Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 6,080,139 T→G 5.1% I28S (ATC→AGC) ‡ PP_5332 → hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029476,080,1390TG 5.1% 249.4 / 3.7 78I28S (ATC→AGC) ‡PP_5332hypothetical protein
Reads supporting (aligned to +/- strand):  ref base T (36/38);  new base G (2/2);  total (38/40)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.51e-01

TGGCGGTAAACTCATTGCATCTGCCCGGGTCCAAGGCCCACTGGCAATCAATGAGAAGAACGCAATGGCTACCTTTCAGGTCGTAACCGAGCAACATGGCGAA‑‑CTCAACTGCTGGCGTATCAGCAGTGACCGTGCCGAACTCCTGATCGCCCAGCAGGGCGCACAGGTCCTCAGCTACCAGCAGGTAGGCGAACCGCCGCTGCTGTGGCTGAGCGACCAGGCCATCTTCCGCCAGGGCAAGTCGGTACGCGCCGGGGTACCGGTGTGCTGGCCATGGTTTGGCAACCTGCAGC  >  NC_002947/6079993‑6080285
                                                                                                                                                    |                                                                                                                                                  
tGGCGGTAAACTCATTGCATCTGCCCGGGTCCAAGGCCCACTGGCAATCAATGAGAAGAACGCAATGGCTACCTTTCAGGTCGTAACCGAGCAACATGGCGAA‑‑CTCAACTGCTGGCGTATCAGCAGTGACCGTGCCGAACTCCTGATCg                                                                                                                                                  >  3:195562/1‑149 (MQ=255)
     gTAAACTCATTGCATCTGCCCGGGTCCAAGGCCCACTGGCAATCAATGAGAAGAACGCAATGGCTACCTTTCAGGTCGTAACCGAGCAACATGGCGAA‑‑CTCAACTGCTGGCGTATCAGCAGTGACCGTGCCGAACTCCTGATCGCCcag                                                                                                                                             >  2:161811/1‑149 (MQ=255)
      tAAACTCATTGCATCTGCCCGGGTCCAAGGCCCACT‑GCAATCAATGAGAAGAACGCAATGGCTACCTTTCAGGTCGTAACCGAGCAACATGGCGAA‑‑CTCAACTGCTGGCGTATCAGCAGTGACCGTGCCGAACTCCTGATCGCCcag                                                                                                                                             >  8:82739/1‑147 (MQ=255)
        aaCTCATTGCATCTGCCCGGGTCCAAGGCCCACTGGCAATCAATGAGAAGAACGCAATGGCTACCTTTCAGGTCGTAACCGAGCAACATGGCGAA‑‑CTCAACTGCTGGCGTATCAGCAGTGACCGTGCCGAACTCCTGATCGCCcagcag                                                                                                                                          <  8:222072/149‑1 (MQ=255)
           tCATTGCATCTGCCCGGGTCCAAGGCCCACTGGCAATCAATGAGAAGAACGCAATGGCTACCTTTCAGGTCGTAACCGAGCAACATGGCGAA‑‑CTCAACTGCTGGCGTATCAGCAGTGACCGTGCCGAACTCCTGTGCGCCCTGCTGGgc                                                                                                                                       >  1:179733/1‑149 (MQ=255)
                 cATCTGCCCGGATCCAAGGCCCACTGGCAATCAATGAGAAGAACGCAATGGCTACCTTTCAGGTCGTAACCGAGCAACATGGCGAA‑‑CTCAACTGCTGGCGTATCAGCAGTGACCGTGCCGAACTCCTGTGCGCCCTGCTGGgcgatc                                                                                                                                   <  8:297013/147‑4 (MQ=255)
                 cATCTGCCCGGATCCAAGGCCCACTGGCAATCAATGAGAAGAACGCAATGGCTACCTTTCAGGTCGTAACCGAGCAACATGGCGAA‑‑CTCAACTGCTGGCGTATCAGCAGTGACCGTGCCGAACTCCTGTGCGCCCTGCTGGgcgatc                                                                                                                                   >  7:297013/1‑144 (MQ=255)
                  aTCTGCCCGGGTCCAAGGCCCACTGGCAATCAATGAGAAGAACGCAATGGCTACCTTTCAGGTCGTAACCGTGCAACATGGCGAA‑‑CTCAACTGCTGGCGTATCAGCAGTGACCGTGCCGAACTCCTGATCGCCCAGCAGGGCGCACAgg                                                                                                                                >  4:9665/1‑149 (MQ=255)
                     tGCCCGGGTCCAAGGCCCACTGGCAATCAATGAGAAGAACGCAATGGCTACCTTTCAGGTCGTAACCGAGCAACATGGCGAA‑‑CTCAACTGCTGGCGTATCAGCAGTGACCGTGCCGAACTCCTGATCGCCCAGCAGGGCGCACAGGTcc                                                                                                                             >  8:86008/1‑149 (MQ=255)
                       cccGGGTCCAAGGCCCACTGGCAATCAATGAGAAGAACGCAATGGCTACCTTTCAGGTCGTAACCGAGCAACATGGCGAA‑‑CTCAACTGCTGGCGTATCAGCAGTGACCGTGCCGAACTCCTGTGCGCCCTGCTGGGCGATCAgga                                                                                                                               <  2:179733/145‑2 (MQ=255)
                       cccGGGTCCAAGGCCCACTGGCAATCAATGAGAAGAACGCAATGGCTACCTTTCAGGTCGTAACCGAGCAACATGGCGAA‑‑CTCAACTGCTGGCGTATCAGCAGTGACCGTGCCGAACTCCTGATCGCCCAGCAGGGCGCACAGGTCCt                                                                                                                            >  2:255025/1‑148 (MQ=255)
                       cccGGGTCCAAGGCCCACTGGCAATCAATGAGAAGAACGCAATGGCTACCTTTCAGGTCGTAACCGAGCAACATGGCGAA‑‑CTCAACTGCTGGCGTATCAGCAGTGACCGTGCCGAACTCCTGATCGCCCAGCAGGGCGCACAGGTCCTc                                                                                                                           <  4:195562/149‑1 (MQ=255)
                       cccGGGTCCAAGGCCCACTGGCAATCAATGAGAAGAACGCAATGGCTACCTTTCAGGTCGTAACCGAGCAACATGGCGAA‑‑CTCAACTGCTGGCGTATCAGCAGTGACCGTGCCGAACTCCTGATCGCCCAGCAGGGCGCACAGGTCCTc                                                                                                                           <  7:86008/149‑1 (MQ=255)
                        ccGGGTCCAAGGCCCACTGGCAATCAATGAGAAGAACGCAATGGCTACCTTTCAGGTCGTAACCGAGCAACATGGCGAA‑‑CTCAACTGCTGGCGTATCAGCAGTGACCGTGCCGAACTCCTGATCGCCCAGCAGGGCGCACAGGTCCt                                                                                                                            >  2:139983/1‑147 (MQ=255)
                        ccGGGTCCAAGGCCCACTGGCAATCAATGAGAAGAACGCAATGGCTACCTTTCAGGTCGTAACCGAGCAACATGGCGAA‑‑CTCAACTGCTGGCGTATCAGCAGTGACCGTGCCGAACTCCTGATCGCCCAGCAGGGCGCACAGGTCCt                                                                                                                            >  1:244999/1‑147 (MQ=255)
                        ccGGGTCCAAGGCCCACTGGCAATCAATGAGAAGAACGCAATGGCTACCTTTCAGGTCGTAACCGAGCAACATGGCGAA‑‑CTCAACTGCTGGCGTATCAGCAGTGACCGTGCCGAACTCCTGATCGCCCAGCAGGGCGCACAGGTCCt                                                                                                                            >  7:298230/1‑147 (MQ=255)
                        ccGGGTCCAAGGCCCACTGGCAATCAATGAGAAGAACGCAATGGCTACCTTTCAGGTCGTAACCGAGCAACATGGCGAA‑‑CTCAACTGCTGGCGTATCAGCAGTGACCGTGCCGAACTCCTGATCGCCCAGCAGGGCGCACAGGTCCTCa                                                                                                                          <  3:99804/149‑1 (MQ=255)
                          gggTCCAAGGCCCACTGGCAATCAATGAGAAGAACGCAATGGCTACCTTTCAGGTCGTAACCGAGCAACATGGCGAA‑‑CTCAACTGCTGGCGTATCAGCAGTGACCGTGCCGAACTCCTGAt                                                                                                                                                    <  7:60967/121‑1 (MQ=255)
                          gggTCCAAGGCCCACTGGCAATCAATGAGAAGAACGCAATGGCTACCTTTCAGGTCGTAACCGAGCAACATGGCGAA‑‑CTCAACTGCTGGCGTATCAGCAGTGACCGTGCCGAACTCCTGAt                                                                                                                                                    >  8:60967/1‑121 (MQ=255)
                            gTCCAAGGCCCACT‑GCAATCAATGAGAAGAACGCAATGGCTACCTTTCAGGTCGTAACCGAGCAACATGGCGAA‑‑CTCAACTGCTGGCGTATCAGCAGTGACCGTGCCGAACTCCTGATCGCCCAGCAGGGCGCACAGGTCCTCAGCTAc                                                                                                                     <  7:82739/149‑1 (MQ=255)
                                 aGGCCCACTGGCAATCAATGAGAAGAACGCAATGGCTACCTTTCAGGTCGTAACCGAGCAACATGGCGAA‑‑CTCAACTGCTGGCGTATCAGCAGTGACCGTGCCGAACTCCTGATCGCCCAGCAGGGCGCACAGGTCATCAGCTACcagc                                                                                                                 <  6:100254/149‑1 (MQ=255)
                                   gCCCACTGGCAATCAATGAGAAGAACGCAATGGCTACCTTTCAGGTCGTAACCGAGCAACATGGCGAAAGAGCAACTGCTGGCGTATCAGCAGTGACCGTGCCGAACTCCTGATCGCCCAGCAGGGCGCACAGGTCCTCAGCCAcagca                                                                                                                 >  1:282128/1‑145 (MQ=255)
                                    cccACTGGCAATCAATGAGAAGAACGCAATGGCTACCTTTCAGGTCGTAACCGAGCAACATGGCGAA‑‑CTCAACTGCTGGCGTATCAGCAGTGACCGTGCCGAACTCCTGATCGCCCAGCAgg                                                                                                                                         >  8:271951/1‑122 (MQ=255)
                                    cccACTGGCAATCAATGAGAAGAACGCAATGGCTACCTTTCAGGTCGTAACCGAGCAACATGGCGAA‑‑CTCAACTGCTGGCGTATCAGCAGTGACCGTGCCGAACTCCTGATCGCCCAGCAgg                                                                                                                                         <  7:271951/122‑1 (MQ=255)
                                           gCAATCAATGAGAAGAACGCAATGGCTACCTTTCAGGTCGTAACCGAGCAACATGGCGAA‑‑CTCAACTGCTGGCGTATCAGCAGTGACCGTGCCGAACTCCTGATCGCCCAGCAGGGCGCACAGGTCCTCAGCTACCAGCAGGTAGGCg                                                                                                        <  1:161811/148‑1 (MQ=255)
                                           gCAATCAATGAGAAGAACGCAATGGCTACCTTTCAGGTCGTAACCGAGCAACATGGCGAA‑‑CTCAACTGCTGGCGTATCAGCAGTGACCGTGCCGAACTCCTGATCGCCCAGCAGGGCGCACAGGTCCTCAGCTACCAGCAGGTAGGCg                                                                                                        >  3:131347/1‑148 (MQ=255)
                                           gCAATCAATGAGAAGAACGCAATGGCTACCTTTCAGGTCGTAACCGAGCAACATGGCGAA‑‑CTCAACTGCTGGCGTATCAGCAGTGACCGTGCCGAACTCCTGATCGCCCAGCAGGGCGCACAGGTCCTCAGCTACCAGCAGGTAGGCg                                                                                                        >  2:285562/1‑148 (MQ=255)
                                           gCAATCAATGAGAAGAACGCAATGGCTACCTTTCAGGTCGTAACCGACCAAAATGGCGAA‑‑CTCAACTGCTGGCGTATCAGCAGTGACCGTGCCGAACTCCTGATCGACCAGCAGGGCGCACAGCTCCTCAGCTACCAGCACGTAGGCg                                                                                                        >  7:282516/1‑148 (MQ=255)
                                             aatcaatGAGAAGAACGCAATGGCTACCTTTCAGGTCGTAACCGAGCAACATGGCGAA‑‑CTCAACTGCTGGCGTATCAGCAGTGACCGTGCCGAACTCCTGATCGCCCAGCAGGGCGCACAGGTCCTCAGCTACCAGCAGGTAGGCGAAc                                                                                                     <  1:255025/149‑1 (MQ=255)
                                             aatcaatGAGAAGAACGCAATGGCTACCTTTCAGGTCGTAACCGAGCAACATGGCGAA‑‑CTCAACTGCTGGCGTATCAGCAGTGACCGTGCCGAACTCCTGATCGCCCAGCAGGGCGCACAGGTCCTCAGCTACCAGCAGGTAGGCGAAc                                                                                                     <  6:29680/149‑1 (MQ=255)
                                               tcaatGAGAAGAACGCAATGGCTACCTTTCAGGTCGTAACCGAGCAACATGGTGAA‑‑CTCAACTGCTGGCGTATCAGCAGTGACCGTGCCGAACTCCTGATCGCCCAGCAGGGCGCACAGGTCCTCAGCTACCAGCAGGTAGGCGAAccg                                                                                                   >  6:308789/1‑149 (MQ=255)
                                                   tGAGAAGAACGCAATGGCTACCTTTCAGGTCGTAACCGAGCAACATGGCGAA‑‑CTCAACTGCTGGCGTATCAGCAGTGACCGTGCCGAACTCCTGATCGCCCAGCAGGGCGCACAGGTCCTCAGCTACCAGCAGGTAGGCGAACcgccgc                                                                                               >  3:305656/1‑149 (MQ=255)
                                                     agaagaACGCAATGGCTACCTTTCAGGTCGTAACCGAGCAACATGGCGAA‑‑CTCAACTGCTGGCGTATCAGCAGTGACCGTGCCGAACTCCTGATCGCCCAGCAGGGCGCACAGGTCCTCAGCTACCAGCAGGTAGGCGAACCGCCgctg                                                                                             >  6:97500/1‑149 (MQ=255)
                                                       aagaaCGCAATGGCTACCTTTCAGGTCGTAACCGAGCAACATGGTGAA‑‑CTCAACTGCTGGCGTATCAGCAGTGACCGTGCCGAACTCCTGATCGCCCAGCAGGGCGCACAGGTCCTCAGCTACCAGCAGGTAGGCGAACCGCCgctgct                                                                                           <  5:308789/149‑1 (MQ=255)
                                                       aagaaCGCAATGGCTACCTTTCAGGTCGTAACCGAGCAACATGGCGAA‑‑CTCAACTGCTGGCGTATCAGCAGTGACCGTGCCGAACTCCTGATCGCCCAGCAGGGCGCACAGGTCCTCAGCTACCAGCAGGTAGGCGAACCGCCgctgct                                                                                           >  5:246322/1‑149 (MQ=255)
                                                       aagaaCGCAATGGCTACCTTTCAGGTCGTAACCGAGCAACATGGCGAA‑‑CTCAACTGCTGGCGTATCAGCAGTGACCGTGCCGAACTCCTGATCGCCCAGCAGGGCGCACAGGTCCTCAGCTACCAGCAGGTAGGCGAACCGCCgctgct                                                                                           >  6:124330/1‑149 (MQ=255)
                                                        agaaCGCAATGGCTACCTTTCATGTCGTAACCGAGCAACATGGCGAA‑‑CTCAACTGCTGGCGTATCAGCAGTGACCGTGCCGAACTCCTGATCGCCCAGCAGGGCGCACAGGTCCTCAGCTACCAGCAGGTAGGCGAACCGCCGctgctg                                                                                          <  6:246322/149‑1 (MQ=255)
                                                           aCGCAATGGCTACCTTTCAGGTCGTAACCGTGCAACATGGCGAA‑‑CTCAACTGCTGGCGTATCAGCAGTGACCGTGCCGAACTCCTGATCGCCCAGCAGGGCGCACAGGTCCTCAGCTACCAGCAGGTAGGCGAACCGCCGCTGCTGTgg                                                                                       <  3:9665/149‑1 (MQ=255)
                                                            cGCAATGGCTACCTTTCAGGTCGTAACCGAGCAACATGGCGAA‑‑CTCAACTGCTGGCGTATCAGCAGTGACCGTGCCGAACTCCTGATCGCCCAGCAGGGCGCACAGGTCCTCAGCTACCAGCAGGTAGGCGAACCGCCGCTGCTGTGGc                                                                                      <  1:139983/149‑1 (MQ=255)
                                                            cGCAATGGCTACCTTTCAGGTCGTAACCGAGCAACATGGCGAA‑‑CTCAACTGCTGGCGTATCAGCAGTGACCGTGCCGAACTCCTGATCGCCCAGCAGGGCGCACAGGTCCTCAGCTACCAGCAGGTAGGCGAACCGCCGCTGCTGTGGc                                                                                      <  4:305656/149‑1 (MQ=255)
                                                             gCAATGGCTACCTTTCAGGTCGTAACCGAGCAACATGGCGAA‑‑CTCAACTGCTGGCGTATCAGCAGTGACCGTGCCGAACTCCTGATCGCCCAGCAGGGCGCACAGGTCCTCAGCTACCAGCAGGTAGGCGAACCGCCGCTGCTGTGGCt                                                                                     <  8:298230/149‑1 (MQ=255)
                                                               aaTGGCTACCTTTCAGGTCGTAACCGAGCAACATGGCGAA‑‑CTCAACTGCTGGCGTATCAGCAGTGACCGTGCCGAACTCCTGATCGCCCAGCAGGGCGCACAGGTCCTCAGCTACCAGCAGGTAGGCGAACCGCCGCTGCTGTGGCTGa                                                                                   <  5:124330/149‑1 (MQ=255)
                                                               aaTGGCTACCTTTCAGGTCGTAACCGAGCAACATGGCGAA‑‑CTCAACTGCTGGCGTATCAGCAGTGACCGTGCCGAACTCCTGATCGCCCAGCAGGGCGCACAGGTCCTCAGCTACCAGCAGGTAGGCGAACCGCCGCTGCTGTGGCTGa                                                                                   <  5:97500/149‑1 (MQ=255)
                                                                 tGGCTACCTTTCAGGTCGTAACCGAGCAACATGGCGAA‑‑CTCAACTGCTGGCGTATCAGCAGTGACCGTGCCGAACTCCTGATCGCCCAGCAGGGCGCGCAGGTCCTCAGCTACCAGCAGGTAGGCGAACCGCCGCTGCTGTGGCTGAGc                                                                                 >  7:198766/1‑149 (MQ=255)
                                                                   gCTACCTTTCAGGTCGTAACCGAGCAACATGGCGAA‑‑CTCAACTGCTGGCGTATCAGCAGTGACCGTGCCGAACTCCTGATCGCCCAGCAGGGCGCACAGGTCCTCAGCTACCAGCAGGTAGGCGAACCGCCGCTGCTGTGGCTGAGCGa                                                                               <  1:285562/149‑1 (MQ=255)
                                                                      aCCTTTCAGGTCGTAACCGAGCAACATGGCGAA‑‑CTCAACTGCTGGCGTATCAGCAGTGACCGTGCCGAACTCCTGATCGCCCAGCAGGGCGCACAGGTCCTCAGCTACCAGCAGGTAGGCGAACCGCCGCTGCTGTGGCTGAGCGAc                                                                              >  1:308773/1‑147 (MQ=255)
                                                                      aCCTTTCAGGTCGTAACCGAGCAACATGGCGAA‑‑CTCAACTGCTGGCGTATCAGCAGTGACCGTGCCGAACTCCTGATCGCCCAGCAGGGCGCACAGGTCCTCAGCTACCAGCAGGTAGGCGAACCGCCGCTGCTGTGGCTGAGCGAc                                                                              >  1:13879/1‑147 (MQ=255)
                                                                             aGGTCGTAACCGAGCAACATGGCGAA‑‑CTCAACTGCTGGCGTATCAGCAGTGACCGTGCCGAACTCCTGATCGCCCAGCAGGGCGCACAGGTCCTCAGCTACCAGCAGGTAGGCGAACCGCCGCTGCTGTGGCTGAGCGACCAGGCCATc                                                                     <  2:263767/149‑1 (MQ=255)
                                                                             aGGTCGTAACCGAGCAACATGGCGAA‑‑CTCAACTGCTGGCGTATCAGCAGTGACCGTGCCGAACTCCTGATCGCCCAGCAGGGCGCACAGGTCCTCAGCTACCAGCAGGTAGGCGAACCGCCGCTGCTGTGGCTGAGCGACCAGGCCATc                                                                     >  1:263767/1‑149 (MQ=255)
                                                                                  gTAACCGAGCAACATGGCGAA‑‑CTCAACTGCTGGCGTATCAGCAGTGACCGTGCCGAACTCCTGATCGCCCAGCAGGGCGCACAGGTCCTCAGCTACCAGCAGGTAGGCGAACCGCCGCTGCTGTGGCTGAGCGACCAGGCCATCTTcc                                                                 <  4:131347/148‑1 (MQ=255)
                                                                                       cGAGCAACATGGCGAA‑‑CTCAACTGCTGGCGTATCAGCAGTGACCGTGCCGAACTCCTGATCGCCCAGCAGGGCGCACAGGTCCTCAGCTACCAGCAGGTAGGCGAACCGCCGCTGCTGTGGCTGAGCGACCAGGCCATCTTCCGCCAgg                                                           <  2:13879/149‑1 (MQ=255)
                                                                                             aCATGGCGAA‑‑CTCAACTGCTGGCGTATCAGCAGTGACCGTGCCGAACTCCTGATCGCCCAGCAGGGCGCGCAGGTCCTCAGCTACCAGCAGGTAGGCGAACCGCCGCTGCTGTGGCTGAGCGACCAGGCCATCTTCCGCCAGGGCAAGt                                                     <  8:198766/149‑1 (MQ=255)
                                                                                             aCATGGCGAA‑‑CTCAACTGCTGGCGTATCAGCAGTGACCGTGCCGAACTCCTGATCGCCCAGCAGGGCGCACAGGTCCTCAGCTACCAGCAGGTAGGCGAACCGCCGCTGCTGTGGCTGAGCGACCAGGCCATCTTcc                                                                 >  5:114736/1‑137 (MQ=255)
                                                                                             aCATGGCGAA‑‑CTCAACTGCTGGCGTATCAGCAGTGACCGTGCCGAACTCCTGATCGCCCAGCAGGGCGCACAGGTCCTCAGCTACCAGCAGGTAGGCGAACCGCCGCTGCTGTGGCTGAGCGACCAGGCCATCTTcc                                                                 <  6:114736/137‑1 (MQ=255)
                                                                                                          tCAACTGCTGGCGTATCAGCAGTGACCGTGCCGAACTCCTGATCGCCCAGCAGGGCGCACAGGTCCTCAGCTACCAGCAGGTAGGCGAACCGCCGCTGCTGTGGCTGAGCGACCAGGCCATCTTCCGCCAg                                                            <  6:72272/131‑1 (MQ=255)
                                                                                                          tCAACTGCTGGCGTATCAGCAGTGACCGTGCCGAACTCCTGATCGCCCAGCAGGGCGCACAGGTCCTCAGCTACCAGCAGGTAGGCGAACCGCCGCTGCTGTGGCTGAGCGACCAGGCCATCTTCCGCCAg                                                            >  5:72272/1‑131 (MQ=255)
                                                                                                            aaCTGCTGGCGTATCAGCAGTGACCGTGCCGAACTCCTGATCGCCCAGCAGGGCGCACAGGTCCTCAGCTACCAGCAGGTAGGCGAACCGCCGCTGCTGTGGCTGAGCGACCAGGCCATCTTCCGCCAGGGCAAGTCGGTACGCGCCgg                                        <  2:164705/149‑1 (MQ=255)
                                                                                                             aCTGCTGGCGTATCAGCAGTGACCGTGCCGAACTCCTGATCGCCCAGCAGGGCGCACAGGTCCTCAGCTACCAGCAGGTAGGCGAACCGCCGCTGCTGTGGCTGAGCGACCAGGCCATCTTCCGCCAGGGCAAGTCGGTACGCGCCggg                                       >  5:282181/1‑149 (MQ=255)
                                                                                                              ctgctgGCGTATCAGCAGTGACCGTGCCGAACTCCTGATCGCCCAGCAGGGCGCACAGGTCCTCAGCTACCAGCAGGTAGGCGAACCGCCGCTGCTGTGGCTGAGCGACCAGGCCATCTTCCGCCAGGGCAAGTCGGTACGCGCCgggg                                      <  6:282181/149‑1 (MQ=255)
                                                                                                                  tgGCGTATCAGCAGTGACCGTGCCGAACTCCTGATCGCCCAGCAGGGCGCACAGGTCCTCAGCTACCAGCAGGTAGGCGAACCGCCGCTGCTGTGGCTGAGCGACCAGGcc                                                                        >  6:23826/1‑111 (MQ=255)
                                                                                                                  tgGCGTATCAGCAGTGACCGTGCCGAACTCCTGATCGCCCAGCAGGGCGCACAGGTCCTCAGCTACCAGCAGGTAGGCGAACCGCCGCTGCTGTGGCTGAGCGACCAGGcc                                                                        <  5:23826/111‑1 (MQ=255)
                                                                                                                    gCGTATCAGCAGTGACCGTGCCGAACTCCTGATCGCCCAGCAGGGCGCACAGGTCCTCAGCTACCAGCAGGTAGGCGAACCGCCGCTGCTGTGGCTGAGCGACCAGGCCATCTTCCGCCAggg                                                          >  4:41024/1‑123 (MQ=255)
                                                                                                                    gCGTATCAGCAGTGACCGTGCCGAACTCCTGATCGCCCAGCAGGGCGCACAGGTCCTCAGCTACCAGCAGGTAGGCGAACCGCCGCTGCTGTGGCTGAGCGACCAGGCCATCTTCCGCCAggg                                                          <  3:41024/123‑1 (MQ=255)
                                                                                                                    gCGTATCAGCAGTGACCGTGCCGAACTCCTGATCGCCCAGCAGGGCGCACAGGTCCTCAGCTACCAGCAGGTAGGCGAACCGCCGCTGCTGTGGCTGAGCGACCAGGCCATCTTCCGCCAGGGCAAGTCGGTACGCGCCGGGGTACCgg                                >  7:208120/1‑149 (MQ=255)
                                                                                                                          cagcagTGACCGTGCCGAACTCCTGATCGCCCAGCAGGGCGCACAGGTCCTCAGCTACCAGCAGGTAGGCGAACCGCCGCTGCTGTGGCTGAGCGACCAGGCCATCTTCCGCCAGGGCAAGTCGGTAc                                               >  5:331022/1‑128 (MQ=255)
                                                                                                                          cagcagTGACCGTGCCGAACTCCTGATCGCCCAGCAGGGCGCACAGGTCCTCAGCTACCAGCAGGTAGGCGAACCGCCGCTGCTGTGGCTGAGCGACCAGGCCATCTTCCGCCAGGGCAAGTCGGTAc                                               <  6:331022/128‑1 (MQ=255)
                                                                                                                           agcagTGACCGTGCCGAACTCCTGATCGCCCAGCAGGGCGCACAGGTCCTCAGCTACCAGCAGGTAGGCGAACCGCCGCTGCTGTGGCTGAGCGACCAGGCCATCTTCCGCCAGGGCAAGTCGGTAc                                               <  6:73057/127‑1 (MQ=255)
                                                                                                                           agcagTGACCGTGCCGAACTCCTGATCGCCCAGCAGGGCGCACAGGTCCTCAGCTACCAGCAGGTAGGCGAACCGCCGCTGCTGTGGCTGAGCGACCAGGCCATCTTCCGCCAGCGCAAGTCGGTAc                                               >  5:73057/1‑127 (MQ=255)
                                                                                                                            gcagTGACCGTGCCGAACTCCTGATCGCCCAGCAGGGCGCACAGGTCCTCa                                                                                                                          <  3:310728/51‑1 (MQ=255)
                                                                                                                            gcagTGACCGTGCCGAACTCCTGATCGCCCAGCAGGGCGCACAGGTCCTCa                                                                                                                          >  4:310728/1‑51 (MQ=255)
                                                                                                                            gcagTGACCGTGCCGAACTCCTGATCGCCCAGCAGGGCGCACAGGTCCTCAGCTACCAGCAGGTAGGCGAACCGCCGCTGCTGTGGCTGAGCGAc                                                                              <  4:58223/95‑1 (MQ=255)
                                                                                                                                    cGTGCCGAACTCCTGATCGCCCAGCAGGGCGCACAGGTCCTCAGCTACCAGCAGGTAgg                                                                                                          >  6:878/1‑59 (MQ=255)
                                                                                                                                      tGCCGAACTCCTGATCGCCCAGCAGGGCGCACAGGTCCTCAGCTACCAGCAGGTAGGCGAACCGCCGCTGCTGTGGCTGAGCGACCAGGCCATCTTCCGCCAGGGCAAGTCGGTACGCGCCGGGGTACCGGTGTGCTGGCCATGGTTTg              <  2:33602/149‑1 (MQ=255)
                                                                                                                                      tGCCGAACTCCTGATCGCCCAGCAGGGCGCACAGGTCCTCAGCTACCAGCAGGTAGGCGAACCGCCGCTGCTGTGGCTGAGCGACCAGGCCATCTTCCGCCAGGGCAAGTCGGTACGCGCCGGGGTACCGGTGTGCTGGCCATGGTTTg              <  8:208120/149‑1 (MQ=255)
                                                                                                                                           aaCTCCTGATCGCCCAGCAGGGCGCACAGGTCCTCAGCTACCAGCAGGTAGGCGAACCGCCGCTGCTGTGGCTGAGCGACCAGGCCATCTTCCGCCAGGGCAAGTCGGTACGCGCCGGGGTACCGGTGTGCTGGCCATGGTTTGGCAAc         <  2:308773/149‑1 (MQ=255)
                                                                                                                                                  gATCGCCCAGCAGGGCGCACAGGTCCTCAGCTACCAGCAGGTAGGCGAACCGCCGCTGCTGTGGCTGAGCGACCAGGCCATCTTCCGCCAGGGCAAGTCGGTACGCGCCGGGGTACCGGTGTGCTGGCCATGGTTTGGCAACCTGCAgc  >  8:98512/1‑149 (MQ=255)
                                                                                                                                                  gATCGCCCAGCAGGGCGCACAGGTCCTCAGCTACCAGCAGGTAGGCGAACCGCCGCTGCTGTGGCTGAGCGACCAGGCCATCTTCCGCCAGGGCAAGTCGGTACGCGCCGGGGTACCGGTGTGCTGGCCATGGTTTGGCAACCTGCAgc  >  3:27682/1‑149 (MQ=255)
                                                                                                                                                   aTCGCCCAGCAGGGCGCACAGGTCCTCAGCTACCAGCAGGTAGGCGAACCGCCGCTGCTGTGGCTGAGCGACCAGGCCATCTTCCGCCAGGGCAAGTCGGTACGCGCCGGGGTACCGGTGTGCTGGCCATGGTTTg              >  6:148949/1‑136 (MQ=255)
                                                                                                                                                   aTCGCCCAGCAGGGCGCACAGGTCCTCAGCTACCAGCAGGTAGGCGAACCGCCGCTGCTGTGGCTGAGCGACCAGGCCATCTTCCGCCAGGGCAAGTCGGTACGCGCCGGGGTACCGGTGTGCTGGCCATGGTTTg              <  5:148949/136‑1 (MQ=255)
                                                                                                                                                   aTCGCCCAGCAGGGCGCACAGGTCCTCAGCTACCAGCAGGTAGGCGAACCGCCGCTGCTGTGGCTGAGCGACCAGGCCATCTTCCGCCAGGGCAAGTCGGTACGCGCCGGGGTACCGGTGTGCTGGCCATGGTTTGGCAACCTGCAg   <  2:244999/147‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                  
TGGCGGTAAACTCATTGCATCTGCCCGGGTCCAAGGCCCACTGGCAATCAATGAGAAGAACGCAATGGCTACCTTTCAGGTCGTAACCGAGCAACATGGCGAA‑‑CTCAACTGCTGGCGTATCAGCAGTGACCGTGCCGAACTCCTGATCGCCCAGCAGGGCGCACAGGTCCTCAGCTACCAGCAGGTAGGCGAACCGCCGCTGCTGTGGCTGAGCGACCAGGCCATCTTCCGCCAGGGCAAGTCGGTACGCGCCGGGGTACCGGTGTGCTGGCCATGGTTTGGCAACCTGCAGC  >  NC_002947/6079993‑6080285

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑