Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 5,953,291 A→G 5.5% E21G (GAA→GGA)  PP_5218 → DedA family protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029475,953,2910AG 5.5% 215.5 / 2.3 73E21G (GAA→GGA) PP_5218DedA family protein
Reads supporting (aligned to +/- strand):  ref base A (34/35);  new base G (2/2);  total (36/37)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.61e-01

ACGTCGAGACACATTTGGACACTTTCACGAACGCGCGCGAAGGGGTAGGCTGCGCGTTCATTTCGCCAGCACCTCAGAAAATGCTTCAACAATTCCTGCAGGATTTCGGCTACTTCGCCCTTTTTCTAGGCACCTTCTTTGAAGGCGAGACCATTCTGGTGCTTGCGGGATTCCTTGCGTTCCGCGGTTACATGGATATCAATCTGGTATGCCTGGTGGCCTTCTTCGGCAGCTACGCGGGCGACCAGTTGTGGTACTTCATGGGCCGACGCCACGGGCGCAGGA  >  NC_002947/5953150‑5953434
                                                                                                                                             |                                                                                                                                               
aCGTCGAGACACATTTGGACACTTTCACGAACGCGCGCGAAGGGGTAGGCTGCGCGTTCATTTCGCCAGCACCTCAGAAAATGCTTCAACAATTCCTGCAGGATTTCGGCTACTTCGCCCTTTTTCTAGGCACCTTCTTTGAAGGc                                                                                                                                             >  6:67637/1‑146 (MQ=255)
 cGTCGAGACACATTTGGACACTTTCACGAACGCGCGCGAAGGGGTAGGCTGCGCGTTCATTTCGCCAGCACCTCAGAAAATGCTTCAACAATTCCTGCAGGATTTCGGCTACTTCGCCCTTTTTCTAGGCACCTTCTTTGAAGGCgaga                                                                                                                                         <  2:176833/149‑1 (MQ=255)
  gTCGAGACACATTTGGACACTTTCACGAACGCGCGCGAAGGGGTAGGCTGCGCGTTCATTTCGCCAGCACCTCAGAAAATGCTTCAACAATTCCTGCAGGATTTCGGCTACTTCGCCCTTTTTCTAGGCACCTTCTTTGAAGGCGAGAc                                                                                                                                        >  2:64701/1‑149 (MQ=255)
      agaCACATTTGGACACTATCACGAACGCGCGCGAAGGGGTAGGCAGCGCGATCATTTCGCCAGCACCTCAGAAAAAGCTTCAACAATTCCTGCAGGATTTCGGCTACTTCGCCCTTTTTCTAGGCACCTTCTTTTAAGGCGAGACCAtt                                                                                                                                    >  5:319194/1‑149 (MQ=255)
       gaCACATTTGGACACTTTCACGAACGCGCGCGAAGGGGTAGGCTGCGCGTTCATTTCGCCAGCACCTCAGAAAATGCTTCAACAATTCCTGCAGGATTTCGGCTACTTCGCCCTTTTTCTAGGCACCTTCTTTGAAGGCGAGACCATTc                                                                                                                                   <  1:64701/149‑1 (MQ=255)
            aTTTGGACACTTTCACGAACGCGCGCGAAGGGGTAGGCTGCGCGTTCATTTCGCCAGCACCTCAGAAAATGCTTCAACAATTCCTGCAGGATTTCGGCTACTTCGCCCTTTTTCTAGGCACCTTCTTTGAAGGCGAGACCATTCTGGTg                                                                                                                              >  2:20317/1‑149 (MQ=255)
                 gACACTTTCACGAACGCGCGCGAAGGGGTAGGCTGCGCGTTCATTTCGCCAGCACCTCAGAAAATGCTTCAACAATTCCTGCAGGATTTCGGCTACTTCGCCCTTTTTCTAGGCACCTTCTTTGAAGGCGAGACCATTCTGGTGCTTGc                                                                                                                         <  5:67637/149‑1 (MQ=255)
                      tttCACGAACGCGCGCGAAGGGGTAGGCTGCGCGTTCATTTCGCCAGCACCTCAGAAAATGCTTCAACAATTCCTGCAGGATTTCGGCTACTTCGCCCTTTTTCTAGGCACCTTCTTTGAAGGCGAGACCATTCTGGTGCTTGCGGGAt                                                                                                                    >  7:112323/1‑149 (MQ=255)
                       ttCACGAACGCGCGCGAAGGGGTAGGCTGCGCGTTCATTTCGCCAGCACCTCAGAAAATGCTTCAACAATTCCTGCAGGATTTCGGCTACTTCGCCCTTTTTCTAGGCACCTTCTTTGAAGGCGAGACCATTCTGGTGCTTGCGGGAtt                                                                                                                   >  8:311537/1‑149 (MQ=255)
                        tCACGAACGCGCGCGAAGGGGTAGGCTGCGCGTTCATTTCGCCAGCACCTCAGAAAATGCTTCAACAATTCCTGCAGGATTTCGGCTACTTCGCCCTTTTTCTAGGCACCTTCTTTGAAGGCGAGACCATTCTGGTGCTTGCGGGAt                                                                                                                    <  5:140679/147‑1 (MQ=255)
                          aCGAACGCGCGCGAAGGGGTAGGCTGCGCGTTCATTTCGCCAGCACCTCAGAAAATGCTTCAACAATTCCTGCAGGATTTCGGCTACTTCGCCCTTTTTCTAGGCACCTTCTTTGGAGGCGAGACCATTCTGGTGCTTGCGGGATTCCt                                                                                                                >  4:227704/1‑149 (MQ=255)
                          aCGAACGCGCGCGAAGGGGTAGGCTGCGCGTTCATTTCGCCAGCACCTCAGAAAATGCTTCAACAATTCCTGCAGGATTTCGGCTACTTCGCCCTTTTTCTAGGCACCTTCTTTGGAGGCGAGACCATTCTGGTGCTTGCGGGATTCCt                                                                                                                >  8:114985/1‑149 (MQ=255)
                           cGAACGCGCGCGAAGGGGTAGGCTGCGCGTTCATTTCGCCAGCACCTCAGAAAATGCTTCAACAATTCCTGCAGGATTTCGGCTACTTCGCCCTTTTTCTAGGCACCTTCTTTGGAGGCGAGACCATTCTGGTGCTTGCGGGATTCCtt                                                                                                               <  7:114985/149‑1 (MQ=255)
                           cGAACGCGCGCGAAGGGGTAGGCTGCGCGTTCATTTCGCCAGCACCTCAGAAAATGCTTCAACAATTCCTGCAGGATTTCGGCTACTTCGCCCTTTTTCTAGGCACCTTCTTTGGAGGCGAGACCATTCTGGTGCTTGCGGGATTCCtt                                                                                                               <  3:227704/149‑1 (MQ=255)
                            gAACGCGGGCGAAGGGTTAGGCTGCGCGTTCATTTCGCCAGCACCTCAGAAAATGCTTCAAGATTTCCTGCAGGATTTCGGCTACTTCGCCCTTTTTCTAGGCACCTTCTTTGAAGGCGAGACCATTCTGGTGCTTGCGGGATTCCTTg                                                                                                              <  6:319194/149‑1 (MQ=255)
                                  gcgcgAAGGGGTAGGCTGCGCGTTCATTTCGCCAGCACCTCAGAAAATGCTTCAACAATTCCTGCAGGATTTCGGCTACTTCGCCCTTTTTCTAGGCACCTTCTTTGAAGGCGAGACCATTCTGGTGCTTGCGGGATTCCTTGCGTTcc                                                                                                        >  5:104687/1‑149 (MQ=255)
                                       aaGGGGTAGGCTGCGCGTTCATTTCGCCAGCACCTCAGAAAATGCTTCAACAATTCCTGCAGGATTTCGGCTACTTCGCCCTTTTTCTAGGCACCTTCTTTGAAGGCGAGACCATTCTGGTGCTTGCGGGATTCCTTGCGTTCCGCGGt                                                                                                   <  8:112323/149‑1 (MQ=255)
                                            gTAGGCTGCGCGTTCATTTCGCCAGCACCTCAGAAAATGCTTCAACAATTCCTGCAGGATTTCGGCTACTTCGCCCTTTTTCTAGGCACCTTCTTTGAAGGCGAGACCATTCTGGTGCTTGCGGGATTCCTTGCGTTCCGCGGTTACAt                                                                                              >  4:250277/1‑149 (MQ=255)
                                             tAGGCTGCGCGTTCATTTCGCCAGCACCTCAGAAAATGCTTCAACAATTCCTGCCGGATTTCGGCTACTTCGCCCTTTTTCTAGGCACCTTCTTTGAAGGCGAGACCATTCTGGTGCTTGCGGGTTTCCTTGCGTTCCGCGGTTACATg                                                                                             <  8:160684/149‑1 (MQ=255)
                                              aGGCTGCGCGTTCATTTCGCCAGCACCTCAGAAAATGCTTCAACAATTCCTGCAGGATTTCGGCTACTTCGCCCTTTTTCTAGGCACCTTCTTTGAAGGCGAGACCATTCTGGTGCTTGCGGGATTCCTTGCGTTCCGCGGTTACATg                                                                                             >  4:167906/1‑148 (MQ=255)
                                               ggCTGCGCGTTCATTTCGCCAGCACCTCAGAAAATGCTTCAACAATTCCTGCAGGATTTCGGCTACTTCGCCCTTTTTCTAGGCACCTTCTTTGAAGGCGAGACCATTCTGGTGCTTGCGGGATTCCTTGCGTTCCGCGGTTACATgg                                                                                            <  6:104687/148‑1 (MQ=255)
                                                     gcgTTCATTTCGCCAGCACCTCAGAAAATGCTTCAACAATTCCTGCAGGATTTCGGCTACTTCGCCCTTTTTCTAGGCACCTTCTTTGAAGGCGAGACCATTCTGGTGCTTGCGGGATTCCTTGCGTTCCGCGGTTACATGGATATCaa                                                                                     >  6:134281/1‑149 (MQ=255)
                                                       gTTCATTTCGCCAGCACCTCAGAAAATGCTTCAACAATTCCTGCAGGATTTCGGCTACTTCGCCCTTTTTCTAGGCACCTTCTTTGAAGGCGAGACCATTCTGGTGCTTGCGGGATTCCTTGCGTTCCGCGGTTACATGGATATCAATc                                                                                   <  1:20317/149‑1 (MQ=255)
                                                           aTTTCGCCAGCACCTCAGAAAATGCTTCAACAATTCCTGCAGGATTTCGGCTACTTCGCCCTTTTTCTAGGCACCTTCTTTGAAGGCGAGACCATTCTGGTGCTTGCGGGATTCCTTGCGTTCCGCGGTTACATGGATATCAATCTGGt                                                                               >  7:158046/1‑149 (MQ=255)
                                                            tttCGCCAGCACCTCAGAAAATGCTTCAACAATTCCTGCAGGATTTCGGCTACTTCGCCCTTTTTCTAGGCACCTTCTTTGAAGGCGAGACCATTCTGGTGCTTGCGGGATTCCTTGCGTTCCGCGGTTACATGGat                                                                                          >  8:74202/1‑137 (MQ=255)
                                                            tttCGCCAGCACCTCAGAAAATGCTTCAACAATTCCTGCAGGATTTCGGCTACTTCGCCCTTTTTCTAGGCACCTTCTTTGAAGGCGAGACCATTCTGGTGCTTGCGGGATTCCTTGCGTTCCGCGGTTACATGGat                                                                                          <  7:74202/137‑1 (MQ=255)
                                                              tCGCCAGCACCTCAGAAAATGCTTCAACAATTCCTGCAGGATTTCGGCTACTTCGCCCTTTTTCTAGGCACCTTCTTTGAAGGCGAGACCATTCTGGTGCTTGCGGGATTCCTTGCGTTCCGCGGTTACATGGATATCAATCTGGTAt                                                                             <  3:250277/148‑1 (MQ=255)
                                                                  cAGCACCTCAGAAAATGCTTCAACAATTCCTGCAGGATTTCGGCTACTTCGCCCTTTTTCTAGGCACCTTCTTTGAAGGCGAGACCATTCTGGTGCTTGCGGGATTCCTTGCGTTCCGCGGTTACATGGATATCAATCTGGTATGCCtg                                                                        >  3:46026/1‑149 (MQ=255)
                                                                   aGCACCTCAGAAAATGCTTCAACAATTCCTGCAGGATTTCGGCTACTTCGCCCTTTTTCTAGGCACCTTCTTTGaa                                                                                                                                                >  6:186316/1‑76 (MQ=255)
                                                                   aGCACCTCAGAAAATGCTTCAACAATTCCTGCAGGATTTCGGCTACTTCGCCCTTTTTCTAGGCACCTTCTTTGaa                                                                                                                                                <  5:186316/76‑1 (MQ=255)
                                                                   aGCACCTCAGAAAATGCTTCAACAATTCCTGCAGGATTTCGGCTACTTCGCCCTTTTTCTAGGCACCTTCTTTGAAGGCGAGACCATTCTGGTGCTTGCGGGATTCCTTGCGTTCCGCGGTTACATGGATATCAATCTGGTATGCCtgg                                                                       >  7:155419/1‑149 (MQ=255)
                                                                        cTCAGAAAATGCTTCAACAATTCCTGCAGGATTTCGGCTACTTCGCCCTTTTTCTAGGCACCTTCTTTGAAGGCGAGACCATTCTGGTGCTTg                                                                                                                          >  6:220633/1‑93 (MQ=255)
                                                                        cTCAGAAAATGCTTCAACAATTCCTGCAGGATTTCGGCTACTTCGCCCTTTTTCTAGGCACCTTCTTTGAAGGCGAGACCATTCTGGTGCTTg                                                                                                                          <  5:220633/93‑1 (MQ=255)
                                                                         tCAGAAAATGCTTCAACAATTCCTGCAGGATTTCGGCTACTTCGCCCTTTTTCTAGGCACCTTCTTTGAAGGCGAGACCATTCTGGTGCTTGCGGGATTCCTTGCGTTCCGCGGTTACATGGATATCAATCt                                                                                  >  4:161748/1‑132 (MQ=255)
                                                                         tCAGAAAATGCTTCAACAATTCCTGCAGGATTTCGGCTACTTCGCCCTTTTTCTAGGCACCTTCTTTGAAGGCGAGACCATTCTGGTGCTTGCGGGATTCCTTGCGTTCCGCGGTTACATGGATATCAATCt                                                                                  <  6:237367/132‑1 (MQ=255)
                                                                         tCAGAAAATGCTTCAACAATTCCTGCAGGATTTCGGCTACTTCGCCCTTTTTCTAGGCACCTTCTTTGAAGGCGAGACCATTCTGGTGCTTGCGGGATTCCTTGCGTTCCGCGGTTACATGGATATCAATCt                                                                                  <  3:161748/132‑1 (MQ=255)
                                                                           aGAAAATGCTTCAACAATTCCTGCAGGATTTCGGCTACTTCGCCCTTTTTCTAGGCACCTTCTTTGAAGGCGAGACCATTCTGGTGCTTGCGGGATTCCTTGCGTTCCGCGGTTACATGGATATCAATCTGGTATGCCTGGTGGCcttc                                                               <  5:134281/149‑1 (MQ=255)
                                                                             aaaaTGCTTCAACAATTCCTGCAGGATTTCGGCTACTTCGCCCTTTTTCTAGGCACCTTCTTTGAAGGCGAGACCATTCTGGTGCTTGCGGGATTCCTTGCGTTCCGCGGTTACATGGATATCAATCTGGTATGCCtg                                                                        >  6:7742/1‑138 (MQ=255)
                                                                             aaaaTGCTTCAACAATTCCTGCAGGATTTCGGCTACTTCGCCCTTTTTCTAGGCACCTTCTTTGAAGGCGAGACCATTCTGGTGCTTGCGGGATTCCTTGCGTTCCGCGGTTACATGGATATCAATCTGGTATGCCtg                                                                        <  5:7742/138‑1 (MQ=255)
                                                                             aaaaTGCTTCAACAATTCCTGCAGGATTTCGGCTACTTCGCCCTTTTTCTAGGCACCTTCTTTGAAGGCGAGACCATTCTGGTGCTTGCGGGATTCCTTGCGTTCCGCGGTTACATGGATATCAATCTGGTATGCCTGGTGGCcttctt                                                             >  6:118895/1‑149 (MQ=255)
                                                                              aaaTGCTTCAACAATTCCTGCAGGATTTCGGCTACTTCGCCCTTTTTCTAGGCACCTTCTTTGAAGGCGAGACCATTCTGGTGCTTGCGGGATTCCTTGCGTTCCGCGGTTACATGGATATCAATCTGGTATGCCTGGTGGCcttctt                                                             <  7:311537/148‑1 (MQ=255)
                                                                                aTGCTTCAACAATTCCTGCAGGATTTCGGCTACTTCGCCCTTTTTCTAGGCACCTTCTTTGAAGGCGAGACCATTCTGGTGCTTGCGGGATTCCTTGCGTTCCGCGGTTACATGGATATCAATCTGGTATGCCTGGTGGCCTTCTTCgg                                                          <  4:46026/149‑1 (MQ=255)
                                                                                 tGCTTCAACAATTCCTGCAGGATTTCGGCTACTTCGCCCTTTTTCTAGGCACCTTCTTTGAAGGCGAGACCATTCTGGTGCTTGCGGGATTCCTTGCGTTCCGCGGTTACATGGATATCAATCTGGTATGc                                                                           >  7:1355/1‑131 (MQ=255)
                                                                                 tGCTTCAACAATTCCTGCAGGATTTCGGCTACTTCGCCCTTTTTCTAGGCACCTTCTTTGAAGGCGAGACCATTCTGGTGCTTGCGGGATTCCTTGCGTTCCGCGGTTACATGGATATCAATCTGGTATGc                                                                           <  8:6543/131‑1 (MQ=255)
                                                                                 tGCTTCAACAATTCCTGCAGGATTTCGGCTACTTCGCCCTTTTTCTAGGCACCTTCTTTGAAGGCGAGACCATTCTGGTGCTTGCGGGATTCCTTGCGTTCCGCGGTTACATGGATATCAATCTGGTATGc                                                                           >  7:6543/1‑131 (MQ=255)
                                                                                 tGCTTCAACAATTCCTGCAGGATTTCGGCTACTTCGCCCTTTTTCTAGGCACCTTCTTTGAAGGCGAGACCATTCTGGTGCTTGCGGGATTCCTTGCGTTCCGCGGTTACATGGATATCAATCTGGTATGc                                                                           <  8:1355/131‑1 (MQ=255)
                                                                                    ttCAACAATTCCTGCAGGATTTCGGCTACTTCGCCCTTTTTCTAGGCACCTTCTTTGAAGGCGAGACCATTCTGGTGCTTGCGGGATTCCTTGCGTTCCGCGGTTACATGGATATCAATCTGGTATGCCTGGTGACCTTCTTCGGCAGc                                                      >  2:282007/1‑149 (MQ=255)
                                                                                     tCAACAATTCCTGCAGGATTTCGGCTACTTCGCCCTTTTTCTAGGCACCTTCTTTGAAGGCGAGACCATTCTGGTGCTTGCGGGATTCCTTGCGTTCCGCGGTTACATGGATATCAATCTGGTATGCCTGGTGGCCTTCTTCGGCAGc                                                      >  4:115756/1‑148 (MQ=255)
                                                                                      caacaaTTCCTGCAGGATTTCGGCTACTTCGCCCTTTTTCTAGGCACCTTCTTTGAAGGCGAGACCATTCTGGTGCTTGCGGGATTCCTTGCGTTCCGCGTTTACATGGATATCAATCTGGTATTCCTGGTGGCCTTCTTCGGCAGCTa                                                    >  3:240311/1‑149 (MQ=255)
                                                                                              ccTGCAGGATTTCGGCTACTTCGCCCTTTTTCTAGGCACCTTCTTTGAAGGCGAGACCATTCTGGTGCTTGCGGGATTCCTTGCGTTCCGCGGTTACATGGATATCAATCTGGTATGCCTGGTGGCCTTCTTCGGCAGCTACGCGGGc                                             <  8:158046/148‑1 (MQ=255)
                                                                                              ccTGCAGGATTTCGGCTACTTCGCCCTTTTTCTAGGCACCTTCTTTGAAGGCGAGACCATTCTGGTGCTTGCGGGATTCCTTGCGTTCCGCGGTTACATGGATATCAATCTGGTATGCCTGGTGGCCTTCTTCGGCAGCTACGCGGGc                                             <  5:118895/148‑1 (MQ=255)
                                                                                              ccTGCAGGATTTCGGCTACTTCGCCCTTTTTCTAGGCACCTTCTTTGAAGGCGAGACCATTCTGGTGCTTGCGGGATTCCTTGCGTTCCGCGGTTACATGGATATCAATCTGGTATGCCTGGTGGCCTTCTTCGGCAGCTACGCGGGc                                             <  3:167906/148‑1 (MQ=255)
                                                                                                  cAGGATTTCGGCTACTTCGCCCTTTTTCTAGGCACCTTCTTTGAAGGCGAGACCATTCTGGTGCTTGCGGGATTCCTTGCGTTCCGCGGTTACATGGATATCAATCTGGTATGCCTGGTGGCCTTCTTCGGCAGCTACGCGGGCGAc                                          >  8:109202/1‑147 (MQ=255)
                                                                                                  cAGGATTTCGGCTACTTCGCCCTTTTTCTAGGCACCTTCTTTGAAGGCGAGACCATTCTGGTGCTTGCGGGATTCCTTGCGTTCCGCGGTTACATGGATATCAATCTGGTATGCCTGGTGGCCTTCTTCGGCAGCTACGCGGGCGACCa                                        <  3:115756/149‑1 (MQ=255)
                                                                                                     gATTTCGGCTACTTCGCCCTTTTTCTAGGCACCTTCTTTGAAGGCGAGACCATTCTGGTGCTTGCGGGATTCCTTGCGTTCCGCGGTTACATGGATATCAATCTGGTATGCCTGGTGGCCTTCTTCGGCAGCTACGCGGGCGACCAGtt                                     <  8:155419/149‑1 (MQ=255)
                                                                                                      aTTTCGGCTACTTCGCGCTTTTTCTAGGCACCTTCTTTGAAGGCGAGACCATTCTGGTGCTTGAGGGATTCCTTGCGTTCCGCGGTTACATGGATATCAATCTGGTATGCGTGGTGGCGTTCGTCGGCAGCTACGCGGGCGCCCAGTtg                                    >  8:94720/1‑149 (MQ=255)
                                                                                                      aTTTCGGCTACTTCGCCCTTTTTCTAGGCACCTTCTTTGAAGGCGAGACCATTCTGGTGCTTGCGGGATTCCTTGCGTTCCGCGGTTACATGGATATCAATCTGGTATGCCTGGTGGCCTTCTTCGGCAGCTACGCGGGCGACCAGTtg                                    >  4:244685/1‑149 (MQ=255)
                                                                                                       tttCGGCTACTTCGCCCTTTTTCTAGGCACCTTCTTTGAAGGCGAGACCATTCTGGTGCTTGCGGGATTCCTTGCGTTCCGCGGTTACATGGATATCAATCTGGTATGc                                                                           >  2:175467/1‑109 (MQ=255)
                                                                                                       tttCGGCTACTTCGCCCTTTTTCTAGGCACCTTCTTTGAAGGCGAGACCATTCTGGTGCTTGCGGGATTCCTTGCGTTCCGCGGTTACATGGATATCAATCTGGTATGc                                                                           <  1:175467/109‑1 (MQ=255)
                                                                                                           ggCTACTTCGCCCTTTTTCTAGGCACCTTCTTTGAAGGCGAGACCATTCTGGTGCTTGCGGGATTCCTTGCGTTCCGCGGTTACATGGATATCAATCTGGTATGCCTGGTGGCCTTCTTCGGCAGCTACGCGGGCGACCAGtt                                     >  1:121650/1‑143 (MQ=255)
                                                                                                           ggCTACTTCGCCCTTTTTCTAGGCACCTTCTTTGAAGGCGAGACCATTCTGGTGCTTGCGGGATTCCTTGCGTTCCGCGGTTACATGGATATCAATCTGGTATGCCTGGTGGCCTTCTTCGGCAGCTACGCGGGCGACCAGtt                                     <  2:121650/143‑1 (MQ=255)
                                                                                                              tACTTCGCCCTTTTTCTAGGCACCTTCTTTGAAGGCGAGACCATTCTGGTGCTTGCGGGATTCCTTGCGTTCCGCGGTTACATGGATATCAATCTGGTATGCCTGGTGGCCTTCTTCGGCAGCTACGCGGGCGACCAGTTGTGGTACt                             >  1:114495/1‑148 (MQ=255)
                                                                                                                cTTCGCCCTTTTTCTAGGCACCTTCTTTGAAGGCGAGACCATTCTGGTGCTTGCGGGATTCCTTGCGTTCCGCGGTTACATGGATATCAATCTGGTATGCCTGGTGGCCTTCTTCGGCAGCTACGCGGGCGACCAGTTGTGGTACTTc                           <  7:109202/148‑1 (MQ=255)
                                                                                                                 ttctatcTTTTTATAGTCAGCTTCTTTGAATGCGAGACCATTCTGGTGCTTGTGGGATTCCTTTCGTTCCGCTGTTACATGGATTTGAATCTGTTATGCCTGGTGTCCTTCTTCGGGAGCTACGAGGGCGTCCAGTTGTGGTACTTCAt                         <  4:240311/143‑1 (MQ=255)
                                                                                                                   cGCCCTTTTTCTAGGCACCTTCTTTGAAGGCGAGACCATTCTGGTGCTTGCGGGATTCCTTGCGTTCCGCGGTTACATGGATATCAATCTGGTATGCCTGGTGACCTTCTTCGGCAGCTACGCGGGCGACCAGTTGTGGTACTTCATg                        <  1:282007/148‑1 (MQ=255)
                                                                                                                      ccTTTTTCTAGGCACCTTCTTTGAAGGCGAGACCATTCTGGTGCTTGCGGGATTCCTTGCGTTCCGCGGTTACATGGATATCAATCTGGTATGCCTGGTGGCCTTCTTCGGCAGCTACGCGGGCGACCAGTTGTGGTACTTCATGGGcc                    >  4:111228/1‑149 (MQ=255)
                                                                                                                       cTTTTTCTAGGCACCTTCTTTGAAGGCGAGACCATTCTGGTGCTTGCGGGATTCCTTGCGTTCCGCGGTTACATGGATATCAATCTGGTATGCCTGGTGGCCTTCTTCGGCAGCTACGCgg                                               >  7:55412/1‑121 (MQ=255)
                                                                                                                       cTTTTTCTAGGCACCTTCTTTGAAGGCGAGACCATTCTGGTGCTTGCGGGATTCCTTGCGTTCCGCGGTTACATGGATATCAATCTGGTATGCCTGGTGGCCTTCTTCGGCAGCTACGCgg                                               <  8:55412/121‑1 (MQ=255)
                                                                                                                              tAGGCACCTTCTTTGAAGGCGAGACCATTCTGGTGCTTGCGGGATTCCTTGCGTTCCGCGGTTACATGGATATCAATCTGGTATGCCTGGTGGCCTTCTTCGGCAGCTACGCGGGCGACCAGTtgt                                   >  4:143232/1‑126 (MQ=255)
                                                                                                                              tAGGCACCTTCTTTGAAGGCGAGACCATTCTGGTGCTTGCGGGATTCCTTGCGTTCCGCGGTTACATGGATATCAATCTGGTATGCCTGGTGGCCTTCTTCGGCAGCTACGCGGGCGACCAGTtgt                                   <  3:143232/126‑1 (MQ=255)
                                                                                                                                   aCCTTCTTTGAAGGCGAGACCATTCTGGTGCTTGCGGGATTCCTTGCGTTCCGCGGTTACATGGATATCAATCTGGTATGCCTGGTGGCCTTCTTCGGCAGCTACGCGGGc                                             >  8:206919/1‑111 (MQ=255)
                                                                                                                                       tcttTGAAGGCGAGACCATTCTGGTGCTTGCGGGATTCCTTGCGTTCCGCGGTTACATGGATATCAATCTGGTATGCCTGGTGGCCTTCTTCGGCAGCTACGCGGGCGACCAGTtgtg                                  <  2:292888/118‑1 (MQ=255)
                                                                                                                                          ttGAAGGCGAGACCATTCTGGTGCTTGCGGGATTCCTTGCGTTCCGCGGTTACATGGATATCAATCTGGTATGCCTGGTGGCCTTCTTCGGCAGCTACGCGGGCGACCAGTTGTGGTACTTCATgg                       <  3:338612/126‑1 (MQ=255)
                                                                                                                                          ttGAAGGCGAGACCATTCTGGTGCTTGCGGGATTCCTTGCGTTCCGCGGTTACATGGATATCAATCTGGTATGCCTGGTGGCCTTCTTCGGCAGCTACGCGGGCGACCAGTTGTGGTACTTCATgg                       >  4:338612/1‑126 (MQ=255)
                                                                                                                                           tGAAGGCGAGACCATTCTGGTGCTTGCGGGATTCCTTGCGTTCCGCGGTTACATGGATATCAATCTGGTATGCCTGGTGGCCTTCTTCGGCAGCTACGCGGGCGACCAGTTGTGGTACTTCATGGGCCGACGCCACGGGCGCAGGa  <  2:114495/146‑1 (MQ=255)
                                                                                                                                             |                                                                                                                                               
ACGTCGAGACACATTTGGACACTTTCACGAACGCGCGCGAAGGGGTAGGCTGCGCGTTCATTTCGCCAGCACCTCAGAAAATGCTTCAACAATTCCTGCAGGATTTCGGCTACTTCGCCCTTTTTCTAGGCACCTTCTTTGAAGGCGAGACCATTCTGGTGCTTGCGGGATTCCTTGCGTTCCGCGGTTACATGGATATCAATCTGGTATGCCTGGTGGCCTTCTTCGGCAGCTACGCGGGCGACCAGTTGTGGTACTTCATGGGCCGACGCCACGGGCGCAGGA  >  NC_002947/5953150‑5953434

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑