Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	733,052	T→C	10.7%	intergenic (+27/‑336)	clpB → / → PP_t07	chaperone protein ClpB/tRNA‑Asn

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	733,052	0	T	C	10.7%	142.7 / 7.7	56	intergenic (+27/‑336)	clpB/PP_t07	chaperone protein ClpB/tRNA‑Asn
Reads supporting (aligned to +/- strand):  ref base T (23/27);  new base C (3/3);  total (26/30)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.83e-01

GCGTGCGATCCAGCGCTGGATCGAGAACCCGCTGGCGCAGCTGATTCTGGCCGGCAAGTTCCTGCCCGGTACGGCGATCACCGCCAAGGTGGAAGGCGACGAAATCGTCTTTGGCTGATTGTGTTGCACCATGAGCCCCGCGTTTGCGGGGCT‑TTTTTTGCCTGTTGCCAATGCCGGCACAGCCCAGCTATTTCGGGCCTTTGGGCCAACCCGGGGTAACCGCCTGAAATTTTTGAAATTTTTGCTTGCATCAAATTTAGAGTGCCCCTAATATACGCCGCGTTGTCA  >  NC_002947/732908‑733195                                                                                                                                                 |                                                                                                                                                 gCGTGCGATCCAGCGCTGGATCGAGAACCCGCTGGCGCAGCTGATTCTGGCCGGCAAGTTCCTGCCCGGTACGGCGATCACCGCCAAGGTGGAAGGCGACGAAATCGTCTTTGGCTGATTGTGTTGCACCATGAGCCCCGCGTTTGCgg                                                                                                                                              <  6:5464/149‑1 (MQ=255)  cGTGCGATCCAGCGCTGGATCGAGAACCCGCTGGCGCAGCTGATTCTGGCCGGCAAGTTCCTGCCCGGTACGGCGATCACCGCCAAGGTGGAAGGCGACGAAATCGTCTTTGGCTGATTGTGTTGCACCATGAGCCCCGCGTTTGCggg                                                                                                                                             <  6:12079/149‑1 (MQ=255)     gCGATCCAGCGCTGGATCGAGAACCCGCTGGCGCAGCTGATTCTGGCCGGCAAGTTCCTGCCCGGTACGGCGATCACCGCCAAGGTGGAAGGCGACGAAATCGTCTTTGGCTGATTGTGTTGCACCATGAGCCCCGCGTTTGCGGGGCt                                                                                                                                          >  7:122595/1‑149 (MQ=255)       gATCCAGCGCTGGATCGAGAACCCGCTGGCGCAGCTGATTCTGGCCGGCAAGTTCCTGCCCGGTACGGCGATCACCGCCAAGGTGGAAGGCGACGAAATCGTCTTTGGCTGATTGTGTTGCACCATGAGCCCCGCAAACGCGGGGCtca                                                                                                                                        >  2:127218/1‑147 (MQ=255)        aTCCAGCGCTGGATCGAGAACCCGCTGGCGCAGCTGATTCTGGCCGGCAAGTTCCTGCCCGGTACGGCGATCACCGCCAAGGTGGAAGGCGACGAAATCGTCTTTGGCTGATTGTGTTGCACCATGAGCCCCGCAAACGCGGGGCtcat                                                                                                                                       <  1:127218/149‑4 (MQ=255)            aGCGCTGGATCGAGAACCCGCTGGCGCAGCTGATTCTGGCCGGCAAGTTCCTGCCCGGTACGGCGATCACCGCCAAGGTGGAAGGCGACGAAATCGTCTTTGGCTGATTGTGTTGCACCATGAGCCCCGCGTTTGCGGGGCT‑TTTTTTg                                                                                                                                  <  7:143297/149‑1 (MQ=255)             gcgcTGGATCGAGAACCCGCTGGCGCAGCTGATTCTGGCCGGCAAGTTCCTGCCCGGTACGGCGATCACCGCCAAGGTGGAAGGCGACGAAATCGTCTTTGGCTGATTGTGTTGCACCATGAGCCCCGCGTTTGCGGGGCT‑TTTTTTGc                                                                                                                                 <  2:67470/149‑1 (MQ=255)                     tCGAGAACCCGCTGGCGCAGCTGATTCTGGCCGGCAAGTTCCTGCCCGGTACGGCGATCACCGCCAAGGTGGAAGGCGACGAAATCGTCTTTGGCTGATTGTGTTGCACCATGAGCCCCGCGTTTGCGGGGCT‑TTTTTTGCCTGTTGc                                                                                                                          >  5:254662/1‑148 (MQ=255)                       gagaACCCGCTGGCGCAGCTGATTCTGGCCGGCAAGTTCCTGCCCGGTACGGCGATCACCGCCAAGGTGGAAGGCGACGAAATCGTCTTTGGCTGATTGTGTTGCACCATAAGCCCCGCAAACGCGGGGCtcat                                                                                                                                       <  7:45763/134‑4 (MQ=255)                       gagaACCCGCTGGCGCAGCTGATTCTGGCCGGCAAGTTCCTGCCCGGTACGGCGATCACCGCCAAGGTGGAAGGCGACGAAATCGTCTTTGGCTGATTGTGTTGCACCATAAGCCCCGCAAACGCGGGGCtcat                                                                                                                                       >  8:45763/1‑131 (MQ=255)                          aaCCCGCTGGCGCAGCTGATTCTGGCCGGCAAGTTCCTGCCCGGTACGGCGATCACCGCCAAGGTGGAAGGCGACGAAATCGTCTTTGGCTGATTGTGTTGCACCATGAGCCCCGCGTTTGCGGGGCT‑TTTTTTGCCTGTTGCCAATGc                                                                                                                    >  5:58205/1‑149 (MQ=255)                           aCCCGCT‑GCGCAGCTGATTCTGGCCGGCAAGTTCCTGCCCGGTACGGCGATCACCGCCAAGGTGGAAGGCGACGAAATCGTCTTTGGCTGATTGTGTTGCACCATGAGCCCCGCGTTTGCGGGGCT‑TTTTTTGCCTGTTGCCAATGCCg                                                                                                                  >  2:87182/1‑149 (MQ=255)                                   gcgcAGCTGATTCTGGCCGGCAAGTTCCTGCCCGGTACGGCGATCACCGCCAAGGTGGAAGGCGACGAAATCGTCTTTGGCTGATTGTGTTGCACCATGAGCCCCGCGTTTGCGGGGCT‑TTTTTTGCCTGTTGCCAATGCCGGCACAGc                                                                                                           >  3:344696/1‑149 (MQ=255)                                      cAGCTGATTCTGGCCGGCAAGTTCCTGCCCGGTACGGCGATCACCGCCAAGGTGGAAGGCGACGAAATCGTCTTTGGCTGATTGTGTTGCACCATGAGCCCCGCGTTTGCGGGGCT‑TTTTTTGCCTGTTGCCAATGCCGGCACAGCCCa                                                                                                        <  7:209420/149‑1 (MQ=255)                                      cAGCTGATTCTGGCCGGCAAGTTCCTGCCCGGTACGGCGATCACCGCCAAGGTGGAAGGCGACGAAATCGTCTTTGGCTGATTGTGTTGCACCATGAGCCCCGCGTTTGCGGGGCT‑TTTTTTGCCTGTTGCCAATGCCGGCACAGCCCa                                                                                                        <  6:254662/149‑1 (MQ=255)                                            aTTCTGGCCGGCAAGTTCCTGCCCGGTACGGCGATCACCGCCAAGGTGGAAGGCGACGAAATCGTCTTTGGCTGATTGTGTTGCACCATGAGCCCCGCGTTTGCGGGGCT‑TTTTTTGCCTGTTGCCAATGCCGGCACAGCCCAGCTAtt                                                                                                  >  8:175267/1‑149 (MQ=255)                                                        aaGTTCCTGCCCGGTACGGCGATCACCGCCAAGGTGGAAGGCGACGAAATCGTCTTTGGCTGATTGTGTTGCACCATGAGCCCCGCGTTTGCGGGGCT‑TTTTTTGCCTGTTGCCAATGCCGGCACAGCCCAGCTATTTCGGGCCTTTgg                                                                                      <  1:150991/149‑1 (MQ=255)                                                         aGTTCCTGCCCGGTACGGCGATCACCGCCAAGGTGGAAGGCGACGAAATCGTCTTTGGCTGATTGTGTTGCACCATGAGCCCCGCGTTTGCGGGGCTCTTTTTTGCCTGTTGCCAATGCCGGCACAGCCCAGCTATTTCGGGCCTTTgg                                                                                      <  1:87182/149‑1 (MQ=255)                                                             ccTGCCCGGTACGGCGATCACCGCCAAGGTGGAAGGCGACGAAATAGTAGTTTGCTGATTTTGTTGCACCATTAGCCCCGCGGTTTCGGGGCT‑TTTTTTGCCAGTTTCCAATTCCGGCACAGCCCAGCTATTTACGGCCGTTTGGCCaa                                                                                 >  1:243605/1‑149 (MQ=255)                                                                   cGGTACGGCGATCACCGCCAAGGTGGAAGGCGACGAAATCGTCTTTGGCTGATTGTGTTGCACCATGAGCCCCGCGTTTGCGGGGCT‑TTTTTTGCCTGTTGCCAATGCCGGCACAGCCCAGCTATTTCGGGCCTTTGGGCCAACCCggg                                                                           <  4:344696/149‑1 (MQ=255)                                                                        cGGCGATCACCGCCAAGGTGGAAGGCGACGAAATCGTCTTTGGCTGATTGTGTTGCACCATGAGCCCCGCGTTTGCGGGGCT‑TTTTTTGCCTGTTGCCAATGCCGGCACAGCCCAGCTATTTCGGGCCTTTGGGCCAACCCGGGGTaa                                                                       <  8:151948/148‑1 (MQ=255)                                                                            gATCACCGCCAAGGTGGAAGGCGACGAAATCGTCTTTGGCTGATTGTGTTGCACCATGAGCCCCGCGTTTGCGGGGCT‑TTTTTTGCCTGTTGCCAATGCCGGCACAGCCCAGCTAt                                                                                                   <  8:298422/116‑1 (MQ=255)                                                                            gATCACCGCCAAGGTGGAAGGCGACGAAATCGTCTTTGGCTGATTGTGTTGCACCATGAGCCCCGCGTTTGCGGGGCT‑TTTTTTGCCTGTTGCCAATGCCGGCACAGCCCAGCTAt                                                                                                   >  7:298422/1‑116 (MQ=255)                                                                              tCACCGCCAAGGTGGAAGGCGACGAAATCGTCTTTGGCTGATTGTGTTGCACCATGAGCCCCGCAAACGCGGGGCtca                                                                                                                                        >  5:338780/1‑76 (MQ=38)                                                                              tCACCGCCAAGGTGGAAGGCGACGAAATCGTCTTTGGCTGATTGTGTTGCACCATGAGCCCCGCAAACGCGGGGCtca                                                                                                                                        <  6:338780/78‑3 (MQ=38)                                                                              tCACCGCCAAGGTGGAAGGCGACGAAATCGTCTATGGCTGATTGTGTTGCACCATGAGCCCCGCGTTTGCGGGGCT‑TTTTTTGCCTGTTGCCAATGCCGGCACAGCCAAGCTATTACGGGCCTTTGGGCCAACCCGGGGTAACCGCCt                                                                 >  1:232731/1‑148 (MQ=255)                                                                                aCCGCCAAGGTGGAAGGCGACGAAATCGTCTTTGGCTGATTGTGTTGCACCATGAGCCCCGCGTTTGCGGGGCT‑TTTTTTGCCTGTTGCCAATGCCGGCACAGCCCAGCTATTTCGGGCCTTTGGGCCAACCCGGGGTAACCGCCtga                                                               <  6:58205/148‑1 (MQ=255)                                                                                 ccGCCAAGGTGGAAGGCGACGAAATCGTCTTTGGCTGATTGTGTTGCACCATGAGCCCCGCGTTTGCGGGGCT‑TTTTTTGCCTGTTGCCAATGCCGGCACAGCCCAGCTATTTCGGGCCTTTGGGCCAACCCGGGGTAACCGCCtgaaa                                                             >  6:314415/1‑149 (MQ=255)                                                                                  cGCCAAGGTGGAAGGCGACGAAATCGTCTTTGGCTGATTGTGTTGCACCATGAGCCCCGCGTTTGCGGGGCT‑TTTTTTGCCTGTTGCCAATGcc                                                                                                                   <  1:238405/94‑1 (MQ=255)                                                                                  cGCCAAGGTGGAAGGCGACGAAATCGTCTTTGGCTGATTGTGTTGCACCATGAGCCCCGCGTTTGCGGGGCT‑TTTTTTGCCTGTTGCCAATGcc                                                                                                                   >  2:238405/1‑94 (MQ=255)                                                                                  cGCCAAGGTGGAAGGCGACGAAATCGTCTTTGGCTGATTGTGTTGCACCATGAGCCCCGCGTTTGCGGGGCT‑TTTTTTGCCTGTTGCCAATGCCGGCACAGccc                                                                                                         >  6:79979/1‑104 (MQ=255)                                                                                  cGCCAAGGTGGAAGGCGACGAAATCGTCTTTGGCTGATTGTGTTGCACCATGAGCCCCGCGTTTGCGGGGCT‑TTTTTTGCCTGTTGCCAATGCCGGCACAGccc                                                                                                         <  5:79979/104‑1 (MQ=255)                                                                                  cGCCAAGGTGGAAGGCGACGAAATCGTCTTTGGCTGATTGTGTTGCACCATGAGCCCCGCGTTTGCGGGGCT‑TTTTTTGCCTGTTGCCAATGCCGGCACAGCCCAGCTATTTCGGGCCTTTGGGCCAACCCGGGGTAACCGCCtgaaat                                                            >  5:33181/1‑149 (MQ=255)                                                                                      aaGGTGGAAGGCGACGAAATCGTCTTTGGCTGATTGTGTTGCACCATGAGCCCCGCGTTTGCGGGGCT‑TTTTTTGCCTGTTGCCAATGCCGGCACAGCCCAGCTATTTCGGGCCTTTGGGCCAACCCGGGGTAACCGCCtgaaattttt                                                        <  5:314415/149‑1 (MQ=255)                                                                                                  gacgaAATCGTCTTTGGCTGATTGTGTTGCACCATGAGCCCCGCGTTTGCGGGGCT‑TTTTTTGCCTGTTGCCAATGCCGGCACAGCCCAGCTATTTCGGGCCTTTGGGCCAACCCGGGGTAACCGCCTGAAATTTTTGAAATTTttgct                                            <  7:175267/149‑1 (MQ=255)                                                                                                    cgaAATCGTCTTTGGCTGATTGTGTTGCACCATGAGCCCCGCGTTTGCGGGGCT‑TTTTTTGCCTGTTGCCAATGCCGGCACAGCCCAGCTATTTCGGGCCTTTGGGCCAACCCGGGGTAACCGCCTGAAATTTTTGAAATTTttgcttg                                          <  6:33181/149‑1 (MQ=255)                                                                                                              tttGGCTGATTGTGTTGCACCATGAGCCCCGCGTTTGCGGGGCT‑TTTTTTGCCTGTTGCCAATGCCGGCACAGCCCAGCTATTTCGGGCCTTTGGGCCAACCCGGGGTAACCGCCTGAAATTTTTGAAATTTTTGCTTGCATCAAAtt                                 >  2:36039/1‑148 (MQ=255)                                                                                                                tGGCTGATTGTGTTGCACCATGAGCCCCGCGTTTGCGGGGCT‑TTTTTTGCCTGTTGCCAATGCCGGCACAGCCCAGCTATTTCGGGCCTTTGGGCCAACCCGGGGTAACCGCCTGAAATTTTTGAAATTTTTGCTTGCATCAAAtt                                 >  2:229579/1‑146 (MQ=255)                                                                                                                tGGCTGATTGTGTTGCACCATGAGCCCCGCGTTTGCGGGGCT‑TTTTTTGCCTGTTGCCAATGCCGGCACAGCCCAGCTATTTCGGGCCTTTGGGCCAACCCGGGGTAACCGCCTGAAATTTTTGAAATTTTTGCTTGCATCAAATTTa                               <  2:243605/148‑1 (MQ=255)                                                                                                                     gATTGTGTTGCACCATGAGCCCCGCGTTTGCGGGGCT‑TTTTTTGCCTGTTGCCAATGCCGGCACAGCCCAGCTATTTCGGGCCTTTGGGCCAACCCGGGGTAACCGCCTGAAATTTTTGAAATTTttgcttgc                                         <  6:350081/133‑1 (MQ=255)                                                                                                                     gATTGTGTTGCACCATGAGCCCCGCGTTTGCGGGGCT‑TTTTTTGCCTGTTGCCAATGCCGGCACAGCCCAGCTATTTCGGGCCTTTGGGCCAACCCGGGGTAACCGCCTGAAATTTTTGAAATTTttgcttgc                                         >  5:350081/1‑133 (MQ=255)                                                                                                                           gtTGCACCATGAGCCCCGCGTTTGCGGGGCT‑TTTTTTGCCTGTTGCCAATGCCGGCACAGCCCAGCTATTTCGGGCCTTTGGGCCAACCCGGGGTAACCGCCTGAAATTTTTGAAATTTTTGCTTGAATCAAATTTAGAGTGCCCCTaa                   >  5:150913/1‑149 (MQ=255)                                                                                                                               cACCATGAGCCCCGCGTTTGCGGGGCT‑TTTTTTGCCTGTTGCCAATGCCGGCACAGCCCAGCTATTTCGGGCCTTTGGGCCAACCCGGGGTAACCGCCTGAAATTTTTGAAATTTTTGCTTGCATCAAATTTAGAGTGCCCCTAAtata               <  1:229579/149‑1 (MQ=255)                                                                                                                                aCCATGAGCCCCGCGTTTGCGGGGCT‑TTTTTTGCCTGTTGCCAATGCCGGCACAGCCCAGCTATTTCGGGCCTTTGGGCCAACCCGGGGTAACCGCCTGAAATTTTTGAAATTTTTGCTTGCATCAAATTTAGAGt                           <  5:40601/136‑1 (MQ=255)                                                                                                                                aCCATGAGCCCCGCGTTTGCGGGGCT‑TTTTTTGCCTGTTGCCAATGCCGGCACAGCCCAGCTATTTCGGGCCTTTGGGCCAACCCGGGGTAACCGCCTGAAATTTTTGAAATTTTTGCTTGCATCAAATTTAGAGt                           >  6:40601/1‑136 (MQ=255)                                                                                                                                aCCATGAGCCCCGCGTTTGCGGGGCT‑TTTTTTGCCTGTTGCCAATGCCGGCACAGCCCAGCTATTTCGGGCCTTTGGGCCAACCCGGGGTAACCGCCTGAAATTTTTGAAATTTTTGCTTGCATCAAATTTAGAGTGCCCCTAATATAc              <  1:36039/149‑1 (MQ=255)                                                                                                                                  cATGAGCCCCGCGTTTGCGGGGCT‑TTTTTTGCCTGTTGCCAATGCCGGCACAGCCCAGCTATTTCGGGCCTTTGGGCCAACCCGGGGTAACCGCCTGAAATTTTTGAAATTTTTGCTTGCATCAAATTTAGAGTGCCCCTAATATAcgc            >  1:328815/1‑149 (MQ=255)                                                                                                                                  cATGAGCCCCGCGTTTGCGGGGCT‑TTTTTTGCCTGTTGCCAATGCCGGCACAGCCCAGCTATTTCGGGCCTTTGGGCCAACCCGGGGTAACCGCCTGAAATTTTTGAAATTTTTGCTTGCATCAAATTTAGAGTGCCCCTAATATAcgc            <  2:328815/149‑1 (MQ=255)                                                                                                                                        ccccGCGTTTTCGGGGCT‑TTTTTTGCCTGTTGCCAATGCCGGCACAGCCCAGCTATTTCGGGCCTTTGGGCCAACCCGGGGTAACCGCCTGAAATTTTTGAAATTTTTGCTTGCATCAAATTTAGAGTGCCCCTAATATACGCCGCGtt      <  4:37901/149‑1 (MQ=255)                                                                                                                                          ccGCGTTTGCGGGGCT‑TTTTTTGCCTGTTGCCAATGCCGGCACAGCCCAGCTATTTCGGGCCTTTGGGCCAACCCGGGGTAACCGCCTGAAATTTTTGAAATTTTTGCTTGCATCAAATTTAGAGTGCCCCTAATATACGCCGCGTTGt    >  6:112541/1‑149 (MQ=255)                                                                                                                                          ccGCGTTTGCGGGGCT‑TTTTTTGCCTGTTGCCAATGCCGGCACAGCCCAGCTATTTCGGGCCTTTGGGCCAACCCGGGGTAACCGCCTGAAATTTTTGAAATTTTTGCTTGCATCAAATTTAGAGTGCCCCTAATATACGCCGCGTTGt    >  3:293882/1‑149 (MQ=255)                                                                                                                                          ccGCGTTTGCGGGGCT‑TTTTTTGCCCGTTGCCAATGCCGGCACAGCCCAGCTATTTCGGGCCTTTGGGCCAACCCGGGGTAACCGCCggaaattttt                                                        <  7:264097/97‑1 (MQ=255)                                                                                                                                          ccGCGTTTGCGGGGCT‑TTTTTTGCCCGTTGCCAATGCCGGCACAGCCCAGCTATTTCGGGCCTTTGGGCCAACCCGGGGTAACCGCCggaaattttt                                                        >  8:264097/1‑97 (MQ=255)                                                                                                                                           cgcgTTTGCGGGGCT‑TTTTTTGCCTGTTGCCAATGCCGGCACAGCCCAGCTATTTCGGGCCTTTGGGCCAACCCGGGGTAACCGCCTGAAATTTTTGAAATTTTTGCTTGCATCAAATTTAGAGTGc                         >  5:114212/1‑127 (MQ=255)                                                                                                                                           cgcgTTTGCGGGGCT‑TTTTTTGCCTGTTGCCAATGCCGGCACAGCCCAGCTATTTCGGGCCTTTGGGCCAACCCGGGGTAACCGCCTGAAATTTTTGAAATTTTTGCTTGCATCAAATTTAGAGTGc                         <  6:114212/127‑1 (MQ=255)                                                                                                                                            gcgTTTGCGGGGCT‑TTTTTTGCCTGTTGCCAATGCCGGCACAGCCCAGCTATTTCGGGCCTTTGGGCCAACCCGGGGTAACCGCCTGAAATTTTTGAAATTTTTGCTTGCATCAAATTTAGAGTGCCCCTAATATACGCCGCGTTGTCa  <  5:112541/149‑1 (MQ=255)                                                                                                                                                 |                                                                                                                                                 GCGTGCGATCCAGCGCTGGATCGAGAACCCGCTGGCGCAGCTGATTCTGGCCGGCAAGTTCCTGCCCGGTACGGCGATCACCGCCAAGGTGGAAGGCGACGAAATCGTCTTTGGCTGATTGTGTTGCACCATGAGCCCCGCGTTTGCGGGGCT‑TTTTTTGCCTGTTGCCAATGCCGGCACAGCCCAGCTATTTCGGGCCTTTGGGCCAACCCGGGGTAACCGCCTGAAATTTTTGAAATTTTTGCTTGCATCAAATTTAGAGTGCCCCTAATATACGCCGCGTTGTCA  >  NC_002947/732908‑733195

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑