Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 852,171 A→T 7.2% Q317L (CAG→CTG)  hemA → glutamyl‑tRNA reductase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_002947852,1710AT 7.2% 165.4 / 2.3 55Q317L (CAG→CTG) hemAglutamyl‑tRNA reductase
Reads supporting (aligned to +/- strand):  ref base A (24/27);  new base T (2/2);  total (26/29)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.97e-01

CCATCTTCATGGTCGACATCGCTGTACCTCGCGATATCGAAACCGAAGTCGGCGAGCTGGATGATGTCTACCTGTACACCGTCGACGACCTGCACGATGTAGTGGCGGAAAACCTCAAGAGCCGCCAGGGCGCGGCCCAGGCGGCCGAAGAGCTGGTATCCGTGGGCGCCGAAGACTTCATGCTGCGCCTGCGCGAACTGGCTGCAGTGGACGTGCTCAGGGCCTATCGCCAGCAAAGTGAGCGCCTTCGCGACGAAGAACTGCAAAAGGCCTTGCGCCTGCT  >  NC_002947/852033‑852315
                                                                                                                                          |                                                                                                                                                
ccATCTTCATGGTCGACATCGCTGTACCTCGCGATATCGAAACCGAAGTCGGCGAGCTGGATGATGTCTACCTGTACACCGTCGACGACCTGCACGATGTAGTGGCGGAAAACCTCAAGAGCCGCCAGGGCGCGGCCCAGGCGGCCGaa                                                                                                                                        <  1:115555/149‑1 (MQ=255)
ccATCTTCATGGTCGACATCGCTGTACCTCGCGATATCGAAACCGAAGTCGGCGAGCTGGATGATGTCTACCTGTACACCGTCGACGACCTGCACGATGTAGTGGCGGAAAACCTCAAGAGCCGCCAGGGCGCGGCCCAGGCGGCCGaa                                                                                                                                        <  3:327438/149‑1 (MQ=255)
 cATCTTCATGGTCGACATCGCTGTACCTCGCGATATCGAAACCGAAGTCGGCGAGCTGGATGATGTCTACCTGTACACCGTCGACGACCTGCACGATGTAGTGGCGGAAAACCTCAAGAGCCGCCAGGGCGCGGCCCAGGCGGCCg                                                                                                                                          >  5:89262/1‑146 (MQ=255)
 cATCTTCATGGTCGACATCGCTGTACCTCGCGATATCGAAACCGAAGTCGGCGAGCTGGATGATGTCTACCTGTACACCGTCGACGACCTGCACGATGTAGTGGCGGAAAACCTCAAGAGCCGCCAGGGCGCGGCCCAGGCGGCCGaa                                                                                                                                        <  6:89262/148‑1 (MQ=255)
  aTCTTCATGGTCGACATCGCTGTACCTCGCGATATCGAAACCGAAGTCGGCGAGCTGGATGATGTCTACCTGTACACCGTCGACGACCTGCACGATGTAGTGGCGGAAAACCTCAAGAGCCGCCAGGGCGCGGCCCAGGCGGCCGaa                                                                                                                                        >  2:328285/1‑147 (MQ=255)
       cATGGTCGACATCGCTGTACCTCGCGATATCGAAACCGAAGTCGGCGAGCTGGATGATGTCTACCTGTACACCGTCGACGACCTGCACGATGTAGTGGCGGAAAACCTCAAGAGCCGCCAGGGCGCGGCCCAGGCGGCCGAAGAGCTg                                                                                                                                  <  7:276118/148‑1 (MQ=255)
       cATGGTCGACATCGCTGTACCTCGCGATATCGAAACCGAAGTCGGCGAGCTGGATGATGTCTACCTGTACACCGTCGACGACCTGCACGATGTAGTGGCGGAAAACCTCAAGAGCCGCCAGGGCGCGGCCCAGGCGGCCGAAGAGCTg                                                                                                                                  <  1:328285/148‑1 (MQ=255)
       cATGGTCGACATCGCTGTACCTCGCGATATCGAAACCGAAGTCGGCGAGCTGGATGATGTCTACCTGTACACCGTCGACGACCTGCACGATGTAGTGGCGGAAAACCTCAAGAGCCGCCAGGGCGCGGCCCAGGCGGCCGAAGAGCTg                                                                                                                                  <  8:331868/148‑1 (MQ=255)
        aTGGTCGACATCGCTGTACCTCGCGATATCGAAACCGAAGTCGGCGAGCTGGATGATGTCTACCTGTACACCGTCGACGACCTGCACGATGTAGTGGCGGAAAACCTCAAGAGCCGCCTGGGCCGCGCCCTggcggc                                                                                                                                            <  2:248916/137‑1 (MQ=255)
        aTGGTCGACATCGCTGTACCTCGCGATATCGAAACCGAAGTCGGCCAGCTGGATGATGTCTACCTGTACACCGTCGACGACCTGCACGATGTAGTGGCGGAAAACCTCAAGAGCCGCCTGGGCCGCGCCCTggcggc                                                                                                                                            >  1:248916/1‑137 (MQ=255)
               aCATCGCTGTACCTCGCGATATCGAAACCGAAGTCGGCGAGCTGGATGATGTCTACCTGTACACCGTCGACGACCTGCACGATGTAGTGGCGGAAAACCTCAAGAGCCGCCAGGGCGCGGCCCAGGCGGCCGAAGATCTGGTATACGTg                                                                                                                         >  3:221603/1‑149 (MQ=255)
               aCATCGCTGTACCTCGCGATATCGAAACCGAAGTCGGCGAGCTGGATGATGTCTACCTGTACACCGTCGACGACCTGCACGATGTAGTGGCGGAAAACCTCAAGAGCCGCCAGGGCGCGGCCCAGGCGGCCGAAGAGCTGGTATCCGTg                                                                                                                         >  5:17077/1‑149 (MQ=255)
                     cTGTACCTCGCGATATCGAAACCGAAGTCGGCGAGCTGGATGATGTCTACCTGTACACCGTCGACGACCTGCACGATGTAGTGGCGGAAAACCTCAAGAGCCGCCAGGGCGCGGCCCAGGCGGCCGAAGAGCTGGTATCCGTGGGCGcc                                                                                                                   >  5:63284/1‑149 (MQ=255)
                       gTACCTCGCGATATCGAAACCGAAGTCGGCGAGCTGGATGATGTCTACCTGTACACCGTCGACGACCTGCACGATGTAGTGGCGGAAAACCTCAAGAGCCGCCAGGGCGCGGCCCAGGCGGCCGAAGAGCTGGTATCCGTGGGCGCCg                                                                                                                  >  2:3196/1‑148 (MQ=255)
                                 atgtCGAAACCGAAGTCGGCGAGCTGGATGATGTCTACCTGTACACCGTCGACGACCTGCACGATGTAGTGGCGGAAAACCTCAAGAGCCGCCAGGGCGCGGCCCAGGCGGCCGAAGAGCTGGTATCCGTGGGCGCCGAAGACTTCAtg                                                                                                       <  4:230484/146‑1 (MQ=255)
                                  tatCGAAACCGAAGTCGGCGAGCTGGATGATGTCTACCTGTACACCGTCGACGACCTGCACGATGTAGTGGCGGAAAACCTCAAGAGCCGCCAGGGCGCGGCCCAGGCGGcc                                                                                                                                           <  8:308120/112‑1 (MQ=255)
                                  tatCGAAACCGAAGTCGGCGAGCTGGATGATGTCTACCTGTACACCGTCGACGACCTGCACGATGTAGTGGCGGAAAACCTCAAGAGCCGCCAGGGCGCGGCCCAGGCGGcc                                                                                                                                           >  7:308120/1‑112 (MQ=255)
                                    tCGAAACCGAAGTCGGCGAGCTGGATGATGTCTACCTGTACACCGTCGACGACCTGCACGATGTAGTGGCGGAAAACCTCAAGAGCCGCCAGGGCGCGGCCCAGGCGGCCGAAGAGCTGGTATCCGTGGGCGCCGAAGACTTCAtgctg                                                                                                    >  3:55254/1‑149 (MQ=255)
                                      gAAACAGAAGTCGGCGAGCTGGGTGATGTCTACCTGTACACCGTCGACGACCTGCACGATGTAGTGGCGGAAAACCTCAAGAGCCGCCAGGGCGCGGCCCAGGCGGCCGAAGAGCTGGTATCCGTGGGCGCCGAAGACTTCATGctgcg                                                                                                  >  2:306269/1‑149 (MQ=255)
                                         aCCGAAGTCGGCGAGCTGGATGATGTCTACCTGTACACCGTCGACGACCTGCACGATGTAGTGGCGGAAAACCTCAAGAGCCGCCAGGGCGCGGCCCAGGCGGCCGAAGAGCTGGTATCCGTGGGCGCCGAAGACTTCa                                                                                                         <  5:82796/139‑1 (MQ=255)
                                         aCCGAAGTCGGCGAGCTGGATGATGTCTACCTGTACACCGTCGACGACCTGCACGATGTAGTGGCGGAAAACCTCAAGAGCCGCCAGGGCGCGGCCCAGGCGGCCGAAGAGCTGGTATCCGTGGGCGCCGAAGACTTCa                                                                                                         >  6:82796/1‑139 (MQ=255)
                                                    cGAGCTGGATGATGTCACCCTGTACCCCGTCGACGACCTCCACGATGTAGTGGCGTAAAACCTCAAGCGCCGCCAGGGCGCGGCCCAGGCGGCCTAAGAGCTGGTATCCGTGGGCGCCGAAGACTTCATGCTGCGCCTGCGCGAACTgg                                                                                    <  7:292168/149‑1 (MQ=255)
                                                     gAGCTGGATGATGTCTACCTGTACACCGTCGACGACCTGCACGATGTAGTGGCGGAAAACCTCAAGAGCCGCCAGGGCGCGGCCCAGGCGGCCGAAGAGCTGGTATCCGTGGGCGCCGAAGACTTCATGCTGCGCCTGCGCGAACTGGc                                                                                   <  6:17077/149‑1 (MQ=255)
                                                       gCTGGATGATGTCTACCTGTACACCGTCGACGACCTGCACGATGTAGTGGCGGAAAACCTCAAGAGCCGCCAGGGCGCGGCCCAGGCGGCCGAAGAGCTGGTATCCGTGGGCGCCGAAGACTTCATGCTGCGCCTGCGCGAACTGGCTg                                                                                 >  5:146689/1‑149 (MQ=255)
                                                           ggtgatgTCTACCTGTACACCGTCGACGAACTGCACGATGTAGTGGCGGAAAACCTCAAGAGCCGCCAGGGCGCGGCCCAGGCGGCCGAAGAGCTGGTATCCGTGGGCGCCGAAGACTTCATGCTGCGCCTGCGCGAACTGGCTGCa                                                                               <  1:306269/145‑1 (MQ=255)
                                                           gatgatGTCTACCTGTACACCGTCGACGACCTGCACGATGTAGTGGCGGAAAACCTCAAGAGCCGCCAGGGCGCGGCCCAGGCTGCCGAAGAGCTGGTATCCGTGGGCGCCGAAGACTTCATGCTGCGCCTGCGCGAACGGGCTGCa                                                                               <  7:230492/147‑1 (MQ=255)
                                                           gatgatGTCTACCTGTACACCGTCGACGACCTGCACGATGTAGTGGCGGAAAACCTCAAGAGCCGCCAGGGCGCGGCCCAGGCGGCCGAAGAGCTGGTATCCGTGGGCGCCGAAGACTTCATGCTGCGCCTGCGCGAACTGGCTGCAGt                                                                             >  4:36840/1‑149 (MQ=255)
                                                              gatgTCTACCTGTACACCGTCGACGACCTGCACGATGTAGTGGCGGAAAACCTCAAGAGCCGCCAGGGCGCGGCCCAGGCGGCCGAAGAGCTGGTATCCGTGGGCGCCGAAGACTTCATGCTGCGCCTGCGCGAACTGGCTg                                                                                 >  5:62770/1‑142 (MQ=255)
                                                              gatgTCTACCTGTACACCGTCGACGACCTGCACGATGTAGTGGCGGAAAACCTCAAGAGCCGCCAGGGCGCGGCCCAGGCGGCCGAAGAGCTGGTATCCGTGGGCGCCGAAGACTTCATGCTGCGCCTGCGCGAACTGGCTg                                                                                 <  6:62770/142‑1 (MQ=255)
                                                                tgTCTACCTGTACACCGTCGACGACCTGCACGATGTAGTGGCGGAAAACCTCAAGAGCCGCCAGGGCGCGGCCCAGGCGGCCGAAGAGCTGGTATCCGTGGGCGCCGAAGACTTCATGCTGCGCCTGCGCGAACTGGCTGCAGTGGAc                                                                         <  6:63284/148‑1 (MQ=255)
                                                                    tACCTGTACACCGTCGACGACCCGCACGATGTAGTGGCGGAAAACCTCAAGAGCCGCCAGGGCGCGGCCCAGGCGGCCGAAGAGCTGGTATCCGTGGGCGCCGAAGACTTCATGCTGCGCCTGCGCGAACTGGCTGCAGTGGACGTGCt                                                                    >  2:96254/1‑149 (MQ=255)
                                                                          tACACCGTCGACGACCTGCACGATGTAGTGGCGGAAAACCTCAAGAGCCGCCAGGGCGCGGCCCAGGCGGCCGAAGAGCTGGTATCCGTGGGCGCCGAAGACTTCATGCTGCGCCTGCGCGAACTGGCTGCAGTGGACGTGCTCAgg                                                                <  4:55254/147‑1 (MQ=255)
                                                                                   gacgacCTGCACGATGTAGTGGCGGAAAACCTCAAGAGCCGCCAGGGCGCGGCCCAGGCGGCCGAAGAGCTGGTATCCGTGGGCGCCGAAGACTTCATGCTGCGCCTGCGCGAACTGGCTGCAg                                                                              <  2:226218/124‑1 (MQ=255)
                                                                                   gacgacCTGCACGATGTAGTGGCGGAAAACCTCAAGAGCCGCCAGGGCGCGGCCCAGGCGGCCGAAGAGCTGGTATCCGTGGGCGCCGAAGACTTCATGCTGCGCCTGCGCGAACTGGCTGCAg                                                                              >  1:226218/1‑124 (MQ=255)
                                                                                      gacCTGCACGATGTAGTGGCGGAAAACCTCAAGAGCCGCCAGGGCGCGGCCCAGGCGGCCGAAGAGCTGGTATCCGTGGGCGCCGAAGACTTCATGCTGCGCCTGCGCGAACTGGCTGCAGTGGACGTGCTCAGGGCCTATCGCCAGc                                                   >  2:208417/1‑148 (MQ=255)
                                                                                         cTGCACGATGTAGTGGCGGAAAACCTCAAGAGCCGCCAGGGCGCGGCCCAGGCGGCCGAAGAGCTGGTATCCGTGGGCGCCGAAGACTTCATGCTGCGCCTGCGCGAACTGGCTGCAGTGGACGTGCTCAGGGCCTATCGCCAGCAAAg                                               >  1:313359/1‑149 (MQ=255)
                                                                                            cACGATGTAGTGGCGGAAAACCTCAAGAGCCGCCAGGGCGCGGCCCAGGCGGCCGAAGAGCTGGTATCCGTGGGCGCCGAAGACTTCATGCTGCGCCTGCGCGAACTGGCTGCAGTGGACGTGCTCAGGGCCTATCGCCAGCAAAGt                                              >  1:155407/1‑147 (MQ=255)
                                                                                             aCGATGTAGTGGCGGAAAACCTCAAGAGCCGCCAGGGCGCGGCCCAGGCGGCCGAAGAGCTGGTATCCGTGGGCGCCGAAGACTTCATGCTGCGCCTGCGCGAACTGGCTGCAGTGGACGTGCTCAGGGCCTATCGCCAGCAAAGTGAg                                           <  1:208417/149‑1 (MQ=255)
                                                                                               gATGTAGTGGCGGAAAACCTCAAGAGCCGCCAGGGCGCGGCCCAGGCGGCCGAAGAGCTGGTATCCGTGGGCGCCGAAGACTTCATGCTGCGCCTGCGCGAACTGGCTGCAGTGGACGTGCT‑AGGGCCTATCGCCAGCAAAGTGAgcgc                                        >  8:125305/1‑149 (MQ=255)
                                                                                                     gTGGCGGAAAACCTCAAGAGCCGCCAGGGCGCGGCCCAGGCGGCCGAAGAGCTGGTATCCGTGGGCGCCGAAGACTTCATGCTGCGCCTGCGCGAACTGGCTGCAGTGGACGTGCTCAGGGCCTATCGCCAGCAAAGTGAGCGCCTTcg                                   <  1:3196/149‑1 (MQ=255)
                                                                                                        gCGGAAAACCTCAAGAGCCGCCAGGGCGCGGCCCAGGCGGCCGAAGAGCTGGTATCCGTGGGCGCCGAAGACTTCATGCTGCGCCTGCGCGAACTGGCTGCAGTGGACGTGCTCAGGGCCTATCGCCAGCAAAGTGAGCGCCTTCGcga                                >  5:103845/1‑149 (MQ=255)
                                                                                                        gCGGAAAACCTCAAGAGCCGCCAGGGCGCGGCCCAGGCGGCCGAAGAGCTGGTATCCGTGGGCGCCGAAGACTTCATGCTGCGCCTGCGCGAACTGGCTGCAGTGGACGTGCTCAGGGCCTATCGCCAGCAAAGTGAGCGCCTTCGcga                                <  2:155407/149‑1 (MQ=255)
                                                                                                          ggAAAACCTCAAGAGCCGCCAGGGCGCGGCCCAGGCGGCCGAAGAGCTGGTATCCGTGGGCGCCGAAGACTTCATGCTGCGCCTGCGCGAACTGGCTGCAGTGGACGTGCTCAggg                                                               <  6:282260/116‑1 (MQ=255)
                                                                                                          ggAAAACCTCAAGAGCCGCCAGGGCGCGGCCCAGGCGGCCGAAGAGCTGGTATCCGTGGGCGCCGAAGACTTCATGCTGCGCCTGCGCGAACTGGCTGCAGTGGACGTGCTCAggg                                                               >  5:282260/1‑116 (MQ=255)
                                                                                                            aaaaCCTCAAGAGCCGCCAGGGCGCGGCCCAGGCGGCCGAAGAGCTGGTATCCGTGGGCGCCGAAGACTTCATGCTGCGCCTGCGCGAACTGGCTGCAGTGGACGTGCTCAGGGCCTATCGCCAGCAAAGTGAGCGCCTTCGCGACgaa                            <  3:36840/149‑1 (MQ=255)
                                                                                                             aaaCCTCAAGAGCCGCCAGGGCGCGGCCCAGGCGGCCGAAGAGCTGGTATCCGTGGGCGCCGAAGACTTCATGCTGCGCCTGCGCGAACTGGCTGCAGTGGACGTGCTCAGGGCCTATCGCCAGCAAAGTGAGCGCCTTCGCGACgaag                           <  6:103845/149‑1 (MQ=255)
                                                                                                                  tCAAGAGCCGCCAGGGCGCGGCCCAGGCGGCCGAAGAGCTGGTATCCGTGGGCGCCGAAGACTTCATGCTGCGCCTGCGCGAACTGGCTGCAGTGGACGTGCTCa                                                                  <  7:173783/105‑1 (MQ=255)
                                                                                                                  tCAAGAGCCGCCAGGGCGCGGCCCAGGCGGCCGAAGAGCTGGTATCCGTGGGCGCCGAAGACTTCATGCTGCGCCTGCGCGAACTGGCTGCAGTGGACGTGCTCa                                                                  >  8:173783/1‑105 (MQ=255)
                                                                                                                      gagCCGCCAGGGCGCGGCCCAGGCGGCCGAAGAGCTGGTATCCGTGGGCGCCGAAGACTTCATGCTGCGCCTGCGCGAACTGGCTGCAGTGGACGTGCT‑AGGGCCTATCGCCAGCAAAGTGAGCGCCTTCGCGACGAAGAACTGCaaaa                 <  7:125305/149‑1 (MQ=255)
                                                                                                                         ccgccTGGGCCGCGCCCTGGCGGCCGAAGAGCTGGTATCCGTGGGCGCCGAAGACTTCATGCTGCGCCTGCGCGAACTGGCTGCAGTGGACGTGCTCAGGGCCTATCGCCAGCAAAGTGAGCGCCTTCGcgac                               <  7:62327/133‑1 (MQ=255)
                                                                                                                         ccgccTGGGCCGCGCCCTGGCGGCCGAAGAGCTGGTATCCGTGGGCGCCGAAGACTTCATGCTGCGCCTGCGCGAACTGGCTGCAGTGGACGTGCTCAGGGCCTATCGCCAGCAAAGTGAGCGCCTTCGcgac                               >  8:62327/1‑133 (MQ=255)
                                                                                                                                ggCGCGGCCCAGGCGGCCGAAGAGCTGGTATCCGTGGGCGCCGAAGACTTCATGCTGCGCCTGCGCGAACTGGCTGCAGTGGACGTGCTCAGGGCCTATCGCCAGCAAAGTGAGCGCCTTCGCGACGAAGAACTGCAAAAGGCCTTgcg        >  4:259716/1‑149 (MQ=255)
                                                                                                                                 gcgcGGCCCAGGCGGCCGAAGAGCTGGTATCCGTGGGCGCCGAAGACTTCATGCTGCGCCTGCGCGAACTGGCTGCAGTGGACGTGCTCAGGGCCTATCGCCAGCAAAGTGAGCGCCTTCGCGACGAAGAACTGCAAAAGGCCTTgcgc       <  2:253305/149‑1 (MQ=255)
                                                                                                                                   gcgGCCCAGGCGGCCGAAGAGCTGGTATCCGTGGGCGCCGAAGACTTCATGCTGCGCCTGCGCGAACTGGCTGCAGTGGACGTGCTCAGGGCCTATCGCCAGCAAAGTGAGCGCCTTCGCGACGAAGAACTGCAAAAGGCCTTGCGCct     >  6:319005/1‑149 (MQ=255)
                                                                                                                                      gCCCAGGCGGCCGAAGAGCTGGTATCCGTGGGCGCCGAAGACTTCATGCTGCGCCTGCGCGAACTGGCTGCAGTGGACGTGCTCAGGGCCTATCGCCAGCAAAGTGAGCGCCTTCGCGACGAAGAACTGCAAAAGGCCTTGCGCctgct  <  2:313359/149‑1 (MQ=255)
                                                                                                                                          |                                                                                                                                                
CCATCTTCATGGTCGACATCGCTGTACCTCGCGATATCGAAACCGAAGTCGGCGAGCTGGATGATGTCTACCTGTACACCGTCGACGACCTGCACGATGTAGTGGCGGAAAACCTCAAGAGCCGCCAGGGCGCGGCCCAGGCGGCCGAAGAGCTGGTATCCGTGGGCGCCGAAGACTTCATGCTGCGCCTGCGCGAACTGGCTGCAGTGGACGTGCTCAGGGCCTATCGCCAGCAAAGTGAGCGCCTTCGCGACGAAGAACTGCAAAAGGCCTTGCGCCTGCT  >  NC_002947/852033‑852315

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑