Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 813,200 T→G 8.8% intergenic (+107/+24) PP_0699 → / ← PP_t70 LysE family transporter/tRNA‑Met

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_002947813,2000TG 8.8% 167.2 / 7.9 57intergenic (+107/+24)PP_0699/PP_t70LysE family transporter/tRNA‑Met
Reads supporting (aligned to +/- strand):  ref base T (24/28);  new base G (2/3);  total (26/31)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.63e-01

GCTTGGCCTGGCTGTGGCGGTGACCGGAATACGTTGAGGCGACGATAGCAGCGCCGAAACCTGTGATTCGGCGCCCGGCTGCTGCGGGATTGGTTGGCGCGGGCATCAGCAACTTTTTCGCAGGCAAAGAAA‑AAGGGCTTACCTTGCGGTAAGCCCTTTGTCTTGTTTGGTGGCTACACAGGGACTTGAACCCCGGACCCCAGCATTATGAATGCTATGCTCTAACCAACTGAGCTATGTAGCCGATGGCGCGCATTATTCTCTTGAACGCCCCCTCTGTCAACACTCATT  >  NC_002947/813057‑813347
                                                                                                                                                |                                                                                                                                                   
gCTTGGCCTGGCTGTGGCGGTGACCGGAATACGTTGAGGCGACGATAGCAGCGCCGAAACCTGTGATTCGGCGCCCGGCTGCTGCGGGATTGGTTGGCGCGGGCATCAGCAACTTTTTCGCAGGCAAAGAAA‑AAGGGCTTACCTTGCgg                                                                                                                                                >  6:78723/1‑149 (MQ=255)
  ttGGCCTGGCTGTGGCGGTGACCGGAATACGTTGAGGCGACGATAGCAGCGCCGAAACCTGTGATTCGGCGCCCGGCTGCTGCGGGATTGGTTGGCGCGGGCATCAGCAACTTTTTCGCAGGCAAAGAAA‑AAGGGCTTACCTTGCGGt                                                                                                                                               >  4:133836/1‑148 (MQ=255)
     gCCTGGCTGTGGCGGTGACCGGAATACGTTGAGGCGACGATAGCAGCGCCGAAACCTGTGATTCGGCGCCCGGCTGCTGCGGGATTGGTTGGCGCGGGCATCAGCAACTTTTTCGCAGGCAAAGAAA‑AAGGGCTTACCTTGCGGTAAg                                                                                                                                            <  1:219652/148‑1 (MQ=255)
       cTGGCTGTGGCGGTGACCGGAATACGTTGAGGCGACGATAGCAGCGCCGAAACCTGTGATTCGGCGCCCGGCTGCTGCGGGATTGGTTGGCGCGGGCATCAGCAACTTTTTCGCAGGCAAAGAAA‑AAGGGCTTACCTTGCGGTaa                                                                                                                                             <  4:24706/145‑1 (MQ=255)
       cTGGCTGTGGCGGTGACCGGAATACGTTGAGGCGACGATAGCAGCGCCGAAACCTGTGATTCGGCGCCCGGCTGCTGCGGGATTGGTTGGCGCGGGCATCAGCAACTTTTTCGCAGGCAAAGAAA‑AAGGGCTTACCTTGCGGTaa                                                                                                                                             >  3:24706/1‑145 (MQ=255)
          gCTGTGGCGGTGACCGGAATACGTTGAGGCGACGATAGCAGCGCCGAAACCTGTGATTCAGCGCCCGGCTGCTGCGGGATTGGTTGGCGCGGGCATCAGCAACTTTTTCGCAGGCAAAGAAA‑AAGGGCTTACCTTGCGGTAAGCCCttt                                                                                                                                      <  3:329936/149‑1 (MQ=255)
                gCGGTGACCGGAATACGTTGAGGCGACGATAGCAGCGCCGAAACCTGTGATTCGGCGCCCGGCTGCTGCGGGATTGGTTGGCGCGGGCATCAGCAACTTTTTCGCAGGCAAAGAAA‑AAGGGCTTACCTTGCGGTAAGCCCTTTGTCtt                                                                                                                                 <  1:178371/148‑1 (MQ=255)
                gCGGTGACCGGAATACGTTGAGGCGACGATAGCAGCGCCGAAACCTGTGATTCGGCGCCCGGCTGCTGCGGGATTGGTTGGCGCGGGCATCAGCAACTTTTTCGCAGGCAAAGAAA‑AAGGGCTTACCTTGCGGTAAGCCCTTTGTCtt                                                                                                                                 >  2:178371/1‑148 (MQ=255)
                 cGGTGACCGGAATACGTTGAGGCGACGATAGCAGCGCCGAAACCTGTGATTCGGCGCCCGGCTGCTGCGGGATTGGTTGGCGCGGGCATCAGCAACTTTTTCGCAGGCAAAGAAA‑AAGGGCTTACCGCAAGGTAAGCCCTTTTTCtttg                                                                                                                               <  7:311056/149‑3 (MQ=39)
                 cGGTGACCGGAAGACGTTGAGGCGCCGCTAGCAGCGCCGAAACCTGTGATTCGGCGCCCGGCTGCGGCGGGATTGGTTGGCGCGGGCATCAGCAACTTTTTCGCAGGCAAAGAAA‑AAGGGCTTACCTTGCGGTATGCCCTTTGTCTTGt                                                                                                                               <  4:199038/149‑1 (MQ=255)
                      aCCGGAATACGTTGAGGCGACGATAGCAGCGCCGAAACCTGTGATTCGGCGCCCGGCTGCTGCGGGATTGGTTGGCGCGGGCATCAGCAACTTTTTCGCAGGCAAAGAAACAAGGGCTTACCTTGCGGTAAGCCCTTTGTCTTGTTtg                                                                                                                            <  1:238493/148‑1 (MQ=255)
                       ccGGAATACGTTGAGGCGACGATAGCAGCGCCGAAACCTGTGATTCGGCGCCCGGCTGCTGCGGGATTGGTTGGCGCGGGCATCAGCAACTTTTTCGCAGGCAAAGAAA‑AAGGGCTTACCTTGCGGTAAGCCCTTTGTCTTGTTtggtg                                                                                                                         <  5:78723/149‑1 (MQ=255)
                        cGGAATACGTTGAGGCGACGATAGCAGCGCCGAAACCTGTGATTCGGCGCCCGGCTGCTGCGGGATTGGTTGGCGCGGGCATCAGCAACTTTTTCGCAGGCAAAGAAA‑AAGGGCTTACCTTGCGGTAAGCCCTTTGTCTTGTTtggtgg                                                                                                                        >  5:11165/1‑149 (MQ=255)
                                gTTGAGGCGACGATAGCAGCGCCGAAACCTGTGATTCGGCGCCCGGCTGCTGCGGGATTGGTTGGCGCGGGCATCAGCAACTTTTTCGCAGGCAAAGAAA‑AAGGGCTTACCTTGCGGTAAGccc                                                                                                                                         <  1:39218/124‑1 (MQ=255)
                                gTTGAGGCGACGATAGCAGCGCCGAAACCTGTGATTCGGCGCCCGGCTGCTGCGGGATTGGTTGGCGCGGGCATCAGCAACTTTTTCGCAGGCAAAGAAA‑AAGGGCTTACCTTGCGGTAAGccc                                                                                                                                         >  2:39218/1‑124 (MQ=255)
                                      gCGACGATAGCAGCGCCGAAACCTGTGATTCGGCGCCCGGCTGCTGCGGGATTGGTTGGCGCGGGCATCAGCAACTTTTTCGCAGGCAAAGAAA‑AAGGGCTTACCTTGCGGTAAGCCCTATGTCTTGTTTGGTGGCTACACAGGGACtt                                                                                                          >  7:171440/1‑149 (MQ=255)
                                        gacgaTAGCAGCGCCGAAACCTGTGATTCGGCGCCCGGCTGCTGCGGGATTGGTTGGCGCGGGCATCAGCAACTTTTTCGCATGCAAAGAAA‑AAGGGCTTACCTTGCGGTAAGCCCTTTGTCTTGTTTGGTGGCTACACAg                                                                                                                <  8:73345/141‑1 (MQ=255)
                                        gacgaTAGCAGCGCCGAAACCTGTGATTCGGCGCCCGGCTGCTGCGGGATTGGTTGGCGCGGGCATCAGCAACTTTTTCGCAGGCAAAGAAA‑AAGGGCTTACCTTGCGGTAAGCCCTTTGTCTTGTTTGGTGGCTACACAg                                                                                                                >  7:73345/1‑141 (MQ=255)
                                          cgaTAGCAGCGCCGAAACCTGTGATTCGGCGCCCGGCTGCTGCGGGATTGGTTGGCGCGGGCATCAGCAACTTTTTCGCAGGCAAAGAAA‑AAGGGCTTACCTTGCGGTAAGCCCTATGTCTTGTTTGGTGGCTACACAGGGACTTGAAc                                                                                                      <  8:171440/149‑1 (MQ=255)
                                                  gcgcCGAAACCTGTGATTCGGCGCCCGGCTGCTGCGGGATTGGTTGGCGCGGGCATCAGCAACTTTTTCGCAGGCAAAGAAA‑AAGGGCTTACCTTGCGGTAAGCCCTTTGTCTTGTTTGGTGGCTACACAGGGACTTGAACCCCGGAcc                                                                                              <  6:11165/149‑1 (MQ=255)
                                                     ccGAAACCTGTGATTCGGCGCCCGGCTGCTGCGGGATTGGTTGGCGCGGGCATCAGCAACTTTTTCGCAGGCAAAGAAA‑AAGGGCTTACCTTGCGGTAAGCCCTTTGTCTTGTTTGGTGGCTACACAGGGACTTGaa                                                                                                       <  1:260371/137‑1 (MQ=255)
                                                     ccGAAACCTGTGATTCGGCGCCCGGCTGCTGCGGGATTGGTTGGCGCGGGCATCAGCAACTTTTTCGCAGGCAAAGAAA‑AAGGGCTTACCTTGCGGTAAGCCCTTTGTCTTGTTTGGTGGCTACACAGGGACTTGaa                                                                                                       >  2:260371/1‑137 (MQ=255)
                                                      cGAAACCTGTGATTCGGCGCCCGGCTGCTGCGGGATTGGTTGGCGCGGGCATCAGCAACTTTTTCGCAGGCAAAGACA‑AAGGGCTTACCGCAAGGTAAGCCCt                                                                                                                                        >  3:174990/1‑103 (MQ=38)
                                                      cGAAACCTGTGATTCGGCGCCCGGCTGCTGCGGGATTGGTTGGCGCGGGCATCAGCAACTTTTTCGCAGGCAAAGACA‑AAGGGCTTACCGCAAGGTAAGCCCt                                                                                                                                        <  4:174990/103‑1 (MQ=38)
                                                      cGAAACCTGTGATTCGGCGCCCGGCTGCTGCGGGATTGGTTGGCGCGGGCATCAGCAACTTTTTCGCAGGCAAAGAAA‑AAGGGCTTACCTTGCGGTAAGCCCTTTGTCTTGtt                                                                                                                              <  1:276347/113‑1 (MQ=255)
                                                      cGAAACCTGTGATTCGGCGCCCGGCTGCTGCGGGATTGGTTGGCGCGGGCATCAGCAACTTTTTCGCAGGCAAAGAAA‑AAGGGCTTACCTTGCGGTAAGCCCTTTGTCTTGTTTGGTGGCTACACAGGGACTTGAACCCCGGACCCCAg                                                                                          >  8:192825/1‑149 (MQ=255)
                                                      cGAAACCTGTGATTCGGCGCCCGGCTGCTGCGGGATTGGTTGGCGCGGGAATCAGCAACTTTTTCGCAGGCAAAGAAA‑AAGGGCTTACCTTGCGGTAAGCCCTTTGTCTTGtt                                                                                                                              >  2:276347/1‑113 (MQ=255)
                                                             tgtgATTCGGCGCCCGGCTGCTGCGGGATTGGTTGGCGCGGGCATCAGCAACTTTTTCGCAGGCAAAGAAA‑AAGGGCTTACCTTGCGGTAAGCCCTTTGTCTTGTTTGGTGGCTACACAggg                                                                                                              <  4:189292/122‑1 (MQ=255)
                                                             tgtgATTCGGCGCCCGGCTGCTGCGGGATTGGTTGGCGCGGGCATCAGCAACTTTTTCGCAGGCAAAGAAA‑AAGGGCTTACCTTGCGGTAAGCCCTTTGTCTTGTTTGGTGGCTACACAggg                                                                                                              >  3:189292/1‑122 (MQ=255)
                                                             tgtgATTCGGCGCCCGGCTGCTGCGGGATTGGTTGGCGCGGGCATCAGCAACTTTTTCGCAGGCAAAGAAA‑AAGGGCTTACCTTGCGGTAAGCCCTTTGTCTTGTTTGGTGGCTACACAGGGACTTGAACCCCGGACCCCAGCATTAt                                                                                    >  8:116134/1‑148 (MQ=255)
                                                                  ttCGGCGCCCGGCTGCTGCGGGATTGGTTGGCGCGGGCATCAGCAACTTTTTCGCAGGCAAAGAAA‑AAGGGCTTACCTTGCGGTAAGCCCTTTGTCTTGTTtgg                                                                                                                           <  7:214013/104‑1 (MQ=255)
                                                                  ttCGGCGCCCGGCTGCTGCGGGATTGGTTGGCGCGGGCATCAGCAACTTTTTCGCAGGCAAAGAAA‑AAGGGCTTACCTTGCGGTAAGCCCTTTGTCTTGTTtgg                                                                                                                           >  8:214013/1‑104 (MQ=255)
                                                                   tCGGCGCCCGGCTGCTGCGGGATTGGTTGGCGCGGGCATCAGCAACTTTTTCGCAGGCAAAGAAA‑AAGGGCTTACCTTGCGGTAAGCCCTTTGTCTTGTTTGGTGGCTACACAGGGACTTGAACCCCGGACCCCAGCATTATGAatgc                                                                              >  6:284530/1‑148 (MQ=255)
                                                                       cgcCCGGCTGCTGCGGGATTGGTTGGCGCGGGCATCAGCAACTTTTTCGCAGGCAAAGAAA‑AAGGGCTTACCTTGCGGTAAGCCCTTTGTCTTGTTTGGTGGCTACACAGGGACTTGAACCCCGGACCCCAGCATTATGAatgctatgc                                                                         <  6:197486/149‑1 (MQ=255)
                                                                        gcCCGGCTGCTGCGGGATTGGTTGGCGCGGGCATCAGCAACTTTTTCGCAGGCAAAGAAA‑AAGGGCTTACCTTGCGGTAAGCCCTTTGTCTTGTTTGGTGGCTACACAGGGACTTGCACCCCGGACCCCAGCATTATGAatgctatgct                                                                        >  7:41506/1‑149 (MQ=255)
                                                                          ccGGCTGCTGCGGGATTGGTTGGCGCGGGCATCAGCAACTTTTTCGCAGGCAAAGAAA‑AAGGGCTTACCTTGCGGTAAGCCCTTTGTCTTGTTTGGTGGCTACACAGGGACTTGAACCCCGGACCCCAGCATTATGAATGCTATGctc                                                                       >  4:216807/1‑148 (MQ=255)
                                                                                    cGGGATTGGTTGGCGCGGGCATCAGCAACTTTTTCGCAGGCAAAGAAA‑AAGGGCTTACCTTGCGGTAAGCCCTTTGTCTTGTTTGGTGGCTACACAGGGACTTGAACCCCGGACCCCAGCATTATGAATGCTATGCTCTAACCAACTg                                                             <  3:216807/148‑1 (MQ=255)
                                                                                     gggATTGGTTGGCGCGGGCATCAGCAACTTTTTCGCAGGCAAAGAAA‑AAGGGCTTACCTTGCGGTAAGCCCTTTGTCTTGTTTGGTGGCTACACAGGGACTTGAACCCCGGACCCCAGCATTATGAATGCTATGCTCTAACCAACTggg                                                           <  3:133836/149‑3 (MQ=255)
                                                                                      ggATTGGTTGGCGCGGGCATCAGCAACTTTTTCGCAGGCAAAGAAA‑AAGGGCTTACCTTGCGGTAAGCCCTTTGTCTTGTTTGGTGGCTACACAGGGACTTGAACCCCGGACCCCAGCATTATGAATGCTATGCTCTAACCAACTGAGc                                                          <  7:192825/149‑1 (MQ=255)
                                                                                          tggttggCGCGGGCATCAGCAACTTTTTCGCAGGCAAAGAAA‑AAGGGCTTACCTTGCGGTAAGCCCTTTGTCTTGTTTGGTGGCTACACAGGGACTTGAACCCCGGACCCCAGCATTATGAATGCTATGCTCTAACCAACTGAGCTAt                                                       <  7:116134/148‑1 (MQ=255)
                                                                                            gttggCGCGGGCATCAGCAACTTTTTCGCAGGCAAAGAAA‑AAGGGCTTACCTTGCGGTAAGCCCTTTGTCTTGTTTGGTGGCTACACAGGGACTTGAACCCCGGACCCCAGCATTAt                                                                                    >  4:85012/1‑117 (MQ=255)
                                                                                            gttggCGCGGGCATCAGCAACTTTTTCGCAGGCAAAGAAA‑AAGGGCTTACCTTGCGGTAAGCCCTTTGTCTTGTTTGGTGGCTACACAGGGACTTGAACCCCGGACCCCAGCATTAt                                                                                    <  3:85012/117‑1 (MQ=255)
                                                                                                         tCAGCAACTTTTTCGCAGGCAAAGAAA‑AAGGGCTTACCTTGCGGTAAGCCCTTTGTCTTGTTTGGTGGCTACACAGGGACTTGAACCCCGGACCCCAGCATTATGAATGCTATGCTCTAACCAACTGAGCTATGTAGCCGATGgcgcgc                                       <  8:41506/149‑1 (MQ=255)
                                                                                                           aGCAACTTTTTCGCAGGCAAAGAAA‑AAGGGCTTACCTTGCGGTAAGCCCTTTGTCTTGTTTGGTGGCTACACAGGGACTTGAACCCCGGACCCCAGCATTATGAATGCTATGctc                                                                       <  6:194195/115‑1 (MQ=255)
                                                                                                           aGCAACTTTTTCGCAGGCAAAGAAA‑AAGGGCTTACCTTGCGGTAAGCCCTTTGTCTTGTTTGGTGGCTACACAGGGACTTGAACCCCGGACCCCAGCATTATGAATGCTATGctc                                                                       >  5:194195/1‑115 (MQ=255)
                                                                                                           aGCAACTTTTTCGCAGGCAAAGAAA‑AAGGGCTTACCTTGCGGTAAGCCCTTTGTATTGTTTGGTGGCTACACAGGGACTTGAACCCCGGACCCCAGCATTATGAATGCTATGCTCTAACCAACTGAGCTATGTAGCCGATGGCGCGCat                                     <  5:284530/149‑1 (MQ=255)
                                                                                                            gCAACTTTTTCGCAGGCAAAGAAA‑AAGGGCTTACCTTGCGGTAAGCCCTTTGTCTTGTTTGGTGGCTACACAGGGACTTGAACCCCGGACCCCAGCATTATGAATGCTATGCTCTAACCAACTGAGCTATGTAGCCGATGGCGCGCat                                     >  3:28810/1‑148 (MQ=255)
                                                                                                                       gCAGGCAAAGAAA‑AAGGGCTTACCTTGCGGTAAGCCCTTTGTCTTGTTTGGTGGCTACACAGGGACTTGAACCCCGGCCCCCAGCATTATGAATGCTATGCTATAACCAACTGAGCTATGTGGCCGATGGCGCGCATTATTGTCTTGaa                         >  4:182057/1‑149 (MQ=255)
                                                                                                                       gCAGGCAAAGAAA‑AAGGGCTTACCTTGCGGTAAGCCCTTTGTCTTGTTTGGTGGCTACACAGGGACTTGAACCCCGGACCCCAGCa                                                                                        >  6:42850/1‑86 (MQ=255)
                                                                                                                       gCAGGCAAAGAAA‑AAGGGCTTACCTTGCGGTAAGCCCTTTGTCTTGTTTGGTGGCTACACAGGGACTTGAACCCCGGACCCCAGCa                                                                                        <  5:42850/86‑1 (MQ=255)
                                                                                                                           gCAAAGAAA‑AAGGGCTTTCCTTGCGGTAAGCCCTTTGTCTTGTTTGGTGGCTACACAGGGACTTGAACCCCGGACCCCAGCATTATGAATGCTATGCTCTAACCAACTGAGCTATGTAGCCGATGGCGCGCATTATTCTCTTGAACGcc                     <  3:182057/149‑1 (MQ=255)
                                                                                                                                gaca‑aaGGGCTTACCGCAAGGTAAGCCCTTTGTCTTGTTTGGTGGCTACACAGGGACTTGAACCCCGGACCCCAGCATTATGAatgctat                                                                           >  6:139019/4‑90 (MQ=37)
                                                                                                                                gaca‑aaGGGCTTACCGCAAGGTAAGCCCTTTGTCTTGTTTGGTGGCTACACAGGGACTTGAACCCCGGACCCCAGCATTATGAatgctat                                                                           <  5:139019/87‑1 (MQ=37)
                                                                                                                                       gggCTTACCTTGCGGTAAGCCCTTTGTCTTGTTTGGTGGCTACACAGGGACTTGAACCCCGGACCCCAGCATTATGAATGCTATGCTCTAACCAACTGAGCTATGTAGCCGATGGCGCGCATTATTCTCTTGAACGCCCCCTCTGTCaa          <  5:349616/149‑1 (MQ=255)
                                                                                                                                               cTTGCGGTAAGCCCTTTGTCTTGTTTGGTGGCTACACAGGGACTTGAACCCCGGACCCCAGCATTATGAATGCTATGCTCTAACCAACTGAGCTATGTAGCCGATGGCGCGCatt                                    <  1:342274/115‑1 (MQ=255)
                                                                                                                                               cTTGCGGTAAGCCCTTTGTCTTGTTTGGTGGCTACACAGGGACTTGAACCCCGGACCCCAGCATTATGAATGCTATGCTCTAACCAACTGAGCTATGTAGCCGATGGCGCGCatt                                    >  2:342274/1‑115 (MQ=255)
                                                                                                                                               cTTGCGGTAAGCCCTTTGTCTTGTTTGGTGGCTACACAGGGACTTGAACCCCGGACCCCAGCATTATGAATGCTATGCTCTAACCAACTGAGCTATGTAGCCGATGGCGCGCATTATTCTCTTGAACGCCCCCTCTGTCAACACTCAtt  >  5:282020/1‑149 (MQ=255)
                                                                                                                                                |                                                                                                                                                   
GCTTGGCCTGGCTGTGGCGGTGACCGGAATACGTTGAGGCGACGATAGCAGCGCCGAAACCTGTGATTCGGCGCCCGGCTGCTGCGGGATTGGTTGGCGCGGGCATCAGCAACTTTTTCGCAGGCAAAGAAA‑AAGGGCTTACCTTGCGGTAAGCCCTTTGTCTTGTTTGGTGGCTACACAGGGACTTGAACCCCGGACCCCAGCATTATGAATGCTATGCTCTAACCAACTGAGCTATGTAGCCGATGGCGCGCATTATTCTCTTGAACGCCCCCTCTGTCAACACTCATT  >  NC_002947/813057‑813347

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑