Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 779,917 G→T 9.2% A185D (GCC→GAC) ‡ PP_0670 ← bile acid/Na+ symporter family transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_002947779,9170GT 9.2% 196.8 / 9.5 65A185D (GCC→GAC) ‡PP_0670bile acid/Na+ symporter family transporter
Reads supporting (aligned to +/- strand):  ref base G (33/26);  new base T (4/2);  total (37/28)
Fisher's exact test for biased strand distribution p-value = 6.92e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.85e-01

ACCGGAATCACCGGCAGCAAACGGTCGAAATCACGCAGTACCACGTCAATCTGCGAAGCGATCACCACCATCAGCACCAAGGCCATGGCCGGCACCGGCAACCACGCCCAGGCGTCGTTCCAGGTCGCGACTGCGCGCGAGCGCCGGGCACCGGCGCTGGTGAGCACCGCGAGGGCCATCGGCAACACGATCAACAGCACGAAGGCTTCCAGGAACGGGGTGATGGAGATTGCCACCTGGCTGCTGCCACCCAGCATCAGGGCCAGGTACACCGGCAGCAGCACCAGTTGCGCC  >  NC_002947/779770‑780063
                                                                                                                                                   |                                                                                                                                                  
aCCGGAATCACCGGCAGCAAACGGTCGAAATCACGCAGTACCACGTCAATCTGCGAAGCGATCACCACCATCAGCACCAAGGCCATGGCCGGCACCGGCAACCACGCCCAGGCGTCGTTCCAGGTCGCGACTGCGCGCGAGCGCCggg                                                                                                                                                    >  3:62418/1‑148 (MQ=255)
 ccGGAATCACCGGCAGCAAACGGTCGAAATCACGCAGTACCACGTCAATCTGCGAAGCGATCACCACCATCAGCACCAAGGCCATGGCCGGCACCGGCAACCACGCCCAGGCGTCGTTCCAGGTCGCGACTGCGCGCGAGCGCCGGGCa                                                                                                                                                  >  6:207181/1‑149 (MQ=255)
  cGGAATCACCGGCAGCAAACGGTCGAAATCACGCAGTACCACGTCAATCTGCGAAGCGATCACCACCATCAGCACCAAGGCCATGGCCGGCACCGGCAACCACGCCCAGGCGTCGTTCCAGGTCGCGACTGCGCGCGAGCGCCGGGCAc                                                                                                                                                 >  3:17679/1‑149 (MQ=255)
        cACCGGCAGCAAACGGTCGAAATCACGCAGTACCACGTCAATCTGCGAAGCGATCACCACCATCAGCACCAAGGCCATGGCCGGCACCGGCAACCACGCCCAGGCGTCGTTCCAGGTCGCGACTGCGCGCGAGCGCCGGGCACCGgcgc                                                                                                                                           <  3:79513/149‑1 (MQ=255)
          ccGGCAGCAAACGGGCGAAATCACGCAGTACCACGTCAATCTGCGAAGCGATCACCACCATCAGCACCAAGGCCATGGCCGGCACCGGCAACCACGCCCAGGCGTCGTTCCAGGTCGCGACTGCGCGCGAGCGCCGGGCACCGGCGCTg                                                                                                                                         >  7:299499/1‑149 (MQ=255)
             gcagcaAACGGTCGAAATCACGCAGTACCACGTCAATCTGCGAAGCGATCACCACCATCAGCACCAAGGCCATGGCCGGCACCGGCAACCACGCCCAGGCGTCGTTCCAGGTCGCGACTGCGCGCGAGCGCCGGGCACCGGCGCTGGTg                                                                                                                                      <  4:62418/149‑1 (MQ=255)
               agcaAACGGTCGAAATCACGCAGTACCACGTCAATCTGCGAAGCGATCACCACCATCAGCACCAAGGCCATGGCCGGCACCGGCAACCACGCCCAGGCGTCGTTCCAGGTCGCGACTGCGCGCGAGCGCCGGGCACCGGCGCTGGTGAg                                                                                                                                    <  8:299499/149‑1 (MQ=255)
                      ggTCGAAATCACGCAGTACCACGTCAATCTGCGAAGCGATCACCACCATCAGCACCAAGGCCATGGCCGGCACCGGCAACCACGCCCAGGCGTCGTTCCAGGTCGCGACTGCGCGCCAGCGCCGGTGCCCGGCGCtcgc                                                                                                                                       <  3:203160/139‑4 (MQ=255)
                      ggTCGAAATCACGCAGTACCACGTCAATCTGCGAAGCGATCACCACCATCAGCACCAAGGCCATGGCCGGCACCGGCAACCACGCCCAGGCGTCGTTCCAGGTCGCGACTGCGCGCCAGCGCCGGTGCCCGGCGCtcgc                                                                                                                                       >  4:203160/1‑136 (MQ=255)
                       gTCGAAATCACGCAGTACCACGTCAATCTGCGAAGCGATCACCACCTTCAGCACCAAGGCCATGGCCGGCACCGGCAACCACGCCCAGGCGTCGTTCCAGGTCGCGACTGCGCGCGAGCGCCGGGCACCGgcg                                                                                                                                            >  6:184609/1‑133 (MQ=255)
                        tCGAAATCACGCAGTACCACGTCAATCTGCGAAGCGATCACCACCATCAGCACCAAGGCCATGGCCGGCACCGGCAACCACGCCCAGGCGTCGTTCCAGGTCGCGACTGCGCGCGAGCGCCGGGCACCGGCGCTGGTGAGCACCGCGAg                                                                                                                           >  1:327196/1‑149 (MQ=255)
                              tCACGCAGTACCACGTCAATCTGCGAAGCGATCACCACCATCAGCACCAAGGCCATGGCCGGCACCGGCAACCACGCCCAGGCGTCGTTCCAGGTCGCGACTGCGCGCGAGCGCCGGGCACCGGCGCTGGTGAGCACCGCGAGGGCCAt                                                                                                                     >  4:306335/1‑149 (MQ=255)
                                         cACGTCAATCTGCGAAGCGATCACCACCATCAGCACCAAGGCCATGGCCGGCACCGGCAACCACGCCCAGGCGTCGTTCCAGGTCGCGACTGCGCGCGAGCGCCGGGCACCGGCGCTGGTGAGCACCGCGAGGGCCATCGGCAACACGa                                                                                                          >  3:155334/1‑149 (MQ=255)
                                         cACGTCAATCTGCGAAGCGATCACCACCATCAGCACCAAGGCCATGGCCGGCACCGGCAACCACGCCCAGGCGTCGTTCCAGGTCGCGACTGCGCGCGAGCGCCGGGCACCGGCGCTGGTGAGCACCGCGAGGGCCATCGGCAACACGa                                                                                                          >  1:168647/1‑149 (MQ=255)
                                          aCGTCAATCTGCGAAGCGATCACCACCATCAGCACCAAGGCCATGGCCGGCACCGGCAACCACGCCCAGGCGTCGTTCCAGGTCGCGACTGCGCGCGAGCGCCGGGCACCGGCGCTGGTGAGCACCGCGAGGGCCATCGGCAACACGAt                                                                                                         <  5:207181/149‑1 (MQ=255)
                                             tCAATCTGCGAAGCGATCACCACCATCAGCACCAAGGCCATGGCCGGCACCGGCAACCACGCCCAGGCGTCGTTCCAGGTCGCGACTGCGCGCGAGCGCCGGGCACCGGCGCTGGTGAGCACCGCGAGGGCCATCGGCAACACGATCaa                                                                                                      <  4:17679/149‑1 (MQ=255)
                                               aaTCTGCGAAGCGATCACCACCATCAGCACCAAGGCCATGGCTGGCACCGGCAACCACGCCCAGGCGTCGTTCCAGGTCGCGACTGCGCGCGAGCGCCGGGCACCGGCGCTGGTGAGCACCGCGAGGGCCATCGGCAACACGATCAACa                                                                                                    <  8:31942/149‑1 (MQ=255)
                                                   tGCGAAGCGATCACCACCATCAGCACCAAGGCCATGGCCGGCACCGGCAACCACGCCCAGGCGTCGTTCCAGGTCGCGACTGCGCGCGAGCGCCGGGCACCGGCGCTGGTGAGCACCGCGAGGGCCATCGGCAACACGATCAACAGCAc                                                                                                >  1:296468/1‑149 (MQ=255)
                                                   tGCGAAGCGATCACCACCATCAGCACCAAGGCCATGGCCGGCACCGGCAACCACGCCCAGGCGTCGTTCCAGGTCGCGACTGCGCGCGAGCGCCGGGCACCGGCGCTGGTGAGCACCGCGAGGGCCATCGGCAACACGATCAACAGCAc                                                                                                >  1:301335/1‑149 (MQ=255)
                                                    gCGAAGCGATCACCACCATCAGCACCAAGGCCATGGCCGGCACCGGCAACCACGCCCAGGCGTCGTTCCAGGTCGCGACTGCGCGCGAGCGCCGGGCACCGGCGCTGGTGAGCACCGCGAgg                                                                                                                          >  3:262351/1‑122 (MQ=255)
                                                    gCGAAGCGATCACCACCATCAGCACCAAGGCCATGGCCGGCACCGGCAACCACGCCCAGGCGTCGTTCCAGGTCGCGACTGCGCGCGAGCGCCGGGCACCGGCGCTGGTGAGCACCGCGAgg                                                                                                                          <  4:262351/122‑1 (MQ=255)
                                                     cGAAGCGATCACCACCATCAGCACCAAGGCCATGGCCGGCACCGGCAACCACGCCCAGGCGTCGTTCCAGGTCGCGACTGCGCGCGAGCGCCGGGCACCGGCGCTGGTGAGCACCGCGAGGGCCATCGGCAACACGATCAACAGc                                                                                                  <  3:306335/145‑1 (MQ=255)
                                                     cGAAGCGATCACCACCATCAGCACCAAGGCCATGGCCGGCACCGGCAACCACGCCCAGGCGTCGTTCCAGGTCGCGACTGCGCGCGAGCGCCGGGCACCGGCGCTGGTGAGCACCGCGAGGGCCATCGGCAACACGATCAACAGCACGa                                                                                              <  6:346959/149‑1 (MQ=255)
                                                            aTCACCACCATCAGCACCAAGGCCATGGCCGGCACCGGCAACCACGCCCAGGCGTCGTTCCAGGTCGCGACTGCGCGCGAGCGCCGGGCACCGGCGCTGGTGAGCACCGCGAGGGCCATCGGCAACACGATCAACAGCACGAAGGCTTc                                                                                       <  4:155334/149‑1 (MQ=255)
                                                               accaccATCAGCACCAAGGCCATGGCCGGCACCGGCAACCACGCCCAGGCGTCGTTCCAGGTCGCGACTGCGCGCGAGCGCCGGGCACCGGCGCTGGTGAGCACCGCGAGGGCCATCGGCAACACGATCAACAGCACGAAGGCTTCCAg                                                                                    <  6:17288/149‑1 (MQ=255)
                                                                   ccaTCAGCACCAAGGCCATGGCCGGCACCGGCAACCACGCCCAGGCGTCGTTCCAGGTCGCGACTGCGCGCGAGCGCCGGGCACCGGCGCTGGTGAGCACCGCGAGGGCCATCGGCAACACGATCAACAGCACGAAGGCTTCCAGGAAc                                                                                >  4:36771/1‑149 (MQ=255)
                                                                    caTCAGCACCAAGGCCATGACCGGCACCGGCAACCACGCCCAGGCGTCGTTCCAGGTCGCGACTGCGCGCGAGCGCCGGGCACCGGCGCTGGTGAGCACCGCGAGGGCCATCGGCAACACGATCAACAGCACGAAGGCTTCCAGGAACg                                                                               >  6:284654/1‑149 (MQ=255)
                                                                      tCAGCACCAAGGCCATGGCCGGCACCGGCAACCACGCCCAGGCGTCGTTCCAGGTCGCGACTGCGCGCGAGCGCCGGGCACCGGCGCTGGTGAGCACCGCGAGGGCCATCGGCAaca                                                                                                             <  3:175536/117‑1 (MQ=255)
                                                                      tCAGCACCAAGGCCATGGCCGGCACCGGCAACCACGCCCAGGCGTCGTTCCAGGTCGCGACTGCGCGCGAGCGCCGGGCACCGGCGCTGGTGAGCACCGCGAGGGCCATCGGCAaca                                                                                                             >  4:175536/1‑117 (MQ=255)
                                                                      tCAGCACCAAGGCCATGGCCGGCACCGGCAACCACGCCCAGGCGTCGTTCCAGGTCGCGACTGCGCGCGAGCGCCGGGCACCGGCGCTGGTGAGCACCGCGAGGGCCATCGGCAACACGATCAACAGCACGAAGGCTTCCAGGAACggg                                                                             >  1:212312/1‑149 (MQ=255)
                                                                       cAGCACCAAGGCCATGGCCGGCACCGGCAACCACGCCCAGGCGTCGTTCCAGGTCGCGACTGCGCGCGAGCGCCGGGCACCGGCGCTGGTGAGCACCGCGAGGGCCATCGGc                                                                                                                 <  4:180496/112‑1 (MQ=255)
                                                                           aCCAAGGCCATGACCGGCACCGGCAACCACGCCCAGGCGTCGTTCCAGGTCGCGACTGCGCGCGAGCGCCGGGCACCGGCGCTGGTGAGCACCGCGAGGGCCATCGGCAACACGATCAACAGCACGAAGGCTTCCAGGAACGGGGTGAt                                                                        <  5:284654/149‑1 (MQ=255)
                                                                            ccAAGGCCATGGCCGGCACCGGCAACCACGCCCAGGCGTCGTTCCAGGTCGCGACTGCGCGCGAGCGCCGGGCACCGGCGCTGGTGAGCACCGCGAGGGCCATCGGCAACACGATCAACAGCACGAAGGCTTCCAGGAACGGGGTGATg                                                                       <  7:332247/149‑1 (MQ=255)
                                                                            ccAAGGCCATGGCCGGCACCGGCAACCACGCCCAGGCGTCGTTCCAGGTCGCGACTGCGCGCGAGCGCCGGGCACCGGCGCTGGTGAGCACCGCGAGGGCCATCGGCAACACGATCAACAGCACGAAGGCTTCCAGGAACGGGGTGATg                                                                       >  3:330655/1‑149 (MQ=255)
                                                                             cAAGGCCATGGCCGGCACCGGCAACCACGCCCAGGCGTCGTTCCAGGTCGCGACTGCGCGCGAGCGCCGGGCACCGGCGCTGGTGAGCACCGCGAGGGCCATCGGCAACACGATCAACAGCACGAAGGCTTCCAGGAACGGGGTGATgg                                                                      <  3:36771/149‑1 (MQ=255)
                                                                             cAAGGCCATGGCCGGCACCGGCAACCACGCCCAGGCGTCGTTCCAGGTCGCGACTGCGCGCGAGCGCCGGGCACCGGCGCTGGTGAGCACCGCGAGGGCCATCGGCAACACGATCAACAGCACGAAGGCTTCCAGGAACGGGGTGATg                                                                       >  2:53744/1‑148 (MQ=255)
                                                                             cAAGGCCATGGCCGGCACCGGCAACCACGCCCAGGCGTCGTTCCAGGTCGCGACTGCGCGCGAGCGCCGGGCACCGGCGCTGGTGAGCACCGCGAGGGCCATCGGCAACACGATCAACAGCACGAAGGCTTCCAGGAACGGGGTGATg                                                                       >  5:3615/1‑148 (MQ=255)
                                                                              aaGGCCATGGCCGGCACCGGCAACCACGCCCAGGCGTCGTTCCAGGTCGCGACTGCGCGCGAGCGCCGGGCACCGGCGCTGGTGAGCACCGCGAGGGCCATCGGCAACACGATCAACAGCACGAAGGCTTCCAGGAACGGGGTGATGga                                                                     <  6:3615/149‑1 (MQ=255)
                                                                                  ccATGGCCGGCACCGGCAACCACGCCCAGGCGTCGTTCCAGGTCGCGACTGCGCGCGAGCGCCGGGCACCGGCGCTGGTGAGCACCGCGAGGGCCATCGGCAACACGATCAACAGCACGAAGGCTTCCAGGAACGGGGTGATGGAGAtt                                                                 <  2:168647/149‑1 (MQ=255)
                                                                                         cggcaccggcaACCACGCCCAGGCGTCGTTCCAGGTCGCGACTGCGCGCGAGCGCCGGGCACCGGCGCTGGTGAGCACCGCGAGGGCCATCGGCAACACGATCAACAGCACGAAGGCTTCCAGGAACGGGGTGATGgag                                                                    >  6:358147/1‑139 (MQ=255)
                                                                                         cggcaccggcaACCACGCCCAGGCGTCGTTCCAGGTCGCGACTGCGCGCGAGCGCCGGGCACCGGCGCTGGTGAGCACCGCGAGGGCCATCGGCAACACGATCAACAGCACGAAGGCTTCCAGGAACGGGGTGATGgag                                                                    <  5:358147/139‑1 (MQ=255)
                                                                                             accggcaACCACGCCCAGGCGTCGTTCCAGGTCGCGACTGCGCGCGAGCGCCGGGCACCGGCGCTGGTGAGCACCGCGAGGGCCATCGGCAACACGATCAACAGCACGAAGGCTTCCAGGAACGGGGTGATGGAGATTGCCACCTGgct                                                      >  1:303401/1‑149 (MQ=255)
                                                                                               cggcaACCACGCCCAGGCGTCGTTCCAGGTCGCGACTGCGCGCGAGCGCCGGGCACCGGCGCTGGTGAGCACCGCGAGGGCCATCGGCAACACGATCAACAGCACGAAGGCTTCCAGGAACGGGGTGATGGAGATTGCCACCTGgctgc                                                    >  5:149617/1‑149 (MQ=255)
                                                                                                 gcaACCACGCCCAGGCGTCGTTCCAGGTCGCGACTGCGCGCGAGCGCCGGGCACCGGCGCTGGTGAGCACCGCGAGGGCCATCGGCAACACGATCAACAGCACGAAGGCTTCCAGGAACggg                                                                             <  6:274744/122‑1 (MQ=255)
                                                                                                 gcaACCACGCCCAGGCGTCGTTCCAGGTCGCGACTGCGCGCGAGCGCCGGGCACCGGCGCTGGTGAGCACCGCGAGGGCCATCGGCAACACGATCAACAGCACGAAGGCTTCCAGGAACggg                                                                             >  5:274744/1‑122 (MQ=255)
                                                                                                 gcaACCACGCCCAGGCGTCGTTCCAGGTCGCGACTGCGCGCGAGCGCCGGGCACCGGCGCTGGTGAGCACCGCGAGGGCCATCGGCAACACGATCAACAGCACGAAGGCTTCCAGGAACGGGGTGATGGAGATTGCCACCTGGctgctg                                                  >  6:82240/1‑149 (MQ=255)
                                                                                                    aCCACGCCCAGGCGTCGTTCCAGGTCGCGACTGCGCGCGAGCGCCGGGCACCGGCGCTGGTGAGCACCGCGAGGGCCATCGGCAACACGATCAACAGCACGAAGGCTTCCAGGAACggg                                                                             <  5:28566/119‑1 (MQ=255)
                                                                                                    aCCACGCCCAGGCGTCGTTCCAGGTCGCGACTGCGCGCGAGCGCCGGGCACCGGCGCTGGTGAGCACCGCGAGGGCCATCGGCAACACGATCAACAGCACGAAGGCTTCCAGGAACggg                                                                             >  6:28566/1‑119 (MQ=255)
                                                                                                    aCCACGCCCAGGCGTCGTTCCAGGTCGCGACTGCGCGCGAGCGCCGGGCACCGGCGCTGGTGAGCACCGCGAGGGCCATCGGCAACACGATCAACAGCACGAAGGCTTCCAGGAACGGGGTGATGGAGATTGCCACCTGGCTGCTGCCa                                               >  3:82490/1‑149 (MQ=255)
                                                                                                      cACGCCCAGGCGTCGTTCCAGGTCGCGACTGCGCGCGAGCGCCGGGCACCGGCGCTGGTGAGCACCGCGAGGGCCATCGGCAACACGATCAACAGCACGAAGGCTTCCAGGAACGGGGTGa                                                                         >  6:215888/1‑121 (MQ=255)
                                                                                                      cACGCCCAGGCGTCGTTCCAGGTCGCGACTGCGCGCGAGCGCCGGGCACCGGCGCTGGTGAGCACCGCGAGGGCCATCGGCAACACGATCAACAGCACGAAGGCTTCCAGGAACGGGGTGATGGAGATTGCCACCTGGCTGCTGCCAcc                                             <  6:149617/149‑1 (MQ=255)
                                                                                                                cgtcgtTCCAGGTCGCGACTGCGCGCGAGCGCCGGGCACCGGCGCTGGTGAGCACCGCGAGGGCCATCGGCAACACGATCAACAGCACGAAGGCTTCCAGGAACGGGGTGATGGAGATTGCCACCTGGCTGCTGCCACCCAGCATCAgg                                   <  2:327196/149‑1 (MQ=255)
                                                                                                                     ttCCAGGTCGCGACTGCGCGCGAGCGCCGGGCACCGGCGCTGGTGAGCACCGCGAGGGCCATCGGCAACACGATCAACAGCACGAAGGCTTCCAGGAACGGGGTGATGGAGATTGCCACCTGGCTGCTGCCACCCAGCATCAGGGCCAg                              >  7:206483/1‑149 (MQ=255)
                                                                                                                      tCCAGGTCGCGACTGCGCGCGAGCGCCGGGCACCGGCGCTGGTGAGCACCGCGAGGGCCATCGGCAACACGATCAACAGCACGAAGGCTTCCAGGAACGGGGTGATGGAGATTGCCACCTGGCTGCTGCCACCCAGCATCAGGGCCAgg                             >  3:18735/1‑149 (MQ=255)
                                                                                                                                   tGCGCGCGAGCGCCGGGCACCGGCGCTGGTGAGCACCGCGAGGGCCATCGGCAACACGATCAACAGCACGAAGGCTTCCAGGAACGGGGTGATGGAGATTGCCACCTGGCTGCTGCCACCCAGCATCAgg                                   >  4:74211/1‑130 (MQ=255)
                                                                                                                                      gcgcgAGCGCCGGGCACCGGCGCTGGTGAGCACCGCGAGGGCCATCGGCAACACGATCAACAGCACGAAGGCTTCCAGGAACGGGGTGATGGAGATTGCCACCTGGCTGCTGCCACCCAGCATCAGGGCCAGGTACACCGgcagcagca             >  6:341867/1‑149 (MQ=255)
                                                                                                                                       cgcgAGCGCCGGGCACCGGCGCTGGTGAGCACCGCGAGGGCCATCGGCAACACTATCAACAGCACGAAGGCTTCCAGGAACGGGGTGATGGAGATTGCCACCTGGCTGCTGCCACCCAGCATCAGGGCCAGGTACACCGGCAGCAGCAc            <  4:18735/149‑1 (MQ=255)
                                                                                                                                        gcgAGCGCCGGGCACCGGCGCTGGTGAGCACCGCGAGGGCCATCGGCAACACGATCAACAGCACGAAGGCTTCCAGGAACGGGGTGATGGAGATTGCCACCTGGCTGCTGCCACCCAGCATCAGGGCCAGGTACACCgg                     <  5:36338/139‑1 (MQ=255)
                                                                                                                                        gcgAGCGCCGGGCACCGGCGCTGGTGAGCACCGCGAGGGCCATCGGCAACACGATCAACAGCACGAAGGCTTCCAGGAACGGGGTGATGGAGATTGCCACCTGGCTGCTGCCACCCAGCATCAGGGCCAGGTACACCgg                     >  6:36338/1‑139 (MQ=255)
                                                                                                                                         ccaGCGCCGGTGCCCGGCGCTGGTGAGCACCGCGAGGGCCATCGGCAACACGATCAACAGCACGAAGGCTTCCAGGAACGGGGTGATGGAGATTGCCACCTGGCTGCTGCCACCCAg                                          >  2:22401/3‑117 (MQ=255)
                                                                                                                                         ccaGCGCCGGTGCCCGGCGCTGGTGAGCACCGCGAGGGCCATCGGCAACACGATCAACAGCACGAAGGCTTCCAGGAACGGGGTGATGGAGATTGCCACCTGGCTGCTGCCACCCAg                                          <  1:22401/115‑1 (MQ=255)
                                                                                                                                          caGCGCCGGTGCCCGGCGCTGGTGAGCACCGCGAGGGCCATCGGCAACACGATCAACAGCACGAAGGCTTCCAGGAACGGGGTGATGGAGATTGCCACCTGGCTGCTGCCACCCAGCATCAGGGCCAGGTACACCGGCAGCAGCACCAg         >  3:26444/2‑149 (MQ=255)
                                                                                                                                          caGCGCCGGTGCCCGGCGCTGGTGAGCACCGCGAGGGCCATCGGCAACACGATCAACAGCACGAAGGCTTCCAGGAACGGGGTGATGGAGATTGCCACCTGGCTGCTGCCACCCAGCATCAGGGCCAGGTACACCGGCAGCAGCACCAg         >  7:43522/2‑149 (MQ=255)
                                                                                                                                              gcCGGGCACCGGCGCTGGTGAGCACCGCGAGGGCCATCGGCAACACGATCAACAGCACGAAGGCTTCCAGGAACGGGGTGATGGAGATTGCCACCTGGCTGCTGCCACCCAGCATCAGGGCCAGGTACACCGGCAGCAGCACCAGTTgc     <  5:82240/149‑1 (MQ=255)
                                                                                                                                              gcCGGGCACCGGCGCTGGTGAGCACCGCGAGGGCCATCGGCAACACGATCAACAGCACGAAGGCTTCCAGGAACGGGGTGATGGAGATTGCCACCTGGCTGCTGCCACCCAGCATCAGGGCCAGGTACACCGGCAGCAGCACCAGTTgc     >  2:127206/1‑149 (MQ=255)
                                                                                                                                               ccGGGCACCGGCGCTGGTGAGCACCGCGAGGGCCATCGGCAACACGATCAACAGCACGAAGGCTTCCAGGAACGGGGTGATGGAGATTGCCACCTGGCTGCTGCCACCCAGCATCAGGGCCAGGTACACCGGCAGCAGCACCAGTTgcg    >  7:124688/1‑149 (MQ=255)
                                                                                                                                                 gggCACCGGCGCTGGTGAGCACCGCGAGGGCCATCGGCAACACGATCAACAGCACGAAGGCTTCCAGGAACGGGGTGATGGAGATTGCCACCTGGCTGCTGCCACCCAGCATCAGGGCCAGGTACACCGGCAGCAGCACCAGTTGCGcc  <  1:53744/149‑1 (MQ=255)
                                                                                                                                                  ggCACCGGCGCTGGTGAGCACCGCGAGGGCCATCGGCAACACGATCAACAGCACGAAGGCTTCCAGGAACGGGGTGATGGAGATTGCCACCTGGCTGCTGCCACCCAGCATCAGGGCCAGGTACACCGGCAGCAGCACCAGTTgcgc   >  6:133514/1‑147 (MQ=255)
                                                                                                                                                  ggCACCGGCGCTGGTGAGCACCGCGAGGGCCATCGGCAACACGATCAACAGCACGAAGGCTTCCAGGAACGGGGTGATGGAGATTGCCACCTGGCTGCTGCCACCCAGCATCAGGGCCAGGTACACCGGCAGCAGCACCAGTTgcgc   >  4:249929/1‑147 (MQ=255)
                                                                                                                                                   |                                                                                                                                                  
ACCGGAATCACCGGCAGCAAACGGTCGAAATCACGCAGTACCACGTCAATCTGCGAAGCGATCACCACCATCAGCACCAAGGCCATGGCCGGCACCGGCAACCACGCCCAGGCGTCGTTCCAGGTCGCGACTGCGCGCGAGCGCCGGGCACCGGCGCTGGTGAGCACCGCGAGGGCCATCGGCAACACGATCAACAGCACGAAGGCTTCCAGGAACGGGGTGATGGAGATTGCCACCTGGCTGCTGCCACCCAGCATCAGGGCCAGGTACACCGGCAGCAGCACCAGTTGCGCC  >  NC_002947/779770‑780063

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑