Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 558,477 A→C 6.9% E21D (GAA→GAC)  rplR → 50S ribosomal protein L18

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_002947558,4770AC 6.9% 177.3 / 4.5 58E21D (GAA→GAC) rplR50S ribosomal protein L18
Reads supporting (aligned to +/- strand):  ref base A (28/26);  new base C (2/2);  total (30/28)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.13e-01

CAGAGCCTTACAAAGGTAAAGGTGTGCGTTACGCGGACGAAGTAGTCCGTCGTAAAGAAGCCAAGAAGAAGTAGGGCCTAGTAAATGACCGACAAAAAAGTTATTCGACTGCGTCGCGCTCGCAAAGCACGTCTCAAGATGCACGAACTCGAAGTCGTGCGCCTGTGCGTGTTCCGCTCCTCGCAGCACATCTACGCCCAGGTCATTTCGGCCGACGGCAGCAAGGTTCTGGCAAGCGCCTCGACCTTGGACAAAGACCTGCGTGATGGCGCCACTGGCAACATCGACGCGGC  >  NC_002947/558331‑558623
                                                                                                                                                  |                                                                                                                                                  
cAGAGCCTTACAAAGGTAAAGGTGTGCGTTACGCGGACGAAGTAGTCCGTCGTAAAGAAGCCAAGAAGAAGTAGGGCCTAGTAAATGACCGACAAAAAAGTTATTCGACTGCGTCGCGCTCGCAAAGCACGTCTCAAGATGCACGAACt                                                                                                                                                  >  5:64890/1‑149 (MQ=255)
    gCCTTACAAAGGTAAAGGTGTGCGTTACGCGGACGAAGTAGTCCGTCGTAAAGAAGCCAAGAAGAAGTAGGGCCTAGTAAATGACCGACAAAAAAGTTATTCGACTGCGTCGCGCTCGCAAAGCACGTCTCAAGATGCACGAACTCGaa                                                                                                                                              <  3:191513/149‑1 (MQ=255)
      cTTACAAAGGTAAAGGTGTGCGTTACGCGGACGAAGTAGTCCGTCGTAAAGAAGCCAAGAAGAAGTAGGGCCTAGTAAATGACCGACAAAAAAGTTATTCGACTGCGTCGCGCTCGCAAAGCACGTCTCAAGATGCACGAACTCGAAGt                                                                                                                                            >  6:157823/1‑149 (MQ=255)
       ttACAAAGGTAAAGGTGTGCGTTACGCGGACGAAGTAGTCCGTCGTAAAGAAGCCAAGAAGAAGTAGGGCCTAGTAAATGACCGACAAAAAAGTTATTCGACTGCGTCGCGCTCGCAAAGCACGTCTCAAGATGCACGACTTCGagttc                                                                                                                                           >  1:310794/1‑145 (MQ=255)
       ttACAAAGGTAAAGGTGTGCGTTACGCGGACGAAGTAGTCCGTCGTAAAGAAGCCAAGAAGAAGTAGGGCCTAGTAAATGACCGACAAAAAAGTTATTCGACTGCGTCGCGCTCGCAAAGCACGTCTCAAGATGCACGAACTCGAAg                                                                                                                                             <  8:287363/147‑1 (MQ=255)
       ttACAAAGGTAAAGGTGTGCGTTACGCGGACGAAGTAGTCCGTCGTAAAGAAGCCAAGAAGAAGTAGGGCCTAGTAAATGACCGACAAAAAAGTTATTCGACTGCGTCGCGCTCGCAAAGCACGTCTCAAGATGCACGAACTCGAAGta                                                                                                                                           >  7:287363/1‑148 (MQ=255)
          cAAAGGTAAAGGTGTGCGTTACGCGGACGAAGTAGTCCGTCGTAAAGAAGCCAAGAAGAAGTAGGGCCTAGTAAATGACCGACAAAAAAGTTATTCGACTGCGTCGCGCTCGCAAAGCACGTCTCAAGATGCACGAACTCGAAGTCGt                                                                                                                                         <  3:248738/148‑1 (MQ=255)
            aaggtaaaggtGTGCGTTACGCGGACGAAGTAGTCCGTCGTAAAGAAGCCAAGAAGAAGTAGGGCCTAGTAAATGACCGACAAAAAAGTTATTCGACTGCGTCGCGCTCGCAAAGCACGTCTCAAGATGCACGACTTCGAGTTCGTgca                                                                                                                                      <  2:310794/149‑2 (MQ=255)
             aggtaaaggtGTGCGTTACGCGGACGAAGTAGTCCGTCGTAAAGAAGCCAAGAAGAAGTAGGGCCTAGTAAATGACCGACAAAAAAGTTATTCGACTGCGTCGCGCTCGCAAAGCACGTCTCAAGATGCACGAACTCGAAGTCGTgcgc                                                                                                                                     <  6:64890/149‑1 (MQ=255)
             aggtaaaggtGTGCGTTACGCGGACGAAGTAGTCCGTCGTAAAGAAGCCAAGAAGAAGTAGGGCCTAGTAAATGACCGACAAAAAAGTTATTCGACTGCGTCGCGCTCGCAAAGCACGTCTCAAGATGCACGAACTCGAAGTCGTgcgc                                                                                                                                     <  4:139864/149‑1 (MQ=255)
                 aaaggtGTGCGTTACGCGGACGAAGTAGTCCGTCGTAAAGAAGCCAAGAAGAAGTAGGGCCTAGTAAATGACCGACAAAAAAGTTATTCGACTGCGTCGCGCTCGCAAAGCACGTCTCAAGACGCACGAACTCGAAGTCGTGCGCCtgt                                                                                                                                 >  3:257748/1‑149 (MQ=255)
                 aaacgtGTGCGTTACGCGGACGAAGTAGTCCGTCGTAAAGAAGCCAAGAAGAAGTAGGGCCTAGTAAATGACCGACAAAAAAGTTATTCGACTGCGTCGCGCTCGCAAAGCACGGCTCAAGATGCACGAACTCGAAGTCGTGCGCCtgt                                                                                                                                 >  3:257752/1‑149 (MQ=255)
                   aggtGTGCGTTACGCGGACGAAGTAGTCCGTCGTAAAGAAGCCAAGAAGAAGTAGGGCCTAGTAAATGACCGACAAAAAAGTTATTCGACTGCGTCGCGCTCGCAAAGCACGTCTCAAGATGCACGAACTCGAAGTCGTGCGCCTGTGc                                                                                                                               <  4:261057/149‑1 (MQ=255)
                                 cgGACGAAGTAGTCCGTCGTAAAGAAGCCAAGAAGAAGTAGGGCCTAGTAAATGACCGACAAAAAAGTTATTCGACTGCGTCGCGCTCGCAAAGCACGTCTCAAGATGCACGAACTCGAAGTCGTGCGCCTGTGCGTGTTCCGctcctc                                                                                                                 <  6:333038/149‑1 (MQ=255)
                                      gAAGTAGTCCGTCGTAAAGAAGCCAAGAAGAAGTAGGGCCTAGTAAATGACCGACAAAAAAGTTATTCGACTGCGTCGCGCTCGCAAAGCACGTCTCAAGATGCACGAACTCGAAGTCGTGCGCCTGTGCGTGTTCCGCTCCTCgcag                                                                                                             >  5:127863/1‑148 (MQ=255)
                                                 tcgtAAAGAAGCCAAGAAGAAGTAGGGCCTAGTAAATGACCGACAAAAAAGTTATTCGACTGCGTCGCGCTCGCAAAGCACGTCTCAAGATGCACGACTTCGAGTTCGTgcatc                                                                                                                                    <  6:89457/114‑4 (MQ=255)
                                                    tAAAGAAGCCAAGAAGAAGTAGGGCCTAGTAAATGACCGACAAAAAAGTTATTCGACTGCGTCGCGCTCGCAAAGCACGTCTCAAGATGCACGAACTCGAAGTCGTGCGCCTGTGc                                                                                                                               <  2:320776/116‑1 (MQ=255)
                                                    tAAAGAAGCCAAGAAGAAGTAGGGCCTAGTAAATGACCGACAAAAAAGTTATTCGACTGCGTCGCGCTCGCAAAGCACGTCTCAAGATGCACGAACTCGAAGTCGTGCGCCTGTGc                                                                                                                               >  1:320776/1‑116 (MQ=255)
                                                    tAAAGAAGCCAAGAAGAAGTAGGGCCTAGTAAATGACCGACAAAAAAGTTATTCGACTGCGTCGCGCTCGCAAAGCACGTCTCAAGATGCACGAACTCGAAGTCGTGCGCCTGTGCGTGTTc                                                                                                                         >  5:69041/1‑122 (MQ=255)
                                                    tAAAGAAGCCAAGAAGAAGTAGGGCCTAGTAAATGACCGACAAAAAAGTTATTCGACTGCGTCGCGCTCGCAAAGCACGTCTCAAGATGCACGAACTCGAAGTCGTGCGCCTGTGCGTGTTc                                                                                                                         <  6:69041/122‑1 (MQ=255)
                                                       agaagCCAAGAAGAAGTAGGGCCTAGTAAATGACCGACAAAAAAGTTATTCGACTGCGTCGCGCTCGCAAAGCACGTCTCAAGATGCACGAACTCGAAGTCGTGCGCCTGTGCGTGTTCCGCTCCTCGCAGCACATCTACGCCCAGGt                                                                                            >  1:50353/1‑148 (MQ=255)
                                                              acgaagaagTCGGGCCTAGTACATGCCCGACAAAAAAGTTATGCGACTGCGGCGCGCTCGCAACGCACGTCGCAAGATGCACGAACTCGAAGTCGTGCGCCTGTGCGTGTTCCGCTCCTCGCAGCACATCTACGCCCAGGTCATTTCgg                                                                                    <  4:257752/147‑1 (MQ=255)
                                                              aagaagaagTAGGGCCTAGTAAATGACCGACAAAAAAGTTATTCGACTGCGTCGCGCTCGCAAAGCACGTCTCAAGACGCACGAACTCGAAGTCGTGCGCCTGTGCGTGTTCCGCTCCTCGCAGCACATCTACGCCCAGGTCATTTCgg                                                                                    <  4:257748/149‑1 (MQ=255)
                                                                       tAGGGCCTAGTAAATGACCGACAAAAAAGTTATTCGACTGCGTCGCGCTCGCAAAGCACGTCTCAAGATGCACGAACTCGAAGTCGTGCGCCTGTGCGTGTTCCGCTCCTCgcagca                                                                                                           <  8:333410/117‑1 (MQ=255)
                                                                       tAGGGCCTAGTAAATGACCGACAAAAAAGTTATTCGACTGCGTCGCGCTCGCAAAGCACGTCTCAAGATGCACGAACTCGAAGTCGTGCGCCTGTGCGTGTTCCGCTCCTCgcagca                                                                                                           >  7:333410/1‑117 (MQ=255)
                                                                        aGGGCCTAGTAAATGACCGACAAAAAAGTTATTCGACTGCGTCGCGCTCGCAAAGCACGTCTCAAGATGCACGAACTCGAAGTCGTGCGCCTGTGCGTGTTCCGCTCCTCGCAGCACATCTACGCCCAGGTCATTTCGGCCGACGgcag                                                                          >  7:137167/1‑149 (MQ=255)
                                                                               aGTAAATGACCGACAAAAAAGTTATTCGACTGCGTCGCGCTCGCAAAGCACGTCTCAAGATGCACGAACTCGAAGTCGTGCGCCTGTGCGTGTTCCGCTCCTCGCAGCACATCTACGCCCAg                                                                                              <  5:166938/122‑1 (MQ=255)
                                                                               aGTAAATGACCGACAAAAAAGTTATTCGACTGCGTCGCGCTCGCAAAGCACGTCTCAAGATGCACGAACTCGAAGTCGTGCGCCTGTGCGTGTTCCGCTCCTCGCAGCACATCTACGCCCAg                                                                                              >  6:166938/1‑122 (MQ=255)
                                                                                gTAAATGACCGACAAAAAAGTTATTCGACTGCGTCGCGCTCGCAATGCACGTCTCAAGATGCACGAACTCGAAGTCGTGCGCCTGTGCGTGTTCCGCTCCTCGCAGCACATCt                                                                                                      >  1:244842/1‑113 (MQ=255)
                                                                                gTAAATGACCGACAAAAAAGTTATTCGACTGCGTCGCGCTCGCAAAGCACGTCTCAAGATGCACGAACTCGAAGTCGTGCGCCTGTGCGTGTTCCGCTCCTCGCAGCACATCt                                                                                                      <  2:244842/113‑1 (MQ=255)
                                                                                  aaaTGACCGACAAAAAAGTTATTCGACTGCGTCGCGCTCGCAAAGCACGTCTCAAGATGCACGAACTCGAAGTCGTGCGCCTGTGCGTGTTCCGCTCCTCGCAGCACATCTACGCCCAGGTCATTTCGGCCGACGGCAGCAAGGTTCt                                                                 <  2:50353/148‑1 (MQ=255)
                                                                                   aaTGACCGACAAAAAAGTTATTCGACTGCGTCGCGCTCGCAAAGCACGTCTCAAGATGCACGAACTCGAAGTCGTGCGCCTGTGCGTGTTCCGCTCCTCGCAGCACATCTACGCCCAGGTCATTTCGGCCGACGGCAGCAAGGTTCTg                                                                <  6:127863/148‑1 (MQ=255)
                                                                                      gACCGACAAAAAAGTTATTCGACTGCGTCGCGCTCGCAAAGCACGTCTCAAGATGCACGAACTCGAAGTCGTGCGCCTGTGCATGTTCCGCTCCTCGCAGCACATCTACGCCCAGGTCATTTCGGCCGACGGCAGCAAGGTTCTGGc                                                              >  5:28446/1‑147 (MQ=255)
                                                                                                aaaGTTATTCGACTGCGTCGCGCTCGCAAAGCACGTCTCAAGATGCACGAACTCGAAGTCGTGCGCCTGTGCGTGTTCCGctcct                                                                                                                  <  5:184425/85‑1 (MQ=255)
                                                                                                aaaGTTATTCGACTGCGTCGCGCTCGCAAAGCACGTCTCAAGATGCACGAACTCGAAGTCGTGCGCCTGTGCGTGTTCCGctcct                                                                                                                  >  6:184425/1‑85 (MQ=255)
                                                                                                     tATTCGACTGCGTCGCGCTCGCAAAGCACGTCTCAAGATGCACGAACTCGAAGTCGTGCGCCTGTGCGTGTTCCGCTCCTCGCAGCACATCTACGCCCAGGTCATTTCGGCCGACGGCAGCAAGGTTCTGGCAAGCGCCTCGACCTTg                                              <  8:137167/148‑1 (MQ=255)
                                                                                                      aTTCGACTGCGTCGCGCTCGCAAAGCACGTCTCAAGATGCACGAACTCGAAGTCGTGCGCCTGTGCGTGTTCCGCTCCTCGCAGCACATCTACGCCCAGGTCAttt                                                                                       >  6:319544/1‑106 (MQ=255)
                                                                                                      aTTCGACTGCGTCGCGCTCGCAAAGCACGTCTCAAGATGCACGAACTCGAAGTCGTGCGCCTGTGCGTGTTCCGCTCCTCGCAGCACATCTACGCCCAGGTCAttt                                                                                       <  5:319544/106‑1 (MQ=255)
                                                                                                       ttCGACTGCGTCGCGCTCGCAAAGCACGTCTCAAGATGCACGAACTCGAAGTCGTGCGCCTGTGCGTGTTCCGCTCCTCGCAGCACATCTACGCCCAGGTCATTTCGGCCGACGGCAGCAAGGTTCTGGCAAGCGCCTCGACCTTGGAc                                           <  5:157823/149‑1 (MQ=255)
                                                                                                          gACTGCGTCGCGCTCGCAAAGCACGTCTCAAGATGCACGAACTCGAAGTCGTGCGCCTGTGCGTGTTCCGCTCCTCGCAGCACATCTACGCCCAGGTCa                                                                                          >  5:237466/1‑99 (MQ=255)
                                                                                                          gACTGCGTCGCGCTCGCAAAGCACGTCTCAAGATGCACGAACTCGAAGTCGTGCGCCTGTGCGTGTTCCGCTCCTCGCAGCACATCTACGCCCAGGTCa                                                                                          <  6:237466/99‑1 (MQ=255)
                                                                                                              gCGTCGCGCTCGCAAAGCACGTCTCAAGATGCACGAACTCGAAGTCGTGCGCCTGTGCGTGTTCCGCTCCTCGCAGCACATCTACGCCCAGGTCATTTCGGCCGACGGCAGCAAGGTTCTGGCAAGCGCCTCGACCTTGGACAAAGAcc                                    >  6:289183/1‑149 (MQ=255)
                                                                                                                    cgcTCGCAAAGCACGTCTCAAGATGCACGAACTCGAAGTCGTGCGCCTGTGCGTGTTCCGCTCCTCGCAGCACATCTACGCCCAGGTCATTTCGGCCGACGGCAGCAAGGTTCTGGCAAGCGCCTCGACCTTGGACAAAGACCTGCGt                               >  2:305228/1‑148 (MQ=255)
                                                                                                                       tCGCAAAGCACGTCTCAAGATGCACGAACTCGAAGTCGTGCGCCTGTGCGTGTTCCGCTCCTCGCAGCACATCTACGCCCAGGTCATTTCGGCCGACGGCAGCAAGGTTCTGGCAAGCGCCTCGACCTTGGAc                                           <  3:204440/133‑1 (MQ=255)
                                                                                                                       tCGCAAAGCACGTCTCAAGATGCACGAACTCGAAGTCGTGCGCCTGTGCGTGTTCCGCTCCTCGCAGCACATCTACGCCCAGGTCATTTCGGCCGACGGCAGCAAGGTTCTGGCAAGCGCCTCGACCTTGGAc                                           >  4:204440/1‑133 (MQ=255)
                                                                                                                              gCACGTCTCAAGATGCACGAACTCGAAGTCGTGCGCCTGTGCGTGTTCCGCTCCTCGCAGCACATCTACGCCCAGGTCATTTCGGCCGACGGCAGCAAGGTTCTGGCAAGCGCCTCGACCTTGGACAAAGACCTGCGTGATGGCGCCAc                    >  8:93785/1‑149 (MQ=255)
                                                                                                                               cACGTCTCAAGATGCACGAACTCGAAGTCGTGCGCCTGTGCATGTTCCGCTCCTCGCAGCACATCTACGCCCAGGTCATTTCGCCCGACGGCAGCAAGGTTCTGGCAAGCGCCTCGACCTTGGACAAAGACCTGCGTGATGGCGCCACt                   <  6:28446/149‑1 (MQ=255)
                                                                                                                                aCGTCTCAAGATGCACGAACTCGAAGTCGTGCGCCTGTGCGTGTTCCGCTCCTCGCAGCACATCTACGCCCAGGTCATTTCGGCCGACGGCAGCAAGGTTCTGGCAAGCGCCTCGACCTTGGACAAAGACCTGCGTGATGGCGCCACTg                  >  4:22428/1‑149 (MQ=255)
                                                                                                                                aCGTCTCAAGATGCACGAACTCGAAGTCGTGCGCCTGTGCGTGTTCCGCTCCTCGCAGCACATCTACGCCCAGGTCATTTCGGCCGACGGCAGCAAGGTTCTGGCAAGCGCCTCGACCTTGGACAAAGACCTGCGTGATGGCGCCACTg                  >  4:187099/1‑149 (MQ=255)
                                                                                                                                   tctcAAGATGCACGAACTCGAAGTCGTGCGCCTGTGCGTGTTCCGCTCCTCGCAGCACATCTACGCCCAGGTCATTTCGGCCGACGGCAGCAAGGTTCTGGCAAGCGCCTCGACCTTGGACAAAGACCTGCGTGATGGCGCCACTGGc                >  4:86443/1‑148 (MQ=255)
                                                                                                                                   tctcAAGATGCACGAACTCGAAGTCGTGCGCCTGTGCGTGTTCCGCTCCTCGCAGCACATCTACGCCCAGGTCATTTCGGCCGACGGCAGCAAGGTTCTGGCAAGCGCCTCGACCTTGGACAAAGACCTGCGTGATGGCGCCACTGGCa               <  3:86443/149‑1 (MQ=255)
                                                                                                                                     tcAAGATGCACGAACTCGAAGTCGTGCGCCTGTGCGTGTTCCGCTCCTCGCAGCACATCTACGCCCAGGTCATTTCGGCCGACGGCAGCAAGGTTCTGGCAAGCGCCTCGACCTTGGACAAAGACCTGCGTGATGGCGCCACTGGCaa              >  5:56679/1‑148 (MQ=255)
                                                                                                                                         ggcgCACGACTTCGAGTTCGTGCGCCTGTGCGTGTTCCGCTCCTCGCAGCACATCTACGCCCAGGTCATTTCGGCCGACGGCAGCAAGGTTCTGGCAAGCGCCTCGACCTTGGACAAAGACCTGCGTGATGGCGCCACTGGCAACATCg         >  4:290369/4‑149 (MQ=255)
                                                                                                                                          aTGCACGAACTCGAAGTCGTGCGCCTGTGCGTGTTCCGCTCCTCGCAGCACATCTACGCCCAGGTCATTTCGGCCGACGGCAGCAAGGTTCTGGCAAGCGCCTCGACCTTGGACAAAGACCTGCGTGATGGCGCCACTGGCAACATCGa        <  3:22428/149‑1 (MQ=255)
                                                                                                                                            gCACGAACTCGAAGTCGTGCGCCTGTGCGTGTTCCGCTCCTCGCAGCACATCTACGCCCAGGTCATTTCGGCCGACGGCAGCAAGGTTCTGGCAAGCGCCTCGACCTTGGACAAAGACCTg                                  >  2:105942/1‑121 (MQ=255)
                                                                                                                                            gCACGAACTCGAAGTCGTGCGCCTGTGCGTGTTCCGCTCCTCGCAGCACATCTACGCCCAGGTCATTTCGGCCGACGGCAGCAAGGTTCTGGCAAGCGCCTCGACCTTGGACAAAGACCTg                                  <  1:105942/121‑1 (MQ=255)
                                                                                                                                              aCGAACTCGAAGTCGTGCGCCTGTGCGTGTTCCGCTCCTCGCAGCACATCTACGCCCAGGTCATTTCGGCCGACGGCAGCAAGGTTCTGGCAAGCGCCTCGACCTTGGACAAAGACCTGCGTGATGTTGCCAGTGGCGCCATcacgcag    >  5:154222/1‑143 (MQ=255)
                                                                                                                                                gAACTCGAAGTCGTGCGCCTGTGCGTGTTCCGCTCCTCGCAGCACATCTACGCCCAGGTCATTTCGGCCGACGGCAGCAAGGTTCTGGCAAGCGCCGCGACCGTGGGCAAAGACCTGCGTGATGGCGCCACTGGCAACCTCGACGCGGc  >  4:331895/1‑149 (MQ=255)
                                                                                                                                                  |                                                                                                                                                  
CAGAGCCTTACAAAGGTAAAGGTGTGCGTTACGCGGACGAAGTAGTCCGTCGTAAAGAAGCCAAGAAGAAGTAGGGCCTAGTAAATGACCGACAAAAAAGTTATTCGACTGCGTCGCGCTCGCAAAGCACGTCTCAAGATGCACGAACTCGAAGTCGTGCGCCTGTGCGTGTTCCGCTCCTCGCAGCACATCTACGCCCAGGTCATTTCGGCCGACGGCAGCAAGGTTCTGGCAAGCGCCTCGACCTTGGACAAAGACCTGCGTGATGGCGCCACTGGCAACATCGACGCGGC  >  NC_002947/558331‑558623

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑