| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 702,766 | C→A | 16.2% | intergenic (+14/‑118) | PP_23SD → / → PP_5SD | 23S ribosomal RNA/5S ribosomal RNA |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 702,766 | 0 | C | A | 16.2% | 147.2 / 22.6 | 62 | intergenic (+14/‑118) | PP_23SD/PP_5SD | 23S ribosomal RNA/5S ribosomal RNA |
| Reads supporting (aligned to +/- strand): ref base C (26/26); new base A (3/7); total (29/33) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.12e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.51e-02 | |||||||||||
CTGGGATCTTGAATCCCCTAAAGGGCCGTCGAAGACTACGACGTTGATAGGTTGGGTGTGTAAGCGCTGTGAGGCGTTGAGCTAACCAATACTAATTGCCCGTGAGGCTTGACCATATAACACCCAAGCAATCTGCTACGCAGATTGCGGTGGTGAAGACGAAAGACCCGAAAATTCGTGCTTTACACAAGAGCTTCGCAAATATCGCATATCCGAATTCGCTGGGCTGTCCATCTGGACATTCTGGCAACAGAATTTCTTGACGACCATAGAGCATTGGAAC > NC_002947/702628‑702910 | cTGGGATCTTGAATCCCCTAAAGGGCCGTCGAAGACTACGACGTTGATAGGTTGGGTGTGTAAGCGCTGTGAGGCGTTGAGCTAACCAATACTAATTGCCCGTGAGGCTTGACCATATAACACCCAAGCAATCTGCTACGCAGATTGCg > 7:18401/1‑149 (MQ=31) ggATCTTGAATCCCCTAAAGGGCCGTCGAAGACTACGACGTTGATAGGTTGGGTGTGTAAGCGCTGTGAGGCGTTGAGCTAACGAATACTAATTGCCCGTGAGGCTTGACCATATAACACCCAAACAATCTGCGTAGCAGATTGcttg < 6:345604/148‑4 (MQ=11) ggATCTTGAATCCCCTAAAGGGCCGTCGAAGACTACGACGTTGATAGGTTGGGTGTGTAAGCGCTGTGAGGCGTTGAGCTAACCAATACTAATTGCCCGTGAGGCTTGACCATATAACACCCAAGCAATCTGCGTAGCAGATTGcttg < 1:80145/148‑4 (MQ=2) ggATCTTGAATCCCCTAAAGGGCCGTCGAAGACTACGACGTTGATAGGTTGGGTGTGTAAGCGCTGTGAGGCGTTGAGCTAACCAATACTAATTGCCCGTGAGGCTTGACCATATAACACCCAAGCAATCTGCGTAGCAGATTGctt < 6:211591/147‑3 (MQ=2) ggATCTTGAATCCCCTAAAGGGCCGTCGAAGACTACGACGTTGATAGGTTGGGTGTGTAAGCGCTGTGAGGCGTTGAGCTAACCAATACTAATTGCCCGTGAGGCTTGACCATATAACACCCAAGCAATCTGCGTAGCAGATTGctt < 7:275357/147‑3 (MQ=2) ggATCTTGAATCCCCTAAAGGGCCGTCGAAGACTACGACGTTGATAGGTTGGGTGTGTAAGCGCTGTGAGGCGTTGAGCTAACCAATACTAATTGCCCGTGAGGCTTGACCATATAACACCCAAGCAATCTGCGTAGCAGATTGctt < 3:295759/147‑3 (MQ=2) tCTTGAATCCCCTAAAGGGCCGTCGAAGACTACGACGTTGATAGGTTGGGTGTGTAAGCGCTGTGAGGCGTTGAGCTAACCAATACTAATTGCCCGTGAGGCTTGACCATATAACACCCAAGCAATCTGCTACGCAGATTGCggtggt > 7:129553/1‑148 (MQ=32) tCTTGAATCCCCTAAAGGGCCGTCGAAGACTACGACGTTGATAGGTTGGGTGTGTAAGCGCTGTGAGGCGTTGAGCTAACCAATACTAATTGCCCGTGAGGCTTGACCATATAACACCCAAGCAATCTGCTACGCAGATTGCggtggt < 8:129553/148‑1 (MQ=32) gAATCCCCTAAAGGGCCGTCGAAGACTACGACGTTGATAGGTTGGGTGTGTAAGCGCTGTGAGGCGTTGAGCTAACCAATACTAATTGCCCGTGAGGCTTGACCATATAACACCCAAGCAATCTGCTACGCAGATTGCGgtggtg < 6:186319/145‑1 (MQ=32) gAATCCCCTAAAGGGCCGTCGAAGACTACGACGTTGATAGGTTGGGTGTGTAAGCGCTGTGAGGCGTTGAGCTAACCAATACTAATTGCCCGTGAGGCTTGACCATATAACACCCAAGCAATCTGCTACGCAGATTGCGgtggtg < 3:308240/145‑1 (MQ=32) gAATCCCCTAAAGGGCCGTCGAAGACTACGACGTTGATAGGTTGGGTGTGTAAGCGCTGTGAGGCGTTGAGCTAACCAATACTAATTGCCCGTGAGGCTTGACCATATAACACCCAAGCAATCTGCGTAGCAGATTGcttgggt < 2:297425/144‑7 (MQ=11) gAATCCCCTAAAGGGCCGTCGAAGACTACGACGTTGATAGGTTGGGTGTGTAAGCGCTGTGAGGCGTTGAGCTAACCAATACTAATTGCCCGTGAGGCTTGACCATATAACACCCAAGCAATCTGCGTAGCAGATTGctt > 7:98877/1‑138 (MQ=2) gAATCCCCTAAAGGGCCGTCGAAGACTACGACGTTGATAGGTTGGGTGTGTAAGCGCTGTGAGGCGTTGAGCTAACCAATACTAATTGCCCGTGAGGCTTGACCATATAACACCCAAGCAATCTGCGTAGCAGATTGctt < 8:98877/140‑3 (MQ=2) aTCCCCTAAAGGGCCGTCGAAGACTACGACGTTGATAGGTTGGGTGTGTAAGCGCTGTGAGGCGTTGAGCTAACCAATACTAATTGCCCGTGAGGCTTGACCATATAACACCCAAGCAATCTGCTACGCAGATTGCGGTGGTGAAGAc < 8:18401/148‑1 (MQ=32) tCGAAGACTACGACGTTGATAGGTTGGGTGTGTAAGCGCTGTGAGGCGTTGAGCTAACCAATACTAATTGCCCGTGAGGCTTGACCATATAACACCCAAGCAATCTGCTACGCAGATTGCGGTGGTGAAGACGAAAGACCCGAAAATTc > 6:175658/1‑149 (MQ=32) tACGACGTTGATAGGTAGGGTGTGTAAGCGCTGTGAGGCGTTGAGCTAACCAATACTAATTGCCCGTGAGGCTTGACCATATAACACCCAAGCAATCTGCTACGCAGATTGCGGTGGTGAAGAAGAAAGACCCGAAAATTCGTGCttt > 2:40023/1‑148 (MQ=17) gacgTTGATAGGTTGGGTGTGTAAGCGCTGTGAGGCGTTGAGCTAACCAATACTAATTGCCCGTGAGGCTTGACCATATAACACCCAAGCAATCTGCTACGCAGATTGCGGTGGTGAAGACGAAAGACCCGAAAATTCGTGCTTTaca > 2:74615/1‑148 (MQ=32) gacgTTGATAGGTTGGGTGTGTAAGCGCTGTGAGGCGTTGAGCTAACCAATACTAATTGCCCGTGAGGCTTGACCATATAACACCCAAGCAATCTGCTACGCAGATTGCGGTGGTGAAGACGAAAGACCCGAAAATTCGTGCTTTaca > 6:321433/1‑148 (MQ=32) gTTGATAGGTTGGGTGTGTAAGCGCTGTGAGGCGTTGAGCTAACCAATACTAATTGCCCGTGAGGCTTGACCATATAACACCCAAGCAATCTGCTACGCAGATTGCGGTGGTGAAGACGAAAGACCCGAAAATTCGTGCTTTacac > 8:234916/1‑146 (MQ=32) tGATAGGTTGGGTGTGTAAGCGCTGTGAGGCGTTGAGCTTACCAATACTAATTGCGCGTGAGGCTTGACCATATAACACCCATGAAATCTGCTACTCAGATTGCGGTGGTGAAGACTAAAGACCCGAAAATTCGTGCTTTACACAaga < 1:40023/148‑1 (MQ=11) gATAGGTTGGGTGTGTAAGCGCTGTGAGGCGTTGAGCTAACCAATACTAATTGCCCGTGAGGCTTGACCATATAACACCCAAGCAATCTGCTACGCAGATTGCGGTGGTGAAGACGAAAGACCCGAAAATTCGTGCTTTacac > 1:30158/1‑143 (MQ=32) gATAGGTTGGGTGTGTAAGCGCTGTGAGGCGTTGAGCTAACCAATACTAATTGCCCGTGAGGCTTGACCATATAACACCCAAGCAATCTGCTACGCAGATTGCGGTGGTGAAGACGAAAGACCCGAAAATTCGTGCTTTacac < 2:30158/143‑1 (MQ=32) tttGGGTGTGTAAGCGCTGTGAGGCGTTGAGCTAACCAATACTAATTGCCCTTGAGGCTTGACCATATAACACCCAAGCAATCTGCTACGCAGATTGCGGTGGTGAAGACGaaa < 4:50615/113‑1 (MQ=17) gTTGGGTGTGTAAGCGCTGTGAGGCGTTGAGCTAACCAATACTAATTGCCCGTGAGGCTTGACCATATAACACACAAGCAATCTGCTACGCAGATTGCGGTGGTGAAGACGaaa > 3:50615/1‑114 (MQ=17) aGCGCTGTGAGGCGTTGAGCTAACCAATACTAATTGCCCGTGAGGCTTGACCATATAACACCCAAGCAATCTGCTACGCAGATTGCGGTGGTGAAGACGAAAGAc > 8:94468/1‑105 (MQ=32) aGCGCTGTGAGGCGTTGAGCTAACCAATACTAATTGCCCGTGAGGCTTGACCATATAACACCCAAGCAATCTGCTACGCAGATTGCGGTGGTGAAGACGAAAGAc < 7:94468/105‑1 (MQ=32) cTGTGAGGCGTTGAGCTAACCAATACTAATTGCCCGTGAGGCTTGACCATATAACACCCAAGCAATCTGCTACGCAGATTGc < 4:177398/82‑1 (MQ=31) cTGTGAGGCGTTGAGCTAACCAATACTAATTGCCCGTGAGGCTTGACCATATAACACCCAAGCAATCTGCTACGCAGATTGc > 3:177398/1‑82 (MQ=31) tgAGGCGTTGAGCTAACCAATACTAATTGCCCGTGAGGCTTGACCATATAACACCCAAGCAATCTGCTACGCAGATTGCGGTGGTGAAGACGAAAGAccc < 4:272936/100‑1 (MQ=33) gCGTTGAGCTAACCAATACTAATTGCCCGTGAGGCTTGACCATATAACACCCAAGCAATCTGCTACGCAGATTGCGGTGGTGAAGACGAAAGACCCGAAAATTCGTGCTTTACACAAGAGCTTCGCAAATATCGCATATCCGAATTCGc > 1:207041/1‑149 (MQ=32) gTTGAGCTAACCAATACTAATTGCCCGTGAGGCTTGACCATATAACACCCAAGCAATCTGCTACGCAGATTGCGGTGGTGAAGACGAAAGACCCGAAAATTCGTGCTTTACACAAGAGCTTCGCAAATATCGCATATCCGAATTCGCTg < 5:175658/149‑1 (MQ=32) cTAACCAATACTAATTGCCCGTGAGGCTTGACCATATAACACCCAAGCAATCTGCTACGCAGATTGCGGTGGTGAAGACGAAAGACCCGAAAATTCGTGCTTTa > 7:96779/1‑104 (MQ=32) cTAACCAATACTAATTGCCCGTGAGGCTTGACCATATAACACCCAAGCAATCTGCTACGCAGATTGCGGTGGTGAAGACGAAAGACCCGAAAATTCGTGCTTTa < 8:96779/104‑1 (MQ=32) cTAACCAATACTAATTGCCCGTGAGGCTTGACCATATAACACCCAAGCAATCTGCTACGCAGATTGCGGTGGTGAAGACGAAAGACCCGAAAATTCGTGCTTTACACAAGAGCTTCGCAAATATCGCATATCCGAATTCGCTGGGCTGt < 2:207041/149‑1 (MQ=32) cAATACTAATTGCCCGTGAGGCTTGACCATATAACACCCAAGCAATCTGCTACGCAGATTGCGGTGGTGAAGACGAAAGACCCGAAAATTCGTGCTTTACACAAGAGCTTCGCAAATATCGCATATCCGAATTCGCTGGGCTGTCCATc > 8:124459/1‑149 (MQ=32) cAATACTAATTGCCCGTGAGGCTTGACCATATAACACCCAAGCAATCTGCTACGCAGATTGCGGTGGTGAAGACGAAAGACCCGAAAATTCGTGCTTTACACAAGAGCTTCGCAAATATCGCATATCCGAATTCGCTGGGCTGTCCATc > 4:326660/1‑149 (MQ=32) tAATTGCCCGTGAGGCTTGACCATATAACACCCAAGCAATCTGCTACGCAGATTGCGGTGGTGAAGACGAAAGACCCGAAAATTCGTGCTTTACACAAGAGCTTCGCAAATATCGCATATCCGAATTCGCt < 4:215263/131‑1 (MQ=32) tAATTGCCCGTGAGGCTTGACCATATAACACCCAAGCAATCTGCTACGCAGATTGCGGTGGTGAAGACGAAAGACCCGAAAATTCGTGCTTTACACAAGAGCTTCGCAAATATCGCATATCCGAATTCGCt < 8:291711/131‑1 (MQ=32) tAATTGCCCGTGAGGCTTGACCATATAACACCCAAGCAATCTGCTACGCAGATTGCGGTGGTGAAGACGAAAGACCCGAAAATTCGTGCTTTACACAAGAGCTTCGCAAATATCGCATATCCGAATTCGCt > 7:291711/1‑131 (MQ=32) tAATTGCCCGTGAGGCTTGACCATATAACACCCAAGCAATCTGCTACGCAGATTGCGGTGGTGAAGACGAAAGACCCGAAAATTCGTGCTTTACACAAGAGCTTCGCAAATATCGCATATCCGAATTCGCt > 3:215263/1‑131 (MQ=32) tAATTGCCCGTGAGGCTTGACCATATAACACCCAAGCAATCTGCTACGCAGATTGCGGTGGTGAAGACGAAAGACCCGAAAATTCGTGCTTTACACAAGAGCTTCGCAAATATCGCATATCCGAATTCGCTGGGCTGTCCATCTGGACa > 2:188912/1‑149 (MQ=32) tAATTGCCCGTGAGGCTTGACCATATAACACCCAAGCAATCTGCTACGCAGATTGCGGTGGTGAAGACGAAAGACCCGAAAATTCGTGCTTTACACAAGAGCTTCGCAAATATCGCATATCCGAATTCGCTGGGCTGTCCATCTGGACa < 7:124459/149‑1 (MQ=32) ccGTGAGGCTTGACCATATAACACCCAAGCAATCTGCTACGCAGATTGCGGTGGTGAAGACGAAAGACCCGAAAATTCGTGCTTTACACAAGAGCTTCGCAAATATCGCATATCAGAATTCGCTGGGCTGTCCATCTGGACATTCTgg > 6:10772/1‑148 (MQ=17) aCCATATAACACCCAAGCAATCTGCTACGCAGATTGCGGTGGTGAAGACGAAAGACCCGAAAATTCGt < 1:252525/68‑1 (MQ=35) aCCATATAACACCCAAGCAATCTGCTACGCAGATTGCGGTGGTGAAGACGAAAGACCCGAAAATTCGt > 2:252525/1‑68 (MQ=35) ccATATAACACCCAAGCAATCTGCTACGCAGATTGCGGTGGTGAAGACGAAAGACCCGAAAATTCGTGCTTTACACAAGAGCTTCGCAAATATCGCATATCCGAATTCGCTGGGCTGTCCATCTGGACATTCTGGCAACAGAATTTCtt < 6:92037/149‑1 (MQ=32) atatAACACCCAAGCAATCTGCTACGCAGATTGCGGTGGTGAAGACGAAAGACCCGAAAATTCGTGCTTTACACAAGAGCTTCGCAAATATCGCATATCCGAATTCGCTGGGCTGTCCATCTGGACATTCTGGCAACAGAATTTCTTga < 1:74615/149‑1 (MQ=32) taACACCCAAGCAATCTGCTACGCAGATTGCGGTGGTGAAGACGAAAGACCCGAAAATTCGTGCTTTACACAAGAGCTTCGCAAATATCGCATATCCGAATTCGCTGGGCTGTCCATCTGGACAt < 6:231604/125‑1 (MQ=32) taACACCCAAGCAATCTGCTACGCAGATTGCGGTGGTGAAGACGAAAGACCCGAAAATTCGTGCTTTACACAAGAGCTTCGCAAATATCGCATATCCGAATTCGCTGGGCTGTCCATCTGGACAt < 8:115241/125‑1 (MQ=32) taACACCCAAGCAATCTGCTACGCAGATTGCGGTGGTGAAGACGAAAGACCCGAAAATTCGTGCTTTACACAAGAGCTTCGCAAATATCGCATATCCGAATTCGCTGGGCTGTCCATCTGGACAt > 5:231604/1‑125 (MQ=32) taACACCCAAGCAATCTGCTACGCAGATTGCGGTGGTGAAGACGAAAGACCCGAAAATTCGTGCTTTACACAAGAGCTTCGCAAATATCGCATATCCGAATTCGCTGGGCTGTCCATCTGGACAt > 7:115241/1‑125 (MQ=32) acCCAAGCAATCTGCTACGCAGATTGCGGTGGTGAAGACGAAAGACCCGAAAATTCGTGCTTTACACAAGAGCTTCGCAAATATCGCATATCCGAATTCGCt > 6:87344/1‑102 (MQ=32) acCCAAGCAATCTGCTACGCAGATTGCGGTGGTGAAGACGAAAGACCCGAAAATTCGTGCTTTACACAAGAGCTTCGCAAATATCGCATATCCGAATTCGCt < 5:87344/102‑1 (MQ=32) accgCAATCTGCGTAGCAGATTGCTGTGGTGAAGACGAAAGACCCGAAAATTCGTGCTTTACACAAGAGCTTCGCAAATATCGCATATCCGAATTCGCTGGGCTGTCCATCTGGACATTCTGGCAACAGAATTTCTTGACGACCATa > 5:354685/4‑147 (MQ=11) aaGCAATCTGCTACGCAGATTGCGGTGGTGAAGACGAAAGACCCGAAAATTCGTGCTTTACACAAGAGCTTCGCAAATATCGCATATCCGAATTCGCTGGGCTGTCCATCTGGACATTCTGGCAACAGAATTTCTTGACGACCATagag > 3:320097/1‑149 (MQ=32) cgCAATCTGCGTAGCAGATTGCGGTGGTGAAGACGAAAGACCCGAAAATTCGTGCTTTACACAAGAGCTTCGCAAATATCGCATATCCGAATTCGCTGGGCTGTCCATCTGGACATTCTGGCAACAGAATTTCTTGACGACCATagag > 8:3816/2‑148 (MQ=12) aaTCTGCTACGCAGATTGCGGTGGTGAAGACGAAAGACCCGAAAATTCGTGCTTTACACAAGAGCTTCGCAAATATCGCATATCCGAATTCGCTGGGCTGTCCATCTGGACATTCTGGCAACAGAATTTCTTGACGACCATAGAGCAtt < 5:10772/149‑1 (MQ=32) tGCTACGCAGATTGCGGTGGTGAAGACGAAAGACCCGAAAATTCGTGCTTTACACAAGAGCTTCGCAAATATCGCATATCCGAATTCGCTGGGCTGTCCATCTGGACATTCTGGCAACAGAATTTCTTGACGACCATAGAGCATTGGaa > 3:115139/1‑149 (MQ=32) cTACGCAGATTGCGGTGGTGAAGACGAAAGACCCGAAAATTCGTGCTTTACACAAGAGCTTCGCAAATCTCGCATATCCTAATTCGCTGGGCTGTCCATCTGGACATTCTGGCAACAGAATTTCTTGACGACCATAGAGCATTGGAac < 5:321433/148‑1 (MQ=14) cTACGCAGATTGCGGTGGTGAAGACGAAAGACCCGAAAATTCGTGCTTTACACAAGAGCTTCGCAAATATCGCATATCCGAATTCGCTGGGCTGTCCATCTGGACATTCTGGCAACAGAATTTCTTGACGACCATAGAGCATTgg < 7:234916/145‑1 (MQ=31) tACGCAGATTGCGGTGGTGAAGACGAAAGACCCGAAAATTCGTGCTTTACACAAGAGCTTCGCAAATATCGCATATCCGAATTCGCTGGGCTGTCCATCTGGACATTCTGGCAACAGAATTTCt < 6:186586/124‑1 (MQ=11) tACGCAGATTGCGGTGGTGAAGACGAAAGACCCGAAAATTCGTGCTTTACACAAGAGCTTCGCAAATATCGCATATCCGAATTCGCTGGGCTGTCCATCTGGACATTCTGGCAACAGAATTTCt > 5:186586/1‑124 (MQ=11) | CTGGGATCTTGAATCCCCTAAAGGGCCGTCGAAGACTACGACGTTGATAGGTTGGGTGTGTAAGCGCTGTGAGGCGTTGAGCTAACCAATACTAATTGCCCGTGAGGCTTGACCATATAACACCCAAGCAATCTGCTACGCAGATTGCGGTGGTGAAGACGAAAGACCCGAAAATTCGTGCTTTACACAAGAGCTTCGCAAATATCGCATATCCGAATTCGCTGGGCTGTCCATCTGGACATTCTGGCAACAGAATTTCTTGACGACCATAGAGCATTGGAAC > NC_002947/702628‑702910 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |