| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 546,002 | G→A | 15.0% | intergenic (+42/‑31) | rpoC → / → PP_mr08 | DNA‑directed RNA polymerase subunit beta'/rpsL_psuedo ncRNA |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 546,002 | 0 | G | A | 15.0% | 140.0 / 14.9 | 60 | intergenic (+42/‑31) | rpoC/PP_mr08 | DNA‑directed RNA polymerase subunit beta'/rpsL_psuedo ncRNA |
| Reads supporting (aligned to +/- strand): ref base G (26/25); new base A (5/4); total (31/29) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.49e-01 | |||||||||||
CAGCGAGCGCAAGCGTCGCCGTGATGCCGACAAACCGCTGCGTGTAAGCGCCAGTGAAGTGGAAGCTGCACTGACCGAAGCGCTGAATTCCAGCGGTAATTAAGTACAGGGCAAGGCCCCGGCAAGCCTCGGGCGATCATCGGCGACATGAAATGTTGCCGATGATCGGGCGGGGAGGGCCGGGGCCTCGTCTTGACTGGGTGCAAGATCCTCTTTAGACTTTTGTACCCTTAAATTTGGTGAGGCTCCGTCTCGCCAATTTTTGGCTTTCTTGCAAGACAATAGAGTC > NC_002947/545858‑546146 | caGCGAGCGCAAGCGTCGCCGTGATGCCGACAAACCGCTGCGTGTAAGCGCCAGTGAAGTGGAAGCTGCACTGACCGCAGCGCTGAATTCCAGCGGTAATTAAGTACAGGGCAAGGCCCCGGCAAGCCTCGGGCGATCATCGGCGACAt > 7:302053/1‑149 (MQ=255)caGCGAGCGCAAGCGTCGCCGTGATGCCGACAAACCGCTGCGTGTAAGCGCCAGTGAAGTGGAAGCTGCACTGACCGAAGCGCTGAATTCCAGCGGTAATTAAGTACAGGGCAAGGCCCCGGCAAGCCTCGGGCGATCATCGGCGACAt < 3:15457/149‑1 (MQ=255) gcgagcgCAAGCGTCGCCGTGATGCCGACAAACCGCTGCGTGTAAGCGCCAGTGAAGTGGAAGCTGCACTGACCGAAGCGCTGAATTCCAGCGGTAATTAAGTACAGGGCAAGGCCCCGGCAAGCCTCGGGCGATCATCGGCGACATg > 7:199751/1‑148 (MQ=255) gcAAGCGTCGCCGTGATGCCGACAAACCGCTGCGTGTAAGCGCCAGTGAAGTGGAAGCTGCACTGACCGAAGCGCTGAATTCCAGCGGTAATTAAGTACAGGGCAAGGCCCCGGCAAGCCTCGGGCGATCATCGGCGACATGAAATGtt > 4:63000/1‑149 (MQ=255) gcAAGCGTCGCCGTGATGCCGACAAACCGCTGCGTGTAAGCGCCAGTGAAGTGGAAGCTGCACTGACCGAAGCGCTGAATTCCAGCGGTAATTAAGTACAGGGCAAGGCCCCGGCAAGCCTCGGGCGATCATCGGCGACATGAAATGtt < 3:63000/149‑1 (MQ=255) cGTCGCCGTGATGCCGACAAACCGCTGCGTGTAAGCGCCAGTGAAGTGGAAGCTGCACTGACCGAAGCGCTGAATTCCAGCGGTAATTAAGTACAGGGCAAGGCCCCGGCAAGCCTCGGGCGATCATCGGCGACATGAAATGTTGCCga < 8:40913/149‑1 (MQ=255) gCCGTGATGCCGACAAACCGCTGCGTGTAAGCGCCAGTGAAGTGGAAGCTGCACTGACCGAAGCGCTGAATTCCAGCGGTAATTAAGTACAGGGCAAGGCCCCGGCAAGCCTCGGGCGATCATCGGCGACATGAAATGTTGCCgatgat > 8:110174/1‑149 (MQ=255) ccGTGATGCCGACAAACCGCTGCGAGTAAGCGCCAGTGAAGTGGAAGCTGCACTGACCGAAGCGCTGAATTCCAGCGGTAATTAAGTACAGGGCAAGGCCCCGGCAAGCCTCGGGCGATCATCGGCGACATGAAATGTTGCCGATGATc < 6:358010/149‑1 (MQ=255) gTGATGCCGACAAACCGCTGCGTGTAAGCGCCAGTGAAGTGGAAGCTGCACTGACCGAAGCGCTGAATTCCAGCGGTAATTAAGTACAGGGCAAGGCCCCGGCCCTCCCCGCCCGATCATCGGCAACATTTCATGTCGCCGATGATcgc < 2:56231/149‑2 (MQ=25) gACAAACCGCTGCGTGTAAGCGCCAGTGAAGTGGAAGCTGCACTGACCGAAGCGCTGAATTCCAGCGGTAATTAAGTACAGGGCAAGGCCCCGGCAAGCCTCGGGCGATCATCGGCAACATTTCATGTCGCCga < 1:337291/134‑1 (MQ=255) gACAAACCGCTGCGTGTAAGCGCCAGTGAAGTGGAAGCTGCACTGACCGAAGCGCTGAATTCCAGCGGTAATTAAGTACAGGGCAAGGCCCCGGCAAGCCTCGGGCGATCATCGGCAACATTTCATGTCGCCga > 2:337291/1‑134 (MQ=255) aCAAACCGCTGCGTGTAAGCGCCAGTGAAGTGGAAGCTGCACTGACCGAAGCGCTGAATTCCAGCGGTAATTAAGTACAGGGCAAGGCCCCGGCAAGCCTCGGGCGATCATCGGCGACATGAAATGTTGCCGATGATCGGGCGGGGAgg < 8:199751/149‑1 (MQ=255) aaaCCGCTGCGTGTAAGCGCCAGTGAAGTGGAAGCTGCACTGACCGAAGCGCTGAATTCCAGCGGTAATTAAGTACAGGGCAAGGCCCCGGCAAGCCTCGGGCGATCATCGGCGACATGAAATGTTGCCGATGATCGGGCGGGGAGGGc > 8:177341/1‑149 (MQ=255) gCTGCGTGTAAGCGCCAGTGAAGTGGAAGCTGCACTGACCGAAGCGCTGAATTCCAGCGGTAATTAAGTACAGGGCAAGGCCCCGGCCCTCCCCGCCCGATCATCGGCAACATTTCATGTCGCCgat > 4:214706/1‑127 (MQ=21) gCTGCGTGTAAGCGCCAGTGAAGTGGAAGCTGCACTGACCGAAGCGCTGAATTCCAGCGGTAATTAAGTACAGGGCAAGGCCCCGGCCCTCCCCGCCCGATCATCGGCAACATTTCATGTCGCCgat < 3:214706/127‑1 (MQ=21) tgtAAGCGCCAGTGAAGTGGAAGCTGCACTGACCGAAGCGCTGAATTCCAGCGGTAATTAAGTACAGGGCAAGGCCCCGGCAAGCCTCGGGCGATCATCGGCGACATGAAATGTTGCCGATGATCGGGCGGGGAGGGCCGGGGCCTCGt > 4:302427/1‑149 (MQ=255) gtAAGCGCCAGTGAAGTGGAAGCTGCACTGACCGAAGCGCTGAATTCCAGCGGTAATTAAGTACAGGGCAAGGCCCCGGCAAGCCTCGGGCGATCATCGGCGACATGAAATGTTGCCGATGATCGGGCGGGGAGGGCCggg < 5:183300/141‑1 (MQ=255) gtAAGCGCCAGTGAAGTGGAAGCTGCACTGACCGAAGCGCTGAATTCCAGCGGTAATTAAGTACAGGGCAAGGCCCCGGCAAGCCTCGGGCGATCATCGGCGACATGAAATGTTGCCGATGATCGGGCGGGGAGGGCCggg > 6:183300/1‑141 (MQ=255) aGCGCCAGTGAAGTGGAAGCTGCACTGACCGAAGCGCTGAATTCCAGCGGTAATTAAGTACAGGGCAAGGCCCCGGCAAGCCTCGGGCGATCATCGGCGACATGAAATGTTGCCGATGATCGGGCGGGGAGGGCCGGGGCCTCGTCtt < 7:110174/148‑1 (MQ=255) cAGTGAAGTGGAAGCTGCACTGACCGAAGCGCTGAATTCCAGCGGTAATTAAGTACAGGGCAAGGCCCCGGCAAGCCTCGGGCGATCATCGGCGACATGAAATGTTGCCGATGATCGGGCGGGGAGGGCCg > 3:3655/1‑131 (MQ=255) gTGGAAGCTGCACTGACCGAAGCGCTGAATTCCAGCGGTAATTAAGTACAGGGCAAGGCCCCGGCAAGCCTCGGGCGATCATCGGCGACATGAAATGTTGCCGATGATCGGGCGGGGAGGGCCGGGGCCTCGTc < 3:226647/134‑1 (MQ=255) gTGGAAGCTGCACTGACCGAAGCGCTGAATTCCAGCGGTAATTAAGTACAGGGCAAGGCCCCGGCAAGCCTCGGGCGATCATCGGCGACATGAAATGTTGCCGATGATCGGGCGGGGAGGGCCGGGGCCTCGTc > 4:226647/1‑134 (MQ=255) tGGAAGCTGCACTGACCGAAGCGCTGAATTCCAGCGGTAATTAAGTACAGGGCAAGGCCCCGGCAAGCCTCGGGCGATCATCGGCGACATGAAATGTTGCCGATGATCGGGCGGGGAGGGCCGGGGCCTCGTCTTGACTGGGTGc > 4:258052/1‑145 (MQ=255) tGGAAGCTGCACTGACCGAAGCGCTGAATTCCAGCGGTAATTAAGTACAGGGCAAGGCCCCGGCAAGCCTCGGGCGATCATCGGCGACATGAAATGTTGCCGATGATCGGGCGGGGAGGGCCGGGGCCTCGTCTTGACTGGGTGCAAGa < 7:177341/149‑1 (MQ=255) tGCACTGACCGAAGCGCTGAATTCCAGCGGTAATTAAGTACAGGGCAAGGCCCCGGCAAGCCTCGGGCGATCATCGGCGACATGAAATGTTGCCGATGATCGGGCGGGGAGGGCCGGGGCCTCGTCTTGACTGGGTGCAAGATCCTCtt > 2:106101/1‑149 (MQ=255) gCACTGACCGAAGCGCTGAATTCCAGCGGTAATTAAGTACAGGGCAAGGCCCCGGCAAGCCTCGGGCGATCATCGGCGACATGAAATGTTGCCGATGATCGGGCGGGGAGGGCCGGGGCCTCGTCTTGACTGGGTGCAAGATCCTCtt > 3:25222/1‑148 (MQ=255) cACTGACCGAAGCGCTGAATTCCAGCGGTAATTAAGTACAGGGCAAGGCCCCGGCAAGCCTCGGGCGATCATCGGCGACATGAAATGTTGCCGATGATCGGGCGGGGAGGGCCGGGGCCTCGTCTTGACTGGGTGCAAGATCCTCTTTa < 1:106101/149‑1 (MQ=255) aCTGACCGAAGCGCTGAATTCCAGCGGTAATTAAGTACAGGGCAAGGCCCCGGCAAGCCTCGGGCGATCATCGGCGACATGAAATGTTGCCGATGATCGGGCGGGGAGGGCCGGGGCCTCGTCTTGACTGGGTGCAAGATCCTCtt > 1:213565/1‑146 (MQ=255) gcTGAATTCCAGCGGTAATTAAGTACAGGGCAAGGCCCCGGCAAGCCTCGGGCGATCATCGGCGACATGAAATGTTGCCGATGATCGGGCGGGGAGGGCCGGGGCCTCGTCTTGACTGGGTGCAAGATCCTCTTTAGACTTTTGTAcc < 6:297114/148‑1 (MQ=255) gcTGAATTCCAGCGGTAATTAAGTACAGGGCAAGGCCCCGGCAAGCCTCGGGCGATCATCGGCGACATGAAATGTTGCCGATGATCGGGCGGGGAGGGCCGGGGCCTCGTCTTGACTGGGTGCAAGATCCTCTTTAGACTTTTGTAcc > 5:297114/1‑148 (MQ=255) gAATTCCAGCGGTAATTAAGTACAGGGCAAGGCCCCGGCAAGCCTCGGGCGATCATCGGCGACATGAAATGTTGCCGATGATCGGGCGGGGAGGGCCGGGGCCTCGTCTTGACTGGGTGCAAGATCCTCttt > 8:10855/1‑132 (MQ=255) gAATTCCAGCGGTAATTAAGTACAGGGCAAGGCCCCGGCAAGCCTCGGGCGATCATCGGCGACATGAAATGTTGCCGATGATCGGGCGGGGAGGGCCGGGGCCTCGTCTTGACTGGGTGCAAGATCCTCttt < 7:10855/132‑1 (MQ=255) ccAGCGGTAATTAAGTACAGGGCAAGGCCCCGGCAAGCCTCGGGCGATCATCGGCGACATGAAATGTTGCCGATGATCGGGCGGGGAGGGCCGGGGCCTCGTCTTGACTGGGTGCAAGATCCTCTTTAGACTTTTGTACCCTTAAAttt > 4:67772/1‑149 (MQ=255) cAGCGGTAATTACGTACAGGGCAAGGCCCCGGCAAGCCTCGGGCGATCATCGGCGACATGAAATGTTGCCGATGATCGGGCGGGGAGGGCCGGGGCCTCGTCTTGACTGGGTGCAAGATCCTCTTTAGACTTTTGTACCCTTAAATTTg < 8:302053/149‑1 (MQ=255) cAGCGGTAATTAAGTACAGGGCAAGGCCCCGGCAAGCCTCGGGCGATCATCGGCGACATGAAATGTTGCCGATGATCGGGCGGGGAGGGCCGGGGCCTCGTCTTGACTGGGTGCAAGATCCTCTTTAGACTTTTGTACCCTTAAATTTg > 1:319407/1‑149 (MQ=255) gCGGTAATTAAGTACAGGGCAAGGCCCCGGCAAGCCTCGGGCGATCATCGGCGACATGAAATGTTGCCGATGATCGGGCGGGGAGGGCCGGGGCCTCGTCTTGACTGGGTGCAAGATCCTCTTTAGACTTTTGTACCCTTAAATTTGGt < 3:67772/149‑1 (MQ=255) gCGGTAATTAAGTACAGGGCAAGGCCCCGGCAAGCCTCGGGCGATCATCGGCGACATGAAATGTTGCCGATGATCGGGCGGGGAGGGCCGGGGCCTCGTCTTGACTGGGTGCAAGATCCTCTTTAGACTTTTGTACCCTTAAATTTGGt > 8:202025/1‑149 (MQ=255) gTAATTAAGTACAGGGCAAGGCCCCGGCAAGCCTCGGGCGATCATCGGCGACATGAAATGTTGCCGATGATCGGGCGGGGAGGGCCGGGGCCTCGTCTTGACTGGGTGCAAGATCCTCtt > 7:329949/1‑120 (MQ=255) gTAATTAAGTACAGGGCAAGGCCCCGGCAAGCCTCGGGCGATCATCGGCGACATGAAATGTTGCCGATGATCGGGCGGGGAGGGCCGGGGCCTCGTCTTGACTGGGTGCAAGATCCTCtt < 8:329949/120‑1 (MQ=255) tAATTAAGTACAGGGCAAGGCCCCGGCAAGCCTCGGGCGATCATCGGCGACATGAAATGTTGCCGATGATCGGGCGGGGAGGGCCGGGGCCTCGTCTTGACTGGGTGCAAGATCCTCTTTAGACTTTTGTACCCTTAAATTTGGTGAgg < 6:173598/149‑1 (MQ=255) ttAAGTACAGGGCAAGGCCCCGGCAAGCCTCGGGCGATCATCGGCGACATGAAATGTTGCCGATGATCGGGCGGGGAGGGCCGGGGCCTCGTCTTGACTGGGTGCAAGATACTCTTTAGACTTTTGTACCCTTAAATTTGGTGAGGCTc > 4:89992/1‑149 (MQ=255) tACAGGGCAAGGCCCCGGCAAGCCTCGGGCGATCATCGGCGACATGAAATGTTGCCGATGATCGGGCGGGGAGGGCCGGGGCCTCGTCTTGACTGGGTGCAAGATCCTCTTTAGACTTTTGTACCCTTAAATTTGGTGAGGCTCCGtct > 2:307686/1‑149 (MQ=255) gacgaGGCCCCGGCCCTCCCCGCCCGATCATCGGCAACATTTAATGTCGCCGATGATCGCCCGAGGCTTGACGGGGCCTCGTCTTGACTGGGTGCAAGATCCTCTTTAGACTTTTGTACCCTTAAATTTGGTGAGGCTCCGTCTCGcc > 4:322464/5‑148 (MQ=14) gCCGCGGCAAGCCTCGGGCTATCATCGGCGACATGAAATGTTGCCGATGATCGGGCGGGGAGGGCCGGGGCCTCGTCTTGACTGGGTGCAAGATCCTCTTTAGACTTTTGTACCCTTAAATTTGGTGAGGCTCCGTCTCGCCAAttttt < 7:202025/149‑1 (MQ=255) ccccGGCAAGCCTCGGGCGATCATCGGCGACATGAAATGTTGCCGATGATCGGGCGGGGAGGGCCGGGGCCTCGTCTTGACTGGGTGCAAGATCCTCTTTAGACTTTTGTACCCTTAAATTTGGTGAGGCTCCGTCTCGCCaa < 2:7566/143‑1 (MQ=255) ccccGGCAAGCCTCGGGCGATCATCGGCGACATGAAATGTTGCCGATGATCGGGCGGGGAGGGCCGGGGCCTCGTCTTGACTGGGTGCAAGATCCTCTTTAGACTTTTGTACCCTTAAATTTGGTGAGGCTCCGTCTCGCCaa > 1:7566/1‑143 (MQ=255) ccGGCAAGCCTCGGGCGATCATCGGCGACATGAAATGTTGCCGATGATCGGGCGGGGAGGGCCGGGGCCTCGTCTTGACTGGGTGCAAGATCCTCTTTAGACTTTTGTACCCTTAAATTTGGTGAGGCTCCGTCTCGCCAATTTTTGGc > 4:162227/1‑149 (MQ=255) aaGCCTCGGGCGATCATCGGCGACATGAAATGTTGCCGATGATCGGGCGGGGAGGGCCGGGGCCTCGTCTTGACTGGGTGCAAGATCCTCTTTAGACTTTTGTACCCTTAAATTTGGTGAGGCTCCGTCTCGCCAATTTTTGGCTTTCt > 1:57313/1‑149 (MQ=255) aGCCTCGGGCGATCATCGGCGACATGAAATGTTGCCGATGATCGGGCGGGGAGGGCCGGGGCCTCGTCTTGACTGGGTGCAAGATCCTCTTTAGACTTTTGTACCCTTAAATTTGGTGAGGCTCCGTCTCGCCAATTTTTGGCTTTCtt < 3:162227/149‑1 (MQ=255) gtCGGGCGATCATCGGCGACATGAAATGTTGCCGATGATCGGGCGGGGAGTGCCGGGGCCTCGTCTTGACTGGGTGCAAGATCCTCTTTAGACTTTTGTACCCTTAAATTTGGGGAGGCTCCGTCTCGCCAATTTTTGGCTTTCTTGCa < 2:213565/148‑1 (MQ=255) gCGATCATCGGCGACATGAAATGTTGCCGATGATCGGGCGGGGAGGGCCGGGGCCTCGTCTTGACTGGGTGCAAGATCCTCTTTAGACTTTTGTACCCTTAAATTTGGTGAGGCTCCGTCTCGCCAATTTTTGGCTTTCTTGCAAGa < 2:98836/147‑1 (MQ=255) gCGATCATCGGCGACATGAAATGTTGCCGATGATCGGGCGGGGAGGGCCGGGGCCTCGTCTTGACTGGGTGCAAGATCCTCTTTAGACTTTTGTACCCTTAAATTTGGTGAGGCTCCGTCTCGCCAATTTTTGGCTTTCTTGCAAGa > 1:98836/1‑147 (MQ=255) gCGATCATCGGCGACATGAAATGTTGCCGATGATCGGGCGGGGAGGGCCGGGGCCTCGTCTTGACTGGGTGCAAGATCCTCTTTAGACTTTTGTACCCTTAAATTTGGTGAGGCTCCGTCTCGCCAATTTTTGGCTTTCTTGCAAGa < 2:102807/147‑1 (MQ=255) gCGATCATCGGCGACATGAAATGTTGCCGATGATCGGGCGGGGAGGGCCGGGGCCTCGTCTTGACTGGGTGCAAGATCCTCTTTAGACTTTTGTACCCTTAAATTTGGTGAGGCTCCGTCTCGCCAATTTTTGGCTTTCTTGCAAGa > 1:102807/1‑147 (MQ=255) ccGATCATCGGCAACATTTCATGTCGCCGATGATCGGGCGGGGAGGGCCGGGGCCTCGTCTTGACTGGGTGCAAGATCCTCTTTAGACTTTTGTACCCTTAAATTTGGTGAGGCTCCGTCTCGCCAAttttt > 6:211163/2‑132 (MQ=38) ccGATCATCGGCAACATTTCATGTCGCCGATGATCGGGCGGGGAGGGCCGGGGCCTCGTCTTGACTGGGTGCAAGATCCTCTTTAGACTTTTGTACCCTTAAATTTGGTGAGGCTCCGTCTCGCCAAttttt < 5:211163/131‑1 (MQ=38) gATCATCGGCGACATGAAATGTTGCCGATGATCGGGCGGGGAGGGCCGGGGCCTCGTCTTGACTGGGTGCAAGATCCTCTTTAGACTTTTGTACCCTTAAATTTGGTGAGGCTCCGTCTCGCCAATTTTTgg < 8:175502/132‑1 (MQ=255) atcatcGGCGACATGAAATGTTGCCGATGATCGGGCGGGGAGGGCCGGGGCCTCGTCTTGACTGGGTGCAAGATCCTCTTTAGACTTTTGTACCCTTAAATTTGGTGAGGCTCCGTCTCGCCAATTTTTGGCTTTCTTGCAAGACAATa < 1:307686/149‑1 (MQ=255) tcGGCAACATTTCATGTCGCCGATGATCGGGCGGGGAGGGCCGGGGCCTCGTCTTGACTGGGTGCAAGATCCTCTTTAGACTTTTGTACCCTTAAATTTGGTGAGGCTCCGTCTCGCCAATTTTTGGCTTTCTTGCAAGACAATAGAGt > 6:137106/1‑149 (MQ=255) ggCGACATGAAATGTTGCCGATGATCGGGCGGGGAGGGCCGGGGCCTCGTCTTGACTGGGTGCAAGATCCTCTTTAGACTTTTGTACCCTTAAATTTGGTGAGGCTCCGTCTCGCCAATTTTTGGCTTTCTTGCAAGACAATAGAGTc < 2:319407/148‑1 (MQ=255) | CAGCGAGCGCAAGCGTCGCCGTGATGCCGACAAACCGCTGCGTGTAAGCGCCAGTGAAGTGGAAGCTGCACTGACCGAAGCGCTGAATTCCAGCGGTAATTAAGTACAGGGCAAGGCCCCGGCAAGCCTCGGGCGATCATCGGCGACATGAAATGTTGCCGATGATCGGGCGGGGAGGGCCGGGGCCTCGTCTTGACTGGGTGCAAGATCCTCTTTAGACTTTTGTACCCTTAAATTTGGTGAGGCTCCGTCTCGCCAATTTTTGGCTTTCTTGCAAGACAATAGAGTC > NC_002947/545858‑546146 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |