Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 6,026,544 G→T 7.8% V472V (GTG→GTT)  PP_5276 → phospholipase D family protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029476,026,5440GT 7.8% 145.3 / 2.0 47V472V (GTG→GTT) PP_5276phospholipase D family protein
Reads supporting (aligned to +/- strand):  ref base G (21/22);  new base T (2/2);  total (23/24)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.94e-01

TTCATCGGCTCGTTCAACTTCGACCCGCGCTCAGTATTGTGGAACACCGAGGTCGGGGTACTGGTGGACAGCCCGGAGCTGGCCGAATACACCCGCGAACTGGCCCAGCAGGGTATGGCGCCTGCGCTGAGCTATCAGGTGAAACTGATAGGTAACAAGCTGGTGTGGGCGACCGAGGACAATGGCCAACGGCACGTGCTGACGTCTGAGCCGGGCGGGATCTGGCGACGCTTCAATGCCTGGATCAGCAAGGCGGTTGGCTTGGAGAAGATGCTATAGCGGC  >  NC_002947/6026404‑6026686
                                                                                                                                            |                                                                                                                                              
ttCATCGGCTCGTTCAACTTCGACCCGCGCTCAGTATTGTGGAACACCGAGGTCGGGGTACTGGTGGACAGCCCGGAGCTGGCCGAATACACCCGCGAACTGGCCCAGCAGGGTATGGCGCCTGCGCTGAGCTATCAGGTGAAAc                                                                                                                                            >  8:130768/1‑145 (MQ=255)
ttCATCGGCTCGTTCAACTTCGACCCGCGCTCAGTATTGTGGAACACCGAGGTCGGGGTACTGGTGGACAGCCCGGAGCTGGCCGAATACACCCGCGAACTGGCCCAGCAGGGTATGGCGCCTGCGCTGAGCTATCAGGTGAAAc                                                                                                                                            <  7:130768/145‑1 (MQ=255)
   aTCGGCTCGTTCAACTTCGACCCGCGCTCAGTATTGTGGAACACCGAGGTCGGGGTACTGGTGGACAGCCCGGAGCTGGCCGAATACACCCGCGAACTGGCCCAGCAGGGTATGGCGCCTGCGCTGAGCTATCAGGTGAAACTGATAgg                                                                                                                                     >  3:342676/1‑149 (MQ=255)
    tCGGCTCGTTCAACTTCGACCCGCGCTCAGTATTGTGGAACACCGAGGTCGGGGTACTGGTGGACAGCCCGGAGCTGGCCGAATACACCCGCGAACTGGCCCAGCAGGGTATGGCGCCTGCGCTGAGCTATCAGTTTCACCTGATAg                                                                                                                                      <  4:23814/147‑1 (MQ=255)
    tCGGCTCGTTCAACTTCGACCCGCGCTCAGTATTGTGGAACACCGAGGTCGGGGTACTGGTGGACAGCCCGGAGCTGGCCGAATACACCCGCGAACTGGCCCAGCAGGGTATGGCGCCTGCGCTGAGCTATCAGTTTCACCTGATAg                                                                                                                                      >  3:23814/1‑147 (MQ=255)
      ggCTCGTTCAACTTCGACCCGCGCTCAGTATTGTGGAACACCGAGGTCGGGGTACTGGTGGACAGCCCGGAGCTGGCCGAATACACCCGCGAACTGGCCCAGCAGGGTATGGCGCCTGCGCTGAGCTATCAGGTGAAACTGATAGGTaa                                                                                                                                  >  8:23201/1‑149 (MQ=255)
         tCGTTCAACTTCGACCCGCGCTCAGTATTGTGGAACACCGAGGTCGGGGTACTGGTGGACAGCCCGGAGCTGGCCGAATACACCCGCGAACTGGCCCAGCAGGGTATGGCGCCTGCGCTGAGCTATCAGGTGAAAc                                                                                                                                            >  2:93960/1‑136 (MQ=255)
         tCGTTCAACTTCGACCCGCGCTCAGTATTGTGGAACACCGAGGTCGGGGTACTGGTGGACAGCCCGGAGCTGGCCGAATACACCCGCGAACTGGCCCAGCAGGGTATGGCGCCTGCGCTGAGCTATCAGGTGAAAc                                                                                                                                            <  1:93960/136‑1 (MQ=255)
           gTTCAACTTCGACCCGCGCTCAGTATTGTGGAACACCGAGGTCGGGGTACTGGTGGACAGCCCGGAGCTGGCCGAATACACCCGCGAACTGGCCCAGCAGGGTATGGCGCCTGCGCTGAGCTATCAGGTGAAACTGATAg                                                                                                                                      <  2:59798/140‑1 (MQ=255)
           gTTCAACTTCGACCCGCGCTCAGTATTGTGGAACACCGAGGTCGGGGTACTGGTGGACAGCCCGGAGCTGGCCGAATACACCCGCGAACTGGCCCAGCAGGGTATGGCGCCTGCGCTGAGCTATCAGGTGAAACTGATAg                                                                                                                                      >  1:59798/1‑140 (MQ=255)
           gTTCAACTTCGACCCGCGCTCAGTATTGTGGAACACCGAGGTCGGGGTACTGGTGGACAGCCCGGAGCTGGCCGAATACACCCGCGAACTGGCCCAGCAGGGTATGGCGCCTGCGCTGAGCTATCAGGTGAAACTGATAGGTAACaa                                                                                                                               <  3:309909/147‑1 (MQ=255)
              cAACTTCGACCCGCGCTCAGTATTGTGGAACACCGAGGTCGGGGTACTGGTGGACAGCCCGGAGCTGGCCGAATACACCCGCGAACTGGCCCAGCAGGGTATGGCGCCTGCGCTGAGCTATCAGGTGAAACTGATAGGTAACAAGCTg                                                                                                                           <  4:342676/148‑1 (MQ=255)
                       cccGCGCTCAGTATTGTGGAACACCGAGGTCGGGGTACTGGTGGACAGCCCGGAGCTGGCCGAATACACCCGCGAACTGGCCCAGCAGGGTATGGCGCCTGCGCTGAGCTATCAGGTGAAACTg                                                                                                                                          >  7:53382/1‑124 (MQ=255)
                       cccGCGCTCAGTATTGTGGAACACCGAGGTCGGGGTACTGGTGGACAGCCCGGAGCTGGCCGAATACACCCGCGAACTGGCCCAGCAGGGTATGGCGCCTGCGCTGAGCTATCAGGTGAAACTg                                                                                                                                          <  8:53382/124‑1 (MQ=255)
                           cgcTCAGTATTGTGGAACACCGAGGGCGGGGTACTGGTGGACAGCCCGGAGCTGGCCGAATACACCCGCGAACTGGCCCAGCAGGGTATGGCGCCTGCGCTGAGCTATCAGGTGAAACTGATAGGTAACAAGCTGGTGTGGGCGACCGa                                                                                                             <  2:122891/149‑1 (MQ=255)
                                       tgGAACACCGAGGTCGGGGTACTGGTGGACAGCCCGGAGCTGGCCGAATACACCCGCGAACTGGCCCAGCAGGGTATGGCGCCTGCGCTGAGCTATCAGGTGAAACTGATAGGTAACAAGCTGGTGTGGGCGACCGAg                                                                                                            >  3:231784/1‑138 (MQ=255)
                                       tgGAACACCGAGGTCGGGGTACTGGTGGACAGCCCGGAGCTGGCCGAATACACCCGCGAACTGGCCCAGCAGGGTATGGCGCCTGCGCTGAGCTATCAGGTGAAACTGATAGGTAACAAGCTGGTGTGGGCGACCGAg                                                                                                            <  4:231784/138‑1 (MQ=255)
                                         gAACACCGAGGTCGGGGTACTGGTGGACAGCCCGGAGCTGGCCGAATACACCCGCGAACTGGCCCAGCAGGGTATGGCGCCTGCGCTGAGCTATCAGGTGAAACTGATAGGTAACAAGCTGGTGTGGGCGACCGAGGACAATGGCCaa                                                                                                >  2:30035/1‑148 (MQ=255)
                                         gAACACCGAGGTCGGGGTACTGGTGGACAGCCCGGAGCTGGCCGAATACACCCGCGAACTGGCCCAGCAGGGTATGGCGCCTGCGCTGAGCTATCAGGTGAAACTGATAGGTAACAACCTGGTGTGGGCGACCGAGGACAATGGCCaa                                                                                                <  1:30035/148‑1 (MQ=255)
                                                            cTGGTGGACAGCCCGGAGCTGGCCGAATACACCCGCGAACTGGCCCAGCAGGGTATGGCGCCTGCGCTGAGCTATCAGGTGAAACTGATAGGTAACAAGCTGGTGTGGGCGACCGAGGACAATGGCCAACGGCACGTGCTGACGTCTGa                                                                            <  5:333119/149‑1 (MQ=255)
                                                               gtggACAGCCCGGAGCTGGCCGAATACACCCGCGAACTGGCCCAGCAGGGTATGGCGCCTGCGCTGAGCTATCAGGTGAAACTGATAGGTAACAAGCTGGTGTGGGCGACCGAGGACAATg                                                                                                     <  5:20943/121‑1 (MQ=255)
                                                               gtggACAGCCCGGAGCTGGCCGAATACACCCGCGAACTGGCCCAGCAGGGTATGGCGCCTGCGCTGAGCTATCAGGTGAAACTGATAGGTAACAAGCTGGTGTGGGCGACCGAGGACAATg                                                                                                     >  6:20943/1‑121 (MQ=255)
                                                               gtggACAGCCCGGAGCTGGCCGAATACACCCGCGAACTGGCCCAGCAGGGTATGGCGCCTGCGCTGAGCTATCAGGTGAAACTGATAGGTAACAAGCTGGTGTGGGCGACCGAGGACAATGGCCAACGGCACGTGCTGACGTCt                                                                              >  1:62667/1‑144 (MQ=255)
                                                               gtggACAGCCCGGAGCTGGCCGAATACACCCGCGAACTGGCCCAGCAGGGTATGGCGCCTGCGCTGAGCTATCAGGTGAAACTGATAGGTAACAAGCTGGTGTGGGCGACCGAGGACAATGGCCAACGGCACGTGCTGACGTCt                                                                              <  2:62667/144‑1 (MQ=255)
                                                                            aGCTGGCCGAATACACCCGCGAACTGGCCCAGCAGGGTATGGCGCCTGCGCTGAGCTATCAGGTGAAACTGATAGGTAACAAGCTGGTGTGGGCGACCGAGGACAATGGCCAACGGCACGTGCTGACGTCTGAGCCGGGCGGGATCTgg                                                            >  7:57579/1‑149 (MQ=255)
                                                                                   cGAATACACCCGCGAACTGGCCCAGCAGGGTATGGCGCCTGCGCTGAGCTATCAGGTGAAACTGATAGGTAACAAGCTGGTGTGGGCGACCGAGGACAATGGCCAACGGCACGTGCTGACGTCTGAGCCGGGCGGGATCTGGCGACGCt                                                     >  1:205797/1‑149 (MQ=255)
                                                                                       tACACCCGCGAACTGGCCCAGCAGGGTATGGCGCCTGCGCTGAGCTATCAGGTGAAACTGATAGGTAACAAGCTGGTGTGGGCGACCGAGGACAATGGCCAACGGCACGTGCTGACGTCTGAGCCGGGCGGGATCTGGCGACGCTTCaa                                                 >  8:155260/1‑149 (MQ=255)
                                                                                         cacCCGCGAACTGGCCCAGCAGGGTATGGCGCCTGCGCTGAGCTATCAGGTGAAACTGATAGGTAACAAGCTGGTGTGGGCGACCGAGGACAATGGCCAACGGCACGTGCTGACGTCTGAGCCGGGCGGGATCTGGCGACGCTTCAATg                                               <  2:205797/149‑1 (MQ=255)
                                                                                            ccGCGAACTGGCCCAGCAGGGTATGGCGCCTGCGCTGAGCTATCAGGTGAAACTGATAGGTAACAAGCTGGTGTGGGCGACCGAGGACAATGGCCAACGGCACGTGCTGACGTCTGAGCCGGGTGGGATCTGGCGACGCTTCAATGCCt                                            >  6:187445/1‑149 (MQ=255)
                                                                                            ccGCGAACTGGCCCAGCAGGGTATGGCGCCTGCGCTGAGCTATCAGGTGAAACTGATAGGTAACAAGCTGGTGTGGGCGACCGAGGACAATGGCCAACGGCACGTGCTGACGTCTGAGCCGGGTGGGATCTGGCGACGCTTCAATGCCt                                            >  6:192404/1‑149 (MQ=255)
                                                                                                 aaCTGGCCCAGCAGGTTATGGCGCCTGCGCTGAGCTATCAGGTGAAACTGATAGGTAACAAGCTGGTGTGGGCGACCGAGGACAATGGCCAACGGCACGTGCTGACGTCTGAGCCGGGCGGGATCTGGCGACGCtt                                                    <  8:123434/136‑1 (MQ=255)
                                                                                                 aaCTGGCCCAGCAGGGTATGGCGCCTGCGCTGAGCTATCAGGTGAAACTGATAGGTAACAAGCTGGTGTGGGCGACCGAGGACAATGGCCAACGGCACGTGCTGACGTCTGAGCCGGGCGGGa                                                                 >  7:274642/1‑123 (MQ=255)
                                                                                                 aaCTGGCCCAGCAGGGTATGGCGCCTGCGCTGAGCTATCAGGTGAAACTGATAGGTAACAAGCTGGTGTGGGCGACCGAGGACAATGGCCAACGGCACGTGCTGACGTCTGAGCCGGGCGGGa                                                                 <  8:274642/123‑1 (MQ=255)
                                                                                                 aaCTGGCCCAGCAGGGTATGGCGCCTGCGCTGAGCTATCAGGTGAAACTGATAGGTAACAAGCTGGTGTGGGCGACCGAGGACAATGGCCAACGGCACGTGCTGACGTCTGAGCCGGGCGGGATCTGGCGACGCtt                                                    >  7:123434/1‑136 (MQ=255)
                                                                                                        ccAGCAGGGTATGGCGCCTGCGCTGAGCTATCAGGTGAAACTGATAGGTAACAAGCTGGTGTGGGCGACCGAGGACAATGGCCAACGGCACGTGCTGACGTCTGAGCCGGGCGGGATCTGGCGACGCTTCAATGCCTGGATCAGCAAgg                                >  8:161379/1‑149 (MQ=255)
                                                                                                        ccAGCAGGGTATGGCGCCTGCGCTGAGCTATCAGGTGAAACTGATAGGTAACAAGCTGGTGTGGGCGACCGAGGACAATGGCCAACGGCACGTGCTGACGTCTGAGCCGGGCGGGATCTGGCGACGCTTCAATGCCTGGATCAGCAAgg                                <  7:155260/149‑1 (MQ=255)
                                                                                                             atggTATGGCGCCTGCGCTGAGCTATCAGGTGAAACTGATAGGTAACAAGCTGGTGTGGGCGACCGAGGACAATGGCCAACGGCACGTGCTGACGTCTGAGCCGGGCGGGATCTGGCGACGCTTCAATGCCTGGATCAGCAAGGCGGtt                           <  8:57579/147‑1 (MQ=255)
                                                                                                                 tATGGCGCCTGCGCTGAGCTATCAGGTGAAACTGATAGGTAACAAGCTGGTGTGGGCGACCGAGGACAATGGCCAACGGCACGTGCTGACGTCTGAGCCGGGCGGGATCTGGCGACGCTTCAATGCCTGGATCAGCAAGGCGGTTGGCt                       <  7:161379/149‑1 (MQ=255)
                                                                                                                     gcgcCTGCGCTGAGCTATCAGGTGAAACTGATAGGTAACAAGCTGGTGTGGGCGACCGAGGACAATGGCCAACGGCACGTGCTGACGTCTGAGCCGGGTGGGATCTGGCGACGCTTCAATGCCTGGATCAGCAAGGCGGTTGGCTTGga                   <  5:192404/149‑1 (MQ=255)
                                                                                                                     gcgcCTGCGCTGAGCTATCAGGTGAAACTGATAGGTAACAAGCTGGTGTGGGCGACCGAGGACAATGGCCAACGGCACGTGCTGACGTCTGAGCCGGGTGGGATCTGGCGACGCTTCAATGCCTGGATCAGCAAGGCGGTTGGCTTGga                   <  5:187445/149‑1 (MQ=255)
                                                                                                                     gcgcCTGCGCTGAGCTATCAGGTGAAACTGATAGGTAACAAGCTGGTGTGGGCGACCGAGGACAATGGCCAACGGCACGTGCTAACGTCTGAGCCGGGCGGGATCTGGCGACGCTTCAATGCCTGGATCAGCAAGGCGGTTGGCTTg                     >  3:89455/1‑147 (MQ=255)
                                                                                                                        ccTGCGCTGAGCTATCAGGTGAAACTGATAGGTAACAAGCTGGTGTGGGCGACCGAGGACAATGGCCAACGGCACGTGCTAACGTCTGAGCCGGGCGGGATCTGGCGACGCTTCAATGCCTGGATCAGCAAGGCGGTTGGCTTGGagaa                <  4:89455/149‑1 (MQ=255)
                                                                                                                               ttaccTATCAGTTTCACCTGATAGGTAACAAGCTGGTGTGGGCGACCGAGGACAATGGCCAACGGCACGTGCTGACGTCTGAGCCGGGCGGGATCTGGCGACGCTTCAATGCCTGGATCAGCAAGGCGGTTGGCTTGGAGAAGATGCta         >  1:249700/5‑149 (MQ=255)
                                                                                                                               tGAGCTATCAGGTGAAACTGATAGGTAACAAGCTGGTGTGGGCGACCGAGGACAATGGCCAACGGCACGTGCTGACGTCTGAGCCGGGCGGGATCTGGCGACGCTTCAATGCCTGGATCAGCAAGGCGGt                            <  4:94634/130‑1 (MQ=255)
                                                                                                                               tGAGCTATCAGGTGAAACTGATAGGTAACAAGCTGGTGTGGGCGACCGAGGACAATGGCCAACGGCACGTGCTGACGTCTGAGCCGGGCGGGATCTGGCGACGCTTCAATGCCTGGATCAGCAAGGCGGt                            >  3:94634/1‑130 (MQ=255)
                                                                                                                                         ggTTAAACTGATAGCTAACAAGCGGGTTTGGGCGACCGAGGACAATGGCCAACGGCACGTGCTGACGTCTGAGCCGGGCGGGATCTGGCGACGCTTCAATGCCTGGATCAGCAAGGCGGTTGGCTTGGAGAAGATGc           <  2:50783/137‑1 (MQ=255)
                                                                                                                                         ggTGAAACTGATAGGTAACAAGCTGGTGTGCGCGACCGAGGACAATGGCCAACGGCACGTGCTGACGTCTGAGCCGGGCGGGATCTGGCGACGCTTCAATGCCTGGATCAGCAAGGCGGTTGGCTTGGAGAAGATGCTAGAtcgga  >  1:50783/1‑145 (MQ=255)
                                                                                                                                            |                                                                                                                                              
TTCATCGGCTCGTTCAACTTCGACCCGCGCTCAGTATTGTGGAACACCGAGGTCGGGGTACTGGTGGACAGCCCGGAGCTGGCCGAATACACCCGCGAACTGGCCCAGCAGGGTATGGCGCCTGCGCTGAGCTATCAGGTGAAACTGATAGGTAACAAGCTGGTGTGGGCGACCGAGGACAATGGCCAACGGCACGTGCTGACGTCTGAGCCGGGCGGGATCTGGCGACGCTTCAATGCCTGGATCAGCAAGGCGGTTGGCTTGGAGAAGATGCTATAGCGGC  >  NC_002947/6026404‑6026686

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑