Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 561,964 T→C 6.0% V32A (GTG→GCG)  rpsK → 30S ribosomal protein S11

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_002947561,9640TC 6.0% 191.5 / 2.2 66V32A (GTG→GCG) rpsK30S ribosomal protein S11
Reads supporting (aligned to +/- strand):  ref base T (31/31);  new base C (2/2);  total (33/33)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.82e-01

TAAGGGCCCGCGTAAGCCGATCCGCAAGTAATCGCCCAGGAATATAGACATGGCAAAACCTGCTGCTCGTCCTCGTAAGAAAGTCAAAAAGACAGTGGTTGATGGCATCGCCCACATCCATGCTTCTTTCAACAACACCATCGTGACCATCACCGACCGTCAGGGCAATGCTTTGTCCTGGGCGACCTCCGGTGGTTCGGGTTTCCGTGGTTCGCGCAAATCCACCCCGTTCGCAGCTCAGATCGCTGCTGAGCGTGCTGGTCAAGCTGCGCTGGAATACGGTCTGAAGAAC  >  NC_002947/561821‑562112
                                                                                                                                               |                                                                                                                                                    
tAAGGGCCCGCGTAAGCCGATCCGCAAGTAATCGCCCAGGAATATAGACATGGCAAAACCTGCTGCTCGTCCTCGTAAGAAAGTCAAAAAGACAGTGGTTGATGGCATCGCCCACATCCATGCTTCTTTCAACAACACCATCGTGACCa                                                                                                                                                 >  3:302093/1‑149 (MQ=255)
    ggCCCGCGTAAGCCGATCCGCAAGTAATCGCCCAGGAATATAGACATGGCAAAACCTGCTGCTCGTCCTCGTAAGAAAGTCAAAAAGACAGTGGTTGATGGCATCGCCCACATCCATGCTTCTTTCAACAACACCATCGCGACCATCac                                                                                                                                             >  3:171973/1‑149 (MQ=255)
            tAAGCCGATCCGCAAGTAATCGCCCAGGAATATAGACATGGCAAAACCTGCTGCTCGTCCTCGTAAGAAAGTCAAAAAGACAGTGGTTGATGGCATCGCCCACATCCATGCTTCTTTCAACAACACCATCGTGACCATCACCGACCGTc                                                                                                                                     <  4:302093/149‑1 (MQ=255)
               gCCGATCCGCAAGTAATCGCCCAGGAATATAGACATGGCAAAACCTGCTGCTCGTCCTCGTAAGAAAGTCAAAAAGACAGTGGTTGATGGCATCGCCCACATCCATGCTTCTTTCAACAACACCATCGTGACCATCACCGACCGt                                                                                                                                      >  1:28058/1‑145 (MQ=255)
               gCCGATCCGCAAGTAATCGCCCAGGAATATAGACATGGCAAAACCTGCTGCTCGTCCTCGTAAGAAAGTCAAAAAGACAGTGGTTGATGGCATCGCCCACATCCATGCTTCTTTCAACAACACCATCGTGACCATCACCGACCGt                                                                                                                                      <  2:28058/145‑1 (MQ=255)
                ccGATCCGCAAGTAATCGCCCAGGAATATAGACATGGCAAAACCTGCTGCTCGTCCTCGTAAGAAAGTCAAAAAGACAGTGGTTGATGGCATCGCCCACATCCATGCTTCTTTCAACAACACCATCGTGACCATCaccgac                                                                                                                                         <  8:259943/141‑1 (MQ=255)
                ccGATCCGCAAGTAATCGCCCAGGAATATAGACATGGCAAAACCTGCTGCTCGTCCTCGTAAGAAAGTCAAAAAGACAGTGGTTGATGGCATCGCCCACATCCATGCTTCTTTCAACAACACCATCGTGACCATCaccgac                                                                                                                                         >  7:259943/1‑141 (MQ=255)
                    tCCGCAAGTAATCGCCCAGGAATATAGACATGGCAAAACCTGCTGCTCGTCCTCGTAAGAAAGTCAAAAAGACAGTGGTTGATGGCATCGCCCACATCCATGCTTCTTTCAACAACACCATCGTGACCATCaccgac                                                                                                                                         <  3:229067/137‑1 (MQ=255)
                    tCCGCAAGTAATCGCCCAGGAATATAGACATGGCAAAACCTGCTGCTCGTCCTCGTAAGAAAGTCAAAAAGACAGTGGTTGATGGCATCGCCCACATCCATGCTTCTTTCAACAACACCATCGTGACCATCaccgac                                                                                                                                         >  4:229067/1‑137 (MQ=255)
                        cAAGTAATCGCCCAGGAATATAGACATGGCAAAACCTGCTGCTCGTCCTCGTAAGAAAGTCAAAAAGACAGTGGTTGATGGCATCGCCCACATCCATGCTTCTTTCCACAACACCATCGTGACCATCACCGACCGTCAGGGCAATGCtt                                                                                                                         <  5:247942/149‑1 (MQ=255)
                        cAAGTAATCGCCCAGGAATATAGACATGGCAAAACCTGCTGCTCGTCCTCGTAAGAAAGTCAAAAAGACAGTGGTTGATGGCATCGCCCACATCCATGCTTCTTTCAACAACACCATCGTGACCATCACCGACCGTCAGGGCAATGCtt                                                                                                                         >  8:327144/1‑149 (MQ=255)
                         aaGTAATCGCCCAGGAATATAGACATGGCAAAACCTGCTGCTCGTCCTCGTAAGAAAGTCAAAAAGACAGTGGTTGATGGCATCGCCCACATCCATGCTTCTTTCAACAACACCATCGTGACCATCACCGACCGTCAGGGCAATGCttt                                                                                                                        <  5:71899/149‑1 (MQ=255)
                         aaGTAATCGCCCAGGAATATAGACATGGCAAAACCTGCTGCTCGTCCTCGTAAGAAAGTCAAAAAGACAGTGGTTGATGGCATCGCCCACATCCATGCTTCTTTCAACAACACCATCGTGACCATCACCGACCGTCAGGGCAATGCttt                                                                                                                        >  6:71899/1‑149 (MQ=255)
                           gTAATCGCCCAGGAATATAGACATGGCAAAACCTGCTGCTCGTCCTCGTAAGAAAGTCAAAAAGACAGTGGTTGATGGCATCGCCCACATCCATGCTTCTTTCAACAACACCATCGTGACCATCACCGACCGTCAGGGCAATGCTTTGt                                                                                                                      >  2:276627/1‑149 (MQ=255)
                               tCGCCCAGGAATATAGACATGGCAAAACCTGCTGCTCGTCCTCGTAAGAAAGTCAAAAAGACAGTGGTTGATGGCATCGCCCACATCCATGCTTCTTTCAAAAACACCATCGTGACCATCACCGACCGTCAGGGCAATGCTTTGTCCTg                                                                                                                  >  3:204774/1‑149 (MQ=255)
                                   ccAGGAATATAGACATGGCAAAACCTGCTGCTCGTCCTCGTAAGAAAGTCAAAAAGACAGTGGTTGATGGCATCGCCCACATCCATGCTTCTTTCAACAACACCATCGTGACCATCACCGACCGTCAGGGCAATGCTTTGTCCTggg                                                                                                                <  8:211856/147‑1 (MQ=255)
                                     aGGAATATAGACATGGCAAAACCTGCTGCTCGTCCTCGTAAGAAAGTCAAAAAGACAGTGGTTGATGGCATCGCCCACATCCATGCTTCTTTCAACAACACCATCGTGACCATCACCGACCGTCAGGGCAATGCTTTGTCCTGGGCGAc                                                                                                            <  7:210954/149‑1 (MQ=255)
                                          tataGACATGGCAAAACCTGCTGCTCGTCCTCGTAAGAAAGTCAAAAAGACAGTGGTTGATGGCATCGCCCACATCCATGCTTCTTTCAACAACACCATCGTGACCATCACCGACCGTCAGGGCAATGCTTTGTCCTGGGCGACCTCCg                                                                                                       >  8:91895/1‑149 (MQ=255)
                                            taGACATGGCAAAACCTGCTGCTCGTCCTCGTAAGAAAGTCAAAAAGACAGTGGTTGATGGCATCGCCCACATCCATGCTTCTTTCAACAACACCATCGTGACCATCACCGACCGTCAGGGCAATGCTTTGTCCTGGGCGACCTCCggt                                                                                                     >  8:5311/1‑149 (MQ=255)
                                             aGACATGGCAAAACCTGCTGCTCGTCCTCGTAAGAAAGTCAAAAAGACAGTGGTTGATGGCATCGCCCACATCCATGCTTCTTTCAACAACACCATCGTGACCATCACCGACCGTCAGGGCAATGCTTTGTc                                                                                                                     <  3:18142/132‑1 (MQ=255)
                                             aGACATGGCAAAACCTGCTGCTCGTCCTCGTAAGAAAGTCAAAAAGACAGTGGTTGATGGCATCGCCCACATCCATGCTTCTTTCAACAACACCATCGTGACCATCACCGACCGTCAGGGCAATGCTTTGTc                                                                                                                     >  4:18142/1‑132 (MQ=255)
                                              gACATGGCAAAACCTGCTGCTCGTCCTCGTAAGAAAGTCAAAAAGACAGTGGTTGATGGCATCGCCCACATCCATGCTTCTTTCAACAACACCATCGTGACCATCACCGACCGTCAGGGCAATGCTTTGTCCTGGGCGACCTCCggtg                                                                                                    <  2:96343/148‑1 (MQ=255)
                                                 aTGGCAAAACCTGCTGCTCGTCCTCGTAAGAAAGTCAAAAAGACAGTGGTTGATGGCATCGCCCACATCCATGCTTCTTTCAACAACACCATCGTGACCATCACCGACCGTCAGGGCAATGCTTTGTCCTGGGCGACCTCCGGTGGTTc                                                                                                <  7:91895/149‑1 (MQ=255)
                                                    gctaaaCCTGCTGCTCGGCCTCGTAAGAACGTCAACAAGACAGTGGCTGAGGGCAGCGCCCACATCCATGCTTCTTTCAACAACACCATCGTGACCATCACCGACCGTCAGGGCAATGCTTTGTCCTGGGCGACCTCCGGTGGTTCgg                                                                                              <  2:71053/145‑1 (MQ=255)
                                                    gCAAAACCTGCTGCTCGTCCTCGTAAGAAAGTCAAAAAGACAGTGGTTGATGGCATCGCCCACATCCATGCTTCTTTCAACAACACCATCGTGACCATCACCGACCGTCAGGGCAATGCTTTGTCCTGGGCGACCTCCGGTGGTTCgg                                                                                              <  7:111381/148‑1 (MQ=255)
                                                    gCAAAACCTGCTGCTCGTCCTCGTAAGAAAGTCAAAAAGACAGTGGTTGATGGCATCGCCCACATCCATGCTTCTTTCAACAACACCATCGTGACCATCACCGACCGTCAGGGCAATGCTTTGTCCTGGGCGACCTCCGGTGGTTCgg                                                                                              <  7:327144/148‑1 (MQ=255)
                                                        aaCCTGCTGCTCGTCCTCGTAAGAAAGTCAAAAAGACAGTGGTTGATGGCATCGCCCACATCCATGCTTCTTTCAACAACACCATCGTGACCATCACCGACCGTCAGGGCAATGCTTTGTCCTGGGCGACCTCCGGTGGTTCGGGTTTc                                                                                         <  4:162799/149‑1 (MQ=255)
                                                              ctgctCGTCCTCGTAAGAAAGTCAAAAATACAGTGGTTGATGGCATCGCCCACATCCATGCTTCTTTCAACAACACCATCGTGACCATCACCGACCGTCAGGGCAATGCTTTGTCCTGGGCGACCTCCGGTGGTTCGGGTTTCCGTg                                                                                     <  3:291391/147‑1 (MQ=255)
                                                              ctgctCGTCCTCGTAAGAAAGTCAAAAAGACAGTGGTTGATGGCATCGCCCACATCCATGCTTCTTTCAACAACACCATCGTGACCATCACCGACCGTCAGGGCAATGCTTTGTCCTGGGCGACCTCCg                                                                                                       >  7:221494/1‑129 (MQ=255)
                                                              ctgctCGTCCTCGTAAGAAAGTCAAAAAGACAGTGGTTGATGGCATCGCCCACATCCATGCTTCTTTCAACAACACCATCGTGACCATCACCGACCGTCAGGGCAATGCTTTGTCCTGGGCGACCTCCg                                                                                                       <  8:221494/129‑1 (MQ=255)
                                                                 ctCGTCCTCGTAAGAAAGTCAAAAAGACAGTGGTTGATGGCATCGCCCACATCCATGCTTCTTTCAACAACACCATCGTGACCATCACCGACCGTCAGGGCAATGCTTTGTCCTGGGCGACCTCCgg                                                                                                      >  6:46786/1‑127 (MQ=255)
                                                                 ctCGTCCTCGTAAGAAAGTCAAAAAGACAGTGGTTGATGGCATCGCCCACATCCATGCTTCTTTCAACAACACCATCGTGACCATCACCGACCGTCAGGGCAATGCTTTGTCCTGGGCGACCTCCgg                                                                                                      <  5:46786/127‑1 (MQ=255)
                                                                    gTCCTCGTAAGAAAGTCAAAAAGACAGTGGTTGATGGCATCGCCCACATCCATGCTTCTTTCAACAACACCATCGTGACCATCACCGACCGTCAGGGCAATGCTTTGTCCTGGGCGACCTCCGGTGGTTCGGGTTTCCGTGGTTCgcgc                                                                             <  2:258429/149‑1 (MQ=255)
                                                                       cTCGTAAGAAAGTCAAAAAGACAGTGGTTGATGGCATCGCCCACATCCATGCTTCTTTCAACAACACCATCGTGACCATCACCGACCGTCAGGGCAATGCTTTGTCCTGGGCGACCTCCGGTGGTTCGGGTTTCCGTGGTTCGCGCaaa                                                                          >  7:323165/1‑149 (MQ=255)
                                                                          gTAAGAAAGTCAAAAAGACAGTGGTTGATGGCATCGCCCACATCCATGCTTCTTTCAACAACACCATCGTGACCATCACCGACCGTCAGGGCAATGCTTTGTCCTGGGCGACCTCCGGTGGTTCGGGTTTCCGTGGTTCGCGCAAATcc                                                                       <  1:276627/149‑1 (MQ=255)
                                                                             aGAAAGTCAAAAAGACAGTGGTTGATGGCATCGCCCACATCCATGCTTCTTTCAACAACACCATCGTGACCATCACCGACCGTCAGGGCAATGCTTTGTCCTGGGCGACCTCCGGTGGTTCGGGTTTCCGTGGTTCGCGCAAATCCAcc                                                                    <  7:5311/149‑1 (MQ=255)
                                                                              gAAAGTCAAAAAGACAGTGGTTGATGGCATCGCCCACATCCATGCTTCTTTCAACAACACCATCGCGACCATCACCGACCGTCAGGGCAATGCTTTGTCCTGGGCGACCTCCGGTGGTTCGGGTTTCCGTGGTTCGCGCAAATCCAccc                                                                   <  4:171973/149‑1 (MQ=255)
                                                                                     aaaaaGACAGTGGTTGATGGCATCGCCCACATCCATGCTTCTTTCAACAACACCATCGTGACCATCACCGACCGTCAGGGCAATGCTTTGTCCTGGGCGACCTCCGGTGGTTCgg                                                                                              <  4:236540/115‑1 (MQ=255)
                                                                                     aaaaaGACAGTGGTTGATGGCATCGCCCACATCCATGCTTCTTTCAACAACACCATCGTGACCATCACCGACCGTCAGGGCAATGCTTTGTCCTGGGCGACCTCCGGTGGTTCGGGTTTCCGTGGTTCGCGCAAATCCACCCCGTTCg                                                             >  2:249057/1‑148 (MQ=255)
                                                                                      aaaaGACAGTGGTTGATGGCATCGCCCACATCCATGCTTCTTTCAACAACACCATCGCGACCATCACCGACCGTCAGGGCAATGCTTTGTCCTGGGCGACCTCCGGTGGTTCGGGTTTCCGTGGTTCGCGCAAATCCACCCCGTTCg                                                             >  7:327548/1‑147 (MQ=255)
                                                                                       aaaGACAGTGGTTGATGGCATCGCCCACATCCATGCTTCTTTCAACAACACCATCGTGACCATCACCGACCGTCAGGGCAATGCTTTGTCCTGGGCGACCTCCGGTGGTTCGGGTTTCCGTGGTTCGCGCAAATCCAcc                                                                    >  1:132790/1‑139 (MQ=255)
                                                                                       aaaGACAGTGGTTGATGGCATCGCCCACATCCATGCTTCTTTCAACAACACCATCGTGACCATCACCGACCGTCAGGGCAATGCTTTGTCCTGGGCGACCTCCGGTGGTTCGGGTTTCCGTGGTTCGCGCAAATCCAcc                                                                    <  2:132790/139‑1 (MQ=255)
                                                                                          gACAGTGGTTGATGGCATCGCCCACATCCATGCTTCTTTCAACAACACCATCGTGACCATCACCGACCGTCAGGGCAATGCTTTGTCCTGGGCGACCTCCGGTGGTTCGGGTTTCCGTGGTTCGCGCAAATCCACCCCGTTCGCAGCt                                                        >  6:311740/1‑148 (MQ=255)
                                                                                                 gTTGATGGCATCGCCCACATCCATGCTTCTTTCAACAACACCATCGTGACCATCACCGACCGTCAGGGCAATGCTTTGTCCTGGGCGACCTCCGGTGGTTCGGGTTTCCGTGGtt                                                                                  <  2:20709/115‑1 (MQ=255)
                                                                                                 gTTGATGGCATCGCCCACATCCATGCTTCTTTCAACAACACCATCGTGACCATCACCGACCGTCAGGGCAATGCTTTGTCCTGGGCGACCTCCGGTGGTTCGGGTTTCCGTGGtt                                                                                  >  1:20709/1‑115 (MQ=255)
                                                                                                 gTTGATGGCATCGCCCACATCCATGCTTCTTTCAACAACACCATCGTGACCATCACCGACCGTCAGGGCAATGCTTTGTCCTGGGCGACCTCCGGTGGTTCGGGTTTCCGTGGTTCGCGCAAATCCACCCCGTTCGCAGCTCAGATCg                                                 <  8:323165/148‑1 (MQ=255)
                                                                                                      tGGCATCGCCCACATCCATGCTTCTTTCAACAACACCATCGTGACCATCACCGACCGTCAGGGCAATGCTTTGTCCTGGGCGACCTCCGGTGGTTCGGGTTTCCGTGGTTCGCGCAAATCCACCCCGTTCGCAGCTCAGATCgctgct                                            >  4:291259/1‑148 (MQ=255)
                                                                                                      tGGCATCGCCCACATCCATGCTTCTTTCAACAACACCATCGTGACCATCACCGACCGTCAGGGCAATGCTTTGTCCTGGGCGACCTCCGGTGGTTCGGGTTTCCGTGGTTCGCGCAAATCCACCCCGTTCGCAGCTCAGATCgccgct                                            >  6:283132/1‑148 (MQ=255)
                                                                                                             gCCCACATCCATGCTTCTTTCAACAACACCATCGCGACCATCACCGACCGTCAGGGCAATGCTTTGTCCTGGGCGACCTCCGGTGGTTCGGGTTTCCGTGGTTCGCGCAAATCCACCCCGTTCGCAGCTCAGATCGCTGCTGAGCGTg                                     <  8:327548/148‑1 (MQ=255)
                                                                                                               ccACATCCATGCTTCTTTCAACAACACCATCGTGACCATCACCGACCGTCAGGGCAATGCTTTGTCCTGGGCGACCTCCGGTGGTTCGGGTTTCCGTGGTTCGCGCAAATCCACCCCGTTCGCAGCTc                                                       >  4:303201/1‑128 (MQ=255)
                                                                                                                cacaTCCATGCTTCTTTCAACAACACCATCGTGACCATCACCGACCGTCAGGGCAATGCTTTGTCCTGGGCGACCTCCGGTGGTTCGGGTTTCCGTGGTTCGCGCAAATCCACCCCGTTCGCAGc                                                         <  1:113742/125‑1 (MQ=255)
                                                                                                                cacaTCCATGCTTCTTTCAACAACACCATCGTGACCATCACCGACCGTCAGGGCAATGCTTTGTCCTGGGCGACCTCCGGTGGTTCGGGTTTCCGTGGTTCGCGCAAATCCACCCCGTTCGCAGc                                                         >  2:113742/1‑125 (MQ=255)
                                                                                                                     ccATGCTTCTTTCAACAACACCATCGTGACCATCACCGACCGTCAGGGCAATGCTTTGTCCTGGGCGACCTCCGGTGGTTCGGGTTTCCGTTGTTCGCGCAAATCCACCCCGTTCGCAGCTCAGATCGCTGCTGAGCGTGCTGGTCAAg                            >  3:71674/1‑149 (MQ=255)
                                                                                                                       aTGCTTCTTTCAACAACACCATCGTGACCATCACCGACCGTCAGGGCAATGCTTTGTCCTGGGCGACCTCCGGTGGTTCGGGTTTCCGTGGTTCGCGCAAATCCACCCCGTTCGCAGCTCAGATCGCTGCTGAGCGTGCTGGTCAAGCt                          >  6:157229/1‑149 (MQ=255)
                                                                                                                         gCTTCTTTCAACAACACCATCGTGACCATCACCGACCGTCAGGGCAATGCTTTGTCCTGGGCGACCTCCGGTGGTTCGGGTTTCCGTGGTTCGCGCAAATCCACCCCGTTCGCAGCTCAGATCGCTGCTGAGCGTGCTGGTCAAGCTgc                        >  7:110914/1‑149 (MQ=255)
                                                                                                                          cttcttTCAACAACACCATCGTGACCATCACCGACCGTCAGGGCAATGCTTTGTCCTGGGCGACCTCCGGTGGTTCGGGTTTCCGTGGTTCGCGCAAATCCACCCCGTTCGCAGCTCAGATCGCTGCTGAGCGTGCTGGTCAAGCTgcg                       >  3:58225/1‑149 (MQ=255)
                                                                                                                          cttcttTCAACAACACCATCGTGACCATCACCGACCGTCAGGGCAATGCTTTGTCCTGGGCGACCTCCGGTGGTTCGGGTTTCCGTGGTTCGCGCAAATCCACCCCGTTCGCAGCTCAGATCGCTGCTGAGCGTGCTGGTCAAGCTgc                        <  3:291259/148‑1 (MQ=255)
                                                                                                                             cttTCAACAACACCATCGTGACCATCACCGACCGTCAGGGCAATGCTTTGTCCTGGGCGACCTCCGGTGGTTCGGGTTTCCGTGGTTCGCGCAAATCCACCCGTTTCGCAGCTCAGATCGCTGCTGAGCGTGCTGGTCAAGCTGCGCt                     <  1:249057/148‑1 (MQ=255)
                                                                                                                             cttTCAACAACACCATCGTGACCATCACCGACCGTCAGGGCAATGCTTTGTCCTGGGCGACCTCCGGTGGTTCGGGTTTCCGTGGTTCGCGCAAATCCACCCCGTTCGCAGCTCAGATCGCCGCTGAGCGTGCTGGTCAAGCTgcgc                      <  5:283132/147‑1 (MQ=255)
                                                                                                                                   acaacaCCATCGTGACCATCACCGACCGTCAGGGCAATGCTTTGTCCTGGGCGACCTCCGGTGGTTCGGGTTTCCGTGGTTCGCGCAAATCCACCCCGTTCGCAGCTCAGATCGCTGCTGAGCGTGCTGGTCAAGCTGCGCTGGAATAc              >  7:233141/1‑149 (MQ=255)
                                                                                                                                    caacaCCATCGTGACCATCACCGACCGTCAGGGCAATGCTTTGTCCTGGGCGACCTCCGGTGGTTCGGGTTTCCGTGGTTCGCGCAAATCCACCCCGTTCGCAGCTCAGATCGCTGCTGAGCGTGCTGGTCAAGCTGCGCTGGAATACg             <  5:157229/149‑1 (MQ=255)
                                                                                                                                     aacaCCATCGTGACCATCACCGACCGTCAGGGCAATGCTTTGTCCTGGGCGACCTCCGGTGGTTCGGGTTTCCGTGGTTCGCGCAAATCCACCCCGTTCGCAGCTCAGATCGCTGCTGAGCGTGCTGGTCAAGCTGCACTGGAATACgg            >  3:195354/1‑149 (MQ=255)
                                                                                                                                     aacaCCATCGTGACCATCACCGACCGTCAGGGCAATGCTTTGTCCTGGGCGAACTCCCGTGGTTCGGGTTTCCGTGGTTCGCGCAAATCCAACCCGTTCGCAGCTCAGATCGCTCATTACCGTGCTGGTCAAGCTGCCCTGGAATACgg            >  4:306725/1‑149 (MQ=255)
                                                                                                                                       cacCATCGTGACCATCACCGACCGTCAGGGCAATGCTTTGTCCTGGGCGACCTCCGGTGGTTCGGGTTTCCGTGGTTCGCGCAAATCCACCCCGTTCGCAGCTCAGATCGCTGCTGAGCGTGCTGGTCAAGCTGCGCTGGAATACGGTc          >  2:190089/1‑149 (MQ=255)
                                                                                                                                        acCATCGTGACCATCACCGACCGTCAGGGCAATGCTTTGTCCTGGGCGACCTCCGGTGGTTCGGGTTTCCGTGGTTCGCGCAAATCCACCCCGTTCGCAGCTCAGATCGCTGCTGa                                          >  6:19822/1‑116 (MQ=255)
                                                                                                                                        acCATCGTGACCATCACCGACCGTCAGGGCAATGCTTTGTCCTGGGCGACCTCCGGTGGTTCGGGTTTCCGTGGTTCGCGCAAATCCACCCCGTTCGCAGCTCAGATCGCTGCTGa                                          <  5:19822/116‑1 (MQ=255)
                                                                                                                                               tGACCATCACCGACCGTCAGGGCAATGCTTTGTCCTGGGCGACCTCCGGTGGTTCGGGTTTCCGTGGTTCGCGCAAATCCACCCCGTTCGCAGCTCAGATCGCTGCTGAGCGTGCTGGTCAAGCTGCGCTGGAATACGGTCTGAAGAAc  <  3:306725/149‑1 (MQ=255)
                                                                                                                                               |                                                                                                                                                    
TAAGGGCCCGCGTAAGCCGATCCGCAAGTAATCGCCCAGGAATATAGACATGGCAAAACCTGCTGCTCGTCCTCGTAAGAAAGTCAAAAAGACAGTGGTTGATGGCATCGCCCACATCCATGCTTCTTTCAACAACACCATCGTGACCATCACCGACCGTCAGGGCAATGCTTTGTCCTGGGCGACCTCCGGTGGTTCGGGTTTCCGTGGTTCGCGCAAATCCACCCCGTTCGCAGCTCAGATCGCTGCTGAGCGTGCTGGTCAAGCTGCGCTGGAATACGGTCTGAAGAAC  >  NC_002947/561821‑562112

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑