Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 6,023,160 T→C 12.0% intergenic (+75/+69) PP_5273 → / ← PP_5274 oxidoreductase/hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029476,023,1600TC12.0% 123.4 / 7.2 50intergenic (+75/+69)PP_5273/PP_5274oxidoreductase/hypothetical protein
Reads supporting (aligned to +/- strand):  ref base T (21/23);  new base C (3/3);  total (24/26)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CTGGCGGCAAGCACCTGCGCTCGCCACTGCTGGCGCTGGGAATGCTGTGGCATCGCCTCAAAGAGCTGACTTGAAAGTCCCCATCCTTGTGGCAGCAACTGTCTTGCTTAAAATCTAAAAGCTGACGCGCCCCCTGTGGGAGCGGGCGTGCCCGCTCCCACAGGGTCGGTGCAAGTTTCAAGTCTGGGGAACAACAGGCCCTCCAGCGACCATCGTGTCAGTCCTTCCAGAAGGGTTTCCGCCCTTCGATCCGGGCCTGCTCCCAGCTCAGCCCGACATCGCGCAACTCGTCATCGG  >  NC_002947/6023012‑6023308
                                                                                                                                                    |                                                                                                                                                    
cTGGCGGCAAGCACCTGCGCTCGCCACTGCTGGCGCTGGGAATGCTGTGGCATCGCCTCAAAGAGCTGACTTGAAAGTCCCCATCCTTGTGGCAGCAACTGTCTTGCTTAAAATCTAAAAGCTGACGCGCCCCCTGTGGGAGCGGGCGt                                                                                                                                                      <  2:6995/149‑1 (MQ=255)
  tgcggcAAGCACCTGCGCTCGCCACTGCTGGCGCTGGGAATGCTGTGGCATCGCCTCAAAGAGCTGACTTGAAAGTCCCCATCCTTGTGGCAGCAACTGTCTTGCTTAAAATCTAAAAGCTGACGCGCCCCCTGTGGGAGCGGGCGTg                                                                                                                                                     <  8:240060/147‑1 (MQ=255)
    cggcAAGCACCTGCGCTCGCCACTGCTGGCGCTGGGAATGCTGTGGCATCGCCTCAAAGAGCTGACTTGAAAGTCCCCATCCTTGTGGCAGCAACTGTCTTGCTTAAAATCTAAAAGCTGACGCGCCCCCTGTGGGAGCGGGCGTGccc                                                                                                                                                  <  2:79900/149‑1 (MQ=255)
        aaGCACCTGCGCTCGCCACTGCTGGCGCTGGGAATGCTGTGGCATCGCCTCAAAGAGCTGACTTGAAAGTCCCCATCCTTGTGGCAGCAACTGTCTTGCTTAAAATCTAAAAGCTGACGCGCCCCCTGTGGGAGCGGGCGTGCCCGCTc                                                                                                                                              <  7:70736/149‑1 (MQ=255)
         aGCACCTGCGCTCGCCACTGCTGGCGCTGGGAATGCTGTGGCATCGCCTCAAAGAGCTGACTTGAAAGTCCCCATCCTTGTGGCAGCAACTGTCTTGCTTAAAATCTAAAAGCTGACGCGCCCCCTGTGGGAGCGGGCGTGCCCGCTcc                                                                                                                                             >  5:255291/1‑149 (MQ=255)
            aCCTGCGCTCGCCACTGCTGGCGCTGGGAATGCTGTGGCATCGCCTCAAAGAGCTGACTTGAAAGTCCCCATCCTTGTGGCAGCAACTGTCTTGCTTAAAATCTAAAAGCTGACGCGCCCCCTGTGAGAGCGGGCACGCCCGCTCCcac                                                                                                                                          <  5:13399/149‑1 (MQ=255)
                    tCGCCACTGCTGGCGCTGGGAATGCTGTGGCATCGCCTCAAAGAGCTGACTTGAAAGTCCCCATCCTTGTGGCAGCAACTGTCTTGCTTAAAATCTAAAAGCTGACGCGCCCCCTGTGGGAGCGGGCGTGcc                                                                                                                                                   <  2:159935/132‑1 (MQ=255)
                       ccACTGCTGGCGCTGGGAATGCTGTGGCATCGCCTCAAAGAGCTGACTTGAAAGTCCCCATCCTTGTGGCAGCAACTGTCTTGCTTAAAATCTAAAAGCTGACGCGCCCCCTGTGGGAGCGGGCGTGCCCGCTCCCACAGGGTCGGTg                                                                                                                                >  8:67513/1‑148 (MQ=255)
                             ctgGCGCTGGGAATGCTGTGGCATCGCCTCAAAGAGCTGACTTGAAAGTCCCCATCCTTGTGGCAGCAACTGTCTTGCTTAAAATCTAAAAGCTGACGCGCCCCCTGTGGGAGCGGGCGTGCCCGCTc                                                                                                                                              >  5:311687/1‑128 (MQ=255)
                             ctgGCGCTGGGAATGCTGTGGCATCGCCTCAAAGAGCTGACTTGAAAGTCCCCATCCTTGTGGCAGCAACTGTCTTGCTTAAAATCTAAAAGCTGACGCGCCCCCTGTGGGAGCGGGCGTGCCCGCTc                                                                                                                                              <  6:311687/128‑1 (MQ=255)
                                gcgcTGGGAATGCTGTGGCATCGCCTCAAAGAGCTGACTTGAAAGTCCCCATCCTTGTGGCAGCAACTGTCTTGCTTAAAATCTAAAAGCTGACGCGCCCCCTGTGGGAGCGGGCGTGCCCGCTCCCACAGGGTCGGTGCAAGTTTc                                                                                                                        <  6:255291/147‑1 (MQ=255)
                                    tGGGAATGCTGTGGCATCGCCTCAAAGAGCTGACTTGAAAGTCCCCATCCTTGTGGCAGCAACTGTCTTGCTTAAAATCTAAAAGCTGACGCGCCCCCTGTGGGAGCGGGCGTGCCCGCTCCCACAGGGTCGGTGCAAGTTTCAAGTCt                                                                                                                  >  7:289508/1‑149 (MQ=255)
                                    tGGGAATGCTGTGGCATCGCCTCAAAGAGCTGACTTGAAAGTCCCCATCCTTGTGGCAGCAACTGTCTTGCTTAAAATCTAAAAGCTGACGCGCCCCCTGTGGGAGCGGGCGTGCCCGCTCCCACAGGGTCGGTGCAAGTTTCAAGTCt                                                                                                                  >  2:124436/1‑149 (MQ=255)
                                     gggAATGCTGTGGCATCGCCTCAAAGACCTTACTTGAAAGTCACCATCCTTGTGGCAGCAACTGTCTTGCTTAAAAACTAAAAGATGACGCGCCCCCTGTGGGAGCGGGCACGCACGCTc                                                                                                                                              >  1:34051/1‑120 (MQ=255)
                                      ggAATGCTGTGGCATCGCCTCAAAGAGCTGACTTGAAAGTCCCCATCCTTGTGGCAGCAACTGTCTTGCTTAAAATCTAAAAGCTGACGCGCCCCCTGTGGGAGCGGGCGTGCCCGCTCCCACAGGGTCGGTGCAAGTTTCAAGTCTgg                                                                                                                <  7:196067/149‑1 (MQ=255)
                                                 gCATCGCCTCAAAGAGCTGACTTGAAAGTCCCCATCCTTGTGGCAGCAACTGTCTTGCTTAAAATCTAAAAGCTGACGCGCCCCCTGTGGGAGCGGGCGTGCCCGCTCCCACAGGGTCGGTGCAAGTTTCAAGTCTGGGGAACAACAgg                                                                                                     >  5:292413/1‑149 (MQ=255)
                                                        cTCAAAGAGCTGACTTGAAAGTCCCCATCCTTGTGGCAGCAACTGTCTTGCTTAAAATCTAAAAGCTGACGCGCCCCCTGTGGGAGCGGGCGTGCCCGCTCCCACAGGGTCGGTGCAAGTTTCAAGTCTGGGGAACAACAGGCCCTCCa                                                                                              <  7:67513/149‑1 (MQ=255)
                                                              gagCTGACTTGAAAGTCCCCATCCTTGTGGCAGCAACTGTCTTGCTTAAAATCTAAAAGCTGCCGCGCCCCCTGTGGGAGCGGGCGTGCCCGCTCCCACAGGGTCGGTGCAAGTTTCAAGTCTGGGGAACAACAGGCCCTCCAGCGAcc                                                                                        <  7:128851/149‑1 (MQ=255)
                                                                   gACTTGAAAGTCCCCATCCTTGTGGCAGCAACTGTCTTGCTTAAAATCTAAAAGCTGACGCGCCCCCTGTGGGAGCGGGCGTGCCCGCTCCCACAGGGTCGGTGCAAGTTTCAAGTCTGGGGAACAACAGGCCCTCCAGCGACCATCgt                                                                                   >  8:235371/1‑149 (MQ=255)
                                                                     cTTGAAAGTCCCCATCCTTGTGGCAGCAACTGTCTTGCTTAAAATCTAAAAGCTGACGCGCCCCCTGTGGGAGCGGGCGTGCCCGCTCCCACAGGGTCGGTGCAAGTTTCAAGTCTGGGGAACAACAGGCCCTCCAGCGACCATCgtg                                                                                  <  7:235371/148‑1 (MQ=255)
                                                                      ttGAAAGTCCCCATCCTTGTGGCAGCAACTGTCTTGCTTAAAATCTAAAAGCTGACGCGCCCCCTGTGGGAGCGGGCGTGCCGGCTCCCACAGGGTCGGTGCAAGTTTCAAGTCTGGGGAACAACAGGCCCTCCAGCGACCATCGTGTc                                                                                <  8:160860/149‑1 (MQ=255)
                                                                      ttGAAAGTCCCCATCCTTGTGGCAGCAACTGTCTTGCTTAAAATCTAAAAGCTGACGCGCCCCCTGTGGGAGCGGGCGTGCCCGCTCCCACAGGGTCGGTGCAAGTTTCAAGTCTggg                                                                                                               >  6:235272/1‑118 (MQ=255)
                                                                      ttGAAAGTCCCCATCCTTGTGGCAGCAACTGTCTTGCTTAAAATCTAAAAGCTGACGCGCCCCCTGTGGGAGCGGGCGTGCCCGCTCCCACAGGGTCGGTGCAAGTTTCAAGTCTggg                                                                                                               <  5:235272/118‑1 (MQ=255)
                                                                      ttGAAAGTCCCCATCCTTGTGGCAGCAACTGTCTTGCTTAAAATCTAAAAGCTGACGCGCCCCCTGTGGGAGCGGGCGTGCCCGCTCCCACAGGGTCGGTGCAAGTTTCAAGTCTgg                                                                                                                >  3:42202/1‑117 (MQ=255)
                                                                      ttGAAAGTCCCCATCCTTGTGGCAGCAACTGTCTTGCTTAAAATCTAAAAGCTGACGCGCCCCCTGTGGGAGCGGGCGTGCCCGCTCCCACAGGGTCGGTGCAAGTTTCAAGTCTgg                                                                                                                <  4:42202/117‑1 (MQ=255)
                                                                               cccATCCTTGTGGCAGCAACTGTCTTGCTTAAAATCTAAAAGCTGACGCGCCCCCTGTGGGAGCGGGCGTGCCCGCTCCCACAGGGTCGGTGCAAGTTTCAAGTCTGGGGAACAACAGGCCCTCCAGCGACCATCgt                                                                                   >  8:74170/1‑137 (MQ=255)
                                                                               cccATCCTTGTGGCAGCAACTGTCTTGCTTAAAATCTAAAAGCTGACGCGCCCCCTGTGGGAGCGGGCGTGCCCGCTCCCACAGGGTCGGTGCAAGTTTCAAGTCTGGGGAACAACAGGCCCTCCAGCGACCATCgt                                                                                   <  7:74170/137‑1 (MQ=255)
                                                                               cccATCCTTGTGGCAGCAACTGTCTTGCTTAAAATCTAAAAGCTGACGCGCCCCCTGTGGGAGCGGGCGTGCCCGCTCCCACAGGGTCGGTGCAAGTTTCAAGTCTGGGGAACAACAGGCCCTCCAGCGACCATCGTGTCAGTCCTTc                                                                        <  8:289508/148‑1 (MQ=255)
                                                                                ccATCCTTGTGGCAGCAACTGTCTTGCTTAAAATCTAAAAGCTGACGCGCCCCCTGTGGGAGCGGGCGTGCCCGCTCCCACAGGGTCGGCGCAAGTTTCAAGTCTGGGGAACAACAGGCCCTCCAGCGACCATCGTGTCAGTCCTTCCa                                                                      >  4:72688/1‑149 (MQ=255)
                                                                                 cATCCTTGTGGCAGCAACTGTCTTGCTTAAAATCTAAAAGCTGACGCGCCCCCTGTGGGAGCGGGCACGCCCGCt                                                                                                                                               <  3:84150/75‑1 (MQ=255)
                                                                                 cATCCTTGTGGCAGCAACTGTCTTGCTTAAAATCTAAAAGCTGACGCGCCCCCTGTGGGAGCGGGCACGCCCGCt                                                                                                                                               >  4:84150/1‑75 (MQ=255)
                                                                                   tCCTTGTGGCAGCAACTGTCTTGCTTAAAATCTAAAAGCTGACGCGCCCCCTGTGGGAGCGGGCGTGCCCGCTCCCACAGGGTCGGTGCAAGTTTCAAGTCTGGGGAACAACAGGCCCTCCAGCGACCATCGTGTCAGTCCTTCCAGaa                                                                   <  6:292413/149‑1 (MQ=255)
                                                                                                aaCTGTCTTGCTTAAAATCTAAAAGCTGACGCGCCCCCTGTGGGAGCGGGCGTGCCCGCTCCCACAGGGTCGGTGCAAGTTTCAAGTCTGGGGAACAACAGGCCCTCCAGCGACCATCGTGTCAGTCCTTCCAGAAGGGTTTCCGCCCt                                                      <  1:124436/149‑1 (MQ=255)
                                                                                                aaCTGTCTTGCTTAAAATCTAAAAGCTGACGCGCCCCCTGTGGGAGCGGGCGTGCCCGCTCCCACAGGGTCGGTGCAAGTTTCAAGTCTGGGGAACAACAGGCCCTCCAGCGACCATCGTGTCAGTCCTTCCAGAAGGGTTTCCGCCCt                                                      >  1:232027/1‑149 (MQ=255)
                                                                                                     tCTTGCTTAAAATCTAAAAGCTGACGCGCCCCCTGTGGGAGCGGGCGTGCCCGCTCCCACAGGGTCGGTGCAAGTTTCAAGTCTGGGGAACAACAGGCCCTCCAGCGACCATCGTGTCa                                                                               >  7:41182/1‑119 (MQ=255)
                                                                                                     tCTTGCTTAAAATCTAAAAGCTGACGCGCCCCCTGTGGGAGCGGGCGTGCCCGCTCCCACAGGGTCGGTGCAAGTTTCAAGTCTGGGGAACAACAGGCCCTCCAGCGACCATCGTGTCa                                                                               <  8:41182/119‑1 (MQ=255)
                                                                                                           ttAAAATCTAAAAGCTGACGCGCCCCCTGTGGGAGCGGGCGTGCCCGCTCCCACAGGGTCGGTGCAAGTTTCAAGTCTGGGGAACAACAGGCCCTCCAGCGACCATCGTGTCAGTCCTTCCAGAAGGGTTTCCGCCCTTCGATCCGGGc                                           >  2:49321/1‑149 (MQ=255)
                                                                                                                aTCTAAAAGCTGACGCGCCCCCTGTGGGAGCGGGCGTGCCCGCTCCCACAGGGTCGGTGCAAGTTTCAAGTCTGGGGAACAACAGGCCCTCCAGCGACCATCGTGTCAGTCCTTCCAGAAGGGTTTCCGCCCTTCGATCCGGGCCTGCt                                      >  3:274550/1‑149 (MQ=255)
                                                                                                                 tCTAAAAGCTGACGCGCCCCCTGTGGGAGCGGGCGTGCCCGCTCCCACAGGGTCGGTGCAAGTTTCAAGTCTGGGGAACAACAGGCCCTCCAGCGACCATCgt                                                                                   <  7:327533/103‑1 (MQ=255)
                                                                                                                 tCTAAAAGCTGACGCGCCCCCTGTGGGAGCGGGCGTGCCCGCTCCCACAGGGTCGGTGCAAGTTTCAAGTCTGGGGAACAACAGGCCCTCCAGCGACCATCgt                                                                                   >  8:327533/1‑103 (MQ=255)
                                                                                                                     aaaGCTGACGCGCCCCCTGTGGGAGCGGGCGTGCCCGCTCCCACAGGGTCGGTGCAAGTTTCAAGTCTGGGGAACAACAGGCCCTCCAGCGACCATCGTGTCAGTCCTTCCAGAAGGGTTTCCGCCCTTCGATCCGGGCCTGCTCCCAg                                 <  2:232027/149‑1 (MQ=255)
                                                                                                                      aaGCTGACGCGCCCCCTGTGGGAGCGGGCGTGCCCGCTCCCACAGGGTCGGTGCAAGTTTCAAGTCTGGGGAACAACAGGCCCTCCAGCGACCATCGTGTCAGTCCTTCCAGAAGGGTTTCCGCCCTTCGATCCGGGCCTGCTCCCAGc                                >  1:65755/1‑149 (MQ=255)
                                                                                                                            aCGCGCCCCCTGTGGGAGCGGGCGTGCCCGCTCCCACAGGGTCGGTGCAAGTTTCAAGTCTGGGGAACAACAGGCCCTCCAGCGACCATCGTGTCAGTCCTTCCAGAAGGGTTTCCGCCCTTCGATCCGGGCCTGCTcc                                    <  7:282148/139‑1 (MQ=255)
                                                                                                                            aCGCGCCCCCTGTGGGAGCGGGCGTGCCCGCTCCCACAGGGTCGGTGCAAGTTTCAAGTCTGGGGAACAACAGGCCCTCCAGCGACCATCGTGTCAGTCCTTCCAGAAGGGTTTCCGCCCTTCGATCCGGGCCTGCTcc                                    >  8:282148/1‑139 (MQ=255)
                                                                                                                                 cccccTGTGGGAGCGGGCGTGCCCGCTCCCACAGGGTCGGTGCAAGTTTCAAGTCTGGGGAACAACAGGCCCTCCAGCGACCATCGTGTCAGTCCTTCCAGAAGGGTTTCCGCCCTTCGATCCGGGCCTGCTCCCAGCTCAGCCCGACa                     <  1:49321/149‑1 (MQ=255)
                                                                                                                                    ccTGTGGGAGCGGGCACGCCCGCTCCCACAGGGTCGGTGCAAGTTTCAAGTCTGGGGAACAACAGGCCCTCCAGCGACCATCGTGTCAGTCCTTCCAGAAGGGTTTCCGCCCTTCGATCCGGGCCTGCTCCCa                                  <  3:80767/133‑1 (MQ=255)
                                                                                                                                    ccTGTGGGAGCGGGCACGCCCGCTCCCACAGGGTCGGTGCAAGTTTCAAGTCTGGGGAACAACAGGCCCTCCAGCGACCATCGTGTCAGTCCTTCCAGAAGGGTTTCCGCCCTTCGATCCGGGCCTGCTCCCa                                  >  4:80767/1‑133 (MQ=255)
                                                                                                                                      tgtgGGAGCGGGCGTGCCCGCTCCCACAGGGTCGGTGCAAGTTTCAAGTCTGGGGAACAACAGGCCCTCCAGCGACCATCGTGTCAGTCCTTCCAGAAGGGTTTCCGCCCTTCGATCCGGGCCTGCTCCCAGCTCAGCCCGACATcgcg                >  4:246730/1‑149 (MQ=255)
                                                                                                                                          ggAGCGGGCGTGCCCGCTCCCACAGGGTCGGTGCAAGTTTCAAGTCTGGGGAACAACAGGCCCTCCAGCGACCATCGTGTCAGTCCTTCCAGAAGGGTTTCCGCCCTTCGATCCGGGCCTGCTCCCAGCTCAGCCCGACATCGCGCAAc            >  2:19642/1‑149 (MQ=255)
                                                                                                                                                 gCGTGCCCGCTCCCACAGGGTCGGTGCAAGTTTCAAGTCTGGGGAACAACAGGCCCTCCAGCGACCATCGTGTCAGTCCTTCCAGAAGGGTTTCCGCCCTTCGATCCGGGCCTGCTCCCAGCTCAGCCCGACATCGCGCAACTCGTCAt     <  1:19642/149‑1 (MQ=255)
                                                                                                                                                 gCGTGCCCGCTCCCACAGGGTCGGTGCAAGTTTCAAGTCTGGGGAACAACAGGCCCTCCAGCGACCATCGTGTCAGTCCTTCCAGAAGGGTTTCCGCCCTTCGATCCGGGCCTGCTCCCAGCTCAGCCCGACATCGCGCAACTCGTCAt     >  5:96953/1‑149 (MQ=255)
                                                                                                                                                    tGCCCGCTCCCACAGGGTCGGTGCAAGTTTCAAGTCTGGGGAACAACAGGCCCTCCAGCGACCATCGTGTCAGTCCTTCCAGAAGGGTTTCCGCCCTTCGATCCGGGCCTGCTCCCAGCTCAGCCCGACATCGCGCAACTCGTCATCgg  <  3:246730/149‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                    
CTGGCGGCAAGCACCTGCGCTCGCCACTGCTGGCGCTGGGAATGCTGTGGCATCGCCTCAAAGAGCTGACTTGAAAGTCCCCATCCTTGTGGCAGCAACTGTCTTGCTTAAAATCTAAAAGCTGACGCGCCCCCTGTGGGAGCGGGCGTGCCCGCTCCCACAGGGTCGGTGCAAGTTTCAAGTCTGGGGAACAACAGGCCCTCCAGCGACCATCGTGTCAGTCCTTCCAGAAGGGTTTCCGCCCTTCGATCCGGGCCTGCTCCCAGCTCAGCCCGACATCGCGCAACTCGTCATCGG  >  NC_002947/6023012‑6023308

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑