Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 735,632 G→T 7.1% intergenic (‑58/+75) PP_0627 ← / ← PP_0628 hypothetical protein/hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_002947735,6320GT 7.1% 174.6 / 2.5 56intergenic (‑58/+75)PP_0627/PP_0628hypothetical protein/hypothetical protein
Reads supporting (aligned to +/- strand):  ref base G (26/26);  new base T (2/2);  total (28/28)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.06e-01

GCCGGAAGGGCCTGCGCTCGACTTGGTGCTGGGGGGCTCGCAGATACTCATCGACACGACGCTGATCTTCCGGGTTCAGGCGACTGGTCATGGTGGGCCTCGTCAGGTAGACGTTTGTGACTGCGCAAACGCTACAGCGTTCGCGCAGGCGCTTGAAACAAATGATAATGCTTTCTATCCCTGGCGCCGAGTGTACGCCATCGCAAACAGTCGCTGCACTGGATCAAAGACTGATGCCTACGTCGAAGACGATGCTGCGGCCCAGGTTGCCGCGCAGGAAATCCGGCGC  >  NC_002947/735484‑735772
                                                                                                                                                    |                                                                                                                                            
gCCGGAAGGGCCTGCGCTCGACTTGGTGCTGGGGGGCTCGCAGATACTCATCGACACGACGCTGATCTTCCGGGTTCAGGCGACTGGTCATGGTGGGCCTCGTCAGGTAGACGTTTGTGACTGCGCAAACGCTACAGCGTTCGCGCAgg                                                                                                                                              <  3:344321/149‑1 (MQ=255)
    gAAGGGCCTGCGCTCGACTTGGTGCTGGGGGGCTCGCAGATACTCATCGACACGACGCTGATCTTCCGGGTTCAGGCGACTGGTCATGGTGGGCCTCGTCAGGTAGACGTTTGTGACTGCGCAAACGCTACAGCGTTCGCGCAGGCGCt                                                                                                                                          >  4:146827/1‑149 (MQ=255)
           cTGCGCTCGACTTGGTGCTGGGGGGCTCGCAGATACTCATCGACACGACGCTGATCTTCCGGGTTCAGGCGACTGGTCATGGTGGGCCTCGTCAGGTAGACGTTTGTGACTGCGCAAACGCTACAGCGTTCGCGCAGGCGCTTGAAAc                                                                                                                                    >  8:63728/1‑148 (MQ=255)
           cTGCGCTCGACTTGGTGCTGGGGGGCTCGCAGATACTCATCGACACGACGCTGATCTTCCGGGTTCAGGCGACTGGTCATGGTGGGCCTCGTCAGGTAGACGTTTGTGACTGCGCAAACGCTACAGCGTTCGCGCAGGCGCTTGAAAc                                                                                                                                    <  7:63728/148‑1 (MQ=255)
             gcgcTCGACTTGGTGCTGGGGGGCTCGCAGATACTCATCGACACGACGCTGATCTTCCGGGTTCAGGCGACTGGTCATGGTGGGCCTCGTCAGGTAGACGTTTGTGACTGCGCAAACGCTACAGCGTTCGCGCAGGCGCTTGAAACaaa                                                                                                                                 <  6:189891/149‑1 (MQ=255)
              cgcTCGACTTGGTGCTGGGGGGCTCGCAGATACTCATCGACACGACGCTGATCTTCCGGGTTCAGGCGACTGGTCATGGTGGGCCTCGTCAGGTAGACGTTTGTGACTGCGCAAACGCTACAGCGTTCGCGCAGGCGCTTGAAACAAAt                                                                                                                                <  6:183762/149‑1 (MQ=255)
               gcTCGACTTGGTGCTGGGGGGCTCGCAGATACTCATCGACACGACGCTGATCTTCCGGGTTCAGGCGACTGGTCATGGTGGGCCTCGTCAGGTAGACGTTTGTGACTGCGCAAACGCTACAGCGTTCGCGCAGGCGCTTGAAACAAAt                                                                                                                                >  4:337663/1‑148 (MQ=255)
                    aCTTGGTGCTGGGGGGCTCGCAGATACTCATCGACACGACGCTGATCTTCCGGGTTCAGGCGACTGGTCATGGTGGGCCTCGTCAGGTAGACGTTTGTGACTGCGCAAACGCTACAGCGTTCGCGCAGGCGCTTGAAACAAATGATAAt                                                                                                                          <  3:337663/149‑1 (MQ=255)
                          tGCTGGGGGGCTCGCAGATACTCATCGACACGACGCTGATCTTCCGGGTTCAGGCGACTGGTCATGGTGGGCCTCGTCAGGTAGACGTTTGTGACTGCGCAAACGCTACAGCGTTCGCGCAGGCGCTTGAAACAAATGATAATGCTTTc                                                                                                                    >  1:240523/1‑149 (MQ=255)
                                   gCTCGCAGATACTCATCGACACGACGCTGATCTTCCGGGTTCAGGCGACTGGTCATGGTGGGCCTCGTCAGGTAGACGTTTGTGACTGCGCAAACGCTACAGCGTTCGCGCAGGCGCTTGAAACAAATGATAATGCTTTCTATCCCTgg                                                                                                           >  5:265678/1‑149 (MQ=255)
                                         aGATACTCATCGACACGACGCTGATCTTCCGGGTTCAGGCGACTGGTCATGGTGGGCCTCGTCAGGTAGACGTTTGTGACTGCGCAAACGCTACAGCGTTCGCGCAGGCGCTTGAAACAAATGATa                                                                                                                            <  1:308444/126‑1 (MQ=255)
                                         aGATACTCATCGACACGACGCTGATCTTCCGGGTTCAGGCGACTGGTCATGGTGGGCCTCGTCAGGTAGACGTTTGTGACTGCGCAAACGCTACAGCGTTCGCGCAGGCGCTTGAAACAAATGATa                                                                                                                            >  2:308444/1‑126 (MQ=255)
                                         aGATACTCATCGACACGACGCTGATCTTCCGGGTTCAGGCGACTGGTCATGGTGGGCCTCGTCAGGTAGACGTTTGTGACTGCGCAAACGCTACAGCGTTCGCGCAGGCGCTTGAAACAAATGATAATGCTTTCTATCCCTGGCGCCGa                                                                                                     <  2:240523/149‑1 (MQ=255)
                                         aGATACTCATCGACACGACGCTGATCTTCCGGGTTCAGGCGACTGGTCATGGTGGGCCTCGTCAGGTAGACGTTTGTGACTGCGCAAACGCTACAGCGTTCGCGCAGGCGCTTGAAACAAATGATAATGCTTTCTATCCCTGGCGCCGa                                                                                                     <  6:265678/149‑1 (MQ=255)
                                           aTACTCATCGACCCGACGCTGATCTTCCGGGTTCAGGCGACTGGTCATGGTGGGCCTCGTCAGGTAGACGTTTGTGACTGCGCAAACGCTACAGCGTTCGCGCAGGCGCTTGAAACAAATGATAATGCTTTCTATCCCTGGCGCCGAgt                                                                                                   <  2:238073/149‑1 (MQ=255)
                                               tCATCGACACGACGCTGATCTTCCGGGTTCAGGCGACTGGTCATGGTGGGCCTCGTCAGGTAGACGTTTGTGACTGCGCAAACGCTACAGCGTTCGCGCAGGCGCTTGAAACAAATGATAATGCTTTCTATCCCTGGCGCCGAGTGTAc                                                                                               >  1:200187/1‑149 (MQ=255)
                                               gcATCGACACGACGCTGATCTTCCGGGTTCAGGCGACTGGTCATGGTGGGCCTCGTCAGGTAGACGTTTGTGACTGCGCAAACGCTACAGCGTTCGCGCAGGCGCTTGAAACAAATGATAATGCTTTCTATCCCTGGCGCCGAGTGTAc                                                                                               <  2:148549/148‑1 (MQ=255)
                                                 aTCGACACGACGCTGATCTTCCGGGTTCAGGCGACTGGTCATGGTGGGCCTCGTCAGGTAGACGTTTGTCACTGCGCAAACGCTACAGCGTACGCGCAGGCGCTTGAAACAAATGATAATGCTTTCTATCCCTGGCGCCGAGTGTACGc                                                                                             >  7:334005/1‑149 (MQ=255)
                                                  tCGACACGACGCTGATCTTCCGGGTTCAGGCGACTGGTCATGGTGGGCCTCGTCAGGTAGACGTTTGTGACTGCGCAAACGCTACAGCGTTCGCGCAGGCGCTTGAAACAAATGATAATGCTTTCTATCCCTGGCGCCGAGTGTACGc                                                                                             >  6:187859/1‑148 (MQ=255)
                                                     acacGACGCTGATCTTCCGGGTTCAGGCGACTGGTCATGGTGGGCCTCGTCAGGTAGACGTTTGTGACTGCGCAAACGCTACAGCGTTCGCGCAGGCGCTTGAAACAAATGATAATGCTTTCTATCCCTGGCGCCGAGTGTACGCCATc                                                                                         <  4:319300/149‑1 (MQ=255)
                                                              tGATCTTCCGGGTTCAGGCGACTGGTCATGGTGGGCCTCGTCAGGTAGACGTTTGTGACTGCGCAAACGCTACAGCGTTCGCGCAGGCGCTTGAAACAAATGATAATGCTTTCTATCCCT‑GCGCCGAGTGTACGCCATCGCAAACAGTc                                                                               >  7:332410/1‑149 (MQ=255)
                                                                aTCTTCCGGGTTCAGGCGACTGGTCATGGTGGGCCTCGTCAGGTAGACGTTTGTGACTGCGCAAACGCTACAGCGTTCGCGCAGGCGCTTGAAACAAATGATAATGCttt                                                                                                                     >  8:116162/1‑110 (MQ=255)
                                                                aTCTTCCGGGTTCAGGCGACTGGTCATGGTGGGCCTCGTCAGGTAGACGTTTGTGACTGCGCAAACGCTACAGCGTTCGCGCAGGCGCTTGAAACAAATGATAATGCttt                                                                                                                     <  7:116162/110‑1 (MQ=255)
                                                                   ttCCGGGTTCAGGCGACTGGTCATGGTGGGCCTCGTCAGGTAGACGTTTGTGACTGCGCAAACGCTACAGCGTTCGCGCAGGCGCTTGAAACAAATGATAATGCTTTCTATCCCTGGCGCCGAGTGTACGCCATCGCAAACAGTCGCTg                                                                           >  7:325033/1‑149 (MQ=255)
                                                                       gggTTCAGGCGACTGGTCATGGTGGGCCTCGTCAGGTAGACGTTTGTGACTGCGCAAACGCTACAGCGTTCGCGCAGGCGCTTGAAACAAATGATAATGCTTTCTATCCCTGGCGCCGAGTGTACGCCATCGCAAACAGTCGCTGCACt                                                                       <  2:257193/149‑1 (MQ=255)
                                                                        ggTTCAGGCGACTGGTCATGGTGGGCCTCGTCAGGTAGACGTTTGTGACTGCGCAAACGCTACAGCGTTCGCGCAGGCGCTTGAAACAAATGATAATGCtt                                                                                                                      >  6:305073/1‑101 (MQ=255)
                                                                        ggTTCAGGCGACTGGTCATGGTGGGCCTCGTCAGGTAGACGTTTGTGACTGCGCAAACGCTACAGCGTTCGCGCAGGCGCTTGAAACAAATGATAATGCtt                                                                                                                      <  5:305073/101‑1 (MQ=255)
                                                                          ttCAGGCGACTGGTCATGGTGGGCCTCGTCAGGTAGACGTTTGTGACTGCGCAAACGCTACAGCGTTCGCGCAGGCGCTTGAAACAACTGATAATGCTTTCTATCCCTGGCGCCGAGTGTACGCCATCGCAAACAGTCGCTGCACTGGa                                                                    <  4:218607/149‑1 (MQ=255)
                                                                             aGGCGACTGGTCATGGTGGGCCTCGTCAGGTAGACGTTTGTGACTGCGCAAACGCTACAGCGTTCGCGCAGGCGCTTGAAACAAATGATAATGCTTTCTATCCCTGGCGCCGAGTGTACGCCATCGCAAACAGTCGCTGCACTGGATc                                                                  >  1:162992/1‑148 (MQ=255)
                                                                             aGGCGACTGGTCATGGTGGGCCTCGTCAGGTAGACGTTTGTGACTGCGCAAACGCTACAGCGTTCGCGCAGGCGCTTGAAACAAATGATAATGCTTTCTATCCCTGGCGCCGAGTGTACGCCATCGCAAACAGTCGCTGCACTGGATc                                                                  >  1:157734/1‑148 (MQ=255)
                                                                               gCGACTGGTCATGGTGGGCCTCGTCAGGTAGACGTTTGTGACTGCGCAAACGCTACAGCGTTCGCGCAGGCGCTTGAAACAAATGATAATGCTTTCTATCCCTGGCGCCGAGTGTACGCCATCGCAAACAGTCGCTGCACTGGATCaaa                                                               <  3:146827/149‑1 (MQ=255)
                                                                                      gTCATGGTGGGCCTCGTCAGGTAGACGTTTGTGACTGCGCAAACGCTACAGCGTTCGCGCAGGCGCTTGAAACAAATGATAATGCTTTCTATCCCTGGCGCCGAGTGTACGCCATCGCAAACAGTCGCTGCACTGGATCAAAGACTGa                                                         <  2:200187/148‑1 (MQ=255)
                                                                                      gTCATGGTGGGCCTCGTCAGGTAGACGTTTGTGACTGCGCAAACGCTACAGCGTTCGCGCAGGCGCTTGAAACAAATGATAATGCTTTCTATCCCTGGCGCCGAGTGTACGCCATCGCAAACAGTCGCTGCACTGGATCAAAGACTGAt                                                        >  8:28164/1‑149 (MQ=255)
                                                                                        cATGGTGGGCCTCGTCAGGTAGACGTTTGTGACTGCGCAAACGCTACAGCGTTCGCGCAGGCGCTTGAAACAAATGATAATGCTTTCTATCCCTGGCGCCGAGTGTACGCCATCGCaaa                                                                                    <  6:133915/119‑1 (MQ=255)
                                                                                        cATGGTGGGCCTCGTCAGGTAGACGTTTGTGACTGCGCAAACGCTACAGCGTTCGCGCAGGCGCTTGAAACAAATGATAATGCTTTCTATCCCTGGCGCCGAGTGTACGCCATCGCaaa                                                                                    >  5:133915/1‑119 (MQ=255)
                                                                                           ggtggGCCTCGTCAGGTAGACGTTTGTGACTGCGCAAACGCTACAGCGTTCGCGCAGGCGCTTGAAACAAATGATAATGCTTTCTATCCCTGGCGCCGAGTGTACGCCATCGCAAACAGTCGCTGCACTGGATCAAAGACTGAt                                                        <  7:216648/144‑1 (MQ=255)
                                                                                           ggtggGCCTCGTCAGGTAGACGTTTGTGACTGCGCAAACGCTACAGCGTTCGCGCAGGCGCTTGAAACAAATGATAATGCTTTCTATCCCTGGCGCCGAGTGTACGCCATCGCAAACAGTCGCTGCACTGGATCAAAGACTGAt                                                        >  8:216648/1‑144 (MQ=255)
                                                                                                gCCTCGTCAGGTAGACGTTTGTGACTGCGCAAACGCTACAGCGTTCGCGCAGGCGCTTGAAACAAATGATAATGCTTTCTATCCCTGGCGCCGAGTGTACGCCATCGCAAACAGTCGCTGCACTGGATCAAAGACTGAt                                                        >  7:156632/1‑139 (MQ=255)
                                                                                                gCCTCGTCAGGTAGACGTTTGTGACTGCGCAAACGCTACAGCGTTCGCGCAGGCGCTTGAAACAAATGATAATGCTTTCTATCCCTGGCGCCGAGTGTACGCCATCGCAAACAGTCGCTGCACTGGATCAAAGACTGAt                                                        <  8:156632/139‑1 (MQ=255)
                                                                                                 ccTCGTCAGGTAGACGTTTGTGACTGCGCAAACGCTACAGCGTTCGCGCAGGCGCTTGAAACAAATGATAATGCTTTCTATCCCTGGCGCCGAGTGTACGCCATCGCAAACAGTCGCTGCACTGGATCAAAGACTGATGCCTACGTCg                                              >  7:313107/1‑148 (MQ=255)
                                                                                                 ccTCGTCAGGTAGACGTTTGTGACTGCGCAAACGCTACAGCGTTCGCGCAGGCGCTTGAAACAAATGATAATGCTTTCTATCCCTGGCGCCGAGTGTACGCCATCGCAAACAGTCGCTGCACTGGATCAAAGACTGATGCCTACGTCGa                                             <  8:325033/149‑1 (MQ=255)
                                                                                                      tCAGGTAGACGTTTGTGACTGCGCAAACGCTACAGCGTTCGCGCAGGCGCTTGAAACAAATGATAATGCTTTCTATCCCTGGCGCCGAGTGTACGCGATCGCAAACAGTCGCTGCACTGGATCAAAGCCTGATGCCGACGTGGAAGAc                                         >  8:239891/1‑148 (MQ=255)
                                                                                                              aCGTTTGTGACTGCGCAAACGCTACAGCGTTCGCGCAGGCGCTTGAAACAAATGATAATGCTTTCTATCCCTGGCGCCGAGTGTACGCCATCGCAAACAGTCGCTGCACTGGATCAAAGACTGATGCCTACGTCGAAGACGATGCTGCg                                >  4:211390/1‑149 (MQ=255)
                                                                                                               cGTTTGTGACTGCGCAAACGCTACAGCGTTCGCGCAGGCGCTTGAAACAAATGATAATGCTTTCTATCCCTGGCGCCGAGTGTACGCCATCGCAAACAGTCGCTGCACTGGATCAAAGACTGATGCCTACGTCGAAGACGATGCTGCg                                <  8:313107/148‑1 (MQ=255)
                                                                                                                    gtgACTGCGCAAACGCTACAGCGTTCGCGCATGCGCTTGAAACAAATGATAATGCTTTCTATCCCTGGCGCCGAGTGTACGCCATCGCAAACAGTCGCTGCACTGGATCAAAGACTGATGCCTACGTCGAAGACGATGCTGCGGCCCa                           <  8:334005/148‑1 (MQ=255)
                                                                                                                      gccTGCGCGAACGCTGTAGCGTTTGCGCAGTCGCTTGAAACAAATGATAATGCTTTCTATCCCTGGCGCCGAGTGTACGCCATCGCAAACAGTCGCTGCACTGGATCaaa                                                               >  6:46120/3‑110 (MQ=38)
                                                                                                                      gccTGCGCGAACGCTGTAGCGTTTGCGCAGTCGCTTGAAACAAATGATAATGCTTTCTATCCCTGGCGCCGAGTGTACGCCATCGCAAACAGTCGCTGCACTGGATCaaa                                                               <  5:46120/108‑1 (MQ=38)
                                                                                                                          gcgcAAACGCTACAGCGTTCGCGCAGGCGCTTGAAACAAATGATAATGCTTTCTATCCCTGGCGCCGAGTGTACGCCATCGCAAACAGTCGCTGCTCTGGATCAAAGACTGATGCCTACGTCGAAGACGATGCTGCGGCCCTGGTTGcc                    >  8:105766/1‑149 (MQ=255)
                                                                                                                           cgcgAACGCTGTAGCGTTTGCGCAGTCGCTTGAAACAAATGATAATGCTTTCTATCCCTGGCGCCGAGTGTACGCCATCGCAAACAGTCGCTGCACTGGATCAAAGACTGATGCCTACGTCGAAGACGATGCTGCGGCCCAGGTTGCcg                   >  7:170245/1‑149 (MQ=255)
                                                                                                                               aaCGCTACAGCGTTCGCGCAGGCGCTTGAAACAAATGATAATGCTTTCTATCCCTGGCGCCGAGTGTACGCCATCGCAAACAGTCGCTGCACTGGATCAAAGACTGATGCCTACGTCGAAGACGATGCTGCGGCCCAGGTTGCCGCGCa               <  5:187859/149‑1 (MQ=255)
                                                                                                                                    tACAGCGTTCGCGCAGGCGCTTGAAACAAATGATAATGCTTTCTATCCCTGGCGCCGAGTGTACGCCATCGCAAACAGTCGCTGCACTGGATCAAAGACTGATGCCTACGTCGaa                                            >  6:201696/1‑115 (MQ=255)
                                                                                                                                    tACAGCGTTCGCGCAGGCGCTTGAAACAAATGATAATGCTTTCTATCCCTGGCGCCGAGTGTACGCCATCGCAAACAGTCGCTGCACTGGATCAAAGACTGATGCCTACGTCGaa                                            <  5:201696/115‑1 (MQ=255)
                                                                                                                                      cAGCGTTCGCGCAGGCGCTTGAAACAAATGATAATGCTTTCTATCCCTGGCGCCGAGTGTACGCCATCGCAAACAGTCGCTGCACTGGATCAAAGACTGATGCCTACGTCGAAGACGATGCTGCGGCCCAGGTTGCCGCGCAGGAAATc        >  5:167627/1‑149 (MQ=255)
                                                                                                                                      cAGCGTTCGCGCAGGCGCTTGAAACAAATGATAATGCTTTCTATCCCTGGCGCCGAGTGTACGCCATCGCAAACAGTCGCTGCACTGGATCAAAGACTGATGCCTACGTCGAAGACGATGCTGCGGCCCAGGTTGCCGCGCAGGAAATc        >  1:301228/1‑149 (MQ=255)
                                                                                                                                         cGTTCGCGCAGGCGCTTGAAACAAATGATAATGCTTTCTATCCCTGGCGCCGAGTGTACGCCATCGCAAACAGTCGCTGCACTGGATCAAAGACTGATGCCTACGTCGAAGACGATGCTGCGGCCCAGGTTGCCGCGCAGGAAATCCgg     <  7:28164/149‑1 (MQ=255)
                                                                                                                                         cGTTCGCGCAGGCGCTTGAAACAAATGATAATGCTTTCTATCCCTGGCGCCGAGTGTACGCCATCGCAAACAGTCGCTGCACTGGATCAAAGACTGATGCCTACGTCGAAGACGATGCTGCGGCCCAGGTTGCCGCGCAGGAAATCCgg     <  6:167627/149‑1 (MQ=255)
                                                                                                                                             tgcgcAGTCGCTTGAAACAAATGATAATGCTTTCTATCCCTGGCGCCGAGTGTACGCCATCGCAAACAGTCGCTGCACTGGATCAAAGACTGATGCCTACGTCGAAGACGATGCTGCGGCCCAGGTTGCCGCGCAGGAAATCCGgcgc  <  8:170245/147‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                            
GCCGGAAGGGCCTGCGCTCGACTTGGTGCTGGGGGGCTCGCAGATACTCATCGACACGACGCTGATCTTCCGGGTTCAGGCGACTGGTCATGGTGGGCCTCGTCAGGTAGACGTTTGTGACTGCGCAAACGCTACAGCGTTCGCGCAGGCGCTTGAAACAAATGATAATGCTTTCTATCCCTGGCGCCGAGTGTACGCCATCGCAAACAGTCGCTGCACTGGATCAAAGACTGATGCCTACGTCGAAGACGATGCTGCGGCCCAGGTTGCCGCGCAGGAAATCCGGCGC  >  NC_002947/735484‑735772

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑