Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	100,117	T→G	5.1%	N98H (AAC→CAC) ‡	PP_0094 ←	5'‑nucleotidase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	100,117	0	T	G	5.1%	245.3 / 4.1	78	N98H (AAC→CAC) ‡	PP_0094	5'‑nucleotidase
Reads supporting (aligned to +/- strand):  ref base T (36/38);  new base G (2/2);  total (38/40)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.06e-01

GCTCGACCTTGTTGGTGCGCGTGCCGTCCAGTTCACTGCCGTAGATCACCTTGAAGTGATGATCGAACGCAAAGTGCCGAGCGATTTCGCGGGCGAACTCCCATGGTTTGGAGGTGGCAATGTACAGCGTGCGGCCCTGCCCGTTCAGGGCCTCAA‑GCAACTCCGGCAC‑ACCTTCGAACACGAGGTTTTCGTAAAGACCGGTCACGCGGAAGCGCTCGCGGTAGAAGTTCACCGCATCCCAGGCCTTGGCTTCGTCGAAGCTGTAGAACTGCATGAAAGCCTGCAGC  >  NC_002947/99973‑100259                                                                                                                                                 |                                                                                                                                                 gCTCGACCTTGTTGGTGCGCGTGCCGTCCAGTTCACTGCCGTAGATCACCTTGAAGTGATGATCGAACGCAAAGTGCCGAGCGATTTCGCGGGCGAACTCCCATGGTTTGGAGGTGGCAATGTACAGCGTGCGGCCCTGCCCGTTCAgg                                                                                                                                              <  5:360620/149‑1 (MQ=255) gCTCGACCTTGTTGGTGCGCGTGCCGTCCAGTTCACTGCCGTAGATCACCTTGAAGTGATGATCGAACGCAAAGTGCCGAGCGATTTCGCGGGCGAACTCCCATGGTTTGGAGGTGGCAATGTACAGCGTGCGGCCCTGCCCGTTCAgg                                                                                                                                              <  1:346216/149‑1 (MQ=255) gCTCGACCTTGTTGGTGCGCGTGCCGTCCAGTTCACTGCCGTAGATCACCTTGAAGTGATGATCGAACGCAAAGTGCCGAGCGATTTCGCGGGCGAACTCCCATGGTTTGGAGGTGGCAATGTACAGCGTGCGGCCCTGCCCGTTCAgg                                                                                                                                              <  3:216459/149‑1 (MQ=255) gCTCGACCTTGTTGGTGCGCGGGCCGTCCAGTTCACTGCCGTAGATCACCTTGAAGTGATGATCGAACGCAAAGTGCCGAGCGATTTCGCGGGCGAACTCCCATGGTTTGGAGGTGGCAATGTACAGCGTGCGGCCCTGCCCGTTCAgg                                                                                                                                              <  3:3838/149‑1 (MQ=255)  cTCGACCTTGTTGGTGCGCGTGCCGTCCAGTTCACTGCCGTAGATCACCTTGAAGTGATGATCGAACGCAAAGTGCCGAGCGATTTCGCGGGCGAACTCCCATGGTTTGGAGGTGGCAATGTACAGCGTGCGGCCCTGCCCGTTCAggg                                                                                                                                             >  8:170232/1‑149 (MQ=255)     gACCTTGTTGGTGCGCGTGCCGTCCAGTTCACTGCCGTAGATCACCTTGAAGTGATGATCGAACGCAAAGTGCCGAGCGATTTCGCGGGCGAACTCCCATGGTTTGGGGGTGGCAATGTACAGCGTGCGGCCCTGCCCGTTCAGGGCCt                                                                                                                                          >  2:26581/1‑149 (MQ=255)     gACCTTGTTGGTGCGCGTGCCGTCCAGTTCACTGCCGTAGATCACCTTGAAGTGATGATCGAACGCAAAGTGCCGAGCGATTTCGCGGGCGAACTCCCATGGTTTGGAGGTGGCAATGTACAGCGTGCGGCCCTGCCCGTTCAGGGc                                                                                                                                            <  2:165238/147‑1 (MQ=255)     gACCTTGTTGGTGCGCGTGCCGTCCAGTTCACTGCCGTAGATCACCTTGAAGTGATGATCGAACGCAAAGTGCCGAGCGATTTCGCGGGCGAACTCCCATGGTTTGGAGGTGGCAATGTACAGCGTGCGGCCCTGCCCGTTCAGGGCCt                                                                                                                                          >  6:199066/1‑149 (MQ=255)     gACCTTGTTGGTGCGCGTGCCGTCCAGTTCACTGCCGTAGATCACCTTGAAGTGATGATCGAACGCAAAGTGCCGAGCGATTTCGCGGGCGAACTCCCATGGTTTGGAGGTGGCAATGTACAGCGTGCGGCCCTGCCCGTTCAGGGCCt                                                                                                                                          >  7:132840/1‑149 (MQ=255)       ccTTGTTGGTGCGCGTGCCGTCCAGTTCACTGCCGTAGATCACCTTGAAGTGATGATCGAACGCAAAGTGCCGAGCGATTTCGCGGGCGAACTCCCATGGTTTGGAGGTGGCAATGTACAGCGTGCGGCCCTGCCCGTTCAGGGCCTc                                                                                                                                         >  3:30768/1‑148 (MQ=255)        cTTGTTGGTGCGCGTGCCGTTCAGTTCACTGCCGTAGATCACCTTGAAGTGATGATCGAACGCAAAGTGCCGAGCGATTTCGCGGGCGAACTCCCATGGTTTGGAGGTGGCAATGTACAGCGTGCGGCCCTGCCCGTTCAGGGCCTCaa                                                                                                                                       <  4:20053/149‑1 (MQ=255)        cTTGTTGGTGCGCGTGCCGTCCAGTTCACTGCCGTAGATCACCTTGAAGTGATGATCGAACTCAAAGTGCCGAGCGATTTCGCGGGCGAACTCCCATGGTTTGGAGGTGGCAATGTACAGCGTGCGGCCCTGCCCGTTCAGGGCCTCaa                                                                                                                                       <  4:30768/149‑1 (MQ=255)        cTTGTTGGTGCGCGTGCCGTCCAGTTCACTGCCGTAGATCACCTTGAAGTGATGATCGAACGCAAAGTGCCGAGCGATTTCGCGGGCGAACTCCCATGGTTTGGAGGTGGCAATGTACAGCGTGCGGCCCTGCCCGTTCAGGGCCTCaa                                                                                                                                       <  5:43381/149‑1 (MQ=255)           gttgGTGCGCGTGCCGTCCAGTTCACTGCCGTAGATCACCTTGAAGTGATGATCGAACGCAAAGTGCCGAGCGATTTCGCGGGCGAACTCCCATGGTTTGGAGGTGGCAATGTACAGCGTGCGGCCCTGCCCGTTCAGGGCCTCAA‑Gc                                                                                                                                    <  1:147845/148‑1 (MQ=255)             tgGTGCGCGTGCCGTCCAGTTCACTGCCGTAGATCACCTTGAAGTGATGATCGAACGCAAAGTGCCGAGCGATTTCGCGGGCGAACTCCCATGGGTTGGAGGTGGCAATGTACAGCGTGCGGCCCTGCCCGTTCAGGGCCTCAA‑GCaa                                                                                                                                  >  4:244142/1‑148 (MQ=255)                   gcgTGCCGTCCAGTTCACTGCCGTAGATCACCTTGAAGTGATGATCGAACGCAAAGTGCCGAGCGATTTCGCGGGCGAACTCCCATGGTTTGGAGGTGGCAATGTACAGCGTGCGGCCCTGCCCGTTCAGGGCCTCAA‑GCAACTCCGGc                                                                                                                           <  5:199066/149‑1 (MQ=255)                                ttCACTGCCGTAGATCACCTTGAAGTGATGATCGAACGCAAAGTGCCGAGCGATTTCGCGGGCGAACTCCCATGGTTTGGAGGTGGCAATGTACAGCGTGCGGCCCTGCCCGTTCAGGGCCTCAA‑GCAACTCCGGCAC‑ACCtt                                                                                                                   >  7:39440/1‑143 (MQ=255)                                ttCACTGCCGTAGATCACCTTGAAGTGATGATCGAACGCAAAGTGCCGAGCGATTTCGCGGGCGAACTCCCATGGTTTGGAGGTGGCAATGTACAGCGTGCGGCCCTGCCCGTTCAGGGCCTCAA‑GCAACTCCGGCAC‑ACCtt                                                                                                                   <  8:39440/143‑1 (MQ=255)                                       ccGTAGATCACCTTGAAGTGATGATCGAACGCAAAGTGCCGAGCGATTTCGCGGGCGAACTCCCATGGTTTGGGGGTGGCAATGTACAGCGTGCGGCCCTGCCCGTTCAGGGCCTCAATGCAACTCCGGCAC‑ACCTTCGAACACGAgg                                                                                                        <  1:26581/148‑1 (MQ=255)                                         gTAGATCACCTTGAAGTGATGATCGAACGCAAAGTGCCGAGCGATTTCGCGGGCGAACTCCCATGGTTTGGAGGTGGCAATGTACAGCGTGCGGCCCTGCCCGTTCAGGGCCTCAA‑GCAACTCCGGCAC‑ACCTTCGAACACGAGGtttt                                                                                                    >  1:142950/1‑149 (MQ=255)                                          tAGATCACCTTGAAGTGATGATCGAACGCAAAGTGCCGAGCGATTTCGCGGGCGAACTCCCATGGTTTGGAGGTGGCAATGTACAGCGTGCGGCCCTGCCCGTTCAGGGCCTCAA‑GCAACTCCGGCAC‑ACCTTCGAACACGAGGTTTTc                                                                                                   <  2:142950/149‑1 (MQ=255)                                               cACCTTGAAGTGATGATCGAACGCAAAGTGCCGAGCGATTTCGCGGGCGAACTCCCATGGTTTGGAGGTGGCAATGTACAGCGTGCGGCCCTGCCCGTTCAGGGCCTCAA‑GCAACTCCGGcac                                                                                                                         <  2:195656/123‑1 (MQ=255)                                               cACCTTGAAGTGATGATCGAACGCAAAGTGCCGAGCGATTTCGCGGGCGAACTCCCATGGTTTGGAGGTGGCAATGTACAGCGTGCGGCCCTGCCCGTTCAGGGCCTCAA‑GCAACTCCGGcac                                                                                                                         >  1:195656/1‑123 (MQ=255)                                                aCCTTGAAGTGATGATCGAACGCAAAGTGCCGAGCGATTTCGCGGGCGAACTCCCATGGTTTGGAGGTGGCAATGTACAGCGTGCGGCCCTGCCCGTTCAGGGCCTCAA‑GCAACTCCGGCAC‑ACCTTCGAACACGAGGTTTTCGTaaa                                                                                              <  7:170232/148‑1 (MQ=255)                                                  cTTGAAGTGATGATCGAACGCAAAGTGCCGAGCGATTTCGCGGGCGAACTCCCATGGTTTGGAGGTGGCAATGTACAGCGTGCGGCCCTGCCCGTTCAGGGCCTCAA‑GCAACTCCGGCAC‑ACCTTCGAACACGAg                                                                                                         >  4:248918/1‑135 (MQ=255)                                                  cTTGAAGTGATGATCGAACGCAAAGTGCCGAGCGATTTCGCGGGCGAACTCCCATGGTTTGGAGGTGGCAATGTACAGCGTGCGGCCCTGCCCGTTCAGGGCCTCAA‑GCAACTCCGGCAC‑ACCTTCGAACACGAg                                                                                                         <  3:248918/135‑1 (MQ=255)                                                             gatCGAACGCAAAGTGCCGAGCGATTTCGCGGGCGAACTCCCATGGTTTGGAGGTGGCAATGTACAGCGTGCGGCCCTGCCCGTTCAGGGCCTCAA‑GCAACTCCGGCAC‑ACCTTCGAACACGAGGTTTTCGTAAAGACCGGTCAcgcg                                                                                 >  3:145825/1‑148 (MQ=255)                                                              atCGAACGCAAAGTGCCGAGCGATTTCGCGGGCGAACTCCCATGGTTTGGAGGTGGCAATGTACAGCGTGCGGCCCTGCCCGTTCAGGGCCTCAA‑GCAACTCCGGCAC‑ACCTTCGAACACGAg                                                                                                         <  1:229207/123‑1 (MQ=255)                                                              atCGAACGCAAAGTGCCGAGCGATTTCGCGGGCGAACTCCCATGGTTTGGAGGTGGCAATGTACAGCGTGCGGCCCTGCCCGTTCAGGGCCTCAA‑GCAACTCACGCAC‑CCCATCGAACACGAg                                                                                                         >  2:229207/1‑123 (MQ=255)                                                               tCGAACGCAAAGTGCCGAGCGATTTCGCGGGCGAACTCCCATGGTTTGGAGGTGGCAATGTACAGCGTGCGGCCCTGCCCGTTCAGGGCCTCAA‑GCAACTCCGGCAC‑ACCTTCGAACACGAGGTTTTCGTAAAGACCGGTCACGCGGaa                                                                              <  3:244142/149‑1 (MQ=255)                                                                 gAACGCAAAGTGCCGAGCGATTTCGCGGGCGAACTCCCATGGTTTGGAGGTGGCAATGTACAGCGTGCGGCCCTGCCCGTTCAGGGCCTCAA‑GCAACTCCGGCAC‑ACCTTCGAACACGAGGTTTTCGTAAAGACCGGTCACGCGGAAg                                                                             <  4:145825/148‑1 (MQ=255)                                                                              cGAGCGATTTCGCGGGCGAACTCCCATGGTTTGGAGGTGGCAATGTACCGCGTGCGGCCCTGCCCGTTCAGGGCCTCAA‑GCAACTCCGGCAC‑ACCTTCGAACACGAGGTTTTCGTAAAGACCGGTCACGCGGAAGCGCTCGCGGTAg                                                                 >  8:208808/1‑147 (MQ=255)                                                                               gAGCGATTTCGCGGGCGAACTCCCATGGTTTGGAGGTGGCAATGTACCGCGTGCGGCCCTGCCCGTTCAGGGCCTCAA‑GCAACTCCGGCAC‑ACCTTCGAACACGAGGTTTTCGTAAAGACCGGTCACGCGGAAGCGCTCGCGGTAGAAg                                                              <  7:208808/149‑1 (MQ=255)                                                                               gAGCGATTTCGCGGGCGAACTCCCATGGTTTGGAGGTGGCAATGTACAGCGTGCGGCCCTGCCCGTTCAGGGCCTCAA‑GCAACTCCGGCAC‑ACCTTCGAACACGAGGtttt                                                                                                    >  6:25104/1‑111 (MQ=255)                                                                               gAGCGATTTCGCGGGCGAACTCCCATGGTTTGGAGGTGGCAATGTACAGCGTGCGGCCCTGCCCGTTCAGGGCCTCAA‑GCAACTCCGGCAC‑ACCTTCGAACACGAGGtttt                                                                                                    <  5:25104/111‑1 (MQ=255)                                                                                        cgcgGGCGAACTCCCATGGTTTGGAGGTGGCAATGTACAGCGTGCGGCCCTGCCCGTTCAGGGCCTCAA‑GCAACTCCGGCAC‑ACCTTCGAACACGAGGTTTTCGTAAAGACCGGTCACGCGGAAGCGCTCGCGGTAGAAGTTCACCGCa                                                     >  7:1547/1‑149 (MQ=255)                                                                                               gAACTCCCATGGTTTGGAGGTGGCAATGTACAGCGTGCGGCCCTGCCCGTTCAGGGCCTCAA‑GCAACTCCGGCAC‑ACCTTCGAACACGAGGTTTTCGTAAAGACCGGTCACGCGGAAGCGCTCGCGGt                                                                   <  2:170327/128‑1 (MQ=255)                                                                                               gAACTCCCATGGTTTGGAGGTGGCAATGTACAGCGTGCGGCCCTGCCCGTTCAGGGCCTCAA‑GCAACTCCGGCAC‑ACCTTCGAACACGAGGTTTTCGTAAAGACCGGTCACGCGGAAGCGCTCGCGGt                                                                   >  1:170327/1‑128 (MQ=255)                                                                                               gAACTCCCATGGTTTGGAGGTGGCAATGTACAGCGTGCGGCCCTGCCCGTTCAGGGCCTCAA‑GCAACTCCGGCAC‑ACCTTCGAACACGAGGTTTTCGTAAAGACCGGTCACGCGGAAGCGCTCGCGGTAGAAGTTCACCGCATCCCAgg                                              >  7:275481/1‑149 (MQ=255)                                                                                               gAACTCCCATGGTTTGGAGGTGGCAATGTACAGCGTGCGGCCCTGCCCGTTCAGGGCCTCAA‑GCAACTCCGGCAC‑ACCTTCGAACACGAGGTTTTCGTAAAGACCGGTCACGCGGAAGCGCTCGCGGTAGAAGTTCACCGCATCCCAgg                                              >  8:98325/1‑149 (MQ=255)                                                                                                     ccATGGTTTGGAGGTGGCAATGTACAGCGTGCGGCCCTGCCCGTTCAGGGCCTCAA‑GCAACTCCGGCAC‑ACCTTCGAACACGAGGTTTTCGTaaa                                                                                              <  7:170082/95‑1 (MQ=255)                                                                                                     ccATGGTTTGGAGGTGGCAATGTACAGCGTGCGGCCCTGCCCGTTCAGGGCCTCAA‑GCAACTCCGGCAC‑ACCTTCGAACACGAGGTTTTCGTaaa                                                                                              >  8:170082/1‑95 (MQ=255)                                                                                                     ccATGGTTTGGAGGTGGCAATGTACAGCGTGCGGCCCTGCCCGTTCAGGGCCTCAA‑GCAACTCCGGCAC‑ACCTTCGAACACGAGGTTTTCGTAAAGACCGGTCACGCGGAAGCGCTCGCGGTAGaa                                                               <  2:94543/126‑1 (MQ=255)                                                                                                     ccATGGTTTGGAGGTGGCAATGTACAGCGTGCGGCCCTGCCCGTTCAGGGCCTCAA‑GCAACTCCGGCAC‑ACCTTCGAACACGAGGTTTTCGTAAAGACCGGTCACGCGGAAGCGCTCGCGGTAGAAGTTCAcc                                                        <  5:139893/133‑1 (MQ=255)                                                                                                     ccATGGTTTGGAGGTGGCAATGTACAGCGTGCGGCCCTGCCCGTTCAGGGCCTCAA‑GCAACTCCGGCAC‑ACCTTCGAACACGAGGTTTTCGTAAAGACCGGTCACGCGGAAGCGCTCGCGGTAGAAGTTCAcc                                                        >  6:139893/1‑133 (MQ=255)                                                                                                     ccATGGTTTGGAGGTGGCAATGTACAGCGTGCGGCCCTGCCCGTTCAGGGCCTCAA‑GCAACTCCGGCAC‑ACCTTCGAACACGAGGTTTTCGTAAAGACCGGTCACGCGGAAGCGCTCGCGGTAGAAGTTCACCGCATCCCAGGCCTTgg                                        >  2:290034/1‑149 (MQ=255)                                                                                                      cATGGTTTGGAGGTGGCAATGTACAGCGTGCGGCCCTGCCCGTTCAGGGCCTCAA‑GCAACTCCGGCAC‑ACCTTCGAACACGAgg                                                                                                        <  1:349823/84‑1 (MQ=255)                                                                                                      cATGGTTTGGAGGTGGCAATGTACAGCGTGCGGCCCTGCCCGTTCAGGGCCTCAA‑GCAACTCCGGCAC‑ACCTTCGAACACGAgg                                                                                                        >  2:349823/1‑84 (MQ=255)                                                                                                      cATGGTTTGGAGGTGGCAATGTACAGCGTGCGGCCCTACCCGTTCAGGGCCTCAA‑GCAACTCCGGCAC‑ACCTTCGAACACGAGGTTTTCGTAAAGACCGGTCACGCGGAAGCGCTCGCGGTAGAAGTTCACCGCATCCCAGGCCTTGGc                                       <  7:126128/149‑1 (MQ=255)                                                                                                           tttGGAGGTGGCAATGTACAGCGTGCGGCCCTGCCCGTTCAGGGCCTCAA‑GCAACTCCGGCAC‑ACCTTCGAACACGAGGTTTTCGTAAAGACCGGTCACGCGGAAGCGCTCGCGGTAGAAGTTCACCGCATCCCAGGCCTTGGCTtcgt                                  <  8:132840/149‑1 (MQ=255)                                                                                                               gAGGTGGCAATGTACAGCGTGCGGCCCTGCCCGTTCAGGGCCTCAA‑GCAACTCCGGCAC‑ACCTTCGAACACGAGGTTTTCGTAAAGACCGGTCACGCGGAAGCGCTCGCGGTAGAAGTTCACCGCATCCCAGGCCTTGGCTtcgtcg                                >  6:216776/1‑147 (MQ=255)                                                                                                               gAGGTGGCAATGTACAGCGTGCGGCCCTGCCCGTTCAGGGCCTCAA‑GCAACTCCGGCAC‑ACCTTCGAACACGAGGTTTTCGTAAAGACCGGTCACGCGGAAGCGCTCGCGGTAGAAGTTCACCGCATCCCAGGCCTTGGCTTCGTCGaa                              <  5:249292/149‑1 (MQ=255)                                                                                                                aGGTGGCAATGTACAGCGTGCGGCCCTGCCCGTTCAGGGCCTCAA‑GCAACTCCGGCAC‑ACCTTCGAACACGAGGTTTTCGTAAAGACCGGTCACGCGGAAGCGCTCGCGGTAGAAGTTCACCGCATCCCAGGCCTTGGCTTCGTCGAAg                             >  4:33119/1‑149 (MQ=255)                                                                                                                     gCAATGTACAGCGTGCGGCCCTGCCCGTTCAGGGCCTCAA‑GCAACTCCGGCAC‑ACCTTCGAACACGAGGTTTTCGTAAAGACCGGTCACGCGGAAGCGCTCGCGGTAGAAGTTCACCGCATCCCAGGCCTTGGCTTCGTCGAAGCTg                          >  1:281728/1‑147 (MQ=255)                                                                                                                             cAGCGTGCGGCCCTGCCCGTTCAGGGCCTCAA‑GCAACTCCGGCAC‑ACCTTCGAACACGAGGTTTTCGTAAAGACCGGTCACGCGGAAGCGCTCGCGGTAGAAGTTCACCGCATCCCAGGCCTTGGCTTCGTCGAAGCTGTAGAACTGc                 <  8:1547/148‑1 (MQ=255)                                                                                                                              aGCGTGCGGCCCTGCCCGTTCAGGGCCTCAA‑GCAACTCCGGCAC‑ACCTTCGAACACGAGGTTTTCGTAAAGACCGGTCACGCGGAAGCGCTCGCGGTAGAAGTTCACCGCATCCCAGGCCTTgg                                        <  2:321577/124‑1 (MQ=255)                                                                                                                              aGCGTGCGGCCCTGCCCGTTCAGGGCCTCAA‑GCAACTCCGGCAC‑ACCTTCGAACACGAGGTTTTCGTAAAGACCGGTCACGCGGAAGCGCTCGCGGTAGAAGTTCACCGCATCCCAGGCCTTgg                                        >  1:321577/1‑124 (MQ=255)                                                                                                                              aGCGTGCGGCCCTGCCCGTTCAGGGCCTCAA‑GCAACTCCGGCAC‑ACCTTCGAACACGAGGTTTTCGTAAAGACCGGTCACGCGGAAGCGCTCGCGGTAGAAGTTCACCGCATCCCAGGCCTTGGCTtcgt                                  >  6:312614/1‑130 (MQ=255)                                                                                                                              aGCGTGCGGCCCTGCCCGTTCAGGGCCTCAA‑GCAACTCCGGCAC‑ACCTTCGAACACGAGGTTTTCGTAAAGACCGGTCACGCGGAAGCGCTCGCGGTAGAAGTTCACCGCATCCCAGGCCTTGGCTtcgt                                  <  5:312614/130‑1 (MQ=255)                                                                                                                              aGCGTGCGGCCCTGCCCGTTCAGGGCCTCAA‑GCAACTCCGGCAC‑ACCTTCGAACACGAGGTTTTCGTAAAGACCGGTCACGCGGAAGCGCTCGCGGTAGAAGTTCACCGCATCCCAGGCCTTGGCTTCGTCGAAGCTGTAGaa                     <  7:140563/143‑1 (MQ=255)                                                                                                                              aGCGTGCGGCCCTGCCCGTTCAGGGCCTCAA‑GCAACTCCGGCAC‑ACCTTCGAACACGAGGTTTTCGTAAAGACCGGTCACGCGGAAGCGCTCGCGGTAGAAGTTCACCGCATCCCAGGCCTTGGCTTCGTCGAAGCTGTAGaa                     >  8:140563/1‑143 (MQ=255)                                                                                                                                 ttgaggCCCTGAACGGGCAGGGCCGCAA‑GCTACTCCGGCAC‑ACCTTCGAACACGAGGTTTTCGTAAAGACCGGTCACGCGGAAGCGCTCGCGGTAGAAGTTCACCGCATCCCAGGCCTTGGCTTCGTCGAAGCTGTAGAACTGCa                >  1:29011/5‑145 (MQ=255)                                                                                                                                 ttgaggCCCTGAACGGGCAGGGCCGCAA‑GCAACTCCGGCAC‑ACCTTCGAACACGAGGTTTTCGTAAAGACCGGTCACGCGGAAGCGCTCGCGGTAGAAGTTCACCGCATCCCAGGCCTTGGCTTCGTCGAAGCTGTAGAACTGCa                <  2:29011/141‑1 (MQ=255)                                                                                                                                 gTGCGGCCCTGCCCGTTCAGGGCCTCAA‑GCAACTCCGGCAC‑ACCTTCGAACACGAGGTTTTCGTAAAGACCGGTCACGCGGAAGCGCTcg                                                                       <  3:287298/90‑1 (MQ=255)                                                                                                                                 gTGCGGCCCTGCCCGTTCAGGGCCTCAA‑GCAACTCCGGCAC‑ACCTTCGAACACGAGGTTTTCGTAAAGACCGGTCACGCGGAAGCGCTcg                                                                       >  4:287298/1‑90 (MQ=255)                                                                                                                                  tGCGGCCCTGCCCGTTCAGGGCCTCAA‑GCAACTCCGGCAC‑ACCTTCGAACACGAGGTTTTCGTAAAGACCGGTCACGCGGAAGCGCTCGCGGTAGAAGTTCACCGCATCCCAGGCCTTGGCTTCGTCGAAGCTGTAGAACTGCATGaaa           <  8:275481/149‑1 (MQ=255)                                                                                                                                  tGCGGCCCTGCCCGTTCAGGGCCTCAA‑GCAACTCCGGCAC‑ACCTTCGAACACGAGGTTTTCGTAAAGACCGGTCACGCGGAAGCGCTCGCGGTAGAAGTTCACCGCATCCCAGGCCTTGGCTTCGTCGAAGCTGTAGAACTGCATGa             >  6:275985/1‑147 (MQ=255)                                                                                                                                     ggCCCTGACCGGGCAGGGCCTCAA‑GCAACTCCGGCAC‑TCCTTCGAACACGAGGTTTTCGTAAAGACCGGTCACGCGGAAGCGCTCGCGGTAGAAGTTCACCGCATCCCAGGCCTTGGCTTCGTCGAAGCTGTAGAACTGCATGaaa           <  1:290200/146‑1 (MQ=255)                                                                                                                                     ggCCCTGAACGGGCAGGGCCTCAA‑GCAACTCCGGCAC‑ACCTTCGAACACGAGGTTTTCGTAAAGACCGGTCACGCGGAAGCGCTCGCGGTAGAAGTTCACCGCATCCCAGGCCTTGGCTTCGTCGAAGCTGTAGAACTGCATGaaa           >  2:290200/1‑146 (MQ=255)                                                                                                                                      gCCCTGCCCGTTCAGGGCCTCAA‑GCAACTCCGGCAC‑ACCTTCGAACACGAGGTTTTCGTAAAGACCGGTCACGCGGAAGCGCTCGCGGTAGAAGTTCACCGCATCCCAGGCCTTGGCTTCGTCGAAGCTGTAGAACTGCATGAAAGCCt       >  8:196796/1‑149 (MQ=255)                                                                                                                                       cccTGCCCGTTCAGGGCCTCAA‑GCAACTCCGGCAC‑ACCTTCGAACACGAGGTTTTCGTAAAGACCGGTCACGCGGAAGCGCTCGCGGTAGAAGTTCACCGCATCCCAGGCCTTGGCTTCGTCGAAGCTGTa                        <  8:161933/131‑1 (MQ=255)                                                                                                                                       cccTGCCCGTTCAGGGCCTCAA‑GCAACTCCGGCAC‑ACCTTCGAACACGAGGTTTTCGTAAAGACCGGTCACGCGGAAGCGCTCGCGGTAGAAGTTCACCGCATCCCAGGCCTTGGCTTCGTCGAAGCTGTa                        >  7:161933/1‑131 (MQ=255)                                                                                                                                       cccTGCCCGTTCAGGGCCTCAA‑GCAACTCCGGCAC‑ACCTTCGAACACGAGGTTTTCGTAAAGACCGGTCACGCGGAAGCGCTCGCGGTAGAAGTTCACCGCATCCCAGGCCTTGGCTTCGTCGAAGCTGTAGAACTGCATGaaa           >  8:138228/1‑144 (MQ=255)                                                                                                                                       cccTGCCCGTTCAGGGCCTCAA‑GCAACTCCGGCAC‑ACCTTCGAACACGAGGTTTTCGTAAAGACCGGTCACGCGGAAGCGCTCGCGGTAGAAGTTCACCGCATCCCAGGCCTTGGCTTCGTCGAAGCTGTAGAACTGCATGaaa           <  7:138228/144‑1 (MQ=255)                                                                                                                                         cTGCCCGTTCAGGGCCTCAA‑GCAACTCCGGCACGACCTTCGAACACGAGGTTTTCGTAAAGACCGGTCACGCGGAAGCGCTCGCGGTAGAAGTTCACCGCATCCCAGGCCTTGGCTTCGTCGAAGCTGTAGAACTGCATGAAAGCCTg      >  1:47669/1‑148 (MQ=255)                                                                                                                                            cccGTTCAGGGCCTCAA‑GCAACTCCGGCAC‑ACCTTCGAACACGAGGTTTTCGTAAAGACCGGTCACGCGGAAGCGCTCGCGGTAGAAGTTCACCGCATCCCAGGCCTTGGCTTCGTCGAAGCTGTAGAACTGCATGAAAGCCTgcag   >  5:137406/1‑147 (MQ=255)                                                                                                                                              cGTTCAGGGCCTCAA‑GCAACTCCGGCAC‑ACCTTCGAACACGAGGTTTTCGTAAAGACCGGTCACGCGGAAGCGCTCGCGGTAGAAGTTCACCGCATCCCAGGCCTTGGCTTCGTCGAAGCTGTAGAACTGCATGAAAGCCTgcagc  <  4:5880/146‑1 (MQ=255)                                                                                                                                              cGTTCAGGGCCTCAA‑GCAACTCCGGCAC‑ACCTTCGAACACGAGGTTTTCGTAAAGACCGGTCACGCGGAAGCGCTCGCGGTAGAAGTTCACCGCATCCCAGGCCTTGGCTTCGTCGAAGCTGTAGAACTGCATGAAAGCCTgcagc  >  3:5880/1‑146 (MQ=255)                                                                                                                                                 |                                                                                                                                                 GCTCGACCTTGTTGGTGCGCGTGCCGTCCAGTTCACTGCCGTAGATCACCTTGAAGTGATGATCGAACGCAAAGTGCCGAGCGATTTCGCGGGCGAACTCCCATGGTTTGGAGGTGGCAATGTACAGCGTGCGGCCCTGCCCGTTCAGGGCCTCAA‑GCAACTCCGGCAC‑ACCTTCGAACACGAGGTTTTCGTAAAGACCGGTCACGCGGAAGCGCTCGCGGTAGAAGTTCACCGCATCCCAGGCCTTGGCTTCGTCGAAGCTGTAGAACTGCATGAAAGCCTGCAGC  >  NC_002947/99973‑100259

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑