| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 5,988,809 | G→A | 17.5% | intergenic (+103/‑443) | kefB‑III → / → PP_mr64 | glutathione‑regulated potassium/H+ antiporter/C4 ncRNA |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 5,988,809 | 0 | G | A | 17.5% | 126.5 / 16.7 | 57 | intergenic (+103/‑443) | kefB‑III/PP_mr64 | glutathione‑regulated potassium/H+ antiporter/C4 ncRNA |
| Reads supporting (aligned to +/- strand): ref base G (19/28); new base A (6/4); total (25/32) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.08e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.73e-01 | |||||||||||
GTGGAGCTGGAGCGGTTGTTTGATTCGGATGCGGACTGAGGCCTTTTGGCGCGTGCCTTGAGGGTTGTAGTGCCTGTGAGATTGGGCGCCGCCCGCGCGGCGCTTCGCGGGTAAACCCGCTCCTACGTTTGTTTCGG‑GCCAGTTATTCCTGTGGGACGCGCACGCGAGCCCCCTGGCGCACGCCTCGATATCGCGTCGTACAAACAAGGCGGTCGCGCGCGCCTGGCACAGGAGGGATTGGCCAGAAACAAACGTAGGAGCGGGTTTACCCGCGAAGCGCCGCGCGGGCG > NC_002947/5988668‑5988957 | gTGGAGCTGGAGCGGTTGTTTGATTCGGATGCGGACTGAGGCCTTTTGGCGCGTGCCTTGAGGGTTGTAGTGCCTGTGAGATTGGGCGCCGCCCGCGCGGCGCTTCGCGGGTAAACCCGCTCCTACGTTTGTTTCGG‑GCCAGTTATTcc > 3:119303/1‑149 (MQ=255)gTGGAGCTGGAGCGGTTGTTTGATTCGGATGCGGACTGAGGCCTTTTGGCGCGTGCCTTGAGGGTTGTAGTGCCTGTGAGATTGGGCGCCGCCCGCGCGGCGCTTCGCGGGTAAACCCGCTCCTACGTTTGTTTCGG‑GCCAGTTATTcc < 5:335545/149‑1 (MQ=255) gcgcctgggaggtgttcgccgggggattttcagcgcctgggaggtgttcgccgggagagtttcagcgcctgggagatcgagcgccgcccgcgCGGCGCTTCGCGGGTAAACCCGCTCCTACGTTTGTTTCGG‑GCCAATTATTCCTGCgg > 5:126492‑M2/93‑149 (MQ=255) ggtggtgtttggtgggtgattttgtgcgcctgggaggtgttcgccgggagagtttcagcgcctgggagatcgagcgccgcccgcgCGGCGCTTCGCGGGTAAACCCGCTCCTACGTTTGTTTCGG‑GCCAATTATTCCTGCgggattt < 1:292170‑M2/62‑4 (MQ=255) cGGTTGTTTGATTCGGATGCGGACTGAGGCCTTTTGGCGCGTGCCTTGAGGGTTGTAGTGCCTGTGAGATTGGGCGCCGCCCGCGCGGCGCTTCGCGGGTAAACCCGCTCCTACGTTTGTTTCGG‑GCCAGTTATTCCTGTGGGACgcgc < 5:116386/149‑1 (MQ=255) tttGATTCGGATGCGGACTGAGGCCTTTTGGCGCGTGCCTTGAGGGTTGTAGTGCCTGTGAGATTGGGCGCCGCCCGCGCGGCGCTTCGCGGGTAAACCCGCTCCTACGTTTGTTTCGG‑GCCAGTTATTCCTGTGGGACGCGCACGCGa < 4:119303/149‑1 (MQ=255) ggATGCGGACTGAGGCCTTTTGGCGCGTGCCTTGAGGGTTGTAGTGCCTGTGAGATTGGGCGCCGCCCGCGCGGCGCTTCGCGGGTAAACCCGCTCCTACGTTTGTTTCGG‑GCCAGTTATTCCTGTGGGACGCGCACGCGAGCCCCCTg > 2:241142/1‑149 (MQ=255) ggATGCGGACTGAGGCCTTTTGGCGCGTGCCTTGAGGGTTGTAGTGCCTGTGAGATTGGGCGCCGCCCGCGCGGCGCTTCGCGGGTAAACCCGCTCCTACGTTTGTTTCGG‑GCCAGTTATTCCTGTGGGACGCGCACGCGAGCCCCCTg > 6:234433/1‑149 (MQ=255) tcagcgcctgggaggtgttcgccgggagagtttcagcgcctgggagatcgagcgccgcccgcgCGGCGCTTCGCGGGTAAACCCGCTCCTACGTTTGTTTCGG‑GCCAATTATTCCTGCGGGATTTGCGCGCGACCGCCTTGGggcatgt > 1:215381‑M2/64‑146 (MQ=255) gCCTTTTGGCGCGTGCCTTGAGGGTTGTAGTGCCTGTGAGATTGGGCGCCGCCCGCGCGGCGCTTCGCGGGTAAACCCGCTCCTACGTTTGTTTCGG‑GCCAGTTATTCCt < 2:312155/110‑1 (MQ=255) gCCTTTTGGCGCGTGCCTTGAGGGTTGTAGTGCCTGTGAGATTGGGCGCCGCCCGCGCGGCGCTTCGCGGGTAAACCCGCTCCTACGTTTGTTTCGG‑GCCAGTTATTCCt > 1:312155/1‑110 (MQ=255) gCCTTTTGGCGCGTGCCTTGAGGGTTGTAGTGCCTGTGAGATTGGGCGCCGCCCGCGCGGCGCTTCGCGGGTAAACCCGCTCCTACGTTTGTTTCGG‑GCCAGTTATTCCTGTGGGACGCGCACGCGAGCCCCCTGGCGCACGCCTCGa > 3:237478/1‑148 (MQ=255) cctgggaggtgttcgccgggagagtttcagcgcctgggagatcgagcgccgcccgcgCGGCGCTTCGCGGGTAAACCCGCTCCTACGTTTGTTTCGG‑GCCAATTATTCCTGCGGGATTTGCGCGCGACCGCCTTGGGGCATGTCTCGat < 8:142245‑M2/92‑1 (MQ=255) cctgggaggtgttcgccgggagagtttcagcgcctgggagatcgagcgccgcccgcgCGGCGCTTCGCGGGTAAACCCGCTCCTACGTTTGTTTCGG‑GCCAATTATTCCTGCGGGATTTGCGCGCGACCGCCTTGGGGCATGTCTCGa > 7:142245‑M2/58‑148 (MQ=255) ggaggtgttcgccgggagagtttcagcgcctgggagatcgagcgccgcccgcgCGGCGCTTCGCGGGTAAACCCGCTCCTACGTTTGTTTCGG‑GCCAATTATTCCTGCGGGATTTGCGCGCGACCGCCTTGGGGCATGTCTCGATATc < 5:132510‑M2/95‑1 (MQ=255) ggTTGTAGTGCCTGTGAGATTGGGCGCCGCCCGCGCGGCGCTTCGCGGGTAAACCCGCTCCTACGTTTGTTTCGG‑GCCAGTTATTCCTGTGGGACGCGCACGCGAGCGCCCTGGCGCACGCCTCGATATCGCGTCGTACAAACAAGGc < 4:237478/148‑1 (MQ=255) ggTTGTAGTGCCTGTGAGATTGGGCGCCGCCCGCGCGGCGCTTCGCGGGTAAACCCGCTCCTACGTTTGTTTCGG‑GCCAGTTATTCCTGTGGGACGCGCACGCGAGCCCCCTGGCGCACGCCTCGATATCGCGTCGTACAAACAAGGCg < 1:241142/149‑1 (MQ=255) ggTTGTAGTGCCTGTGAGATTGGGCGCCGCCCGCGCGGCGCTTCGCGGGTAAACCCGCTCCTACGTTTGTTTCGG‑GCCAGTTATTCCTGTGGGACGCGCACGCGAGCCCCCTGGCGCACGCCTCGATATCGCGTCGTACAAACAAGGCg < 5:234433/149‑1 (MQ=255) gTTGTAGTGCCTGTGAGATTGGGCGCCGCCCGCGCGGCGCTTCGCGGGTAAACCCGCTCCTACGTTTGTTTCGG‑GCCAGTTATTCCTGTGGGACGCGCACGCGAGCCCCCTGGCGCACGCCTCGATATCGCGTCGTACAAACAAGGCgg < 5:140407/149‑1 (MQ=255) gcgcctgggagatcgagcgccgcccgcgCGGCGCTTCGCGGGTAAACCCGCTCCTACGTTTGTTTCGG‑GCCAATTATTCCTGCGGGATTTGCGCGCGACCGCCTTGGGGCATGTCTCGATATCGCGTCGTac > 2:101808‑M2/29‑132 (MQ=255) gcgcctgggagatcgagcgccgcccgcgCGGCGCTTCGCGGGTAAACCCGCTCCTACGTTTGTTTCGG‑GCCAATTATTCCTGCGGGATTTGCGCGCGACCGCCTTGGGGCATGTCTCGATATCGCGTCGTac < 1:101808‑M2/104‑1 (MQ=255) tGCCTGTGAGATTGGGCGCCGCCCGCGCGGCGCTTCGCGGGTAAACCCGCTCCTACGTTTGTTTCGG‑GCCAGTTATTCCTGTGGGACGCGCACGCGAGCCCCCTGGCGCACGCCTCGATATCGCGTCGTACAAACAAGGCGGTcgcgc < 8:112595/148‑1 (MQ=255) ccTGTGAGATTGGGCGCCGCCCGCGCGGCGCTTCGCGGGTAAACCCGCTCCTACGTTTGTTTCGG‑GCCAGTTATTCCTGTGGGACGCGCACGCGAGCCCCCTGGCGCACGCCTCGa < 2:206574/116‑1 (MQ=255) ccTGTGAGATTGGGCGCCGCCCGCGCGGCGCTTCGCGGGTAAACCCGCTCCTACGTTTGTTTCGG‑GCCAGTTATTCCTGTGGGACGCGCACGCGAGCCCCCTGGCGCACGCCTCGa > 1:206574/1‑116 (MQ=255) ccTGTGAGATTGGGCGCCGCCCGCGCGGCGCTTCGCGGGTAAACCCGCTCCTACGTTTGTTTCGG‑GCCAGTTATTCCTGTGGGACGCGCACGCGAGCCCCCTGGCGCACGCCTCGATATCGCGTCGTACAAACAAGGCGGTcgcgcgcg > 5:252492/1‑149 (MQ=255) gtgAGATTGGGCGCCGCCCGCGCGGCGCTTCGCGGGTAAACCCGCTCCTACGTTTGTTTCGG‑GCCAGTTATTCCTGTGGGACGCGCACGCGAGc > 2:50030/1‑94 (MQ=255) gtgAGATTGGGCGCCGCCCGCGCGGCGCTTCGCGGGTAAACCCGCTCCTACGTTTGTTTCGG‑GCCAGTTATTCCTGTGGGACGCGCACGCGAGc < 1:50030/94‑1 (MQ=255) gtgAGATTGGGCGCCGCCCGCGCGGCGCTTCGCGGGTAAACCCGCTCCTACGTTTGTTTCGG‑GCCAGTTATTCCTGTGGGACGCGCACGCGAGCCCCCTGGCGCACGCCTCGATATCGCGTCGTACAAACAAGGCGGTCGCGCGCGCCt > 1:134161/1‑149 (MQ=255) tgAGATTGGGCGCCGCCCGCGCGGCGCTTCGCGGGTAAACCCGCTCCTACGTTTGTTTCGG‑GCCAGTTATTCCTGTGGGACGCGCACGCGAGCCCCCTGGCGCACGCCTCGATATCGCGTCGTACAAACAAGGCGGTCGCGCGCGCCTg > 4:75581/1‑149 (MQ=255) tgAGATTGGGCGCCGCCCGCGCGGCGCTTCGCGGGTAAACCCGCTCCTACGTTTGTATCGG‑GCCAGTTATTCCAGTGGGACGCGCACGCGAGCCCCCAGCCGCACGCCTCGATATCGCGTCGTACAAACACGGCGGTCGCTCGccccgg > 4:75582/1‑147 (MQ=25) gcgaagcgcCGCGCGGGCGGCGCTTCGCGGGTAAACCCGCTCCTACGTTTGTTTCGG‑GCCAATTATTCCTGCGGGATTTGCGCGCGACCGCCTTGGGGCATGTCTCGATATCGCGTCGTACAAACAAGGCGGTCGCGCGCGACTggt > 7:271839/6‑146 (MQ=14) gcgccgcccgcgCGGCGCTTCGCGGGTAAACCCGCTCCTACGTTTGTTTCGGCGTCCGTTATCTCGCCCCCTCGCGCACGCGAGCCCCCTGGCGCACGCCTCGATATCGCGTCGTACAAACAAGGCGGTCGCGCGCGCCTGGCACagga < 1:112403‑M2/137‑1 (MQ=255) gcgccgcccgcgCGGCGCTTCGCGGGTAAACCCGCTCCTACGTTTGTTTCGG‑GCCAGTTATTCCTGTGGGACGCGCACGCGAGCCCCCTGGCGCACGCCTCGATATCGCGTCGTACAAACAAGGCGGTCGCGCGCGCCTGGCACaggag < 4:192081‑M2/137‑1 (MQ=255) gccgcccgcgCGGCGCTTCGCGGGTAAACCCGCTCCTACGTTTGTTTCGG‑GCCAGTTATTCCTGTGGGACGCGCACGCGAGCCCCCTGGCGCACGCCTCGATATCGCGTCGTACAAACAAGGCGGTCGCGCGCGCCTGGCACAGGAggg < 1:198741‑M2/139‑1 (MQ=255) ccgcccgcgCGGCGCTTCGCGGGTAAACCCGCTCCTACGTTTGTTTCGG‑GCCAGTTATTCCTGTGGGACGCGCACGCGAGCCCCCTGGCGCACGCCTCGATATCGCGTCGTACAAACAAGGCg > 4:130977‑M2/10‑123 (MQ=255) ccgcccgcgCGGCGCTTCGCGGGTAAACCCGCTCCTACGTTTGTTTCGG‑GCCAGTTATTCCTGTGGGACGCGCACGCGAGCCCCCTGGCGCACGCCTCGATATCGCGTCGTACAAACAAGGCg < 3:130977‑M2/114‑1 (MQ=255) cgcccgcgCGGCGCTTCGCGGGTAAACCCGCTCCTACGTTTGTTTCGG‑GCCAGTTATTCCTGTGGGACGCGCACGCGAGCCCCCTGGCGCACGCCTCGATATcgcg < 7:2981‑M2/98‑1 (MQ=255) cgcccgcgCGGCGCTTCGCGGGTAAACCCGCTCCTACGTTTGTTTCGG‑GCCAGTTATTCCTGTGGGACGCGCACGCGAGCCCCCTGGCGCACGCCTCGATATcgcg > 8:2981‑M2/9‑106 (MQ=255) gcccgcgCGGCGCTTCGCGGGTTAAACCGCTACTAAGTTTGTTTCGG‑GCCAGTTTTTGCTGTGGGACGCGCACGCGAGCCCCCTGGCGCACGCCTCGATATGGCGGCGGTCCAACAAGGCGGTCGCGCGCGCCTGGCACAGGAGGGAtt > 6:8258‑M2/8‑149 (MQ=255) cccgcgGGGCGCTGCGCGGGTCCCCCCGCTCCTCCGGTGGGTTCGG‑GCCCGTTCTTCCTGTGGGACGCGCACGCGAGCCCCCTGGCGCACGCCTCGATATCGCGTCGTACAAACAAGGCGGTCGCGCGCGCCTGGCACAGGAGGGATTg < 2:134161‑M2/143‑1 (MQ=255) cccgcgCGGCGCTTCGCGGGTAACCCCGCTCCGACGTTTGTTTCGG‑GCCAGTTATTCCTGTGGGACGCGCTCGCGAGCCCCCTGGCGCACGCCTCGATATCGCGGCGTACTAACAAGGCGGTCGCGCGCGCCTGGCACAGGAGGGTTTg < 3:75582‑M2/143‑1 (MQ=255) cccgcgCGGCGCTTCGCGGGTAAACCCGCTCCTACGTTTGTTTCGG‑GCCAGTTATTCCTGTGGGACGCGCACGCGAGCCCCCTGGCGCACGCCTCGATATCGCGTCGTACAAACAAGGCGGTCGCGCGCGCCTGGCACAGGAGGGATTg > 5:268895‑M2/7‑149 (MQ=255) cccgcgCGGCGCTTCGCGGGTAAACCCGCTCCTACGTTTGTTTCGG‑GCCAGTTATTCCTGTGGGACGCGCACGCGAGCCCCCTGGCGCACGCCTCGATATCGCGTCGTACAAACAAGGCGGTCGCGCGCGCCTGGCACAGGAGGGATTg < 3:75581‑M2/143‑1 (MQ=255) cgcgCGGCGCTTCGCGGGTAAACCCGCTCCTACGTTTGTTTCGG‑GCCAGTTATTCCTGTGGGACGCGCACGCGAGCCCCCTGGCGCACGCCTCGATATCGCGTCGTACAAACAAGGCGGTCGCGCGCGCCTGGCACAGGAGGGATTGGc < 3:242363‑M2/145‑1 (MQ=255) gcgCGGCGCTTCGCGGGTAAACCCGCTCCTACGTTTGTTTCGG‑GCCAGTTATTCCTGTGGGACGCGCACGCGAGCCCCCTGGCGCACGCCTCGATATCGCGTCGTACAAACAAGGCGGTCGCGCGCGCCTGGCACAGGAGGGATTGGcc < 6:160473‑M2/146‑1 (MQ=255) gcgCGGCGCTTCGCGGGTAAACCCGCTCCTACGTTTGTTTCGG‑GCCAGTTATTCCTGTGGGACGCGCACGCGAGCCCCCTGGCGCACGCCTCGATATCGCGTCGTACAAACAAGGCGGTCGCGCGCGCCTGGCACAGGAGGGATTGGc > 5:160473‑M2/4‑148 (MQ=255) cgcTTCGCGGGTAAACCCGCTCCTACGTTTGTTTCGG‑GCCAGTTATTCCTGTGGGACGCGCACGCGAGCCCCCTGGCGCACGCCTCGATATCGCGTCGTACAAACAAGGCGGTCGCGCGCGCCTGGCACAGGAGGGATTGGCCAGaaac < 6:252492/149‑1 (MQ=255) cTTCGCGGGTAAACCCGCTCCTACGTTTGTTTCGG‑GCCAGTTATTCCTGTGGGACGCGCACGCGAGCCCCCTGGCGCACGCCTCGATATCGCGTCGTACAAACAAGGCGGTCGCGCGCGCCTGGCACAGGAGGGATTg > 4:323468/1‑138 (MQ=255) cTTCGCGGGTAAACCCGCTCCTACGTTTGTTTCGG‑GCCAGTTATTCCTGTGGGACGCGCACGCGAGCCCCCTGGCGCACGCCTCGATATCGCGTCGTACAAACAAGGCGGTCGCGCGCGCCTGGCACAGGAGGGATTg < 3:323468/138‑1 (MQ=255) cgcgGGTAAACCCGCTCCTACGTTTGTTTCGG‑GCCAGTTATTCCTGTGGGACGCGCACGCGAGCCCCCTGGCGCACGCCTCGATATCGCGTCGTACAAACAAGGCGGTCGCGCGCGCCTGGCACAGGAGGGATTGGCCAGaaacaaac < 6:268895/148‑1 (MQ=255) cgcgGGTAAACCCGCTCCTACGTTTGTTTCGG‑GCCAGTTATTCCTGTGGGACGCGCACGCGAGCCCCCTGGCGCACGCCTCGATATCGCGTCGTACAAACAAGGCGGTCGCGCGCGCCTGGCACAGGAGGGATTGGCCAGaaacaaac < 4:75549/148‑1 (MQ=255) ccGCTCCTACGTTTGTTTCGG‑GCCAGTTATTCCTGTGGGACGCGCACGCGAGCCCCCTGGCGCACGCCTCGATATCGCGTCGTACAAACAAGGCGGTCGCGCGCGCCTGGCACAGGAGGGATTGGCCAGAAACAAACGTAGGAGCGGGt > 7:66268/1‑149 (MQ=255) cTACGTTTGTTTCGG‑GCCAGTTATTCCTGTGGGACGCGCACGCGAGCCCCCTGGCGCACGCCTCGATATCGCGTCGTACAAACAAGGCGGTCGCGCGCGCCTGGCACAGGAGGGATTGGCCAGAAACAAACGTAGGAGCGGGTTTAccc < 4:157095‑M2/149‑4 (MQ=255) cTACGTTTGTTTCGG‑GCCAGTTATTCCTGTGGGACGCGCACGCGAGCCCCCTGGCGCACGCCTCGATATCGCGTCGTACAAACAAGGCGGTCGCGCGCGCCTGGCACAGGAGGGATTGGCCAGAAACAAACGTAGGAGCGGGTTTAccc > 5:8258‑M2/1‑146 (MQ=255) gtttgtttCGG‑GCCAGTTATTCCTGTGGGACGCGCACGCGAGCCCCCTGGCGCACGCCTCGATATCGCGTCGTACAAACAAGGCGGTCGCGCGCGCCTGGCACAGGAGGGATTGGCCAGAAACAAACGTAGGAGCGGGTTTAcccgcg < 4:67872‑M2/148‑7 (MQ=255) gCCAATTATTCCTGCGGGATTTGCGCGCGACCGCCTTGGGGCATGTCTCGATATCGCGTCGTACAAACAAGGCGGTCGCGCGCGACTGGTGCAGGAGGGACTGGCC‑GAAACAAACGTAGGAGCGGGTTTACCCGCGAAGCGCCGCgcgg > 5:82650/1‑149 (MQ=14) aGTTATTCCTGTGGGACGCGCACGCGAGCCCCCTGGCGCACGCCTCGATATCGCGTCGTACAAACAAGGCGGTCGCGCGCGCCTGGCACAGGAGGGATTGGCCAGAAACAAACGTAGGAGCGGGTTTAcccgcgaagcgccgcgcgggc < 6:192078‑M2/149‑22 (MQ=255) gTTATTCCTGGGGGACGCGCACGCGAGCCCCCTGGCGCACGCCTCGATATCGCGTCGTACAAACAAGGCGGTCGCGCGCGCCTGGCACAGGAGGGATTGGCCAGAAACAAACGTAGGAGCGGGTTTAcccgcgaagcgccgcgcgggcg < 2:196474‑M2/149‑23 (MQ=255) | GTGGAGCTGGAGCGGTTGTTTGATTCGGATGCGGACTGAGGCCTTTTGGCGCGTGCCTTGAGGGTTGTAGTGCCTGTGAGATTGGGCGCCGCCCGCGCGGCGCTTCGCGGGTAAACCCGCTCCTACGTTTGTTTCGG‑GCCAGTTATTCCTGTGGGACGCGCACGCGAGCCCCCTGGCGCACGCCTCGATATCGCGTCGTACAAACAAGGCGGTCGCGCGCGCCTGGCACAGGAGGGATTGGCCAGAAACAAACGTAGGAGCGGGTTTACCCGCGAAGCGCCGCGCGGGCG > NC_002947/5988668‑5988957 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |