Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 629,375 A→G 5.6% S275P (TCG→CCG)  eutB ← ethanolamine ammonia‑lyase subunit alpha

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_002947629,3750AG 5.6% 210.5 / 2.1 71S275P (TCG→CCG) eutBethanolamine ammonia‑lyase subunit alpha
Reads supporting (aligned to +/- strand):  ref base A (35/32);  new base G (2/2);  total (37/34)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.85e-01

GGAACGGCTTGAAGTGACGAGCCACGGCATAGGCGCGGGTTTCGCAGGTCTGCTGGTCGACGCCGTGGTGGGCGTTGGCCGAGAGCGCGCTGCCCTGGCCGGTTTCGAAATACATCAGGTTCTGCCCGAGGGTGCCACGTTTCAGCGACAGCCCTGCTTCGTAGCCTTCCTGCAACACGTTCAGGTTGATGCCGAAGCCGGCGTTGGCCGCCTCTGTGCCGGCGATGGACTGGAACACCAGGTCCAGTGGCACGCCGCGGTTGATCGCTTCGATCGAGGTGGTGACGTGGGTCAGC  >  NC_002947/629228‑629523
                                                                                                                                                   |                                                                                                                                                    
ggAACGGCTTGAAGTGACGAGCCACGGCATAGGCGCGGGTTTCGCAGGTCTGCTGGTCGACGCCGTGGTGGGCGTTGGCCGAGAGCGCGCTGCCCTGGCCGGTTTCGAAATACATCAGGTTCTGCCCGAGGGTGCCACGTTTCAGCGa                                                                                                                                                      >  6:358805/1‑148 (MQ=255)
   aCGGCTTGAAGTGACGAGCCACGGCATAGGCGCGGGTTTCGCAGGTCTGCTGGTCGACGCCGTGGTGGGCGTTGGCCGAGAGCGCGCTGCCCTGGCCGGTTTCGAAATACATCAGGTTCTGCCCGAGGGTGCCACGTTTCAGCGACAGc                                                                                                                                                  >  6:325259/1‑149 (MQ=255)
             gTGACGAGCCACGGCATAGGCGCGGGTTTCGCAGGTCTGCTGGTCGACGCCGTGGTGGGCGTTGGCCGAGAGCGCGCTGCCCTGGCCGGTTTCGAAATACATCAGGTTCTGCCCGAGGGTGCCACGTTTCAGCGACAGCCCTGCTTCGt                                                                                                                                        <  7:325792/149‑1 (MQ=255)
              tGACGAGCCACGGCATAGGCGCGGGTTTCGCAGGTCTGCTGGTCGACGCCGTGGTGGGCGTTGGCCGAGAGCGCGCTGCCCTGGCCGGTTTCGAAATACATCAGGTTCTGCCCGAGGGTGCCACGTTTCAGCGACAGCCCTGCTTCGTa                                                                                                                                       <  5:325259/149‑1 (MQ=255)
              tGACGAGCCACGGCATAGGCGCGGGTTTCGCAGGTCTGCTGGTCGACGCCGTGGTGGGCGTTGGCCGAGAGCGCGCTGCCCTGGCCGGTTTCGAAATACATCAGGTTCTGCCCGAGGGTGCCACGTTTCAGCGACAGCCCTGCTTCGTa                                                                                                                                       <  3:294623/149‑1 (MQ=255)
               gACGAGCCACGGCATAGGCGCGGGTTTAGCAGGTCTGCTGGTCGACGCCGTGGTGGGCGTTGGCCGCGAGCGCGCTGCCCTGGCCGGTTTCGAAAAACATCAGGTTCTGCCCGAGGGTGCCACGTTTCAGCGACAGCCCTGCTTCGTAg                                                                                                                                      >  6:223512/1‑149 (MQ=255)
                    gCCACGGCATAGGCGCGGGTTTCGCAGGTCTGCTGGTCGACGCCGTGGTGGGCGTTGGCCGAGAGCGCGCTGCCCTGGCCGGTTTCGAAATACATCAGGTTCTGCCCGAGGGTGCCACGTTTCAGCGACAGCCCTGCTTCGTAGCCTTc                                                                                                                                 <  3:281571/149‑1 (MQ=255)
                       aCGGCATAGGCGCGGGTTTCGCAGGTCTGCTGGTCGACGCCGTGGTGGGCGTTGGCCGAGAGCGCGCTGCCCTGGCCGGTTTCGAAATACATCAGGTTCTGCCCGAGGGTGCCACGTTTCAGCGACAGCCCTGCTTCGTAGCCTTCCTg                                                                                                                              <  8:129068/149‑1 (MQ=255)
                         ggCATAGGCGCGGGTTTCGCAGGTCTGCTGTTCGACGCCGTGGTGGGCGTTGGCCGAGAGCGCGCTGCCCTGGCCGGTTTCGAAATACATCAGGTTCTGCCCGAGGGTGCCACGTTTCAGCGACAGCCCt                                                                                                                                               <  6:327364/130‑1 (MQ=255)
                         ggCATAGGCGCGGGTTTCGCAGGTCTGCTGGTCGACGCCGTGGTGGGCGTTGGCCGAGAGCGCGCTGCCCTGGCCGGTTTCGAAATACATCAGGTTCTGCCCGAGGGTGCCACGTTTCAGCGACAGCCCt                                                                                                                                               >  5:327364/1‑130 (MQ=255)
                                  gcgGGTTTCGCAGGTCTGCTGGTCGACGCCGTGGTGGGCGTTGGCCGAGAGCGCGCTGCCCTGGCCGGTTTCGAAATACATCAGGTTCTGCCCGAGGGTGCCACGTTTCAGCGGCAGCCCTGCTTCGTAGCCTTCCTGCAACACGtt                                                                                                                     >  6:25283/1‑147 (MQ=255)
                                  gcgGGTTTCGCAGGTCTGCTGGTCGACGCCGTGGTGGGCGTTGGCCGAGAGCGCGCTGCCCTGGCCGGTTTCGAAATACATCAGGTTCTGCCCGAGGGTGCCACGTTTCAGCGGCAGCCCTGCTTCGTAGCCTTCCTGCAACACGtt                                                                                                                     >  5:129417/1‑147 (MQ=255)
                                  gcgGGTTTCGCAGGTCTGCTGGTCGACGCCGTGGTGGGCGTTGGCCGAGAGCGCGCTGCCCTGGCCGGTTTCGAAATACATCAGGTTCTGCCCGAGGGTGCCACGTTTCAGCGACAGCCCTGCTTCGTAGCCTTCCTGc                                                                                                                             >  7:192401/1‑139 (MQ=255)
                                  gcgGGTTTCGCAGGTCTGCTGGTCGACGCCGTGGTGGGCGTTGGCCGAGAGCGCGCTGCCCTGGCCGGTTTCGAAATACATCAGGTTCTGCCCGAGGGTGCCACGTTTCAGCGACAGCCCTGCTTCGTAGCCTTCCTGc                                                                                                                             <  8:192401/139‑1 (MQ=255)
                                               gTCTGCTGGTCGACGCCGTGGTGGGCGTTGGCCGAGAGCGCGCTGCCCTGGCCGGTTTCGAAATACATCAGGTTCTGCCCGAGGGTGCCACGTTTCAGCGACAGCCCTGCTTCGTAGCCTTCCTGCAACACGTTCTGGTTGATGCCg                                                                                                        >  1:139416/1‑147 (MQ=255)
                                                   gctgGTCGACGCCGTGGTGGGCGTTGGCCGAGAGCGCGCTGCCCTGGCCGGTTTCGAAATACATCAGGTTCTGCCCGAGGGTGCCACGTTTCAGCGACAGCCCTGCTTCGTAGCCTTCCTGCAACACGTTCAGGTTGATGCCGAAGCCg                                                                                                  >  5:72448/1‑149 (MQ=255)
                                                    ctgGTCGACGCCGTGGTGGGCGTTGGCCGAGAGCGCGCTGCCCTGGCCGGTTTCGAAATACATCAGGTTCTGCCCGAGGGTGCCACGTTTCAGCGACAGCCCTGCTTCGTAGCCTTCCTGCAACACGTTCAGGTTGATGCCGAAGCCgg                                                                                                 >  3:187806/1‑149 (MQ=255)
                                                     tgGTCGACGCCGTGGTGGGCGTTGGCCGAGAGCGCGCTGCCCTGGCCGGTTTCGAAATACATCAGGTTCTGCCCGAGGGTGCCACGTTTCAGCGACAGCCCTGCTTCGTAGCCTTCCTGCAACACGTTCa                                                                                                                   >  7:30036/1‑130 (MQ=255)
                                                     tgGTCGACGCCGTGGTGGGCGTTGGCCGAGAGCGCGCTGCCCTGGCCGGTTTCGAAATACATCAGGTTCTGCCCGAGGGTGCCACGTTTCAGCGACAGCCCTGCTTCGTAGCCTTCCTGCAACACGTTCa                                                                                                                   <  8:30036/130‑1 (MQ=255)
                                                       gTCGACGCCGTGGTGGGCGTTGGCCGAGAGCGCGCTGCCCTGGCCGGTTTCGAAATACATCAGGTTCTGCCCGAGGGTGCCACGTTTCAGCGACAGCCCTGCTTCGTAGCCTTCCTGCAACACGTTCAGGTTGATGCCGAAGCCGGCGt                                                                                              >  5:119135/1‑149 (MQ=255)
                                                          gACGCCGTGGTGGGCGTTGGCCGAGAGCGCGCTGCCCTGGCCGGTTTCGAAATACATCAGGTTCTGCCCGAGGGTGCCACGTTTCAGCGACAGCCCTGCTTCGTAGCCTTCCTGCAACACGTTCAg                                                                                                                  >  6:117325/1‑126 (MQ=255)
                                                          gACGCCGTGGTGGGCGTTGGCCGAGAGCGCGCTGCCCTGGCCGGTTTCGAAATACATCAGGTTCTGCCCGAGGGTGCCACGTTTCAGCGACAGCCCTGCTTCGTAGCCTTCCTGCAACACGTTCAg                                                                                                                  <  5:117325/126‑1 (MQ=255)
                                                          gACGCCGTGGTGGGCGTTGGCCGAGAGCGCGCTGCCCTGGCCGGTTTCGAAATACATCAGGTTCTGCCCGAGGGTGCCACGTTTCAGCGACAGCCCTGCTTCGTAGCCTTCCTGCAACACGTTCAGGTTGATGCCGAAGCCGGCGTTgg                                                                                           >  3:219345/1‑149 (MQ=255)
                                                           aCGCCGTGGTGGGCGTTGGCCGAGAGCGCGCTGCCCTGGCCGGTTTCGAAATACATCAGGTTCTGCCCGAGGGTGCCACGTTTCAGCGGCAGCCCTGCTTCGTAGCCTTCCTGCAACACGTTCAGGTTGATGCCGAAGCCGGCGTTGgc                                                                                          <  5:25283/149‑1 (MQ=255)
                                                           aCGCCGTGGTGGGCGTTGGCCGAGAGCGCGCTGCCCTGGCCGGTTTCGAAATACATCAGGTTCTGCCCGAGGGTGCCACGTTTCAGCGGCAGCCCTGCTTCGTAGCCTTCCTGCAACACGTTCAGGTTGATGCCGAAGCCGGCGTTGgc                                                                                          <  6:129417/149‑1 (MQ=255)
                                                                    tggGCGTTGGCCGAGAGCGCGCTGCCCTGGCCGGTTTCGAAATACATCAGGTTCTGCCCGAGGGTGCCACGTTTCAGCGACAGCCCTGCTTCGTAGCCTTCCTGCAACACGTTCAGGTTGAt                                                                                                            <  6:296207/122‑1 (MQ=255)
                                                                    tggGCGTTGGCCGAGAGCGCGCTGCCCTGGCCGGTTTCGAAATACATCAGGTTCTGCCCGAGGGTGCCACGTTTCAGCGACAGCCCTGCTTCGTAGCCTTCCTGCAACACGTTCAGGTTGATGCCGAAGCCGGCGTTGGCCGCCTCtgt                                                                                 <  6:72448/149‑1 (MQ=255)
                                                                       gCGTTGGCCGAGAGCGCGCTGCCCTGGCCGGTTTCGAAATACATCAGGTTCTGCCCGAGGGTGCCACGTTTCAGCGACAGCCCTGCTTCGTAGCCTTCCTGCAACACGTTCAGGTTGATGCCGAAGCCGGCGTTGGCCGCctct                                                                                   >  1:333294/1‑144 (MQ=255)
                                                                       gCGTTGGCCGAGAGCGCGCTGCCCTGGCCGGTTTCGAAATACATCAGGTTCTGCCCGAGGGTGCCACGTTTCAGCGACAGCCCTGCTTCGTAGCCTTCCTGCAACACGTTCAGGTTGATGCCGAAGCCGGCGTTGGCCGCctct                                                                                   <  2:333294/144‑1 (MQ=255)
                                                                             gCCGAGAGCGCGCTGCCCTGGCCGGTTTCGAAATACATCAGGTTCTGCCCGAGGGTGCCACGTTTCAGCGACAGCCCTGCTTCGTAGCCTTCCTGCAACACGTTCAGGTTGATGCCGAAGCCGGCGTTGGCCGCCTCTGTGCCGGCGAt                                                                        <  4:219345/149‑1 (MQ=255)
                                                                                 agagCGCGCTGCCCTGGCCGCTTTCGAAATACATCAGGTTCTGCCCGAGGGTGCCACGGTTCAGCGACAGCCCTGCATCGTAGCCTTCCTGCAACACGTTCAGGTTGATGCCGAAGCCGCCGATGGCCGCCTCTGTGCCGGCGATGGAc                                                                    >  5:364423/1‑149 (MQ=255)
                                                                                         cTGCCCTGGCCGGTTTCGAAATACATCAGGTTCTGCCCGAGGGTGCCACGTTTCAGCGACAGCCCTGCTTCGTAGCCTTCCTGCAACACGTTCAGGTTGATGCCGAAGCCGGCGTTGGCCGCCTCTGTGCCGGCGATGGACTGGAacac                                                            <  5:223512/149‑1 (MQ=255)
                                                                                         cTGCCCTGGCCGGTTTCGAAATACATCAGGTTCTGCCCGAGGGTGCCACGTTTCAGCGACAGCCCTGCTTCGTAGCCTTCCTGCAACACGTTCAGGTTGATGCCGAAGCCGGCGTTGGCCGCCTCTGTGCCGGCGATGGACTGGAacac                                                            <  5:358805/149‑1 (MQ=255)
                                                                                             ccTGGCCGGTTTCGAAATACATCAGGTTCTGCCCGAGGGTGCCACGTTTCAGCGACAGCCCTGCTTCGTAGCCTTCCTGCAACACGTTCAGGTTGATGCCGAAGCCGGCGTTGGCCGCCTCTGTGCCGGCGATGGACTGGAACACCAgg                                                        <  2:232088/149‑1 (MQ=255)
                                                                                                   cGGTTTCGAAATACATCAGGTTCTGCCCGAGGGTGCCACGTTTCAGCGACAGCCCTGCTTCGTAGCCTTCCTGCAACACGTTCAGGTTGATGCCGAAGCCGGCGTTGGCCGCCTCTGTGCCGGCGATGGACTGGAACACCAGGTCCAg                                                   <  4:295976/148‑1 (MQ=255)
                                                                                                   cGGTTTCGAAATACATCAGGTTCTGCCCGAGGGTGCCACGTTTCAGCGACAGCCCTGCTTCGTAGCCTTCCTGCAACACGTTCAGGTTGATGCCGAAGCCGGCGTTGGCCGCCTCTGTGCCGGCGATGGACTGGAACACCAGGTCCAg                                                   >  3:295976/1‑148 (MQ=255)
                                                                                                    ggTTTCGAAATACATCAGGTTCTGCCCGAGGGTGCCACGTTTCAGCGACAGCCCTGCTTCGTAGCCTTCCTGCAACACGTTCAGGTTGATGCCGAAGCCGGCGTTGGCCGCCTCTGTGCCGGCGATGGACTGGAACACCAGGTCCAGTg                                                 <  4:187806/149‑1 (MQ=255)
                                                                                                     gTTTCGAAATACATCAGGTTCTGCCCGAGGCTGCCACGTTTCAGCGACAGCCCTGCTTCGTAGCCTTCCTGCAACACGTTCAGGTTGATGCCGAAGCCGGCGTTGGCCGCCTCTGTGCCGGCGATGGACTGGAACACCAGGTCCAGTgg                                                >  1:30275/1‑149 (MQ=255)
                                                                                                      tttCGAAATACATCAGGTTCTGCCCGAGGGTGCCACGTTTCAGCGACAGCCCTGCTTCGTAGCCTTCCTGCAACACGTTCAGGTTGATg                                                                                                           <  8:62392/89‑1 (MQ=255)
                                                                                                      tttCGAAATACATCAGGTTCTGCCCGAGGGTGCCACGTTTCAGCGACAGCCCTGCTTCGTAGCCTTCCTGCAACACGTTCAGGTTGATg                                                                                                           >  7:62392/1‑89 (MQ=255)
                                                                                                       ttCGAAATACATCAGGTTCTGCCCGAGGGTGCCACGTTTCAGCGACAGCCCTGCTTCGTAGCCTTCCTGCAACACGTTCAGGTTGATGCCGAAGCCGGCGTTGGCCGCCTCTGTGCCGGCGATGGACTGGAACACCAGGTCCAGTGGCa                                              >  3:261764/1‑149 (MQ=255)
                                                                                                         cGAAATACATCAGGTTCTGCCCGAGGGTGCCACGTTTCAGCGACAGCCCTGCTTCGTAGCCTTCCTGCAACACGTTCAGGTTGATGCCGAAGCCGGCGTTGGCCGCCTCTGTGCCGGCGATGGACTGGAACACCAGGTCCAGTGGCAcg                                            >  5:150441/1‑149 (MQ=255)
                                                                                                                 aTCAGGTTCTGCCCGAGGGTGCCACGTTTCAGCGACAGCCCTGCTTCGTAGCCTTCCTGCAACACGTTCAGGTTGATg                                                                                                           >  5:155737/1‑78 (MQ=255)
                                                                                                                 aTCAGGTTCTGCCCGAGGGTGCCACGTTTCAGCGACAGCCCTGCTTCGTAGCCTTCCTGCAACACGTTCAGGTTGATg                                                                                                           <  6:155737/78‑1 (MQ=255)
                                                                                                                 aTCAGGTTCTGCCCGAGGGTGCCACGTTTCAGCGACAGCCCTGCTTCGTAGCCTTCCTGCAACACGTTCAGGTTGATGCCGAAGCCGGCGTTGGCCGCCTCTGTGCCGGCGATGGACTGGAACACCAGGTCCAGTGGCACGCCGCGGtt                                    <  4:261764/149‑1 (MQ=255)
                                                                                                                 aTCAGGTTCTGCCCGAGGGTGCCACGTTTCAGCGACAGCCCTGCTTCGTAGCCTTCCTGCAACACGTTCAGGTTGATGCCGAAGCCGGCGTTGGCCGCCTCTGTGCCGGCGATGGACTGGAACACCAGGTCCAGTGGCACGCCGCGGtt                                    >  7:187794/1‑149 (MQ=255)
                                                                                                                  tCAGGTTCTGCCCGAGGGTGCCACGTTTCAGCGACAGCCCTGCTTCGTAGCCTTCCTGCAACACGTTCAGGTTGATGCCGAAGCCGGCGTTGgccgc                                                                                       >  6:55254/1‑97 (MQ=255)
                                                                                                                  tCAGGTTCTGCCCGAGGGTGCCACGTTTCAGCGACAGCCCTGCTTCGTAGCCTTCCTGCAACACGTTCAGGTTGATGCCGAAGCCGGCGTTGGCCGCCTCTGTGc                                                                               <  8:88993/105‑1 (MQ=255)
                                                                                                                  tCAGGTTCTGCCCGAGGGTGCCACGTTTCAGCGACAGCCCTGCTTCGTAGCCTTCCTGCAACACGTTCAGGTTGATGCCGAAGCCGGCGTTGGCCGCCTCTGTGc                                                                               >  7:88993/1‑105 (MQ=255)
                                                                                                                  tCAGGTTCTGCCCGAGGGTGCCACGTTTCAGCGACAGCCCTGCTTCGTAGCCTTCCTGCAACACGTTCAGGTTGATGCCGAAGCCGGCGTTGGCCGCCTCTGTGCCGGCGATGGACTGGAACACCAGGTCCAGTGGCACGCCGCGGtt                                    >  7:115777/1‑148 (MQ=255)
                                                                                                                  tCAGGTTCTGCCCGAGGGTGCCACGTTTCAGCGACAGCCCTGCTTCGTAGCCTTCCTGCAACACGTTCAGGTTGATGCCGAAGCCGGCGTTGGCCGCCTCTGTGCCGGCGATGGACTGGAACACCAGGTCCAGTGGCACGCCGCGGtt                                    >  7:281622/1‑148 (MQ=255)
                                                                                                                  tCAGGTTCTGCCCGAGGGAGCCACGTTTCAGCGACAGCCCTGCTTCGTAGCCTTCCTGCAACACGTTCAGGTTGATGCCGAAGCCGGCGTTGGCCGCCTCTGTGCCGGCGATGGACTGGAACACCAGGTCCAGTGGCACGCCGCGGTTg                                   >  5:278665/1‑149 (MQ=255)
                                                                                                                      gTTCTGCCCGAGGGTGCCACGTTTCAGCGACAGCCCTGCTTCGTAGCCTTCCTGCAACACGTTCAGGTTGATGCCGAAGCCGGCGTTGGCCGCCTCTGTGCCGGCGATGGAc                                                                    >  5:182125/1‑112 (MQ=255)
                                                                                                                      gTTCTGCCCGAGGGTGCCACGTTTCAGCGACAGCCCTGCTTCGTAGCCTTCCTGCAACACGTTCAGGTTGATGCCGAAGCCGGCGTTGGCCGCCTCTGTGCCGGCGATGGAc                                                                    <  6:182125/112‑1 (MQ=255)
                                                                                                                           gCCCGAGGGTGCCACGTTTCAGCGACAGCCCTGCTTCGTAGCCTTCCTGCAACACGTTCAGGTTGATGCCGAAGCCGGCGTTGGCCGCCTCTGTGCCGGCGATGGACTGGAACACCAGGTCCAGTGGCACGCCGCGGTTGATCGCTtc                           >  8:150244/1‑148 (MQ=255)
                                                                                                                            cccGAGGGTGCCACGTTTCAGCGACAGCCCTGCTTCGTAGCCTTCCTGCAACACGTTCAGGTTGATGCCGAAGCCGGCGTTGGCCGCCTCTGTGCCGGCGATGGACTGGAACACCAGGTCCAGTGGCACGCCGCGGTTGATCGCTtcga                         >  3:336140/1‑149 (MQ=255)
                                                                                                                             ccGAGGGTGCCACGTTTCAGCGACAGCCCTGCTTCGTAGCCTTCCTGCAACACGTTCAGGTTGATGCCGAAGCCGGCGTTGGCCGCCTCTGTGCCGGCGATGGACTGGAACAcc                                                           <  3:311845/114‑1 (MQ=255)
                                                                                                                             ccGAGGGTGCCACGTTTCAGCGACAGCCCTGCTTCGTAGCCTTCCTGCAACACGTTCAGGTTGATGCCGAAGCCGGCGTTGGCCGCCTCTGTGCCGGCGATGGACTGGAACAcc                                                           >  4:311845/1‑114 (MQ=255)
                                                                                                                              cGAGGGTGCCACGTTTCAGCGACAGCCCTGCTTCGTAGCCTTCCTGCAACACGTTCAGGTTGATGCCGAAGCCGGCGTTGGCCGCCTCTGTGCCGGCGATGGACTGGAACACCAGGTCCAGTGGCACGCCGCGGTTGATCGCTtcgatc                       <  7:150244/149‑1 (MQ=255)
                                                                                                                                 gttgtgcACTTTTCAGGGACACCCCTGCTTCGTAGCATTCGTGCAAGACGTTCAGGTTGTTGTCGAAGCCGGGGTTGGCCGACTCTGTGCCGGCGATGGACTGGAACACCAGGTCCAGTGGCACGCCGCGGTTGATCGCTTCGATCGAg                    <  6:364423/143‑1 (MQ=255)
                                                                                                                                 gggTGCCACGTTTCAGCGACAGCCCTGCTTCGTAGCCTTCCTGCAACACGTTCAGGTTGATGCCGAAGCCGGCGTTGGCCGCCTCTGTGCCGGCGATGGACTGGAACACCAGGTCCAGTGGCACGCCGCGGTTGATCGCTTCGATCGAg                    >  7:50621/1‑149 (MQ=255)
                                                                                                                                 gggTGCCACGTTTCAGCGACAGCCCTGCTTCGTAGCCTTCCTGCAACACGTTCAGGTTGATGCCGAAGCCGGCGTTGGCCGCCTCTGTGCCGGCGATGGACTGGAACACCAGGTCCAGTGGCACGCCGCGGTTGATCGCTTCGATCGAg                    <  6:150441/149‑1 (MQ=255)
                                                                                                                                 gggTGCCACGTTTCAGCGACAGCCCTGCTTCGTAGCCTTCCTGCAACACGTTCAGGTTGATGCCGAAGCCGGCGTTGGCCGCCTCTGTGCCGGCGATGGACTGGAACACCAGGTCCAGTGGCACGCCGCGGTTGATCGCTTCGATCGAg                    <  3:338380/149‑1 (MQ=255)
                                                                                                                                       cACGTTTCAGCGACAGCCCTGCTTCGTAGCCTTCCTGCAACACGTTCAGGTTTATGCCGAAGCCGGCGTTGGCCGCCTCTGTGCCGGCGATGGACTGGAACACCAGGTCCAGTGGCACGCCGCGGTTGATCGCTTCGATCGAGgtggtg              <  8:187794/149‑1 (MQ=255)
                                                                                                                                       cACGTTTCAGCGACAGCCCTGCTTCGTAGCCTTCCTGCAACACGTTCAGGTTGATGCCGAAGCCGGCGTTGGCCGCCTCTGTGCCGGCGATGGACTGGAACACCAGGTCCAGt                                                  <  4:277914/113‑1 (MQ=255)
                                                                                                                                       cACGTTTCAGCGACAGCCCTGCTTCGTAGCCTTCCTGCAACACGTTCAGGTTGATGCCGAAGCCGGCGTTGGCCGCCTCTGTGCCGGCGATGGACTGGAACACCAGGTCCAGt                                                  >  3:277914/1‑113 (MQ=255)
                                                                                                                                       cACGTTTCAGCGACAGCCCTGCTTCGTAGCCTTCCTGCAACACGTTCAGGTTGATGCCGAAGCCGGCGTTGGCCGCCTCTGTGCCGGCGATGGACTGGAACACCAGGTCCAGTGGCACGCCGCGGtt                                    >  3:219590/1‑127 (MQ=255)
                                                                                                                                       cACGTTTCAGCGACAGCCCTGCTTCGTAGCCTTCCTGCAACACGTTCAGGTTGATGCCGAAGCCGGCGTTGGCCGCCTCTGTGCCGGCGATGGACTGGAACACCAGGTCCAGTGGCACGCCGCGGtt                                    <  4:219590/127‑1 (MQ=255)
                                                                                                                                       cACGTTTCAGCGACAGCCCTGCTTCGTAGCCTTCCTGCAACACGTTCAGGTTGATGCCGAAGCCGGCGTTGGCCGCCTCTGTGCCGGCGATGGACTGGAACACCAGGTCCAGTGGCACGCCGCGGTTGATCGCTTCGATCGAggtggt               >  1:36251/1‑148 (MQ=255)
                                                                                                                                           tttCAGCGACAGCCCTGCTTCGTAGCCTTCCTGCAACACGTTCAGGTTGATGCCGAAGCCGGCGTTGGCCGCCTCTGTGCCGGCGATGGACTGGAACACCAGGTCCAGTGGCACGCCGCGGTTGATCGCTTCGATCGAGGTGGTGACGt          <  2:30275/149‑1 (MQ=255)
                                                                                                                                                gCGACAGCCCTGCTTCGTAGCCTTCCTGCAACACGTTCAGGTTGATGCCGAAGCCGGCGTTGGCCGCCTCTGTGCCGGCGATGGACTGGAACACCAGGTCCAGTGGCACGCCGCGGTTGATCGCTTCGATCGAGGTGGTGACGTGGGt      >  2:67220/1‑148 (MQ=255)
                                                                                                                                                  gACAGCCCTGCTTCGTAGCCTTCCTGCAACACGTTCAGGTTGATGCCGAAGCCGGCGTTGGCCGCCTCTGTGCCGGCGATGGACTGGAACACCAGGTCCAGTGGCACGCCGCGGTTGATCGCTTCGATCGAGGTGGTGACGTGGGTCAg   >  4:114632/1‑149 (MQ=255)
                                                                                                                                                   aCAGCCCTGCTTCGTAGCCTTCCTGCAACACGTTCAGGTTGATGCCGAAGCCGGCGTTGGCCGCCTCTGTGCCGGCGATGGACTGGAACACCAGGTCCAGTGGCACGCCGCGGTTGATCGCTTCGATCGAGGTGGTGACGTGGGTCAGc  <  8:281622/149‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                    
GGAACGGCTTGAAGTGACGAGCCACGGCATAGGCGCGGGTTTCGCAGGTCTGCTGGTCGACGCCGTGGTGGGCGTTGGCCGAGAGCGCGCTGCCCTGGCCGGTTTCGAAATACATCAGGTTCTGCCCGAGGGTGCCACGTTTCAGCGACAGCCCTGCTTCGTAGCCTTCCTGCAACACGTTCAGGTTGATGCCGAAGCCGGCGTTGGCCGCCTCTGTGCCGGCGATGGACTGGAACACCAGGTCCAGTGGCACGCCGCGGTTGATCGCTTCGATCGAGGTGGTGACGTGGGTCAGC  >  NC_002947/629228‑629523

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑