Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 875,285 G→T 7.4% intergenic (+24/+22) PP_0759 → / ← PP_0760 hypothetical protein/membrane protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_002947875,2850GT 7.4% 210.2 / 4.8 66intergenic (+24/+22)PP_0759/PP_0760hypothetical protein/membrane protein
Reads supporting (aligned to +/- strand):  ref base G (30/31);  new base T (3/2);  total (33/33)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.88e-01

GCTTCCGAAGGTGCACCGCCTGAGTTCATGAGTACTCACCCGGCGTCCGCGAGCCGCATTGCTTCGCTGCAGGCGGCGATTCCGAAGGTGATGCCGCTGTATCAGGCTGCCGCCAAG‑CCGTAAATGCCTGAATCCTGGGGCTGCATGGCAGCCC‑CCCCGGTTTTCCCTTTACCCAATCCAGCCACTGGCCTTCATTGCCGAATAGACGGCCACGATTGCCAGTACGAAGAACGCCGTTGCCGCCAGGCGGCGAATCAGCGTCAACGGCAGTTTGTCTGCCGCGAAGTTACCC  >  NC_002947/875139‑875430
                                                                                                                                                   |                                                                                                                                                  
gCTTCCGAAGGTGCACCGCCTGAGTTCATGAGTACTCACCCGGCGTCCGCGAGCCGCATTGCTTCGCTGCAGGCGGCGATTCCGAAGGTGATGCCGCTGTATCAGGCTGCCGCCAAG‑CCGTAAATGCCTGAATCCTGGGGCTGCATgg                                                                                                                                                   >  2:79321/1‑148 (MQ=255)
          gTGCACCGCCTGAGTTCATGAGTACTCACCCGGCGTCCGCGAGCCGCATTGCTTCGCTGCAGGCGGCGATTCCGAAGGTGATGCCGCTGTATCAGGCTGCCGCCAAG‑CCGTAAATGCCTGAATCCTGGGGCTGCCATGCAGccc‑cagga                                                                                                                                       <  3:218416/149‑5 (MQ=255)
          gTGCACCGCCTGAGTTCATGAGTACTCACCCGGCGTCCGCGAGCCGCATTGCTTCGCTGCAGGCGGCGATTCCGAAGGTGATGCCGCTGTATCAGGCTGCCGCCAAG‑CCGTAAATGCCTGAATCCGGGGGCTGCCATGCAGccc‑cagga                                                                                                                                       <  7:191952/149‑5 (MQ=255)
            gCACCGCCTGAGTTCATGAGTACTCACCCGGCGTCCGCGAGCCGCATTGCTTCGCTGCAGGCGGCGATTCCGAAGGTGATGCCGCTGTATCAGGCTGCCGCCAAG‑CCGTAAATGCCTGAATCCTGGGGCTGCATGGCAGCCC‑CCCCGGt                                                                                                                                     >  2:270417/1‑149 (MQ=255)
               ccGCCTGAGTTCATGAGTACTCACCCGGCGTCCGCGAGCCGCATTGCTTCGCTGCAGGCGGCGATTCCGAAGGTGATGCCGCTGTATCAGGCTGCCGCCAAG‑CCGTAAATGCCTGAATCCTGGGGCTGCATGGCAGCCC‑CCCCGGtttt                                                                                                                                  <  3:271466/149‑1 (MQ=255)
               ccGCCTGAGTTCATGAGTACTCACCCGGCGTCCGCGAGCCGCATTGCTTCGCTGCAGGCGGCGATTCCGAAGGTGATGCCGCTGTATCAGGCTGCCGCCAAG‑CCGTAAATGCCTGAATCCTGGGGCTGCATGGCAGCCC‑CCCCGGtttt                                                                                                                                  >  7:31719/1‑149 (MQ=255)
                  ccTGAGTTAATGAGTACTCACCCGGCGTCCGCGAGCCGCATTGCTTCGCTGCAGGCGGCGATTCCGAAGGTGATGCCGCTGTATCAGGCTGCCGCCAAG‑CCGTAAATGCCTGAATCCTGGGGCTGCATGGCAGCCC‑CCCCGGTTTTccc                                                                                                                               >  6:214218/1‑149 (MQ=255)
                        ttCATGAGTACTCACCCGGCGTCCGCGAGCCGCATTGCTTCGCTGCAGGCGGCGATTCCGAAGGTGATGCCGCTGTATCAGGCTGCCGCCAAGCCCGTAAATGCCTGAATCCTGGGGCTGCATGGCAGCCC‑CCCCGGTTTTCCCTTTAc                                                                                                                          <  1:270417/149‑1 (MQ=255)
                        ttCATGAGTACTCACCCGGCGTCCGCGAGCCGCATTGCTTCGCTGCAGGCGGCGATTCCGAAGGTGATGCCGCTGTATCAGGCTGCCGCCAAG‑CCGTAAATGCCTGAATCCTGGGGCTGCATGGCAGCCC‑CCCCGGTTTTCCCTTTAcc                                                                                                                         >  3:6208/1‑149 (MQ=255)
                            tGAGTACTCACCCGGCGTCCGCGAGCCGCATTGCTTCGCTGCAGGCGGCGATTCCGAAGGTGATGCCGCTGTATCAGGCTGCCGCCAAG‑CCGTAAATGCCTGAATCCTGGGGCTGCATGGCAGCCC‑CCCCGGTTTTCCCTTTACCCAAt                                                                                                                     >  1:210005/1‑149 (MQ=255)
                            tGAGTACTCACCCGGCGTCCGCGAGCCGCATTGCTTCGCTGCAGGCGGCGATTCCGAAGGTGATGCCGCTGTATCAGGCTGCCGCCAAG‑CCGTAAATGCCTGAATCCTGGGGCTGCATGGCAGCCC‑CCCCGGTTTTCCCTTTACCCAAt                                                                                                                     >  7:103895/1‑149 (MQ=255)
                              aGTACTCACCCGGCGTCCGCGAGCCGCATTGCTTCGCTGCAGGCGGCGATTCCGAAGGTGATGCCGCTGTATCAGGCTGCCGCCAAG‑CCGTAAATGCCTGAATCCTGGGGCTGCATGGCAGCCC‑CCCCGGTTTTCCCTTTACCCAATc                                                                                                                    <  5:214218/148‑1 (MQ=255)
                              aGTACTCACCCGGCGTCCGCGAGCCGCATTGCTTCGCTGCAGGCGGCGATTCCGAAGGTGATGCCGCTGTATCAGGCTGCCGCCAAG‑CCGTAAATGCCTGAATCCTGGGGCTGCATGGCAGCCC‑CCCCGGTTTTCCCTTTACCCAATc                                                                                                                    <  8:103895/148‑1 (MQ=255)
                               gTACTCACCCGGCGTCCGCGAGCCGCATTGCTTCGCTGCAGGCGGCGATTCCGAAGGTGATGCCGCTGTATCAGGCTGCCGCCAAG‑CCGTAAATGCCTGAATCCTGGGGCTGCATGGCAGCCC‑CCCCGGTTTTc                                                                                                                                 >  4:307793/1‑134 (MQ=255)
                               gTACTCACCCGGCGTCCGCGAGCCGCATTGCTTCGCTGCAGGCGGCGATTCCGAAGGTGATGCCGCTGTATCAGGCTGCCGCCAAG‑CCGTAAATGCCTGAATCCTGGGGCTGCATGGCAGCCC‑CCCCGGTTTTc                                                                                                                                 <  3:307793/134‑1 (MQ=255)
                               gTACTCACCCGGCGTCCGCGAGCCGCATTGCTTCGCTGCAGGCGGCGATTCCGAAGGTGATGCCGCTGTATCAGGCTGCCGCCAAG‑CCGTAAATGCCTGAATCCTGGGGCTGCATGGCAGCCC‑CCCCGGTTTTCCCTTTACCCAATCCa                                                                                                                  <  8:257630/149‑1 (MQ=255)
                                  cTCACCCGGCGTCCGCGAGCCGCATTGCTTCGCTGCAGGCGGCGATTCCGAAGGTGATGCCGCTGTATCAGGCTGCCGCCAAG‑CCGTAAATGCCTGAATCCTGGGGCTGCATGGCAGCCC‑CCCCGGTTTTCCCTTTACCCAATCCAGc                                                                                                                <  5:251050/148‑1 (MQ=255)
                                  cTCACCCGGCGTCCGCGAGCCGCATTGCTTCGCTGCAGGCGGCGATTCCGAAGGTGATGCCGCTGTATCAGGCTGCCGCCAAG‑CCGTAAATGCCTGAATCCTGGGGCTGCACGGCAGCCC‑CCCGGGTTTTCCCTTTACCCAATCCAGcc                                                                                                               <  5:277628/149‑1 (MQ=255)
                                     aCCCGGCGTCCGCGAGCCGCATTGCTTCGCTGCAGGCGGCGATTCCGAAGGTGATGCCGCTGTATCAGGCTGCCGCCAAG‑CCGTAAATGCCTGAATCCTGGGGCTGCATGGCAGCCC‑CCCCGGTTTTCCCTTTACCCAATCCAGCCACt                                                                                                            <  4:6208/149‑1 (MQ=255)
                                      cccGGCGTCCGCGAGCCGCATTGCTTCGCTGCAGGCGGCGATTCCGAAGGTGATGCCGCTGTATCAGGCTGCCGCCAAG‑CCGTAAATGCCTGAATCCTGGGGCTGCATGGCAGCCC‑CCCCGGTTTTCCCTTTAc                                                                                                                          >  5:150240/1‑134 (MQ=255)
                                      cccGGCGTCCGCGAGCCGCATTGCTTCGCTGCAGGCGGCGATTCCGAAGGTGATGCCGCTGTATCAGGCTGCCGCCAAG‑CCGTAAATGCCTGAATCCTGGGGCTGCATGGCAGCCC‑CCCCGGTTTTCCCTTTAc                                                                                                                          <  6:150240/134‑1 (MQ=255)
                                      cccGGCGTCCGCGAGCCGCATTGCTTCGCTGCAGGCGGCGATTCCGAAGGTGATGCCGCTGTATCAGGCTGCCGCCAAG‑CCGTAAATGCCTGAATCCTGGGGCTGCATGGCAGCCC‑CCCCGGTTTTCCCTTTACCCAATCCAGCCACTg                                                                                                           <  2:210005/149‑1 (MQ=255)
                                                        cATTGCTTCGCTGCAGGCGGCGATTCCGAAGGTGATGCCGCTGTATCAGGCTGCCGCCAAG‑CCGTAAATGCCTGAATCCTGGGGCTGCATGGCAGCCC‑CCCCGGTTTTCCCTTTACCCAATCCAGCCACTGGCCTTCATTGCCGaa                                                                                            >  5:142152/1‑146 (MQ=255)
                                                        cATTGCTTCGCTGCAGGCGGCGATTCCGAAGGTGATGCCGCTGTATCAGGCTGCCGCCAAG‑CCGTAAATGCCTGAATCCTGGGGCTGCATGGCAGCCC‑CCCCGGGTTTCCCTTTACCCAATCCAGCCACTGGCCTTCATTGCCGAATAg                                                                                         >  4:54719/1‑149 (MQ=255)
                                                         aTTGCTTCGCTGCAGGCGGCGATTCCGAAGGTGATGCCGCTGTATCAGGCTGCCGCCAAG‑CCGTAAATGCCTGAATCCTGGGGCTGCATGGCAGCCC‑CCCCGGTTTTCCCTTTACCCAATCCAGCCAATGGCCTTCATTGCCGAATAGa                                                                                        >  2:84497/1‑149 (MQ=255)
                                                              ttCGCTGCAGGCGGCGATTCCGAAGGTGATGCCGCTGTATCAGGCTGCCGCCAAG‑CCGTAAATGCCTGAATCCTGGGGCTGCATGGCAGCCC‑CCCCGGTTTTCCCTTTACCCAATCCAGCCACTGGCCTTCATTGCCGAATAGACGGc                                                                                    >  5:339034/1‑148 (MQ=255)
                                                              ttCGCTGCAGGCGGCGATTCCGAAGGTGATGCCGCTGTATCAGGCTGCCGCCAAG‑CCGTAAATGCCTGAATCCTGGGGCTGCATGGCAGCCC‑CCCCGGTTTTCCCTTTACCCAATCCAGCCACTGGCCTTCATTGCCGAATAGACGGc                                                                                    >  6:42635/1‑148 (MQ=255)
                                                                  cTGCAGGCGGCGATTCCGAAGGTGATGCCGCTGTATCAGGCTGCCGCCAAG‑CCGTAAATGCCTGAATCCTGGGGCTGCATGGCAGCCC‑CCCCGGTTTTCCCTTTACCCAATCCAGCCACTGGCCTTCATTGCCg                                                                                              >  6:274633/1‑134 (MQ=255)
                                                                  cTGCAGGCGGCGATTCCGAAGGTGATGCCGCTGTATCAGGCTGCCGCCAAG‑CCGTAAATGCCTGAATCCTGGGGCTGCATGGCAGCCC‑CCCCGGTTTTCCCTTTACCCAATCCAGCCACTGGCCTTCATTGCCg                                                                                              <  5:274633/134‑1 (MQ=255)
                                                                     cAGGCGGCGATTCCGAAGGTGATGCCGCTGTATCAGGCTGCCGCCAAG‑CCGTAAATGCCTGAATCCTGGGGCTGCATGGCAGCCC‑CCCCGGTTTTCCCTTTACCCAATCCAGCCACTGGCCTTCATTGCCGAATAGACGGCCACGAtt                                                                             <  3:54719/148‑1 (MQ=255)
                                                                     cAGGCGGCGATTCCGAAGGTGATGCCGCTGTATCAGGCTGCCGCCAAG‑CCGTAAATGCCTGAATCCTGGGGCTGCATGGCAGCCC‑CCCCGGTTTTCCCTTTACCCAATCCAGCCAATGGCCTTCATTGCCGAATAGACGGCCACGAtt                                                                             <  1:84497/148‑1 (MQ=255)
                                                                      aGGCGGCGATTCCGAAGGTGATGCCGCTGTATCAGGCTGCCGCCAAG‑CCGTAAATGCCTGAATCCTGGGGCTGCATGGCAGCCC‑CCCCGGTTTTCCCTTTACCCAATCCAGCCACTGGCCTTCATTGCCGAATAGACGGCCACGATTGc                                                                           <  6:142152/149‑1 (MQ=255)
                                                                        gcggcgATTCCGAAGGTGATGCCGCTGTATCAGGCTGCCGCCAAG‑CCGTAAATGCCTGAATCCTGGGGCTGCATGGCAGCCC‑CCCCGGTTTTCCCTTTACCCAATCCAGCCACTGGCCTTCATTGCCGAATAGACGGCCACGATTGCCa                                                                         <  8:31719/149‑1 (MQ=255)
                                                                                 ccGAAGGTGATGCCGCTGTATCAGGCTGCCGCCAAG‑CCGTAAATGCCTGAATCCTGGGGCTGCATGGCAGCCC‑CCCCGGTTTTCCCTTTACCCAATCCAGCCACTGGCCTTCATTGCCGAATAGACGGCCACGATTGCCAGTACgaag                                                                 >  5:314674/1‑148 (MQ=255)
                                                                                   gAAGGTGATGCCGCTGTATCAGGCTGCCGCCAAG‑CCGTAAATGCCTGAATCCTGGGGCTGCATGGCAGCCC‑CCCCGGTTTTCCCTTTACCCAATCCAGCCACTGGCCTTCATTGCCGAATAGACGGCCACGATTGCCAGTACGAAGAAc                                                              <  6:314674/149‑1 (MQ=255)
                                                                                     aGGTGATGCCGCTGTATCAGGCTGCCGCCAAG‑CCGTAAATGCCTGAATCCTGGGGCTGCATGGCAGCCC‑CCCCGGTTTTCCCTTTACCCAATCCAGCCACTGGCCTTCATTGCCGAATAGACGGCCACGATTGCCAGTACGAAGAACGc                                                            <  1:79321/149‑1 (MQ=255)
                                                                                         gATGCCGCTGTATCAGGCTGCCGCCAAG‑CCGTAAATGCCTGAATCCTGGGGCTGCATGGCAGCCC‑CCCCGGTTTTCCCTTTACCCAATCCAGCCACTGGCCTTCATTGCCGAATAGACGGCCACGATTGCCAGTACGAAGAACGCCGtt                                                        >  3:50418/1‑149 (MQ=255)
                                                                                         gATGCCGCTGTATCAGGCTGCCGCCAAG‑CCGTAAATGCCTGAATCCTGGGGCTGCATGGCAGCCC‑CCCCGGTTTTCCCTTTACCCAATCCAGCCACTGGCCTTCATTGCCGAATAGACGGCCACGATTGCCAGTACGAAGAACGCCGtt                                                        >  8:263447/1‑149 (MQ=255)
                                                                                          aTGCCGCTGTATCAGGCTGCCGCCAAG‑CCGTAAATGCCTGAATCCTGGGGCTGCATGGCAGCCC‑CCCCGGTTTTCCCTTTACCCAATCCAGCCACTGGCCTTCATTGCCGAATAGACGGCCACGATTGCCAGTACGAAGAACGCCGTTg                                                       <  8:228890/149‑1 (MQ=255)
                                                                                                    aTCAGGCTGCCGCCAAG‑CCGTAAATGCCTGAATCCTGGGGCTGCATGGCAGCCC‑CCCCGGTTTTCCCTTTACCCAATCCAGCCACTGGCCTTCATTGCCGAATAGACGGCCACGATTGCCAGTACGAAGAACGCCGTTGCCGCCAggcg                                             >  5:182050/1‑149 (MQ=255)
                                                                                                     tCAGGCTGCCGCCAAG‑CCGTAAATGCCTGAATCCTGGGGCTGCATGGCAGCCCGCCCCGGTTTTCCCTTTACCCAATCCAGCCACTGGCCTTCATTGCCGAATAGACGGCCACGATTGCCAGTACGAAGAACGCCGTTGCCGCCAggcg                                             >  1:92377/1‑149 (MQ=255)
                                                                                                     tCAGGCTGCCGCCAAG‑CCGTAAATGCCTGAATCCTGGGGCTGCATGGCAGCCC‑CCCCGGTTTTCCCTTTACCCAATCCAGCCACTGGCCTTCATTGCCGAATAGACGGCCACGATTGCCAGTACGAAGAACGCCGTTGCCGCCAggcgg                                            >  1:14393/1‑149 (MQ=255)
                                                                                                     tCAGGCTGCCGCCAAG‑CCGTAAATGCCTGAATCCTGGGGCTGCATGGCAGCCC‑CCCCGGTTTTCCCTTTACCCAATCCAGCCACTGGCCTTCATTGCCGAATAGACGGCCACGATTACCAGTACGAAGAACGCCGTTGCCGCCAggcgg                                            >  5:273939/1‑149 (MQ=255)
                                                                                                       aGGCTGCCGCCAAG‑CCGTAAATGCCTGAATCCTGGGGCTGCATGGCAGCCC‑CCCCGGTTTTCCCTTTACCCAATCCAGCCACTGGCCTTCATTGCCGAATAGACGGCCACGATTGCCAGTACGAAGAACGCCGTTGCCGCCAggcggc                                           <  4:50418/148‑1 (MQ=255)
                                                                                                             ccgccAAG‑CCGTAAATGCCTGAATCCTGGGGCTGCATGGCAGCCC‑CCCCGGTTTTCCCTTTACCCAATCCAGCCACTGGCCTTCATTGCCGAATAGACGGCCACGATTGCCAGTACGAAGAACGCCGTTGCCGCCAGGCGGCGAATCAg                                    >  8:59006/1‑149 (MQ=255)
                                                                                                             ccgccAAG‑CCGTAAATGCCTGAATCCTGGGGCTGCATGGCAGCCC‑CCCCGGTTTTCCCTTTACCCAATCCAGCCACTGGCCTTCATTGCCGAATAGACGGCCACGATTGCCAGTACGAAGAACGCCGTTGCCGCCAGGCGGCGAATCAg                                    >  6:308892/1‑149 (MQ=255)
                                                                                                                 cAAG‑CCTTAAATGCCTGAATCCTGGGGCTGCATGGCAGCCC‑CCCCGGTTTTCCCTTTACCCAATCCAGCCACTGGCCTTCATTGCCGAATAGACGGCCACGATTGCCAGTACGAAGAACGCCg                                                          <  2:315298/123‑1 (MQ=255)
                                                                                                                       cGTAAATGCCTGAATCCTGGGGCTGCATGGCAGCCC‑CCCCGGTTTTCCCTTTACCCAATCCAGCCACTGGCCTTCATTGCCGAATAGACGGCCACGATTGCCAGTACGAAGAACGCCGTTGCCGCCAGGCGGCGAATCAGCGTCAACgg                           <  2:92377/149‑1 (MQ=255)
                                                                                                                         tAAATGCCTGAATCCTGGGGCTGCATGGCAGCCC‑CCCCGGTTTTCCCTTTACCCAATCCAGCCACTGGCCTTCATTGCCGAATAGACGGCCACGATTGCCAGTACGAAGAACGCCGTTGCCGCCAGGCGGCGAATCAGCGTCAACgg                           >  8:282525/1‑147 (MQ=255)
                                                                                                                              gCCTGAATCCTGGGGCTGCATGGCAGCCC‑CCCCGGTTTTCCCTTTACCCAATCCAGCCACTGGCCTTCATTGCCGAATAGACGGCCACGATTGCCAGTACGAAGAACGCCGTTGCCGCCAGGCGGCGAATCAGCGTCAACGGCAGTTTg                    >  4:333996/1‑149 (MQ=255)
                                                                                                                                cTGAATCCTGGGGCTGCATTGCACCCC‑CCCCCGTTTTACCGTTAACCAATACACCCACAGGCCATAATTTCCCAAAATACGGCCCCCATTGGCAGTAAGAAGAACCCCCTTTACCCCAGGCGGCGAAAAAACGTCAAACGCAGttttta                  >  2:341989/1‑146 (MQ=255)
                                                                                                                                cTGAATCCTGGGGCTGCATGGCAGCCC‑CCCCGGTTTTCCCTTTACCCAATCCAGCCACTGGCCTTCATTGCCGAATAGACGGCCACGATTGCCAGTACGAAGAACGCCGTTGCCGCCAGGCGGCGAATCAGCGTCCAc                             >  1:121770/1‑138 (MQ=255)
                                                                                                                                cTGAATCCTGGGGCTGCATGGCAGCCC‑CCCCGGTTTTCCCTTTACCCAATCCAGCCACTGGCCTTCATTGCCGAATAGACGGCCACGATTGCCAGTACGAAGAACGCCGTTGCCGCCAGGCGGCGAATCAGCGTCAAc                             <  2:121770/138‑1 (MQ=255)
                                                                                                                                cTGAATCCTGGGGCTGCATGGCAGCCC‑CCCCGGTTTTCCCTTTACCCAATCCAGCCACTGGCCTTCATTGCCGAATAGACGGCCACGATTGCCAGTACGAAGAACGCCGTTGCCGCCAGGCGGCGAATCAGCGTCAACGGCAGTTTGTc                  >  7:297641/1‑149 (MQ=255)
                                                                                                                                   aaTCCTGGGGCTGCATGGCAGCCC‑CCCCGGTTTTCCCTTTACCCAATCCAGCCACTGGCCTTCATTGCCGAATAGACGGCCACGATTACCAGTACGAAGAACGCCGTTGCCGCCAGGCGGCGAATCAGCGTCAACGGCAGTTTGTCTGc               <  6:273939/149‑1 (MQ=255)
                                                                                                                                     tCCTGGGGCTGCATGGCAGCCC‑CCCCGGTTTTCCCTTTACCCAATCCAGCCACTGGCCTTCATTGCCGAATAGACGGCCACGATTGCCAGTACGAAGAACGCCGTTGCCGCCAGGCGGCGAATCAGCGTCAACGGCAGTTTGTCTGc               >  4:345143/1‑147 (MQ=255)
                                                                                                                                     tCCTGGGGCTGCATGGCAGCCC‑CCCCGGTTTTCCCTTTACCCAATCCAGCCACTGGCCTTCATTGCCGAATAGACGGCCACGATTGCCAGTACGAAGAACGCCGTTGCCGCCAGGCGGCGAATCAGCGTCAACGGCAGTTTGTCTGc               <  3:345143/147‑1 (MQ=255)
                                                                                                                                     cgggggggCTGCCATGCAGCCC‑CCCCGGTTTTCCCTTTACCCAATCCAGCCACTGGCCTTCATTGCCGAATAGACGGCCACGATTGCCAGTACGAAGAACGCCGTTGCCGCCAGGCGGCGAATCAGCGTCAACGGCAGTTTGTCTGCcg             >  5:188754/5‑149 (MQ=255)
                                                                                                                                      gggggggCTGCCATGCAGCCC‑CCCCGGTTTTCCCGTTACCCAATCGAGCCACTGGCCTTCATTGCCGAATAGACGGCCACGATTGCCAGTACGAAGAACGCCGTTGCCGCCAGGCGGCGAATCAGCGTAAACGGCAGTATGACTGcc              >  3:28856/4‑147 (MQ=255)
                                                                                                                                        tGGGGCTGCATGGCAGCCC‑CCCCGGTTTTCCCTTTACCCAATCCAGCCACTGGCCTTCATTGCCGAATAGACGGCCACGATTGCCAGTACGAAGAACGCCGTTGCCGCCAGGCGGCGAATCAGCGTCAACGGCAGTTTGTCt                 >  3:94710/1‑142 (MQ=255)
                                                                                                                                        tGGGGCTGCATGGCAGCCC‑CCCCGGTTTTCCCTTTACCCAATCCAGCCACTGGCCTTCATTGCCGAATAGACGGCCACGATTGCCAGTACGAAGAACGCCGTTGCCGCCAGGCGGCGAATCAGCGTCAACGGCAGTTTGTCt                 <  4:94710/142‑1 (MQ=255)
                                                                                                                                          gggCTGCATGGCAGCCC‑CCCCGGTTTTCCCTTTACCCAATCCAGCCACTGGCCTTCATTGCCGAATAGACGGCCACGATTGCCAGTACGAAGAACGCCGTTGCCGCCAGGCGGCGAATCAGCGTCAACGGCAGTTTGTCTGCCGCGaa         <  3:333996/148‑1 (MQ=255)
                                                                                                                                          gggCTGCATGGCAGCCC‑CCCCGGTTTTCCCTTTACCCAATCCAGCCACTGGCCTTCATTGCCGAATAGACGGCCACGATTGCCAGTACGAAGAACGCCGTTGCCGCCAGGCGGCGAATCAGCGTCAACGGCAGTTTGTCTGCCGCGAAg        <  7:282525/149‑1 (MQ=255)
                                                                                                                                          gggCTGCATGGCAGCCC‑CCCCGGTTTTCCCTTTACCCAATCCAGCCACTGGCCTTCATTGCCGAATAGACGGCCACGATTGCCAGTACGAAGAACGCCGTTGCCGCCAGGCGGCGAATCAGCGTCAACGGCAGTTTGTCTGCCGCGAAg        <  5:42635/149‑1 (MQ=255)
                                                                                                                                                cATGGCAGCCC‑CCCCGGTTTTCCCTTTACCCAATCCAGCCACTGGCCTTCATTGCCGAATAGACGGCCACGATTGCCAGTACGAAGAACGCCGTTGCCGCCAGGCGGCGAATCAGCGTCAACGGCAGTTTGTCTGCCGCGAAGTTAccc  >  7:171112/1‑149 (MQ=255)
                                                                                                                                                cATGGCAGCCC‑CCCCGGTTTTCCCTTTACCCAATCCAGCCACTGGCCTTCATTGCCGAATAGACGGCCACGATTGCCAGTACGAAGAACGCCGTTGCCGCCAGGCGGCGAATCAGCGTCAACGGCAGTTTGTCTGCCGCGAAGTTAccc  <  2:14393/149‑1 (MQ=255)
                                                                                                                                                cATGGCAGCCC‑CCCCGGTTTTCCCTTTACCCAATCCAGCCACTGGCCTTCATTGCCGAATAGACGGCCACGATTGCCAGTACGAAGAACGCCGTTGCCGCCAGGCGGCGAATCAGCGTCAACGGCAGTTTGTCTGCCGCGAAGTTAcc   <  8:297641/148‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                  
GCTTCCGAAGGTGCACCGCCTGAGTTCATGAGTACTCACCCGGCGTCCGCGAGCCGCATTGCTTCGCTGCAGGCGGCGATTCCGAAGGTGATGCCGCTGTATCAGGCTGCCGCCAAG‑CCGTAAATGCCTGAATCCTGGGGCTGCATGGCAGCCC‑CCCCGGTTTTCCCTTTACCCAATCCAGCCACTGGCCTTCATTGCCGAATAGACGGCCACGATTGCCAGTACGAAGAACGCCGTTGCCGCCAGGCGGCGAATCAGCGTCAACGGCAGTTTGTCTGCCGCGAAGTTACCC  >  NC_002947/875139‑875430

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑