Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	780,558	T→A	11.6%	intergenic (‑88/+34)	PP_0670 ← / ← glyA‑II	bile acid/Na+ symporter family transporter/serine hydroxymethyltransferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	780,558	0	T	A	11.6%	108.3 / 6.5	43	intergenic (‑88/+34)	PP_0670/glyA‑II	bile acid/Na+ symporter family transporter/serine hydroxymethyltransferase
Reads supporting (aligned to +/- strand):  ref base T (20/18);  new base A (3/2);  total (23/20)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.76e-01

CGGCCAGTACGGCGGCGAAGTAGATGGGGATCTGCCGGGTTTCGAGTTGCTCTCTGGTCAAGGTGCGGCCTGTGCAAAGTGGAGGTATTGGGGGCGCAGTCTTTGTACGAGGGGCGGTGCGCCTGTATTAAACGAAAGAGCCCGGCACTGGGCCGGGCTCTTTCACTTTAGGCAAGCGCCAATCAGTTGCCGTAAACCGGCAGCTTCTTGCAGATGGCCTTGACCTTCTCACGGACGGCGTCGATCACCGCTTCGTTGTTCAGGTCGGCCAGGATGTCGCAGATCCAGCCGGC  >  NC_002947/780410‑780702                                                                                                                                                     |                                                                                                                                                 cGGCCAGTACGGCGGCGAAGTAGATGGGGATCTGCCGGGTTTCGAGTTGCTCTCTGGTCAAGGTGCGGCCTGTGCAAAGTGGAGGTATTGGGGGCGCAGTCTTTGTACGAGGGGCGGTGCGCCTGTATTAAACGAAAGAGCCCGGCACt                                                                                                                                                  >  8:40355/1‑149 (MQ=255)   gCCAGTACGGCGGCGAAGTAGATGGGGATCTGCCGGGTTTCGAGTTGCTCTCTGGTCAAGGTGCGGCCTGTGCAAAGTGGAGGTATTGGGGGCGCAGTCTTTGTACGAGGGGCGGTGCGCCTGTATTAAACGAAAGAGCCCGGCACTgg                                                                                                                                                >  5:317122/1‑149 (MQ=255)   gCCAGTACGGCGGCGAAGTAGATGGGGATCTGCCGGGTTTCGAGTTGCTCTCTGGTCAAGGTGCGGCCTGTGCAAAGTGGAGGTATTGGGGGCGCAGTCTTTGTACGAGGGGCGGTGCGCCTGTATTAAACGAAAGAGCCCGGCACTgg                                                                                                                                                >  5:310240/1‑149 (MQ=255)       gTACGGCGGCGAAGTAGATGGGGATCTGCCGGGTTTCGAGTTGCTCTCTGGTCAAGGTGCGGCCTGTGCAAAGTGGAGGTATTGGGGGCGCAGTCTTTGTACGAGGGGCGGTGCGCCTGTATTAAACGAAAGAGCCCGGCACTGGGCCg                                                                                                                                            <  6:317122/149‑1 (MQ=255)               gcgAAGTAGATGGGGATCTGCCGGGTTTCGAGTTGCTCTCTGGTCAAGGTGCGGCCTGTGCAAAGTGGAGGTATTGGGGGCGCAGTCTTTGTACGAGGGGCGGTGCGCCTGTATTAAACGAAAGAGCCCGGCACTGGGCCGGGCTCttt                                                                                                                                    <  6:175771/149‑1 (MQ=255)                       gATGGTGATCTGCTGGGTTTCGATTTTCGATCTGGTCAAGGTGCGTCCTGTGCATTTTGTAGGTTTTGGGGGCGCTGTCTTTGTACGAGGGGCGGTGCGCCTGTATTAATCGAAAGAGCCCGGCACTGGGCCGGTCTCTTTCACTTTa                                                                                                                             <  6:310240/148‑1 (MQ=255)                       gATGGGGATCTGCCGGGGTTCGAGTTGCTCTCTGGACAAGGTGCGGCCTGTGCAAAGTGGAGGTATTGGGGGCGCAGTCTTTGTACGAGGGGCGGTGCGCCTGTATTAAACGAAAGAGCCCGGCACTGGGCCGGGCTCTTTCACTTTa                                                                                                                             <  7:327299/148‑1 (MQ=255)                            ggATCTGCCGGGTTTCGAGTTGCTCTCTGGTCAAGGTGCGGCCTGTGCAAAGTGGAGGTATTGGGGGCGCAGTCTTTGTACGAGGGGCGGTGCGCCTGTATTAAACGAAAGAGCCCGGCACTGGGCCGGGCTCTTTCACTTTAGGCAAg                                                                                                                       >  2:205181/1‑149 (MQ=255)                                   ccGGGTTTCGAGTTGCTCTCTGGTCAAGGTGCGGCCTGTGCAAAGTGGAGGTATTGGGGGCGCAGTCTTTGTACGAGGGGCGGTGCGCCTGTATTAAACGAAAGAGCCCGGCACTGGGCCGGGCTCTTTCACTTTAGGCAAGCGCCaa                                                                                                                 >  3:155443/1‑148 (MQ=255)                                     gggTTTCGAGTTGCTCTCTGGTCAAGGTGCGGCCTGAGCAAAGTGGAGGTATTGGGGGCGCAGTCTTTGTACGAGGGGCGGTGCGCCTGTATTAAACGAAAGAGCCCGGCACTGGGCCGGGCTCTTTCACTTTAGGCAAGCGCCAAt                                                                                                                >  4:26850/1‑147 (MQ=255)                                              gTTGCTCTCTGGTCAAGGTGCGGCCTGTGCAAAGTGGAGGTATTGGGGGCGCAGTCTTTGTACGAGGGGCGGTGCGCCTGTATTAAACGAAAGAGCCCGGCACTGGGCCGGGCTCTTTCACTTTAGGCAAgcgc                                                                                                                    <  8:290800/134‑1 (MQ=255)                                              gTTGCTCTCTGGTCAAGGTGCGGCCTGTGCAAAGTGGAGGTATTGGGGGCGCAGTCTTTGTACGAGGGGCGGTGCGCCTGTATTAAACGAAAGAGCCCGGCACTGGGCCGGGCTCTTTCACTTTAGGCAAgcgc                                                                                                                    >  7:290800/1‑134 (MQ=255)                                                  ctctctGGTCAAGGTGCGGCCTGTGCAAAGTGGAGGTATTGGGGGCGCAGTCTTTGTACGAGGGGCGGTGCGCCTGTATTAAACGAAAGAGCCCGGCACTGGGCCGGGCTCTTTCACTTTAGGCAAGCGCCAATCAGTTGCCGTAAAcc                                                                                                 <  1:205181/149‑1 (MQ=255)                                                   tctctGGTCAAGGTGCGGCCTGTGCAAAGTGGAGGTATTGGGGGCGCAGTCTTTGTACGAGGGGCGGTGCGCCTGTATTAAACGAAAGAGCCCGGCACTGGGCCGGGCTCttt                                                                                                                                    <  8:93991/113‑1 (MQ=255)                                                   tctctGGTCAAGGTGCGGCCTGTGCAAAGTGGAGGTATTGGGGGCGCAGTCTTTGTACGAGGGGCGGTGCGCCTGTATTAAACGAAAGAGCCCGGCACTGGGCCGGGCTCttt                                                                                                                                    >  7:93991/1‑113 (MQ=255)                                                         gTCAAGGTGCGGCCTGTGCAAAGTGGAGGTATTGGGGGCGCAGTCTTTGTACGAGGGGCGGTGCGCCTGTATTAAACGAAAGAGCCCGGCCCAGTGCCGGGct                                                                                                                                        <  8:208086/103‑1 (MQ=255)                                                         gTCAAGGTGCGGCCTGTGCAAAGTGGAGGTATTGGGGGCGCAGTCTTTGTACGAGGGGCGGTGCGCCTGTATTAAACGAAAGAGCCCGGCCCAGTGCCGGGct                                                                                                                                        >  7:208086/1‑103 (MQ=255)                                                             aGGTGCGGCCTGTGCAAAGTGGAGGTATTGGGGGCGCAGTCTTTGTACGAGGGGCGGTGCGCCTGTATTAAACGAAAGAGCCCGGCACTGGGCCGGGCTCTTTCACTTTAGGCAAGCGCCAATCAGTTGCCGTAAACCGGCAGcttctt                                                                                      <  3:286813/149‑1 (MQ=255)                                                                    gCCTGTGCAAAGTGGAGGTATTGGGGGCGCAGTCTTTGTACGAGGGGCGGTGCGCCTGTATTAAACGAAAGAGCCCGGCACTGGGCCGGGCTCTTTCACTTTAGGCAAGCGCCAATCAGTTGCCGTAAACCGGCAGCTTCTTGCAGATg                                                                               >  3:174140/1‑149 (MQ=255)                                                                         tgCAAAGTGGAGGTATTGGGGGCGCAGTCTTTGTACGAGGGGCGGTGCGCCTGTATTAAACGAAAGAGCCCGGCACTGGGCCGGGCTCTTTCACTTTAGGCAAGCGCCAATCAGTTGCCGTAAACCGGCAGCTTCTTGCAGATGGCCtt                                                                          <  4:174140/149‑1 (MQ=255)                                                                           cAAAGTGGAGGTATTGGGGGCGCAGTCTTTGTACGAGGGGCGGTGCGCCTGTATTAAACGAAAGAGCCCGGCACTGGGCCGGGctct                                                                                                                                      >  3:17168/1‑87 (MQ=255)                                                                                  gAGGTATTGGGGGCGCAGTCTTTGTACGAGGGGCGGTGCGCCTGTATTAAACGAAAGAGCCCGGCACTGGGCCGGGCTCTTTCACTTTAGGCAAGCGCCAATCAGTTGCCGTAAACCGGCAGCTTCTTGCAGATGGCCTTGACCTtct                                                                  >  7:175608/1‑148 (MQ=255)                                                                                         tGGGGGCGCAGTCTTTGTACGAGGGGCGGTGCGCCTGTATTAAACGAAAGAGCCCGGCACTGGGCCGGGCTCTTTCACTTTAGGCAAGCGCCAATCAGTTGCCGTAAACCGGCAGCTTCTTGCAGATGGCCTTGACCTTCTCacggac                                                           <  8:175608/148‑1 (MQ=255)                                                                                          gggggCGCAGTCTTTGTACGAGGGGCGGTGCGCCTGTATTAAACGAAAGAGCCCGGCACTGGGCCGGGCTCTTTCACTTTAGGCAAGCGCCAATCAGTTGCCGTAAACCGGCAGCTTCTTGCAGATGGCCTTGACCTTCTCacggacgg                                                         >  6:222609/1‑149 (MQ=255)                                                                                                    tCTTTGTACGAGGGGCGGTGCGCCTGTATTAAACGAAAGAGCCCGGCACTGGGCCGGGCTCTTTCACTTTAGGCAAGCGCCAATCAGTTGCCGTAAACCGGCAGCTTCTTGCAGATGGCCTTGACCTTCTCACGGACGGCGTCGATCAc                                               >  7:86203/1‑149 (MQ=255)                                                                                                      tttGTACGAGGGGCGGTGCGCCTGTATTAAACGAAAGAGCCCGGCACTGGGCCGGGCTCTTTCACTTTAGGCAAGCGCCAATCAGTTGCCGTAAACCGGCAGCTTCTTGCAGATGGCCTTGACCTTCTCACGGACGGCGTc                                                     <  3:238269/141‑1 (MQ=255)                                                                                                      tttGTACGAGGGGCGGTGCGCCTGTATTAAACGAAAGAGCCCGGCACTGGGCCGGGCTCTTTCACTTTAGGCAAGCGCCAATCAGTTGCCGTAAACCGGCAGCTTCTTGCAGATGGCCTTGACCTTCTCACGGACGGCGTc                                                     >  4:238269/1‑141 (MQ=255)                                                                                                      tttGTACGAGGGGCGGTGCGCCTGTATTAAACGAAAGAGCCCGGCACTGGGCCGGGCTCTTTCACTTTAGGCAAGCGCCAATCAGTTGCCGTAAACCGGCAGCTTCTTGCAGATGGCCTTGACCTTCTCACGGACGGCGTCGATCACCg                                             >  6:190994/1‑149 (MQ=255)                                                                                                      tttGTACGAGGGGCGGTGCGCCTGTATTAAACGAAAGAGCCCGGCACTGGGCCGGGCTCTTTCACTTTAGGCAAGCGCCAATCAGTTGCCGTAAACCGGCAGCTTCTTGCAGATGGCCTTGACCTTCTCACGGACGGCGTCGATCACCg                                             >  3:113832/1‑149 (MQ=255)                                                                                                          tACGAGGGGCGGTGCGCCTGTATTAAACGAAAGAGCCCGGCACTGGGCCGGGCTCTTTCACTTTAGGCAAGCGCCAATCAGTTGCCGTAAACCGGCAGCTTCTTGCAGATGGCCTTGACCTTCTCACGGACGGCGTCGATCACCGCTTc                                         <  5:190994/149‑1 (MQ=255)                                                                                                            cGAGGGGCGGTGCGCCTGTATTAAACGAAAGAGCCCGGCACTGGGCCGGGCTCTTTCACTTTAGGCAAGCGCCAATCAGTTGCCGTAAACCGGCAGCTTCTTGCAGATGGCCTTGACCTTCTCACGGACGGCGTCGATCACCGCTTCgt                                       <  8:86203/149‑1 (MQ=255)                                                                                                               ggggCGGTGCGCGTGTATTAAACGAAAGAGCCCGGCACTGGGCCGGGCTCTTTCACTTTAGGCAAGCGCCAATCAGTTGCCGTAAACCGGCAGCTTCTTGCAGATGGCCTTGACCTTCTCACGGACGGCGTCGATCACCGCTTCgttgt                                    <  5:222609/149‑1 (MQ=255)                                                                                                                                  aaaCGAAAGAGCCCGGCACTGGGCCGGGCTCTTTCACTTTAGGCAAGCGCCAATCAGTTGCCGTAAACAGGCAGCTTCTTGCAGATGGCCTTGCCCTTCTCACGGACGGCGTCGATCACCGCTTCGTTGTTCAGGTCGGCCAGGATGTc                 >  3:93126/1‑149 (MQ=255)                                                                                                                                   aaCGAAAGAGCCCGGCACTGGGCCGGGCTCTTTCACTTTAGGCAAGCGCCAATCAGTTGCCGTAAACCGGCAGCTTCTTGCAGATGGCCTTGACCTTCTCACGTACGGCGTCGATCACCGCTTCGTTGTTCAGGTCGGCCAGGATGTc                 <  4:113832/148‑1 (MQ=255)                                                                                                                                   aaCGAAAGAGCCCGGCACTGGGCCGGGCTCTTTCACTTTAGGCAAGCGCCAATCAGTTGCCGTAAACCGGCAGCTTCTTGCAGATGGCCTTGACCTTCTCACGGACGGCGTCGATCACCGCTTCGTTGTTCAGGTCGGCCAGGATGTCg                >  5:313486/1‑149 (MQ=255)                                                                                                                                    gtgAAAGAGCCCGGCCCAGTGCCGGACTCTTTCGCTTTAGGCAAGCGCCAATCAGTTGCCGTAAACCGGCAGCTTCTTGCAGATGGCCTTGACCTTCTCACGGACGGCGTCGATCACCGCTTCGTTGTTCAGGTCGGCCAGGATGTCg                >  7:229647/3‑148 (MQ=255)                                                                                                                                    aCGAAAGAGCCCGGCACTGGGCCGGGCTCTTTCACTTTAGGCAAGCGCCAATCAGTTGCCGTAAACCGGCAGCTTCTTGCAGATGGCCTTGACCTTCTCACGGACGGCGTCGATCACCGCTTCGTTGTTCAGGTCGGCCAGGATGTCg                >  7:164400/1‑148 (MQ=255)                                                                                                                                    aCGAAAGAGCCCGGCACTGGGCCGGGCTCTTTCACTTTAGGCAAGCGCCAATCAGTTGCCGTAAACCGGCAGCTTCTTGCAGATGGCCTTGACCTTCTCACGGACGGCGTCGATCACCGCTTCGTTGTTCAGGTCGGCCAGGATGTCGc               <  8:150321/149‑1 (MQ=255)                                                                                                                                      gAAAGAGCCCGGCACTGGGCCGGGCTCTTTCACTTTAGGCAAGCGCCAATCAGTTGCCGTAAACCGGCAGCTTCTTGCAGATGGCCTTGACCTtctc                                                                 >  4:49431/1‑97 (MQ=255)                                                                                                                                      gAAAGAGCCCGGCACTGGGCCGGGCTCTTTCACTTTAGGCAAGCGCCAATCAGTTGCCGTAAACCGGCAGCTTCTTGCAGATGGCCTTGACCTtctc                                                                 <  3:49431/97‑1 (MQ=255)                                                                                                                                           agCCCGGCACTGGGCCGGGCTCTTTCACTTTAGGCAAGCGCCAATCAGTTGCCGTAAACCGGCAGCTTCTTGCAGATGGCCTTGACCTTCTCACGGACGGCGTCGATCACCGCTTCGTTGTTCAGGTCGGCCAGGATGTCGCAGATc          >  2:298771/1‑147 (MQ=255)                                                                                                                                            gCCCGGCCCAGTGCCGGGCTCTTTCACTTTAGGCAAGCGCCAATCAGTTGCCGTAAACCGGCAGCTTCTTGCAGATGGCCTTGACCTTCTCACGGACGGCGTCGATCACCGCTTCGTTGTTCAGGTCGGCCAGGATGTc                 >  6:98003/1‑139 (MQ=255)                                                                                                                                            gCCCGGCCCAGTGCCGGGCTCTTTCACTTTAGGCAAGCGCCAATCAGTTGCCGTAAACCGGCAGCTTCTTGCAGATGGCCTTGACCTTCTCACGGACGGCGTCGATCACCGCTTCGTTGTTCAGGTCGGCCAGGATGTc                 <  5:98003/139‑1 (MQ=255)                                                                                                                                                 gCACTGGGCCGGGCTCTTTCACTTTAGGCAAGCGCCAATCAGTTGCCGTAAACCGGCAGCTTCTTGCAGATGGCCTTGACCTTCTCACGGACGGCGTCGATCACCGCTTCGTTGTTCAGGTCGGCCAGGATGTCGCAGATCCAGCCGGc  <  3:26850/149‑1 (MQ=255)                                                                                                                                                     |                                                                                                                                                 CGGCCAGTACGGCGGCGAAGTAGATGGGGATCTGCCGGGTTTCGAGTTGCTCTCTGGTCAAGGTGCGGCCTGTGCAAAGTGGAGGTATTGGGGGCGCAGTCTTTGTACGAGGGGCGGTGCGCCTGTATTAAACGAAAGAGCCCGGCACTGGGCCGGGCTCTTTCACTTTAGGCAAGCGCCAATCAGTTGCCGTAAACCGGCAGCTTCTTGCAGATGGCCTTGACCTTCTCACGGACGGCGTCGATCACCGCTTCGTTGTTCAGGTCGGCCAGGATGTCGCAGATCCAGCCGGC  >  NC_002947/780410‑780702

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑