Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 5,949,920 G→C 8.2% A213P (GCT→CCT) ‡ ppx → exopolyphosphatase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029475,949,9200GC 8.2% 143.1 / 4.3 49A213P (GCT→CCT) ‡ppxexopolyphosphatase
Reads supporting (aligned to +/- strand):  ref base G (24/20);  new base C (2/2);  total (26/23)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.10e-01

AGATGGGTTGCGTGAGCTTTACCCAGCGCTATTTCCGCGACGGCAAGATCACCCCGGCCCGCTATGCCCAGGCCTACACTGCCGCACGCCTGGAACTGATGAGCATCGAAAATGCCCTGCACCGCCTGACCTGGGATGAGGCTATCGGCTCATCCGGCACCATTCGCGCCATCGGCGCGGCCATCAAGGCCGGCGGCCTGGGCAATGGCGAGGTCAACGCCGAAGGCCTGGCCTGGGTCAAACGCAAGCTGTTCAAGCTGGGCGAAGTCGACAAGATCGACTTCGACGG  >  NC_002947/5949780‑5950068
                                                                                                                                            |                                                                                                                                                    
aGATGGGTTGCGTGAGCTTTACCCAGCGCTATTTCCGCGACGGCAAGATCACCCCGGCCCGCTATGCCCAGGCCTACACTGCCGCACGCCTGGAACTGATGAGCATCGAAAATGCCCTGCACCGCCTGACCTGGGATGAGGCTATCGGc                                                                                                                                              <  6:357345/149‑1 (MQ=255)
     ggTTGCGTGAGCTTTACCCAGCGCTATTTCCGCGACGGCAAGATCACCCCGGCCCGCTATGCCCAGGCCTACACTGCCGCACGCCTGGAACTGATGAGCATCGATAATGCCCTGCACCGCCTGACCTGGGATGAGGCTATCGGCTCATc                                                                                                                                         <  7:267559/149‑1 (MQ=255)
      gTTGCGTGAGCTTTACCCAGCGCTATTTCCGCGACGGCAAGATCACCCCGGCCCGCTATGCCCAGGCCTACACTGCCGCACGCCTGGAACTGATGAGCATCGAAAATGCCCTGCACCGCCTGACCTGGGATGAGGCTATCGGCTCATcc                                                                                                                                        >  4:261496/1‑149 (MQ=255)
                cTTTACCCAGCGCTATTTCCGCGACGGCAAGATCACCCCGGCCCGCTATGCCCAGGCCTACACTGCCGCACGCCTGGAACTGATGAGCATCGAAAATGCCCTGCACCGCCTGACCTGGGATGAGGCTATCGGCTCATCCGGCACCATTc                                                                                                                              >  3:78941/1‑149 (MQ=255)
                   tACCCAGCGCTATTTCCGCGACGGCAAGATCACCCCGGCCCGCTATGCCCAGGCCTACACTGCCGCACGCCTGGAACTGATGAGCATCGAAAATGCCCTGCACCGCCTGACCTGGGATGAGGCTATCGGCTCATCCGGCACCATTcgc                                                                                                                            <  1:79709/148‑1 (MQ=255)
                        aGCGCTATTTCCGCGACGGCAAGATCACCCCGGCCCGCTATGCCCAGGCCTACACTGCCGCACGCCTGGAACTGATGAGCATCGAAAATGCCCTGCACCGCCTGACCTGGGATGAGGCTATCGGCTCATCCGGCACCATTCGCGCCATc                                                                                                                      >  5:76008/1‑149 (MQ=255)
                        aGCGCTATTTCCGCGACGGCAAGATCACCCCGGCCCGCTATGCCCAGGCCTACACTGCCGCACGCCTGGAACTGATGAGCATCGAAAATGCCCTGCACCGCCTGACCTGGGATGAGGCTATCGGCTCATCCGGCACCATTCGCGCCATc                                                                                                                      >  7:249157/1‑149 (MQ=255)
                              aTTTCCGCGACGGCAAGATCACCCCGGCCCGCTATGCCCAGGCCTACACTGCCGCACGCCTGGAACTGATGAGCATCGAAAATGCCCTGCACCGCCTGACCTGGGATGAGGCTATCGGCTCATCCGGCACCATTCGCGCCATCGGcgcg                                                                                                                >  5:76420/1‑149 (MQ=255)
                               tttCCGCGACGGCAAGATCACCCCGGCCCGCTATGCCCAGGCCTACACTGCCGCACGCCTGGAACTGATGAGCATCGAAAATGCCCTGCACCGCCTGACCTGGGATGAGGCTATCGGCTCATCCGGCACCATTCGCGCCATCGGCGCgg                                                                                                               >  3:300273/1‑149 (MQ=255)
                                            aaGATCACCCCGGCCCGCTATGCCCAGGCCTACACTGCCGCACGCCTGGAACTGATGAGCATCGAAAATGCCCTGCACCGCCTGACCTGGGATGAGGCTATCGGCTCATc                                                                                                                                         <  5:68456/110‑1 (MQ=255)
                                            aaGATCACCCCGGCCCGCTATGCCCAGGCCTACACTGCCGCACGCCTGGAACTGATGAGCATCGAAAATGCCCTGCACCGCCTGACCTGGGATGAGGCTATCGGCTCATc                                                                                                                                         >  6:68456/1‑110 (MQ=255)
                                            aaGATCACCCCGGCCCGCTATGCCCAGGCCTACACTGCCGCACGCCTGGAACTGATGAGCATCGAAAATGCCCTGCACCGCCTGACCTGGGATGAGGCTATCGGCTCATCCGGCACCATTCGCGCCATCGGCGCGGCCATCAAGGCcgg                                                                                                  >  7:170520/1‑149 (MQ=255)
                                            aaGATCACCCCGGCCCGCTATGCCCAGGCCTACACTGCCGCACGCCTGGAACTGATGAGCATCGAAAATGCCCTGCACCGCCTGACCTGGGATGAGCCGATAGCCTCATccc                                                                                                                                       >  7:178702/1‑111 (MQ=255)
                                            aaGATCACCCCGGCCCGCTATGCCCAGGCCTACACTGCCGCACGCCTGGAACTGATGAGCATCGAAAATGCCCTGCACCGCCTGACCTGGGATGAGCCGATAGCCTCATccc                                                                                                                                       <  8:178702/112‑2 (MQ=255)
                                                   ccccGGCCCGCTATGCCCAGGCCTACACTGCCGCACGCCTGGAACTGATGAGCATCGAAAATGCCCTGCACCGCCTGACCTGGGATGAGGCTATCGGCTCATCCGGCACCATTCGCGCCATCGGCGCGGCCATCAAg                                                                                                       >  3:13737/1‑137 (MQ=255)
                                                   ccccGGCCCGCTATGCCCAGGCCTACACTGCCGCACGCCTGGAACTGATGAGCATCGAAAATGCCCTGCACCGCCTGACCTGGGATGAGGCTATCGGCTCATCCGGCACCATTCGCGCCATCGGCGCGGCCATCAAg                                                                                                       <  4:13737/137‑1 (MQ=255)
                                                   ccccGGCCCGCTATGCCCAGGCCTACACTGCCGCACGCCTGGAACTGATGAGCATCGAAAATGCCCTGCACCGCCTGACCTGGGATGAGGCTATCGGCTCATCCGGCACCATTCGCGCCATCGGCGCGGCCATCAAGGCCGGCGGCCTg                                                                                           <  6:76420/149‑1 (MQ=255)
                                                    cccGGCCCGCTATGCCCAGGCCTACACTGCCGCACGCCTGGAACTGATGAGCATCGAAAATGCCCTGCACCGCCTGACCTGGGATGAGGCTATCGGCTCATCCGGCACCATTCGCGCCATCGGCGCGGCCATCAAgg                                                                                                      >  1:60694/1‑137 (MQ=255)
                                                    cccGGCCCGCTATGCCCAGGCCTACACTGCCGCACGCCTGGAACTGATGAGCATCGAAAATGCCCTGCACCGCCTGACCTGGGATGAGGCTATCGGCTCATCCGGCACCATTCGCGCCATCGGCGCGGCCATCAAgg                                                                                                      <  2:60694/137‑1 (MQ=255)
                                                    cccGGCCCGCTATGCCCAGGCCTACACTGCCGCACGCCTGGAACTGATGAGCATCGAAAATGCCCTGCACCGCCTGACCTGGGATGAGGCTATCGGCTCATCCGGCACCATTCGCGCCATCGGCGCGGCCATCAAGGCCGGCGGCCTgg                                                                                          >  3:317217/1‑149 (MQ=255)
                                                                tGCCCAGGCCTACACTGCCGCACGCCTGGAACTGATGAGCATCGAAAATGCCCTGCACCGCCTGACCTGGGATGAGGCTATCGGCTCATCCGGCACCATTCGCGCCATCGGCGCGGCCATCAAGGCCGGCGGCCTGGGCAATGGCGAgg                                                                              <  4:78941/149‑1 (MQ=255)
                                                                   ccAGGCCTACACTGCCGCACGCCTGGAACTGATGAGCATCGAAAATGCCCTGCACCGCCTGACCTGGGATGAGGCTATCGGCTCATCCGGCACCATTCGCGCCATCGGCGCGGCCATCaa                                                                                                        >  3:219439/1‑120 (MQ=255)
                                                                   ccAGGCCTACACTGCCGCACGCCTGGAACTGATGAGCATCGAAAATGCCCTGCACCGCCTGACCTGGGATGAGGCTATCGGCTCATCCGGCACCATTCGCGCCATCGGCGCGGCCATCaa                                                                                                        <  4:219439/120‑1 (MQ=255)
                                                                                 ccGCACGCCTGGAACTGATGAGCATCGAAAATGCCCTGCACCGCCTGACCTGGGATGAGGCTATCGGCTCATCCGGCACCATTCGCGCCATCGGCGCGGCCATCAAGGCCGGCGGCCTGGGCAATGGCGAGGTCAACGCCGAAggcctg                                                             >  4:180378/1‑149 (MQ=255)
                                                                                   gCACGCCTGGAACTGATGAGCATCGAAAATGCCCTGCACCGCCTGACCTGGGATGAGGCTATCGGCTCATCCGGCACCATTCGCGCCATCGGCGCGGCCATCAAGGCCGGCGGCCTGGGCAATGGCGAg                                                                               <  3:11676/129‑1 (MQ=255)
                                                                                   gCACGCCTGGAACTGATGAGCATCGAAAATGCCCTGCACCGCCTGACCTGGGATGAGGCTATCGGCTCATCCGGCACCATTCGCGCCATCGGCGCGGCCATCAAGGCCGGCGGCCTGGGCAATGGCGAg                                                                               >  4:11676/1‑129 (MQ=255)
                                                                                       gCCTGGAACTGATGAGCATCGAAAATGCCCTGCACCGCCTGACCTGGGATGAGGCTATCGGCTCATCCGGCACCATTCGCGCCATCGGCGCGGCCATCAAGGCCGGCGGCCTGGGCAATGGCGAGGTCAACGCCGAAGGcctggcctgg                                                       <  8:249157/149‑1 (MQ=255)
                                                                                          tGGAACTGATGAGCATCGAAAATGCCCTGCACCGCCTGACCTGGGATGAGGCTATCGGCTCATCCGGCACCATTCGCGCCATCGGCGCGGCCATCAAGGCCGGCGGCCTGGGCAATGGCGAGGTCAACGCCGAAGGCCTGGCCTGGGt                                                     >  7:314434/1‑148 (MQ=255)
                                                                                            gAACTGATGAGCATCGAAAATGCCCTGCACCTCCTGACCTGGGATGAGGCTATCGGCTCATCCGGCACCATTCGCGCCATCGGCGCGGCCATCAAGGCCGGCGGCCTGGGCAATGGCGAGGTCAACGCCGAAGGCCTGGCCTGGGTc                                                    <  6:76008/147‑1 (MQ=255)
                                                                                              aCTGATGAGCATCGAAAATGCCCTGCACCGCCTGACCTGGGATGAGGCTATCGGCTCATCCGGCACCATTCGCGCCATCGGCGCGGCCATCAAGGCCGGCGGCCTGGGCAATGGCGAGGTCAACGCCGAAGGCCTGGCCTGGGTCAAAc                                                <  8:170520/149‑1 (MQ=255)
                                                                                                   tgaGCATCGAAAATGCCCTGCACCGCCTGACCTGGGATGAGGCTATCGGCTCATCCGGCACCATTCGCGCCATCGGCGCGGCCATCAAGGCCGGCGGCCTGGGCAATGGCGAGGTCAACGCCGAAGGCCTGGCCTGGGTCAAACGCaa                                            <  3:180378/148‑1 (MQ=255)
                                                                                                      gCATCGAAAATGCCCTGCACCGCCTGACCTGGGATGAGGCTATCGGCTCATCCGGCACCATTCGCGCCATCGGCGCGGCCATCAAGGCCGGCGGCCTGGGCAATGGCGAGGTCAACGCCGAAGGCCTGGCCTGGGTCAAACGCAAGCTg                                        >  4:299318/1‑149 (MQ=255)
                                                                                                        aTCGAAAATGCCCTGCACCGCCTGACCTGGGATGAGGCTATCGGCTCATCCGGCACCATTCGCGCCATCGGCGCGGCCATCAAGGCCGGCGGCCTGGGCAATGGCGAGGTCAACGCCGAAGGCCTGGCCTGGGTCAAACGCaa                                            <  7:86124/143‑1 (MQ=255)
                                                                                                        aTCGAAAATGCCCTGCACCGCCTGACCTGGGATGAGGCTATCGGCTCATCCGGCACCATTCGCGCCATCGGCGCGGCCATCAAGGCCGGCGGCCTGGGCAATGGCGAGGTCAACGCCGAAGGCCTGGCCTGGGTCAAACGCaa                                            >  8:86124/1‑143 (MQ=255)
                                                                                                        aTCGAAAATGCCCTGCACCGCCTGACCTGGGATGAGGCTATCGGCTCATCCGGCACCATTCGCGCCATCGGCGCGGCCATCAAGGCCGGCGGCCTGGGCAATGGCGAGGTCAACGCCGAAGGCCTGGCCTGGGTCAAACGCAAGCTGtt                                      <  3:261496/149‑1 (MQ=255)
                                                                                                         tCGAAAATGCCCTGCACCGCCTGACCTGGGATGAGGCTATCGGCTCATCCGGCACCATTCGCGCCATCGGCGCGGCCATCAAGGCCGGCGGCCTGGGCAATGGCGAGGTCAACGCCGAAGGCCTGGCCTGGGTCAAACGCAAGCTGtt                                      >  3:276312/1‑148 (MQ=255)
                                                                                                         tCGAAAATGCCCTGCACCGCCCGACCTGGGATGAGGCTATCGGCTCATCCGGCACCATTCGCGCCATCGGCGCGGCCATCAAGGCCGGCGGCCTGGGCAATGGCGAGGTTAACGCCGAAGGCCTGGCCTGGGTCAAACGCAAGCTGtt                                      >  7:258489/1‑148 (MQ=255)
                                                                                                            aaaaTGCCCTGCACCGCCTGACCTGGGATGAGGCTATCGGCTCATCCGGCACCATTCGCGCCATCGGCGCGGCCATCAAGGCCGGCGGCCTgg                                                                                          <  5:153790/93‑1 (MQ=255)
                                                                                                            aaaaTGCCCTGCACCGCCTGACCTGGGATGAGGCTATCGGCTCATCCGGCACCATTCGCGCCATCGGCGCGGCCATCAAGGCCGGCGGCCTgg                                                                                          >  6:153790/1‑93 (MQ=255)
                                                                                                                tGCCCTGCACCGCCTGACCTGGGATGAGGCTATCGGCTCATCCGGCACCATTCGCGCCATCGGCGCGGCCATCAAGGCCGGCGGCCTGGGCAATGGCGAGGTCAACGCCGAAGGCCTGGCCTGGGTCAAACGCAAGCTGTTCAAGCTgg                              <  5:203919/149‑1 (MQ=255)
                                                                                                                       cACCGCCTGTCCTGGGATGAGTGTATCGGCTCATCGGGCTCTATTCGAGGCATTGGCGCGGCGATCAAGGTCGGCGGGCTGGGCAATGGCGAGGTCAACGCCGAAGGCCTGGCCTTGGTCAAAGGCAAGCTGTTCTAGCTGGGc                            <  2:254763/144‑1 (MQ=255)
                                                                                                                       cACCGCCTGACCTGGGATGAGGCTATCGGCTCATACGGCACCATTCGCGCCATCGGCGCGGCCATCAAGGCCGGCGGCCTGGGCAATGGCGAGGTCAACGCCGAAGGCCTGGCCTGGGTCAAACGCAAGCTGTTAAAGCTGGGc                            >  1:254763/1‑144 (MQ=255)
                                                                                                                          cGCCTGACCTGGGATGAGGCTATCGGCTCATCCGGCACCATTCGCGCCATCGGCGCGGCCATCAAGGCCGGCGGCCTGGGCAATGGCGAGGTCAACGCCGAAGGCCTGGCCTGGGTCAAACGCAAGCTGTTCAAGCTGGGCGAAGTCGa                    >  5:119919/1‑149 (MQ=255)
                                                                                                                           gCCTGACCTGGGATGAGGCTATCGGCTCATCCGGCACCATTCGCGCCATCGGCGCGGCCATCAAGGCCGGCGGCCTGGGCAATGGCGAGGTCAACGCCGAAGGCCTGGCCTGGGTCAAACGCAAGCTGTTCAAGCTGGGCGAATTCGAc                   <  3:299318/149‑1 (MQ=255)
                                                                                                                                  cTGGGATGAGGCTATCGGCTCATCCGGCACCATTCGCGCCATCGGCGCGGCCATCAAGGCCGGCGGCCTGGGCAATGGCGAGGTCAACGCCGAAGGCCTGGCCTGGGTCAAACGCAAGCTGTTCAAGCTGGGCGAAGTCGACAAGATc             >  3:227325/1‑148 (MQ=255)
                                                                                                                                     ggATGAGCCGATAGCCTCATCCGGCACCATTCGCGCCATCGGCGCGGCCATCAAGGCCGGCGGCCTGGGCAATGGCGAGGTCAACGCCGAAGGCCTGGCCTGGGTCAAACGCAAGCTGTTCAAGCTGGGCGAAGTCGACAAGATc             <  5:124485/145‑1 (MQ=255)
                                                                                                                                     ggATGAGCCGATAGCCTCATCCGGCACCATTCGCGCCATCGGCGCGGCCATCAAGGCCGGCGGCCTGGGCAATGGCGAGGTCAACGCCGAAGGCCTGGCCTGGGTCAAACGCAAGCTGTTCAAGCTGGGCGAAGTCGACAAGATc             >  6:124485/1‑145 (MQ=255)
                                                                                                                                      gATGAGGCTATCGGCTCATCCGGCACCATTCGCGCCATCGGCGCGGCCATCAAGGCCGGCGGCCTGGGCAATGGCGAGGTCAACGCCGAAGGCCTGGCCTGGGTCAAACGCAAGCTGTTCAAGCTGGGCGAAGTCGAc                   <  4:257279/138‑1 (MQ=255)
                                                                                                                                      gATGAGGCTATCGGCTCATCCGGCACCATTCGCGCCATCGGCGCGGCCATCAAGGCCGGCGGCCTGGGCAATGGCGAGGTCAACGCCGAAGGCCTGGCCTGGGTCAAACGCAAGCTGTTCAAGCTGGGCGAAGTCGAc                   >  3:257279/1‑138 (MQ=255)
                                                                                                                                           ggCTATCGGCTCATCCGGCACCATTCGCGCCATCGGCGCGGCCATCAAGGCCGGCGGCCTGGGCAATGGCGAGGTCAACGCCGAAGGCCTGGCCTGGGTCAAACGCAAGCTGTTCAAGCTgg                              >  2:12984/1‑122 (MQ=255)
                                                                                                                                           ggCTATCGGCTCATCCGGCACCATTCGCGCCATCGGCGCGGCCATCAAGGCCGGCGGCCTGGGCAATGGCGAGGTCAACGCCGAAGGCCTGGCCTGGGTCAAACGCAAGCTGTTCAAGCTgg                              <  1:12984/122‑1 (MQ=255)
                                                                                                                                           ggCTATCGGCTCATCCGGCACCATTCGCGCCATCGGCGCGGCCATCAAGGCCGGCGGCCTGGGCAATGGCGAGGTCAACGCCGAAGGCCTGGCCTGGGTCAAACGCAAGCTGTTCAAGCTGGGCGAAGTCGACAAGATCGACTTCGa     >  3:224005/1‑147 (MQ=255)
                                                                                                                                           ggCTATCGGCTCATCCGGCACCATTCGCGCCATCGGCGCGGCCATCAAGGCCGGCGGCCTGGGCAATGGCGAGGTCAACGCCGAAGGCCTGGCCTGGGTCAAACGCAAGCTGTTCAAGCTGGGCGAAGTCGACAAGATCGACTTCGa     <  4:224005/147‑1 (MQ=255)
                                                                                                                                            gCTATCGGCTCATCCGGCACCATTCGCGCCATCGGCGCGGCCATCAAGGCCGGCGGCCTGGGCAATGGCGAGGTCAACGCCGAAGGCCTGGCCTGGGTCAAACGCAAGCTGTTCAAGCTGGGCGAAGTCGACAAGATCGACTTCGACgg  >  7:186498/1‑149 (MQ=255)
                                                                                                                                            |                                                                                                                                                    
AGATGGGTTGCGTGAGCTTTACCCAGCGCTATTTCCGCGACGGCAAGATCACCCCGGCCCGCTATGCCCAGGCCTACACTGCCGCACGCCTGGAACTGATGAGCATCGAAAATGCCCTGCACCGCCTGACCTGGGATGAGGCTATCGGCTCATCCGGCACCATTCGCGCCATCGGCGCGGCCATCAAGGCCGGCGGCCTGGGCAATGGCGAGGTCAACGCCGAAGGCCTGGCCTGGGTCAAACGCAAGCTGTTCAAGCTGGGCGAAGTCGACAAGATCGACTTCGACGG  >  NC_002947/5949780‑5950068

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑