Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 6,026,619 C→T 9.7% G497G (GGC→GGT)  PP_5276 → phospholipase D family protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029476,026,6190CT 9.7% 111.5 / 2.5 41G497G (GGC→GGT) PP_5276phospholipase D family protein
Reads supporting (aligned to +/- strand):  ref base C (19/18);  new base T (2/2);  total (21/20)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.28e-01

AGCTGGCCGAATACACCCGCGAACTGGCCCAGCAGGGTATGGCGCCTGCGCTGAGCTATCAGGTGAAACTGATAGGTAACAAGCTGGTGTGGGCGACCGAGGACAATGGCCAACGGCACGTGCTGACGTCTGAGCCGGGCGGGATCTGGCGACGCTTCAATGCCTGGATCAGCAAGGCGGTTGGCTTGGAGAAGATGCTATAGCGGCGATTCCGGGGCCTTGCGGCCCCCAAAATCATCAGGCAGAAACCGGCTCAAAGGCTCCGCGCCGCCGCGTCAATACAACC  >  NC_002947/6026480‑6026765
                                                                                                                                           |                                                                                                                                                  
aGCTGGCCGAATACACCCGCGAACTGGCCCAGCAGGGTATGGCGCCTGCGCTGAGCTATCAGGTGAAACTGATAGGTAACAAGCTGGTGTGGGCGACCGAGGACAATGGCCAACGGCACGTGCTGACGTCTGAGCCGGGCGGGATCTgg                                                                                                                                           >  7:57579/1‑149 (MQ=255)
       cGAATACACCCGCGAACTGGCCCAGCAGGGTATGGCGCCTGCGCTGAGCTATCAGGTGAAACTGATAGGTAACAAGCTGGTGTGGGCGACCGAGGACAATGGCCAACGGCACGTGCTGACGTCTGAGCCGGGCGGGATCTGGCGACGCt                                                                                                                                    >  1:205797/1‑149 (MQ=255)
           tACACCCGCGAACTGGCCCAGCAGGGTATGGCGCCTGCGCTGAGCTATCAGGTGAAACTGATAGGTAACAAGCTGGTGTGGGCGACCGAGGACAATGGCCAACGGCACGTGCTGACGTCTGAGCCGGGCGGGATCTGGCGACGCTTCaa                                                                                                                                >  8:155260/1‑149 (MQ=255)
             cacCCGCGAACTGGCCCAGCAGGGTATGGCGCCTGCGCTGAGCTATCAGGTGAAACTGATAGGTAACAAGCTGGTGTGGGCGACCGAGGACAATGGCCAACGGCACGTGCTGACGTCTGAGCCGGGCGGGATCTGGCGACGCTTCAATg                                                                                                                              <  2:205797/149‑1 (MQ=255)
                ccGCGAACTGGCCCAGCAGGGTATGGCGCCTGCGCTGAGCTATCAGGTGAAACTGATAGGTAACAAGCTGGTGTGGGCGACCGAGGACAATGGCCAACGGCACGTGCTGACGTCTGAGCCGGGTGGGATCTGGCGACGCTTCAATGCCt                                                                                                                           >  6:187445/1‑149 (MQ=255)
                ccGCGAACTGGCCCAGCAGGGTATGGCGCCTGCGCTGAGCTATCAGGTGAAACTGATAGGTAACAAGCTGGTGTGGGCGACCGAGGACAATGGCCAACGGCACGTGCTGACGTCTGAGCCGGGTGGGATCTGGCGACGCTTCAATGCCt                                                                                                                           >  6:192404/1‑149 (MQ=255)
                     aaCTGGCCCAGCAGGTTATGGCGCCTGCGCTGAGCTATCAGGTGAAACTGATAGGTAACAAGCTGGTGTGGGCGACCGAGGACAATGGCCAACGGCACGTGCTGACGTCTGAGCCGGGCGGGATCTGGCGACGCtt                                                                                                                                   <  8:123434/136‑1 (MQ=255)
                     aaCTGGCCCAGCAGGGTATGGCGCCTGCGCTGAGCTATCAGGTGAAACTGATAGGTAACAAGCTGGTGTGGGCGACCGAGGACAATGGCCAACGGCACGTGCTGACGTCTGAGCCGGGCGGGa                                                                                                                                                <  8:274642/123‑1 (MQ=255)
                     aaCTGGCCCAGCAGGGTATGGCGCCTGCGCTGAGCTATCAGGTGAAACTGATAGGTAACAAGCTGGTGTGGGCGACCGAGGACAATGGCCAACGGCACGTGCTGACGTCTGAGCCGGGCGGGa                                                                                                                                                >  7:274642/1‑123 (MQ=255)
                     aaCTGGCCCAGCAGGGTATGGCGCCTGCGCTGAGCTATCAGGTGAAACTGATAGGTAACAAGCTGGTGTGGGCGACCGAGGACAATGGCCAACGGCACGTGCTGACGTCTGAGCCGGGCGGGATCTGGCGACGCtt                                                                                                                                   >  7:123434/1‑136 (MQ=255)
                            ccAGCAGGGTATGGCGCCTGCGCTGAGCTATCAGGTGAAACTGATAGGTAACAAGCTGGTGTGGGCGACCGAGGACAATGGCCAACGGCACGTGCTGACGTCTGAGCCGGGCGGGATCTGGCGACGCTTCAATGCCTGGATCAGCAAgg                                                                                                               >  8:161379/1‑149 (MQ=255)
                            ccAGCAGGGTATGGCGCCTGCGCTGAGCTATCAGGTGAAACTGATAGGTAACAAGCTGGTGTGGGCGACCGAGGACAATGGCCAACGGCACGTGCTGACGTCTGAGCCGGGCGGGATCTGGCGACGCTTCAATGCCTGGATCAGCAAgg                                                                                                               <  7:155260/149‑1 (MQ=255)
                                 atggTATGGCGCCTGCGCTGAGCTATCAGGTGAAACTGATAGGTAACAAGCTGGTGTGGGCGACCGAGGACAATGGCCAACGGCACGTGCTGACGTCTGAGCCGGGCGGGATCTGGCGACGCTTCAATGCCTGGATCAGCAAGGCGGtt                                                                                                          <  8:57579/147‑1 (MQ=255)
                                     tATGGCGCCTGCGCTGAGCTATCAGGTGAAACTGATAGGTAACAAGCTGGTGTGGGCGACCGAGGACAATGGCCAACGGCACGTGCTGACGTCTGAGCCGGGCGGGATCTGGCGACGCTTCAATGCCTGGATCAGCAAGGCGGTTGGCt                                                                                                      <  7:161379/149‑1 (MQ=255)
                                         gcgcCTGCGCTGAGCTATCAGGTGAAACTGATAGGTAACAAGCTGGTGTGGGCGACCGAGGACAATGGCCAACGGCACGTGCTGACGTCTGAGCCGGGTGGGATCTGGCGACGCTTCAATGCCTGGATCAGCAAGGCGGTTGGCTTGga                                                                                                  <  5:192404/149‑1 (MQ=255)
                                         gcgcCTGCGCTGAGCTATCAGGTGAAACTGATAGGTAACAAGCTGGTGTGGGCGACCGAGGACAATGGCCAACGGCACGTGCTGACGTCTGAGCCGGGTGGGATCTGGCGACGCTTCAATGCCTGGATCAGCAAGGCGGTTGGCTTGga                                                                                                  <  5:187445/149‑1 (MQ=255)
                                         gcgcCTGCGCTGAGCTATCAGGTGAAACTGATAGGTAACAAGCTGGTGTGGGCGACCGAGGACAATGGCCAACGGCACGTGCTAACGTCTGAGCCGGGCGGGATCTGGCGACGCTTCAATGCCTGGATCAGCAAGGCGGTTGGCTTg                                                                                                    >  3:89455/1‑147 (MQ=255)
                                            ccTGCGCTGAGCTATCAGGTGAAACTGATAGGTAACAAGCTGGTGTGGGCGACCGAGGACAATGGCCAACGGCACGTGCTAACGTCTGAGCCGGGCGGGATCTGGCGACGCTTCAATGCCTGGATCAGCAAGGCGGTTGGCTTGGagaa                                                                                               <  4:89455/149‑1 (MQ=255)
                                                   ttaccTATCAGTTTCACCTGATAGGTAACAAGCTGGTGTGGGCGACCGAGGACAATGGCCAACGGCACGTGCTGACGTCTGAGCCGGGCGGGATCTGGCGACGCTTCAATGCCTGGATCAGCAAGGCGGTTGGCTTGGAGAAGATGCta                                                                                        >  1:249700/5‑149 (MQ=255)
                                                   tGAGCTATCAGGTGAAACTGATAGGTAACAAGCTGGTGTGGGCGACCGAGGACAATGGCCAACGGCACGTGCTGACGTCTGAGCCGGGCGGGATCTGGCGACGCTTCAATGCCTGGATCAGCAAGGCGGt                                                                                                           >  3:94634/1‑130 (MQ=255)
                                                   tGAGCTATCAGGTGAAACTGATAGGTAACAAGCTGGTGTGGGCGACCGAGGACAATGGCCAACGGCACGTGCTGACGTCTGAGCCGGGCGGGATCTGGCGACGCTTCAATGCCTGGATCAGCAAGGCGGt                                                                                                           <  4:94634/130‑1 (MQ=255)
                                                             ggTTAAACTGATAGCTAACAAGCGGGTTTGGGCGACCGAGGACAATGGCCAACGGCACGTGCTGACGTCTGAGCCGGGCGGGATCTGGCGACGCTTCAATGCCTGGATCAGCAAGGCGGTTGGCTTGGAGAAGATGc                                                                                          <  2:50783/137‑1 (MQ=255)
                                                             ggTGAAACTGATAGGTAACAAGCTGGTGTGCGCGACCGAGGACAATGGCCAACGGCACGTGCTGACGTCTGAGCCGGGCGGGATCTGGCGACGCTTCAATGCCTGGATCAGCAAGGCGGTTGGCTTGGAGAAGATGCTAGAtcgga                                                                                 >  1:50783/1‑145 (MQ=255)
                                                                       aTAGGTAACAAGCTGGTGTGGGCGACCGAGGACAATGGCCAACGGCACGTGCTGACGTCTGAGCCGGGCGGGATCTGGCGACGCTTCAATGCCTGGATCAGCAAGGCGGTTGGCTTGGAGAAGATGCTATAGCGGCGATTCCGGGGCCt                                                                    >  1:262179/1‑149 (MQ=255)
                                                                        tAGGTAACAAGCTGGTGTGGGCGACCGAGGACAATGGCCAACGGCACGTGCTGACGTCTGAGCCGGGCGGGATCTGGCGACGCTTCAATGCCTGGATCAGCAAGGCGGtt                                                                                                          <  1:178600/110‑1 (MQ=255)
                                                                        tAGGTAACAAGCTGGTGTGGGCGACCGAGGACAATGGCCAACGGCACGTGCTGACGTCTGAGCCGGGCGGGATCTGGCGACGCTTCAATGCCTGGATCAGCAAGGCGGtt                                                                                                          >  2:178600/1‑110 (MQ=255)
                                                                           gTAACAAGCTGGTGTGGGCGACCGAGGACAATGGCCAACGGCACGTGCTGACGTCTGAGCCGGGCGGGATCTGGCGACGCTTCAATGCCTGGATCAGCAAGGCg                                                                                                             >  4:147775/1‑104 (MQ=255)
                                                                           gTAACAAGCTGGTGTGGGCGACCGAGGACAATGGCCAACGGCACGTGCTGACGTCTGAGCCGGGCGGGATCTGGCGACGCTTCAATGCCTGGATCAGCAAGGCg                                                                                                             <  3:147775/104‑1 (MQ=255)
                                                                                   cTGGTGTGGGCGACCGAGGACAATGGCCAACGGCACGTGCTGACGTCTGAGCCGGGCGGGATCTGGCGACGCTTCAATGCCTGGATCAGCAAGGCGGTTGGCTTGGAGAAGATGCTATAGCGGCGATTCCGGGGCCtt                                                                   <  3:85904/138‑1 (MQ=255)
                                                                                   cTGGTGTGGGCGACCGAGGACAATGGCCAACGGCACGTGCTGACGTCTGAGCCGGGCGGGATCTGGCGACGCTTCAATGCCTGGATCAGCAAGGCGGTTGGCTTGGAGAAGATGCTATAGCGGCGATTCCGGGGCCtt                                                                   >  4:85904/1‑138 (MQ=255)
                                                                                          gggCGACCGAGGACAATGGCCAACGGCACGTGCTGACGTCTGAGCCGGGCGGGATCTGGCGACGCTTCAATGCCTGGATCAGCAAGGCGGTTGGCTTGGAGAAGATGCTATAGCGGCGATTCCGGGGCCTTGCGGCCCCCAAAatcatc                                                 <  4:293230/149‑1 (MQ=255)
                                                                                           ggCGACCGAGGACAATGGCCAACGGCACGTGCTGACGTCTGAGCCGGGCGGGATCTGGCGACGCTTCAATGCCTGGATCAGCAAGGCGGTTGGCTTGGAGAAGATGCTATAGCGGCGATTCCGGGGCCTTGCGGCCCCCAAAAtcatca                                                <  2:262179/149‑1 (MQ=255)
                                                                                                ccGAGGACAATGGCCAACGGCACGTGCTGACGTCTGAGCCGGGCGGGATCTGGCGACGCTTCAATGCCTGGATCAGCAAGGCGGTTGGCTTGGAGAAGATGCTATAGCGGCGATTCCGGGGCCTTGCGGCCCCCAAAAtcatca                                                <  2:113916/144‑1 (MQ=255)
                                                                                                ccGAGGACAATGGCCAACGGCACGTGCTGACGTCTGAGCCGGGCGGGATCTGGCGACGCTTCAATGCCTGGATCAGCAAGGCGGTTGGCTTGGAGAAGATGCTATAGCGGCGATTCCGGGGCCTTGCGGCCCCCAAAAtcatca                                                >  1:113916/1‑144 (MQ=255)
                                                                                                               aaCGGCACGTGCTGACGTCTGAGCCGGGCGGGATCTGGCGACGCTTCAATGCCTGGATCAGCAAGGCGGTTGGCTTGGAGAAGATGCTATAGCGGCGATTCCGGGGCCTTGCGGCCCCCAAAATCATCAGGCAGAAACCGGc                                   >  6:236434/1‑142 (MQ=255)
                                                                                                               aaCGGCACGTGCTGACGTCTGAGCCGGGCGGGATCTGGCGACGCTTCAATGCCTGGATCAGCAAGGCGGTTGGCTTGGAGAAGATGCTATAGCGGCGATTCCGGGGCCTTGCGGCCCCCAAAATCATCAGGCAGAAACCGGc                                   <  5:236434/142‑1 (MQ=255)
                                                                                                                aCGGCACGTGCTGACGTCTGAGCCGGGCGGGATCTGGCGACGCTTCAATGCCTGGATCAGCAAGGCGGTTGGCTTGGAGAAGATGCTATAGCGGCGATTCCGGGGCCTTGCg                                                                >  2:347912/1‑112 (MQ=255)
                                                                                                                aCGGCACGTGCTGACGTCTGAGCCGGGCGGGATCTGGCGACGCTTCAATGCCTGGATCAGCAAGGCGGTTGGCTTGGAGAAGATGCTATAGCGGCGATTCCGGGGCCTTGCg                                                                <  1:347912/112‑1 (MQ=255)
                                                                                                                   gCACGTGCTGACGTCAGAGCCGCGCGGGATCTGGCGACGCTGCAATGCCTGGATCAGCAAGGCGGTTGGCTTGGAGAAGATGCTATAGCGGCGATTCCGGGGCCTTGCGGCCCCCAACATCATCAGGCAGAAACCGGCTCAAAGGCTcc                        >  2:233883/1‑149 (MQ=255)
                                                                                                                     aCGTGCTGACGTCTGAGCCGGGCGGGATCTGGCGACGCTTCAATGCCTGGATCAGCAAGGCGGTTGGCTTGGAGAAGATGCTATAGCGGCGATTCCGGGGCCTTGCGGCCCCCAAAATCATCAGGCAGAAACCGGCTCAAAGGCTCcgc                      >  8:293224/1‑149 (MQ=255)
                                                                                                                              cGTCTGAGCCGGGCGGGATCTGGCGACGCTTCAATGCCTGGATCAGCAAGGCGGTTGGCTTGGAGAAGATGCTATAGCGGCGATTCCGGGGCCTTGCGGCCCCCAAAATCATCAGGCAGAAACCGGCTCAAAGGCTCCGCGCCGCcgcg             <  2:249700/149‑1 (MQ=255)
                                                                                                                                         ggcgggATCTGGCGACGCTTCAATGCCTGGATCAGCAAGGCGGTTGGCTTGGAGAAGATGCTATAGCGGCGATTCCGGGGCCTTGCGGCCCCCAAAATCATCAGGCAGAAACCGGCTCAAAGGCTCCGCGCCGCCGCGTCAATAcaacc  <  1:233883/149‑1 (MQ=255)
                                                                                                                                           |                                                                                                                                                  
AGCTGGCCGAATACACCCGCGAACTGGCCCAGCAGGGTATGGCGCCTGCGCTGAGCTATCAGGTGAAACTGATAGGTAACAAGCTGGTGTGGGCGACCGAGGACAATGGCCAACGGCACGTGCTGACGTCTGAGCCGGGCGGGATCTGGCGACGCTTCAATGCCTGGATCAGCAAGGCGGTTGGCTTGGAGAAGATGCTATAGCGGCGATTCCGGGGCCTTGCGGCCCCCAAAATCATCAGGCAGAAACCGGCTCAAAGGCTCCGCGCCGCCGCGTCAATACAACC  >  NC_002947/6026480‑6026765

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑