Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 648,287 A→C 15.4% intergenic (+56/+35) acoR → / ← accC acetoin catabolism regulatory protein/acetyl‑CoA carboxylase biotin carboxylase subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_002947648,2870AC15.4% 89.4 / 7.7 38intergenic (+56/+35)acoR/accCacetoin catabolism regulatory protein/acetyl‑CoA carboxylase biotin carboxylase subunit
Reads supporting (aligned to +/- strand):  ref base A (13/19);  new base C (4/2);  total (17/21)
Fisher's exact test for biased strand distribution p-value = 3.78e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CTTTGGGCATTTCGCGGGCGACGCTTTATCGGCGAGTGCATGAGCACCGCATCGACATGCCGCGGATGAAGGGGTAGAGCCCTGGGGCCGCGACGCGGCCCCAGATTCTGGCAGGCAATAAAAAACCCCGGCACAGGGCCGGGGTTCTGGTACATCACACT‑ACACGATCACTCCTGGTTGGCCAGTTTGTGCTCGAGGTAGTGAATGTTGACGCCGCCTTTGCAGAAGCCTTCATCACGCACCAGGTCGCGGTGCAGCGGGATGTTGGTCTTGATGCCGTC  >  NC_002947/648155‑648435
                                                                                                                                    |                                                                                                                                                     
cTTTGGGCATTTCGCTGCCGACGCTTTATCGGCGAGTGCATGAGCACCGCATCGACATGCCGCGGATGAAGGGGTAGAGCCCTGGGGCCGCGACGCGGCCCCAGATTCTGGCAGGCAATAAAAAACCCCGGCACAGGGCCGGGGTTCTg                                                                                                                                       <  1:120407/149‑1 (MQ=255)
   tGGGCATTTCGCGGGCGACGCTTTATCGGCGAGTGCATGAGCACCGCATCGACATGCCGCGGATGAAGGGGTAGCGCCCTGGGGCCGCGACGCGGCCCCAGATTCTGGCAGGCAATAAAGAACCCCGGCCCTGTGCCGGGGtttttt                                                                                                                                      <  3:137267/147‑5 (MQ=255)
    gggCATTTCGCGGGCGACGCTTTATCGGCGAGTGCATGAGCACCGCATCGACATGCCGCGGATGAAGGGGTAGAG‑CCTGGGGCCGCGACCCGGCCCCACAAAATGGCAGGCAATAAAAAAACCCCCCACAAGGCCGGGGTTCTGGttc                                                                                                                                   >  2:306926/1‑146 (MQ=255)
           tCGCGGGCGACGCTTTATCGGCGAGTGCATGAGCACCGCATCGACATGCCGCGGATGAAGGGGTAGAGCCCTGGGGCCGCGACGCGGCCCCAGATTCTGGCAGGCAATAAAAAACCCCGGCACAGGGCCGGGGTTCTGGTACATcaca                                                                                                                             <  3:41167/148‑1 (MQ=255)
                ggCGACGCTTTATCGGCGAGTGCATGAGCACCGCATCGACATGCCGCGGATGAAGGGGTAGAGCCCTGGGGCCGCGCCGCGGGCCCAGCTTCTGGCAGGGCATAAAAAAACCCCGCACAGGGGCCGGGTTGTGGTACAGAacacaaaac                                                                                                                       >  6:96124/1‑144 (MQ=255)
                   caCGTTTTCTCGGCGTGTGCATTAGCACCGCATCGACATGCCGCGGATCACTGGGTAGAGCCCTGGGGCCGCGACGCGGCCCCAGATTCTGGCAGGCAATAAAAAACCCCGGCACAGGGCCGGGGTTCTGGTACATCACACT‑ACACGAt                                                                                                                   <  5:45715/148‑1 (MQ=255)
                       cTTTATCGGCGAGTGCATGAGCACCGCATCGACATGCCGCGGATGAAGGGGTAGAGCCCTGGGGCCGCGACGCGGGCCCAGATTGGGGGAGGGAATAAAAAAACCCCGCCCAGGGCCGGGGGTCGGGTTCATAACACTAACACGaccct                                                                                                                >  6:169106/1‑145 (MQ=255)
                                      cATGAGCACCGCATCGACATGCCGCGGATGAAGGGGTAGAGCCCTGGGGCCGCGACGCGGCCCCAGATTCTGGCAGGCAATAAAAAACCCCGGCACAGGGCCGGGGTTCTGGTACATCACACT‑ACACGATCACTCCTGGTTGGCCAGtt                                                                                                >  5:353116/1‑149 (MQ=255)
                                       aTGAGCACCGCATCGACATGCCGCGGATGAAGGGGTAGAGCCCTGGGGCCGCGACGCGGCCCCAGATTCTGGCAGGCAATAAAAAACCCCGGCACAGGGCCGGGGTTCTGGTACATCACACT‑ACACGATCACTCCTGGTTGGCCAGttt                                                                                               <  6:353116/149‑1 (MQ=255)
                                               cGCATCGACATGCCGCGGATGAAGGGGTAGAGCCCTGGGGCCGCGACGCGGCCCCAGATTCTGGCAGGCAATAAAAAACCCCGGCACAGGGCCGGGGTTCTGGTACATCACACT‑ACACGATCACTCCTGGTTGGCCAGTTTGTGCTCGa                                                                                       <  5:96124/149‑1 (MQ=255)
                                                            cgcgGATGAAGGGGTAGAG‑CCTGGGGCCGCGACGCGGCCCCAGATTCTGGCAGGCAATAAAAAACCCCGGCACAGGGCCGGGGTTCTGGTACATCACACT‑ACACGATCACTCCTGGTTGGCCAGTTTGTGCTCGAGGTAGTGAATGTTg                                                                         <  1:306926/149‑1 (MQ=255)
                                                               ggATGAAGGGGTAGAGCCCTGGGGCCGCGACGCGGCCCCAGATTCTGGCAGGCAATAAAAAACCCCGGCACAGGGCCGGGGTTCTGGTACATCACACT‑ACACGATCACTCCTGGTTGGCCAGTTTGTGCTCGAGGTAGTGAATGTTGAc                                                                       <  8:238455/149‑1 (MQ=255)
                                                                gATGAAGGGGTAGAGCCCTGGGGCCGCGACCCGGCCCCCGAATATGGCAGGCAATAAAAAAAACCCGCCCAGGGGCCGGGGTGTGGTACAACACACA‑AAACCATCACTCCTGGGTGGCCAGTTTTTGGTCGAGGTAGTGTATGtttaag                                                                      >  2:68149/1‑145 (MQ=255)
                                                                     aGGGGTAGAGCCCTGGGGCCGCGACGCGGCCCCAGATTCTGGCAGGCAATAAAAAACCCCGGCACAGGGCCGGGGTTCTGGTACATCACACT‑ACACGATCACTCCTGGt                                                                                                         <  1:305444/109‑1 (MQ=255)
                                                                            gagCCCTGGGGCCGCGACGCGGCCCCAGATTCTGGCAGGCAATAAAAAACCCCGGCACAGGGCCGGGGTTCTGGTACATCACACT‑ACACGATCACTCCTGGTTGGCCAGTTtgt                                                                                             <  1:118129/114‑1 (MQ=255)
                                                                                tcTGGGGCCGCGTCGCGGCCCCAGATTCTGGCAGGCAATAAAAAACCCCGGCACAGGGCCGGGGTTCTGGTACATCACACT‑ACACGATCACTCCTGGTTGGCCAGTTTGTGCTCGAGGTa                                                                                   >  8:89789/2‑120 (MQ=255)
                                                                                     ggCCGCGACGCGGCCCCAGATTCTGGCAGGCAATAAAAAACCCCGGCACAGGGCCGGGGTTCTGGTACATCACACT‑ACACGATCACTCCTGGTTGGCCAGTTTGTGCTCGAGGTAGTGAATGTTGAc                                                                       >  2:161058/1‑127 (MQ=255)
                                                                                         gcgACGCGGCCCCAGATTCTGGCAGGCAATAAAAAACCCCGGCACAGGGCCGGGGTTCTGGTACATCACACT‑ACACGATCACTCCTGGTTGGCCAGTTTGTGCTCGAGGTAGTGAATGTTGACGCCGCCTTTGCAGAAGCCTtc                                                  >  5:189628/1‑144 (MQ=255)
                                                                                         gcgACGCGGCCCCAGATTCTGGCAGGCAATAAAAAACCCCGGCACAGGGCCGGGGTTCTGGTACATCACACT‑ACACGATCACTCCTGGTTGGCCAGTTTGTGCTCGAGGTAGTGAATGTTGACGCCGCCTTTGCAGAAGCCTtc                                                  <  6:189628/144‑1 (MQ=255)
                                                                                             cgcgGCCCCAGATGCTGGCAGGCTATAAAAAAGCCCGGCACAGGGCCGGGGTTCTGGTACATCACACT‑ACACGATCACTCCTGGTTGGCCAGTTTGTGCTCGAGGTAGTGAATGTTGACGCCGCCTTTGCAGAAGCCTTCATCACGCAc                                         <  4:223676/149‑1 (MQ=255)
                                                                                              gcgGCCCCAGATTCTTGCAGGCAATAAAAAACCCCGGCACAGGGCCGGGGTTCTGGTACATCACACT‑ACACGATCACTCCTGGTTGGCCAGTTTGTGCTCGAGGTAGTGAATGTTGACGCCGCCTTTGCAGAAGCCTtca                                                 <  2:215273/140‑1 (MQ=255)
                                                                                              gcgGCCCCAGATTCTGGCAGGCAATAAAAAACCCCGGCACAGGGCCGGGGTTCTGGTACATCACACT‑ACACGATCACTCCTGGTTGGCCAGTTTGTGCTCGAGGTAGTGa                                                                               <  6:308905/110‑1 (MQ=255)
                                                                                              gcgGCCCCAGATTCTGGCAGGCAATAAAAAACCCCGGCACAGGGCCGGGGTTCTGGTACATCACACT‑ACACGATCACTCCTGGTTGGCCAGTTTGTGCTCGAGGTAGTGa                                                                               >  5:308905/1‑110 (MQ=255)
                                                                                              gcgGCCCCAGATTCTGGCAGGCAATAAAAAACCCCGGCACAGGGCCGGGGTTCTGGTACATCACACT‑ACACGATCACTCCTGGTTGGCCAGTTTGTGCTCGAGGTAGTGAATGTTGACGCCGCCTTTGCAGAAGCCTtca                                                 >  1:215273/1‑140 (MQ=255)
                                                                                                ggCCCCAGATTCTGGCAGGCAATAAAAAACCCCGGCACAGGGCCGGGGTTCTGGTACATCACACT‑ACACGATCACTCCTGGTTGGCCAGTTTGTGCTCGAGGTAGTGAATGTTGACGCCGCCTTTGCAGAAGCCTTCATCACGc                                           <  7:11809/144‑1 (MQ=255)
                                                                                                ggCCCCAGATTCTGGCAGGCAATAAAAAACCCCGGCACAGGGCCGGGGTTCTGGTACATCACACT‑ACACGATCACTCCTGGTTGGCCAGTTTGTGCTCGAGGTAGTGAATGTTGACGCCGCCTTTGCAGAAGCCTTCATCACGc                                           >  8:11809/1‑144 (MQ=255)
                                                                                                    ccAGATTCTGGCAGGCAATAAAAAACCCCGGCACAGGGCCGGGGTTCTGGTACATCACACT‑ACACGATCACTCCTGGTTGGCCAGTTTGTGCTCGAGGTAGTGAATGTTGACGCCGCCTTTGCAGAAGCCTTCATCACGCACCAGGTcg                                  >  2:153402/1‑149 (MQ=255)
                                                                                                         ttCTGGCAGGCAATAAAAAACCCCGGCACAGGGCCGGGGTTCTGGTACATCACACT‑ACACGATCACTCCTGGTTGGCCAGTTTGTGCTCGAGGTAGTGAATGTTGACGCCGCCTTTGCAGAAGCCTTCATCACGCACCAGGTCGCGGTg                             >  5:266597/1‑149 (MQ=255)
                                                                                                            tGGCAGGCAATAAAAAACCCCGGCACAGGGCCGGGGTTCTGGTACATCACACT‑ACACGATCACTCCTGGTTGGCCAGTTTGTGCTCGAGGTAGTGAATGTTGACGCCGCCTTTGCAGAAGCCTTCATCACGCACCAGGTCGCGGTGCAg                          >  5:272488/1‑149 (MQ=255)
                                                                                                                    aaTAACAAACCCCGGCACAGGGCCGGGGTTCTGGTACATCACACT‑ACACGATCACTCCTGGTTGGCCAGTTTGTGCTCGAGGTAGTGAATGTTGACGCCGCCTTTGCAGAAGCCTTCATCACGCACCAGGTCGCGGTGCAGCGGGATGt                  <  7:186924/149‑1 (MQ=255)
                                                                                                                    aaTAAAAAACCCCGGCACAGGGCCGGGGTTCTGGTACATCACACT‑ACACGATCACTCCTGGTTGGCCAGTTTGTGCTCGAGGTAGTGAATGTTGACGCCGCCTTTGCAGAAGCCTTCATCACGCACCAGGTCGCGGTGCAGCGGGATGt                  >  6:326336/1‑149 (MQ=255)
                                                                                                                      tAAAAAACCCCGGCACAGGGCCGGGGTTCTGGTACATCACACT‑ACACGATCACTCCTGGTTGGCCAGTTTGTGCTCGAgg                                                                                     <  2:286091/80‑1 (MQ=255)
                                                                                                                         agaaCCCCGGCCCTGTGCCGGGGTTTTGGTACATCACACT‑ACACGATCACTCCTGGTTGGCCAGTTTGTGCTCGAGGTAGTGAATGTTGAcgccgc                                                                  <  2:86347/94‑1 (MQ=38)
                                                                                                                         agaaCCCCGGCCCTGTGCCGGGGTTTTGGTACATCACACT‑ACACGATCACTCCTGGTTGGCCAGTTTGTGCTCGAGGTAGTGAATGTTGAcgccgc                                                                  >  1:86347/3‑96 (MQ=38)
                                                                                                                         agaaCCCCGGCCCTGTGCCGGGGTTCTGGTACATCACACT‑ACACGATCACTCCTGGTTGGCCAGTTTGTGCTCGAGGTAGTGAATGTTGACGCCGCCTTTGCAGAAGCCTTCATCACGCACCAGGTCGCGGTGCAGCGGGATGTTGGTc             >  3:142209/3‑149 (MQ=255)
                                                                                                                             ccccGGCACAGGGCCGGGGTTCTGGTACATCACACT‑ACACGATCACTCCTGGTTGGCCAGTTTGTGCTCGAGGTAGTGAATGATGACGCCGCCTTTGCAGAAGCCTTCATCACGCACCAGGTCGCGGTGCAGCGGGATGTTGGTCTTGa         <  7:328701/149‑1 (MQ=255)
                                                                                                                              cccGGCACAGGGCCGGGGTTCTGGTACATCACACT‑ACACGATCACTCCTGGTTGGCCAGTTTGTGCTCGAGGTAGTGAATGTTGACGCCGCCTTTGCAGAAGCCTTCATCACGCACCAGGTCGCGGTGCAGCGGGATGTTGGTCTTGAt        <  6:272488/149‑1 (MQ=255)
                                                                                                                                 ggCACAGGGCCGGGGTTCTGGTACATCACACT‑ACACGATCACTCCTGGTTGGCCAGTTTGTGCTCGAGGTAGTGAATGTTGACGCCGCCTTTGCAGAAGCCTTCATCACGCACCAGGTCGCGGTGCAGCGGGATGTTGGGCTTGATGcc     <  6:266597/149‑1 (MQ=255)
                                                                                                                                    acaGGGCCGGGGTTCTGGTACATCACACT‑ACACGATCACTCCTGGTTGGCCAGTTTGTGCTCGAGGTAGTGAATGTTGACGCCGCCTTTGCAGAAGCCTTCATCACGCACCAGGTCGCGGTGCAGCGGGATGTTGGTCTTGATGCCGTc  <  1:68149/149‑1 (MQ=255)
                                                                                                                                    |                                                                                                                                                     
CTTTGGGCATTTCGCGGGCGACGCTTTATCGGCGAGTGCATGAGCACCGCATCGACATGCCGCGGATGAAGGGGTAGAGCCCTGGGGCCGCGACGCGGCCCCAGATTCTGGCAGGCAATAAAAAACCCCGGCACAGGGCCGGGGTTCTGGTACATCACACT‑ACACGATCACTCCTGGTTGGCCAGTTTGTGCTCGAGGTAGTGAATGTTGACGCCGCCTTTGCAGAAGCCTTCATCACGCACCAGGTCGCGGTGCAGCGGGATGTTGGTCTTGATGCCGTC  >  NC_002947/648155‑648435

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑