Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 593,433 G→T 11.3% E391* (GAG→TAG) ‡ PP_0503 → MFS transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_002947593,4330GT11.3% 138.2 / 7.7 51E391* (GAG→TAG) ‡PP_0503MFS transporter
Reads supporting (aligned to +/- strand):  ref base G (24/20);  new base T (3/3);  total (28/23)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.56e-01

ATTCGCTGGGCAGCGGTTTGGGGGCGGTGTTGGCGAGCTGGGTGTTTGCACACTGGGGCTGGGTTGCGGTGTGTGGGCTTGGCATGGGCATCAGTGCCGCCGCACTGTGCTACTGGCTATGGCTGCAACGCGCGAGGGCGGCCGAGGCCGCCCTGCAATGTTCAGGTCAGAACTTGTAGCCAAGACCGACCATGTATACCCACGGGTCGACGTCGACGTCGACCTTGGTCTTGCTGTAGCCAAGGGCGGTCGGGCCGTTGACGCTGGCCTTGGTGTCGATGTCGACGTAC  >  NC_002947/593290‑593579
                                                                                                                                               |                                                                                                                                                  
atacGCTGGGCAGCGGTTTGGGGGCGGTGTTGGCGAGCTGGGTGTTTGCACACTGGGGCTGGGATGCGGTGTGTGGGCTTGGCATGGGCATCAGTGCCGCCGCACTGTGCTACTGGCTATGGCTGCAACGCGCGAGGGCGCCCCAGgcc                                                                                                                                               >  3:255176/4‑149 (MQ=255)
 ttCGCTGGGCAGCGGTTTGGGGGCGGTGTTGGCGAGCTGGGTGTTTGCACACTGGGGCTGGGTTGCGGTGTGTGGGCTTGGCATGGGCATCAGTGCCGCCGCACTGTGCTACTGGCTATGGCTGCAACGCGCGAGGGCGGCCGAGgccg                                                                                                                                              <  3:341749/149‑1 (MQ=255)
    gCTGGGCAGCGGTTTGGGGGCGGTGTTGGCGAGCTGGGTGTTTGCACACTGGGGCTGGGTTGCGGTGTGTGGGCTTGGCATGGGCATCAGTGCCGCCGCACTGTGCTACTGGCTATGGCTGCAACGCGCGAGGGCGGCCGAGGCCGccc                                                                                                                                           >  8:2126/1‑149 (MQ=255)
        ggCAGCGGTTTGGGGGCGGTGTTGGCGAGCTGGGTGTTTGCACACTGGGGCTGGGTTGCGGTGTGTGGGCTTGGCATGGGCATCAGTGCCGCCGCACTGTGCTACTGGCTATGGCTGCAACGCGCGAGGGCGGCCTCGGCCGCCCtcg                                                                                                                                        <  6:39431/148‑3 (MQ=255)
              ggTTTGGTGGCGGTGTTGGCGAGCTGGGTGTTTGCACACTGGGGCTGGGTTGCGGTGTGTGCGCTTGGCATGGGCATCAGTGCCGCCGCACTGTGCTACTGGCTATGGCTGCAACGCGCGTGGGCGGCCGAGGCCGCCCTGCAATGTTc                                                                                                                                 <  7:179711/149‑1 (MQ=255)
                            gtTGGCGAGCTGGGTGTTTGCACACTGGGGCTGGGTTGCGGTGTGTGGGCTTGGCATGGGCATCAGTGCCGCCGCACTGTGCTACTGGCTATGGCTGCAACGCGCGAGGGCGGCCGAGGCCGCCCTGc                                                                                                                                        >  8:12569/1‑128 (MQ=255)
                            gtTGGCGAGCTGGGTGTTTGCACACTGGGGCTGGGTTGCGGTGTGTGGGCTTGGCATGGGCATCAGTGCCGCCGCACTGTGCTACTGGCTATGGCTGCAACGCGCGAGGGCGGCCGAGGCCGCCCTGc                                                                                                                                        <  7:12569/128‑1 (MQ=255)
                                  gAGCTGGTGGTTTGGACACTGGGGCTGGGTTGCGGTGTGTGGGCTTGGCATGGGCATCAGTGCCGCCGCACTGTGCTACTGGCTATGGCTGCAACGCGCGAGGGCGGCCGAGGCCGCCCTGCAATGTTCAGGTCAGAACTTGTAGCCaa                                                                                                             <  4:255176/149‑1 (MQ=255)
                                      tGGGTGTTTGCACACTGGGGCTGGGTTGCGGTGTGTGGGCTTGGCATGGGCATCAGTGCCGCCGCACTGTGCTACTGGCTATGGCTGCAACGCGCGAGGGCGGCCTCGGCCGCCCt                                                                                                                                          >  1:71586/1‑116 (MQ=255)
                                      tGGGTGTTTGCACACTGGGGCTGGGTTGCGGTGTGTGGGCTTGGCATGGGCATCAGTGCCGCCGCACTGTGCTACTGGCTATGGCTGCAACGCGCGAGGGCGGCCTCGGCCGCCCt                                                                                                                                          <  2:71586/116‑1 (MQ=255)
                                      tGGGTGTTTGCACACTGGGGCTGGGTTGCGGTGTGTGGGCTTGGCATGGGCATCAGTGCCGCCGCACTGTGCTACTGGCTATGGCTGCAACGCGCGAGGGCGGCCGAGGCCGCCCTGCAATGTTCAg                                                                                                                               <  1:74572/127‑1 (MQ=255)
                                      tGGGTGTTTGCACACTGGGGCTGGGTTGCGGTGTGTGGGCTTGGCATGGGCATCAGTGCCGCCGCACTGTGCTACTGGCTATGGCTGCAACGCGCGAGGGCGGCCGAGGCCGCCCTGCAATGTTCAg                                                                                                                               >  2:74572/1‑127 (MQ=255)
                                         gtgtTTGCACACTGGGGCTGGGTTGCGGTGTGTGGGCTTGGCATGGGCATCAGTGCCGCCGCACTGTGCTACTGGCTATGGCTGCAACGCGCGAGGGCGGCCGAGGCCGCCCTGCAATGTTCAGGt                                                                                                                             <  6:234845/126‑1 (MQ=255)
                                         gtgtTTGCACACTGGGGCTGGGTTGCGGTGTGTGGGCTTGGCATGGGCATCAGTGCCGCCGCACTGTGCTACTGGCTATGGCTGCAACGCGCGAGGGCGGCCGAGGCCGCCCTGCAATGTTCAGGt                                                                                                                             >  5:239910/1‑126 (MQ=255)
                                         gtgtTTGCACACTGGGGCTGGGTTGCGGTGTGTGGGCTTGGCATGGGCATCAGTGCCGCCGCACTGTGCTACTGGCTATGGCTGCAACGCGCGAGGGCGGCCGAGGCCGCCCTGCAATGTTCAGGt                                                                                                                             >  5:234845/1‑126 (MQ=255)
                                         gtgtTTGCACACTGGGGCTGGGTTGCGGTGTGTGGGCTTGGCATGGGCATCAGTGCCGCCGCACTGTGCTACTGGCTATGGCTGCAACGCGCGAGGGCGGCCGAGGCCGCCCTGCAATGTTCAGGt                                                                                                                             <  6:239910/126‑1 (MQ=255)
                                             ttGCACACTGGGGCTGGGTTGCGGTGTGTGGGCTTGGCATGGGCATCAGTGCCGCCGCACTGTGCTACTGGCTATGGCTGCAACGCGCGAGGGCGGCCGAGGCCGCCCTGCAATGTTCAGGTCAGAACTTGTAGCCAAGACCGACCATg                                                                                                  >  5:39590/1‑149 (MQ=255)
                                             ttGCACACTGGGGCTGGGTTGCGGTGTGTGGGCTTGGCATGGGCATCAGTGCCGCCGCACTGTGCTACTGGCTATGGCTGCAACGCGCGAGGGCGGCCGAGGCCGCCCTGCAATGTTCAGGTCAGAACTTGTAGCCAAGACCGACCATg                                                                                                  >  5:34375/1‑149 (MQ=255)
                                                 acacTGGGGCTGGGTTGCGGTGTGTGGGCTTGGCATGGGCATCAGTGCCGCCGCACTGTGCTACTGGCTATGGCTGCAACGCGCGAGGGCGGCCGAGGCCGCCCTGCAATGTTCAGGTCAGAACTTGTAGCCAAGACCGACCATGtata                                                                                              <  6:39590/149‑1 (MQ=255)
                                                 acacTGGGGCTGGGTTGCGGTGTGTGGGCTTGGCATGGGCATCAGTGCCGCCGCACTGTGCTACTGGCTATGGCTGCAACGCGCGAGGGCGGCCGAGGCCGCCCTGCAATGTTCAGGTCAGAACTTGTAGCCAAGACCGACCATGtata                                                                                              <  6:34375/149‑1 (MQ=255)
                                                   acTGGGGCTGGGTTGCGGTGTGTGGGCTTGGCATGGGCATCAGTGCCGCCGCACTGTGCTACTGGCTATGGCTGCAACGCGCGAGGGCGGCCGAGGCCGCCCTGCAATGTTCAGGTCAGAACtt                                                                                                                     <  3:98717/124‑1 (MQ=255)
                                                   acTGGGGCTGGGTTGCGGTGTGTGGGCTTGGCATGGGCATCAGTGCCGCCGCACTGTGCTACTGGCTATGGCTGCAACGCGCGAGGGCGGCCGAGGCCGCCCTGCAATGTTCAGGTCAGAACtt                                                                                                                     >  4:98717/1‑124 (MQ=255)
                                                   acTGGGGCTGGGTTGCGGTGTGTGGGCTTGGCATGGGCATCAGTGCCGCCGCACTGTGCTACTGGCTATGGCTGCAACGCGCGAGGGCGGCCGAGGCCGCCCTGCAATGTTCAGGTCAGAACTTGTAGCCAAGACCGACCATGTATAcc                                                                                            >  3:16854/1‑149 (MQ=255)
                                                                       tgtgGGCTTGGCATGGGCATCAGTGCCGCCGCACTGTGCTACTGGCTATGGCTGCAACGCGCGAGGGCGGCCGAGGCCGCCCTGCAATGTTCAGGTCAGAACTTGTAGCCAAgaccgac                                                                                                      <  6:93813/119‑1 (MQ=255)
                                                                       tgtgGGCTTGGCATGGGCATCAGTGCCGCCGCACTGTGCTACTGGCTATGGCTGCAACGCGCGAGGGCGGCCGAGGCCGCCCTGCAATGTTCAGGTCAGAACTTGTAGCCAAgaccgac                                                                                                      >  5:93813/1‑119 (MQ=255)
                                                                              ttGGCATGGGCATCAGTGCCGCCGCACTGTGCTACTGGCTATGGCTGCAACGCGCGAGGGCGGCCGAGGCCGCCCTGCAATGTTCAGGTCAGAACTTGTAGCCAAGACCGACCATGTATACCCACGGGTCGACGTCGACGTCGACCTTg                                                                 <  4:16854/149‑1 (MQ=255)
                                                                                 gCATGGGCATCAGTGCCGCCGCACTGTGCTACTGGCTATGGCTGCAACGCGCGAGGGCGGCCGAGGCCGCCCTGCAATg                                                                                                                                    <  2:220455/79‑1 (MQ=255)
                                                                                 gCATGGGCATCAGTGCCGCCGCACTGTGCTACTGGCTATGGCTGCAACGCGCGAGGGCGGCCGAGGCCGCCCTGCAATg                                                                                                                                    >  1:220455/1‑79 (MQ=255)
                                                                                    tGGGCATCAGTGCCGCCGCACTGTGCTACTGGCTATGGCTGCAACGCGCGAGGGCGGCCGAGGCCGCCCTGCAATGTTCAGGTCAGAACTTGTAGCCAAGACCGACCATGTATACCCACGGGTCGACGTCGACGTCGACCTTGGTCTTg                                                           >  4:37235/1‑149 (MQ=255)
                                                                                       gCATCAGTGCCGCCGCACTGTGCTACTGGCTATGGCTGCAACGCGCGAGGGCGGCCGAGGCCGCCCTGCAATGTTCAGGTCAGAACTTGTAGCCAAGACCGACCATGTATACCCACGGGTCGACGTCGACGTCGACCTTGGTCTTGCTg                                                        >  3:174993/1‑149 (MQ=255)
                                                                                       gCATCAGTGCCGCCGCACTGTGCTACTGGCTATGGCTGCAACGCGCGAGGGCGGCCGAGGCCGCCCTGCAATGTTCAGGTCAGAACTTGTAGCCAAGACCGACCATGTATACCCACGGGTCGACGTCGACGTCGACCTTGGTCTTGCTg                                                        >  8:317186/1‑149 (MQ=255)
                                                                                        gcacAGTGCGGCGGCACTGTGCTACTGGCTATGGCTGCAACGCGCGAGGGCGGCCGAGGCCGCCCTGCAATGTTCAGGTCAGAACTTGTAGCCAAGACCGACCATGTATACCCACGGGTCGACGTCGACGTCGACCTTGGTCTTGCTg                                                        >  2:39004/4‑148 (MQ=255)
                                                                                                         tgtgCTACTGGCTATGGCTGCAACGCGCGAGGGCGGCCGAGGCCGCCCTGCAATGTTCAGGTCAGAACTTGTAGCCAAGACCGACCATGTATACCCACGGGTCGACGTCGACGTCGACCTTGGTCTTGCTGTAGCCAAGGGCGGTCggg                                      >  1:131712/1‑149 (MQ=255)
                                                                                                           tgCTACTGGCTATGGCTGCAACGCGCGAGGGCGGCCGAGGCCGCCCTGCAATGTTCAGGTCAGAACTTGTAGCCAAGACCGACCATGTATACCCACGGGTCGACGTCGACGTCGACCTTGGTCTTGCTGTAGCCAAGGGCGGTCggg                                      <  4:174993/147‑1 (MQ=255)
                                                                                                              tACTGGCTATGGCTGCAACGCGCGAGGGCGGCCGAGGCCGCCCTGCAATGTTCAGGTCAGAACTTGTAGCCAAGACCGACCATGTATACCCACGGGTCGACGTCGACGTCGACCTTGGTCTTGCTGTAGCCaa                                                 >  7:318824/1‑133 (MQ=255)
                                                                                                              tACTGGCTATGGCTGCAACGCGCGAGGGCGGCCGAGGCCGCCCTGCAATGTTCAGGTCAGAACTTGTAGCCAAGACCGACCATGTATACCCACGGGTCGACGTCGACGTCGACCTTGGTCTTGCTGTAGCCaa                                                 <  8:318824/133‑1 (MQ=255)
                                                                                                                cTGGCTATGGCTGCAACGCGCGAGGGCGGCCGAGGCCGCCCTGCAATGTTCAGGTCAGAACTTGTAGCCAAGACCGACCATGTATACCCACGGGTCGACGTCGACGTCGACCTTGGTCTTGCTGTAGCCAAGGGCGGTCGGGCCGTTGa                               >  5:212087/1‑149 (MQ=255)
                                                                                                                cTGGCTATGGCTGCAACGCGCGAGGGCGGCCGAGGCCGCCCTGCAATGTTCAGGTCAGAACTTGTAGCCAAGACCGACCATGTATACCCACGGGTCGACGTCGACGTCGACCTTGGTCTTGCTGTAGCCAAGGGCGGTCGGGCCGTTGa                               <  7:317186/149‑1 (MQ=255)
                                                                                                                   gCTATGGCTGCAACGCGCGAGGGCGGCCGAGGCCGCCCTGCAATGTTCAGGTCAGAACTTGTAGCCAAGACCGACCATGTATACCCACGGGTCGACGTCGACGTCGACCTTGGTCTTGCTGTAGCCAAGGGCGGTCGGGCCGTTGACGc                            >  2:230256/1‑149 (MQ=255)
                                                                                                                                  ttgcaGGGCGGCCTCGGCCGCCCTGCAATGTTCAGGTCAGAACTTGTAGCCAAGACCGACCATGTATACCCACGGGTCGACGTCGACGTCGACCTTGGTCTTGCTGTAGCCAAGGGCGGTCGGGCCGTTGACGCTGGCCTTGGtgtcga             >  7:270361/5‑149 (MQ=255)
                                                                                                                                  ttgcaGGGCGGCCTCGGCCGCCCTGCAATGTTCAGGTCAGAACTTGTAGCCAAGACCGACCATGTATACCCACGGGTCGACGTCGACGTCGACCTTGGTCTTGCTGTAGCCAAGGGCGGTCGGGCCGTTGACGCTGGCCTTGGtgtcga             >  1:65220/5‑149 (MQ=255)
                                                                                                                                  cgcgAGGGCGGCCGAGGCCGCCCTGCAATGTTCAGGTCAGAACTTGTAGCCAAGACCGACCATGTATACCCACGGGTCGACGTCGACGTCGACCTTGGTCTTGCTGTAGCCAAGGGCGGTCGGGCCGTTGACGCTGGCCTTGGtgtcga             <  2:131712/149‑1 (MQ=255)
                                                                                                                                   gcgAGGGCGGCCGAGGCCGCCCTGCAATGTTCAGGTCAGAACTTGTAGCCAAGACCGACCATGTATACCCACGGGTCGACGTCGACGTCGACCTTGGTCTTGCTGTAGCCAAGGGCGGTCGGGCCGTTGACGCTGGCCTTGGtgtcgat            <  3:37235/149‑1 (MQ=255)
                                                                                                                                   gcgAGGGCGGCCGAGGCCGCCCTGCAATGTTCAGGTCAGAACTTGTAGCCAAGACCGACCATGTATACCCACGGGTCGACGTCGACGTCGACCTTGGTCTTGCTGTAGCCAAGGGCGGTCGGGCCGTTGACGCTGGCCTTGGtgtcgat            >  2:140600/1‑149 (MQ=255)
                                                                                                                                   gcgAGGGCGGCCGAGGCCGCCCTGCAATGTTCAGGTCAGAACTTGTAGCCAAGACCGACCATGTATACCCACGGGTCGACGTCGACGTCGACCTTGGTCTTGCTGTAGCCAAGGGCGGTCGGGCCGTTGACGCTGGCCTTGGtgtcgat            >  1:188426/1‑149 (MQ=255)
                                                                                                                                     gAGGGCGGCCGAGGCCGCCCTGCAATGTTCAGGTCAGAACTTGTAGCCAAGACCGACCATGTATACCCACGGGTCGACGTCGACGTCGACCTTGGTCTTGCTGTAGCCAAGGGCGGTCGGGCCGTTGACGCt                           <  6:14741/132‑1 (MQ=255)
                                                                                                                                     gAGGGCGGCCGAGGCCGCCCTGCAATGTTCAGGTCAGAACTTGTAGCCAAGACCGACCATGTATACCCACGGGTCGACGTCGACGTCGACCTTGGTCTTGCTGTAGCCAAGGGCGGTCGGGCCGTTGACGCt                           >  5:14741/1‑132 (MQ=255)
                                                                                                                                     gAGGGCGGCCGAGGCCGCCCTGCAATGTTCAGGTCAGAACTTGTAGCCAAGACCGACCATGTATACCCACGGGTCGACGTCGACGTCGACCTTGGTCTTGCTGTAGCCAAGGGCGGTCGGGCCGTTGACGCTGGCCTTGGtgtcgatgt          >  2:210901/1‑149 (MQ=255)
                                                                                                                                         gcggcCTCGGCCGCCCTGCAATGTTCAGGTCAGAACTTGTAGCCAAGACCGACCATGTATACCCACGGGTCGACGTCGACGTCGACCTTGGTCTTGCTGTAGCCAAGGGCGGTCGGGCCGTTGACGCTGGCCTTGGTGTCGATGTCGAc      <  2:65220/149‑1 (MQ=255)
                                                                                                                                          cggcCGAGGCCGCCCTGCAATGTTCAGGTCAGAACTTGTAGCCAAGACCGACCATGTATACCCACGGGTCGACGTCGACGTCGACCTTGGTCTTGCTGTAGCCAAGGGCGGTCGGGCCGTTGACGCTGGCCTTGGTGTCGATGTCGACg     <  2:188426/149‑1 (MQ=255)
                                                                                                                                              cGAGGCCGCCCTGCAATGTTCAGGTCAGAACTTGTAGCCAAGACCGACCATCTATACCCACGGGTCGACGTCGACGTCGACCTTGGTCTTGCTGTAGCCAAGGGCGGTCGGGCCGTTCACGCTGGCCTAGGTGTCGATGTCGACGTaa  >  4:200590/1‑147 (MQ=255)
                                                                                                                                               |                                                                                                                                                  
ATTCGCTGGGCAGCGGTTTGGGGGCGGTGTTGGCGAGCTGGGTGTTTGCACACTGGGGCTGGGTTGCGGTGTGTGGGCTTGGCATGGGCATCAGTGCCGCCGCACTGTGCTACTGGCTATGGCTGCAACGCGCGAGGGCGGCCGAGGCCGCCCTGCAATGTTCAGGTCAGAACTTGTAGCCAAGACCGACCATGTATACCCACGGGTCGACGTCGACGTCGACCTTGGTCTTGCTGTAGCCAAGGGCGGTCGGGCCGTTGACGCTGGCCTTGGTGTCGATGTCGACGTAC  >  NC_002947/593290‑593579

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑