Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 816,930 G→A 7.4% P293P (CCG→CCA)  PP_0702 → MFS transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_002947816,9300GA 7.4% 157.2 / 2.2 54P293P (CCG→CCA) PP_0702MFS transporter
Reads supporting (aligned to +/- strand):  ref base G (26/24);  new base A (2/2);  total (28/26)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.64e-01

GCGACATGCTTTTCCAGCTGCCGCTGGGCTGGTTGGCCGATCGGGTTGAGCGCACAGGGCTGCACCTGGCGTGC‑GGGCTGGTGGCGATGGTGATCGGCATCGGTTTGCCTTGGCTGCTGAATCTGACCTGGCTGCTGTGGCCGCTGCTGGTGGTGCTGGGCGCGGTGGCGGGGGGGATCTATACCTTGGCGCTGGTGCTGATCGGGCAGCGCTTCAAGGGCCAGGACCTGGTCACTGCCAACGCCAGTGTGGGCTTGCTGTGGGGCGTAGGCAGCCTGGTCGGGCCGCTG  >  NC_002947/816788‑817077
                                                                                                                                               |                                                                                                                                                   
gCGACATGCTTTTCCAGCTGCCCCTGGGCTGGGTGGCCGAGCGGCTGCAGCGCACAGGGCTGCACCTGGCGTGC‑GGGCGGGTGGCGATGGTGATCGGCATCGGCTTGCCGTGGCCGCTGAAGCTGACCTGGCCGCTGTGGCCGctgctg                                                                                                                                               <  8:31455/149‑1 (MQ=255)
      aaccagcccAGCGGCAGCTGGGCTGGTTGGCCGATCGGGTTGAGCGCACAGGGCTGCACCTGGCGTGC‑GGGCTGGTGGCGATGGTGATCGGCATCGGTTTGCCTTGGCTGCTGAATCTGACCTGGCTGCTGTGGCCGCTGCTggtggt                                                                                                                                          <  1:206710/141‑1 (MQ=255)
      aaccagcccAGCGGCAGCTGGGCTGGTTGGCCGATCGGGTTGAGCGCACAGGGCTGCACCTGGCGTGC‑GGGCTGGTGGCGATGGTGATCGGCATCGGTTTGCCTTGGCTGCTGAATCTGACCTGGCTGCTGTGGCCGCTGCTggtggt                                                                                                                                          >  2:206710/8‑148 (MQ=255)
           ttCCAGCTGCCGCTGGGCTGGTTGGCCGATCGGGTTGAGCGCACAGGGCTGCACCTGGCGTGC‑GGGCTGGTGGCGATGGTGATCGGCATCGGTTTGCCTTGGCTGCTGAATCTGACCTGGCTGCTGTGGCCGCTGCTGGTGGTGCTg                                                                                                                                      <  5:58570/147‑1 (MQ=255)
              cAGCTGCCGCTGGGCTGGTTGGCCGATCGGGTTGAGCGCACAGGGCTGCACCTGGCGTGC‑GGGCTGGTGGCGATGGTGATCGGCATCGGTTTGCCTTGGCTGCTGAATCTGACCTGGCTGCTGTGGCCGCTGCTGGTGGTGCTGGgcgc                                                                                                                                 <  8:81153/149‑1 (MQ=255)
              cAGCTGCCGCTGGGCTGGTTGGCCGATCGGGTTGAGCGCACAGGGCTGCACCTGGCGTGC‑GGGCTGGTGGCGATGGTGATCGGCATCGGTTTGCCTTGGCTGCTGAATCTGACCTGGCTGCTGTGGCCGCTGCTGGTGGTGCTGGgcgc                                                                                                                                 >  4:316548/1‑149 (MQ=255)
                   gCCGCTGGGCTGGTTGGCCGATCGGGTTGAGCGCACAGGGCTGCACCTGGCGTGC‑GGGCTGGTGGCGATGGTGATCGGCATCGGTTTGCCTTGGCTGCTGAATCTGACCTGGCTGCTGTGGCCGCTGCtggtg                                                                                                                                            <  2:332143/133‑1 (MQ=255)
                   gCCGCTGGGCTGGTTGGCCGATCGGGTTGAGCGCACAGGGCTGCACCTGGCGTGC‑GGGCTGGTGGCGATGGTGATCGGCATCGGTTTGCCTTGGCTGCTGAATCTGACCTGGCTGCTGTGGCCGCTGCtggtg                                                                                                                                            >  1:332143/1‑133 (MQ=255)
                         gggctggTTGGCCGATCGGGTTGAGCGCACAGGGCTGCACCTGGCGTGC‑GGGCTGGTGGCGATGGTGATCGGCATCGGTTTGCCTTGGCTGCTGAATCTGACCTGGCTGCTGTGGCCGCTGCTGGTGGTGCTGGGCGCGGTGGCggggg                                                                                                                      <  8:206746/149‑1 (MQ=255)
                           gctggTTGGCCGATCGGGTTGAGCGCACAGGGCTGCACCTGGCGTGC‑GGGCTGGTGGCGATGGTGATCGGCATCGGTTTGCCTTGGCTGCTGAATCTGACCTGGCTGCTGTGGCCGCTGCTGGTGGTGCTGGGCGCGGTGGCggggggg                                                                                                                    >  4:40672/1‑149 (MQ=255)
                             tggTTGGCCGATCGGGTTGAGCGCACAGGGCTGCACCTGGCGTGC‑GGGCTGGTGGCGATGGTGATCGGCATCGGTTTGCCTTGGCTGCTGAATCTGACCTGGCTGCTGTGGCCGCTGCTGGTGGTGCTGGGCGCGGTGGCGGGGGGGAt                                                                                                                  <  3:316548/149‑1 (MQ=255)
                               gTTGGCCGATCGGGTTGAGCGCACAGGGCTGCACCTGGCGTGC‑GGGCTGGTGGCGATGGTGATCGGCATCGGTTTGCCTTGGCTGCTGAATCTGACCTGGCTGCTGTGGCCGCTGCTGGTGGTGCTGGGCGCGGTGGCGGGGGGGAt                                                                                                                  <  8:4439/147‑1 (MQ=255)
                                                   gcACAGGGCTGCACCTGGCGTGCGGGGCTGGTGGCGATGGTGATCGGCATCGGTTTGCCTTGGCTGCTGAATCTGACCTGGCTGCTGTGGCCGCTGCTGGTGGTGCTGGGCGCGGTGGCgggg                                                                                                                       >  1:179850/1‑123 (MQ=255)
                                                   gcACAGGGCTGCACCTGGCGTGC‑GGGCTGGTGGCGATGGTGATCGGCATCGGTTTGCCTTGGCTGCTGAATCTGACCTGGCTGCTGTGGCCGCTGCTGGTGGTGCTGGGCGCGGTGGCgggg                                                                                                                       <  2:179850/122‑1 (MQ=255)
                                                   gcACAGGGCTGCACCTGGCGTGC‑GGGCTGGTGGCGATGGTGATCGGCATCGGTTTGCCTTGGCTGCTGAATCTGACCTGGCTGCTGTGGCCACTGCTGGTGGTGCTGGGCGCGGTGGCGGGGGGGATCTATACCTTGGCGCTGGTGCt                                                                                             >  2:152824/1‑148 (MQ=255)
                                                   gcACAGGGCTGCACCTGGCGTGC‑GGGCTGGTGGCGATGGTGATCGGCATCGGTTTGCCTTGGCTGCTGAATCTGACCTGGCTGCTGTGGCCACTGCTGGTAGTGCTGGGCGCGGTGGCGGGGGGGATCTATACCTTGGCGCTGGTGCt                                                                                             >  2:147457/1‑148 (MQ=255)
                                                         ggCTGCACCTGGCGTGC‑GGGCTGGTGGCGATGGTGATCGGCATCGGTTTGCCTTGGCTGCTGAATCTGACCTGGCTGCTGTGGCCGCTGCTGGTGGTGCTGGGCGCGGTGGCGGGGGGGATCTATACCTTGgcg                                                                                                     >  4:192941/1‑134 (MQ=255)
                                                         ggCTGCACCTGGCGTGC‑GGGCTGGTGGCGATGGTGATCGGCATCGGTTTGCCTTGGCTGCTGAATCTGACCTGGCTGCTGTGGCCGCTGCTGGTGGTGCTGGGCGCGGTGGCGGGGGGGATCTATACCTTGgcg                                                                                                     <  3:192941/134‑1 (MQ=255)
                                                               aCCTGGCGTGC‑GGGCTGGTGGCGATGGTGATTGGCATCGGTTTGCCTTGGCTGCTGAATCTGACCTGGCTGCTGTGGCCGCTGCTGGTGGTGCTGGGCGCGGTGGCGGGGGGGATCTATACCTTGGCGCTGGTGCTGATCGGGCAgcgc                                                                                >  3:56788/1‑149 (MQ=255)
                                                                 cTGGCGTGC‑GGGCTGGTGGCGATGGTGATCGGCATCGGTTTGCCTTGGCTGCTGAATCTGACCTGGCTGCTGTGGCCGCTGCTGGTGGTGCTGGGCGCGGTGGCGGGGGGGATCTATACCTTGGCGCTGGTGCTGATCGGGCAGCGCtt                                                                              >  6:98642/1‑149 (MQ=255)
                                                                       tGC‑GGGCTGGTGGCGATGGTGATCGGCATCGGTTTGCCTTGGCTGCTGAATCTGACCTGGCTGCTGTGGCCGCTGCTGGTGGTGCTGGGCGCGGTGGCGGGGGGGATCTATACCTTGGCGCTGGTGCTGATCGGGCAGCGCTTCAAggg                                                                        <  5:98642/149‑1 (MQ=255)
                                                                           gggCTGGTGGCGATGGTGATCGGCATCGGTTTGCCTTGGCTGCTGAATCTGACCTGGCTGCTGTGGCCGCTGCTGGTGGTGCTGGGCGCGGTGGCGGGGGGGATCTATACCTTGGCGCTGGTGCTGATCGGGCAGCGCTTCAAg                                                                          >  5:37691/1‑144 (MQ=255)
                                                                           gggCTGGTGGCGATGGTGATCGGCATCGGTTTGCCTTGGCTGCTGAATCTGACCTGGCTGCTGTGGCCGCTGCTGGTGGTGCTGGGCGCGGTGGCGGGGGGGATCTATACCTTGGCGCTGGTGCTGATCGGGCAGCGCTTCAAg                                                                          <  6:37691/144‑1 (MQ=255)
                                                                                    gCGATGGTGATTGGCATCGGTTTGCCTTGGCTGCTGAATCTGACCTGGCTGCTGTGGCCGCTGCTGGTGGTGCTGGGCGCGGTGGCGGGGGGGATCTATACCTTGGCGCTGGTGCTGATCGGGCAGCGCTTCAAGGGCCAGGACCTGGt                                                            <  4:56788/149‑1 (MQ=255)
                                                                                    gCGATGGTGATCGGCATCGGTTTGCCTTGGCTGCTGAATCTGACCTGGCTGCTGTGGCCGCTGCTGGTGGTGCTGGGCGCGGTGGCGGGGGGGATCTATACCTTGGCGCTGGTGCTGATCGGGCAGCGCTTCAAGGGCCAGGACCTgg                                                             >  3:264202/1‑148 (MQ=255)
                                                                                    gCGATGGTGATCGGCATCGGTTTGCCTTGGCTGCTGAATCTGACCTGGCTGCTGTGGCCGCTGCTGGTGGTGCTGGGCGCGGTGGCGGGGGGGATCTATACCTTGGCGCTGGTGCTGATCGGGCAGCGCTTCAAGGGCCAGGACCTGGt                                                            >  3:23648/1‑149 (MQ=255)
                                                                                       aTGGTGATCGGCATCGGTTTGCCTTGGCTGCTGAATCTGACCTGGCTGCTGTGGCTGCTGCTGGTGGTGCTGGGCGCGGTGGCGGGGGGGATCTATACCTTGGCGCTGGTGCTGATCGGGCAGCGCTTCAAGGGCCAGGACCTGGTCAc                                                         >  8:126001/1‑149 (MQ=255)
                                                                                        tGGTGATCGGCATCGGTTTGCCTTGGCTGCTGAATCTGACCTGGCTGCTGTGGCTGCTGCTGGTGGTGCTGGGCGCGGTGGCGGGGGGGATCTATACCTTGGCGCTGGTGCTGATCGGGCAGCGCTTCAAGGGCCAGGACCTGGTCACt                                                        <  7:126001/149‑1 (MQ=255)
                                                                                             aTCGGCATCGGTTTGCCTTGGCTGCTGAATCTGACCTGGCTGCTGTGGCCGCTGCTGGTGGTGCTGGGCGCGGTGGCGGGGGGGATCTATACCTTGGCGCTGGTGCTGATCGGGCAGCGCTTCAAGGGCCAGGACCTGGTCACTGCCaa                                                   >  7:173510/1‑149 (MQ=255)
                                                                                                 gCATCGGTTTGCCTTGGCTGCTGAATCTGACCTGGCTGCTGTGGCCGCTGCTGGTGGTGCTGGGCGCGGTGGCGGGGGGGATCTATACCTTg                                                                                                        >  2:65314/1‑92 (MQ=255)
                                                                                                 gCATCGGTTTGCCTTGGCTGCTGAATCTGACCTGGCTGCTGTGGCCGCTGCTGGTGGTGCTGGGCGCGGTGGCGGGGGGGATCTATACCTTg                                                                                                        <  1:65314/92‑1 (MQ=255)
                                                                                                    tCGGTTTGCCTTGGCTGCTGAATCTGACCTGGCTGCTGTGGCCGCTGCTGGTGGTGCTGGGCGCGGTGGCGGGGGGGATCTATACCTTGGCGCTGGTGCTGATCGGGCAGCGCTTCAAGGGCCAGGACCTGGTCACTGCCAACGCCAgt                                            <  4:23648/149‑1 (MQ=255)
                                                                                                    tCGGTTTGCCTTGGCTGCTGAATCTGACCTGGCTGCTGTGGCCGCTGCTGGTGGTGCTGGGCGCGGTGGCGGGGGGGATCTATACCTTGGCGCTGGTGCTGATCGGGCAGCGCTTCAAGGGCCAGGACCTGGTCACTGCCAACGCCAgt                                            <  4:264202/149‑1 (MQ=255)
                                                                                                        tttGCCTTGGCTGCTGAATCTGACCTGGCTGCTGTGGCCACTGCTGGTTGTGCTGGGCGCGGTGGCGGGGGGGATCTATACCTTGGCGCTGGTGCTGATCGGGCAGCGCTTCAAGGGCCAGGACCTGGTCACTGCCAACGCCAGTGTgg                                        <  1:152824/149‑1 (MQ=255)
                                                                                                        tttGCCTTGGCTGCTGAATCTGACCTGGCTGCTGTGGCCACTGCTGGTGGTGCTGGGCGCGGTGGCGGGGGGGATCTATACCTTGGCGCTGGTGCTGATCGGGCAGCGCTTCAAGGGCCAGGACCTGGTCACTGCCAACGCCAGTGTgg                                        <  1:147457/149‑1 (MQ=255)
                                                                                                              ttGGCTGCTGAATCTGACCTGGCTGCTGTGGCCGCTGCTGGTGGTGCTGGGCGCGGTGGCGGGGGGGATCTATACCTTGGCGCTGGTGCTGATCGGGCAGCGCTTCAAGGGCCAGGACCTGGTCACTGCCAACGCCAGTGTGGGCTTGc                                  >  7:114443/1‑149 (MQ=255)
                                                                                                                   tgctgAATCTGACCTGGCTGCTGTGGCCGCTGCTGGTGGTGCTGGGCGCGGTGGCGGGGGGGATCTATACCTTGGCGCTGGTGCTGATCGGGCAGCGCTTCAAGGGCCAGGACCTGGTCACTGCCAACGCCAGTGTGGGCTTGCTGTgg                             >  7:9144/1‑149 (MQ=255)
                                                                                                                    gctgAATCTGACCTGGCTGCTGTGGCCGCTGCTGGTGGTGCTGGGCGCGGTGGCGGGGGGGATCTATACCTTGGCGCTGGTGCTGATCGGGCAGCGCTTCAAGGGCCAGGACCTGGTCACTGCCAACGCCAGTGTGGGCTTGCTGTggg                            >  1:336682/1‑149 (MQ=255)
                                                                                                                    gctgAATCTGACCTGGCTGCTGTGGCCGCTGCTGGTGGTGCTGGGCGCGGTGGCGGGGGGGATCTATACCTTGGCACTGGTGCTGATCGGGCAGCGCTTCAAGGGCCAGGACCTGGTCACTGCCAACGCCAGTGTGGGCTTGCTGTggg                            >  7:305512/1‑149 (MQ=255)
                                                                                                                     ctgAATCTGACCTGGCTGCTGTGGCCGCTGCTGGTGGTGCTGGGCGCGGTGGC‑GGGGGGATCTATACCTTGGCGCTGGTGCTGATCGGGCAGCGCTTCAAGGGCCAGGACCTGGTCACTGCCAACGCCAGTGTGGGCTTg                                   >  7:114343/1‑140 (MQ=255)
                                                                                                                     ctgAATCTGACCTGGCTGCTGTGGCCGCTGCTGGTGGTGCTGGGCGCGGTGGC‑GGGGGGATCTATACCTTGGCGCTGGTGCTGATCGGGCAGCGCTTCAAGGGCCAGGACCTGGTCACTGCCAACGCCAGTGTGGGCTTg                                   <  8:114343/140‑1 (MQ=255)
                                                                                                                      tgAATCTGACCTGGCTGCTGTGGCCGCTGCTGGTGGTGCTGGGCGCGGTGGCGGGGGGGATCTATACCTTGGCGCTGGTGCTGATCGGGCAGCGCTTCAAGGGCCAGGACCTGGTCACTGc                                                      <  2:65547/121‑1 (MQ=255)
                                                                                                                      tgAATCTGACCTGGCTGCTGTGGCCGCTGCTGGTGGTGCTGGGCGCGGTGGCGGGGGGGATCTATACCTTGGCGCTGGTGCTGATCGGGCAGCGCTTCAAGGGCCAGGACCTGGTCACTGc                                                      >  1:65547/1‑121 (MQ=255)
                                                                                                                        aaTCTGACCTGGCTGCTGTGGCCGCTGCTGGTGGTGCTGGGCGCGGTGGCGGGGGGGATCTATACCTTGGCGCTGGTGCTGATCGGGCAGCGCTTCAAGGGCCAGGACCTGGTCACTGCCAACGc                                                <  2:244614/125‑1 (MQ=255)
                                                                                                                        aaTCTGACCTGGCTGCTGTGGCCGCTGCTGGTGGTGCTGGGCGCGGTGGCGGGGGGGATCTATACCTTGGCGCTGGTGCTGATCGGGCAGCGCTTCAAGGGCCAGGACCTGGTCACTGCCAACGc                                                >  1:244614/1‑125 (MQ=255)
                                                                                                                        aaTCTGACCTGGCTGCTGTGGCCGCTGCTGGTGGTGCTGGGCGCGGTGGCGGGGGGGATCTATACCTTGGCGCTGGTGCTGATCGGGCAGCGCTTCAAGGGCCAGGACCTGGTCACTGCCAACGCCAgt                                            >  4:242207/1‑129 (MQ=255)
                                                                                                                               ccTGGCTGCTGTGGCCGCTGCTGGTGGTGCTGGGCGCGGTGGCGGGGGGGATCTATACCTTGGCACTGGTGCTGATCGGGCAGCGCTTCAAGGGCCAGGACCTGGTCACTGCCAACGCCAGTGTGGGCTTGCTGTGGGGCGTAGGCAGc                 <  8:305512/149‑1 (MQ=255)
                                                                                                                                 tGGCTGCTGTGGCCGCTGCTGGTGGTGCTGGGCGCGGTGGCGGGGGGGATCTATACCTTGGCGCTGGTGCTGATCGGGCAGCGCTTCAAGGGCCAGGACCTGGTCACTGCCAACGCCAGTGTGGGCTTGCTGTGGGGCGTAGGCAGCCt               >  1:86216/1‑149 (MQ=255)
                                                                                                                                  ggCTGCTGTGGCCGCTGCTGGTGGTGCTGGGCGCGGTGGCGGGGGGGATCTATACCTTGGCGCTGGTGCTGATCGGGCAGCGCTTCAAGGGCCAGGACCTGGTCACTGCCAACGCCAGTGTGGGCTTGCTGTGGGGCGTAGGCAGCCTg              >  6:7569/1‑149 (MQ=255)
                                                                                                                                   gctgctGTGGCCGCTGCTGGTGGTGCTGGGCGCGGTGGCGGGGGGGATCTATACCTTGGCGCTGGTGCTGATCGGGCAGCGCTTCAAGGGCCAGGACCTGGTCACTGCCAACGCCAGTGTGGGCTTGCTGTGGGGCGTAGGCAGCCTgg             <  2:336682/149‑1 (MQ=255)
                                                                                                                                          tgGCCGCTGCTGGTGGTGCTGGGCGCGGTGGCGGGGGGGATCTATACCTTGGCGCTGGTGCTGATCGGGCAGCGCTTCAAGGGCCAGGAGCTGGTCACTGCCAACGCCAGTGTGGGCTTGCTGTGGGGCGTAGGCAGCCTGGTCGGGc       <  8:114443/148‑1 (MQ=255)
                                                                                                                                            gCCGCTGCTGGTGGTGCTGGGCGCGGTGGCGGGGGGGATCTATACCTTGGCGCTGGTGCTGATCGGGCAGCGCTTCAAGGGCCAGGACCTGGTCACTGCCAACGCCAGTGTGGGCTTGCTGTGGGGCGTAGGCAGCCTGGTCggg        <  3:236312/145‑1 (MQ=255)
                                                                                                                                            gCCGCTGCTGGTGGTGCTGGGCGCGGTGGCGGGGGGGATCTATACCTTGGCGCTGGTGCTGATCGGGCAGCGCTTCAAGGGCCAGGACCTGGTCACTGCCAACGCCAGTGTGGGCTTGCTGTGGGGCGTAGGCAGCCTGGTCggg        >  4:236312/1‑145 (MQ=255)
                                                                                                                                              cGCTGCTGGTGGTGCTGGGCGCGGTGGCGGGGGGGATCTATACCTTGGCGCTGGTGCTGATCGGGCAGCGCTTCAAGGGCCAGGACCTGGTCACTGCCAACGCCAGTGTGGGCTTGCTGTGGGGCGTAGGCAGCCTGGTCGGGCCGCTg  >  3:185998/1‑149 (MQ=255)
                                                                                                                                               |                                                                                                                                                   
GCGACATGCTTTTCCAGCTGCCGCTGGGCTGGTTGGCCGATCGGGTTGAGCGCACAGGGCTGCACCTGGCGTGC‑GGGCTGGTGGCGATGGTGATCGGCATCGGTTTGCCTTGGCTGCTGAATCTGACCTGGCTGCTGTGGCCGCTGCTGGTGGTGCTGGGCGCGGTGGCGGGGGGGATCTATACCTTGGCGCTGGTGCTGATCGGGCAGCGCTTCAAGGGCCAGGACCTGGTCACTGCCAACGCCAGTGTGGGCTTGCTGTGGGGCGTAGGCAGCCTGGTCGGGCCGCTG  >  NC_002947/816788‑817077

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑