Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 720,921 G→C 8.9% T92S (ACC→AGC) ‡ PP_0614 ← bifunctional N‑carbamoyl‑beta‑alanine amidohydrolase/allantoine amidohydrolase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_002947720,9210GC 8.9% 166.3 / 6.6 56T92S (ACC→AGC) ‡PP_0614bifunctional N‑carbamoyl‑beta‑alanine amidohydrolase/allantoine amidohydrolase
Reads supporting (aligned to +/- strand):  ref base G (27/24);  new base C (2/3);  total (29/27)
Fisher's exact test for biased strand distribution p-value = 6.64e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.51e-01

CTTCGTTGGTCCATACCACCACTTCCAGCGGGGCTTCGGTGGTCAGGTGCAGGTCGTTGAGCGTGCGCATCACTTCGAGGCCGGCCATGACGCCGTAGCAGCCATCGAACTTGCCGCCGGTGGGTTGGGTGTCGATGTGGCTGCCGGTCATGATGGGCGGCAGGGCAGGGTTGCGCCCTGGGCGGCGGGCGAAGATATTGCCGATGGCGTCGACGCTGACCGTGCAGCCGGCCTCCTCGCACCAATGGACGAACAGGTCGCGAGCCTGGCGGTCGAGGTCGGTCAGGGCCAGGCG  >  NC_002947/720775‑721069
                                                                                                                                                  |                                                                                                                                                    
cttcGTTGGTCCATACCACCACTTCCAGCGGGGCTTCGGTGGTCTGGTGCAGGTCGTTGAGCGTGCGCATCACTTCGAGGCCGGCCATGACGCCGTAGCAGCCATCGAACTTGCCGCCGGTGGGTTGGGTGTCGATGTGGCTGCCgg                                                                                                                                                      <  6:200636/147‑1 (MQ=255)
    gTTGGTCCATACCACCACTTCCAGCGGGGCTTCGGTGGTCAGGTGCAGGTCGTTGAGCGTGCGCATCACTTCGAGGCCGGCCATGACGCCGTAGCAGCCATCGAACTTGCCGCCGGTGGGTTGGGTGTCGATGTGGCTGCCGGTCatga                                                                                                                                                >  1:126082/1‑149 (MQ=255)
        gTCCATACCACCACTTCCAGCGGGGCTTCGGTGGTCAGGTGCAGGTCGTTGAGCGTGCGCATCACTTCGAGGCCGGCCATGACGCCGTAGCAGCCATCGAACTTGCCGCCGGTGGGTTGGGTGTCGATGTGGCTGCCGGTCATGATggg                                                                                                                                            <  7:89327/149‑1 (MQ=255)
               ccaccaCTTCCAGCGGGGCTTCGGTGGTCAGGTGCAGGTCGTTGAGCGTGCGCATCACTTCGAGGCCGGCCATGACGCCGTAGCAGCCATCGAACTTGCCGCCGGTGGGTTGGGTGTCGATGTGGCTGTCGCCCATCATGACCggcagc                                                                                                                                     <  2:269768/149‑2 (MQ=25)
               ccaccaCTTCCAGCGGGGCTTCGGTGGTCAGGTGCAGGTCGTTGAGCGTGCGCATCACTTCGAGGCCGGCCATGACGCCGTAGCAGCCATCGAACTTGCCGCCGGTGGGTTGGGTGTCGATGTGGCTGCCGCCCATCATGACCggcagc                                                                                                                                     <  2:261691/149‑2 (MQ=25)
                caccacTTCCAGCGGGGCTTCGGTGGTCAGGTGCAGGTCGTTGAGCGTGCGCATCACTTCGAGGCCGGCCATGACGCCGTAGCAGCCATCGAACTTGCCGCCGGTGGGTTGGGTGTCGATGTGGCTGCCGGTCATGATGGGCggcaggg                                                                                                                                    >  8:189838/1‑149 (MQ=255)
                            cGGGGCTTCGGTGGTCAGGTGCAGGTCGTTGAGCGTGCGCATCACTTCGAGGCCGGCCATGACGCCGTAGCAGCCATCGAACTTGCCGCCGGTGGGTTGGGTGTCGATGTGGCTGCCGGTCATGATGGGCGGCAGGGCAGGGTTGCGcc                                                                                                                        >  1:212837/1‑149 (MQ=255)
                                 cTTCGGTGGTCAGGTGCAGGTCGTTGAGCGTGCGCATCACTTCGAGGCCGGCCATGACGCCGTAGCAGCCATCGAACTTGCCGCCGGTGGGTTGGGTGTCGATGTGGCTGCCGCCCATCATGACCggcag                                                                                                                                      >  7:106228/1‑130 (MQ=25)
                                 cTTCGGTGGTCAGGTGCAGGTCGTTGAGCGTGCGCATCACTTCGAGGCCGGCCATGACGCCGTAGCAGCCATCGAACTTGCCGCCGGTGGGTTGGGTGTCGATGTGGCTGCCGCCCATCATGACCggcag                                                                                                                                      <  8:106228/130‑1 (MQ=25)
                                     ggtggtCAGGTGCAGGTCGTTGAGCGTGCGCATCACTTCGAGGCCGGCCATGACGCCGTAGCAGCCATCGAACTTGCCGCCGGTGGGTTGGGTGTCGATGTGGCTGCCGGTCATGATGGGCGGCAGGGCAGGGTTGCGCCCTGggcggc                                                                                                               >  8:26453/1‑149 (MQ=255)
                                         gtCAGGTGCAGGTCGTTGAGCGTGCGCATCACTTCGAGGCCGGCCATGACGCCGTAGCAGCCATCGAACTTGCCGCCGGTGGGTTGGGTGTCGATGTGGCTGCCGGTCATGATGGGCGGCAGGGCAGGGTTGCGCCCTGGGCggcggg                                                                                                            <  7:26453/148‑1 (MQ=255)
                                             ggTGCAGGTCGTTGAGCGTGCGCATCACTTCGAGGCCGGCCAGGACGCCGTAGCAGCCATCGAACTTGCCGCCGGTGGGGTGGGTGTCGATGTGGCTGCCGGTCATGATGGGCGGCAGGGCAGGGTTGCGCCCTGGGCGGCGGGCGAAg                                                                                                       <  8:20828/149‑1 (MQ=255)
                                              gTGCAGGTCGTTGAGCGTGCGCATCACTTCGAGGCCGGCCATGACGCCGTAGCAGCCATCGAACTTGCCGCCGGTGGGTTGGGTGTCGATGTGGCTGCCGGTCATGATGGGCGGCAGGGCAGGGTTGCGCCCTGGGCggcgggcg                                                                                                          >  2:250398/1‑145 (MQ=255)
                                              gTGCAGGTCGTTGAGCGTGCGCATCACTTCGAGGCCGGCCATGACGCCGTAGCAGCCATCGAACTTGCCGCCGGTGGGTTGGGTGTCGATGTGGCTGCCGGTCATGATGGGCGGCAGGGCAGGGTTGCGCCCTGGGCGGCGGGCGAAGa                                                                                                      <  7:189838/149‑1 (MQ=255)
                                                  aGGTCGTTGAGCGTGCGCATCACTTCGAGGCCGGCCATGACGCCGTAGCAGCCATCGAACTTGCCGCCGGTGGGTTGGGTGTCGATGTGGCTGCCGGTCATGATGGGCGGCAGGGCAGGGTTGCGCCCTGGGcggcgg                                                                                                             <  7:258827/138‑1 (MQ=255)
                                                  aGGTCGTTGAGCGTGCGCATCACTTCGAGGCCGGCCATGACGCCGTAGCAGCCATCGAACTTGCCGCCGGTGGGTTGGGTGTCGATGTGGCTGCCGGTCATGATGGGCGGCAGGGCAGGGTTGCGCCCTGGGcggcgg                                                                                                             >  8:258827/1‑138 (MQ=255)
                                                   ggTCGTTGAGCGTGCGCATCACTTCGAGGCCGGCCATGACGCCGTAGCAGCCATCGAACTTGCCGCCGGTGGGTTGGGTGTCGATGTGGCTGCCGGTCATGATGGGCGGCAGGGCAGGGTTGCGCCCTGGGCGGCGGGCGAAGATATTg                                                                                                 >  8:306095/1‑149 (MQ=255)
                                                    gTCGTTGAGCGTGCGCATCACTTCGAGGCCGGCCATGACGCCGTAGCAGCCATCGAACTTGCCGCCGGTGGGTTGGGTGTCGATGTGGCTGCCGGTCATGATGGGCGGCAGGGCAGGGTTGCGCCCTGGGCGGCGGGCGAAGATATTGc                                                                                                <  1:250398/149‑1 (MQ=255)
                                                       gTTGAGCGTGCGCATCACTTCGAGGCCGGCCATGACGCCGTAGCAGCCATCGAACTTGCCGCCGGTGGGTTGGGTGTCGATGTGGCTGCCGGTCATGATGGGCGGCAGGGCAGGGTTGCGCCCTGGGCGGCGGGCGAAGATATTGCCGa                                                                                             <  2:212837/149‑1 (MQ=255)
                                                          gAGCGTGCGCATCACTTCGAGGCCGGCCATGACGCCGTAGCAGCCATCGAACTTGCCGCCGGTGGGTTGGGTGTCGATGTGGCTGCCGGTCATGATGGGCGGCAGGGCAGGGTTGCGCCCTGGGCGGCGGGCGAAGATATTGCCGATg                                                                                           <  2:126082/148‑1 (MQ=255)
                                                                gcgcATCACTTCGAGGCCGGCCATGACGCCGTAGCAGCCATCGAACTTGCCGCCGGTGGGTTGGGTGTCGATGTGGCTGCCGGTCATGATGGGCGGCAGGGCAGGGTTGCGCCCTGGGCGGCGGGCGAAGATATTGCCGATGGCGTCGa                                                                                    >  7:63738/1‑149 (MQ=255)
                                                                   cATCACTTCGAGGCCGACCATGACGCCGTAGCAGCCATCGAACTTGCCGCCGGTGGGTTGGGTGTCGATGTGGCTGCCGGTCATGATGGGCGGCAGGGCAGGGTTGCGCCCTGGGCGGCGGGCGAAGATATTGCCGATGGCGTCGACGc                                                                                 >  3:123521/1‑149 (MQ=255)
                                                                   cATCACTTCGAGGCCGACCATGACGCCGTAGCAGCCATCGAACTTGCCGCCGGTGGGTTGGGTGTCGATGTGGCTGCCGGTCATGATGGGCGGCAGGGCAGGGTTGCGCCCTGGGCGGCGGGCGAAGATATTGCCGATGGCGTCGACGc                                                                                 <  4:123521/149‑1 (MQ=255)
                                                                        cTTCGAGGCCGGCCATGACGCCGTCGCAGCCATCGAACGTGCCGCCGGGGGGTAGGGTGTCGAGGTGGCTGCCGGGCCTGATGGGCGGCAGGGCAGGGTGGCGCCCTGGGCGGCGGGCGAAGAGATTGCCGCGGGCGTCGCCGCTGAcc                                                                            >  1:263005/1‑149 (MQ=255)
                                                                         ttCGAGGCCGGCCATGACGCCGTAGCAGCCATCGAACTTGCCGCCGGTGGGTTGGGTGTCGATGTGGCTGCCGGTCATGATGGGCGGCAGGGCAGGGTTGCGCCCTGGGCGGCGGGCGAAGATATTGCCGATGGCGTCGACGCTGAcag                                                                           >  1:322460/1‑147 (MQ=255)
                                                                             aGGCCGGCCATGACGCCGTAGCAGCCATCGAACTTGCCGCCGGTGGGTTGGGTGTCGATGTGGCTGCCGGTCATGATGGGCGGCAGGGCAGGGTTGCGCCCTGGGCGGCGGGCGAAGATATTGCCGATGGCGTCGACGCTGACCGTg                                                                         >  8:67726/1‑147 (MQ=255)
                                                                              ggccggccATGACGCCGTAGCAGCCATCGAACTTGCCGCCGGTGGGTTGGGTGTCGATGTGGCTGCCGGTCATGATGGGCGGCAGGGCAGGGTTGCGCCCTGGGCGGCGGGCGAAGATATTGCCGATGGCGTCGACGCTGACCGTGCAg                                                                      >  3:299576/1‑149 (MQ=255)
                                                                                 cggccATGACGCCGTAGCAGCCATCGAACTTGCCGCCGGTGGGTTGGGTGTCGATGTGGCTGCCGGTCATGATGGGCGGCAGGGCAGGGTTGCGCCCTGGGCGGCGGGCGAAGATATTGCCGATGGCGTCGACGCTGACCGTGCAGCCg                                                                   >  8:324686/1‑149 (MQ=255)
                                                                                 cggccATGACGCCGTAGCAGCCATCGAACTTGCCGCCGGTGGGTTGGGTGTCGATGTGGCTGCCGGTCATGATGGGCGGCAGGGCAGGGTTGCGCCCTGGGCGGCGGGCGAAGATATTGCCGATGGCGTCGACGCTGACCGTGCAGCCg                                                                   >  1:1549/1‑149 (MQ=255)
                                                                                        gACGCCGTAGCAGCCATCGAACTTGCCGCCGGTGGGTTGGGTGTCGATGTGGCTGCCGGTCATGATGGGCGGCAGGGCAGGGTTGCGCCCTGGGCGGCGGGCGAAGATATTGCCGATGGCGTCGACGCTGAGCGTGCAGCCGGcctcct                                                            >  2:312212/1‑149 (MQ=255)
                                                                                          cGCCGTAGCAGCCATCGAACTTGCCGCCGGTGGGTTGGGTGTCGATGTGGCTGCCGGTCATGATGGGCGGCAGGGCAGGGTTGCGCCCTGGGCGGCGGGCGAAGATATTGCCGATGGCGTCGACGCTGACCGTGCAGCCGGCCTCCTCg                                                          >  4:281699/1‑149 (MQ=255)
                                                                                           gCCGTAGCAGCCATCTAACTTGCCGCCGGTGGGTTGGGTGTCGTTGTGGCTGCCGGTCATGATGGGCGGCAGGGCAGGGTTGCGCCCTGGGCGGCGGGCGAAGATATTGCCGATGGCGTCGACGCTGACCGTGCAGCCGGcctcct                                                            <  6:294396/146‑1 (MQ=255)
                                                                                           gCCGTAGCAGCCATCGAACTTGCCGCCGGTGGGTTGGGTGTCGATGTGGCTGCCGGTCATGATGGGCGGCAGGGCAGGGTTGCGCCCTGGGCGGCGGGCGAAGATATTGCCGATGGCGTCGACGCTGACCGTGCAGCCGGCCTCCTCg                                                          >  5:294396/1‑148 (MQ=255)
                                                                                             cGTAGCAGCCATCGAACTTGCCGCCGGTGGGTTGGGTGTCGATGTGGCTGCCGGTCATGATGGGCGGCAGGGCAGGGTTGCGCCCTGGGCGGCGGGCGAAGATATTGCCGATGGCGTCGACGCTGACCGTGCAGCCGGCCTCCTCGCAc                                                       >  5:84997/1‑149 (MQ=255)
                                                                                             cGTAGCAGCCATCGAACTTGCCGCCGGTGGGTTGGGTGTCGATGTGGCTGCCGGTCATGATGGGCGGCAGGGCAGGGTTGCGCCCTGGGCGGCGGGCGAAGATATTGCCGATGGCGTCGACGCTGACCGTGCAGCCGGCCTCCTCGCAc                                                       <  7:306095/149‑1 (MQ=255)
                                                                                                 gcagcCATCGAACTTGCCGCCGGTGGGTTGGGTGTCGATGTGGCTGCCGGTCATGATGGGCGGCAGGGCAGGGTTGCGCCCTGGGCGGCGGGCGAAGATATTGCCGATGGCGTCGACGCTGACCGTGCAGCCGGCCTCCTCGCACCaa                                                    <  3:141737/148‑1 (MQ=255)
                                                                                                 gcagcCATCGAACTTGCCGCCGGTGGGTTGGGTGTCGATGTGGCTGCCGGTCATGATGGGCGGCAGGGCAGGGTTGCGCCCTGGGCGGCGGGCGAAGATATTGCCGATGGCGTCGACGCTGACCGTGCAGCCGGCCTCCTCGCACCAAt                                                   <  6:84997/149‑1 (MQ=255)
                                                                                                   agcCATCGAACTTGCCGCCGGTGGGTTGGGTGTCGATGTGGCTGCCGGTCATGATGGGCGGCAGGGCAGGGTTGCGCCCTGGGCGGCGGGCGAAGATATTGCCGATGGCGTCGACGCTGACCGTGCAGCCGGCCTCCTCGCACCAATgg                                                 <  4:299576/149‑1 (MQ=255)
                                                                                                    gcCATCGAACTTGCCGCCGGTGGGTTGGGTGTCGATGTGGCTGCCGGTCATGATGGGCGGCAGGGCAGGGTTGCGCCCTGGGCGGCGGGCGAAGATATTGCCGATGGCGTCGACGCTGACCGTGCAGCCGGCCTCCTCGCACCAATGGa                                                >  2:283692/1‑149 (MQ=255)
                                                                                                    gcCATCGAACTTGCCGCCGGGGGGTTGGGTGTCGATGTGGCTGCCGGTCATGATGGGCGGCAGGGCAGGGTTGCGCCCTGGGCGGCGGGCGAAGATATTGCCGATGGCGTCGACGCTGACCGTGCAGCCGGCCTCCTCGCACCAATGGa                                                <  7:150708/149‑1 (MQ=255)
                                                                                                     ccttCGAACTTGCCGCCGGTGGGTTGGGTTTCGATGTGGCTGCCGGTCATGATGGGCGGCAGGGCAGGGTTGCGCCCTGGGCGGCGGGCGAAGATATTGCTGATGGCGTCGACGCTGACCGTGCAGCTGGCCTCCTCGCAc                                                       <  8:52370/138‑1 (MQ=255)
                                                                                                     ccATCGAACTTGCCGCCGGTGGGTTGGGTGTCGATGTGGCTGCCGGTCATGATGGGCGGCAGGGCAGGGTTGCGCCCTGGGCGGCGGGCGAAGATATTGCCGATGGCGTCGACGCTGACCGTGCAGCCGGCCTCCTCGCACCAATGGAc                                               >  7:256867/1‑149 (MQ=255)
                                                                                                     ccATCGAACTTGCCGCCGGTGGGTTGGGTGTCGATGTGGCTGCCGGTCATGATGGGCGGCAGGGCAGGGTTGCGCCCTGGGCGGCGGGCGAAGATATTGCCGATGGCGTCGACGCTGACCGTGCAGCCGGCCTCCTCGCACCAATGGAc                                               <  7:324686/149‑1 (MQ=255)
                                                                                                     ccATCGAACTTGCCGCCGGTGGGTTGGGTGTAGATGTGGCTGCCGGTAATAATGGGCGACAGGACAGGGTTGCGCACTGGGCGGAGGGCGAAGATATTGACTATGGAGTCCACGCTGACAGTGCAGCCGGCCTCCTCGCACCatatcg                                                >  7:52370/1‑143 (MQ=21)
                                                                                                       aTCGAACTTGCCGCCGGTGGGTTGGGTGTCGATGTGGCTGCCGGTCATGATGGGCGGCAGGGCAGGGTTGCGCCCTGGGCGGCGGGCGAAGATATTGCCGATGGCGTCGACGCTGACCGTGCAGCCGGCCTCCTCGCACCAATGGACg                                              >  7:284480/1‑148 (MQ=255)
                                                                                                           aaCTTGCCGCCGGTGGGTTGGGTGTCGATGTGGCTGCCGGTCATGATGGGCGGCAGGGCAGGGTTGCGCCCTGGGCGGCGGGCGAAGATATTGCCGATGGCGTCGACGCTGACCGTg                                                                         <  2:173694/117‑1 (MQ=255)
                                                                                                           aaCTTGCCGCCGGTGGGTTGGGTGTCGATGTGGCTGCCGGTCATGATGGGCGGCAGGGCAGGGTTGCGCCCTGGGCGGCGGGCGAAGATATTGCCGATGGCGTCGACGCTGACCGTGCAGCCGGCCTCCTCGCACCAATGGACGAACAg                                         >  5:92372/1‑149 (MQ=255)
                                                                                                                       gTGGGTTGGGTGTCTATGTGGCTGCCGGTCATGATGGGCGGCAGGGCAGGGTTGCGCCCTGGGCGGCGGGCGAAGATATTGCCGATGGCGTCGACGCTGACCGTGCAGCCGGCCTCCTCGCACCAATGGACGAACAGGTCGCGAGCCTg                             <  8:284480/149‑1 (MQ=255)
                                                                                                                        ggggtggggtgTGGCTGTGGCTGCCGGGCAGGATGGGCGGCAGGGCAGGGGTGCGCCCTGGGCGGCGGGCGAAGATATGGCCGATGGCGTCGACGCTGCCCGTGCAGCCGGCCTCCTCGCACCAATGGACGAACAGGTCGCGAGCCTgg                            <  2:263005/148‑1 (MQ=255)
                                                                                                                         gggttgggtGTCGATGTGGCTGCCGGTCATGATGGGCGGCAGGGCAGGGTTGCGCCCTGGGCGGCGGGCGAAGATATTGCCGATGGCGTCGACGCTGACCGTGCAGCCGGCCTCCTCGCACCAATGGACGAACAGCTCGCGAGCCTGGc                           >  6:137170/1‑149 (MQ=255)
                                                                                                                                 tgtCGATGTGGCTGCCGGTCATGATGGGCGGCAGGGCAGGGTTGCGCCCTGGGCGGCGGGCGAAGATATTGCCGATGGCGTCGACGCTGACCGTGCAGCCGGCCTCCTCGCACCAATGGACGAACAGGTCGCGAGCCTGGCGGTCGAgg                   >  3:75999/1‑149 (MQ=255)
                                                                                                                                   tCGATGTGGCTGCCGGTCATGATGGGCGGCAGGGCAGGGTTGCGCCCTGGGCGGCGGGCGAAGATATTGCCGATGGCGTCGACGCTGACCGTGCAGCCGGCCTCCTCGCACCAATGGACGAACAGGTCGCGAGCCTGGCGGTCGAggtc                 <  7:67726/149‑1 (MQ=255)
                                                                                                                                         tgGCTGCCGGTCATGATGGGCGGCAGGGCAGGGTTGCGCCCTGGGCGGCGGGCGAAGATATTGCCGATGGCGTCGACGCTGACCGTGCAGCCGGCCTCGTCGCTCCAATGGACGAAGAGGTCGCGAGCCTGGCGTTCGAGGTCGGTCAg           <  5:137170/149‑1 (MQ=255)
                                                                                                                                         gcccTGCCGCCCATCATGACCGGCAGGGCAGGGTTGCGCCCTGGGCGGCGGGCGAAGATATTGCCGATGGCGTCGACGCTGACCGTGCAGCCGGCCTCCTCGCACCAATGGACGAACAGGTCGCGAGCCTGGCGGTCGAGGTCGGTCAg           >  3:32920/4‑149 (MQ=21)
                                                                                                                                           gCTGCCGGTCATGATGGGCGGCAGGGCAGGGTTGCGCCCTGGGCGGCGGGCGAAGATATTGCCGATGGCGTCGACGCTGACCGTGCAGCCGGCCTCCTCGCACCAATGGACGAACAGGTCGCGAGCCTGGCGGTCGAGGTCGGTCAggg         >  1:66943/1‑149 (MQ=255)
                                                                                                                                           gCTGCCGGTCATGATGGGCGGCAGGGCAGGGTTGCGCCCTGGGCGGCGGGCGAAGATATTGCCGATGGCGTCGACGCTGACCGTGCAGCCGGCCTCCTCGCACCAATGGACGAACAGGTCGCGAGCCTGGCGGTCGAGGTCGGTCAgg          <  3:281699/148‑1 (MQ=255)
                                                                                                                                               ccGGTCATGATGGGCGGCATGTCAGGGTTGCGCCCTGGGCGGCGGGCTAAGATATTGCCGATGGCGTCGACGCTGACCGTGCAGCCGGCCTCCTCGCACCAATGGACGAACAGGTCGCGAGCCTGGCGGTCGAGGTCGGTCAGGGCCAg     <  2:322460/149‑1 (MQ=255)
                                                                                                                                                  gTCATGATGGGCGGCAGGGCAGGGTTGCGCCCTGGGCGGCGGGCGAAGATATTGCCGATGGCGTCGACGCTGACCGTGCAGCCGGCCTCCTCGCACCAATGGACGAACAGGTCGCGAGCCTGGCGGTCGAGGTCGGTCAGGGCCAggcg  >  2:24231/1‑149 (MQ=255)
                                                                                                                                                  gTCATGATGGGCGGCAGGGCAGGGTTGCGCCCTGGGCGGCGGGCGAAGATATTGCCGATGGCGTCGACGCTGACCGTGCAGCCGGCCTCCTCGCACCAATGGACGAACAGGTCGCGAGCCTGGCGGTCGAGGTCGGTCAGGGCCAg     <  6:204102/146‑1 (MQ=255)
                                                                                                                                                  gTCATGATGGGCGGCAGGGCAGGGTTGCGCCCTGGGCGGCGGGCGAAGATATTGCCGATGGCGTCGACGCTGACCGTGCAGCCGGCCTCCTCGCACCAATGGACGAACAGGTCGCGAGCCTGGCGGTCGAGGTCGGTCAGGGCCAg     >  5:204102/1‑146 (MQ=255)
                                                                                                                                                  |                                                                                                                                                    
CTTCGTTGGTCCATACCACCACTTCCAGCGGGGCTTCGGTGGTCAGGTGCAGGTCGTTGAGCGTGCGCATCACTTCGAGGCCGGCCATGACGCCGTAGCAGCCATCGAACTTGCCGCCGGTGGGTTGGGTGTCGATGTGGCTGCCGGTCATGATGGGCGGCAGGGCAGGGTTGCGCCCTGGGCGGCGGGCGAAGATATTGCCGATGGCGTCGACGCTGACCGTGCAGCCGGCCTCCTCGCACCAATGGACGAACAGGTCGCGAGCCTGGCGGTCGAGGTCGGTCAGGGCCAGGCG  >  NC_002947/720775‑721069

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑