Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 587,076 C→G 6.8% P43R (CCG→CGG)  PP_0497 → Cro/CI family transcriptional regulator

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_002947587,0760CG 6.8% 176.9 / 3.0 58P43R (CCG→CGG) PP_0497Cro/CI family transcriptional regulator
Reads supporting (aligned to +/- strand):  ref base C (22/32);  new base G (2/2);  total (24/34)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.12e-01

TCACCTGGATTCCGCATATGAGCGATCACTTCGCTACAAACCTCAAACTTGCCTGCAGTCACTACCGCTCTATTTCCGAAGTTTGCCGCCAGCTGTCGATCAACCGCGCGCAGTTCAACAAGTACCTGAGCGGGCAAAGCCGTCCGACGGCTTTCAACCTGAAGCGTATCGGCGACTTCTTCGGTGTCGAGGATTACGAACTGAACCTGCCCGCCGAGCAGTTCGCCCGTCTGATCGGTGCGCGGGTATCGCCCCAGGCCCAGCAACCCAGTGACCCGATCAGCGAGTTGTT  >  NC_002947/586932‑587223
                                                                                                                                                |                                                                                                                                                   
tCACCTGGATTCCGCATATGAGCGATCACTTCGCTACAAACCTCAAACTTGCCTGCAGTCACTACCGCTCTATTTCCGAAGTTTGCCGCCAGCTGTCGATCAACCGCGCGCAGTTCAACAAGTACCTGAGCGGGCAAAGCCGTCCGACg                                                                                                                                                 <  1:22698/149‑1 (MQ=255)
  aCCTGGATTCCGCATATGAGCGATCACTTCGCTACAAACCTCAAACTTGCCTGCAGTCACTACCGCTCTATTTCCGAAGTTTGCCGCCAGCTGTCGATCAACCGCGCGCAGTTCAACAAGTACCTGAGCGGGCAAAGCCGTCCGACGGc                                                                                                                                               >  4:344183/1‑149 (MQ=255)
    cTGGATTCCGCATATGAGCGATCACTTCGCTACAAACCTCAAACTTGCCTGCAGTCACTACCGCTCTATTTCCGAAGTTTGCCGCCAGCTGTCGATCAACCGCGCGCAGTTCAACAAGTACCTGAGCGGGCAAAGCCGTCGGACGGCtt                                                                                                                                             >  8:70430/1‑149 (MQ=255)
    cTGGATTCCGCATATGAGCGATCACTTCGCTACAAACCTCAAACTTGCCTGCAGTCACTACCGCTCTATTTCCGAAGTTTGCCGCCAGCTGTCGATCAACCGCGCGCAGTTCAACAAGTACCTGAGCGGGCAAAGCCGTCCGACGGCtt                                                                                                                                             >  3:181486/1‑149 (MQ=255)
    cTGGATTCCGCATATGAGAGATCACTTCGCTACAAACCTCAAACTTGCCTGCAGTCACTACCGCTCTATTTCCGAAGTTTGCCGCCAGCTGTCGATCAACCGCGCGCAGTTCAACAAGTACCTGAGCGGGCAAAGCCGTCCGACGGCtt                                                                                                                                             <  4:253160/149‑1 (MQ=255)
       gATTCCGCATATGAGCGATCACTTCGCTACAAACCTCAAACTTGCCTGCAGTCACTACCGCTCTATTTCCGAAGTTTGCCGCCAGCTGTCGATCAACCGCGCGCAGTTCAACAAGTACCTGAGCGGGCAAAGCCGTCGGACGGCttt                                                                                                                                            <  7:70430/147‑1 (MQ=255)
       gATTCCGCATATGAGCGATCACTTCGCTACAAACCTCAAACTTGCCTGCAGTCACTACCGCTCTATTTCCGAAGTTTGCCGCCAGCTGTCGATCAACCGCGCGCAGTTCAACAAGTACCTGAGCGGGCAAAGCCGTCCGACGGCttt                                                                                                                                            <  6:56046/147‑1 (MQ=255)
       gATTCCGCATATGAGCGATCACTTCGCTACAAACCTCAAACTTGCCTGCAGTCACTACCGCTCTATTTCCGAAGTTTGCCGCCAGCTGTCGATCAACCGCGCGCAGTTCAACAAGTACCTGAGCGGGCAAAGCCGTCCGACGGCTTTc                                                                                                                                           <  3:117489/148‑1 (MQ=255)
        aTTCCGCATATGAGCGATCACTTCGCTACAAACCTCAAACTTGCCTGCAGTCACTACCGCTCTATTTCCGAAGTTTGCCGCCAGCTGTCGATCAACCGCGCGCAGTTCAACAAGTACCTGAGCGGGCAAAGCCGTCCGACGGCTTTCaa                                                                                                                                         <  1:312661/149‑1 (MQ=255)
         ttCCGCATATGAGCGATCACTTCGCTACAAACCTCAAACTTGCCTGCAGTCACTACCGCTCTATTTCCGAAGTTTGCCGCCAGCTGTCGATCAACCGCGCGCAGTTCAACAAGTACCTGAGCGGGCAAAGCCGTCCGACGGCTTTCAAc                                                                                                                                        <  3:322291/149‑1 (MQ=255)
              cATATGAGCGATCACTTCGCTACAAACCTCAAACTTGCCTGCAGTCACTACCGCTCTATTTCCGAAGTTTGCCGCCAGCTGTCGATCAACCGCGCGCAGTTCAACAAGTACCTGAGCGGGCAAAGCCGTCCGACGGCTTTCAACCTGaa                                                                                                                                   <  3:344183/149‑1 (MQ=255)
                             atCGCTACAAACCTCAAACTTGCCTGCAGTCACTACCGCTCTATTTCCGAAGTTTGCCGCCAGCTGTCGATCAACCGCGCGCAGTTCAACAAGTACCTGAGCGGGCAAAGCCGTCCGACGGCTTTCAACCTGAAGCGTATCGGCGAct                                                                                                                     >  4:227883/2‑148 (MQ=255)
                                 cTACAAACCTCAAACTTGCCTGCAGTCACTACCGCTCTATTTCCGAAGTTTGCCTCCAGCTGTCGATCAACCGCGCGCAGTTCAACAAGTACCTGAGCGGGCAAAGCCGTCCGACGGCTTTCAACCTGAAGCGTATCGGCGACttcttc                                                                                                                <  4:181486/149‑1 (MQ=255)
                                        ccTCAAACTTGCCTGCAGTCACTACCGCTCTATTTCCGAAGTTTGCCGCCAGCTGTCGATCAACCGCGCGCAGTTCAACAAGTACCTGAGCGGGCAAAGCCGTCCGACGGCTTTCAAc                                                                                                                                        <  2:342915/118‑1 (MQ=255)
                                        ccTCAAACTTGCCTGCAGTCACTACCGCTCTATTTCCGAAGTTTGCCGCCAGCTGTCGATCAACCGCGCGCAGTTCAACAAGTACCTGAGCGGGCAAAGCCGTCCGACGGCTTTCAAc                                                                                                                                        >  1:342915/1‑118 (MQ=255)
                                         cTCAAACTTGCCTGCAGTCACTACCGCTCTATTTCCGAAGTATGCCGCCAGCTGTCGATCAACCGCGCGCAGTTCAACAAGTACCTGAGCGGGCAAAGCCGTCCGACGGCTTTCAACCTGAAGCGTATCGGCGACTTCTTCGGTGTCGa                                                                                                        <  5:307100/149‑1 (MQ=255)
                                          tCAAACTTGCCTGCAGTCACTACCGCTCTATTTCCGAAGTTTGCCGCCAGCTGTCGATCAACCGCGCGCAGTTCAACAAGTACCTGAGCGGGCAAAGCCGTCCGACGGCTTTCAACCTGAAGCGTATCGGCGACTTCTTCGGTGTCGAg                                                                                                       >  2:284930/1‑149 (MQ=255)
                                            aaaCTTGCCTGCAGTCACTACCGCTCTATTTCCGAAGTTTGCCGCCAGCTGTCGATCAACCGCGCGCAGTTCAACAAGTACCTGAGCGGGCAAAGCCGTCCGACGGCTTTCAACCTGAAGCGTATCGGCGACTTCTTCGGTGTCGAg                                                                                                       <  3:227883/147‑1 (MQ=255)
                                                     tGCAGTCACTACCGCTCTATTTCCGAAGTTTGCCGCCAGCTGTCGATCAACCGCGCGCAGTTCAACAAGTACCTGAGCGGGCAAAGCCGTCCGACGGCTTTCAAcc                                                                                                                                       <  5:182462/106‑1 (MQ=255)
                                                     tGCAGTCACTACCGCTCTATTTCCGAAGTTTGCCGCCAGCTGTCGATCAACCGCGCGCAGTTCAACAAGTACCTGAGCGGGCAAAGCCGTCCGACGGCTTTCAAcc                                                                                                                                       >  6:182462/1‑106 (MQ=255)
                                                       cactCACTACCGCTCTATTTCCGAAGTTTGCCGCCAGCTGTCGATCAACCGCGCGCAGTTCAACAAGTACCTGAGCGGGCAAAGCCGTCCGACGGCTTTCAACCTGAAGCGTATCGGCGACTTCTTCGGTGTCGAGGATTACGAACTg                                                                                           <  8:324420/145‑1 (MQ=255)
                                                       cAGTCACTACCGCTCTATTTCCGAAGTTTGCCGCCAGCTGTCGATCAACCGCGCGCAGTTCAACAAGTACCTGAGCGGGCAAAGCCGTCCGACGGCTTTCAACCTGAAGCGTATCGGCGACTTCTTCGGTGTCGAGGATTACGAACTg                                                                                           >  3:314808/1‑148 (MQ=255)
                                                        aGTCACTACCGCTCTATTTCCGAAGTTTGCCGCCAGCTGTCGATCAACCGCGCGCAGTTCAACAAGTACCTGAGCGGGCAAAGCCGTCCGACGGCTTTCAACCTGAAGCGTATCGGCGACTTCTTCGGTGTCGAGGATTACGAACTGaa                                                                                         >  1:156361/1‑149 (MQ=255)
                                                          tCACTACCGCTCTATTTCCGAAGTTTGCCGCCAGCTGTCGATCAACCGCGCGCAGTTCAACAAGTACCTGAGCGGGCAAAGCCGTCCGACGGCTTTCAACCTGAAGCGTATc                                                                                                                            <  3:192258/112‑1 (MQ=255)
                                                          tCACTACCGCTCTATTTCCGAAGTTTGCCGCCAGCTGTCGATCAACCGCGCGCAGTTCAACAAGTACCTGAGCGGGCAAAGCCGTCCGACGGCTTTCAACCTGAAGCGTATc                                                                                                                            >  4:192258/1‑112 (MQ=255)
                                                            aCTACCGCTCTATTTCCGAAGTTTGCCGCCAGCTGTCGATCAACCGCGCGCAGTTCAACAAGTACCTGAGCGGGCAAAGCCGTCCGACGGCTTTCAACCTGaa                                                                                                                                   <  3:99728/103‑1 (MQ=255)
                                                            aCTACCGCTCTATTTCCGAAGTTTGCCGCCAGCTGTCGATCAACCGCGCGCAGTTCAACAAGTACCTGAGCGGGCAAAGCCGTCCGACGGCTTTCAACCTGaa                                                                                                                                   >  4:99728/1‑103 (MQ=255)
                                                                ccGCTCTATTTCCGAAGTTTGCCGCCAGCTGTCGATCAACCGCGCGCAGTTCAACAAGTACCTGAGCGGGCAAAGCCGTCCGACGGCTTTCAACCTGAAGCGTATCGGCGACTTCTTCGGTGTCGAGGATTACGAACTGAACCTGcc                                                                                   <  4:314808/147‑1 (MQ=255)
                                                                      tataTCCGAAGTTTGCCGCCAGCTGTCGATCAACCGCGCGCAGTTCAACAAGTACCTGAGCGGGCAAAGCCGTCCGACGGCTTTCAACCTGAAGCGTATCGGCGACTTCTTCGGTGTCGAGGATTACGAACTGAACCTGCCCGCCGa                                                                             <  1:262661/147‑1 (MQ=255)
                                                                      tATTTCCGAAGTTTGCCGCCAGCTGTCGATCAACCGCGCGCAGTTCAACAAGTACCTGAGCGGGCAAAGCCGTCCGACGGCTTTCAACCTGAAGCGTATCGGCGACTTCTTCg                                                                                                               <  6:11677/113‑1 (MQ=255)
                                                                      tATTTCCGAAGTTTGCCGCCAGCTGTCGATCAACCGCGCGCAGTTCAACAAGTACCTGAGCGGGCAAAGCCGTCCGACGGCTTTCAACCTGAAGCGTATCGGCGACTTCTTCg                                                                                                               >  5:11677/1‑113 (MQ=255)
                                                                      tATTTCCGAAGTTTGCCGCCAGCTGTCGATCAACCGCGCGCAGTTCAACAAGTACCTGAGCGGGCAAAGCCGTCCGACGGCTTTCAACCTGAAGCGTATCGGCGACTTCTTCGGTGTCGAGGATTACGAACTGAACCTGCCCGCCGa                                                                             <  4:290185/147‑1 (MQ=255)
                                                                           ccGAAGTTTGCCGCCAGCTGTCGATCAACCGCGCGCAGTTCAACAAGTACCTGAGCGGGCAAAGCCGTCCGACGGCTTTCAACCTGAAGCGTATCGGCGACTTCTTCGGTGTCGAGGATTACGAACTGAACCTGCCCGCCGAGCacaa                                                                       <  2:156361/148‑4 (MQ=255)
                                                                           ccGAAGTTTGCCGCCAGCTCTCGATCAACCGCGCGCAGTTCAACAACTACCTGAGCGGGCAAAGCCGTCCGACGGCTTTAAACCTGAAGCGTATCGGCGACTTCTTCGGTGTCGAGGATTACGAACTGAACCTGCCCGCCGAGCAGTTc                                                                      >  2:109871/1‑149 (MQ=255)
                                                                            cGAAGTTTGCCGCCAGCTGTCGATCAACCGCGCGCAGTTCAACAAGTACCTGAGCGGGCAAAGCCGTCCGACGGCTTTCAACCTGAAGCGTATCGGCGACTTCTTCGGTGTCGAGGATTACGAACTGAACCTGCCCGCCGAGCAGTTCg                                                                     >  4:260127/1‑149 (MQ=255)
                                                                                   tGCCGCCAGCTGTCGATCAACCGCGCGCAGTTCAACAAGTACCTGAGTGGGCAAAGCCGTCCGACTGCTTTCAACCTGAAGCGTATCGGCGACTTCTTCGGTGTCGAGGATTACGAACTGAACCTGCCCGCCGAGCAGTTCGCCCGTc                                                               <  2:282908/148‑1 (MQ=255)
                                                                                      cgccAGCTGTCGATCAACCGCGCGCAGTTCAACAAGTACCTGAGCGGGCAAAGCCGTCCGACGGCTTTCaa                                                                                                                                         <  3:347614/71‑1 (MQ=255)
                                                                                      cgccAGCTGTCGATCAACCGCGCGCAGTTCAACAAGTACCTGAGCGGGCAAAGCCGTCCGACGGCTTTCaa                                                                                                                                         >  4:347614/1‑71 (MQ=255)
                                                                                         cAGCTGTCGATCAACCGCGCGCAGTTCAACAAGTACCTGAGCGGGCAAAGCCGTCCGACGGCTTTCAACCTGAAGCGTATCGGCGACTTCTTCGGTGTCGAGGATTACGAACTGAACCTGCCCGCCGAGCAGTTCGc                                                                    <  2:317348/137‑1 (MQ=255)
                                                                                                 gATCAACCGCGCGCAGTTCAACAAGTACCTGAGCGGGCAAAGCCGTCCGACGGCTTTCAACCTGAAGCGTATCGGCGACTTCTTCGGTGTCGAGGATTACGAACTGAACCTGCCCGCCGAGCAGTTCGCCCGTCTGATCGGTGCGCgg                                                 <  1:237209/148‑1 (MQ=255)
                                                                                                       ccGCGCGCAGTTCAACAAGTACCTGAGCGGGCAAAGCCGTCCGACGGCTTTCAACCTGAAGCGTATCGGCGACTTCTTCGGTGTCGAgg                                                                                                      <  4:58264/89‑1 (MQ=255)
                                                                                                       ccGCGCGCAGTTCAACAAGTACCTGAGCGGGCAAAGCCGTCCGACGGCTTTCAACCTGAAGCGTATCGGCGACTTCTTCGGTGTCGAgg                                                                                                      >  3:58264/1‑89 (MQ=255)
                                                                                                       ccGCGCGCAGTTCAACAAGTACCTGAGCGGGCAAAGCCGTCCGACGGCTTTCAACCTGAAGCGTATCGGCGACTTCTTCGGTGTCGAGGATTACGAACTGAACCTGCCCGCCGAGCAGTTCGCCCGTCTGATCGGTGCGCGGGTATCGc                                          <  3:260127/149‑1 (MQ=255)
                                                                                                                      caaGTACCTGAGCGGGCAAAGCCGTCCGACGGCTTTCAACCTGAAGCGTATCGCCGACTTCTTCGGTGTCGAGGATTACGAACTGAACCTGCCCGCCGAGCAGTTCGCCCGTCTGATCGGTGCGCGGGTATCGCCCCAGGCCCAGCAAc                           <  1:284930/149‑1 (MQ=255)
                                                                                                                            ccTGAGCGGGCAAAGCCGTCCGACGGCTTTCAACCTGAAGCGTATCGGCGACTTCTTCGGTGTCGAGGATTACGAACTGAACCTGCCCGCCGAGCAGTTCGCCCGTCTGATCGGTGCGCGGGTATCGCCCCAGGCCCAGCAACCCAGTg                     >  2:309272/1‑149 (MQ=255)
                                                                                                                             cTGAGCGGGCAAAGCCGTCCGACGGCTTTCAACCTGAAGCGTATCGGCGACTTCTTCGGTGTCGAGGATTACGAACTGAACCTGCCCGCCGAGCAGTTCGCCCGTCTGAt                                                           <  6:293158/110‑1 (MQ=255)
                                                                                                                               gAGCGGGCAAAGCCGTCCGACGGCTTTCAACCTGAAGCGTATCGGCGACTTCTTCGGTGTCGAGGATTACGAACTGAAc                                                                                        <  4:203315/79‑1 (MQ=255)
                                                                                                                               gAGCGGGCAAAGCCGTCCGACGGCTTTCAACCTGAAGCGTATCGGCGACTTCTTCGGTGTCGAGGATTACGAACTGAAc                                                                                        >  3:203315/1‑79 (MQ=255)
                                                                                                                                aGCGGGCAAAGCCGTCCGACGGCTTTCAACCTGAAGCGTATCGGCGACTTCTTCGGTGTCGAGGATTACGAACTGAACCTGCCCGCCGAGCAGTTCGCCCGTCTGATCGGTGCGCGGGTATCGCCCCAGGCCCAGCAACCCAGTGAccc                 >  5:351608/1‑149 (MQ=255)
                                                                                                                                  ggttgaaaGCCGTCGGACGGCTTTCAACCTGAAGCGTATCGGCGACTTCTTCGGTGTCGAGGATTACGAACTGAACCTGCCCGCCGAGCAGTTCACCCGTCTGATCGGTGCGCGGGTATCGCCCCAGGCCCAGCAACCCAGTGACCCGa               >  2:198103/6‑149 (MQ=255)
                                                                                                                                  cGGGCAAAGCCCTCCAACGGCTTTCAACCTCAAGCGTATCGGCGACTTCTTCCGTGTCGAGGATTACGAACTGAACCTGCCCGCCCAGCACTTCGCCAGTCTGATCGGTACGCGGTTAACGCCCCAGGCCCAGCAACCCAGTGACCCg                >  8:70513/1‑148 (MQ=255)
                                                                                                                                    ggCAAAGCCGTCCGACGGCTTTCAACCTGAAGCGTATCGGCGACTTCTTCGGTGTCGAGGATTACGAACTGAACCTGCCCGCCGAGCAGTTCGCCCGTCTGATCGGTGCGCGGGTATCGCCCCAGGCCCAGCAACCCAGTGACCCGATc             <  6:351608/149‑1 (MQ=255)
                                                                                                                                      cAAAGCCGTCCGACGGCTTTCAACCTGAAGCGTATCGGCGACTTCTTCGGTGTCGAGGATTACGAACTGAACCTGCCCGCCGAGCAGTTCGCCCGTCTGATCGGTGCGCGGGTATCGCCCCAg                                     >  3:330671/1‑123 (MQ=255)
                                                                                                                                      cAAAGCCGTCCGACGGCTTTCAACCTGAAGCGTATCGGCGACTTCTTCGGTGTCGAGGATTACGAACTGAACCTGCCCGCCGAGCAGTTCGCCCGTCTGATCGGTGCGCGGGTATCGCCCCAg                                     <  4:330671/123‑1 (MQ=255)
                                                                                                                                       aaaGCCGTCCGACGGCTTTCAACCTGAAGCGTATCGGCGACTTCTTCGGTGTCGAGGATTACGAACTGAACCTGCCCGCCGAGCAGTTCGCCCGTCTGATCGGTGCGCGGGTATCGCCCCAGGCCCAGCAACCCAGTGACCCGATCAGc          >  4:10951/1‑149 (MQ=255)
                                                                                                                                          gCCGTCGGACGGCTTTCAACCTGAAGCGTATCGGCGACTTCTTCGGTGTCGAGGATTACGAACTGAACCTGCCCGCCGAGCAGTTCACCCGTCTGATCGGTGCGCGGGTATCGCCCCAGGGCCAGCAACCCAGTGACCCGATCAGCGAg       <  1:198103/149‑1 (MQ=255)
                                                                                                                                              tCCGACGGCTTTCAACCTGAAGCGTATCGGCGACTTCTTCGGTGTCGAGGATTACGAACTGAACCTGCCCGCCGAGCAGTTCGCCCGTCTGATCGGTGCGCGGGTATCGCCCCAGGCCCa                                <  5:337865/120‑1 (MQ=255)
                                                                                                                                              tCCGACGGCTTTCAACCTGAAGCGTATCGGCGACTTCTTCGGTGTCGAGGATTACGAACTGAACCTGCCCGCCGAGCAGTTCGCCCGTCTGATCGGTGCGCGGGTATCGCCCCAGGCCCa                                >  6:337865/1‑120 (MQ=255)
                                                                                                                                                cGACGGCTTTCAACCTGAAGCGTATCGGCGACTTCTTCGGTGTCGAGTATTACGAACTGAACCTGCCCGCTGAGCATTTCGCCCGTCTGATCGGTGCGCGGGTATCGCCCCAGGCCCAGCAACCCAGTGACCCGATCAGCGAgttgtt  <  1:109871/148‑1 (MQ=255)
                                                                                                                                                cGACGGCTTTCAACCTGAAGCGTATCGGCGACTTCTTCGGTGTCGAGGATTACGAACTGAACCTGCCCGCCGAGCAGTTCGCCCGTCTGATCGGTGCGCGGGTATCGCCCCAGGCCCAGCAACCCAGTGACCCGATCAGCGAgttgtt  <  3:10951/148‑1 (MQ=255)
                                                                                                                                                |                                                                                                                                                   
TCACCTGGATTCCGCATATGAGCGATCACTTCGCTACAAACCTCAAACTTGCCTGCAGTCACTACCGCTCTATTTCCGAAGTTTGCCGCCAGCTGTCGATCAACCGCGCGCAGTTCAACAAGTACCTGAGCGGGCAAAGCCGTCCGACGGCTTTCAACCTGAAGCGTATCGGCGACTTCTTCGGTGTCGAGGATTACGAACTGAACCTGCCCGCCGAGCAGTTCGCCCGTCTGATCGGTGCGCGGGTATCGCCCCAGGCCCAGCAACCCAGTGACCCGATCAGCGAGTTGTT  >  NC_002947/586932‑587223

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑