Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 916,642 T→A 9.7% I309F (ATT→TTT)  PP_0798 ← GGDEF domain‑containing protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_002947916,6420TA 9.7% 114.5 / 3.3 41I309F (ATT→TTT) PP_0798GGDEF domain‑containing protein
Reads supporting (aligned to +/- strand):  ref base T (15/22);  new base A (2/2);  total (17/24)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.95e-01

GCCCATTCCTGGCGCAGCACATGGTCCAGCCTGCGGCGGTTGGCCAGGCCAGTCAGGCTAT‑‑CGGTGGAAGCGAGCA‑T‑‑GGCCAGGTCTTCTTCGGCTTCGTGGCGGCGGCGCAGTTCGCGGCCCAGCAGCAATGTCAGCCAGAGAATGCCCACGCACAGACCGCCGGTGGCGACGCTGACCAGAATGGCGGTGCGGCGCCAGGACTCGAACACCTCGTCGGCCGAGTGCGCCACTAGCACGATCAGTGGCAACTGCGCGACCCGGGCGAAGGTGAACATGCG  >  NC_002947/916497‑916777
                                                                                                                                                      |                                                                                                                                       
gCCCATTCCTGGCGCAGCACATGGTCCAGCCTGCGGCGGTTGGCCAGGCCAGTCAGGCTAT‑‑CGGTGGAAGCGAGCA‑T‑‑GGCCAGGTCTTCTTCGGCTTCGTGGCGGCGGCGCAGTTCGCGGCCCAGCAGCAATGTCAGCCAGAGAATGcc                                                                                                                                      <  4:223016/149‑1 (MQ=255)
  ccATTCCTGGCGCAGCACATGGTCCAGCCTGCGGCGGTTGGCCAGGCCAGTCAGGCTAT‑‑CGGTGGAAGCGAGCA‑T‑‑GGCCAGGTCTTCTTCGGCTTCGTGGCGGCGGCGCAGTTCGCGGCCCAGCAGCAATGTCAGCCAGAGAATGCCCa                                                                                                                                    >  8:63769/1‑149 (MQ=255)
   cATTCCTGGCGCAGCACATGGTCCAGCCTGCGGCGGTTGGCCAGGCCAGTCAGGCTAT‑‑CGGTGGAAGCGAGCA‑T‑‑GGCCAGGTCTTCTTCGGCTTCGTGGCGGCGGCGCAGTTCGCGGCCCAGCAGCAATGTCAGCCAGAGAATGCCCAc                                                                                                                                   >  8:261778/1‑149 (MQ=255)
   cATTCCTGGCGCAGCACATGGTCCAGCCTGCGGCGGTTGGCCAGGCCAGTCAGGCTAT‑‑CGGTGGAAGCGAGCA‑T‑‑GGCCAGGTCTTCTTCGGCTTCGTGGCGGCGGCGCAGTTCGCGGCCCAGCAGCAATGTCAGCCAGAGAATGCCCAc                                                                                                                                   <  7:261778/149‑1 (MQ=255)
     ttCCTGGCGATGAACATGGTCCAGCCGGGGGGGGTTGGCCAGGCCTTTAAGGTTTTGGCAGTGGACGGGTGCACTCACGCCAGGTCTTCTTCGGCTTCGTGGCGGCGGCGCAGTTCGCGGCCCAGCAGCAATGTCAGCCAGAGAATGcc                                                                                                                                      <  1:282499/149‑1 (MQ=255)
                 cacaTGGTCCAGCCTGCGGCGGTTGGCCAGGCCAGTCAGGCTAT‑‑CGGTGGAAGCGAGCA‑T‑‑GGCCAGGTCTTCTTCGGCTTCGTGGCGGCGGCGCAGTTCGCGGCCCAGCAGCAATGTCAGCCAGAGAATGCCCACGCACAGACCGCCgg                                                                                                                     <  5:113196/149‑1 (MQ=255)
                   caTGGTCCAGCCTGCGGCGGTTGGCCAGGCCAGTCAGGCTAT‑‑CGGTGGAAGCGAGCA‑T‑‑GGCCAGGTCTTCTTCGGCTTCGTGGCGGCGGCGCAGTTCGCGGCCCAGCAGCAATGTCAGCCAGAGAATGCCCACGCACAGACCGCCGGTg                                                                                                                   <  2:71972/149‑1 (MQ=255)
                        tCCAGCCTGCGGCGGTTGGCCAGGCCAGTCAGGCTAT‑‑CGGTGGAAGCGAGCA‑T‑‑GGCCAGGTCTTCTTCGGCTTCGTGGCGGCGGCGCAGTTCGCGGCCCAGCAGCAATGTCAGCCAGAGAATGCCCACGCACAGACCGCCGGTGGCGAc                                                                                                              >  6:68594/1‑149 (MQ=255)
                                            caggccagTCAGGCTAT‑‑CGGTGGAAGCGAGCA‑T‑‑GGCCAGGTCTTCTTCGGCTTCGTGGCGGCGGCGCAGTTCGCGGCCCAGCAGCAATGTCAGCCAGAGAATGCCCACGCACAGACCGCCGGTGGCGACGCTGACCAGAATGGCGGt                                                                                            >  4:51308/1‑147 (MQ=255)
                                             aggccagTCAGGCTAT‑‑CGGTGGAAGCGAGCA‑T‑‑GGCCAGGTCTTCTTCGGCTTCGTGGCGGCGGCGCAGTTCGCGGCCCAGCAGCAATGTCAGCCAGAGAATGCCCa                                                                                                                                    <  5:221164/106‑1 (MQ=255)
                                             aggccagTCAGGCTAT‑‑CGGTGGAAGCGAGCA‑T‑‑GGCCAGGTCTTCTTCGGCTTCGTGGCGGCGGCGCAGTTCGCGGCCCAGCAGCAATGTCAGCCAGAGAATGCCCa                                                                                                                                    >  6:221164/1‑106 (MQ=255)
                                                         cTAT‑‑CGGTGGAAGCGAGCA‑T‑‑GGCCAGGTCTTCTTCGGCTTCGTGGCGGCGGCGCAGTTCGCGGCCCAGCAGCAATGTCAGCCAGAGAATGCCCACGCACAGACCGCCGGTGGCGACGCTGACCAGAATGGCgg                                                                                             >  6:317997/1‑133 (MQ=255)
                                                         cTAT‑‑CGGTGGAAGCGAGCA‑T‑‑GGCCAGGTCTTCTTCGGCTTCGTGGCGGCGGCGCAGTTCGCGGCCCAGCAGCAATGTCAGCCAGAGAATGCCCACGCACAGACCGCCGGTGGCGACGCTGACCAGAATGGCgg                                                                                             <  5:317997/133‑1 (MQ=255)
                                                            t‑‑CGGTGGAAGCGAGCA‑T‑‑GGCCAGGTCTTCTTCGGCTTCGTGGCGGCGGCGCAGTTCGCGGCCCAGCAGCAATGTCAGCCAGAGAATGCCCACGCACAGACCGCCGGTGGCGACGCTGACCAGAATGGCGGTGCGGCGCCAGGACTCGaa                                                                          <  7:63769/149‑1 (MQ=255)
                                                            t‑‑CGGTGGAAGCGAGCA‑T‑‑GGCCAGGTCTTCTTCGGCTTCGTGGCGGCGGCGCAGTTCGCGGCCCAGCAGCAATGTCAGCCAGAGAATGCCCACGCACAGACCGCCGGTGGCGACGCTGACCAGAATGGCGGTGCGGCGCCAGGACTCGaa                                                                          <  7:99784/149‑1 (MQ=255)
                                                            t‑‑CGGTGGAAGCGAGCA‑T‑‑GGCCAGGTCTTCTTCGGCTTCGTGGCGGCGGCGCAGTTCGCGGCCCAGCAGCAATGTCAGCCAGAGAATGCCCACGCACAGACCGCCGGTGGCGACGCTGACCAGAATGGCGGTGCGGCGCCAGGACTCGaa                                                                          <  5:68594/149‑1 (MQ=255)
                                                                         gctatct‑‑GGCCAGGGCTGCTTCGGCTTGGTGGCGGCGGCGCAGTTCGCGGCCCAGCAGCAATGTCAGACAGAGAATGCCCACGCACAGACCGCCGGTGGAGACGCTGACCAGAATGGCGGTGCGGCGCCAGGACTCGAACACCtcgtc                                                                 <  4:276986/142‑1 (MQ=255)
                                                                           gCA‑T‑‑GGCCAGGTCTTCTTCGGCTTCGTGGCGGCGGCGCAGTTCGCGGCCCAGCAGCAATGTCAGCCAGAGAATGCCCACGCACAGACCGCCGGTGGCGACGCTGACCAGAATGGCGGTGCGGCGCCAGGACTCGAACACCTCGTCGGcc                                                             <  3:51308/149‑1 (MQ=255)
                                                                                   gCCAGGTCTTCTTCGGCTTCGTGGCGGCGGCGCAGTTCGCGGCCCAGCAGCAATGTCAGCCAGAGAATGCCCACGCACAGACCGCCGGTGGCGACGCTGACCAGAATGGCGGTGCGGCGCCAGGACTCGAACACCTCGTCGGCCGAGTg                                                        >  7:9221/1‑149 (MQ=255)
                                                                                   gCCAGGTCTTCTTCGGCTTCGTGGCGGCGGCGCAGTTCGCGGCCCAGCAGCAATGTCAGCCAGAGAATGCCCACGCACAGACCGCCGGTGGCGACGCTGACCAGAATGGAGGTGCGGCGCCAGGACTCGAACAACtcgtc                                                                 >  3:276986/1‑140 (MQ=255)
                                                                                    ccAGGTCTTCTTCGGCTTCGTGGCGGCGGCGCAGTTCGCGGCCCAGCAGCAATGTCAGCCAGAGAATGCCCACGCACAGACCGCCGGTGGCGACGCTGACCAGAAt                                                                                                  <  2:20074/106‑1 (MQ=255)
                                                                                    ccAGGTCTTCTTCGGCTTCGTGGCGGCGGCGCAGTTCGCGGCCCAGCAGCAATGTCAGCCAGAGAATGCCCACGCACAGACCGCCGGTGGCGACGCTGACCAGAAt                                                                                                  >  1:20074/1‑106 (MQ=255)
                                                                                    ccAGGTCTTCTTCGGCTTCGTGGCGGCGGCGCAGTTCGCGGCCCAGCAGCAATGTCAGCCAGAGAATGCCCACGCACAGACCGCCGGTGGCGACGCTGACCAGAATGGCGGTgcggc                                                                                       <  4:44712/117‑1 (MQ=255)
                                                                                        gTCTTCTTCGGCTTCGTGGCGGCGGCGCAGTTCGCGGCCCAGCAGCAATGTCAGCCAGAGAATGCCCACGCACAGACCGCCGGTGGCGACGCTGACCAGAATGGCg                                                                                              <  1:206820/106‑1 (MQ=255)
                                                                                        gTCTTCTTCGGCTTCGTGGCGGCGGCGCAGTTCGCGGCCCAGCAGCAATGTCAGCCAGAGAATGCCCACGCACAGACCGCCGGTGGCGACGCTGACCAGAATGGCg                                                                                              >  2:206820/1‑106 (MQ=255)
                                                                                                  gCTTCGTGGCGGCGGCGCAGTTCGCGGCCCAGCAGCAATGTCAGCCAGAGAATGCCCACGCACAGACCGCCGGTGGCGACGCTGACCAGAATGGCGGTGCGGCGCCAGGACTCGAACACCtcg                                                                   >  4:64719/1‑123 (MQ=255)
                                                                                                  gCTTCGTGGCGGCGGCGCAGTTCGCGGCCCAGCAGCAATGTCAGCCAGAGAATGCCCACGCACAGACCGCCGGTGGCGACGCTGACCAGAATGGCGGTGCGGCGCCAGGACTCGAACACCtcg                                                                   <  3:64719/123‑1 (MQ=255)
                                                                                                        tGGCGGCGGCGCAGTTCGCGGCCCAGCAGCAATGTCAGCCAGAGAATGCCCACGCACAGACCGCCGGTGGCGACGCTGACCAGAATGGCGGTGCGGCGCCAg                                                                                  >  7:310736/1‑102 (MQ=255)
                                                                                                        tGGCGGCGGCGCAGTTCGCGGCCCAGCAGCAATGTCAGCCAGAGAATGCCCACGCACAGACCGCCGGTGGCGACGCTGACCAGAATGGCGGTGCGGCGCCAg                                                                                  <  8:310736/102‑1 (MQ=255)
                                                                                                          gcggcggcgCAGTTCGCGGCCCAGCAGCAATGTCAGCCAGAGAATGCCCACGCACAGACCGCCGGTGGCGACGCTGACCAGAATGGCGGTGCGGCGCCAGGACTCGAACACCtcgtcg                                                                <  4:317556/118‑1 (MQ=255)
                                                                                                          gcggcggcgCAGTTCGCGGCCCAGCAGCAATGTCAGCCAGAGAATGCCCACGCACAGACCGCCGGTGGCGACGCTGACCAGAATGGCGGTGCGGCGCCAGGACTCGAACACCtcgtcg                                                                >  3:317556/1‑118 (MQ=255)
                                                                                                                gcgcAGTTCGCGGCCCAGCAGCAATGTCAGCCAGAGAATGCCCACGCACAGACCGCCGGTGGCGACGCTGACCAGAATGGCGGTGCGGCGCCAGGACTCGAACACCTCGTCGGCCGAGTGCGCCACTAGCACGATCAGTGGCAACTgcg                           <  7:245530/149‑1 (MQ=255)
                                                                                                                       tCGCGGCCCAGCAGCAATGTCAGCCAGAGAAAGCCCACGCACAGACCGCCGGTGGCGACGCTGACCAGAATGGCGGTGCGGCGCCAGGACTCGAACACCTCGTCGGCCGAGTGCGCCACTAGCACGATCAGTGGCAACTGCGCGACCCg                    >  3:274250/1‑149 (MQ=255)
                                                                                                                       tCGCGGCCCAGCAGCAATGTCAGCCAGAGAAAGCCCACGCACAGACAGCCGGTGGCGACGCTGACCAGAATGGCGGTGCGGCGCCAGGACTCGAACACCTCGTCGGCCGAGTGCGCCACTAGCACGATCAGTGGCAACTGCGCGACCCg                    >  3:269589/1‑149 (MQ=255)
                                                                                                                              ccAGCAGCAATGTCAGCCAGAGAATGCCCACGCACAGACCGCCGGTGGCGACGCTGACCAGAATGGCGGTGCGGCGCCAGGACTCGAACACCTCGTCGGCCGAGTGCGCCACTAGCACGATCAGTGGCAACTGCGCGACCCggg                  <  6:29611/144‑1 (MQ=255)
                                                                                                                              ccAGCAGCAATGTCAGCCAGAGAATGCCCACGCACAGACCGCCGGTGGCGACGCTGACCAGAATGGCGGTGCGGCGCCAGGACTCGAACACCTCGTCGGCCGAGTGCGCCACTAGCACGATCAGTGGCAACTGCGCGACCCggg                  >  5:29611/1‑144 (MQ=255)
                                                                                                                                   agcaATGTCAGCCAGAGAAAGCCCACGCACAGACCGCCGGTGGCGACGCTGACCAGAATGGCGGTGCGGCGCCAGGACTCGAACACCTCGTCGGCCGAGTGCGCCACTAGCACGATCAGTGGCAACTGCGCGACCCGGGCGAAGGTGaa        <  4:274250/149‑1 (MQ=255)
                                                                                                                                   agcaATGTCAGCCAGAGAAAGCCCACGCACAGACCGCCGGTGGCGACGCTGACCAGAATGGCGGTGCGGCGCCAGGACTCGAACACCTCGTCGGCCGAGTGCGCCACTAGCACGATCAGTGGCAACTGCGCGACCCGGGCGAAGGTGaa        <  4:269589/149‑1 (MQ=255)
                                                                                                                                          tCAGCCAGAGAATGCCCACGCACAGACCGCCGGTGGCGACGCTGACCAGAATGGCTGTGCGGCGCCAGGACTCGAACACCTCGTCGGCCGAGTGCGCCACTAGGACGATCAGTGGCAACTGCGCGACCCGGGCGAAGGTGAACATgag  <  8:9221/148‑3 (MQ=255)
                                                                                                                                               cAGAGAATGCCCACGCACAGACCGCCGGTGGCGACGCTGACCAGAATGGCGGTGCGGCGCCAGGACTCGAACACCTCGTCGGCCGAGTGCGCCACTAGCAc                                            <  4:104978/101‑1 (MQ=255)
                                                                                                                                               cAGAGAATGCCCACGCACAGACCGCCGGTGGCGACGCTGACCAGAATGGCGGTGCGGCGCCAGGACTCGAACACCTCGTCGGCCGAGTGCGCCACTAGCAc                                            >  3:104978/1‑101 (MQ=255)
                                                                                                                                                      |                                                                                                                                       
GCCCATTCCTGGCGCAGCACATGGTCCAGCCTGCGGCGGTTGGCCAGGCCAGTCAGGCTAT‑‑CGGTGGAAGCGAGCA‑T‑‑GGCCAGGTCTTCTTCGGCTTCGTGGCGGCGGCGCAGTTCGCGGCCCAGCAGCAATGTCAGCCAGAGAATGCCCACGCACAGACCGCCGGTGGCGACGCTGACCAGAATGGCGGTGCGGCGCCAGGACTCGAACACCTCGTCGGCCGAGTGCGCCACTAGCACGATCAGTGGCAACTGCGCGACCCGGGCGAAGGTGAACATGCG  >  NC_002947/916497‑916777

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑