Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 6,004,528 A→C 6.1% T153P (ACC→CCC)  PP_5259 → LysR family transcriptional regulator

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029476,004,5280AC 6.1% 206.5 / 3.7 66T153P (ACC→CCC) PP_5259LysR family transcriptional regulator
Reads supporting (aligned to +/- strand):  ref base A (33/29);  new base C (2/2);  total (35/31)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.32e-01

CCGAGTTTTGGCGTGCGCTGGTTGATCCCGCACCTCAAGGGGTTTGGCAAACGGCACGGCAATATCCACCTGGACATTCGCAACGAGATGGAGCCGTTCGCCCTGCTGCAAGGCTCGGCTGACGTGGTGTTCTTCTATGGCCAGGGCACCTGGCCGGGGGCCACTTGCGTGGAGTTGTTTCGCGAGGAGGTGGTGCCGGTGTGTGCGCCAGAGCTGCTGGCCGGGCGCAAGCTGGCCGGTGCCGAGGAGCTGGCCGAGCTGGTGCTGCTGCAAAGCACCTCGCGCCCGGAGGCCT  >  NC_002947/6004381‑6004675
                                                                                                                                                   |                                                                                                                                                   
ccGAGTTTTGGCGTGCGCTGGTTGATCCCGCACCTCAAGGGGTTTGGCAAACGGCACGGCAATATCCACCTGGACATTCGCAACGAGATGGAGCCGTTCGCCCTGCTGCAAGGCTCGGCTGACGTGGTGTTCTTCTATGGCCAGGGCAc                                                                                                                                                    >  4:168975/1‑149 (MQ=255)
        tGGCGTGCGCTGGTTGATCCCGCACCTCAAGGGGTTTGGCAAACGGCACGGCAATATCCACCTGGACATTCGCAACGAGATGGAGCCGTTCGCCCTGCTGCAAGGCTCGGCTGACGTGGTGTTCTTCTATGGCCAGGTGCCCTGGcca                                                                                                                                             <  6:363441/148‑2 (MQ=255)
         ggCGTGCGCTGGTTGATCCCGCACCTCAAGGGGTTTGGCAAACGGCACGGCAATATCCACCTGGACATTCGCAACGAGATGGAGCCGTTCGCCCTGCTGCAAGGCTCGGCTGACGTGGTGTTCTTCTATGGCCAGGGCACCTGGCCgg                                                                                                                                            <  1:229211/148‑1 (MQ=255)
          gCGTGCGCTGGTTGATCCCGCACCTCAAGGGGTTTGGCAAACGGCACGGCAATATCCACCTGGACATTCGCAACGAGATGGAGCCGTTCGCCCTGCTGCAAGGCTCGGCTGACGTGGTGTTCTTCTATGGCCAGGGCACCTGGCCgggg                                                                                                                                          <  3:64137/149‑1 (MQ=255)
          gCGTGCGCTGGTTGATCCCGCACCTCAAGGGGTTTGGCAAACGGCACGGCAATATCCACCTGGACATTCGCAACGAGATGGAGCCGTTCGCCCTGCTGCAAGGCTCGGCTGACGTGGTGTTCTTCTATGGCCAGGGCACCTGGCCgggg                                                                                                                                          <  8:107890/149‑1 (MQ=255)
                gcTGGTTGATCCCGCACCTCAAGGGGTTTGGCAAACGGCACGGCAATATCCACCTGGACATTCGCAACGAGATGGAGCCGTTCGCCCTGCTGCAAGGCTCGGCTGACGTGGTGTTCTTCTATGGCCAGGGCACCTGGCCGGGGGCCACt                                                                                                                                    >  7:212820/1‑149 (MQ=255)
                     ttGATCCCGCACCTCAAGGGGTTTGGCAAACGGCACGGCAATATCCACCTGGACATTCGCAACGAGATGGAGCCGTTCGCCCTGCTGCAAGGCTCGGCTGACGTGGTGTTCTTCTATGGCCAGGGCACCTGGCCGGGGGCCACTTGCGt                                                                                                                               <  5:45434/149‑1 (MQ=255)
                     ttGATCCCGCACCTCAAGGGGTTTGGCAAACGGCACGGCAATATCCACCTGGACATTCGCAACGAGATGGAGCCGTTCGCCCTGCTGCAAGGCTCGGCTGACGTGGTGTTCTTCTATGGCCAGGGCACCTGGCCGGGGGCCACTTGCGt                                                                                                                               >  7:20318/1‑149 (MQ=255)
                        aTCCCGCACCTCAAGGGGTTTGGCAAACGGCACGGCAATATCCACCTGGACATTCGCAACGAGATGGAGCCGTTCGCCCTGCTGCAAGGCTCGGCTGACGTGGTGTTCTTCTATGGCCAGGGCACCTGGCCGGGGGCCACTTGCGTGGa                                                                                                                            <  3:168975/149‑1 (MQ=255)
                           ccGCACCTCAAGGGGTTTGGCAAACGGCACGGCAATATCCACCTGGACATTCGCAACGAGATGGAGCCGTTCGCCCTGCTGCAAGGCTCGGCTGACGTGGTGTTCCTCTATGGCCAGGGCACCTGGCCGGGGGCCACTTGCGTGGAgtt                                                                                                                         >  4:38419/1‑149 (MQ=255)
                                ccTCAAGGGGTTTGGCAAACGGCACGGCAATATCCACGTGGACATTCGCAACGAGATGGAGCCGTTCGCCCTGCTGCAAGGCTCGGCTGACGTGGTGTTCTTCTATTGCCAGGGCACCTGGCCGGGGGCCACTTGCGTGGAGTTGTTTc                                                                                                                    <  3:111143/149‑1 (MQ=255)
                                  tCAAGGGGTTTGGCAAACGGCACGGCAATATCCACCTGGACATTCGCAACGAGATGGAGCCGTTCGCCCTGCTGCAAGGCTCGGCTGACGTGGTGTTCTTCTATGGCCAGGGCACCTGGCCGGGGGCCACTTGCGTGGAGTTGTTTcgc                                                                                                                  >  4:108869/1‑149 (MQ=255)
                                    aaGGGGTTTGGCAAACGGCACGGCAATATCCACCTGGACATTCGCAACGAGATGGAGCCGTTCGCCCTGCTGCAAGGCTCGGCTGACGTGGTGTTCTTCTATGGCCAGGGCACCTGGCCGGGGGCCACTTGCGTGGAgtt                                                                                                                         >  3:79077/1‑140 (MQ=255)
                                    aaGGGGTTTGGCAAACGGCACGGCAATATCCACCTGGACATTCGCAACGAGATGGAGCCGTTCGCCCTGCTGCAAGGCTCGGCTGACGTGGTGTTCTTCTATGGCCAGGGCACCTGGCCGGGGGCCACTTGCGTGGAgtt                                                                                                                         <  4:79077/140‑1 (MQ=255)
                                    aaGGGGTTTGGCAAACGGCACGGCAATATCCACCTGGACATTCGCAACGAGATGGAGCCGTTCGCCCTGCTGCAAGGCTCGGCTGACGTGGTGTTCTTCTATGGCCAGGGCACCTGGCCGGGGGCCACTTGCGTGGAGTTGTTTcgc                                                                                                                  >  6:191952/1‑147 (MQ=255)
                                    aaGGGGTTTGGCAAACGGCACGGCAATATCCACCTGGACATTCGCAACGAGATGGAGCCGTTCGCCCTGCTGCAAGGCTCGGCTGACGTGGTGTTCTTCTATGGCCAGGGCACCTGGCCGGGGGCCACTTGCGTGGAGTTGTTTcgc                                                                                                                  <  5:36693/147‑1 (MQ=255)
                                    aaGGGGTTTGGCAAACGGCACGGCAATATCCACCTGGACATTCGCAACGAGATGGAGCCGTTCGCCCTGCTGCAAGGCTCGGCTGACGTGGTGTTCTTCTATGGCCAGGGCACCTGGCCGGGGGCCACTTGCGTGGAGTTGTTTCGCga                                                                                                                <  8:212820/149‑1 (MQ=255)
                                      ggggTTTGGCAAACGGCACGGCAATATCCACCTGGACATTCGCAACGAGATGGAGCCGTTCGCCCTGCTGCAAGGCTCGGCTGACGTGGTGTTCTTCTATGGCCAGGTGCCCTGGcca                                                                                                                                             >  1:153288/1‑117 (MQ=255)
                                      ggggTTTGGCAAACGGCACGGCAATATCCACCTGGACATTCGCAACGAGATGGAGCCGTTCGCCCTGCTGCAAGGCTCGGCTGACGTGGTGTTCTTCTATGGCCAGGTGCCCTGGcca                                                                                                                                             <  2:153288/118‑2 (MQ=255)
                                                aaaCGGCACGGCAATATCCACCTGGACATTCGCAACGAGATGGAGCCGTTCGCCCTGCTGCAAGGCTCGGCTGACGTGGTGTTCTTCTATGGCCAGGGCACCTGGCCGGGGGCCACTTGCGTGGAGTTGTTTCGCGAGGAGGTGGTGcc                                                                                                    >  4:228165/1‑149 (MQ=255)
                                                 aaCGGCACGGCAATATCCACCTGGACATTCGCAACGAGATGGAGCCGTTCGCCCTGCTGCAAGGCTCGGCTGACGTGGTGTTCTTCTATGGCCAGGGCACCTGGCCGGGGGCCACTTGCGTGGAGTTGTTTCGCGAGGAGGGGGTGCCg                                                                                                   <  8:20318/149‑1 (MQ=255)
                                                  acggcacggcAATATCCACCTGGACATTCGCAACGAGATGGAGCCGTTCGCCCTGCTGCAAGGCTCGGCTGACGTGGTGTTCTTCTATGGCCAGGGCACCTGGCCGGGGGCCACTTGCGTGGAGTTGTTTCGCGAGGAGGTGGTGCCgg                                                                                                  >  4:159144/1‑149 (MQ=255)
                                                   cggcacggcaATATCCACCTGGACATTCGCAACGAGATGGAGCCGTTCGCCCTGCTGCAAGGCTCGGCTGACGTGGTGTTCTTCTATGGCCAGGGCACCTGGCCGGGGGCCACTTGCGTGGAGTTGTTTCGCGAGGAGGTGGTGCCGgt                                                                                                 <  3:159144/149‑1 (MQ=255)
                                                    ggcacggcaATATCCACCTGGACATTCGCATCGAGATGGAGCCGTTCGCCCTGCTGCAAGGCTCGGCTGACGTGGTGTTCTTCTATGGCCAGGGCACCTGGCCGGGGGCCACTTGCGTGGAGTTGTTTCGCGAGGAGGTGGTGCCGgtg                                                                                                >  1:28983/1‑149 (MQ=255)
                                                         ggcaATATCCACCTGGACATTCGCAACGAGATGGAGCCGTTCGCCCTGCTGCAAGGCTCGGCTGACGTGGTGTTCTTCTATGGCCAGGGCACCTGGCCGGGGGCCACTTGCGTGGAGTTGTTTCGCGAGGAGGTGGTGCCGgt                                                                                                 >  1:19605/1‑143 (MQ=255)
                                                         ggcaATATCCACCTGGACATTCGCAACGAGATGGAGCCGTTCGCCCTGCTGCAAGGCTCGGCTGACGTGGTGTTCTTCTATGGCCAGGGCACCTGGCCGGGGGCCACTTGCGTGGAGTTGTTTCGCGAGGAGGTGGTGCCGgt                                                                                                 <  2:19605/143‑1 (MQ=255)
                                                           caATATCCACCTGGACATTCGCAACGAGATGGAGCCGTTCGCCCTGCTGCAAGGCTCGGCTGACGTGGTGTTCTTCTATGGCCAGGGCACCTGGCCGGGGGCCACTTGCGTGGAGTTGTTTCGCgag                                                                                                               <  3:232465/127‑1 (MQ=255)
                                                           caATATCCACCTGGACATTCGCAACGAGATGGAGCCGTTCGCCCTGCTGCAAGGCTCGGCTGACGTGGTGTTCTTCTATGGCCAGGGCACCTGGCCGGGGGCCACTTGCGTGGAGTTGTTTCGCgag                                                                                                               >  4:232465/1‑127 (MQ=255)
                                                                tCCACCTGGACATTCGCAACGAGATGGAGCCGTTCGCCCTGCTGCAAGGCTCGGCTGACGTGGTGCTCTTCTATGGCCAGGGCACCTGGCCGGGGGCCACTTGCGTGGAGTTGTTTCGCGAGGAGGTGGTGCCGgtgt                                                                                               <  3:319677/138‑1 (MQ=255)
                                                                tCCACCTGGACATTCGCAACGAGATGGAGCCGTTCGCCCTGCTGCAAGGCTCGGCTGACGTGGTGCTCTTCTATGGCCAGGGCACCTGGCCGGGGGCCACTTGCGTGGAGTTGTTTCGCGAGGAGGTGGTGCCGgtgt                                                                                               >  4:319677/1‑138 (MQ=255)
                                                                    ccTGGACATTCGCAACGAGATGGAGCCGTTCGCCCTGCTGCAAGGCTCGGCTGACGTGGTGTTCTTCTATGGCCAGGGCACCTGGCCGGGGGCCACTTGCGTGGAGTTGTTTCGCGAGGAGGTGGTGCCGGTGTGTGCGCCAGAgctgc                                                                                >  7:60976/1‑149 (MQ=255)
                                                                     cTGGACATTCGCAACGAGATGGAGCCGTTCGCCCTGCTGCAAGGCTCGGCTGACGTGGTGTTCTTCTATGGCCAGGGCACCTGGCCGGGGGCCACTTGCGTGGa                                                                                                                            <  8:164487/104‑1 (MQ=255)
                                                                     cTGGACATTCGCAACGAGATGGAGCCGTTCGCCCTGCTGCAAGGCTCGGCTGACGTGGTGTTCTTCTATGGCCAGGGCACCTGGCCGGGGGCCACTTGCGTGGa                                                                                                                            >  7:164487/1‑104 (MQ=255)
                                                                          cATTCGCAACGAGATGGAGCCGTTCGCCCTGCTGCAAGGCTCGGCTGACGTGGTGTTCTTCTATGGCCAGGGCACCTGGCCGGGGGCCACTTGCGTGGAGTTGTTTCGCGAGGAGGTGGTGCCGGTGTGTGCGCCAGAGCTGCTGGCCg                                                                          <  7:19058/149‑1 (MQ=255)
                                                                          cATTCGCAACGAGATGGAGCCGTTCGCCCTGCTGCAAGGCTCGGCTGACGTGGTGTTCTTCTATGGCCAGGGCACCTGGCCGGGGGCCACTTGCGTGGAGTTGTTTCGCGAGGAGGTGGTGCCGGTGTGTGCGCCAGAGCTGCTGGCCg                                                                          <  2:28983/149‑1 (MQ=255)
                                                                          cATTCGCAACGAGAGGGAGCCGTTCGCCCTGCTGCAAGGCTCGGCTGTCGGGGTGTTCTTCTCTGGCCAGGGCACCTGGCCGGGGGCCACTTGCGTGGAGTTGTTTCGCGAGGAGGTGGTGCCGGTGTGTGCGCCAGAGCTGCTGGCCg                                                                          <  4:184373/149‑1 (MQ=255)
                                                                                cAACGAGATGGAGCCGTTCGCCCTGCTGCAAGGCTCGGCTGACGTGGTGTTCTTCTATGGCCAGGGCACCTGGCCGGGGGCCACTTGCGTGGAGTTGTTTCGCGAGGAGGTGGTGCCGGTGTGTGCGCCa                                                                                       <  7:108068/130‑1 (MQ=255)
                                                                                cAACGAGATGGAGCCGTTCGCCCTGCTGCAAGGCTCGGCTGACGTGGTGTTCTTCTATGGCCAGGGCACCTGGCCGGGGGCCACTTGCGTGGAGTTGTTTCGCGAGGAGGTGGTGCCGGTGTGTGCGCCa                                                                                       >  8:108068/1‑130 (MQ=255)
                                                                                       aTGGAGCCGTTCGCCCTGCTGCAAGGCTCGGCTGACGTGGTGTTCTTCTATGGCCAGGGCACCTGGCCGGGGGCCACTTGCGTGGAGTTGTTTCGCGAGGAGGTGGTGCCGGTGTGTGCGCCAGAGCTGCTGGCCGGGCGCAAGCTg                                                               <  8:238860/147‑1 (MQ=255)
                                                                                       aTGGAGCCGTTCGCCCTGCTGCAAGGCTCGGCTGACGTGGTGTTCTTCTATGGCCAGGGCACCTGGCCGGGGGCCACTTGCGTGGAGTTGTTTCGCGAGGAGGTGGTGCCGGTGTGTGCGCCAGAGCTGCTGGCCGGGCGCAAGCTg                                                               >  7:238860/1‑147 (MQ=255)
                                                                                        tGGAGCCGTTCGCCCTGCTGCAAGGCTCGGCTGACGTGGTGTTCTTCTATGGCCAGGGCACCTGGCCGGGGGCCACTTGCGTGGAGTTGTTTCGCGAGGAGGTGGTGCCGGTGTGTGCGCCAGAGCTGCTGGCCGGGCGCAAGCTGGcc                                                            <  5:191952/149‑1 (MQ=255)
                                                                                          gAGCCGTTCGCCCTGCTGCAAGGCTCGGCTGACGTGGTGTTCTTCTATGGCCAGGGCACCTGGCCGGGGGCCACTTGCGTGGAGTTGTTTCGCGAGGAGGTGGTGCCGGTGTGTGCGCCAGAGCTGCTGGCCGGGCGCAAGCTGGCCgg                                                          >  4:201124/1‑149 (MQ=255)
                                                                                                   gCCCTGCTGCAAGGCTCGGCTGACGTGGTGTTCCTCTATGGCCAGGGCACCTGGCCGGGGGCCACTTGCGTGGAGTTGTTTCGCGAGGAGGTGGTGCCGGTGTGTGCGCCAGAGCTGCTGGCCGGGCGCAAGCTGGCCGGTGCCgagga                                                 <  3:38419/149‑1 (MQ=255)
                                                                                                     ccTGCTGCAAGGCTCGGCTGACGTGGTGTTCTTCTATGGCCAGGGCACCTGGCCGGGGGCCACTTGCGTGGAGTTGTTTCGCGAGGAGGTGGTGCCGGTGTGTGCGCCAGAGCTGCTGGCCGGGCGCAAGCTGGCCGGTGCCGAGGAGc                                               >  5:177210/1‑149 (MQ=255)
                                                                                                     ccTGCTGCAAGGCTCGGCTGACGTGGTGTTCTTCTATGGCCAGGGCACCTGGCCGGGGGCCACTTGCGTGGAGTTGTTTCGCGAGGAGGTGGTGCCGGTGTGTGCGCCAGAGCTGCTGGCCGGGCGCAAGCTGGCCGGTGCCGAGGAGc                                               >  2:223221/1‑149 (MQ=255)
                                                                                                          tgcAAGGCTCGGCTGACGTGGTGTTCTTCTATGGCCAGGGCACCTGGCCGGGGGCCACTTGCGTGGAGTTGTTTCGCGAGGAGGTGGTGCCGGTGTGTGCGCCAGAGCTGCTGGCCGGGCGCAAGCTGGCCGGTGCCGAGGAGCTGGcc                                          >  2:39561/1‑149 (MQ=255)
                                                                                                            cAAGGCTCGGCTGACGTGGTGTTCTTCTATGGCCAGGGCACCTGGCCGGGGGCCACTTGCGTGGAGTTGTTTCGCGAGGATGTGGTGCCGGTGTGTGCGCCAGAGCTGCTGGCCGGGCGCAAGCTGGCCGGCGCCGAGGCGCTGGcc                                          >  7:112188/1‑147 (MQ=255)
                                                                                                            cAAGGCTCGGCTGACGTGGTGTTCTTCTATGGCCAGGGCACCTGGCCGGGGGCCACTTGCGTGGAGTTGTTTCGCGAGGAGGTGGTGCCGGTGTGTGCGCCAGAGCTGCTGGCCGGGCGCAAGCTGGCCGGTGCCGAGGAGCTGGcc                                          >  8:82235/1‑147 (MQ=255)
                                                                                                            cAAGGCTCGGCTGACGTGGTGTTCTTCTATGGCCAGGGCACCTGGCCGGGGGCCACTTGCGTGGAGTTGTTTCGCGAGGAGGTGGTGCCGGTGTGTGCGCCAGAGCTGCTGGCCGGGCGCAAGCTGGCCGGTGCCGAGGAGCTGGcc                                          >  3:115501/1‑147 (MQ=255)
                                                                                                              aGGCTCGGCTGACGTGGTGTTCTTCTATGGCCAGGGCACCTGGCCGGGGGCCACTTGCGTGGAGTTGTTTCGCGAGGAGGTGGTGCCGGTGTGTGCGCCAGAGCTGCTGGCCGGGCGCAAGCTGGCCGGTGCCGAGGAGCTGGCCGAGc                                      >  2:217310/1‑149 (MQ=255)
                                                                                                                 cTCGGCTGACGTGGTGTTCTTCTATGGCCAGGGCACCTGGCCGGGGGCCACTTGCGTGGAGTTGTTTCGCGAGGAGGTGGTGCCGGTGTGTGCGCCAGAGCTGCTGGCCGGGCGCAAGCTGGCCGGTGCCGAGGAGCTGGCCGAGCTgg                                   <  8:60976/149‑1 (MQ=255)
                                                                                                                    ggCTGACGTGGTGTTCTTCTATGGCCAGGGCACCTGGCCGGGGGCCACTTGCGTGGAGTTGTTTCGCGAGGAGGTGGTGCCGGTGTGTGCGCCAGAGCTGCTGGCCGGGCGCAAGCTGGCCGGTGCCGAGGAGCTGGCCGAGCTGGtgc                                >  6:71698/1‑149 (MQ=255)
                                                                                                                           gtggtgTTCTTCTATGGCCAGGGCACCTGGCCGGGGGCCACTTGCGTGGAGTTGTTTCGCGAGGAGGTGGTGCCGGTGTGTGCGCCAGAGCTGCTGGCCGGGCGCAAGCTGGCCGGTGCCGAGGAGCTGGCCGAGCTGGtgctgctgc                          >  2:198671/1‑148 (MQ=255)
                                                                                                                             ggtgTTCTTCTATGGCCAGGGCACCTGGCCGGGGGCCACTTGCGTGGTGTTGTTTCGCGAGGAGGTGGTGCCGGTGTGTGCGCCAGAGCTGCTGGCCGGGCGCAAGCTGGCCGGTGCCGAGGAGCTGGCCGAGCTGGTGCTGCTGCa                         >  2:93836/1‑147 (MQ=255)
                                                                                                                               tgtTCTTCTATGGCCAGGGCACCTGGCCGGGGGCCACTTGCGTGGAGTTGTTTCGCGAGGAGGTGGTGCCGGTGTGTGCGCCAGAGCTGCTGGCCGGGCGCAAGCTGGCCGGTGCCGAGGAGCTGGCCGAGCTGGTGCTGCTGCAAAGc                     <  3:228165/149‑1 (MQ=255)
                                                                                                                                 ttcttcTATGGCCAGGGCACCTGGCCGGGGGCCACTTGCGTGGAGTTGTTTCGCGAGGAGGTGGTGCCGGTGTGTGCGCCAGAGCTGCTGGCCGGGCGCAAGCTGGCCGGTGCCGAGGAGCTGGCCGAGCTGGTGCTGCTGCAAAGCAc                   >  3:263741/1‑149 (MQ=255)
                                                                                                                                 ttcttcTATGGCCAGGGCACCTGGCCGGGGGCCACTTGCGTGGAGTTGTTTCGCGAGGAGGTGGTGCCGGTGTGTGCGCCAGAGCTGCTGGCCGGGCGCAAGCTGGCCGGTGCCGAGGAGCTGGCCGAGCTGGTGCTGCTGCAAAGCAc                   <  1:223221/149‑1 (MQ=255)
                                                                                                                                 ttcttcTATGGCCAGGGCACCTGGCCGGGGGCCACTTGCGTGGAGTTGTTTCGCGAGGAGGTGGTGCCGGTGTGTGCGCCAGAGCTGCTGGCCGGGCGCAAGCTGGCCGGTGCCGAGGAGCTGGCCGAGCTGGTGCTGCTGCAAAGCAc                   <  6:177210/149‑1 (MQ=255)
                                                                                                                                      ctATGGCCAGGGCACCTGGCCGGGGGCCACTTGCGTGGAGTTGTTTCGCGAGGAGGTGGTGCCGGTGTGTGCGCCAGAGCTGCTGGCCGGGCGCAAGCAGGCCGGTGCCGAGGAGCTGGCCGAGCTGGTGCTGCTGCAAAGCACCTcgc              >  2:142962/1‑149 (MQ=255)
                                                                                                                                      ccccggCCAGGTGCCCTGGCCAGGGGCCACTTGCGTGGAGTTGTTTCGCGAGGAGGTGGTGCCGGTGTGTGCGCCAGAGCTGCTGGCCGGGCGCAAGCTGGCCGGTGCCGAGGAGCTGGCCGCGCTGGTGCTGCTGCAAAGCACCTcgc              >  7:176372/5‑149 (MQ=255)
                                                                                                                                             cAGGGCACCTGGCCGGGGGCCACTTGCGTGGAGTTGTTTCGCGAGGAGGTGGTGCCGGTGTGTGCGCCAGAGCTGCTGGCCGGGCGCAAGCTGGCCGGTGCCGAGGAGCTGGCCGAGCTGGTGCTGCTGCAAAGCACCTCGCGCCCGGa       <  7:82235/149‑1 (MQ=255)
                                                                                                                                             cAGGGCACCTGGCCGGGGGCCACTTGCGTGGAGTTGTTTCGCGAGGAGGTGGTGCCGGTGTGTGCGCCAGAGCTGCTGGCCGGGCGCAAGCTGGCCGGTGCCGAGGAGCTGGCCGAGCTGGTGCTGCTGCAAAGCACCTCGCGCCCGGa       <  4:115501/149‑1 (MQ=255)
                                                                                                                                             cAGGGCACCTGGCCGGGGGCCACTTGCGTGGAGTTGTTTCGCGAGGAGGTGGTGCCGGTGTGGGCGCCAGAGCTGCTGGCCGGGCGCAAGCTGGCCGGTGCCGAGGAGCTGGCCGAGCTg                                    <  2:246456/120‑1 (MQ=255)
                                                                                                                                             cAGGGCACCTGGCCGGGGGCCACTTGCGTGGAGTTGTTTCGCGAGCAGGTGGTGCCGGTGTGTGCGCCAGAGCTGCTGGCCGGGCGCAAGCTGGCCGGTGCCGAGGAGCTGGCCGAGCTg                                    >  1:246456/1‑120 (MQ=255)
                                                                                                                                                ggCACCTGGCCGGGGGCCACTTGCGTGGAGTTGTTTCGCGAGGAGGTGGTGCCGGTGTGTGCGCCAGAGCTGCTGGCCGGGCGCAAGCTGGCCGGTGCCGAGGAGCTGGCCGAGCTGGTGCTGCTGCAAAGCACCTCGCGCCCGGAGGc    >  6:77892/1‑149 (MQ=255)
                                                                                                                                                  cACCTGGCCGGGGGCCACTTGCGTGGAGTTGTTTCGCGAGGAGGTGGTGCCGGTGTGTGCGCCAGAGCTGCTGGCCGGGCGCAAGCTGGCCGGTGCCGAGGAGCTGGCCGAGCTGGTGCTGCTGCAAAGCACCTCGCGCCCGGAGGCCt  >  7:295193/1‑149 (MQ=255)
                                                                                                                                                   |                                                                                                                                                   
CCGAGTTTTGGCGTGCGCTGGTTGATCCCGCACCTCAAGGGGTTTGGCAAACGGCACGGCAATATCCACCTGGACATTCGCAACGAGATGGAGCCGTTCGCCCTGCTGCAAGGCTCGGCTGACGTGGTGTTCTTCTATGGCCAGGGCACCTGGCCGGGGGCCACTTGCGTGGAGTTGTTTCGCGAGGAGGTGGTGCCGGTGTGTGCGCCAGAGCTGCTGGCCGGGCGCAAGCTGGCCGGTGCCGAGGAGCTGGCCGAGCTGGTGCTGCTGCAAAGCACCTCGCGCCCGGAGGCCT  >  NC_002947/6004381‑6004675

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑