Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	852,166	G→C	7.5%	A315A (GCG→GCC) ‡	hemA →	glutamyl‑tRNA reductase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	852,166	0	G	C	7.5%	159.2 / 3.6	53	A315A (GCG→GCC) ‡	hemA	glutamyl‑tRNA reductase
Reads supporting (aligned to +/- strand):  ref base G (23/26);  new base C (2/2);  total (25/28)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.46e-01

AGCGCCGACACAAGCCCATCTTCATGGTCGACATCGCTGTACCTCGCGATATCGAAACCGAAGTCGGCGAGCTGGATGATGTCTACCTGTACACCGTCGACGACCTGCACGATGTAGTGGCGGAAAACCTCAAGAGCCGCCAGGGCGCGGCCCAGGCGGCCGAAGAGCTGGTATCCGTGGGCGCCGAAGACTTCATGCTGCGCCTGCGCGAACTGGCTGCAGTGGACGTGCTCAGGGCCTATCGCCAGCAAAGTGAGCGCCTTCGCGACGAAGAACTGCAAAAGGCCTTGCGCCT  >  NC_002947/852018‑852312                                                                                                                                                     |                                                                                                                                                   aGCGCCGACACAAGCCCATCTTCATGGTCGACATCGCTGTACCTCGCGATATCGAAACCGAAGTCGGCGAGCTGGATGATGTCTACCTGTACACCGTCGACGACCTGCACGATGTAGTGGCGGAAAACCTCAAGAGCCGCCAGGGcgcg                                                                                                                                                    <  7:267483/149‑1 (MQ=255) aGCGCCGACACAAGCCCATCTTCATGGTCGACATCGCTGTACCTCGCGATATCGAAACCGAAGTCGGCGAGCTGGATGATGTCTACCTGTACACCGTCGACGACCTGCACGATGTAGTGGCGGAAAACCTCAAGAGCCGCCAGGGcgcg                                                                                                                                                    <  1:331104/149‑1 (MQ=255)  gcgcCGACACAAGCCCATCTTCATGGTCGACATCGCTGTACCTCGCGATATCGAAACCGAAGTCGGCGAGCTGGATGATGTCTACCTGTACACCGTCGACGACCTGCACGATGTAGTGGCGGAAAACCTCAAGAGCCGCCAGGGCGCgg                                                                                                                                                   <  1:318153/149‑1 (MQ=255)                ccATCTTCATGGTCGACATCGCTGTACCTCGCGATATCGAAACCGAAGTCGGCGAGCTGGATGATGTCTACCTGTACACCGTCGACGACCTGCACGATGTAGTGGCGGAAAACCTCAAGAGCCGCCAGGGCGCGGCCCAGGCGGCCGaa                                                                                                                                     <  1:115555/149‑1 (MQ=255)                ccATCTTCATGGTCGACATCGCTGTACCTCGCGATATCGAAACCGAAGTCGGCGAGCTGGATGATGTCTACCTGTACACCGTCGACGACCTGCACGATGTAGTGGCGGAAAACCTCAAGAGCCGCCAGGGCGCGGCCCAGGCGGCCGaa                                                                                                                                     <  3:327438/149‑1 (MQ=255)                 cATCTTCATGGTCGACATCGCTGTACCTCGCGATATCGAAACCGAAGTCGGCGAGCTGGATGATGTCTACCTGTACACCGTCGACGACCTGCACGATGTAGTGGCGGAAAACCTCAAGAGCCGCCAGGGCGCGGCCCAGGCGGCCg                                                                                                                                       >  5:89262/1‑146 (MQ=255)                 cATCTTCATGGTCGACATCGCTGTACCTCGCGATATCGAAACCGAAGTCGGCGAGCTGGATGATGTCTACCTGTACACCGTCGACGACCTGCACGATGTAGTGGCGGAAAACCTCAAGAGCCGCCAGGGCGCGGCCCAGGCGGCCGaa                                                                                                                                     <  6:89262/148‑1 (MQ=255)                  aTCTTCATGGTCGACATCGCTGTACCTCGCGATATCGAAACCGAAGTCGGCGAGCTGGATGATGTCTACCTGTACACCGTCGACGACCTGCACGATGTAGTGGCGGAAAACCTCAAGAGCCGCCAGGGCGCGGCCCAGGCGGCCGaa                                                                                                                                     >  2:328285/1‑147 (MQ=255)                       cATGGTCGACATCGCTGTACCTCGCGATATCGAAACCGAAGTCGGCGAGCTGGATGATGTCTACCTGTACACCGTCGACGACCTGCACGATGTAGTGGCGGAAAACCTCAAGAGCCGCCAGGGCGCGGCCCAGGCGGCCGAAGAGCTg                                                                                                                               <  7:276118/148‑1 (MQ=255)                       cATGGTCGACATCGCTGTACCTCGCGATATCGAAACCGAAGTCGGCGAGCTGGATGATGTCTACCTGTACACCGTCGACGACCTGCACGATGTAGTGGCGGAAAACCTCAAGAGCCGCCAGGGCGCGGCCCAGGCGGCCGAAGAGCTg                                                                                                                               <  1:328285/148‑1 (MQ=255)                       cATGGTCGACATCGCTGTACCTCGCGATATCGAAACCGAAGTCGGCGAGCTGGATGATGTCTACCTGTACACCGTCGACGACCTGCACGATGTAGTGGCGGAAAACCTCAAGAGCCGCCAGGGCGCGGCCCAGGCGGCCGAAGAGCTg                                                                                                                               <  8:331868/148‑1 (MQ=255)                        aTGGTCGACATCGCTGTACCTCGCGATATCGAAACCGAAGTCGGCGAGCTGGATGATGTCTACCTGTACACCGTCGACGACCTGCACGATGTAGTGGCGGAAAACCTCAAGAGCCGCCTGGGCCGCGCCCTggcggc                                                                                                                                         <  2:248916/137‑1 (MQ=255)                        aTGGTCGACATCGCTGTACCTCGCGATATCGAAACCGAAGTCGGCCAGCTGGATGATGTCTACCTGTACACCGTCGACGACCTGCACGATGTAGTGGCGGAAAACCTCAAGAGCCGCCTGGGCCGCGCCCTggcggc                                                                                                                                         >  1:248916/1‑137 (MQ=255)                               aCATCGCTGTACCTCGCGATATCGAAACCGAAGTCGGCGAGCTGGATGATGTCTACCTGTACACCGTCGACGACCTGCACGATGTAGTGGCGGAAAACCTCAAGAGCCGCCAGGGCGCGGCCCAGGCGGCCGAAGATCTGGTATACGTg                                                                                                                      >  3:221603/1‑149 (MQ=255)                               aCATCGCTGTACCTCGCGATATCGAAACCGAAGTCGGCGAGCTGGATGATGTCTACCTGTACACCGTCGACGACCTGCACGATGTAGTGGCGGAAAACCTCAAGAGCCGCCAGGGCGCGGCCCAGGCGGCCGAAGAGCTGGTATCCGTg                                                                                                                      >  5:17077/1‑149 (MQ=255)                                     cTGTACCTCGCGATATCGAAACCGAAGTCGGCGAGCTGGATGATGTCTACCTGTACACCGTCGACGACCTGCACGATGTAGTGGCGGAAAACCTCAAGAGCCGCCAGGGCGCGGCCCAGGCGGCCGAAGAGCTGGTATCCGTGGGCGcc                                                                                                                >  5:63284/1‑149 (MQ=255)                                       gTACCTCGCGATATCGAAACCGAAGTCGGCGAGCTGGATGATGTCTACCTGTACACCGTCGACGACCTGCACGATGTAGTGGCGGAAAACCTCAAGAGCCGCCAGGGCGCGGCCCAGGCGGCCGAAGAGCTGGTATCCGTGGGCGCCg                                                                                                               >  2:3196/1‑148 (MQ=255)                                                 atgtCGAAACCGAAGTCGGCGAGCTGGATGATGTCTACCTGTACACCGTCGACGACCTGCACGATGTAGTGGCGGAAAACCTCAAGAGCCGCCAGGGCGCGGCCCAGGCGGCCGAAGAGCTGGTATCCGTGGGCGCCGAAGACTTCAtg                                                                                                    <  4:230484/146‑1 (MQ=255)                                                  tatCGAAACCGAAGTCGGCGAGCTGGATGATGTCTACCTGTACACCGTCGACGACCTGCACGATGTAGTGGCGGAAAACCTCAAGAGCCGCCAGGGCGCGGCCCAGGCGGcc                                                                                                                                        <  8:308120/112‑1 (MQ=255)                                                  tatCGAAACCGAAGTCGGCGAGCTGGATGATGTCTACCTGTACACCGTCGACGACCTGCACGATGTAGTGGCGGAAAACCTCAAGAGCCGCCAGGGCGCGGCCCAGGCGGcc                                                                                                                                        >  7:308120/1‑112 (MQ=255)                                                    tCGAAACCGAAGTCGGCGAGCTGGATGATGTCTACCTGTACACCGTCGACGACCTGCACGATGTAGTGGCGGAAAACCTCAAGAGCCGCCAGGGCGCGGCCCAGGCGGCCGAAGAGCTGGTATCCGTGGGCGCCGAAGACTTCAtgctg                                                                                                 >  3:55254/1‑149 (MQ=255)                                                      gAAACAGAAGTCGGCGAGCTGGGTGATGTCTACCTGTACACCGTCGACGACCTGCACGATGTAGTGGCGGAAAACCTCAAGAGCCGCCAGGGCGCGGCCCAGGCGGCCGAAGAGCTGGTATCCGTGGGCGCCGAAGACTTCATGctgcg                                                                                               >  2:306269/1‑149 (MQ=255)                                                         aCCGAAGTCGGCGAGCTGGATGATGTCTACCTGTACACCGTCGACGACCTGCACGATGTAGTGGCGGAAAACCTCAAGAGCCGCCAGGGCGCGGCCCAGGCGGCCGAAGAGCTGGTATCCGTGGGCGCCGAAGACTTCa                                                                                                      >  6:82796/1‑139 (MQ=255)                                                         aCCGAAGTCGGCGAGCTGGATGATGTCTACCTGTACACCGTCGACGACCTGCACGATGTAGTGGCGGAAAACCTCAAGAGCCGCCAGGGCGCGGCCCAGGCGGCCGAAGAGCTGGTATCCGTGGGCGCCGAAGACTTCa                                                                                                      <  5:82796/139‑1 (MQ=255)                                                                    cGAGCTGGATGATGTCACCCTGTACCCCGTCGACGACCTCCACGATGTAGTGGCGTAAAACCTCAAGCGCCGCCAGGGCGCGGCCCAGGCGGCCTAAGAGCTGGTATCCGTGGGCGCCGAAGACTTCATGCTGCGCCTGCGCGAACTgg                                                                                 <  7:292168/149‑1 (MQ=255)                                                                     gAGCTGGATGATGTCTACCTGTACACCGTCGACGACCTGCACGATGTAGTGGCGGAAAACCTCAAGAGCCGCCAGGGCGCGGCCCAGGCGGCCGAAGAGCTGGTATCCGTGGGCGCCGAAGACTTCATGCTGCGCCTGCGCGAACTGGc                                                                                <  6:17077/149‑1 (MQ=255)                                                                       gCTGGATGATGTCTACCTGTACACCGTCGACGACCTGCACGATGTAGTGGCGGAAAACCTCAAGAGCCGCCAGGGCGCGGCCCAGGCGGCCGAAGAGCTGGTATCCGTGGGCGCCGAAGACTTCATGCTGCGCCTGCGCGAACTGGCTg                                                                              >  5:146689/1‑149 (MQ=255)                                                                           ggtgatgTCTACCTGTACACCGTCGACGAACTGCACGATGTAGTGGCGGAAAACCTCAAGAGCCGCCAGGGCGCGGCCCAGGCGGCCGAAGAGCTGGTATCCGTGGGCGCCGAAGACTTCATGCTGCGCCTGCGCGAACTGGCTGCa                                                                            <  1:306269/145‑1 (MQ=255)                                                                           gatgatGTCTACCTGTACACCGTCGACGACCTGCACGATGTAGTGGCGGAAAACCTCAAGAGCCGCCAGGGCGCGGCCCAGGCTGCCGAAGAGCTGGTATCCGTGGGCGCCGAAGACTTCATGCTGCGCCTGCGCGAACGGGCTGCa                                                                            <  7:230492/147‑1 (MQ=255)                                                                           gatgatGTCTACCTGTACACCGTCGACGACCTGCACGATGTAGTGGCGGAAAACCTCAAGAGCCGCCAGGGCGCGGCCCAGGCGGCCGAAGAGCTGGTATCCGTGGGCGCCGAAGACTTCATGCTGCGCCTGCGCGAACTGGCTGCAGt                                                                          >  4:36840/1‑149 (MQ=255)                                                                              gatgTCTACCTGTACACCGTCGACGACCTGCACGATGTAGTGGCGGAAAACCTCAAGAGCCGCCAGGGCGCGGCCCAGGCGGCCGAAGAGCTGGTATCCGTGGGCGCCGAAGACTTCATGCTGCGCCTGCGCGAACTGGCTg                                                                              >  5:62770/1‑142 (MQ=255)                                                                              gatgTCTACCTGTACACCGTCGACGACCTGCACGATGTAGTGGCGGAAAACCTCAAGAGCCGCCAGGGCGCGGCCCAGGCGGCCGAAGAGCTGGTATCCGTGGGCGCCGAAGACTTCATGCTGCGCCTGCGCGAACTGGCTg                                                                              <  6:62770/142‑1 (MQ=255)                                                                                tgTCTACCTGTACACCGTCGACGACCTGCACGATGTAGTGGCGGAAAACCTCAAGAGCCGCCAGGGCGCGGCCCAGGCGGCCGAAGAGCTGGTATCCGTGGGCGCCGAAGACTTCATGCTGCGCCTGCGCGAACTGGCTGCAGTGGAc                                                                      <  6:63284/148‑1 (MQ=255)                                                                                    tACCTGTACACCGTCGACGACCCGCACGATGTAGTGGCGGAAAACCTCAAGAGCCGCCAGGGCGCGGCCCAGGCGGCCGAAGAGCTGGTATCCGTGGGCGCCGAAGACTTCATGCTGCGCCTGCGCGAACTGGCTGCAGTGGACGTGCt                                                                 >  2:96254/1‑149 (MQ=255)                                                                                          tACACCGTCGACGACCTGCACGATGTAGTGGCGGAAAACCTCAAGAGCCGCCAGGGCGCGGCCCAGGCGGCCGAAGAGCTGGTATCCGTGGGCGCCGAAGACTTCATGCTGCGCCTGCGCGAACTGGCTGCAGTGGACGTGCTCAgg                                                             <  4:55254/147‑1 (MQ=255)                                                                                                   gacgacCTGCACGATGTAGTGGCGGAAAACCTCAAGAGCCGCCAGGGCGCGGCCCAGGCGGCCGAAGAGCTGGTATCCGTGGGCGCCGAAGACTTCATGCTGCGCCTGCGCGAACTGGCTGCAg                                                                           >  1:226218/1‑124 (MQ=255)                                                                                                   gacgacCTGCACGATGTAGTGGCGGAAAACCTCAAGAGCCGCCAGGGCGCGGCCCAGGCGGCCGAAGAGCTGGTATCCGTGGGCGCCGAAGACTTCATGCTGCGCCTGCGCGAACTGGCTGCAg                                                                           <  2:226218/124‑1 (MQ=255)                                                                                                      gacCTGCACGATGTAGTGGCGGAAAACCTCAAGAGCCGCCAGGGCGCGGCCCAGGCGGCCGAAGAGCTGGTATCCGTGGGCGCCGAAGACTTCATGCTGCGCCTGCGCGAACTGGCTGCAGTGGACGTGCTCAGGGCCTATCGCCAGc                                                >  2:208417/1‑148 (MQ=255)                                                                                                         cTGCACGATGTAGTGGCGGAAAACCTCAAGAGCCGCCAGGGCGCGGCCCAGGCGGCCGAAGAGCTGGTATCCGTGGGCGCCGAAGACTTCATGCTGCGCCTGCGCGAACTGGCTGCAGTGGACGTGCTCAGGGCCTATCGCCAGCAAAg                                            >  1:313359/1‑149 (MQ=255)                                                                                                            cACGATGTAGTGGCGGAAAACCTCAAGAGCCGCCAGGGCGCGGCCCAGGCGGCCGAAGAGCTGGTATCCGTGGGCGCCGAAGACTTCATGCTGCGCCTGCGCGAACTGGCTGCAGTGGACGTGCTCAGGGCCTATCGCCAGCAAAGt                                           >  1:155407/1‑147 (MQ=255)                                                                                                             aCGATGTAGTGGCGGAAAACCTCAAGAGCCGCCAGGGCGCGGCCCAGGCGGCCGAAGAGCTGGTATCCGTGGGCGCCGAAGACTTCATGCTGCGCCTGCGCGAACTGGCTGCAGTGGACGTGCTCAGGGCCTATCGCCAGCAAAGTGAg                                        <  1:208417/149‑1 (MQ=255)                                                                                                               gATGTAGTGGCGGAAAACCTCAAGAGCCGCCAGGGCGCGGCCCAGGCGGCCGAAGAGCTGGTATCCGTGGGCGCCGAAGACTTCATGCTGCGCCTGCGCGAACTGGCTGCAGTGGACGTGCT‑AGGGCCTATCGCCAGCAAAGTGAgcgc                                     >  8:125305/1‑149 (MQ=255)                                                                                                                     gTGGCGGAAAACCTCAAGAGCCGCCAGGGCGCGGCCCAGGCGGCCGAAGAGCTGGTATCCGTGGGCGCCGAAGACTTCATGCTGCGCCTGCGCGAACTGGCTGCAGTGGACGTGCTCAGGGCCTATCGCCAGCAAAGTGAGCGCCTTcg                                <  1:3196/149‑1 (MQ=255)                                                                                                                        gCGGAAAACCTCAAGAGCCGCCAGGGCGCGGCCCAGGCGGCCGAAGAGCTGGTATCCGTGGGCGCCGAAGACTTCATGCTGCGCCTGCGCGAACTGGCTGCAGTGGACGTGCTCAGGGCCTATCGCCAGCAAAGTGAGCGCCTTCGcga                             >  5:103845/1‑149 (MQ=255)                                                                                                                        gCGGAAAACCTCAAGAGCCGCCAGGGCGCGGCCCAGGCGGCCGAAGAGCTGGTATCCGTGGGCGCCGAAGACTTCATGCTGCGCCTGCGCGAACTGGCTGCAGTGGACGTGCTCAGGGCCTATCGCCAGCAAAGTGAGCGCCTTCGcga                             <  2:155407/149‑1 (MQ=255)                                                                                                                          ggAAAACCTCAAGAGCCGCCAGGGCGCGGCCCAGGCGGCCGAAGAGCTGGTATCCGTGGGCGCCGAAGACTTCATGCTGCGCCTGCGCGAACTGGCTGCAGTGGACGTGCTCAggg                                                            <  6:282260/116‑1 (MQ=255)                                                                                                                          ggAAAACCTCAAGAGCCGCCAGGGCGCGGCCCAGGCGGCCGAAGAGCTGGTATCCGTGGGCGCCGAAGACTTCATGCTGCGCCTGCGCGAACTGGCTGCAGTGGACGTGCTCAggg                                                            >  5:282260/1‑116 (MQ=255)                                                                                                                            aaaaCCTCAAGAGCCGCCAGGGCGCGGCCCAGGCGGCCGAAGAGCTGGTATCCGTGGGCGCCGAAGACTTCATGCTGCGCCTGCGCGAACTGGCTGCAGTGGACGTGCTCAGGGCCTATCGCCAGCAAAGTGAGCGCCTTCGCGACgaa                         <  3:36840/149‑1 (MQ=255)                                                                                                                             aaaCCTCAAGAGCCGCCAGGGCGCGGCCCAGGCGGCCGAAGAGCTGGTATCCGTGGGCGCCGAAGACTTCATGCTGCGCCTGCGCGAACTGGCTGCAGTGGACGTGCTCAGGGCCTATCGCCAGCAAAGTGAGCGCCTTCGCGACgaag                        <  6:103845/149‑1 (MQ=255)                                                                                                                                  tCAAGAGCCGCCAGGGCGCGGCCCAGGCGGCCGAAGAGCTGGTATCCGTGGGCGCCGAAGACTTCATGCTGCGCCTGCGCGAACTGGCTGCAGTGGACGTGCTCa                                                               <  7:173783/105‑1 (MQ=255)                                                                                                                                  tCAAGAGCCGCCAGGGCGCGGCCCAGGCGGCCGAAGAGCTGGTATCCGTGGGCGCCGAAGACTTCATGCTGCGCCTGCGCGAACTGGCTGCAGTGGACGTGCTCa                                                               >  8:173783/1‑105 (MQ=255)                                                                                                                                      gagCCGCCAGGGCGCGGCCCAGGCGGCCGAAGAGCTGGTATCCGTGGGCGCCGAAGACTTCATGCTGCGCCTGCGCGAACTGGCTGCAGTGGACGTGCT‑AGGGCCTATCGCCAGCAAAGTGAGCGCCTTCGCGACGAAGAACTGCaaaa              <  7:125305/149‑1 (MQ=255)                                                                                                                                         ccgccTGGGCCGCGCCCTGGCGGCCGAAGAGCTGGTATCCGTGGGCGCCGAAGACTTCATGCTGCGCCTGCGCGAACTGGCTGCAGTGGACGTGCTCAGGGCCTATCGCCAGCAAAGTGAGCGCCTTCGcgac                            <  7:62327/133‑1 (MQ=255)                                                                                                                                         ccgccTGGGCCGCGCCCTGGCGGCCGAAGAGCTGGTATCCGTGGGCGCCGAAGACTTCATGCTGCGCCTGCGCGAACTGGCTGCAGTGGACGTGCTCAGGGCCTATCGCCAGCAAAGTGAGCGCCTTCGcgac                            >  8:62327/1‑133 (MQ=255)                                                                                                                                                ggCGCGGCCCAGGCGGCCGAAGAGCTGGTATCCGTGGGCGCCGAAGACTTCATGCTGCGCCTGCGCGAACTGGCTGCAGTGGACGTGCTCAGGGCCTATCGCCAGCAAAGTGAGCGCCTTCGCGACGAAGAACTGCAAAAGGCCTTgcg     >  4:259716/1‑149 (MQ=255)                                                                                                                                                 gcgcGGCCCAGGCGGCCGAAGAGCTGGTATCCGTGGGCGCCGAAGACTTCATGCTGCGCCTGCGCGAACTGGCTGCAGTGGACGTGCTCAGGGCCTATCGCCAGCAAAGTGAGCGCCTTCGCGACGAAGAACTGCAAAAGGCCTTgcgc    <  2:253305/149‑1 (MQ=255)                                                                                                                                                   gcgGCCCAGGCGGCCGAAGAGCTGGTATCCGTGGGCGCCGAAGACTTCATGCTGCGCCTGCGCGAACTGGCTGCAGTGGACGTGCTCAGGGCCTATCGCCAGCAAAGTGAGCGCCTTCGCGACGAAGAACTGCAAAAGGCCTTGCGCct  >  6:319005/1‑149 (MQ=255)                                                                                                                                                     |                                                                                                                                                   AGCGCCGACACAAGCCCATCTTCATGGTCGACATCGCTGTACCTCGCGATATCGAAACCGAAGTCGGCGAGCTGGATGATGTCTACCTGTACACCGTCGACGACCTGCACGATGTAGTGGCGGAAAACCTCAAGAGCCGCCAGGGCGCGGCCCAGGCGGCCGAAGAGCTGGTATCCGTGGGCGCCGAAGACTTCATGCTGCGCCTGCGCGAACTGGCTGCAGTGGACGTGCTCAGGGCCTATCGCCAGCAAAGTGAGCGCCTTCGCGACGAAGAACTGCAAAAGGCCTTGCGCCT  >  NC_002947/852018‑852312

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑