Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 723,207 T→C 7.4% I188V (ATC→GTC)  PP_0617 ← branched‑chain amino acid ABC transporter permease

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_002947723,2070TC 7.4% 150.9 / 3.1 54I188V (ATC→GTC) PP_0617branched‑chain amino acid ABC transporter permease
Reads supporting (aligned to +/- strand):  ref base T (24/26);  new base C (2/2);  total (26/28)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.87e-01

GTACAGCGCCCCGGCGATGCCGGTGATGGCGCCCGACAGCATGAACACCAGAATCTTGAAATGGCGGGTGTCGTAGCCGATGGCGGCGGCGCGGGTCT‑CGTTCTCACGGATAGCCAGCAGGGTGCTGCCGAATGGCGAACGGATCACCCGCAGGGCTGCGGCGAAGATCAACAGGAACAGCACCGCGACGAACACATAGAAGTGCCGTGGGTCGGCCAGGTCGACTAGCACATGGCCGGCGATTTCGATATTCGGCCGTGGCACATCAAGCAGGCCGTTGTCACCGCCG  >  NC_002947/723064‑723352
                                                                                                                                                |                                                                                                                                                 
gTACAGCGCCCCGGCGATGCCGGTGATGGCGCCCGACAGCATGAACACCAGAATCTTGAAATGGCGGGTGTCGTAGCCGATGGCGGCGGCGCGGGTCT‑CGTTCTCACGGATAGCCAGCAGGGTGCTGCCGAATGGCGAACGGATCAccc                                                                                                                                              >  5:95059/1‑149 (MQ=255)
atACAGCGCCCCGGCGATGCCGGTGATGGCGCCCGACAGCATGAACACCAGAATCTTGAAATGGCGGGTGTCGTAGCCGATGGCGGCGGCGCGGGTCT‑CGTTCTCACGGATAGCCAGCAGGGTGCTGCCGAATGGCGAACGGATCAccc                                                                                                                                              >  1:131000/2‑149 (MQ=255)
     gcgcCCCGGCGATGCCGGTGATGGCGCCCGACAGCATGAACACCAGAATCTTGAAATGGCGGGTGTCGTAGCCGATGGCGGCGGCGCGGGTCTCCGTTCTCACGGATAGCCAGCAGGGTGCTGCCGAATGGCGAACGGATCACCCGCAg                                                                                                                                          <  1:96344/149‑1 (MQ=255)
     gcgcCCCGGCGATGCCGGTGATGGCGCCCGACAGCATGAACACCAGAATCTTGAAATGGCGGGTGTCGTAGCCGATGGCGGCGGCGCGGGTCT‑CGTTCTCACGGATAGCCAGCAGGGTGCTGCCGAATGGCGAACGGATCACCCGCAgg                                                                                                                                         >  3:168682/1‑149 (MQ=255)
           cGGCGATGCCGGTGATGGCGCCCGACAGCATGAACACCAGAATCTTGAAATGGCGGGTGTCGTAGCCGATGGCGGCGGCGCGGGTCT‑CGTTCTCACGGATAGCCAGCAGGGTGCTGCCGAATGGCGAACGGATCACCCGCAGGGCTgcg                                                                                                                                   <  4:168682/149‑1 (MQ=255)
               gATGCCGGTGATGGCGCCCGACAGCATGAACACCAGAATCTTGAAATGGCGGGTGTCGTAGCCGATGGCGGCGGCGCGGGTCT‑CGTTCTCACGGATAGCCAGCAGGGTGCTGCCGAATGGCGAACGGATCACCCGCAGGGCTgcggcg                                                                                                                                >  6:285316/1‑148 (MQ=255)
               gATGCCGGTGATGGCGCCCGACAGCATGAACACCAGAATCTTGAAATGGCGGGTGTCGTAGCCGATGGCGGCGGCGCGGGTCT‑CGTTCTCACGGATAGCCAGCAGGGTGCTGCCGAATGGCGAACGGATCACCCGCAGGGCTgcggcg                                                                                                                                >  7:159159/1‑148 (MQ=255)
                aTGCCGGTGATGGCGCCCGACAGCATGAACACCAGAATCTTGAAATGGCGGGTGTCGTAGCCGATGGCGGCGGCGCGGGTCT‑CGTTCTCACGGATAGCCAGCAGGGTGCTGCCGAATGGCGAACGGATCACCCGCAGGGCTGCGGCGaa                                                                                                                              <  2:298492/149‑1 (MQ=255)
                aTGCCGGTGATGGCGCCCGACAGCATGAACACCAGAATCTTGAAATGGCGGGTGTCGTAGCCGATGGCGGCGGCGCGGGTCT‑CGTTCTCACGGATAGCCAGCAGGGTGCTGCCGAATGGCGAACGGATCACCCGCAGGGCTGCGGCGaa                                                                                                                              <  6:95059/149‑1 (MQ=255)
                aTGCCGGTGATGGCGCCCGACAGCATGAACACCAGAATCTTGAAATGGCGGGTGTCGTAGCCGATGGCGGCGGCGCGGGTCT‑CGTTCTCACGGATAGCCAGCAGGGTGCTGCCGAATGGCGAACGGATCACCCGCAGGGCTGCGGCGaa                                                                                                                              <  7:256415/149‑1 (MQ=255)
                aTGCCGGTGATGGCGCCCGACAGCATGAACACCAGAATCTTGAAATGGCGGGTGTCGTAGCCGATGGCGGCGGCGCGGGTCT‑CGTTCTCACGGATAGCCAGCAGGGTGCTGCCGAATGGCGAACGGATCACCCGCAGGGCTGCGGCGaa                                                                                                                              <  7:265883/149‑1 (MQ=255)
                    cGGTGATGGCGCCCGACAGCATGAACACCAGAATCTTGAAATGGCGGGTGTCGTAGCCGATGGCGGCGGCGCGGGTCT‑CGTTCTCACGGATAGCCAGCAGGGTGCTGCCGAATGGCGAACGGATCAcc                                                                                                                                               >  2:236402/1‑128 (MQ=255)
                    cGGTGATGGCGCCCGACAGCATGAACACCAGAATCTTGAAATGGCGGGTGTCGTAGCCGATGGCGGCGGCGCGGGTCT‑CGTTCTCACGGATAGCCAGCAGGGTGCTGCCGAATGGCGAACGGATCAcc                                                                                                                                               <  1:236402/128‑1 (MQ=255)
                      gTGATGGCGCCCGACAGCATGAACACCAGAATCTTGAAATGGCGGGTGTCGTAGCCGATGGCGGCGGCGCGGGTCT‑CGTTCTCACGGATAGCCAGCAGGGTGCTGCCGAATGGCGAACGGATCACCCGCAGGGCTGCGGCGAAGATCaa                                                                                                                        <  6:197738/149‑1 (MQ=255)
                       tGATGGCGCCCGACAGCATGAACACCAGAATCTTGAAATGGCGGGTGTCGTAGCCGATGGCGGCGGCGCGGGTCT‑CGTTCTCACGGATAGCCAGCAGGGTGCTGCCGAATGGCGAACGGATCACCCGCAGGGCTGCGGCGAAGATCAAc                                                                                                                       <  8:331842/149‑1 (MQ=255)
                        gATGGCGCCCGACAGCATGAACACCAGAATCTTGAAATGGCGGGTGTCGTAGCCGATGGCGGCGGCGCGGGTCT‑CGTTCTCACGGATAGCCAGCAGGGTGCTGCCGAATGGCGAACGGATCACCCGCAGGGCTGCGGCGAAGATCAACa                                                                                                                      <  2:131000/149‑1 (MQ=255)
                         aTGGCGCCTGACAGCATGAACACCAGAATCTTGAAATGGCGGGTGTCGTAGCCGATGGCGGCGGCGCGGGTCT‑CGTTCTCACGGATAGCCAGCAGGGTGCTGCCGAATGGCGAACGGATCACCCGCAGGGCTGCGGCGAAGATCAACAg                                                                                                                     <  3:320601/149‑1 (MQ=255)
                         aTGGCGCCCGACAGCATGAACACCAGAATCTTGAAATGGCGGGTGTCGTAGCCGATGGCGGCGGCGCGGGTCT‑CGTTCTCACGGATAGCCAGCAGGGTGCTGCCGAATGGCGAACGGATCACCCGCAGGGCTGCGGCGAAGATCAACa                                                                                                                      <  1:340333/148‑1 (MQ=255)
                                       cATGAACACCAGAATCTAGAAATGGCGGGTGTCGTAGCCGATGGCGGCGGCGCGGGTCT‑CGTTCTCACGGATAGCCAGCAGGGTGCTGCCGAATGGCGAACGGATCACCCGCAGGGCTGCGGCGAAGATCAACAGGAACAGCACCGcga                                                                                                       >  1:331068/1‑149 (MQ=255)
                                                cAGAATCTTGAAATGGCGGGTGTCGTAGCCGATGGCGGCGGCGCGGCTCT‑CGTTCTCACCGATAGCCAGCAGGGTGCTGACGAATGGCGAACCGATCAACCGCAGGGCTGCGGCGAAGAACAACAGGAACAGCACCGAGACGAAcaca                                                                                               >  1:329171/1‑148 (MQ=255)
                                                        tGAAATGGCGGGTGTCGTAGCCGATGGCGGCGGCGCGGGTCT‑CGTTCTCACGGATAGCCAGCAGGGTGCTGCCGAATGGCGAACGGATCACCCGCAGGGCTGCGGCGAAGATCAACAGGAACAGCACCGCGACGAACACATAGAAGTg                                                                                       <  2:331068/148‑1 (MQ=255)
                                                                 gggTGTCGTAGCCGATGGCGGCGGCGCGGGTCT‑CGTTCTCACGGATAGCCAGCAGGGTGCTGCCGAATGGCGAACGGATCACCCGCAGGGCTGCGGCGAAGATCAACAGGAACAGCACCGCGACGAACACATAGAAGTGCCGTgg                                                                                 <  3:232275/145‑1 (MQ=255)
                                                                 gggTGTCGTAGCCGATGGCGGCGGCGCGGGTCT‑CGTTCTCACGGATAGCCAGCAGGGTGCTGCCGAATGGCGAACGGATCACCCGCAGGGCTGCGGCGAAGATCAACAGGAACAGCACCGCGACGAACACATAGAAGTGCCGTgg                                                                                 >  4:232275/1‑145 (MQ=255)
                                                                 gggTGTCGTAGCCGATGGCGGCGGCGCGGGTCT‑CGTTCTCACGGATAGCCAGCAGGGTGCTGCCGAATGGCGAACGGATCACCCGCAGGGCTGCGGCGAAGATCAACAGGAACAGCACCGCGACGAACACATAGAAGTGCCGTGGGTCg                                                                             >  6:346642/1‑149 (MQ=255)
                                                                      tCGTAGCAGATGGCGGCGGCGCGGGTCT‑CGTTCTCACGGATAGCCATCAGGGTGCTGCCGAATGGCGAACTGATCACACGCAGGGCTGCTGCGAAGATCAACAGGAACAGCACCGCGACGAACACATAGAAGTGCCGTGGGTCGGCCAg                                                                        <  2:329171/149‑1 (MQ=255)
                                                                        gTAGCCGATGGCGGCGGCGCGGGTCT‑CGTTCTCACGGATAGCCAGCAGGGTGCTGCCGAATGGCGAACGGATCACCCGCAGGGCTGCGGCGAAGATCAACAGGAACAGCACCGCGACGAACACATAGAAGTGCCGTGGGTCGGCCAGGt                                                                      <  8:177044/149‑1 (MQ=255)
                                                                        gTAGCCGATGGCGGCGGCGCGGGTCT‑CGTTCTCACGGATAGCCAGCAGGGTGCTGCCGAATGGCGAACGGATCACCCGCAGGGCTGCGGCGAAGATCAACAGGAACAGCACCGCGACGAACACATAGAAGTGCCGTGGGTCGGCCAGGt                                                                      <  5:346642/149‑1 (MQ=255)
                                                                             cGATGGCGGCGGCGCGGGTCT‑CGTTCTCTCGGATAGCCAGCAGGGTGCTGCCGAATGGCGAACGGATCACCCGCAGGGCTGCGGCGAAGATCAACAGGAACAGCACCGCGACGAACACATAg                                                                                            >  1:196078/1‑122 (MQ=255)
                                                                             cGATGGCGGCGGCGCGGGTCT‑CGTTCTCACGGATAGCCAGCAGGGTGCTGCCGAATGGCGAACGGATCACCCGCAGGGCTGCGGCGAAGATCAACAGGAACAGCACCGCGACGAACACATAg                                                                                            <  2:196078/122‑1 (MQ=255)
                                                                              gATGGCGGCGGCGCGGGTCT‑CGTTCTCACGGATAGCCAGCAGGGTGCTGCCGAATGGCGAACGGATCACCCGCAGGGCTGCGGCGAAGATCAACAGGAACAGCACCGCGACGAACACATAGAAGTGCCGTGGGTCGGCCAGGTCGACTa                                                                <  8:159159/149‑1 (MQ=255)
                                                                                      cggcgCGGGTCT‑CGTTCTCACGGATAGCCAGCAGGGTGCTGCCGAATGGCGAACGGATCACCCGCAGGGCTGCGGCGAAGATCAACAGGAACAGCACCGCGACGAACACATAGAAGTGCCGTGGGTCGGCCAGGTCGACTAGCACATgg                                                        <  8:93308/149‑1 (MQ=255)
                                                                                             ggTCT‑CGTTCTCACGGATAGCCAGCAGGGTGCTGCCGAATGGCGAACGGATCACCCGCAGGGCTGCGGCGAAGATCAACAGGAACAGCACCGCGACGAACACATAGAAGTGCCGTGGGTCGGCCAGGTCGACTAGCACATGGCCGGCGa                                                 >  4:5019/1‑149 (MQ=255)
                                                                                              gTCT‑CGTTCTCACGGATAGCCAGCAGGGTGCTGCCGAATGGCGAACGGATCACCCGCAGGGCTGCGGCGAAGATCAACAGGAACAGCACCGCGACGAACACATAGAAGTGCCGTGGGTCGGCCAGGTCGACTAGCACATGGCCGGCGAt                                                <  5:285316/149‑1 (MQ=255)
                                                                                                   cGTTCTCACGGATAGCCAGCAGGGTGCTGCCGAATGGCGAACGGATCACCCGCAGGGCTGCGGCGAAGATCAACAGGAACAGCACCGCGACGAACACATAGAAGTGCCGTGGGTCGGCCAGGTCGACTAGCACATGGCCGGCGATTTc                                             >  7:313041/1‑148 (MQ=255)
                                                                                                          aCGGATAGCCAGCAGGGTGCTGCCGAATGGCGAACGGATCACCCGCAGGGCTGCGGCGAAGATCAACAGGAACAGCACCGCGACGAACACATAGAAGTGCCGTGGGTCGGCCAGGTCGACTAGCACATGGCCGGCGATTTCGATATTCg                                     >  5:131140/1‑149 (MQ=255)
                                                                                                               tAGCCAGCAGGGTGCTGCCGAATGGCGAACGGATCACCCGCAGGGCTGCGGCGAAGATCAACAGGAACAGCACCGCGACGAACACATAGAAGTGCCGTGGGTCGGCCAGGTCGACTAGCACATGGCCGGCGATTTCGATATTCGGCCGt                                <  3:5019/149‑1 (MQ=255)
                                                                                                                              tgcCGAATGGCGAACGGATCACCCGCAGGGCTGCGGCGAAGATCAACAGGAACAGCACCGCGACGAACACATAGAAGTGCCGTGGGTCGGCCAGGTCGACTAGCACATGGCCGGCGATTTCGATATTCGGCCGTGGCACATCAAGCAgg                 >  4:207338/1‑149 (MQ=255)
                                                                                                                              tgcCGAATGGCGAACGGATCACCCGCAGGGCTGCGGCGAAGATCAACAGGAACAGCACCGCGACGAACACATAGAAGTGCCGTGGGTCGGCCAGGTCGACTAGCACATGGCCGGCGATTTCGATATTCGGCCGTGGCACATCAAGCAgg                 >  2:121380/1‑149 (MQ=255)
                                                                                                                              tgcCGAATGGCGAACGGATCACCCGCAGGGCTGCGGCGAAGATCAACAGGAACAGCACCGCGACGAACACATAGAAGTGCCGTGGGTCGGCCAGGTCGACTAGCACATGGCCGGCGATTTCGATATTCGGCCGTGGCACATCAAGCAgg                 >  6:155392/1‑149 (MQ=255)
                                                                                                                                ccGAATGGCGAACGGATCACCCGCAGGGCTGCGGCGAAGATCAACAGGAACAGCACCGCGACGAACACATAGAAGTGCCGTGGGTCGGCCAGGTCGACTAGCACATGGCCGGCGATTTCGATATTCGGCCGTGGCACATCAAGCAGGcc               <  1:121380/149‑1 (MQ=255)
                                                                                                                                      ggCGAACGGATCACCCGCAGGGCTGCGGCGAAGATCAACAGGAACAGCACCGCGACGAACACATAGAAGTGCCGTGGGTCGGCCAGGTCGACTAGCACATGGCCGGCGATTTCGATATTCGGCCGTGGCACATCAAGCAGGCCGtt            <  3:265482/146‑1 (MQ=255)
                                                                                                                                      ggCGAACGGATCACCCGCAGGGCTGCGGCGAAGATCAACAGGAACAGCACCGCGACGAACACATAGAAGTGCCGTGGGTCGGCCAGGTCGACTAGCACATGGCCGGCGATTTCGATATTCGGCCGTGGCACATCAAGCAGGCCGtt            >  4:265482/1‑146 (MQ=255)
                                                                                                                                       gCGAACGGATCACCCGCAGGGCTGCGGCGAAGATCAACAGGAACAGCACCGCGACGAACACATAGAAGTGCCGTGGGTCGGCCAGGTCGACTAGCACATGGCCGGCGATTTCGATATTCGGCCGTGGCACATCAAGCAGGCCGTTGTCa        >  4:342560/1‑149 (MQ=255)
                                                                                                                                       gCGAACGGATCACCCGCAGGGCTGCGGCGAAGATCAACAGGAACAGCACCGCGACGAACACATAGAAGTGCCGTGGGTCGGCCAGGTCGACTAGCACATGGCCGGCGATTTCGATATTCGGCCGTGGCACATCAAGCAGGCCGTTGTCa        >  2:319079/1‑149 (MQ=255)
                                                                                                                                         gAACGGATCACCCGCAGGGCTGCGGCGAAGATCAACAGGAACAGCACCGCGACGAACACATAGAAGTGCCGTGGGTCGGCCAGGTCGACTAGCACATGGCCGGCGAttt                                              >  2:335140/1‑109 (MQ=255)
                                                                                                                                         gAACGGATCACCCGCAGGGCTGCGGCGAAGATCAACAGGAACAGCACCGCGACGAACACATAGAAGTGCCGTGGGTCGGCCAGGTCGACTAGCACATGGCCGGCGAttt                                              <  1:335140/109‑1 (MQ=255)
                                                                                                                                         gAACGGATCACCCGCAGGGCTGCGGCGAAGATCAACAGGAACAGCACCGCGACGAACACATAGAAGTGCCGTGGGTCGGCCAGGTCGACTAGCACATGGCCGGCGATTTCGATATTCGGcc                                  <  2:7636/121‑1 (MQ=255)
                                                                                                                                         gAACGGATCACCCGCAGGGCTGCGGCGAAGATCAACAGGAACAGCACCGCGACGAACACATAGAAGTGCCGTGGGTCGGCCAGGTCGACTAGCACATGGCCGGCGATTTCGATATTCGGcc                                  >  1:7636/1‑121 (MQ=255)
                                                                                                                                         gAACGGACCACCCGCAGGGCTGCGGCGAAGATCAACAGGAACAGCACCGCGACGAACACATAGAAGTGCCGTGGGTCGGCCAGGTCGACTa                                                                >  3:201843/1‑91 (MQ=255)
                                                                                                                                         gAACGGACCACCCGCAGGGCTGCGGCGAAGATCAACAGGAACAGCACCGCGACGAACACATAGAAGTGCCGTGGGTCGGCCAGGTCGACTa                                                                >  3:197297/1‑91 (MQ=255)
                                                                                                                                         gAACGGACCACCCGCAGGGCTGCGGCGAAGATCAACAGGAACAGCACCGCGACGAACACATAGAAGTGCCGTGGGTCGGCCAGGTCGACTa                                                                <  4:197297/91‑1 (MQ=255)
                                                                                                                                         gAACGGACCACCCGCAGGGCTGCGGCGAAGATCAACAGGAACAGCACCGCGACGAACACATAGAAGTGCCGTGGGTCGGCCAGGTCGACTa                                                                <  4:201843/91‑1 (MQ=255)
                                                                                                                                          aaCGGATCACCCGCAGGGCTGCGGCGAAGATCAACAGGAACAGCACCGCGACGAACACATAGAAGTGCCGTGGGTCGGCCAGGTCGACTAGCACATGGCCGGCGATTTCGATATTCGGCCGTGGCACATCAAGCAGGCCGTTGTCAccg     >  3:226725/1‑149 (MQ=255)
                                                                                                                                             ggATCACCCGCAGGGCTGCGGCGAAGATCAACAGGAACAGCACCGCGACGAACACATAGAAGTGCCGTGGGTCGGCCAGGTCGACTAGCACATGGCCGGCGATTTCGATATTCGGCCGTGGCACATCAAGCAGGCCGTTGTCAccgccg  >  1:268561/1‑149 (MQ=255)
                                                                                                                                                tCACCCGCAGGGCTGCGGCGAAGATCAACAGGAACAGCACCGCGACGAACACATAGAAGTGCCGTGGGTCGGCCAGGTCGACTAGCACATGGCCGGCGATTTCGATATTc                                      >  6:120583/1‑110 (MQ=255)
                                                                                                                                                tCACCCGCAGGGCTGCGGCGAAGATCAACAGGAACAGCACCGCGACGAACACATAGAAGTGCCGTGGGTCGGCCAGGTCGACTAGCACATGGCCGGCGATTTCGATATTc                                      <  5:120583/110‑1 (MQ=255)
                                                                                                                                                |                                                                                                                                                 
GTACAGCGCCCCGGCGATGCCGGTGATGGCGCCCGACAGCATGAACACCAGAATCTTGAAATGGCGGGTGTCGTAGCCGATGGCGGCGGCGCGGGTCT‑CGTTCTCACGGATAGCCAGCAGGGTGCTGCCGAATGGCGAACGGATCACCCGCAGGGCTGCGGCGAAGATCAACAGGAACAGCACCGCGACGAACACATAGAAGTGCCGTGGGTCGGCCAGGTCGACTAGCACATGGCCGGCGATTTCGATATTCGGCCGTGGCACATCAAGCAGGCCGTTGTCACCGCCG  >  NC_002947/723064‑723352

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑