCGATGGCGGCCGGCTGCTGGCCGCCGGCCCTAGCCACGaa                                                                                                                   >  5:323224/1‑149 (MQ=255)                       gggAGCTGGTTGAACAGGTTCGCGCACAACGCCCGCAACTGACCCTGCT‑GGTGGCTACCGCCTACATGGAAGAAGCCGAACAGTTCGAGCA‑TTGCCTGATGCTCGATGGCGGCCGGCTGCTGGCCGCCGGCCCTAGCCACGAACTGGCt                                                                                                             >  7:207839/1‑149 (MQ=255)                        ggAGCTGGTTGAACAGGTTCGCGCACAACGCCCGCAACTGACCCTGCT‑GGTGGCTACCGCCTACATGGAAGAAGCCGAACAGTTCGAGCA‑TTGCCTGATGCTCGATGGCGGCCGGCTGCTGGCCGCCGGCCCTAGCCACGaa                                                                                                                   >  8:207963/1‑142 (MQ=255)                        ggAGCTGGTTGAACAGGTTCGCGCACAACGCCCGCAACTGACCCTGCT‑GGTGGCTACCGCCTACATGGAAGAAGCCGAACAGTTCGAGCA‑TTGCCTGATGCTCGATGGCGGCCGGCTGCTGGCCGCCGGCCCTAGCCACGaa                                                                                                                   <  7:207963/142‑1 (MQ=255)                        ggAGCTGGTTGAACAGGTTCGCGCACAACGCCCGCAACTGACCCTGCT‑GGTGGCTACCGCCTACATGGAAGAAGCCGAACAGTTCGAGCA‑TTGCCTGATGCTCGATGGCGGCCGGCTGCTGGCCGCCGGCCCTAGCCACGAACTGGCTg                                                                                                            <  4:343308/149‑1 (MQ=255)                              ggTTGAACAGGTTCGCGCACAACGCCCGCAACTGACCCTGCT‑GGTGGCTACCGCCTACATGGAAGAAGCCGAACAGTTCGAGCA‑TTGCCTGATGCTCGATGGCGGCCGGCTGCTGGCCGCCGGCCCTAGCCACGAACTGGCTGCAGTCa                                                                                                      >  5:317899/1‑149 (MQ=255)                               gTTGAACAGGTTCGCGCACAACGCCCGCAACTGACCCTGCT‑GGTGGCTACCGCCTACATGGAAGAAGCCGAACAGTTCGAGCA‑TTGCCTGATGCTCGATGGCGGCCGGCTGCTGGCCGCCGGCCCTAGCCACGAACTGGCTGCAGTCAc                                                                                                     >  7:311555/1‑149 (MQ=255)                                   aaCAGGTTCGCGCACAACGCCCGCAACTGACCCTGCT‑GGTGGCTACCGCCTACATGGAAGAAGCCGAACAGTTCGAGCA‑TTGCCTGATGCTCGATGGCGGCCGGCTGCTGGCCGCCGGCCCTAGCCACGAACTGGCTGCAGTCACGTCg                                                                                                 >  6:347584/1‑149 (MQ=255)                                   aaCAGGTTCGCGCACAACGCCCGCAACTGACCCTGCT‑GGTGGCTACCGCCTACATGGAAGAAGCCGAACAGTTCGAGCA‑TTGCCTGATGCTCGATGGCGGCCGGCTGCTGGCCGCCGGCCCTAGCCACGAACTGGCTGCAGTCACCTc                                                                                                  <  8:207839/148‑1 (MQ=255)                                   aaCAGGATCGCGCACAACGCCCGCAACTGACCCTGCT‑GGTGGCTACCGCCTACATGGAAGACGCCGAACAGTTCGAGCA‑TTGCCTGATGCTCGATGGCGGCCGGCTGCTGGCCGCCGGCCCTAGCCACGAACTgg                                                                                                               <  7:80369/135‑1 (MQ=255)                                        gTTCGCGCACAACGCCCGCAACTGACCCTGCT‑GGTGGCTACCGCCTACATGGAAGAAGCCGAACAGTTCGAGCA‑TTGCCTGATGCTCGATGGCGGCCGGCTGCTGGCCGCCGGCCCTAGCCACGAACTGGCTGCAGTCACCTCGAGCgg                                                                                            >  3:4111/1‑149 (MQ=255)                                            gcgcACAACGCCCGCAACTGACCCTGCTGGGTGGCTACCGCCTACATGGAAGAAGCCGAACAGTTCGAGCA‑TTGCCTGATGCTCGATGGCGGCCGGCTGCTGGCCGCCGGCCCTAGCCACGAACTGGCTGCAGTCACCTCGAGCGGCa                                                                                          >  1:198013/1‑148 (MQ=255)                                            gcgcACAACGCCCGCAACTGACCCTGCT‑GGTGGCTACCGCCTACATGGAAGAAGCCGAACAGTTCGAGCA‑TTGCCTGATGCTCGATGGCGGCCGGCTGCTGGCCGCCGGCCCTAGCCACGAACTGGCTGCAGTCACCTCGAGCGGCaaa                                                                                        >  3:117478/1‑149 (MQ=255)                                              gcACAACGCCCGCAACTGACCCTGCT‑GGTGGCTACCGCCTACATGGAAGAAGCCGAACAGTTCGAGCA‑TTGCCTGATGCTCGATGGCGGCCGGCTGCTGGCCGCCGGCCCTAGCCACGAACTGGCTGCAGTCACCTCGAGCGGCAAACt                                                                                      <  6:268161/149‑1 (MQ=255)                                              gcACAACGCCCGCAACTGACCCTGCT‑GGTGGCTACCGCCTACATGGAAGAAGCCGAACAGTTCGAGCA‑TTGCCTGATGCTCGATGGCGGCCGGCTGCTGGCCGCCGGCCCTAGCCACGAACTGGCTGCAGTCACCTCGAGCGGCAAACt                                                                                      >  8:298598/1‑149 (MQ=255)                                                     gCCCGCAACTGACCCTGCT‑GGTGGCTACCGCCTACATGGAAGAAGCCGAACAGTTCGAGCA‑TTGCCTGATGCTCGATGGCGGCCGGCTGCTGGCCGCCGGCCCTAGCCACGAACTGGCTGCAGTCACCTCGAGCGGCAAACTGgatgat                                                                               <  8:311555/149‑1 (MQ=255)                                                       ccGCAACTGACCCTGCT‑GGTGGCTACCGCCTACATGGAAGAAGCCGAACAGTTCGAGCA‑TTGCCTGATGCTCGATGGCGGCCGGCTGCTGGCCGCCGGCCCTAGCCACGAACTGGCTGCAGTCACCTCGAGCGGCAAACTGGATGATGc                                                                             <  2:198013/149‑1 (MQ=255)                                                       ccGCAACTGACCCTGCT‑GGTGGCTACCGCCTACATGGAAGAAGCCGAACAGTTCGAGCA‑TTGCCTGATGCTCGATGGCGGCCGGCTGCTGGCCGCCGGCCCTAGCCACGAACTGGCTGCAGTCACCTCGAGCGGCAAACTGGATGATGc                                                                             <  4:117478/149‑1 (MQ=255)                                                        cGCAACTGACCCTGCT‑GGTGGCTACCGCCTACATGGAAGAAGCCGAACAGTTCGAGCA‑TTGCCTGATGCTCGATGGCGGCCGGCTGCTGGCCGCCGGCCCTAGCCACGAACTGGCTGCAGTCAc                                                                                                     >  1:44795/1‑124 (MQ=255)                                                        cGCAACTGACCCTGCT‑GGTGGCTACCGCCTACATGGAAGAAGCCGAACAGTTCGAGCA‑TTGCCTGATGCTCGATGGCGGCCGGCTGCTGGCCGCCGGCCCTAGCCACGAACTGGCTGCAGTCAc                                                                                                     <  2:44795/124‑1 (MQ=255)                                                             cTGACCCTGCT‑GGTGGCTACCGCCTACATGGAAGAAGCCGAACAGTTAGAGCA‑TTGCCTGATGCTCGATGGCGGCCGGCTGCTGGCCGCCGGCCCTAGCCACGAACTGGCTGCAGTCACCTCGAGCGGCAAACTGGATGATGCCTTTAc                                                                       <  2:101336/149‑1 (MQ=255)                                                              tGACCCTGCT‑GGTGGCTACCGCCTACATGGAAGAAGCCGAACAGTTCGAGCA‑TTGCCTGATGCTCGATGGCGGCCGGCTGCTGGCCGCCGGCCCTAGCCACGAACTGGCTGCAGTCACCTCGAGCGGCAAACTGGATGATGCCTTTAcc                                                                      >  6:34593/1‑149 (MQ=255)                                                                  ccTGCT‑GGTGGCTACCGCCTACATGGAAGAAGCCGAACAGTTCGAGCAGTTGCCTGATGCTCGATGGCGGCCGGCTGCTGGCCGCCGGCCCTAGCCACGAACTGGCTGCAGTCACCTCGAGCGGCAAACTGGATGATGCCTTTACCCAt                                                                   >  1:257799/1‑149 (MQ=255)                                                                   ctgct‑gGTGGCTACCGCCTACATGGAAGAAGCCGAACAGTTCGAGCA‑TTGCCTGATGCTCGATGGCGGCCGGCTGCTGGCCGCCGGCCCTAGCCACGAACTGGCTGCAGTCACCTCGAGCGGCAAACTGGATGATGCCTTTACCCATTa                                                                 >  2:12427/1‑149 (MQ=255)                                                                    tgct‑gGTGGCTACCGCCTACATGGAAGAAGCCGAACAGTTCGAGCA‑TTGCCTGATGCTCGATGGCGGCCGGCTGCTGGCCGCCGGCCCTAGCCACGAACTGGCTGCAGTCACCTCGAGCGGCAAACTGGATGATGCCTTTACCCATTAc                                                                >  4:49195/1‑149 (MQ=255)                                                                               tACCGCCTACATGGAAGAAGCCGAACAGTTCGAGCA‑TTGCCTGATGCTCGATGGCGGCCGGCTGCTGGCCGCCGGCCCTAGCCACGAACTGGCTGCAGTCACCTCGAGCGGCAAACTGGATGATGCCTTTACCCAt                                                                   >  7:335200/1‑136 (MQ=255)                                                                               tACCGCCTACATGGAAGAAGCCGAACAGTTCGAGCA‑TTGCCTGATGCTCGATGGCGGCCGGCTGCTGGCCGCCGGCCCTAGCCACGAACTGGCTGCAGTCACCTCGAGCGGCAAACTGGATGATGCCTTTACCCAt                                                                   <  8:335200/136‑1 (MQ=255)                                                                               tACCGCCTACATGGAAGAAGCCGAACAGTTCGAGCA‑TTGCCTGATGCTCGATGGCGGCCGGCTGCTGGCCGCCGGCCCTAGCCACGAACTGGCTGCAGTCACCTCGAGCGGCAAACTGGATGATGCCTTTACCCATTACCAGGGCGCgg                                                      >  1:286966/1‑149 (MQ=255)                                                                                 ccGCCTACATGGAAGAAGCCGAACAGTTCGAGCA‑TTGCCTGATGCTCGATGGCGGCCGGCTGCTGGCCGCCGGCCCTAGCCACGAACTGGCTGCAGTCACCTCGAGCGGCAAACTGGATGATGCCTTTACCCATTACCAGGGCGCgg                                                      <  7:298598/147‑1 (MQ=255)                                                                                   gCCTACATGGAAGAAGCCGAACAGTTCGAGCA‑TTGCCTGATGCTCGATGGCGGCCGGCTGCTGGCCGCCGGCCCTAGCCACGAACTGGCTGCAGTCACCTCGAGCGGCAAACTGGATGATGCCTTTACCCATTACCAGGGCGCGGGc                                                    >  3:55727/1‑147 (MQ=255)                                                                                    ccTACATGGAAGAAGCCGAACAGTTCGAGCA‑TTGCCTGATGCTCGATGGCGGCCGGCTGCTGGCCGCCGGCCCTAGCCACGAACTGGCTGCAGTCACCTCGAGCGGCAAACTGGATGATGCCTTTACCCATTACCAGGGCGCGGGCAAg                                                 <  3:49195/149‑1 (MQ=255)                                                                                        cATGGAAGAAGCCGAACAGTTCGAGCA‑TTGCCTGATGCTCGATGGCGGCCGGCTGCTGGCCGCCGGCCCTAGCCACGAACTGGCTGCAGTCACCTCGAGCGGCAAACTGGATGATGCCTTTACCCATTACCAGGGCGCGGGCAAGgcg                                              <  4:4111/148‑1 (MQ=255)                                                                                         aTGGAAGAAGCCGAACAGTTCGAGCA‑TTGCCTGATGCTCGATGGCGGCCGGCTGCTGGCCGCCGGCCCTAGCCACGAACTGGCTGCAGTCACCTCGAGCGGCAAACTGGATGATGCCTTTACCCATTACCAGGGCGCGGGCAAGgcg                                              >  5:316286/1‑147 (MQ=255)                                                                                         aTGGAAGAAGCCGAACAGTTCGAGCA‑TTGCCTGATGCTCGATGGCGGCCGGCTGCTGGCCGCCGGCCCTAGCCACGAACTGGCTGCAGTCACCTCGAGCGGCAAACTGGATGATGCCTTTACCCATTACCAGGGCGCGGGCAAGGCgca                                            <  6:323224/149‑1 (MQ=255)                                                                                          tGGAAGAAGCCGAACAGTTCGAGCA‑TTGCCTGATGCTCGATGGCGGCCGGCTGCTGGCCGCCGGCCCTAGCCACGAACTGGCTGCAGTCACCTCGAGCGGCAAACTGGATGATGCCTTTACCCATTACCAGGGCGCGGGCAAGGCgcag                                           <  4:55727/149‑1 (MQ=255)                                                                                                 agCCGAACAGTTCGAGCA‑TTGCCTGATGCTCGATGGCGGCCGGCTGCTGGCCGCCGGCCCTAGCCACGAACTGGCTGCAGTCACCTCGAGCGGCAAACTGGATGATGCCTTTACCCATTa                                                                 <  5:224243/120‑1 (MQ=255)                                                                                                 agCCGAACAGTTCGAGCA‑TTGCCTGATGCTCGATGGCGGCCGGCTGCTGGCCGCCGGCCCTAGCCACGAACTGGCTGCAGTCACCTCGAGCGGCAAACTGGATGATGCCTTTACCCATTa                                                                 >  6:224243/1‑120 (MQ=255)                                                                                                 agCCGAACAGTTCGAGCA‑TTGCCTGATGCTCGATGGCGGCCGGCTGCTGGCCGCCGGCCCTAGCCACGAACTGGCTGCAGTCACCTCGAGCGGCAAACTGGATGATGCCTTTACCCATTACCAGGGCGCGGGCAAGGCGCAGCATCAAc                                    <  5:34593/149‑1 (MQ=255)                                                                                                 agCCGAACAGTTCGAGCA‑TTGCCTGATGCTCGATGGCGGCCGGCTGCTGGCCGCCGGCCCTAGCCACGAACTGGCTGCAGTCACCTCGAGCGGCAAACTGGATGATGCCTTTACCCATTACCAGGGCGCGGGCAAGGCGCAGCATCAAc                                    <  6:316286/149‑1 (MQ=255)                                                                                                           ttCGAGCA‑TTGCCTGATGCTCGATGGCGGCCGGCTGCTGGCCGCCGGCCCTAGCCACGAACTGGCTGCAGTCACCTCGAGCGGCAAACTGGATGATGCCTTTACCCATTACCAGGGCGCGGGCAAGGCGCAGCATCAACCACTGGCAAt                          >  3:305682/1‑149 (MQ=255)                                                                                                              gAGCA‑TTGCCTGATGCTCGATGGCGGCCGGCTGCTGGCCGCCGGCCCTAGCCACGAACTGGCTGCAGTCACCTCGAGCGGCAAACTg                                                                                     >  7:228765/1‑87 (MQ=255)                                                                                                              gAGCA‑TTGCCTGATGCTCGATGGCGGCCGGCTGCTGGCCGCCGGCCCTAGCCACGAACTGGCTGCAGTCACCTCGAGCGGCAAACTg                                                                                     <  8:228765/87‑1 (MQ=255)                                                                                                                      gCCTGATGCTCGATGGCGGCCGGCTGCTGGCCGCCGGCCCTAGCCACGAACTGGCTGCAGTCACCTCGAGCGGCAAACTGGATGATGCCTTTACCCATTACCAGGGCGCGGGCAAGGCGCAGCATCAACCACTGGCAATCCCGCCgcgc                >  1:330394/1‑149 (MQ=255)                                                                                                                           aTGCTCGATGGCGGCCGGCTGCTGGCCGCCGGCCCTAGCCACGAACTGGCTGCAGTCACCTCGAGCGGCAAACTGGATGATGCCTTTACCCATTACCAGGGCGCGGGCAAGGCGCAGCATCAACCACTGGCAATCCCGCCGCGCCaa             <  2:257799/147‑1 (MQ=255)                                                                                                                                 gATGGCGGCCGGCTGCTGGCCGCCGGCCCTAGCCACGAACTGGCTGCAGTCACCTCGAGCGGCAAACTGGATGATGCCTTTACCCATTACCAGGGCGCGGGCAAGGCGCAGCATCAACCACTGGCAATCCCGCCGCGCCAAGCCacgga     <  4:305682/149‑1 (MQ=255)                                                                                                                                    tagggccggcGGCCAGCAGCCGGCCCTAGCCACGAACTGGCTGCAGTCACCTCGAGCGGCAAACTGgatgat                                                                               <  2:207996/69‑1 (MQ=37)                                                                                                                                    tagggccggcGGCCAGCAGCCGGCCCTAGCCACGAACTGGCTGCAGTCACCTCGAGCGGCAAACTGgatgat                                                                               >  1:207996/4‑72 (MQ=37)                                                                                                                                    tagggccggcGGCCAGCAGCCGGCCCTAGCCACGAACTGGCTGCAGTCACCTCGAGCGGCAAACTGGATGATGCCTTTACCCATTACCAGGGCGCGGGCAAGGCGCAGCATCAACCACTGGCAATCCCGCCGCGCCAAGCCacggacgg  >  1:162029/4‑149 (MQ=255)                                                                                                                                                 tgGCCGCCGGCCCTAGCCACGAACTGGCTGCAGTCACCTCGAGCGGCAAACTGGATGATGCCTTTACCCATTACCAGGGCGCGGGCAAGGCGCAGCATCAAc                                    >  4:100388/1‑102 (MQ=255)                                                                                                                                                 tgGCCGCCGGCCCTAGCCACGAACTGGCTGCAGTCACCTCGAGCGGCAAACTGGATGATGCCTTTACCCATTACCAGGGCGCGGGCAAGGCGCAGCATCAAc                                    <  3:100388/102‑1 (MQ=255)                                                                                                                                                 |                                                                                                                                        CCACTGTCGCGGCGGCGCTTCTGGGAGCTGGTTGAACAGGTTCGCGCACAACGCCCGCAACTGACCCTGCT‑GGTGGCTACCGCCTACATGGAAGAAGCCGAACAGTTCGAGCA‑TTGCCTGATGCTCGATGGCGGCCGGCTGCTGGCCGCCGGCCCTAGCCACGAACTGGCTGCAGTCACCTCGAGCGGCAAACTGGATGATGCCTTTACCCATTACCAGGGCGCGGGCAAGGCGCAGCATCAACCACTGGCAATCCCGCCGCGCCAAGCCACGGACGG  >  NC_002947/5937956‑5938233

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑