Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 6,004,526 G→T 6.2% G152V (GGC→GTC) ‡ PP_5259 → LysR family transcriptional regulator

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029476,004,5260GT 6.2% 206.9 / 3.9 64G152V (GGC→GTC) ‡PP_5259LysR family transcriptional regulator
Reads supporting (aligned to +/- strand):  ref base G (31/29);  new base T (2/2);  total (33/31)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.09e-01

CCGAGTTTTGGCGTGCGCTGGTTGATCCCGCACCTCAAGGGGTTTGGCAAACGGCACGGCAATATCCACCTGGACATTCGCAACGAGATGGAGCCGTTCGCCCTGCTGCAAGGCTCGGCTGACGTGGTGTTCTTCTATGGCCAGGGCACCTGGCCGGGGGCCACTTGCGTGGAGTTGTTTCGCGAGGAGGTGGTGCCGGTGTGTGCGCCAGAGCTGCTGGCCGGGCGCAAGCTGGCCGGTGCCGAGGAGCTGGCCGAGCTGGTGCTGCTGCAAAGCACCTCGCGCCCGGAGGC  >  NC_002947/6004381‑6004673
                                                                                                                                                 |                                                                                                                                                   
ccGAGTTTTGGCGTGCGCTGGTTGATCCCGCACCTCAAGGGGTTTGGCAAACGGCACGGCAATATCCACCTGGACATTCGCAACGAGATGGAGCCGTTCGCCCTGCTGCAAGGCTCGGCTGACGTGGTGTTCTTCTATGGCCAGGGCAc                                                                                                                                                  >  4:168975/1‑149 (MQ=255)
        tGGCGTGCGCTGGTTGATCCCGCACCTCAAGGGGTTTGGCAAACGGCACGGCAATATCCACCTGGACATTCGCAACGAGATGGAGCCGTTCGCCCTGCTGCAAGGCTCGGCTGACGTGGTGTTCTTCTATGGCCAGGTGCCCTGGcca                                                                                                                                           <  6:363441/148‑2 (MQ=255)
         ggCGTGCGCTGGTTGATCCCGCACCTCAAGGGGTTTGGCAAACGGCACGGCAATATCCACCTGGACATTCGCAACGAGATGGAGCCGTTCGCCCTGCTGCAAGGCTCGGCTGACGTGGTGTTCTTCTATGGCCAGGGCACCTGGCCgg                                                                                                                                          <  1:229211/148‑1 (MQ=255)
          gCGTGCGCTGGTTGATCCCGCACCTCAAGGGGTTTGGCAAACGGCACGGCAATATCCACCTGGACATTCGCAACGAGATGGAGCCGTTCGCCCTGCTGCAAGGCTCGGCTGACGTGGTGTTCTTCTATGGCCAGGGCACCTGGCCgggg                                                                                                                                        <  3:64137/149‑1 (MQ=255)
          gCGTGCGCTGGTTGATCCCGCACCTCAAGGGGTTTGGCAAACGGCACGGCAATATCCACCTGGACATTCGCAACGAGATGGAGCCGTTCGCCCTGCTGCAAGGCTCGGCTGACGTGGTGTTCTTCTATGGCCAGGGCACCTGGCCgggg                                                                                                                                        <  8:107890/149‑1 (MQ=255)
                gcTGGTTGATCCCGCACCTCAAGGGGTTTGGCAAACGGCACGGCAATATCCACCTGGACATTCGCAACGAGATGGAGCCGTTCGCCCTGCTGCAAGGCTCGGCTGACGTGGTGTTCTTCTATGGCCAGGGCACCTGGCCGGGGGCCACt                                                                                                                                  >  7:212820/1‑149 (MQ=255)
                     ttGATCCCGCACCTCAAGGGGTTTGGCAAACGGCACGGCAATATCCACCTGGACATTCGCAACGAGATGGAGCCGTTCGCCCTGCTGCAAGGCTCGGCTGACGTGGTGTTCTTCTATGGCCAGGGCACCTGGCCGGGGGCCACTTGCGt                                                                                                                             <  5:45434/149‑1 (MQ=255)
                     ttGATCCCGCACCTCAAGGGGTTTGGCAAACGGCACGGCAATATCCACCTGGACATTCGCAACGAGATGGAGCCGTTCGCCCTGCTGCAAGGCTCGGCTGACGTGGTGTTCTTCTATGGCCAGGGCACCTGGCCGGGGGCCACTTGCGt                                                                                                                             >  7:20318/1‑149 (MQ=255)
                        aTCCCGCACCTCAAGGGGTTTGGCAAACGGCACGGCAATATCCACCTGGACATTCGCAACGAGATGGAGCCGTTCGCCCTGCTGCAAGGCTCGGCTGACGTGGTGTTCTTCTATGGCCAGGGCACCTGGCCGGGGGCCACTTGCGTGGa                                                                                                                          <  3:168975/149‑1 (MQ=255)
                           ccGCACCTCAAGGGGTTTGGCAAACGGCACGGCAATATCCACCTGGACATTCGCAACGAGATGGAGCCGTTCGCCCTGCTGCAAGGCTCGGCTGACGTGGTGTTCCTCTATGGCCAGGGCACCTGGCCGGGGGCCACTTGCGTGGAgtt                                                                                                                       >  4:38419/1‑149 (MQ=255)
                                ccTCAAGGGGTTTGGCAAACGGCACGGCAATATCCACGTGGACATTCGCAACGAGATGGAGCCGTTCGCCCTGCTGCAAGGCTCGGCTGACGTGGTGTTCTTCTATTGCCAGGGCACCTGGCCGGGGGCCACTTGCGTGGAGTTGTTTc                                                                                                                  <  3:111143/149‑1 (MQ=255)
                                  tCAAGGGGTTTGGCAAACGGCACGGCAATATCCACCTGGACATTCGCAACGAGATGGAGCCGTTCGCCCTGCTGCAAGGCTCGGCTGACGTGGTGTTCTTCTATGGCCAGGGCACCTGGCCGGGGGCCACTTGCGTGGAGTTGTTTcgc                                                                                                                >  4:108869/1‑149 (MQ=255)
                                    aaGGGGTTTGGCAAACGGCACGGCAATATCCACCTGGACATTCGCAACGAGATGGAGCCGTTCGCCCTGCTGCAAGGCTCGGCTGACGTGGTGTTCTTCTATGGCCAGGGCACCTGGCCGGGGGCCACTTGCGTGGAgtt                                                                                                                       <  4:79077/140‑1 (MQ=255)
                                    aaGGGGTTTGGCAAACGGCACGGCAATATCCACCTGGACATTCGCAACGAGATGGAGCCGTTCGCCCTGCTGCAAGGCTCGGCTGACGTGGTGTTCTTCTATGGCCAGGGCACCTGGCCGGGGGCCACTTGCGTGGAgtt                                                                                                                       >  3:79077/1‑140 (MQ=255)
                                    aaGGGGTTTGGCAAACGGCACGGCAATATCCACCTGGACATTCGCAACGAGATGGAGCCGTTCGCCCTGCTGCAAGGCTCGGCTGACGTGGTGTTCTTCTATGGCCAGGGCACCTGGCCGGGGGCCACTTGCGTGGAGTTGTTTcgc                                                                                                                <  5:36693/147‑1 (MQ=255)
                                    aaGGGGTTTGGCAAACGGCACGGCAATATCCACCTGGACATTCGCAACGAGATGGAGCCGTTCGCCCTGCTGCAAGGCTCGGCTGACGTGGTGTTCTTCTATGGCCAGGGCACCTGGCCGGGGGCCACTTGCGTGGAGTTGTTTcgc                                                                                                                >  6:191952/1‑147 (MQ=255)
                                    aaGGGGTTTGGCAAACGGCACGGCAATATCCACCTGGACATTCGCAACGAGATGGAGCCGTTCGCCCTGCTGCAAGGCTCGGCTGACGTGGTGTTCTTCTATGGCCAGGGCACCTGGCCGGGGGCCACTTGCGTGGAGTTGTTTCGCga                                                                                                              <  8:212820/149‑1 (MQ=255)
                                      ggggTTTGGCAAACGGCACGGCAATATCCACCTGGACATTCGCAACGAGATGGAGCCGTTCGCCCTGCTGCAAGGCTCGGCTGACGTGGTGTTCTTCTATGGCCAGGTGCCCTGGcca                                                                                                                                           >  1:153288/1‑117 (MQ=255)
                                      ggggTTTGGCAAACGGCACGGCAATATCCACCTGGACATTCGCAACGAGATGGAGCCGTTCGCCCTGCTGCAAGGCTCGGCTGACGTGGTGTTCTTCTATGGCCAGGTGCCCTGGcca                                                                                                                                           <  2:153288/118‑2 (MQ=255)
                                                aaaCGGCACGGCAATATCCACCTGGACATTCGCAACGAGATGGAGCCGTTCGCCCTGCTGCAAGGCTCGGCTGACGTGGTGTTCTTCTATGGCCAGGGCACCTGGCCGGGGGCCACTTGCGTGGAGTTGTTTCGCGAGGAGGTGGTGcc                                                                                                  >  4:228165/1‑149 (MQ=255)
                                                 aaCGGCACGGCAATATCCACCTGGACATTCGCAACGAGATGGAGCCGTTCGCCCTGCTGCAAGGCTCGGCTGACGTGGTGTTCTTCTATGGCCAGGGCACCTGGCCGGGGGCCACTTGCGTGGAGTTGTTTCGCGAGGAGGGGGTGCCg                                                                                                 <  8:20318/149‑1 (MQ=255)
                                                  acggcacggcAATATCCACCTGGACATTCGCAACGAGATGGAGCCGTTCGCCCTGCTGCAAGGCTCGGCTGACGTGGTGTTCTTCTATGGCCAGGGCACCTGGCCGGGGGCCACTTGCGTGGAGTTGTTTCGCGAGGAGGTGGTGCCgg                                                                                                >  4:159144/1‑149 (MQ=255)
                                                   cggcacggcaATATCCACCTGGACATTCGCAACGAGATGGAGCCGTTCGCCCTGCTGCAAGGCTCGGCTGACGTGGTGTTCTTCTATGGCCAGGGCACCTGGCCGGGGGCCACTTGCGTGGAGTTGTTTCGCGAGGAGGTGGTGCCGgt                                                                                               <  3:159144/149‑1 (MQ=255)
                                                    ggcacggcaATATCCACCTGGACATTCGCATCGAGATGGAGCCGTTCGCCCTGCTGCAAGGCTCGGCTGACGTGGTGTTCTTCTATGGCCAGGGCACCTGGCCGGGGGCCACTTGCGTGGAGTTGTTTCGCGAGGAGGTGGTGCCGgtg                                                                                              >  1:28983/1‑149 (MQ=255)
                                                         ggcaATATCCACCTGGACATTCGCAACGAGATGGAGCCGTTCGCCCTGCTGCAAGGCTCGGCTGACGTGGTGTTCTTCTATGGCCAGGGCACCTGGCCGGGGGCCACTTGCGTGGAGTTGTTTCGCGAGGAGGTGGTGCCGgt                                                                                               >  1:19605/1‑143 (MQ=255)
                                                         ggcaATATCCACCTGGACATTCGCAACGAGATGGAGCCGTTCGCCCTGCTGCAAGGCTCGGCTGACGTGGTGTTCTTCTATGGCCAGGGCACCTGGCCGGGGGCCACTTGCGTGGAGTTGTTTCGCGAGGAGGTGGTGCCGgt                                                                                               <  2:19605/143‑1 (MQ=255)
                                                           caATATCCACCTGGACATTCGCAACGAGATGGAGCCGTTCGCCCTGCTGCAAGGCTCGGCTGACGTGGTGTTCTTCTATGGCCAGGGCACCTGGCCGGGGGCCACTTGCGTGGAGTTGTTTCGCgag                                                                                                             <  3:232465/127‑1 (MQ=255)
                                                           caATATCCACCTGGACATTCGCAACGAGATGGAGCCGTTCGCCCTGCTGCAAGGCTCGGCTGACGTGGTGTTCTTCTATGGCCAGGGCACCTGGCCGGGGGCCACTTGCGTGGAGTTGTTTCGCgag                                                                                                             >  4:232465/1‑127 (MQ=255)
                                                                tCCACCTGGACATTCGCAACGAGATGGAGCCGTTCGCCCTGCTGCAAGGCTCGGCTGACGTGGTGCTCTTCTATGGCCAGGGCACCTGGCCGGGGGCCACTTGCGTGGAGTTGTTTCGCGAGGAGGTGGTGCCGgtgt                                                                                             <  3:319677/138‑1 (MQ=255)
                                                                tCCACCTGGACATTCGCAACGAGATGGAGCCGTTCGCCCTGCTGCAAGGCTCGGCTGACGTGGTGCTCTTCTATGGCCAGGGCACCTGGCCGGGGGCCACTTGCGTGGAGTTGTTTCGCGAGGAGGTGGTGCCGgtgt                                                                                             >  4:319677/1‑138 (MQ=255)
                                                                    ccTGGACATTCGCAACGAGATGGAGCCGTTCGCCCTGCTGCAAGGCTCGGCTGACGTGGTGTTCTTCTATGGCCAGGGCACCTGGCCGGGGGCCACTTGCGTGGAGTTGTTTCGCGAGGAGGTGGTGCCGGTGTGTGCGCCAGAgctgc                                                                              >  7:60976/1‑149 (MQ=255)
                                                                     cTGGACATTCGCAACGAGATGGAGCCGTTCGCCCTGCTGCAAGGCTCGGCTGACGTGGTGTTCTTCTATGGCCAGGGCACCTGGCCGGGGGCCACTTGCGTGGa                                                                                                                          <  8:164487/104‑1 (MQ=255)
                                                                     cTGGACATTCGCAACGAGATGGAGCCGTTCGCCCTGCTGCAAGGCTCGGCTGACGTGGTGTTCTTCTATGGCCAGGGCACCTGGCCGGGGGCCACTTGCGTGGa                                                                                                                          >  7:164487/1‑104 (MQ=255)
                                                                          cATTCGCAACGAGATGGAGCCGTTCGCCCTGCTGCAAGGCTCGGCTGACGTGGTGTTCTTCTATGGCCAGGGCACCTGGCCGGGGGCCACTTGCGTGGAGTTGTTTCGCGAGGAGGTGGTGCCGGTGTGTGCGCCAGAGCTGCTGGCCg                                                                        <  2:28983/149‑1 (MQ=255)
                                                                          cATTCGCAACGAGATGGAGCCGTTCGCCCTGCTGCAAGGCTCGGCTGACGTGGTGTTCTTCTATGGCCAGGGCACCTGGCCGGGGGCCACTTGCGTGGAGTTGTTTCGCGAGGAGGTGGTGCCGGTGTGTGCGCCAGAGCTGCTGGCCg                                                                        <  7:19058/149‑1 (MQ=255)
                                                                          cATTCGCAACGAGAGGGAGCCGTTCGCCCTGCTGCAAGGCTCGGCTGTCGGGGTGTTCTTCTCTGGCCAGGGCACCTGGCCGGGGGCCACTTGCGTGGAGTTGTTTCGCGAGGAGGTGGTGCCGGTGTGTGCGCCAGAGCTGCTGGCCg                                                                        <  4:184373/149‑1 (MQ=255)
                                                                                cAACGAGATGGAGCCGTTCGCCCTGCTGCAAGGCTCGGCTGACGTGGTGTTCTTCTATGGCCAGGGCACCTGGCCGGGGGCCACTTGCGTGGAGTTGTTTCGCGAGGAGGTGGTGCCGGTGTGTGCGCCa                                                                                     <  7:108068/130‑1 (MQ=255)
                                                                                cAACGAGATGGAGCCGTTCGCCCTGCTGCAAGGCTCGGCTGACGTGGTGTTCTTCTATGGCCAGGGCACCTGGCCGGGGGCCACTTGCGTGGAGTTGTTTCGCGAGGAGGTGGTGCCGGTGTGTGCGCCa                                                                                     >  8:108068/1‑130 (MQ=255)
                                                                                       aTGGAGCCGTTCGCCCTGCTGCAAGGCTCGGCTGACGTGGTGTTCTTCTATGGCCAGGGCACCTGGCCGGGGGCCACTTGCGTGGAGTTGTTTCGCGAGGAGGTGGTGCCGGTGTGTGCGCCAGAGCTGCTGGCCGGGCGCAAGCTg                                                             >  7:238860/1‑147 (MQ=255)
                                                                                       aTGGAGCCGTTCGCCCTGCTGCAAGGCTCGGCTGACGTGGTGTTCTTCTATGGCCAGGGCACCTGGCCGGGGGCCACTTGCGTGGAGTTGTTTCGCGAGGAGGTGGTGCCGGTGTGTGCGCCAGAGCTGCTGGCCGGGCGCAAGCTg                                                             <  8:238860/147‑1 (MQ=255)
                                                                                        tGGAGCCGTTCGCCCTGCTGCAAGGCTCGGCTGACGTGGTGTTCTTCTATGGCCAGGGCACCTGGCCGGGGGCCACTTGCGTGGAGTTGTTTCGCGAGGAGGTGGTGCCGGTGTGTGCGCCAGAGCTGCTGGCCGGGCGCAAGCTGGcc                                                          <  5:191952/149‑1 (MQ=255)
                                                                                          gAGCCGTTCGCCCTGCTGCAAGGCTCGGCTGACGTGGTGTTCTTCTATGGCCAGGGCACCTGGCCGGGGGCCACTTGCGTGGAGTTGTTTCGCGAGGAGGTGGTGCCGGTGTGTGCGCCAGAGCTGCTGGCCGGGCGCAAGCTGGCCgg                                                        >  4:201124/1‑149 (MQ=255)
                                                                                                   gCCCTGCTGCAAGGCTCGGCTGACGTGGTGTTCCTCTATGGCCAGGGCACCTGGCCGGGGGCCACTTGCGTGGAGTTGTTTCGCGAGGAGGTGGTGCCGGTGTGTGCGCCAGAGCTGCTGGCCGGGCGCAAGCTGGCCGGTGCCgagga                                               <  3:38419/149‑1 (MQ=255)
                                                                                                     ccTGCTGCAAGGCTCGGCTGACGTGGTGTTCTTCTATGGCCAGGGCACCTGGCCGGGGGCCACTTGCGTGGAGTTGTTTCGCGAGGAGGTGGTGCCGGTGTGTGCGCCAGAGCTGCTGGCCGGGCGCAAGCTGGCCGGTGCCGAGGAGc                                             >  5:177210/1‑149 (MQ=255)
                                                                                                     ccTGCTGCAAGGCTCGGCTGACGTGGTGTTCTTCTATGGCCAGGGCACCTGGCCGGGGGCCACTTGCGTGGAGTTGTTTCGCGAGGAGGTGGTGCCGGTGTGTGCGCCAGAGCTGCTGGCCGGGCGCAAGCTGGCCGGTGCCGAGGAGc                                             >  2:223221/1‑149 (MQ=255)
                                                                                                          tgcAAGGCTCGGCTGACGTGGTGTTCTTCTATGGCCAGGGCACCTGGCCGGGGGCCACTTGCGTGGAGTTGTTTCGCGAGGAGGTGGTGCCGGTGTGTGCGCCAGAGCTGCTGGCCGGGCGCAAGCTGGCCGGTGCCGAGGAGCTGGcc                                        >  2:39561/1‑149 (MQ=255)
                                                                                                            cAAGGCTCGGCTGACGTGGTGTTCTTCTATGGCCAGGGCACCTGGCCGGGGGCCACTTGCGTGGAGTTGTTTCGCGAGGATGTGGTGCCGGTGTGTGCGCCAGAGCTGCTGGCCGGGCGCAAGCTGGCCGGCGCCGAGGCGCTGGcc                                        >  7:112188/1‑147 (MQ=255)
                                                                                                            cAAGGCTCGGCTGACGTGGTGTTCTTCTATGGCCAGGGCACCTGGCCGGGGGCCACTTGCGTGGAGTTGTTTCGCGAGGAGGTGGTGCCGGTGTGTGCGCCAGAGCTGCTGGCCGGGCGCAAGCTGGCCGGTGCCGAGGAGCTGGcc                                        >  8:82235/1‑147 (MQ=255)
                                                                                                            cAAGGCTCGGCTGACGTGGTGTTCTTCTATGGCCAGGGCACCTGGCCGGGGGCCACTTGCGTGGAGTTGTTTCGCGAGGAGGTGGTGCCGGTGTGTGCGCCAGAGCTGCTGGCCGGGCGCAAGCTGGCCGGTGCCGAGGAGCTGGcc                                        >  3:115501/1‑147 (MQ=255)
                                                                                                              aGGCTCGGCTGACGTGGTGTTCTTCTATGGCCAGGGCACCTGGCCGGGGGCCACTTGCGTGGAGTTGTTTCGCGAGGAGGTGGTGCCGGTGTGTGCGCCAGAGCTGCTGGCCGGGCGCAAGCTGGCCGGTGCCGAGGAGCTGGCCGAGc                                    >  2:217310/1‑149 (MQ=255)
                                                                                                                 cTCGGCTGACGTGGTGTTCTTCTATGGCCAGGGCACCTGGCCGGGGGCCACTTGCGTGGAGTTGTTTCGCGAGGAGGTGGTGCCGGTGTGTGCGCCAGAGCTGCTGGCCGGGCGCAAGCTGGCCGGTGCCGAGGAGCTGGCCGAGCTgg                                 <  8:60976/149‑1 (MQ=255)
                                                                                                                    ggCTGACGTGGTGTTCTTCTATGGCCAGGGCACCTGGCCGGGGGCCACTTGCGTGGAGTTGTTTCGCGAGGAGGTGGTGCCGGTGTGTGCGCCAGAGCTGCTGGCCGGGCGCAAGCTGGCCGGTGCCGAGGAGCTGGCCGAGCTGGtgc                              >  6:71698/1‑149 (MQ=255)
                                                                                                                           gtggtgTTCTTCTATGGCCAGGGCACCTGGCCGGGGGCCACTTGCGTGGAGTTGTTTCGCGAGGAGGTGGTGCCGGTGTGTGCGCCAGAGCTGCTGGCCGGGCGCAAGCTGGCCGGTGCCGAGGAGCTGGCCGAGCTGGtgctgctgc                        >  2:198671/1‑148 (MQ=255)
                                                                                                                             ggtgTTCTTCTATGGCCAGGGCACCTGGCCGGGGGCCACTTGCGTGGTGTTGTTTCGCGAGGAGGTGGTGCCGGTGTGTGCGCCAGAGCTGCTGGCCGGGCGCAAGCTGGCCGGTGCCGAGGAGCTGGCCGAGCTGGTGCTGCTGCa                       >  2:93836/1‑147 (MQ=255)
                                                                                                                               tgtTCTTCTATGGCCAGGGCACCTGGCCGGGGGCCACTTGCGTGGAGTTGTTTCGCGAGGAGGTGGTGCCGGTGTGTGCGCCAGAGCTGCTGGCCGGGCGCAAGCTGGCCGGTGCCGAGGAGCTGGCCGAGCTGGTGCTGCTGCAAAGc                   <  3:228165/149‑1 (MQ=255)
                                                                                                                                 ttcttcTATGGCCAGGGCACCTGGCCGGGGGCCACTTGCGTGGAGTTGTTTCGCGAGGAGGTGGTGCCGGTGTGTGCGCCAGAGCTGCTGGCCGGGCGCAAGCTGGCCGGTGCCGAGGAGCTGGCCGAGCTGGTGCTGCTGCAAAGCAc                 >  3:263741/1‑149 (MQ=255)
                                                                                                                                 ttcttcTATGGCCAGGGCACCTGGCCGGGGGCCACTTGCGTGGAGTTGTTTCGCGAGGAGGTGGTGCCGGTGTGTGCGCCAGAGCTGCTGGCCGGGCGCAAGCTGGCCGGTGCCGAGGAGCTGGCCGAGCTGGTGCTGCTGCAAAGCAc                 <  1:223221/149‑1 (MQ=255)
                                                                                                                                 ttcttcTATGGCCAGGGCACCTGGCCGGGGGCCACTTGCGTGGAGTTGTTTCGCGAGGAGGTGGTGCCGGTGTGTGCGCCAGAGCTGCTGGCCGGGCGCAAGCTGGCCGGTGCCGAGGAGCTGGCCGAGCTGGTGCTGCTGCAAAGCAc                 <  6:177210/149‑1 (MQ=255)
                                                                                                                                      ctATGGCCAGGGCACCTGGCCGGGGGCCACTTGCGTGGAGTTGTTTCGCGAGGAGGTGGTGCCGGTGTGTGCGCCAGAGCTGCTGGCCGGGCGCAAGCAGGCCGGTGCCGAGGAGCTGGCCGAGCTGGTGCTGCTGCAAAGCACCTcgc            >  2:142962/1‑149 (MQ=255)
                                                                                                                                      ccccggCCAGGTGCCCTGGCCAGGGGCCACTTGCGTGGAGTTGTTTCGCGAGGAGGTGGTGCCGGTGTGTGCGCCAGAGCTGCTGGCCGGGCGCAAGCTGGCCGGTGCCGAGGAGCTGGCCGCGCTGGTGCTGCTGCAAAGCACCTcgc            >  7:176372/5‑149 (MQ=255)
                                                                                                                                             cAGGGCACCTGGCCGGGGGCCACTTGCGTGGAGTTGTTTCGCGAGGAGGTGGTGCCGGTGTGTGCGCCAGAGCTGCTGGCCGGGCGCAAGCTGGCCGGTGCCGAGGAGCTGGCCGAGCTGGTGCTGCTGCAAAGCACCTCGCGCCCGGa     <  7:82235/149‑1 (MQ=255)
                                                                                                                                             cAGGGCACCTGGCCGGGGGCCACTTGCGTGGAGTTGTTTCGCGAGGAGGTGGTGCCGGTGTGTGCGCCAGAGCTGCTGGCCGGGCGCAAGCTGGCCGGTGCCGAGGAGCTGGCCGAGCTGGTGCTGCTGCAAAGCACCTCGCGCCCGGa     <  4:115501/149‑1 (MQ=255)
                                                                                                                                             cAGGGCACCTGGCCGGGGGCCACTTGCGTGGAGTTGTTTCGCGAGGAGGTGGTGCCGGTGTGGGCGCCAGAGCTGCTGGCCGGGCGCAAGCTGGCCGGTGCCGAGGAGCTGGCCGAGCTg                                  <  2:246456/120‑1 (MQ=255)
                                                                                                                                             cAGGGCACCTGGCCGGGGGCCACTTGCGTGGAGTTGTTTCGCGAGCAGGTGGTGCCGGTGTGTGCGCCAGAGCTGCTGGCCGGGCGCAAGCTGGCCGGTGCCGAGGAGCTGGCCGAGCTg                                  >  1:246456/1‑120 (MQ=255)
                                                                                                                                                ggCACCTGGCCGGGGGCCACTTGCGTGGAGTTGTTTCGCGAGGAGGTGGTGCCGGTGTGTGCGCCAGAGCTGCTGGCCGGGCGCAAGCTGGCCGGTGCCGAGGAGCTGGCCGAGCTGGTGCTGCTGCAAAGCACCTCGCGCCCGGAGGc  >  6:77892/1‑149 (MQ=255)
                                                                                                                                                 |                                                                                                                                                   
CCGAGTTTTGGCGTGCGCTGGTTGATCCCGCACCTCAAGGGGTTTGGCAAACGGCACGGCAATATCCACCTGGACATTCGCAACGAGATGGAGCCGTTCGCCCTGCTGCAAGGCTCGGCTGACGTGGTGTTCTTCTATGGCCAGGGCACCTGGCCGGGGGCCACTTGCGTGGAGTTGTTTCGCGAGGAGGTGGTGCCGGTGTGTGCGCCAGAGCTGCTGGCCGGGCGCAAGCTGGCCGGTGCCGAGGAGCTGGCCGAGCTGGTGCTGCTGCAAAGCACCTCGCGCCCGGAGGC  >  NC_002947/6004381‑6004673

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑