Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	109,427	T→C	9.5%	F206F (TTT→TTC)	PP_0103 →	cytochrome c oxidase subunit 2

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	109,427	0	T	C	9.5%	166.7 / 7.0	63	F206F (TTT→TTC)	PP_0103	cytochrome c oxidase subunit 2
Reads supporting (aligned to +/- strand):  ref base T (34/23);  new base C (3/3);  total (37/26)
Fisher's exact test for biased strand distribution p-value = 6.84e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.48e-01

CAAAGGATGAGCACTACCTGCTGGAAGTCGACCAGCCGCTGGTGCTGCCGGTGGGCGCGAAAGTGCGCTTCCTGGTCACTGCTGCCGATGTGATCCACTCCTGGTGGGTGCCGGCCTTCGCGGTCAAAC‑GCGACGCCATCCCAGGCTTTGTCAACGAAGCCTGGA‑CCCGGATCGAAAAGCCAGGCATCTACCGCGGCCAGTGCACCGAGCTGTGCGGCAAGGACCACGGCTTCATGCCGGTGGTGGTCGAGGTCAAGTCCAAGGCGGATTACGACACCTGGCTTGGCGAGCGCAA  >  NC_002947/109279‑109573                                                                                                                                                      |                                                                                                                                                    cAAAGGATGAGCACTACCTGCTGGAAGTCGACCAGCCGCTGGTGCTGCCGGTGGGCGCGAAAGTGCGCTTCCTGGTCACTGCTGCCGATGTGATCCACTCCTGGTGGGTGCCGGCCTTCGCGGTCAAAC‑GCGACGCCATCCCAGGCttt                                                                                                                                                     <  4:135064/149‑1 (MQ=255)        tGAGCACTACCTGCTGGAAGTCGACCAGCCGCTGGTGCTGCCGGTGGGCGCGAAAGTGCGCTTCCTGGTCACTGCTGCCGATGTGATCCACTCCTGGTGGGTGCCGGCCTTCGCGGTCAAAC‑GCGACGCCATCCCAGGCTTTGTCAACg                                                                                                                                              <  4:27891/149‑1 (MQ=255)                 ccagcgggAAGTCGACCACCCGCTGGTGCGGCCGGTGGGCGCGAAAGTGCGCTGCCTGGTCACAGCTGCCGAGGTGAGCCACTCCTGGTGGGTGCCGGCCTTCGCGGTCACAC‑GCGACGCCATCCCAGGCTTCGTTGACAAAGCCTgg                                                                                                                                      <  6:355974/142‑1 (MQ=255)                     ctgGAAGTCGACCAGCCGCTGGTGCTGCCGGTGGGCGCGAAAGTGCGCTTCCTGGTCACTGCTGCCGATGTGATCCACTCCTGGTGGGTGCCGGCCTTCGCGGTCAAAC‑GCGACGCCATCCCAGGCTTTGTCAACGAAGCCTGGA‑CCCg                                                                                                                                >  1:66616/1‑149 (MQ=255)                        gAAGTCGACCAGCCGCTGGTGCTGCCGGTGGGCGCGAAAGTGCGCTTCCTGGTCACTGCTGCCGATGTGATCCACTCCTGGTGGGTGCCGGCCTTCGCGGTCAAAC‑GCGACGCCATCCCAGGCTTTGTCAACGAAGCCTGGA‑CCCGGAt                                                                                                                             >  2:72631/1‑149 (MQ=255)                           gTCGACCAGCCGCTGGTGCTGCCGGTGGGCGCGAAAGTGCGCTTCCTGGTCACTGCTGCCGATGTGATCCACTCCTGGTGGGTGCCGGCCTTCGCGGTCAAAC‑GCGACGCCATCCCAGGCTTTGTCAACGAAGCCTGGA‑CCCGGATCg                                                                                                                           >  8:170591/1‑148 (MQ=255)                              gACCAGCCGCTGGTGCTGCCGGTGGGCGCGAAAGTGCGCTTCCTGGTCACTGCTGCCGATGTGATCCACTCCTGGTGGGTGCCGGCCTTCGCGGTCAAAC‑GCGACGCCATCCCAGGCTTTGTCAACGAAGCCTGGA‑CCCGGATCGaaaa                                                                                                                       <  7:170591/149‑1 (MQ=255)                              gACCAGCCGCTGGTGCTGCCGGTGGGCGCGAAAGTGCGCTTCCTGGTCACTGCTGCCGATGTGATCCACTCCTGGTGGGTGCCGGCCTTCGCGGTCAAAC‑GCGACGCCATCCCAGGCTTCGTTGACAAAGCCTg                                                                                                                                       >  3:191419/1‑134 (MQ=255)                              gACCAGCCGCTGGTGCTGCCGGTGGGCGCGAAAGTGCGCTTCCTGGTCACTGCTGCCGATGTGATCCACTCCTGGTGGGTGCCGGCCTTCGCGGTCAAAC‑GCGACGCCATCCCAGGCTTCGTTGACAAAGCCTg                                                                                                                                       <  4:191419/134‑1 (MQ=255)                                ccAGCCGCTGGTGCTGCCGGTGGGCGCGAAAGTGCGCTTCCTGGTCACTGCTGCCGATGTGATCCACTCCTGGTGGGTGCCGGCCTTCGCGGTCAAAC‑GCGACGCCATCCCAGGCTTTGTCAACGAAGCCTGGA‑CCCGGATCGaaaa                                                                                                                       <  8:223761/147‑1 (MQ=255)                                ccAGCCGCTGGTGCTGCCGGTGGGCGCGAAAGTGCGCTTCCTGGTCACTGCTGCCGATGTGATCCACTCCTGGTGGGTGCCGGCCTTCGCGGTCAAAC‑GCGACGCCATCCCAGGCTTTGTCAACGAAGCCTGGA‑CCCGGATCGAAAAGc                                                                                                                     <  2:35251/149‑1 (MQ=255)                                  aGCCGCTGGTGCTGCCGGTGGGCGCGAAAGTGCGCTTCCTGGTCACTGCTGCCGATGTGATCCACTCCTGGTGGGTGCCGGCCTTCGCGGTCAAAC‑GCGACGCCATCCCAGGCTTTGTCAACGAAGCCTGGA‑CCCGGATCGAAAAGc                                                                                                                     >  4:315987/1‑147 (MQ=255)                                   gCCGCTGGTGCTGCCGGTGGGCGCGAAAGTGCGCTTCCTGGTCACTGCTGCCGATGTGATCCACTCCTGGTGGGTGCCGGCCTTCGCGGTCAAAC‑GCGACGCCATCCCAGGCTTTGTCAACGAAGCCTGGA‑CCCGGATCGAAAAGCCAg                                                                                                                  <  4:24455/149‑1 (MQ=255)                                          gTGCTGCCGGTGGGCGCGAAAGTGCGCTTCCTGGTCACTGCTGCCGATGTGATCCACTCCTGGTGGGTGCCGGCCTTCGCGGTCAAAC‑GCGACGCCATCCCAGGCTTTGTCAACGAAGCCTGGA‑CCCGGATCGAAAAGCCAGGCATCTa                                                                                                           >  5:210772/1‑149 (MQ=255)                                            gctgcCGGTGGGCGCGAAAGTGCGCTTCCTGGTCACTGCTGCCGATGTGATCCACTCCTGGTGGGTGCCGGCCTTCGCGGTCAAAC‑GCGACGCCATCCCAGGCTTCGTTGACAAAGCCTg                                                                                                                                       >  3:650/1‑120 (MQ=255)                                            gctgcCGGTGGGCGCGAAAGTGCGCTTCCTGGTCACTGCTGCCGATGTGATCCACTCCTGGTGGGTGCCGGCCTTCGCGGTCAAAC‑GCGACGCCATCCCAGGCTTCGTTGACAAAGCCTg                                                                                                                                       <  4:650/120‑1 (MQ=255)                                              tgcCGGTGGGCGCGAAAGTGCGCTTCCTGGTCACTGCTGCCGATGTGATCCACTCCTGGTGGGTGCCGGCCTTCGCGGTCAAAC‑GCGACGCCATCCCAGGCTTTGTCAACGAAGCCTGGA‑CCCGGATCGAAAAGCCAGGCATCTACcgc                                                                                                       >  6:330577/1‑149 (MQ=255)                                                   gTGGGCGCGAAAGTGCGCTTCCTGGTCACTGCTGCCGATGTGATCCACTCCTGGTGGGTGCCGGCCTTCGCGGTCAAAC‑GCGACGCCATCCCAGGCTTTGTCAACGAAGCCTGGA‑CCCGGATCGAAAAGCCAGGCATCTACCGCGGCCa                                                                                                  <  6:210772/149‑1 (MQ=255)                                                     gggCGCGAAAGTGCGCTTCCTGGTCACTGCTGCCGATGTGATCCACTCCTGGTGGGTGCCGGCCTTCGCGGTCAAAC‑GCGACGCCATCCCAGGCTTTGTCAACGAAGCCTGGA‑CCCGGATCGAAAAGCCAGGCATCTACCGCGGc                                                                                                    >  6:289765/1‑145 (MQ=255)                                                     gggCGCGAAAGTGCGCTTCCTGGTCACTGCTGCCGATGTGATCCACTCCTGGTGGGTGCCGGCCTTCGCGGTCAAAC‑GCGACGCCATCCCAGGCTTTGTCAACGAAGCCTGGA‑CCCGGATCGAAAAGCCAGGCATCTACCGCGGc                                                                                                    <  5:289765/145‑1 (MQ=255)                                                       gcgcGAAAGTGCGCTTCCTGGTCACTGCTGCCGATGTGATCCACTCCTGGTGGGTGCCGGCCTTCGCGGTCAAAC‑GCGCCGCCATCCCAGGCTTTGTCAACGAAGCCTGGA‑CCCGGAGCGAAAAGCCAGGCATCTACCGCGGCCAGTGc                                                                                              >  8:115736/1‑149 (MQ=255)                                                           gAAAGTGCGCTTCCTGGTCACTGCTGCCGATGTGATCCACTCCTGGTGGGTGCCGGCCTTCGCGGTCAAAC‑GCGACGCCATCCCAGGCTTTGTCAACGAAGCCTGGA‑CCCGGATCGAAAAGCCAGGCATCTACCGCGGCCAGTGCAcc                                                                                           >  1:152210/1‑148 (MQ=255)                                                                tGCGCTTCCTGGTCACTGCTGCCGATGTGATCCACTCCTGGTGGGTGCCGGCCTTCGCGGTCAAAC‑GCGACGCCATCCCAGGCTTTGTCAACGAAGCCTGGACCCCGGATCGAAAAGCCAGGCATCTACCGCGGCCAGTGCACCGAGCt                                                                                      <  1:72631/149‑1 (MQ=255)                                                                 cggcTTCCTGGGCCCTGCTGCGTATGTTTTCAATTCATGGGGGGTGCGGGCCTGCGGGTGAAAAC‑GCGACGCCATCCCAGGCTTTGTCAACGAAGCCTGGA‑CCCGGATCGAAAAGCCAGGCATCTACCGCGGCCAGTGCACCGAGCtgt                                                                                    <  2:66616/147‑1 (MQ=255)                                                                  ctcTTGCTGGTCACTGCTGCGTATTTGATCCACTCCTGGTGGGTGCCGGCCTTCGCGGTCAAAC‑GCGACGGCATCCCAGGCTTTGTCAACGTAGCCTGGA‑CCCGGATCGAAAAGCCAGGCATCTACCGCGGCCAGTGCACCGAGCtgt                                                                                    <  2:152210/146‑1 (MQ=255)                                                                     ttCCTGGTCACTGCTGCCGATGTGATCCACTCCTGGTGGGTGCCGGCCTTCGCGGTCAAAC‑GCGACGCCATCCCAGGCTTTGTCAACGAAGCCTGGA‑CCCGGATCGAAAAGCCAGGCATCTACCGCGGCCAGTGCACCGAGCTGTGCgg                                                                                >  6:49898/1‑149 (MQ=255)                                                                         tGGTCACTGCTGCCGATGTGATCCACTCCTGGTGGGTGCCGGCCTTCGCGGTCAAAC‑GCGACGCCATCCCAGGCTTTGTCAACGAAGCCTGGA‑CCCGGATCGAAAAGCCAGGCATCTACCGCGGCCAGTGCACCGAGCTGTGCGGCAAg                                                                            >  1:272258/1‑149 (MQ=255)                                                                           gTCACTGCTGCCGATGTGATCCACTCCTGGTGGGTGCCGGCCTTCGCGGTCAAAC‑GCGACGCCATCCCAGGCTTTGTCAACGAAGCCTGGA‑CCCGGATCGAAAAGCCAGGCATCTACCGCGGCCAGTGCACCGAGCTGTGCGGCAAGGa                                                                          <  5:293496/149‑1 (MQ=255)                                                                               ctgctgCCGATGTGATCCACTCCTGGTGGGTGCCGGCCTTCGCGGTAAAAC‑GCGACGCCATCCCAGGCTTTGTCAACGAAGCCTGGA‑CCCGGATCGAAAAGCCAGGCATCTACCGCGGACAGTGCACCGAGCTGTGCGGCAAGGAcaaa                                                                      >  8:135049/1‑146 (MQ=255)                                                                                ttatgcCGATGTGATCCACTCCTGGTGGGTGCCGGCCTTCGCGGTCAAAC‑GCGACGCCATCACATGCTTTGTCAACGAAGCCTGGA‑CCCGGATCGAAAAGCCAGGCATCTACCGCGGCCAGTGCACCGAGCTGTGCGGCAAGGACCACg                                                                     <  7:135049/146‑1 (MQ=255)                                                                                tgctgcCGATGTGATCCACTCCTGGTGGGTGCCGGCCTTCGCGGTCAAAC‑GCGACGCCATCCCAGGCTTTGTCAACGAAGCCTGGA‑CCCGGATCGAAAAGCCAGGCATCTACCGCGGCCAGTGCACCGAGCTGTGCGGCAAGGACCACg                                                                     >  5:235801/1‑149 (MQ=255)                                                                                   tgcCGATGTGATCCACTCCTGGTGGGTGCCGGCCTTCGCGGTCAAAC‑GCGACGCCATCCCAGGCTTTGTCAACGAAGCCTGGA‑CCCGGATCGAAAAGCCAGGCATCTACCGCGGCCAGTGCACCGAGCTGTGCGGCAAGGACCACGGCt                                                                  <  6:235801/149‑1 (MQ=255)                                                                                       gATGTGATCCACTCCTGGTGGGTGCCGGCCTTCGCGGTCAAAC‑GCGACGCCATCCCAGGCTTTGTCAACGAAGCCTGGA‑CCCGGATCGAAAAGCCAGGCATCTACCGCGGCCAGTGCACCGAGCTGTGCGGCAAGGACCACGGCTTCAt                                                              >  4:164614/1‑149 (MQ=255)                                                                                             aTCCACTCCTGGTGGGTGCCGGCCTTCGCGGTCAAAC‑GCGACGCCATCCCAGGCTTTGTCAACGAAGCCTGGA‑CCCGGATCGAAAAGCCAGGCATCTACCGCGGCCAGTGCACCGAGCTGTGCGGCAAGGACCACGGCTTCATGCCggt                                                        >  6:269941/1‑149 (MQ=255)                                                                                              tCCACTCCTGGTGGGTGCCGGCCTTCGCGGTCAAAC‑GCGACGCCATCCCAGGCTTTGTCAACGAAGCCTGGA‑CCCGGATCGAAAAGCCAGGCATCTACCGCGGCCAGTGCACCGAGCTGTGCGGCAAGGACCACGGCTTCATGCCggtg                                                       <  3:164614/149‑1 (MQ=255)                                                                                                gatctcTGGTGGGGTCCGGCCTTCGCGGTCAAAC‑GCGACGCCATCCCAGTCTTTGTCAACGAAGCCTGGA‑CCCGGATCGAAAAGCCAGGCATCTACCGCGGCCAGTGCACCGAGCTGTGCGGCAAGGACCACGGCTTCATGCCggtgg                                                      <  8:331160/143‑1 (MQ=255)                                                                                                 aCTCCTGGTGGGTGCCGGCCTTCGCGGTCAAACGGCGACGCCATCCAAGGCTTTGTCAACGAAGCCTGGA‑CCCGGATCGAAAAGCCAGGCATCTACCGCGGCCAGTGCACCGAGCTGTGCGGCAAGGACCACGGCTTCATGCCggtggt                                                     >  1:222977/1‑149 (MQ=255)                                                                                                   tCCTGGTGGGTGCCGGCCTTCGCGGTCAAAC‑GCGACGCCATCCCAGGCTTTGTCAACGAAGCCTGGA‑CCCGGATCGAAAAGCCAGGCATCTACCGCGGCCAGTGCACCGAGCTGTGCGGCAAGGACCACGGCTTCATGCCggtggtggt                                                  >  1:135124/1‑149 (MQ=255)                                                                                                      tggtggGTGCCGGCCTTCGCGGTCAAAC‑GCGACGCCATCCCAGGCTTTGTCAACGAAGCCTGTA‑CCCGGATCGAAAAGCCAGGCATCTACCGCGGCCAGTGCACCGAGCTGTGCGGCAAGGACCACGGATTCATGCCcgtgggagatc                                                >  7:331160/1‑142 (MQ=255)                                                                                                      tggtggGTGCCGGCCTTCGCGGTCAAAC‑GCGACGCCATCCCAGGCTTTGTCAACGAAGCCTGGA‑CCCGGATCGAAAAGCCAGGCATCTACCGCGGCCAGTGCACCGAGCTGTGCGGCAAGGACCACGGCTTCATGCCGGTGGTGGTc                                                 >  6:302894/1‑147 (MQ=255)                                                                                                      tggtggGTGCCGGCCTTCGCGGTCAAAC‑GCGACGCCATCCCAGGCTTTGTCAACGAAGCCTGGA‑CCCGGATCGAAAAGCCAGGCATCTACCGCGGCCACTGCACCGCGCTGTGCGGCAAGGACCACGGCTTCATGCCGGTGGTTGTc                                                 >  8:79425/1‑147 (MQ=255)                                                                                                           ggtgCCGGCCTTCGCGGTCAAAC‑GCGACGCCATCCCAGGCTTTGTCAACGAAGCCTGGA‑CCCGGATCGAAAAGCCAGGCATCTACCGCGGCCAGTGCAcc                                                                                           <  8:254087/100‑1 (MQ=255)                                                                                                           ggtgCCGGCCTTCGCGGTCAAAC‑GCGACGCCATCCCAGGCTTTGTCAACGAAGCCTGGA‑CCCGGATCGAAAAGCCAGGCATCTACCGCGGCCAGTGCAcc                                                                                           >  7:254087/1‑100 (MQ=255)                                                                                                            gtgCCGGCCTTCGCGGTCAAAC‑GCGACGCCATCCCAGGCTTTGTCAACGAAGCCTGGA‑CCCGGATCGAAAAGCCAGGCATCTACCGCGGCCAGTGCACCGAGCTGTGCGGCAAGGACCACGGCTTCATGCCGGTGGTGGTCGAGGTCaa                                         <  2:222977/149‑1 (MQ=255)                                                                                                                 ggCCTTCGCGGTCAAAC‑GCGACGCCATCCCAGGCTTTGTCAACGAAGCCTGGA‑CCCGGATCGAAAAGCCAGGCATCTACCGCGGCCAGTGCACCGAGCTGTGCGGCAAGGACCACGGCTTCATGCCGGTGGTGGTCGAGGTCAAGTCCa                                    <  5:269941/149‑1 (MQ=255)                                                                                                                     ttCGCGGTCAAAC‑GCGACGCCATCCCAGGCTTTGTCAACGAAGCCTGGA‑CCCGGATCGAAAAGCCAGGCATCTACCGCGGCCAGTGCACCGAGCTGTGCGGCaa                                                                             >  1:197312/1‑104 (MQ=255)                                                                                                                     ttCGCGGTCAAAC‑GCGACGCCATCCCAGGCTTTGTCAACGAAGCCTGGA‑CCCGGATCGAAAAGCCAGGCATCTACCGCGGCCAGTGCACCGAGCTGTGCGGCaa                                                                             <  2:197312/104‑1 (MQ=255)                                                                                                                     ttCGCGGTCAAAC‑GCGACGCCATCCCAGGCTTTGTCAACGAAGCCTGGA‑CCCGGATCGAAAAGCCAGGCATCTACCGCGGCCAGTGCACCGAGCTGTGCGGCAAGGACCACGGCTTCATGCCGGTGGTGGTCGAGGTCAAGTCCAAGGc                                >  6:21087/1‑149 (MQ=255)                                                                                                                     ttCGCGGTCAAAC‑GCGACGCCATCCCAGGCTTTGTCAACGAAGCCTGGA‑CCCGGATCGAAAAGCCAGGCATCTACCGCGGCCAGTGCACCGAGCTGTGCGGCAAGGACCACGGCTTCATGCCGGTGGTGGTCGAGGTCAAGTCCAAGGc                                >  6:300122/1‑149 (MQ=255)                                                                                                                     ttCGCGGTCAAAC‑GCGACGCCATCCCAGGCTTTGTCAACGAAGCCTGGA‑CCCGGATCGAAAAGCCAGGCATCTACCGCGGCCAGTGCACCGAGCTGTGCGGCAAGGACCACGGCTTCATGCCGGTGGTGGTCGAGGTCAAGTCCAAGGc                                >  4:150480/1‑149 (MQ=255)                                                                                                                       cgcgGTCAAAC‑GCGACGCCATCCCAGGCTTTGTCAACGAAGCCTGGA‑CCCGGATCGAAAAGCCAGGCATCTACCGCGGCCAGTGCATCGAGCTGTGCGGCAAGGACCACGGCTTCATGCCGGTGGTGGTCGAGGTCAAGTCCAAGGc                                <  3:315987/147‑1 (MQ=255)                                                                                                                       cgcgGTCAAAC‑GCGACGCCATCCCAGGCTTTGTCAACGAAGCCTGGA‑CCCGGATCGAAAAGCCAGGCATCTACCGCGGCCAGTGCACCGAGCTGTGCGGCAAGGACCACGGCTTCATGCCGGTGGTGGTCGAGGTCAAGTCCAAGGCg                               >  1:56899/1‑148 (MQ=255)                                                                                                                        gcgGTCAAAC‑GCGACGCCATCCCAGGCTTTGTCAACGAAGCCTGGA‑CCCGGATCGAAAAGCCAGGCATCTACCGCGGCCAGTGCACCGAGCTGTGCGGCAAGGACCACGGCTTCATGCCGGTGGTGGTCGAGGTCAAGTCCAAGGCGGa                             <  3:150480/149‑1 (MQ=255)                                                                                                                          ggTCAAAC‑GCGACGCCATCCCAGGCTTTGTCAACGAAGCCTGGA‑CCCGGATCGAAAAGCCAGGCATCTACCGCGGCCAGTGCACCGAGCTGTGCGGCAAGGACCACGGCTTCATGCCGGTGGTGGTCGAGGTCAAGTCCAAGGCGGAtt                           <  2:56899/149‑1 (MQ=255)                                                                                                                              aaaC‑GCGACGCCATCCCAGGCTTTGTCAACGAAGCCTGGA‑CCCGGATCGAAAAGCCAGGCATCTACCGCGGCCAGTGCACCGAGCTGTGCGGCAAGGACCACGGCTTCATGCCGGTGGTGGTCGAGGTCAAGTCCAAGGCGGATTACg                        >  2:265581/1‑148 (MQ=255)                                                                                                                              aaaC‑GCGACGCCATCCCAGGCTTTGTCAACGAAGCCTGGA‑CCCGGATCGAAAAGCCAGGCATCTACCGCGGCCAGTGCACCGAGCTGTGCGGCAAGGACCACGGCTTCATGCCGGTGGTGGTCGAGGTCAAGTCCAAGGCGGATTACGa                       >  5:117003/1‑149 (MQ=255)                                                                                                                                aC‑GCGACGCCATCCCAGGCTTTGTCAACGAAGCCTGGA‑CCCGGATCGAAAAGCCAGGCATCTACCGCGGCCAGTGCACCGAGCTGTGCGGCAAGGACCACGGCTTCATGCCGGTGGTGGTCGAGGTCAAGTCCAAGGCGGATTACGaca                     >  3:156075/1‑149 (MQ=255)                                                                                                                                aC‑GCGACGCCATCCCAGGCTTTGTCAACGAAGCCTGGA‑CCCGGATCGAAAAGCCAGGCATCTACCGCGGCCAGTGCACCGAGCTGTGCGGCAAGGACCACGGCTTCATGCCGGTGGTGGTCGAGGTCAAGTCCAAGGCGGATTACGaca                     >  8:179021/1‑149 (MQ=255)                                                                                                                                   gcgACGCCATCCCAGGCTTTGTCAACGAAGCCTGGA‑CCCGGATCGAAAAGCCAGGCATCTACCGCGGCCAGTGCACCGAGCTGTGCGGCAAGGACCACGGCTTCATGCCGGTGGTGGTCGAGGTCAAGTCCAAGGCGGATTACGACAcc                   >  4:287722/1‑149 (MQ=255)                                                                                                                                         ccATCCCAGGCTTTGTCAACGAAGCCTGGA‑CCCGGATCGAAAAGCCAGGCATCTACCGCGGCCAGTGCACCGAGCTGTGCGGCAAGGACCACGGCTTCATGCCGGTGGTGGTCGAGGTCAAGTCCAAGGCGGATTACGACACCTGGCtt             <  5:330577/149‑1 (MQ=255)                                                                                                                                           ggtccAGGCTTCGTTGACAAAGCCTGGA‑CCCGGATCGAAAAGCCAGGCATCTACCGCGGCCAGTGCACCGAGCTGTGCGGCAAGGACCACGGCTTCATGCCGGTGGTGGTCGAGGTCAAGTCCAAGGCGGATTACGACACCTGGCTTgg           >  3:182857/4‑149 (MQ=255)                                                                                                                                              ccAGGCTTTGTCAACGAAGCCTGGA‑CCCGGATCGAAAAGCCAGGCATCTACCGCGGCCAGTGCACCGAGCTGTGCGGCAAGGACCACGGCTTCATGCCGGTGGTGGTCGAGGTCAAGTCCAAGGCGGATTACGACACCTGGCTTGGc          >  4:175490/1‑147 (MQ=255)                                                                                                                                                aGGCTTTGTCAACGAAGCCTGGA‑CCCGGATCGAAAAGCCAGGCATCTACCGCGGCCAGTGCACCGAGCTGTGCGGCAAGGACCACGGCTTCATGCCGGTGGTGGTCGAGGTCAAGTCCAAGGCGGATTACGACACCTGGCTTGGCGAgc      >  6:152728/1‑149 (MQ=255)                                                                                                                                                  gCTTTGTCAACGAAGCCTGGA‑CCCGGATCGAAAAGCCAGGCATCTACCGAGGCCAGTGCACCGAGCTGTGCGGCAAGGACCACGGCTTCATGCCGGTGGTGGTCGAGGTCAAGTCCAAGGCGGATTACGACACCTGGCTTGGCGAgcg     >  5:237692/1‑148 (MQ=255)                                                                                                                                                      tGTCAACGAAGCCTGGA‑CCCGGATCGAAAAGCCAGGCATCTACCGCGGCCAGTGCACCGAGCTGTGCGGCAAGGACCACGGCTTCATGCCGGTGGTGGTCGAGGTCAAGTCCAAGGCGGATTACGACACCTGGCTTGGCGAGCGCaa  <  5:300122/147‑1 (MQ=255)                                                                                                                                                      tGTCAACGAAGCCTGGA‑CCCGGATCGAAAAGCCAGGCATCTACCGCGGCCAGTGCACCGAGCTGTGCGGCAAGGACCACGGCTTCATGCCGGTGGTGGTCGAGGTCAAGTCCAAGGCGGATTACGACACCTGGCTTGGCGAGCGCaa  <  5:21087/147‑1 (MQ=255)                                                                                                                                                      |                                                                                                                                                    CAAAGGATGAGCACTACCTGCTGGAAGTCGACCAGCCGCTGGTGCTGCCGGTGGGCGCGAAAGTGCGCTTCCTGGTCACTGCTGCCGATGTGATCCACTCCTGGTGGGTGCCGGCCTTCGCGGTCAAAC‑GCGACGCCATCCCAGGCTTTGTCAACGAAGCCTGGA‑CCCGGATCGAAAAGCCAGGCATCTACCGCGGCCAGTGCACCGAGCTGTGCGGCAAGGACCACGGCTTCATGCCGGTGGTGGTCGAGGTCAAGTCCAAGGCGGATTACGACACCTGGCTTGGCGAGCGCAA  >  NC_002947/109279‑109573

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑