Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 6,128,789 A→G 7.1% intergenic (‑905/‑17) PP_5375 ← / → PP_5376 LysR family transcriptional regulator/hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029476,128,7890AG 7.1% 158.5 / 2.3 56intergenic (‑905/‑17)PP_5375/PP_5376LysR family transcriptional regulator/hypothetical protein
Reads supporting (aligned to +/- strand):  ref base A (28/24);  new base G (2/2);  total (30/26)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.57e-01

TCTCAGCAACAATCCTTCTAGCAGTATAGGCTGCCCATTAGGCATCACTCTTGCCAGGTGTCAGCTTTGCACCGTCTGCGACTGACAAAGATGTAATCCACAGATCACCGGGGAGACAGGTGCTGCCCCCTAAGATTCAGTCACACCAAATCGGAGTGACGAACATGAAAGCTCTCAACGGCATTGCCGCCTTACTGATTCTGATGGCTTCCTCGGCCGCGATGGCTGAAGGTGGATCTGACCGCCTGCACGGGAAGATGATTCAGGCGAATGAACAGGCCATGCGCGCATAC  >  NC_002947/6128642‑6128934
                                                                                                                                                   |                                                                                                                                                 
tctcAGCAACAATCCTTCTAGCAGTATAGGCTGCCCATTAGGCATCACTCTTGCCAGGTGTCAGCTTTGCACCGTCTGCGACTGACAAAGATGTAATCCACAGATCACCGGGGAGACAGGTGCTGCCCCCTAAGATTCAGTCACACCa                                                                                                                                                   >  3:88627/1‑148 (MQ=255)
  tcAGCAACAATCCTTCTAGCAGTATAGGCTGCCCATTAGGCATCACTCTTGCCAGGTGTCAGCTTTGCACCGTCTGCGACTGACAAAGATGTAATCCACAGATCACCGGGGAGACAGGTGCTGCCCCCTAAGATTCAGTCACACCAAAt                                                                                                                                                >  3:60487/1‑149 (MQ=255)
      gatctaTCCTTCTAGCAGTATAGGCTGCACATTAGGCATCACTATTGCCATGTGTCAGCTTTGCACCGTCTGCGACTGACAAAGATGTAATCCACAGATCACCGGGGAGACAGGTGCTGCCCCCTAAGATTCAGTCACACCAAATCGGa                                                                                                                                            <  7:159977/144‑1 (MQ=255)
       aacaaTCCTTCTAGCAGTATAGGCTGCCCATTAGGCATCACTCTTTCCAGGTGTCAGCTTTGCACCGTCTGCGACTGACAAAGATGTAATCCACAGATCACCGGGGAGACAGGTGCTGCCCCCTAAGATTCAGTCACACCAAATCGGa                                                                                                                                            <  4:88627/148‑1 (MQ=255)
              cTTCTAGCAGTATAGGCTGCCCATTAGGCATCACTCTTGCCAGGTGTCAGCTTTGCACCGTCTGCGACTTACAAAGATGTAATCCACAGATCACCGGGGAGACAGGTGCTGCCCCCTAAGATTCAGTCACACCAAATCGGAGTGACGaa                                                                                                                                    <  4:331562/149‑1 (MQ=255)
              cTTCTAGCAGTATAGGCTGCCCATTAGGCATCACTCTTGCCAGGTGTCAGCTTTGCACCGTCTGCGACTGACAAAGATGTAATCCACAGATCACCGGGGAGACAGGTGCTGCCCCCTAAGATTCAGTCACACCAAATCGGAGTGACGaa                                                                                                                                    <  2:51122/149‑1 (MQ=255)
                      aGTATAGGCTGCCCATTAGGCATCACTCTTGCCAGGTGTCAGCTTTGCACCGTCTGCGACTGACAAAGATGTAATCCACAGATCACCGGGGAGACAGGTGCTGCCCCCTAAGATTCAGTCACACCAAATCGGAGTGACGAACAt                                                                                                                                 >  2:235175/1‑144 (MQ=255)
                      aGTATAGGCTGCCCATTAGGCATCACTCTTGCCAGGTGTCAGCTTTGCACCGTCTGCGACTGACAAAGATGTAATCCACAGATCACCGGGGAGACAGGTGCTGCCCCCTAAGATTCAGTCACACCAAATCGGAGTGACGAACAt                                                                                                                                 <  1:235175/144‑1 (MQ=255)
                               tGCCCATTAGGCATCACTCTTGCCAGGTGTCAGCTTTGCACCGTCTGCGACTGACAAAGATGTAATCCACAGATCACCGGGGAGACAGGTGCTGCCCCCTAAGATTCAGTCACACCAAATCGGAGTGACGAACATGaaa                                                                                                                             <  1:39793/139‑1 (MQ=255)
                               tGCCCATTAGGCATCACTCTTGCCAGGTGTCAGCTTTGCACCGTCTGCGACTGACAAAGATGTAATCCACAGATCACCGGGGAGACAGGTGCTGCCCCCTAAGATTCAGTCACACCAAATCGGAGTGACGAACATGaaa                                                                                                                             >  2:39793/1‑139 (MQ=255)
                                gCCCATTAGGCATCACTCTTGCCAGGTGTCAGCTTTGCACCGTCTGCGACTGACAAAGATGTAATCCACAGATCACCGGGGAGACAGGTGCTGCCCCCTAAGATTCAGTCACACCAAATCGGAGTGACGAACATGAAAGCTCTCGACgg                                                                                                                  >  8:151182/1‑149 (MQ=255)
                                 cccATTAGGCATCACTCTTGCCAGGTGTCAGCTTTGCACCGTCTGCGACTGACAAAGATGTAATCCACAGATCACCGGGGAGACAGGTGCTGCCCCCTAAGATTCAGTCACACCAAATCGGAGTGACGAACATGAAAGCTCTCAACgg                                                                                                                  >  7:70264/1‑148 (MQ=255)
                                   cATTAGGCATCACTCTTGCCAGGTGTCAGCTTTGCACCGTCTGCGACTGACAAAGATGTAATCCACAGATCACCGGGGAGACAGGTGCTGCCCCCTAAGATTCAGTCACACCAAATCGGAGTGACGAACATGAAAGCTCTCAACGGCAt                                                                                                               >  3:309701/1‑149 (MQ=255)
                                    aTTAGGCATCACTCTTGCCAGGTGTCAGCTTTGCACCGTCTGCGACTGACAAAGATGTAATCCACAGATCACCGGGGAGACAGGTGCTGCCCCCTAAGATTCAGTCACACCAAATCGGAGTGACGAACATGAAAGCTCTCAACGGCAtt                                                                                                              >  1:348832/1‑149 (MQ=255)
                                    aTTAGGCATCACTCTTGCCAGGTGTCAGCTTTGCACCGTCTGCGACTGACAAAGATGTAATCCACAGATCACCGGGGAGACAGGTGCTGCCCCCTAAGATTCAGTCACACCAAATCGGAGTGACGAACATGAAAGCTCTCAACGGCAtt                                                                                                              >  8:77934/1‑149 (MQ=255)
                                           aTCACTCTTGCCAGGTGTCAGCTTTGCACAGTCTGCGACTGACAAAGATGTAATCAACAGATCACCGGGGAGACAGGTGCTGCCCCCTAAGATTCAGTAACACCAAATCGCAGTGACGAACATGAAAGCTATCAACGGCATTGCCGCCt                                                                                                       >  7:288051/1‑149 (MQ=255)
                                              aCTCTTGCCAGGTGTCAGCTTTGCACCGTCTGCGACTGACAAAGATGTAATCCACAGATCACCGGGGAGACAGGTGCTGCCCCCTAAGATTCAGTCACACCAAATCGGAGTGACGAACATGAAAGCTCTCAACGGCATTGCCGCCTTAc                                                                                                    >  1:62887/1‑149 (MQ=255)
                                              aCTCTTGCCAGGTGTCAGCTTTGCACCGTCTGCGACTGACAAAGATGTAATCCACAGATCACCGGGGAGACAGGTGCTGCCCCCTAAGATTCAGTCACACCAAATCGGAGTGACGAACATGAAAGCTCTCAACGGCATTGCCGCCTTAc                                                                                                    <  2:348832/149‑1 (MQ=255)
                                                    gCCAGGTGTCAGCTTTGCACCGTCTGCGACTGACAAAGATGTAATCCACAGATCACCGGGGAGACAGGTGCTGCCCCCTAAGATTCAGTCACACCAAATCGGAGTGACGAACATGAAAGCTCTCAACGGCATTGCCGCCTTAc                                                                                                    <  7:21314/143‑1 (MQ=255)
                                                    gCCAGGTGTCAGCTTTGCACCGTCTGCGACTGACAAAGATGTAATCCACAGATCACCGGGGAGACAGGTGCTGCCCCCTAAGATTCAGTCACACCAAATCGGAGTGACGAACATGAAAGCTCTCAACGGCATTGCCGCCTTAc                                                                                                    <  5:204814/143‑1 (MQ=255)
                                                    gCCAGGTGTCAGCTTTGCACCGTCTGCGACTGACAAAGATGTAATCCACAGATCACCGGGGAGACAGGTGCTGCCCCCTAAGATTCAGTCACACCAAATCGGAGTGACGAACATGAAAGCTCTCAACGGCATTGCCGCCTTAc                                                                                                    >  6:204814/1‑143 (MQ=255)
                                                    gCCAGGTGTCAGCTTTGCACCGTCTGCGACTGACAAAGATGTAATCCACAGATCACCGGGGAGACAGGTGCTGCCCCCTAAGATTCAGTCACACCAAATCGGAGTGACGAACATGAAAGCTCTCAACGGCATTGCCGCCTTAc                                                                                                    >  8:21314/1‑143 (MQ=255)
                                                       aGGTGTCAGCTTTGCACCGTCTGCGACTGACAAAGATGTAATCCACAGATCACCGGGGAGACAGGTGCTGCCCCCTAAGATTCAGTCACACCAAATCGGAGTGACGAACATGAAAGCTCTCAACGGCATTGCCGCCTTACTGATTCTGa                                                                                           >  2:13881/1‑149 (MQ=255)
                                                        ggTGTCAGCTTTGCACCGTCTGCGACTGACAAAGATGTAATCCACAGATCACCGGGGAGACAGGTGCTGCCCCCTAAGATTCAGTCACACCAAATCGGAGTGACGAACATGAAAGCTCTCAACGGCATTGCCGCCTTACTGATTCTGAt                                                                                          <  7:77934/149‑1 (MQ=255)
                                                              aGCTTTGCACCGTCTGCGACTGACAAAGATGTAATCCACAGATCACCGGGGAGACAGGTGCTGCCCCCTAAGATTCAGTCACACCAAATCGGAGTGACGAACATGAAAGCTCTCAACGGCATTGCCGCCTTACTGATTCTGATgg                                                                                        >  4:337004/1‑145 (MQ=255)
                                                              aGCTTTGCACCGTCTGCGACTGACAAAGATGTAATCCACAGATCACCGGGGAGACAGGTGCTGCCCCCTAAGATTCAGTCACACCAAATCGGAGTGACGAACATGAAAGCTCTCAACGGCATTGCCGCCTTACTGATTCTGATgg                                                                                        <  3:337004/145‑1 (MQ=255)
                                                                cTTTGCACCGTCTGCGACTGACAAAGATGTAATCCACAGATCACCGGGGAGACAGGTGCTGCCCCCTAAGATTCAGTCACACCAAATCGGAGTGACGAACATGAAAGCTCTCAACGGCATTGCCGCCTTAc                                                                                                    <  8:195213/131‑1 (MQ=255)
                                                                cTTTGCACCGTCTGCGACTGACAAAGATGTAATCCACAGATCACCGGGGAGACAGGTGCTGCCCCCTAAGATTCAGTCACACCAAATCGGAGTGACGAACATGAAAGCTCTCAACGGCATTGCCGCCTTACTGATTCTGATGGCTTCCt                                                                                  >  2:294196/1‑149 (MQ=255)
                                                                        cGTCTGCGACTGACAAAGATGTAATCCACAGATCACCGGGGAGACAGGTGCTGCCCCCTAAGATTAAGTCACACCAAATCGGAGTGACGAACATGAAAGCTCTCAACGGCATTGCCGCCTTACTGATTCTGATGGCTTCCTCGGCcgc                                                                           >  4:171945/1‑148 (MQ=255)
                                                                                    aCAAAGATGTAATCCACAGATCACCGGGGAGACAGGTGCTGCCCCCTAAGATTCAGTCACACCAAATCGGAGTGACGAACATGAAAGCTCTCAACGGCATTGCCGCCTTACTGATTCTGATGGCt                                                                                      >  7:218803/1‑125 (MQ=255)
                                                                                    aCAAAGATGTAATCCACAGATCACCGGGGAGACAGGTGCTGCCCCCTAAGATTCAGTCACACCAAATCGGAGTGACGAACATGAAAGCTCTCAACGGCATTGCCGCCTTACTGATTCTGATGGCt                                                                                      <  8:218803/125‑1 (MQ=255)
                                                                                    aCAAAGATGTAATCCACAGATCACCGGGGAGACAGGTGCTGCCCCCTAAGATTCAGTCACACCAAATCGGAGTGACGAACATGAAAGCTCTCAACGGCATTGCCGCCTTACTGATTCTGATGGCTTCCTCGGCCGCGATGGCTGAAGGt                                                              <  4:60487/149‑1 (MQ=255)
                                                                                    aCAAAGATGTAATCCACAGATCACCGGGGAGACAGGTGCTGCCCCCTAAGATTCAGTCACACCAAATCGGAGTGACGAACATGAAAGCTCTCAACGGCATTGCCGCCTTACTGATTCTGATGGCTTCCTCGGCCGCGATGGCTGAAGGt                                                              <  1:13881/149‑1 (MQ=255)
                                                                                     cAAAGATGTAATCCACAGATCACCGGGGAGACAGGTGCTGCCCCCTAAGATTCAGTCACACCAAATCGGAGTGACGAACATGAAAGCTCTCAACGGCATTGCCGCCTTACTGATTCTGATGGCTTCCTCGGCCGCGATGGCTGAAGGTg                                                             <  2:62887/149‑1 (MQ=255)
                                                                                      aaaGATGTAATCCACAGATCACCGGGGAGACAGGTGCTGCCCCCTAAGATTCAGTCACACCAAATCGGAGTGACGAACATGAAAGCTCTCGACGGCATTGCCGCCTTACTGATTCTGATGGCTTCCTCGGCCGCGATGGCTGAAGGTgg                                                            <  7:151182/149‑1 (MQ=255)
                                                                                        aGATGTAATCCACAGATCACCGGGGAGACAGGTGCTGCCCCCTAAGATTCAGTCACACCAAATCGGAGTGACGAACATGAAAGCTCTCAACGGCATTGCCGCCTTACTGATTCTGATGGCTTCCTCGGCCGCGATGGCTGAAGGTGGa                                                           >  8:277093/1‑148 (MQ=255)
                                                                                            gTAATCCACAGATCACCGGGGAGACAGGTGCTGCCCCCTAAGATTCAGTCACACCAAATCGGAGTGACGAACATGAAAGCTCTCAACGGCATTGCCGCCTTACTGATTCTGATGGCt                                                                                      >  4:195972/1‑117 (MQ=255)
                                                                                            gTAATCCACAGATCACCGGGGAGACAGGTGCTGCCCCCTAAGATTCAGTCACACCAAATCGGAGTGACGAACATGAAAGCTCTCAACGGCATTGCCGCCTTACTGATTCTGATGGCt                                                                                      <  3:195972/117‑1 (MQ=255)
                                                                                                tCCACAGATCACCGGGGAGACAGGTGCTGCCCCCTAAGATTCAGTCACACCAAATCGGAGTGACGAACATGAAAGCTCTCAACGGCATTGCCGCCTTACTGATTCTGATGGCTTCCTCGGCCGCGATGGCTGAAGGTGGATCttcccgt                                                  >  1:68576/1‑143 (MQ=255)
                                                                                                   acaGATCACCGGGGAGACAGGTGCTGCCCCCTAAGATTCAGTCACACCAAATCGGAGTGACGAACATGAAAGCTCTCAACGGCATTGCCGCCTTACt                                                                                                   <  2:165555/97‑1 (MQ=255)
                                                                                                   acaGATCACCGGGGAGACAGGTGCTGCCCCCTAAGATTCAGTCACACCAAATCGGAGTGACGAACATGAAAGCTCTCAACGGCATTGCCGCCTTACt                                                                                                   <  8:125981/97‑1 (MQ=255)
                                                                                                   acaGATCACCGGGGAGACAGGTGCTGCCCCCTAAGATTCAGTCACACCAAATCGGAGTGACGAACATGAAAGCTCTCAACGGCATTGCCGCCTTACt                                                                                                   >  1:165555/1‑97 (MQ=255)
                                                                                                   acaGATCACCGGGGAGACAGGTGCTGCCCCCTAAGATTCAGTCACACCAAATCGGAGTGACGAACATGAAAGCTCTCAACGGCATTGCCGCCTTACt                                                                                                   >  7:125981/1‑97 (MQ=255)
                                                                                                                  gaCAGGTGCTGCCCCCTAAGATTCAGTCACACCAAATCGGAGTGACGAACATGAAAGCTCTCAACGGCATTGCCGCCTTACTGATTCTGATGGCTTCCTCGGCCGCGATGGCTGAAGGTGGATCTGACCGCCTGCACGGGAAGATGAtt                                <  1:294196/149‑1 (MQ=255)
                                                                                                                     aGGTGCTGCCCCCTAAGATTCAGTCACACCAAATCGGAGTGACGAACATGAAAGCTCTCAACGGCATTGCCGCCTTACTGATTCTGATGGCTTCCTCGGCCGCGATGGCTGAAGGTGGATCTGACCGCCTGCACGGGAAgatga                                  >  2:302139/1‑144 (MQ=255)
                                                                                                                     aGGTGCTGCCCCCTAAGATTCAGTCACACCAAATCGGAGTGACGAACATGAAAGCTCTCAACGGCATTGCCGCCTTACTGATTCTGATGGCTTCCTCGGCCGCGATGGCTGAAGGTGGATCTGACCGCCTGCACGGGAAgatga                                  <  1:302139/144‑1 (MQ=255)
                                                                                                                      ggTGCTGCCCCCTAAGATTCAGTCACACCAAATCGGAGTGACGAACATGAAAGCTCTCAACGGCATTGCCGCCTTACTGATTCTGATGGCTTCCTCGGCCGCGATGGCTGAAGGTGGATCTGACCGCCTGCACGGGAAGATGATTCAgg                            <  7:277093/149‑1 (MQ=255)
                                                                                                                                  tAAGATTCAGTCACACCAAATCGGAGTGACGAACATGAAAGCTCTCAACGGCATTGCCGCCTTACTGATTCTGATGGCTTCCTCGGCCGCGATGGCTGAAGGTGGATCTGACCGCCTGCACGGGAAGATGATTCAGGCGAATGaa                    <  6:14238/145‑1 (MQ=255)
                                                                                                                                  tAAGATTCAGTCACACCAAATCGGAGTGACGAACATGAAAGCTCTCAACGGCATTGCCGCCTTACTGATTCTGATGGCTTCCTCGGCCGCGATGGCTGAAGGTGGATCTGACCGCCTGCACGGGAAGATGATTCAGGCGAATGaa                    >  5:14238/1‑145 (MQ=255)
                                                                                                                                  tAAGATTCAGTCACACCAAATCGGAGTGACGAACATGAAAGCTCTCAACGGCATTGCCGCCTTACTGATTCTGATGGCTTCCTCGGCCGCGATGGCTGAAGGTGGATCTGACCGCCTGCACGGGAAGATGATTCAGGCGAATGAACAgg                >  7:62909/1‑149 (MQ=255)
                                                                                                                                      tgttcgTCACTCCGATTTGGTGTGACGAACATGAAAGCTCTCAACGGCATTGCCGCCTTACTGATTCTGATGGCTTCCTCGGCCGCGATGGCTGAAGGTGGATCTGACCGCCTGCACGGGAAGATGATTCa                              <  6:70807/126‑1 (MQ=255)
                                                                                                                                      tgttcgTCACTCCGATTTGGTGTGACGAACATGAAAGCTCTCAACGGCATTGCCGCCTTACTGATTCTGATGGCTTCCTCGGCCGCGATGGCTGAAGGTGGATCTGACCGCCTGCACGGGAAGATGATTCa                              >  5:70807/6‑131 (MQ=255)
                                                                                                                                      aTTCAGTCACACCAAATCGGAGTGACGAACATGAAAGCTCTCAACGGCATTGCCGCCTTACTGATTCTGATGGCTTCCTCGGCCGCGATGGCTGAAGGTGGATCTGACCGCCTGCACGGGAAGATGATTCAGGCGAATGAACAGGCCAt            >  8:78354/1‑149 (MQ=255)
                                                                                                                                        ttcgTCACTCCGATTTGGTGTGACGAACATGAAAGCTCTCAACGGCATTGCCGCCTTACTGATTCTGATGGCTTCCTCGGCCGCGATGGCTGAAGGTGGATCTGACCGCCTGCACGGGAAga                                     <  6:126099/119‑1 (MQ=255)
                                                                                                                                        ttcgTCACTCCGATTTGGTGTGACGAACATGAAAGCTCTCAACGGCATTGCCGCCTTACTGATTCTGATGGCTTCCTCGGCCGCGATGGCTGAAGGTGGATCTGACCGCCTGCACGGGAAga                                     >  5:126099/4‑122 (MQ=255)
                                                                                                                                                acCAAATCGGAGTGACGAACATGAAAGCTCTCAACGGCATTGCCGCCTTACTGATTCTGATGGCTTc                                                                                    >  4:71155/1‑67 (MQ=255)
                                                                                                                                                acCAAATCGGAGTGACGAACATGAAAGCTCTCAACGGCATTGCCGCCTTACTGATTCTGATGGCTTCCTCGGCCGCGATGGCTGAAGGTGGATCTGACCGCATGCACGGGAAGATGATTCAGGCGAATGAACAGGCCATGCGCGCATAc  >  7:316585/1‑149 (MQ=255)
                                                                                                                                                   |                                                                                                                                                 
TCTCAGCAACAATCCTTCTAGCAGTATAGGCTGCCCATTAGGCATCACTCTTGCCAGGTGTCAGCTTTGCACCGTCTGCGACTGACAAAGATGTAATCCACAGATCACCGGGGAGACAGGTGCTGCCCCCTAAGATTCAGTCACACCAAATCGGAGTGACGAACATGAAAGCTCTCAACGGCATTGCCGCCTTACTGATTCTGATGGCTTCCTCGGCCGCGATGGCTGAAGGTGGATCTGACCGCCTGCACGGGAAGATGATTCAGGCGAATGAACAGGCCATGCGCGCATAC  >  NC_002947/6128642‑6128934

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑