Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	716,286	C→T	6.8%	Q169* (CAG→TAG)	PP_0609 →	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	716,286	0	C	T	6.8%	171.6 / 2.2	59	Q169* (CAG→TAG)	PP_0609	hypothetical protein
Reads supporting (aligned to +/- strand):  ref base C (29/26);  new base T (2/2);  total (31/28)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.40e-01

CCTGACCGAAGTGTCTGTCGATGCAGCACGCAAGGCTGGGGACCTACTGGTGTACCCGGTCAGCAGGTATGTCTACCAGCTCCAGGACGATAAGCTGAGGTTCAAACGCGGAAACAGCAAATTTCAACCGTTGGTTGATAATGTGCAGGCGATGCGTGTGGAGCGTGTGCAGATGGTGGGGGGAGAACGGGTGGATATCAGCCTGACACTCTACGAACCCACGCTCAAGGTTCAGCAGCGCCATGAGTTGAGCGTCGCAATCCGCAACCCGGTCCCCGCTTCATGACT  >  NC_002947/716141‑716428                                                                                                                                                  |                                                                                                                                               ccTGACCGAAGTGTCTGTCGATGCAGCACGCAAGGCTGGGGACCTACTGGTGTACCCGGTCAGCAGGTATGTCTACCAGCTCCAGGACGATAAGCTGAGGTTCAAACGCGGAAACAGCAAATTTCAACCGTTGGTTGATAATGTGCAg                                                                                                                                              >  4:150358/1‑148 (MQ=255) ccTGACCGAAGTGTCTGTCGATGCAGCACGCAAGGCTGGGGACCTACTGGTGTACCCGGTCAGCAGGTATGTCTACCAGCTCCAGGACGATAAGCTGAGGTTCAAACGCGGAAACAGCAAATTTCAACCGTTGGTTGATAATGTGCAg                                                                                                                                              <  3:150358/148‑1 (MQ=255)  cTGACCGAAGTGTCTGTCGATGCAGCACGCAAGGCTGGGGACCTACTGGTGTACCCGGTCAGCAGGTATGTCTACCAGCTCCAGGACGATAAGCTGAGGTTCAAACGCGGAAACAGCAAATTTCAACCGTTGGTTGATAATGTGTAGGc                                                                                                                                            >  1:1527/1‑149 (MQ=255)   tGACCGAAGTGTCTGTCGATGCAGCACGCAAGGCTGGGGACCTACTGGTGTACCCGGTCAGCAGGTATGTCTACCAGCTCCAGGACGATAAGCTGAGGTTCAAACGCGGAAACAGCAAATTTCAACCGTTGGTTGATAATGTGCAGGc                                                                                                                                            >  8:225156/1‑148 (MQ=255)   tGACCGAAGTGTCTGTCGATGCAGCACGCAAGGCTGGGGACCTACTGGTGTACCCGGTCAGCAGGTATGTCTACCAGCTCCAGGACGATAAGCTGAGGTTCAAACGCGGAAACAGCAAATTTCAACCGTTGGTTGATAATGTGCAGGc                                                                                                                                            >  3:194400/1‑148 (MQ=255)       cGAAGTGTCTGTCGATGCAGCACGCAAGGCTGGGGACCTACTGGTGTACCCGGTCAGCAGGTATGTCTACCAGCTCCAGGACGATAAGCTGAGGTTCAAACGCGGAAACAGCAAATTTCAACCGTTGGTTGATAATGTGCAgg                                                                                                                                             <  4:1521/143‑1 (MQ=255)       cGAAGTGTCTGTCGATGCAGCACGCAAGGCTGGGGACCTACTGGTGTACCCGGTCAGCAGGTATGTCTACCAGCTCCAGGACGATAAGCTGAGGTTCAAACGCGGAAACAGCAAATTTCAACCGTTGGTTGATAATGTGCAgg                                                                                                                                             >  3:1521/1‑143 (MQ=255)         aaGTGTCTGTCGATGCAGCACGCAAGGCTGGGGACCTACTGGTGTACCCGGTCAGCAGGTATGTCTACCAGCTCCAGGACGATAAGCTGAGGTTCAAACGCGGAAACAGCAAATTTCAACCGTTGGTTGATAATGTGCAGGCGATGCgt                                                                                                                                     >  5:35584/1‑149 (MQ=255)              tctgtcGATGCAGCACGCAAGGCTGGGGACCTACTGGTGTACCCGGTCAGCAGCTATGTCTACCAGCTCCAGGACGATAAGCTGAGGTTCAAACGCGGAAACAGCAAATTTCAACCGTTGGTTGATAATGTGCAGGCGATGCGtgtg                                                                                                                                  >  3:165943/1‑147 (MQ=255)               ccgccGACGCAGCACCCCAGCCTGCGGACCTACTGGTGTACCCGGGCAGCAGGTATGTCTACCAGCTCCAGGACGATAAGCTGAGGTTCAAACGCGGAAACAGCAAATTTCAACCGATGGTTGATAATGTGCAGGCGATGCGTGTGGAg                                                                                                                               <  4:306100/145‑1 (MQ=255)                tgtcGATGCAGCACGCAAGGCTGGGGACCTACTGGTGTACCCGGTCAGCAGGTATGTCTACCAGCTCCAGGACGATAAGCTGAGGTTCAAACGCGGAAACAGCAAATTTCAACCGTTGGTTGATAATGTGCAGGCGATGCGTGTGGAGc                                                                                                                              >  6:269947/1‑149 (MQ=255)                  tcGATGCAGCACGCAAGGCTGGGGACCTACTGGTGTACCCGGTCAGCAGGTATGTCTACCAGCTCCAGGACGATAAGCTGAGGTTCAAACGCGGAAACAGCAAATTTCAACCGTTGGTTGATAATGTGCAGGCGATGCGTGTGGAGc                                                                                                                              <  7:225156/147‑1 (MQ=255)                  tcGATGCAGCACGCAAGGCTGGGGACCTACTGGTGTACCCGGTCAGCAGGTATGTCTACCAGCTCCAGGACGATAAGCTGAGGTTCAAACGCGGAAACAGCAAATTTCAACCGTTGGTTGATAATGTGCAGGCGATGCGTGTGGAGc                                                                                                                              <  5:269947/147‑1 (MQ=255)                    gATGCAGCACGCAAGGCTGGGGACCTACTGGTGTACCCGGTCAGCAGGTATGTCTACCAGCTCCAGGACGATAAGCTGAGGTTCAAACGCGGAAACAGCAAATTTCAACCGTTGGTTGATAATGTGCAGGCGATGCGtgtg                                                                                                                                  >  7:144250/1‑141 (MQ=255)                    gATGCAGCACGCAAGGCTGGGGACCTACTGGTGTACCCGGTCAGCAGGTATGTCTACCAGCTCCAGGACGATAAGCTGAGGTTCAAACGCGGAAACAGCAAATTTCAACCGTTGGTTGATAATGTGCAGGCGATGCGtgtg                                                                                                                                  <  6:305704/141‑1 (MQ=255)                    gATGCAGCACGCAAGGCTGGGGACCTACTGGTGTACCCGGTCAGCAGGTATGTCTACCAGCTCCAGGACGATAAGCTGAGGTTCAAACGCGGAAACAGCAAATTTCAACCGTTGGTTGATAATGTGCAGGCGATGCGtgtg                                                                                                                                  <  8:144250/141‑1 (MQ=255)                    gATGCAGCACGCAAGGCTGGGGACCTACTGGTGTACCCGGTCAGCAGGTATGTCTACCAGCTCCAGGACGATAAGCTGAGGTTCAAACGCGGAAACAGCAAATTTCAACCGTTGGTTGATAATGTGCAGGCGATGCGtgtg                                                                                                                                  >  5:305704/1‑141 (MQ=255)                     aTGCAGCACGCAAGGCTGGGGACCTACTGGTGTACCCGGTCAGCAGGTATGTCTACCAGCTCCAGGACGATAAGCTGAGGTTCAAACGCGGAAACAGCAAATTTCAACCGTTGGTTGATAATGTGCAGGCGATGCGTGTGGAGCGtgtg                                                                                                                         <  4:194400/149‑1 (MQ=255)                      tGCAGCACGCAAGGCTGGGGACCTACTGGTGTACCCGGTCAGCAGGTATGTCTACCAGCTCCAGGACGATAAGCTGAGGTTCAAACGCGGAAACAGCAAATTTCAACCGTTGGTTGATAATGTGCAGGCGATGCGTGTGGAGCGTGTGc                                                                                                                        <  8:194499/149‑1 (MQ=255)                         agcaCGCAAGGCTGGGGACCTACTGGTGTACCCGGTCAGCAGGTATGTCTACCAGCTCCAGGACGATAAGCTGAGGTTCAAACGCGGAAACAGCAAATTTCAACCGTTGGTTGATAATGTGCAGGCGATGCGTGTGGAGCGTGTGCAGa                                                                                                                     <  6:35584/149‑1 (MQ=255)                              ggaaGGCTGGGGAGCTACTGGTGTACCCGGTCAGCAGGTATGTCTACCAGCTCCAGGACGATAAGCTTAGGTTCAAACGCGGAAACAGCAAATTTCATCCGTTGGTTGATAATGTGCAGGCGATGCGTGTGGATCGTGTGTAGAtggtg                                                                                                                <  4:165943/147‑1 (MQ=255)                              gCAAGGCTGGGGACCTACTGGTGTACCCGGTCAGCAGGTATGTCTACCAGCTCCAGGACGATAAGCTGAGGTTCAAACGCGGAAACAGCAAATTTCAACCGTTGGTTGATAATGTGCAGGCGATGCGTGTGGAGCGTGTGCAGAtggtg                                                                                                                >  1:142195/1‑149 (MQ=255)                                aaGGCTGGGGACCTACTGGTGTACCCGGTCAGCAGGTATGTCTACCAGCTCCAGGACGATAAGCTGAGGTTCAAACGCGGAAACAGCAAATTTCAACCGTTGGTTGATAATGTGCAGGCGATGc                                                                                                                                       >  6:10670/1‑124 (MQ=255)                                aaGGCTGGGGACCTACTGGTGTACCCGGTCAGCAGGTATGTCTACCAGCTCCAGGACGATAAGCTGAGGTTCAAACGCGGAAACAGCAAATTTCAACCGTTGGTTGATAATGTGCAGGCGATGc                                                                                                                                       <  5:10670/124‑1 (MQ=255)                                  ggCTGGGGACCTACTGGTGTACCCGGTCAGCAGGTATGTCTACCAGCTCCAGGACGATAAGCTGAGGTTCAAACGCGGAAACAGCAAATTTCAACCGTTGGTTGATAATGTGCAGGCGATGCGTGTGGAGCGTGTGCAGATGGTggggg                                                                                                            <  8:281878/149‑1 (MQ=255)                                              aCTGGTGTACCCGGTCAGCAGGTATGTCTACCAGCTCCAGGACGATAAGCTGAGGTTCAAACGCGGAAACAGCAAATTTCAACCGTTGGTTGATAATGTGCAGGCGATGCGTGTGGAGCGTGTGCAGATGGTGGGGGGAGAACGGGTgg                                                                                                >  6:314027/1‑149 (MQ=255)                                                     tACCCGGTCAGCAGGTATGTCTACCAGCTCCAGGACGATAAGCTGAGGTTCAAACGCGGAAACAGCAAATTTCAACCGTTGGTTGATAATGTGCAGGCGATGCGTGTGGAGCGTGTGCAGATGGTGGGGGGAGAACGGGTGGATATCAg                                                                                         >  2:191147/1‑149 (MQ=255)                                                           gTCAGCAGGTATGTCTACCAGCTCCAGGACGATAAGCTGAGGTTCAAACGCGGAAACAGCAAATTTCAACCGTTGGTTGATAATGTGCAGGCGATGCGTGTGGAGCGTGTGCAGATGGTGGGGGGAGAACGGGTGGATATCAGCCTGa                                                                                    >  1:60524/1‑148 (MQ=255)                                                              agcagGTATGTCTACCAGCTCCAGGACGATAAGCTGAGGTTCAAACGCGGAAACAGCAAATTTCAACCGTTGGTTGATAATGTGCAGGCGATGCGTGTGGAGCGTGTGCAGATGGTGGGGGGAGAACGGGTGGATATCAGCCTGACAct                                                                                >  7:215043/1‑149 (MQ=255)                                                                cagGTATGTCTACCAGCTCCAGGACGATAAGCTGAGGTTCAAACGCGGAAACAGCAAATTTCAACCGTTGGTTGATAATGTGTAGGCGATGCGTGTGGAGCGTGTGCAGATGGTGGGGGGAGAACGGGTGGATATCAGCCTGACActct                                                                              <  2:1527/149‑1 (MQ=255)                                                                 agGTATGTCTACCAGCTCCAGGACGATAAGCTGAGGTTCAAACGCGGAAACAGCAAATTTCAACCGTTGGTTGATAATGTGCAGGCGATGCGTGTGGAGCGTGTGCAGATGGTGGGGGGAGAACGGGTGGATATCAGCCTGACACTCTa                                                                             <  2:142195/149‑1 (MQ=255)                                                                    tATGTCTACCAGCTCCAGGACGATAAGCTGAGGTTCAAACGCGGAAACAGCAAATTTCAACCGTTGGTTGATAATGTGCAGGCGATGCGTGTGGAGCGTGTGCAGATGGTGGGGGGAGAACGGGTgg                                                                                                <  4:137314/127‑1 (MQ=255)                                                                    tATGTCTACCAGCTCCAGGACGATAAGCTGAGGTTCAAACGCGGAAACAGCAAATTTCAACCGTTGGTTGATAATGTGCAGGCGATGCGTGTGGAGCGTGTGCAGATGGTGGGGGGAGAACGGGTgg                                                                                                <  4:32415/127‑1 (MQ=255)                                                                      tGTCTACCAGCTCCAGGACGATAAGCTGAGGTTCAAACGCGGAAACAGCAAATTTCAACCGTTGGTTGATAATGTGCAGGCGATGCGTGTGGAGCGTGTGCAGATGGTGGGGGGAGAACGGGTGGATATCAGCCTGACACTCTACGAAc                                                                        >  6:40230/1‑149 (MQ=255)                                                                       gTCTACCAGCTCCAGGACGATAAGCTGAGGTTCAAACGCGGAAACAGCAAATTTCAACCGTTGGTTGATAATGTGCAGGCGATGCGTGTGGAGCGTGTGCAGATGGTGGGGGGAGAACggg                                                                                                   >  5:190109/1‑121 (MQ=255)                                                                            ccAGCTCCAGGACGATAAGCTGAGGTTCAAACGCGGAAACAGCAAATTTCAACCGTTGGTTGATAATGTGCAGGCGATGCGTGTGGAGCGTGTGCAGAt                                                                                                                    >  5:39248/1‑99 (MQ=255)                                                                            ccAGCTCCAGGACGATAAGCTGAGGTTCAAACGCGGAAACAGCAAATTTCAACCGTTGGTTGATAATGTGCAGGCGATGCGTGTGGAGCGTGTGCAGAt                                                                                                                    <  6:39248/99‑1 (MQ=255)                                                                               gCTCCAGGACGATAAGCTGAGGTTCAAACGCGGAAACAGCAAATTTCAACCGTTGGTTGATAATGTGCAGGCGATGCGTGTGGAGCGTGTGCAGATGGTGGGGGGAGAACGGGTGGATATCAGCCTGACACTCTACGAACCCACGCTc                                                                >  5:339150/1‑148 (MQ=255)                                                                                    aGGACGATAAGCTGAGGTTCAAACGCGGAAACAGCAAATTTCAACCGTTGGTTGATAATGTGTAGGCGATGCGTGTGGAGCGTGTGCAGATGGTGGGGGGAGAACGGGTGGATATCAGCCTGACACTCTACGAACCCACGCTCAAGGtt                                                          >  3:294292/1‑149 (MQ=255)                                                                                     ggACGATAAGCTGAGGTTCAAACGCGGAAACAGCAAATTTCAACCGTTGGTTGATAATGTGCAGGCGATGCGTGTGGAGCGTGTGCAGATGGTGGGGGGAGAACGGGTGGATATCAGCCTGACACTCTACGAACCCACGCTGAAGGTTc                                                         <  2:60524/149‑1 (MQ=255)                                                                                      gACGATAAGCTGAGGTTCAAACGCGGAAACAGCAAATTTCAACCTTTGGTTGATAATGTGCAGGCGATGCGTGTGGAGCGTGTGCAGATGGTGGGGGGAGAACGGGTGGATATCAGCCTGACACTCTACGAACCCACGCTCAAGGTTca                                                        <  1:341490/149‑1 (MQ=255)                                                                                       aCGATAAGCTGAGGTTCAAACGCGGAAACAGCAAATTTCAACCGTTGGTTGATAATGTGCAGGCGATGCGTGTGGAGCGTGTGCAGATGGTGGGGGGAGAACGGGTGGATATCAGCCTGACACTCTACGAACCCACGCTCAAGGTTcag                                                       >  8:207196/1‑149 (MQ=255)                                                                                         gATAAGCTGAGGTTCAAACGCGGAAACAGCAAATTTCAACCGTTGGTTGATAATGTGCAGGCGATGCGTGTGGAGCGTGTGCAGATGGTGGGGGGAGAACGGGTGGATATc                                                                                           >  3:168003/1‑111 (MQ=255)                                                                                         gATAAGCTGAGGTTCAAACGCGGAAACAGCAAATTTCAACCGTTGGTTGATAATGTGCAGGCGATGCGTGTGGAGCGTGTGCAGATGGTGGGGGGAGAACGGGTGGATATc                                                                                           <  4:168003/111‑1 (MQ=255)                                                                                           tAAGCTGAGGTTCAAACGCGGAAACAGCAAATTTCAACCGTTGGTTGATAATGTGCAGGCGATGCGTGTGGAGCGTGTGCAGATGGTGGGGGGAGAACGGGTGGATATCAGCCTGACACTCTACGAACCCACGCTCAAGGTTcagcag                                                    <  6:339150/148‑1 (MQ=255)                                                                                           tAAGCTGAGGTTCAAACGCGGAAACAGCAAATTTCAACCGTTGGTTGATAATGTGCAGGCGATGCGTGTGGAGCGTGTGCAGATGGTGGGGGGAGAACGGGTGGATATCAGCCTGACACTCTACGAACCCACGCTCAAGGTTCagcagc                                                   >  7:198416/1‑149 (MQ=255)                                                                                                    gTTCAAACGCGGAAACAGCAAATTTCAACCGTTGGTTGATAATGTGCAGGCGATGCGTGTGGAGCGTGTGCAGATGGTGGGGGGAGAACGGGTGGATATCAGCCTGACACTCTACGAACCCACGCTCAAGGTTCAGCAGCGCCAt                                              >  6:278237/1‑145 (MQ=255)                                                                                                    gTTCAAACGCGGAAACAGCAAATTTCAACCGTTGGTTGATAATGTGCAGGCGATGCGTGTGGAGCGTGTGCAGATGGTGGGGGGAGAACGGGTGGATATCAGCCTGACACTCTACGAACCCACGCTCAAGGTTCAGCAGCGCCATGa                                            <  5:278237/147‑1 (MQ=255)                                                                                                        aaaCGCGGAAACAGCAAATTTCAACCGTTGGTTGATAATGTGCAGGCGATGCGTGTGGAGCGTGTGCAGATGGTGGGGGGAGAACGGGTGGATATCAGCCTGACACTCTACGAACCCACGCTCAAGGTTCAGCAGCGCCAt                                              <  3:70824/141‑1 (MQ=255)                                                                                                        aaaCGCGGAAACAGCAAATTTCAACCGTTGGTTGATAATGTGCAGGCGATGCGTGTGGAGCGTGTGCAGATGGTGGGGGGAGAACGGGTGGATATCAGCCTGACACTCTACGAACCCACGCTCAAGGTTCAGCAGCGCCAt                                              >  4:70824/1‑141 (MQ=255)                                                                                                                   cAGCAAATTTCAACCGTTGGTTGATAATGTGCAGGCGATGCGTGTGGAGCGTGTGCAGATGGTGGGGGGAGAACGGGTGGATATCAGCCTGACACTCTACGAACCCACGCTCAAGGTTCAGCAGCGCCATGAGTTGAGCGTCGCAATcc                           <  8:215043/149‑1 (MQ=255)                                                                                                                     gCAAATTTCAACCGTTGGTTGATAATGTGCAGGCGATGCGTGTGGAGCGTGTGCAGATGGTGGGGGGAGAACGGGTGGATATCAGCCTGACACTCTACGAACCCACGCTCAAGGTTCAGCAGCGCCATGAGTTGAGCGTCGCAATCCg                          >  4:266054/1‑148 (MQ=255)                                                                                                                       aaaTTTCAACCGTTGGTTGATAATGTGCAGGCGATGCGTGTGGAGCGTGTGCAGATGGTGGGGGGAGAACGGGTGGATATCAGCCTGACACTCTACGAACCCACGCTCAAgg                                                            >  6:205177/1‑112 (MQ=255)                                                                                                                                ccGTTGGTTGATAATGTGCAGGCGATGCGTGTGGAGCGTGTGCAGATGGTGGGGGGAGAACGGGTGGATATCAGCCTGACACTCTACGAACCCACGCTCAAGGTTCAGCAGCGCCATGAGTTGAGCGTCGCAATCCGCAACCCGGTccc              >  6:231871/1‑149 (MQ=255)                                                                                                                                   ttggttgATAATGTGTAGGCGATGCGTGTGGAGCGTGTGCAGATGGTGGGGGGAGAACGGGTGGATATCAGCCTGACACTCTACGAACCCACGCTCAAGGTTCAGCAGCGCCATGAGTTGAGCGTCGCAATCCGCAACCCGGTCCCCGc           <  4:294292/149‑1 (MQ=255)                                                                                                                                   ttggttgATAATGTGCAGGCGATGCGTGTGGAGCGTGTGCAGATGGTGGGGGGAGAACGGGTGGATATCAGCCTGACACTCTACGAACCCACGCTCAAGGTTCAGCAGCGCCATGAGTTGAGCGTCGCAATCCGCAACCCGGTCCCCGc           <  5:40230/149‑1 (MQ=255)                                                                                                                                      gttgATAATGTGCAGGCGATGCGTGTGGAGCGTGTGCAGATGGTGGGGGGAGAACGGGTGGATATCAGCCTGACACTCTACGAACCCACGCTCAAGGTTCAGCAGCGCCATGAGTTGAGCGTCGCAATCCGCAACCCGGTCCCCGCTTc        <  7:207196/149‑1 (MQ=255)                                                                                                                                           tAATGTGCAGGCGATGCGTGTGGAGCGTGTGCAGATGGTGGGGGGAGAACGGGTGGATATCAGCCTGACACTCTACGAACCCACGCTCAAGGTTCAGCAGCGCCATGAGTTGAGCGTCGCAATCCGCAACCCGGTCCCCGCTTCATGAc   >  5:142148/1‑149 (MQ=255)                                                                                                                                            aaTGTGCAGGCGATGCGTGTGGAGCGTGTGCAGATGGTGGGGGGAGAACGGGTGGATATCAGCCTGACACTCTACGAACCCACGCTCAAGGTTCAGCAGCGCCATGAGTTGAGCGTCGCAATCCGCAACCCGGTCCCCGCTTCATGACt  >  3:16530/1‑149 (MQ=255)                                                                                                                                                  |                                                                                                                                               CCTGACCGAAGTGTCTGTCGATGCAGCACGCAAGGCTGGGGACCTACTGGTGTACCCGGTCAGCAGGTATGTCTACCAGCTCCAGGACGATAAGCTGAGGTTCAAACGCGGAAACAGCAAATTTCAACCGTTGGTTGATAATGTGCAGGCGATGCGTGTGGAGCGTGTGCAGATGGTGGGGGGAGAACGGGTGGATATCAGCCTGACACTCTACGAACCCACGCTCAAGGTTCAGCAGCGCCATGAGTTGAGCGTCGCAATCCGCAACCCGGTCCCCGCTTCATGACT  >  NC_002947/716141‑716428

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑