Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	821,679	G→C	9.5%	M350I (ATG→ATC)	PP_0707 →	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	821,679	0	G	C	9.5%	186.5 / 9.7	63	M350I (ATG→ATC)	PP_0707	hypothetical protein
Reads supporting (aligned to +/- strand):  ref base G (28/29);  new base C (3/3);  total (31/32)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.48e-01

GTGGTGTTCAGTGCCTTCATCACAGGCCTGGGCCGCGCCATGCTGATGCTGCAGCGTCCGTCCTGGCGGTTGCCGGCGATCCACGACGAAGTCGCCAGTGCGCTGGGCTGGTTCCCGAAGGTGCTGGCATTGGCACTGATGGTCATGATGACCATGGAGCGCATGAACAGCGTGATCGGTGCCAGCCTGGCGCTGACGGTGGCCGTCAACGGGCTGACCGCGCTGGTGGTGGCGCTCACCTTTGCAGGCGCGCTGCTGTGTTACCGCCGCACCCTGCGCAAGCATGACCTGGAG  >  NC_002947/821533‑821826                                                                                                                                                   |                                                                                                                                                    gtggtgTTCAGTGCCTTCATCACAGGCCTGGGCCGCGCCATGCTGATGCTGCAGCGTCCGTCCTGGCGGTTGCCGGCGATCCACGACGAAGTCGCCAGTGCGCTGGGCTGGTTCCCGAAGGTGCTGGCATTGGCACTGATGGTCatgat                                                                                                                                                   >  5:20865/1‑149 (MQ=255)  tggtgTTCAGTGCCTTCATCACAGGCCTGGGCCGCGCCATGCTGATGCTGCAGCGTCCGTCCTGGCGGTTGCCGGCGATCCACGACGAAGTCGCCAGTGCGCTGGGCTGGTTCCCGAAGGTGCTGGCATTGGCACTGATGGTCatgat                                                                                                                                                   >  5:188979/1‑148 (MQ=255)    gtgtTCAGTGCCTTCATCACAGGCCTGGGCCGCGCCATGCTGATGCTGCAGCGTCCGTCCTGGCGGTTGCCGGCGATCCACGACGAAGTCGCCAGTGCGCTGGGCTGGTTCCCGAAGGTGCTGGCATTGGCACTGATGGTCAtgatga                                                                                                                                                 <  5:92486/148‑1 (MQ=255)    gtgtTCAGTGCCTTCATCACAGGCCTGGGCCGCGCCATGCTGATGCTGCAGCGTCCGTCCTGGCGGTTGCCGGCGATCCACGACGAAGTCGCCAGTGCGCTGGGCTGGTTCCCGAAGGTGCTGGCATTGGCACTGATGGTCAtgatga                                                                                                                                                 <  1:73745/148‑1 (MQ=255)          aGTGCCTTCATCACAGGCCTGGGCCGCGCCATGCTGATGCTGCAGCGTCCGTCCTGGCGGTTGCCGGCGATCCACGACGAAGTCGCCAGTGCGATGGTCTGGTTCCCGAAGGTGCTGGCATTGGCACTGATGGTCATGATGACAATg                                                                                                                                            >  1:224600/1‑147 (MQ=255)           gTGCCTTCATCACAGGCCTGGGCCGCGCCATGCTGATGCTGCAGCGTCCGTCCTGGCGGTTGCCGGCGATCCACGACGAAGTCGCCAGTGCGCTGGGCTGGTTCCCGAAGGTGCTGGCATTGGCACTGATGGTCATGATGACCATGGAg                                                                                                                                         >  2:346233/1‑149 (MQ=255)           gTGCCTTCATCACAGGCCTGGGCCGCGCCATGCTGATGCTGCAGCGTCCGTCCTGGCGGTTGCCGGCGATCCACGACGAAGTCGCCAGTGCGCTGGGCTGGTTCCCAAAGGTGCTGGCATTGGCACTGATGGTCATGATGACCATGGAg                                                                                                                                         >  5:269107/1‑149 (MQ=255)            tGCCTTCATCACAGGCCTGGGCCGCGCCATGCTGATGCTGCAGCGTCCGTCCTGGCGGTTGCCGGCGATCCACGACGAAGTCGCCAGTGCGCTGGGCTGGTTCCCGAAGGTGCTGGCATTGGCACTGATGGTCATGATGACCATGGAgc                                                                                                                                        <  4:281164/149‑1 (MQ=255)              ccTTCATCACAGGCCTGGGCCGCGCCATGCTGATGCTGCAGCGTCCGTCCTGGCGGTTGCCGGCGATCCACGACGAAGTCGCCAGTGCGCTGGGCTGGTTCCCGAAGGTGCTGGCATTGGCACTGATGGTCATGATGACCATGGAgcg                                                                                                                                       >  7:166062/1‑148 (MQ=255)               cTTCATCACAGGCCTGGGCCGCGCCATGCAGATGCTGCAGCGTCCGTCCTGGCGGTTGCCGGCGATCCACGACGAAGTCGCCAGTGCGCTGGGCTGGTTCCCGAAGGTGCTGGCATTGGCACTGATGGTCATGATGACCATGGAGCGCa                                                                                                                                     >  3:225717/1‑149 (MQ=255)                ttCATCACAGGCCTGGGCCGCGCCATGCTGATGCTGCAGCGTCCGTCCTGGCGGTTGCCGGCGATCCACGACGAAGTCGCCAGTGCGCTGGGCTGGTTCCCGAAGGTGCTGGCATTGGCACTGATGGTCATGATGACCATGGAGCGCAt                                                                                                                                    >  8:326830/1‑149 (MQ=255)                  catcaCAGGCCTGGGCCGCGCCATGCTGATGCTGCAGCGTCCGTCCTGGCGGTTGCCGGCGATCCACGACGAAGTCGCCAGTGCGCTGGGCTGGTTCCCGAAGGTGCTGGCATTGGCACTGATGGTCATGATGACCATGGAGCGCATg                                                                                                                                   >  6:161642/1‑148 (MQ=255)                       caGGCCTGGGCCGCGCCATGCTGATGCTGCAGCGTCCGTCCTGGCGGTTGCCGGCGATCCACGACGAAGTCGCCAGTGCGCTGGGCTGGTTCCCGAAGGTGCTGGCATTGGCACTGATGGTCATGATGACCATGGAGCGCATGAACAGc                                                                                                                             >  1:314401/1‑149 (MQ=255)                       caGGCCTGGGCCGCGCCATGCTGATGCTGCAGCGTCCGTCCTGGCGGTTGCCGGCGATCCACGACGAAGTCGCCAGTGCGCTGGGCTGGTTCCCGAAGGTGCTGGCATTGGCACTGATGGTCATGATGACCATGGAGCGCATGAACAGc                                                                                                                             <  7:326830/149‑1 (MQ=255)                          gCCTGGGCCGCGCCATGCTGATGCTGCAGCGTCCGTCCTGGCGGTTGCCGGCGATCCACGACGAAGTCGCCAGTGCGCTGGGCTGGTTCCCGAAGGTGCTGGCATTGGCACTGATGGTCATGATGACCATGGAGCGCATGAACAGCGt                                                                                                                           >  6:190671/1‑148 (MQ=255)                           ccTGGGCCGCGCCATGCTGATGCTGCAGCGTCCGTCCTGGCGGTTGCCGGCGATCCACGACGAAGTCGCCAGTGCGCTGGGCTGGTTCCCGAAGGTGCTGGCATTGGCACTCATGGTCATCATGACCAtcagtg                                                                                                                                        <  5:184814/134‑6 (MQ=255)                           ccTGGGCCGCGCCATGCTGATGCTGCAGCGTCCGTCCTGGCGGTTGCCGGCGATCCACGACGAAGTCGCCAGTGCGCTGGGCTGGTTCCCGAAGGTGCTGGCATTGGCACTCATGGTAATCATGACCAtcagtg                                                                                                                                        >  6:184814/1‑129 (MQ=255)                                 ccGCGCCATGCTGATGCTGCAGCGTCCGTCCTGGCGGTTGCCGGCGATCCACGACGAAGTCACCAGTGCGCTGGGCTGGTTCCCGAAGGTGCTGGCATTGGCACTGATGGTCATGATGACCATGGAGCGCATGAAc                                                                                                                                >  1:115745/1‑136 (MQ=255)                                 ccGCGCCATGCTGATGCTGCAGCGTCCGTCCTGGCGGTTGCCGGCGATCCACGACGAAGTCACCAGTGCGCTGGGCTGGTTCCCGAAGGTGCTGGCATTGGCACTGATGGTCATGATGACCATGGAGCGCATGAAc                                                                                                                                <  2:115745/136‑1 (MQ=255)                                      ccATGCTGATGCTGCAGCGTCCGTCCTGGCGGTTGCCGGCGATCCACGACGAAGTCGCCAGTGCGCTGGGCTGGTTCCCGAAGGTGCTGGCATTGGCACTGATGGTCATGATGACCATGGAGCGCATGAACAGCGTGATCGGTGCCAGc                                                                                                              <  5:161642/149‑1 (MQ=255)                                         tgctgatgctgCAGCGTCCGTCCTGGCGGTTGCCGGCGATCCACGACGAAGTCGCCAGTGCGCTGGGCTGGTTCCCGAAGGTGCTGGCATTGGCACTGATGGTCATGATGACCATGGAGCGCATGAACAGCGTGATCGGTGCCAGCCTg                                                                                                           >  6:10287/1‑149 (MQ=255)                                         tgctgatgctgCAGCGTCCGTCCTGGCGGTTGCCGGCGATCCACGACGAAGTCGCCAGTGCGCTGGGCTGGTTCCCGAAGGTGCTGGCATTGGCACTGATGGTCATGATGACCATGGAGCGCATGAACAGCGTGATCGGTGCCAGCCTg                                                                                                           <  6:20865/149‑1 (MQ=255)                                         tgctgatgctgCAGCGTCCGTCCTGGCGGTTGCCGGCGATCCACGACGAAGTCGCCAGTGCGCTGGGCTGGTTCCCGAAGGTGCTGGCATTGGCACTGATGGTCATGATGACCATGGAGCGCATGAACAGCGTGATCGGTGCCAGCCTg                                                                                                           <  6:295649/149‑1 (MQ=255)                                         tgctgatgctgCAGCGTCCGTCCTGGCGGTTGCCGGCGATCCACGACGAAGTCGCCAGTGCGCTGGGCTGGTTCCCGAAGGTGCTGGCATTGGCACTGATGGTCATGATGACCATGGAGCGCATGAACAGCGTGATCGGTGCCAGCCTg                                                                                                           <  1:346233/149‑1 (MQ=255)                                           ctgatgctgCAGCGTCCGTCCTGGCGGTTGCCGGCGATCCACGACGAAGTCGCCAGTGCGCTGGGCTGGTTCCCGAAGGTGCTGGCATTGGCACTGATGGTCATGATGACCATGGAGCGCATGAACAGCGTGATCGGTGCCAGCCTgg                                                                                                          <  6:188979/148‑1 (MQ=255)                                             gatgctgCAGCGTCCGTCCTGGCGGTTGCCGGCGATCCACGACGAAGTCGCCAGTGCGCTGGGCTGGTTCCCGAAGGTGCTGGCATTGGCACTGATGGTCATGATGACCATGGAGCGCATGAACAGCGTGATCGGTGCCAGCCTGgcgc                                                                                                       >  8:67488/1‑149 (MQ=255)                                                gctgcAGCATCAG‑CATGGCGGTTGCCGGCGATCCACGACGAAGTCGCCAGTGCGCTGGGCTGGTTCCCGAAGGTGCTGGCATTGGCACTGATGGTCATGATGACCATGGAGCGCATGAACAGCGTGATCGGTGCCAGCCTGGCGCTGAc                                                                                                   <  8:148846/149‑1 (MQ=255)                                                     aGCGTCCGTCCTGGCGGTTGCCGGCGATCCACGACGAAGTCGCCAGTGCGCTGGGCTGGTTCCCGAAGGTGCTGGCATTGGCACTGATGGTCATGATGACCATGGAGCGCATGAACAGCGTGATCGGTGCCAGCCTGGCGCTGACGGTg                                                                                               <  3:93466/149‑1 (MQ=255)                                                      gCGTCCGTCCTGGCGGTTGCCGGCGATCCACGACGAAGTCGCCAGTGCGCTGGGCTGGTTCCCGAAGGTGCTGGCATTGGCACTGATGGTCATGATGACCATGGAGCGCATGAACAGCGTGATCGGTGCCAGCCTGGCGCTGACGGTgg                                                                                              <  7:67488/149‑1 (MQ=255)                                                      gCGTCCGTCCTGGCGGTTGCCGGCGATCCACGACGAAGTCGCCAGTGCGCTGGGCTGGTTCCCGAAGGTGCTGGCATTGGCACTGATGGTCATGATGACCATGGAGCGCATGAACAGCGTGATCGGTGCCAGCCTGGCGCTGACGGTgg                                                                                              <  4:225717/149‑1 (MQ=255)                                                       cgtccgtcCTGGCGGTTGCCGGCGATCCACGACGAAGTCGCCAGTGCGCTGGGCTGGTTCCCGAAGGTGCTGGCATTGGCACTGATGGTCATGATGACCATGGAGCGCATGAACAGCGTGATCGGTGCCAGCCTGGCGCTGACGGTGGc                                                                                             >  5:55987/1‑149 (MQ=255)                                                       cgtccgtcCTGGCGGTTGCCGGCGATCCACGACGAAGTCGCCAGTGCGCTGGGCTGGTTCCCGAAGGTGCTGGCATTGGCACTGATGGTCATGATGACCATGGAGCGCATGAACAGCGTGATCGGTGCCAGCCTGGCGCTGACGGTGGc                                                                                             >  4:296948/1‑149 (MQ=255)                                                        gtccgtccTGGCGGTTGCCGGCGATCCACGACGAAGTCGCCAGTGCGCTGGGCTGGTTCCCGAAGGTGCTGGCATTGGCACTGATGGTCATGATGACCATGGAGCGCATGAACAGCGTGATCGGTGCCAGCCTGGCGCTGACGGTGGcc                                                                                            <  8:166062/149‑1 (MQ=255)                                                         tccgtccTGGCGGTTGCCGGCGATCCACGACGAAGTCGCCAGTGCGCTGGGCTGGTTCCCGAAGGTGCTGGCATTGGCACTGATGGTCATGATGACCATGGAGCGCATGAACAGCGTGATCGGTGCCAGCCTGGCGCTGACGGTGGCCg                                                                                           >  6:308367/1‑149 (MQ=255)                                                         cccgtccTGGCGGGTGCCGGCGATCCACGACGAAGTCGCCAGTGCGCTGGGCTGGTTCCCGAAGGTGCTGGCATTGGCACTGATGGTCATGATGACCATGGAGCGCATGAACAGCGTGATCg                                                                                                                      <  6:5559/121‑1 (MQ=255)                                                           cgtccTGGCGGTTGCCGGCGATCCACGACGAAGTCGCCAGTGCGCTTGGCTGGTTCCCGAAGGTGCTGGCATTGGCACTGATGGTCATGATGACCATGGAGCGCATGAACAGCGTGATCGGTGCCAGCCTGGCGCTGACGGTGGCCGt                                                                                          <  2:224600/148‑1 (MQ=255)                                                           cgtccTGGCGGTTGCCGGCGATCCACGACGAAGTCGCCAGTGCGCTGGGCTGGTTCCCGAAGGTGCTGGCATTGGCACTGATGGTCATGATGACCATGGAGCGCATGAACAGCGTGATCGGTGCCAGCCTGGCGCTGACGGTGGCCGTc                                                                                         <  7:122938/149‑1 (MQ=255)                                                            gtccTGGCGGTTGCCGGCGATCCACGACGAAGTCGCCAGTGCGCTGGGCTGGTTCCCGAAGGTGCTGGCATTGGCACTGATGGTCATGATGACCATGGAGCGCATGAACAGCGTGATCGGTGCCAGCCTGGCGCTGACGGTGGCCGTc                                                                                         >  8:58484/1‑148 (MQ=255)                                                                tGGCGGTTGCCGGCGATCCACGACGAAGTCGCCAGTGCGCTGGGCTGGTTCCCGAAGGTGCTGGCATTGGCACTGATGGTCATGATGACCATGGAGCGCATGAACAGGGTGATCGGTGCCAGCCTGGCGCTGACGGTGGCCGTCAACgg                                                                                    >  5:306364/1‑149 (MQ=255)                                                                tGGCGGTTGCCGGCGATCCACGACGAAGTCGCCAGTGCGCTGGGCTGGTTCCCGAAGGTGCTGGCATTGGCACTGATGGTCATGATGACCATGGAGCGCATGAACAGCGTGATCGGTGCCAGCCTGGCGCTGACGGTGGCCGTCAACgg                                                                                    >  6:28894/1‑149 (MQ=255)                                                                  gCGGTTGCCGGCGATCCACGACGAAGTCGCCAGTGCGCTGGGCTGGTTCCCGAAGGTGCTGGCATTGGCACTGATGGTCATGATGACCATGGAGCGCATGAACAGCGTGATCGGTGCCAGCCTGGCGCTGACGGTGGCCGTCAACGGGc                                                                                  <  7:58484/149‑1 (MQ=255)                                                                    ggTTGCCGGCGATCCACGACGAAGTCGCCAGTGCGCTGGGCTGGTTCCCGAAGGTGCTGGCATTGGCACTGATGGTCATGATGACCATGGAGCGCAGGAACAGCGTGATCGGTGCCAGCCTGGCGCTGACGGTGGCCGTCAACGGGCTg                                                                                >  7:321222/1‑149 (MQ=255)                                                                        gCCGGCGATCCACGACGAAGTCGCCAGTGCGCTGGGCTGGTTCCCGAAGGTGCTGGCATTGGCACTGATGGTCATGATGACCATGGAGCGCATGAACAGCGTGATCGGTGCCAGCCTGGCGCTGACGGTGGCCGTCAACGGGCTGAcc                                                                             <  3:296948/148‑1 (MQ=255)                                                                             cGATCCACGACGAAGTCGCCAGTGCGCTGGGCTGGTTCCCGAAGGTGCTGGCATTGGCACTGATGGTCATGATGACCATGGAGCGCATGAACAGCGTGATCGGTGCCAGCCTGGCGCTGACGGTGGCCGTCAACGGGCTGACCGCGCtg                                                                       <  6:306364/149‑1 (MQ=255)                                                                                  cACGACGAAGTCGCCAGTGCGCTGGGCTGGTTCCCGAAGGTGCTGGCATTGGCACTGATGGTCATGATGACCATGGAGCGCATGAACAGCGTGATCGGTGCCAGCCTGGCGCTGACGGTGGCCGTCAACGGGCTGACCGCGCtggtggt                                                                  <  4:169459/149‑1 (MQ=255)                                                                                  cACGACGAAGTCGCCAGTGCGCTGGGCTGGTTCCCGAAGGTGCTGGCATTGGCACTGATGGTCATGATGACCATGGAGCGCATGAACAGCGTGATCGGTGCCAGCCTGGCGCTGACGGTGGCCGTCAACGGGCTGACCGCGCtggtggt                                                                  >  6:319586/1‑149 (MQ=255)                                                                                   acgacgAAGTCGCCAGTGCGCTGGGCTGGTTACCGAAGGTGCTGGCATTGGCACTGATGGTCATGATGACCATGGAGCGCATGAACAGCGTGATCGGTGCCAGCATGGCGCTGACGGTGGCCGTCAACGGGCTGACCGCGCtggtggtg                                                                 >  7:54303/1‑149 (MQ=255)                                                                                        gaAGTCGCCAGTGCGCTGGGCTGGTTCCCGAAGGTGCTGGCATTGGCACTGATGGTCATGATGACCATGGAGCGCATGAACAGCGTGATCGGTGCCAGCCTGGCGCTGACGGTGGCCGTCAACGGGCTGACCGCGCTGGTGGTGGCGCt                                                            <  5:28894/149‑1 (MQ=255)                                                                                              gCCAGTGCGCTGGGCTGGTTCCCGAAGGTGCTGGCATTGGCACTGATGGTCATGATGACCATGGAGCGCATGAACAGCGTGATCGGTGCCAGCCTGGCGCTGACGGTGGCCGTCAACGGGCTGACCGCGCTGGTGGTGGCGCTCACCtt                                                      <  6:55987/149‑1 (MQ=255)                                                                                                       ctgggctggTTCCCGAAGGTGCTGGCATTGGCACTGATGGTCATGATGACCATGGAGCGCATGAACAGCGTGATCGGTGCCAGCCTGGCGCTGACGGTGGCCGTCAACGGGCTGACCGCGCTGGTGGTGGCGCTCACCTTTGCAGgcgc                                             <  5:10287/149‑1 (MQ=255)                                                                                                          ggctggTTCCCAAAGGTGCTGGCATTGGCACTGATGGTCATGATGACCATGGAGCGCATGAACAGCGTGATCGGTGCCAGCCTGGCGCTGACGGTGGCCGTCAACGGGCTGACCGCGCTGGTGGTGGCGCTCACCTTTGCAGGCGCgct                                          <  6:269107/149‑1 (MQ=255)                                                                                                           gctggTTCCCGAAGGTGCTGGCATTGGCACTGATGGTCATGATGACCATGGAGCGCATGAACAGCGTGATCGGTGCCAGCCTGGCGCTGACGGTGGCCGTCAACGGGCTGACCGCGCTGGTGGTGGCGCTCACCTTTGCAGGCGCgctg                                         >  5:217009/1‑149 (MQ=255)                                                                                                              ggcgcccGAACGTGCTGGCATTGGCACTGATGGTCATGATGACCATGGAGCGCATGAACACCGTGATCGGTGCCAGCCTGGCGCTGACGGTGGCCGTCAACGGGCTGACCGCGCTGGTGGTGGCGCTCACCTTTGCAGGCGCGctgctg                                      <  8:321222/145‑1 (MQ=255)                                                                                                               gTTCCCGAAGGTGCTGGCATTGGCACTGATGGTCATGATGACCATGGAGCGCATGAACAGCGTGATCGGTGCCAGCCTGGCGCTGACGGTGGCCGTCAACGGGCTGACCGCGCTGGTGGTGGCGCTCACCTTTGCAGGCGCGCTGCtgt                                     >  1:117759/1‑149 (MQ=255)                                                                                                                  cccGAAGGTGCTGGCATTGGCACTGATGGTCATGATGACCATGGAGCGCATGAACAGCGTGATCGGTGCCAGCCTGGCGCTGACGGTGGCCGTCAACGGGCTGACCGCGCTGGTGGTGGCGCTCACCTTTGCAGGCGCGCTGCtgt                                     >  6:118845/1‑146 (MQ=255)                                                                                                                  cccGAAGGTGCTGGCATTGGCACTGATGGTCATGATGACCATGGAGCGCATGAACAGCGTGATCGGTGCCAGCCTGGCGCTGACGGTGGCCGTCAACGGGCTGACCGCGCTGGTGGTGGCGCTCACCTTTGCAGGCGCGCTGCtgt                                     <  5:118845/146‑1 (MQ=255)                                                                                                                   ccGAAGGTGCTGGCATTGGCACTGATGGTCATGATGACCATGGAGCGCATGAACAGCGTGATCGGTGCCAGCCTGGCGCTGACGGTGGCCGTCAACGGGCTGACCGCGCTGGTGGTGGCGCTCACCTTTGCAGGCGCGCTGCTGTGTTa                                 <  6:217009/149‑1 (MQ=255)                                                                                                                    cGAAGGTGCTGGCATTGGCACTGATGGTCATGATGACCATGGAGCGCATGAACAGCGTGATCGGTGCCAGCCTGGCGCTGACGGTGGCCGTCAACGGGCTGACCGCGCTGGTGGTGGCGCTCACCTTTGCAGGCGCGCTGCTGTGTTAc                                >  5:353852/1‑149 (MQ=255)                                                                                                                             tGGCATTGGCACTGATGGTCATGATGACCATGGAGCGCATGAACAGCGTGATCGGTGCCAGCCTGGCGCTGACGGTGGCCGTCAACGGGCTGACCGCGCTGGTGGTGGCGCTCACCTTTGCAGGCGCGCTGCTGTGTTACCGCCGCAcc                       >  3:227604/1‑149 (MQ=255)                                                                                                                               gCATTGGCACTGATGGTCATGATGACCATGGAGCGCATGAACAGCGTGATCGGTGCCAGCCTGGCGCTGACGGTGGCCGTCAACGGGCTGACCGCGCTGGTGGTGGCGCTCACCTTTGCAGGCGCGCTGCTGTGTTACCGCCGCACCCt                     >  8:156368/1‑149 (MQ=255)                                                                                                                               gCATTGGCACTGATGGTCATGATGACCATGGAGCGCATGAACAGCGTGATCGGTGCCAGCCTGGCGCTGACGGTGGCCGTCAACGGGCTGACCGCGCTGGTGGTGGCGCTCACCTTTGCAGGCGCGCTGCTGTGTTACCGCCGCACCCt                     >  1:189760/1‑149 (MQ=255)                                                                                                                                      gctccaTGGTCATCATGACCATGGAGCGCATGAACAGCGTGATCGGTGCCAGCCTGGCGCTGACGGTGGCCGTCAACGGGCTGACCGCGCTGGTGGTGGCGCTCACCTTTGCAGGCGCGCTGCTGTGtt                                  >  7:313430/6‑129 (MQ=255)                                                                                                                                      gctccaTGGTCATCATGACCATGGAGCGCATGAACAGCGTGATCGGTGCCAGCCTGGCGCTGACGGTGGCCGTCAACGGGCTGACCGCGCTGGTGGTGGCGCTCACCTTTGCAGGCGCGCTGCTGTGtt                                  <  8:313430/124‑1 (MQ=255)                                                                                                                                       ctccaTGGTCATCATGACCATGGAGCGCATGAACAGCGTGATCGGTGCCAGCCTGGCGCTGACGGTGGCCGTCAACGGGCTGACCGCGCTGGTGGTGGCGCTCACCTTTGCAGGCGCGCTGCTGTGTTACCGCCGCACCCTGCGCAAg              >  5:84537/5‑148 (MQ=255)                                                                                                                                       ctccaTGGTCATCATGACCATGGAGCGCATGAACAGCGTGATCGGTGCCAGCCTGGCGCTGACGGTGGCCGTCAACGGGCTGACCGCGCTGGTGGTGGCGCTCACCTTTGCAGGCGCGCTGCTGTGTTACCGCCGCACCCTGCGCAAGc             <  6:84537/145‑1 (MQ=255)                                                                                                                                       aCTGATGGTCATGATGACCATGGAGCGCATGAACAGCGTGATCGGTGCCAGCCTGGCGCTGACGGTGGCCGTCAACGGGCTGACCGCGCTGGTGGTGGCGCTCACCTTTGCAGTCGCGCTGCTGTGTTACCGCCGCACCCTGCGCAAGc             <  8:54303/149‑1 (MQ=255)                                                                                                                                            tGGTCATGATGACCATGGAGCGCATGAACAGCGTGATCGGTGCCAGCCTGGCGCTGACGGTGGCCGTCAACGGGCTGACCGCGCTGGTGGTGGCGCTCACCTTTGCAGGCGCGCTGCTGTGTTACCGCCGCACCCTGCGCAAGCATGAc        <  5:308367/149‑1 (MQ=255)                                                                                                                                                  tgatgaCCATGGAGCGCATGAACAGCGTGATCGGTGCCAGCCTGGCGCTGACGGTGGCCGTCAACGGGCTGACCGCGCTGGTGGTGGCGCTCACCTTTGCAGGCGCGCTGCTGTGTTACCGCCGCACCCTGCGCAAGCATGACCTGGAg  >  3:97103/1‑149 (MQ=255)                                                                                                                                                   |                                                                                                                                                    GTGGTGTTCAGTGCCTTCATCACAGGCCTGGGCCGCGCCATGCTGATGCTGCAGCGTCCGTCCTGGCGGTTGCCGGCGATCCACGACGAAGTCGCCAGTGCGCTGGGCTGGTTCCCGAAGGTGCTGGCATTGGCACTGATGGTCATGATGACCATGGAGCGCATGAACAGCGTGATCGGTGCCAGCCTGGCGCTGACGGTGGCCGTCAACGGGCTGACCGCGCTGGTGGTGGCGCTCACCTTTGCAGGCGCGCTGCTGTGTTACCGCCGCACCCTGCGCAAGCATGACCTGGAG  >  NC_002947/821533‑821826

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑