Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 675,591 A→T 13.8% A36A (GCT→GCA)  PP_0577 ← hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_002947675,5910AT13.8% 72.1 / 3.9 29A36A (GCT→GCA) PP_0577hypothetical protein
Reads supporting (aligned to +/- strand):  ref base A (12/13);  new base T (2/2);  total (14/15)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.38e-01

GCCGTTGCGCCAGCTCCAGCTGCGGTAGTGCCCATAATCACGAGCGGGCGCAGGGTAAGCACTGGCGTCGAAGGTGGTGGCCGCCTGGGCGGGGGCCGGTGGTAACGGGCGGCTGCTGGCCACATACGGGTTGCTGCCCTGGCAGCCAGCCAGGGCAATGGGCAGCAGGGCCAGGCACAACAGACGGTACGGCATGTATTTCTCCCGGCGCGGCGGATTAGCGGGGGCGGCAAACCCAGTGCAGGTAGCGGCCAAGCCCGGCGAAGGTGGGGTGGCGACGGTGGG  >  NC_002947/675444‑675728
                                                                                                                                                   |                                                                                                                                         
gCCGTTGCGCCAGCTCCAGCTGCGGTAGTGCCCATAATCACGAGCGGGCGCAGGGTAAGCACTGGCGTCGAAGGTGGTGGCCGCCTGGGCGGGGGCCGGTGGTAACGGGCGGCTGCTGGCCACATACGGGTTGCTGCCCTGGcagcca                                                                                                                                           <  7:128787/148‑1 (MQ=255)
 ccGTTGCGCCAGCTCCAGCTGCGGTAGTGCCCATAATCACGAGTGGGCGCAGGGTAAGCACTGGCGTCGAAGGTGGTGGCCGCCTGGGCGGGGGCCGGTGGTAACGGGCGGCTGCTGGCCACATACGGGTTGCTGCCCTGGcagccagc                                                                                                                                         <  8:270919/149‑1 (MQ=255)
   gTTGCGCCAGCTCCAGCTGCGGTAGTGCCCATAATCACGAGCGGGCGCAGGGTAAGCACTGGCGTCGAAGGTGGTGGCCGCCTGGGCGGGGGCCGGTGGTAACGGGCGGCTGCTGGCCACATACGGGTTGCTGCCCTGGCAgccagcca                                                                                                                                       <  4:18327/149‑1 (MQ=255)
   gTTGCGCCAGCTCCAGCTGCGGTAGTGCCAATAATCACGAGCGGGCGAAGGGTAAGCACTGGCGTCGAAGGTGGTGGCCGCCTGGGCGGGGGCCGGTGGTAACGGGCGGCTGCTGGCCACATACGGGTTGCTGCCCTGGcagccagc                                                                                                                                         >  3:143915/1‑147 (MQ=255)
         ccagctccagctGCGGTAGTGCCCATAATCACGAGCGAGCGCAGGGTAAGCACTGGCGTCGAAGGTGGTGGCCGCCTGGGCGGGGGCCGGTGGTAACGGGCGGCTGCTGGCCACATACGGGTTGCTGCCCTGGcagccagc                                                                                                                                         >  1:169403/1‑141 (MQ=255)
         ccagctccagctGCGGTAGTGCCCATAATCACGAGCGAGCGCAGGGTAAGCACTGGCGTCGAAGGTGGTGGCCGCCTGGGCGGGGGCCGGTGGTAACGGGCGGCTGCTGGCCACATACGGGTTGCTGCCCTGGcagccagc                                                                                                                                         <  2:169403/141‑1 (MQ=255)
                    tGCGGTAGTGCCCATAATCACGAGCGGGCGCAGGGTAAGCACTGGCGTCGAAGGTGGTGGCCGCCTGGGCGGGGGCCGGTGGTAACGGGCGGCTGCTGGCCACATACGGGTTGCTGCCCTGGCAGCCAGCCAggg                                                                                                                                    >  5:27876/1‑135 (MQ=255)
                    tGCGGTAGTGCCCATAATCACGAGCGGGCGCAGGGTAAGCACTGGCGTCGAAGGTGGTGGCCGCCTGGGCGGGGGCCGGTGGTAACGGGCGGCTGCTGGCCACATACGGGTTGCTGCCCTGGCAGCCAGCCAggg                                                                                                                                    <  6:27876/135‑1 (MQ=255)
                       ggTAGTGCCCATAATCACGAGCGGGCGCAGGGTAAGCACTGGCGTCGAAGGTGGTGGCCGCCTGGGCGGGGGCCGGTGGTAACGGGCGGCTGCTGGCCACATACGGGTTGCTGCCCTGGCAGCCAGCCAGGGCAATGGGCAGCGGGGc                                                                                                                    >  6:8067/1‑148 (MQ=255)
                               ccATAATCACGAGCGGGCGCAGGGTAAGCACTGGCGTCGAAGGTGGTGGCCGCCTGGGCGGGGGCCGGTGGTAACGGGCGGCTGCTGGCCACATACGGGTTGCTGCCCTGGCAGccagccag                                                                                                                                      <  3:31564/122‑1 (MQ=255)
                               ccATAATCACGAGCGGGCGCAGGGTAAGCACTGGCGTCGAAGGTGGTGGCCGCCTGGGCGGGGGCCGGTGGTAACGGGCGGCTGCTGGCCACATACGGGTTGCTGCCCTGGCAGccagccag                                                                                                                                      >  4:31564/1‑122 (MQ=255)
                                         gAGCGGGCGCAGGGTAAGCACTGGCGTCGAAGGTGGTGGCCGCCTGGGCGGGGGCCGGTGGTAACGGGCGGCTGCTGGCCACATACGGGTTGCTGCCCTGGCAGCCAGCCAGGGCAATggg                                                                                                                             >  2:316416/1‑121 (MQ=255)
                                         gAGCGGGCGCAGGGTAAGCACTGGCGTCGAAGGTGGTGGCCGCCTGGGCGGGGGCCGGTGGTAACGGGCGGCTGCTGGCCACATACGGGTTGCTGCCCTGGCAGCCAGCCAGGGCAATggg                                                                                                                             <  1:316416/121‑1 (MQ=255)
                                                 gcAGGGTAAGCACTGGCGTCGAAGGTGGTGGCCGCCTGGGCGGGGGCCGGTGGTAACGGGCGGCTGCTGGCCACATACGGGTTGCTGCCCTGGCAGCCAGCCAGGGCAATGGGCAGCAGGGCCAGGCACAACAGACGGTACGGCATGTa                                                                                         <  1:175065/149‑1 (MQ=255)
                                                  cAGGGTAAGCACTGGCGTCGAAGGTGGTGGCCGCCTGGGCGGGGGCCGGTGGTAACGGGCGGCTGCTGGCCACATACGGGTTGCTGCCCTGGCAGCCAGCCAGGGCAATGGGCAGCAGGGCCAGGCACAACAGACGGTACGGCATGTAt                                                                                        <  3:120338/149‑1 (MQ=255)
                                                  cAGGGTAAGCACTGGCGTCGAAGGTGGTGGCCGCCTGGGCGGGGGCCGGTGGTAACGGGCGGCTGCTGGCCACATACGGGTTGCTGCCCTGGCAGCCAGCCAGGGCAATGGGCAGCAGGGCCAGGCACAACAGACGGTACGGCATGTAt                                                                                        >  4:276068/1‑149 (MQ=255)
                                                      gTAAGCACTGGCGTCGAAGGTGGTGGCCGCCTGGGCGGGGGCCGGTGGTAACGGGCGGCTGCTGGCCACATACGGGTTGCTGCCCTGGCAGCCAGCCAGGGCAATGGGCAGCAGGGCCAGGCACAACAGACGGTACGGCATGTATTtct                                                                                    <  3:276068/149‑1 (MQ=255)
                                                                            gtggCCGCCTGGGCGGGGGCCGGTGGTAACGGGCGGCTGCTGGCCACATACGGGTTGCTGCCCTGGCAGCCAGCCAGGGCAATGGGCAGCGGGGCCAGGCACAACAGACGGTACGGCATGTATTTCTCCCGGCGCGGCGGATTAGCgg                                                               <  5:8067/148‑1 (MQ=255)
                                                                             tggCCGCCTGGGCGGGGGCCGGTGGTAACGGGCGGCTGCTGGCCACATACGGGTTGCTGCCCTGGCAGCCAGCCAGGGCAATGGGCAGCAGGGCCAGGCACAACAGACGGTACGGCATGTATTTCTCCCGGCGCGGCGGATTAGCgggg                                                             >  5:357693/1‑149 (MQ=255)
                                                                                    cTGGGCGGGGGCCGGTGGTAACGGGCGGCTGCTGGCCACATACGGGTTGCTGCCCTGGCAGCCAGCCAGGGCAATGGGCAGCAGGGCCAGGCACAACAGACGGTACGGCATGTATTTCTCCCGGCGCGGCGGATTAGCGGGGGCGGCa                                                       >  4:247491/1‑148 (MQ=255)
                                                                                      gggCGGGGGCCGGTGGTAACGGGCGGCTGCTGGCCACATACGGGTTGCTGCCCTGGCAGCCAGCCAGGGCAATGGGCAGCAGGGCCAGGCACAACAGACGGTACGGCATGTATTTCTCCCGGCGCGGTGGATTAGCGGGGGCGGCAAAc                                                    >  2:177980/1‑149 (MQ=255)
                                                                                         cGGGGGCCGGTGGTAACGGGCGGCTGCTGGCCACATACGGGTTGCTGCCCTGGCAGCCAGCCAGGGCAATGGGCAGCAGGGCCAGGCACAACAGACGGTACGGCATGTATTTCTCCCGGCGCGGCGGATTAGCGGGAGCGGCAAACCCa                                                 <  3:247491/149‑1 (MQ=255)
                                                                                             ggCCGGTGGTAACGGGCGGCTGCTGGCCACATACGGGTTGCTGCCCTGGCAGCCAGCCAGGGCAATGGGCAGCAGGGCCAGGCACAACAGACGGTACGGCATGTATTTCTCCCGGCGCGGCGGATTAGCGGGGGCGGCAAACCCAGTGc                                             <  6:357693/149‑1 (MQ=255)
                                                                                                     gtAACGGGCGGCTGCTGGCCACATACGGGTTGCTGCCCTGGCAGCCAGCCAGGGCAATGGGCAGCAGGGCCAGGCACAACAGACGGTACGGCAt                                                                                            >  7:139487/1‑94 (MQ=255)
                                                                                                     gtAACGGGCGGCTGCTGGCCACATACGGGTTGCTGCCCTGGCAGCCAGCCAGGGCAATGGGCAGCAGGGCCAGGCACAACAGACGGTACGGCAt                                                                                            <  8:139487/94‑1 (MQ=255)
                                                                                                               gctgctGGCCACATACGGGTTGCTGCCCTGGCAGCCAGCCAGGGCAATGGGCAGCAGGGCCAGGCACAACAGACGGTACGGCATGTATTTCTCCCGGCGCGGCGGATTAGCGGGggc                                                           <  6:145803/117‑1 (MQ=255)
                                                                                                                      gCCACATACGGGTTGCTGCCCTGGCAGCCAGCCAGGGCAATGGGCAGCAGGGCCAGGCACAACAGACGGTACGGCATGTATTTCTCCCGTCGCGGCGGATTATCGGGGGCGGCAAACCCAGTGCAGGTAGCGGCCAAGCCCGGCGAAgg                    >  3:292426/1‑149 (MQ=255)
                                                                                                                         acaTACGGGTTGCTGCCCTGGCAGCCAGCCAGGGCAATGGGCAGCAGGGCCAGGCACAACAGACGGTACGGCATGTATTTCTCCCGGCGCGGCGGa                                                                      >  4:183678/1‑96 (MQ=255)
                                                                                                                         acaTACGGGTTGCTGCCCTGGCAGCCAGCCAGGGCAATGGGCAGCAGGGCCAGGCACAACAGACGGTACGGCATGTATTTCTCCCGGCGCGGCGGa                                                                      <  3:183678/96‑1 (MQ=255)
                                                                                                                           aTACGGGTTGCTGCCCTGGCAGCCAGCCAGGGCAATGGGCAGCAGGGCCAGGCACAACAGACGGTACGGCATGTATTTCTCCCGGCGCGGCGGATTAGCGGGGGCGGCAAACCCAGTGCAGGTAGCGGCCAAGCCCGGCGAAggtggg                >  7:39130/1‑148 (MQ=255)
                                                                                                                           aTACGGGTTGCTGCCCTGGCAGCCAGCCAGGGCAATGGGCAGCAGGGCCAGGCACAACAGACGGTACGGCATGTATTTCTCCCGGCGCGGCGGATTAGCGGGGGCGGCAAACCCAGTGCAGGTAGCGGCCAAGCCCGGCGAAggtggg                <  8:39130/148‑1 (MQ=255)
                                                                                                                                gcccattgcCCTGGCTGGCTGCCAGGGCAATGGGCAGCAGGGCCAGGCACAACAGACGGTACGGCATGTATTTCTCCCGGCGCGGCGGATTAGCGGGGGCGGCAAACCCAGTGCAGGTAGCgg                                    <  1:124280/117‑1 (MQ=255)
                                                                                                                                gcccattgcCCTGGCTGGCTGCCAGGGCAATGGGCAGCAGGGCCAGGCACAACAGACGGTACGGCATGTATTTCTCCCGGCGCGGCGGATTAGCGGGGGCGGCAAACCCAGTGCAGGTAGCgg                                    >  2:124280/7‑123 (MQ=255)
                                                                                                                                   cattgcCCTGGCTGGCTGCCAGGGCAATGGGCAGCAGGGCCAGGCACAACAGACGGTACGGCATGTATTTCTCCCGGCGCGGCGGATTAGCGGGGGCGGCAAACCCAGTGCAg                                           >  2:311855/4‑113 (MQ=255)
                                                                                                                                   cattgcCCTGGCTGGCTGCCAGGGCAATGGGCAGCAGGGCCAGGCACAACAGACGGTACGGCATGTATTTCTCCCGGCGCGGCGGATTAGCGGGGGCGGCAAACCCAGTGCAg                                           <  1:311855/110‑1 (MQ=255)
                                                                                                                                       gcCCTGGCAGCCAGCCAGGGCAATGGGCAGCAGGGCCAGGCACAACAGACGGTACGGCATGTATTTCTCCCGGCGCGGCGGATTAGCGGGGGCGGCAAACCCAGTGCAGGTAGCGGCCAAGCCCGGCGAAGGTGGGGTGGCGACGGTgg   >  4:336354/1‑149 (MQ=255)
                                                                                                                                        cccTGGCAGCCAGCCATGGCAATGGGCAGCAGGGCCAGGCAAAACATACGGTACGGCATGTATTTCTGCCGGCGCGGCGGATTAGCGGGGGAGGCAAACCCAGTGCAGGTAGCGGCCAAGCCCGGCGAAGGTGGGGTGGCGAAGGTggg  <  4:292426/149‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                         
GCCGTTGCGCCAGCTCCAGCTGCGGTAGTGCCCATAATCACGAGCGGGCGCAGGGTAAGCACTGGCGTCGAAGGTGGTGGCCGCCTGGGCGGGGGCCGGTGGTAACGGGCGGCTGCTGGCCACATACGGGTTGCTGCCCTGGCAGCCAGCCAGGGCAATGGGCAGCAGGGCCAGGCACAACAGACGGTACGGCATGTATTTCTCCCGGCGCGGCGGATTAGCGGGGGCGGCAAACCCAGTGCAGGTAGCGGCCAAGCCCGGCGAAGGTGGGGTGGCGACGGTGGG  >  NC_002947/675444‑675728

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑