Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 922,774 C→A 6.2% R85L (CGC→CTC)  PP_0803 ← protein secretion ABC transporter permease

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_002947922,7740CA 6.2% 204.4 / 3.3 64R85L (CGC→CTC) PP_0803protein secretion ABC transporter permease
Reads supporting (aligned to +/- strand):  ref base C (28/32);  new base A (2/2);  total (30/34)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.41e-01

CGCAGGCTCTCCGGGAAGGTCAGTGGCTTGCCGGTCACCTCTGCCTGCAGGCGGGCCTGGCTGGCCCTGGCAGCGCGGTACTTGGCCTCGCTTTCACCCACGCTGGAAGCCGTCTTGGTCGGGTCGAGCTGGGCCAGCACCTGGCCGCGCTCGACCAGGTCACCTTCGGCCACGCTCATCTGCGCGACGATACCGCCCTCGAACGACTGAATGACCTGCTCTCGCGAACTTGGAATGACCTTGCCTGTGCCGGTGGATACCTCGGTCACCTCGAACCAGGCCGCCCAGGC  >  NC_002947/922627‑922916
                                                                                                                                                   |                                                                                                                                              
cgcAGGCTCTCCGGGAAGGTCAGTGGCTTGCCGGTCACCTCTGCCTGCAGGCGGGCCTGGCTGGCCCTGGCAGCGCGGTACTTGGCCTCGCTTTCACCCACGCTGGAAGCCGTCTTGGTCGGGTCGAGCTGGGCCAGCACCTGGCcgcg                                                                                                                                               >  5:321213/1‑149 (MQ=255)
      ctctCCGGGAAGGTCAGTGGCTTGCCGGTCACCTCTGCCTGCAGGCGGGCCTGGCTGGCCCTGGCAGCGCGGTACTTGGCCTCGCTTTCACCCACGCTGGAAGCCGTCTTGGTCGGGTCGAGCTGGGCCAGCACCTGGCCGCGCTCGAc                                                                                                                                         <  8:191380/149‑1 (MQ=255)
      ctcgCCGGGAAGGGCAGTGGCTTGCCGGTCACCTCTGCCTACAGGCGGGCCTGGCTGGCCCTGGCAGCGCGGTACGTGGCCTCGCTTTCACCCACGCTGGAAGCCGTCTTGGTCGGGTCGAGCGCGGCCAGGTGCTGGCCCAGCTCGAc                                                                                                                                         <  6:277488/149‑1 (MQ=255)
          ccGGGAAGGTCAGTGGCTTGCCGGTCACCTCTGCCTGCAGGCGGGCCTGGCTGGCCCTGGCAGCGCGGTACTTGGCCTCGCTTTCACCCACGCTGGAAGCCGTCTTGGTCGGGTCGCGCTGGGCCAGCACCTGGCCGCGCTCGACCAgg                                                                                                                                     >  4:132172/1‑149 (MQ=255)
           cGGGAAGGTCAGTGGCTTGCCGGTCACCTCTGCCTGCAGGCGGGCCTGGCTGGCCCTGGCAGCGCGGTACTTGGCCTCGCTTTCACCCACGCTGGAAGCCGTCTTGGTCGGGTCGAGCTGGGCCAGCACCTGGCCGCGCTCGACCAGGt                                                                                                                                    >  4:270087/1‑149 (MQ=255)
                 ggTCAGTGGCTTGCCGGTCACCTCTGCCTGCAGGCGGGCCTGGCTGGCCCTGGCAGCGCGGTACTTGGCCTCGCTTTCACCCACGCTGGAAGCCGTCTTGGTCGGGTCGAGCTGGGCCAGCACCTGGCCGCGCTCGACCAGGTCACCtt                                                                                                                              >  5:310270/1‑149 (MQ=255)
                  gTCAGTGGCTTGCCGGTCACCTCTGCCTGCAGGCGGGCCTGGCTGGCCCTGGCAGCGCGGTACTTGGCCTCGCTTTCACCCACGCTGGAAGCCGTCTTGGTCGGGTCGAGCTGGGCCAGCACCTGGCCGCGCTCGACCAGGTCACCTTc                                                                                                                             >  5:82621/1‑149 (MQ=255)
                   tCAGTGGCTTGCCGGTCACCTCTGCCTGCAGGCGGGCCTGGCTGGCCCTGGCAGCGCGGTACTTGGCCTCGCTTTCACCCACGCTGGAAGCCGTCTTGGTCGGGTCGAGCTGGGCCAGCACCTGGCCGCGCTCGACCAGGTCACCTTCg                                                                                                                            <  6:295053/149‑1 (MQ=255)
                    cAGTGGCTTGCCGGTCACCTCTGCCTGCAGGCGGGCCTGGCTGGCCCTGGCAGCGCGGTACTTGGCCTCGCTTTCACCCACGCTGGAAGCCGTCTTGGTCGGGTCGAGCTGGGCCAGCACCTGGCCGCGCTCGACCAGGTCACCTTCgg                                                                                                                           >  8:78772/1‑149 (MQ=255)
                               cGGTCACCTCTGCCTGCAGGCGGGCCTGGCTGGCCCTGGCAGCGCGGTACTTGGCCTCGCTTTCACCCACGCTGGAAGCCGTCTTGGTCGGGTCGAGCTGGGCCAGCACCTGGCCGCGCTCGACCAGGTCACCTTCGGCCACGCTCATc                                                                                                                >  2:71992/1‑149 (MQ=255)
                                      ctctGCCTGCAGGCGGGCCTGGCTGGCCCTGGCAGCGCGGTACTTGGCCTCGCTTTCACCCACGCTGGAAGCCGTCTTGGTCGGGTCGAGCTGGGCCAGCACCTGGCCGCGCTCGACCAGGTCACCTTCGGCCACGCTCATCTGCGcga                                                                                                         >  3:344170/1‑149 (MQ=255)
                                       tctGCCTGCAGGCGGGCCTGGCTGGCCCTGGCAGCGCGGTACTTGGCCTCGCTTTCACCCACGCTGGAAGCCGTCTTGGTCGGGTCGAGCTGGGCCAGCACCTGGCCGCGCTCGACCAGGTCACCTTCGGCCACGCTCATCTGCGcgac                                                                                                        >  2:233605/1‑149 (MQ=255)
                                           cctgcAGGCGGGCCTGGCTGGCCCTGGCAGCGCGGTACTTGGCCTCGCTTTCACCCACGCTGGAAGCCGTCTTGGTCGGGTCGAGCTGGGCCAGCACCTGGCCGCGCTCGACCAGGTCACCTTCGGCCACGCTCATCTGCGcgacga                                                                                                      <  4:344170/147‑1 (MQ=255)
                                                  gCGGGCCTGGCTGGCCCTGGCAGCGCGGTACTTGGCCTCGCTTTCACCCACGCTGGAAGCCGTCTTGGTCGGGTCGAGCTGGGCCAGCACCTGGCCGCGCTCGACCAGGTCACCTTCGGCCACGCTCATCTGCGCGACGATACCGCCCt                                                                                             >  4:173404/1‑149 (MQ=255)
                                                   cGGGCCTGGCTGGCCCTGGCAGCGCGGTACTTGGCCTCGCTTTCACCCACGCTGGAAGCCGTCTTGGTCGGGTCGAGCTGGGCCAGCACCTGGCCGCGCTCGACCAGGTCACTTTCGGCCACGCTCATCTGCGCGACGATACCGCCCTc                                                                                            <  1:233605/149‑1 (MQ=255)
                                                       ccTGGCTGGCCCTGGCAGCGCGGTACTTGGCCTCGCTTTCACCCACGCTGGAAGCCGTCTTGGTCGGGTCGAGCTGGGCCAGCACCTGGCCGCGCTCGACCAGGTCACCTTCGGCCACGCTCATCTGCGCGACGATACCGCCCTCGAAc                                                                                        <  7:78772/149‑1 (MQ=255)
                                                          ggctggcCCTGGCAGCGCGGTACTTGGCCTCGCTTTCACCCACGCTGGAAGCCGTCTTGGTCGGGTCGAGCTGGGCCAGCACCTGGCCGCGCTCGACCAGGTCACCTTCGGCCACGCTCATCTGCGCGACGATACCGCCCTCGAACGAc                                                                                     <  6:321213/149‑1 (MQ=255)
                                                             tggcCCTGGCAGCGCGGTACTTGGCCTCGCTTTCACCCACGCTGGAAGCCGTCTTGGTCGGGTCGAGCTGGGCCAGCACCTGGCCGCGCTCGACCAGGTCACCTTCGGCCACGCTCATCTGCGCGACGATACCGCCCTCGAACGACTg                                                                                   >  8:201615/1‑148 (MQ=255)
                                                                 ccTGGCAGCGCGGTACTTGGCCTCGCTTTCACCCACGCTGGAAGCCGTCTTGGTCGGGTCGAGCTGGGCCAGCACCTGGCCGCGCTCGACCAGGTCACCTTCGGc                                                                                                                          <  7:291990/105‑1 (MQ=255)
                                                                 ccTGGCAGCGCGGTACTTGGCCTCGCTTTCACCCACGCTGGAAGCCGTCTTGGTCGGGTCGAGCTGGGCCAGCACCTGGCCGCGCTCGACCAGGTCACCTTCGGc                                                                                                                          >  8:291990/1‑105 (MQ=255)
                                                                      cAGCGCGGTACTTGGCCTCGCTTTCACCCACGCTGGAAGCCGTCTTGGTCGGGTCGAGCTGGGCCAGCACCTGGCCGCGCTCGACCAGGTCACCTTCGGCCACGCTCATCTGCGCGACGATACCGCCCTCGAACGACTGAATGACCTGc                                                                         <  3:270087/149‑1 (MQ=255)
                                                                          gcgGTACTTGGCCTCGCTTTCACCCACGCTGGAAGCCGTCTTGGTCGGGTCGAGCTGGGCCAGCACCTGGCCGCGCTCGACCAGGTCACCTTCGGCCACGCTCATCTGCGCGACGATACCGCCCTCGAACGACTGAATGACCTGCtctc                                                                     >  5:311058/1‑149 (MQ=255)
                                                                            ggTACTTGGCCTCGCTTTCACCCACGCTGGAAGCCGTCTTGGTCGGGTCGAGCTGGGCCAGCACCTGGCCGCGCTCGACCAGGTCACCTTCGGCCACGCTCATCTGCGCGACGATACCGCCCTCGAACGACTGAATGACCTGCTCTcgc                                                                   <  7:201615/149‑1 (MQ=255)
                                                                                  tGGCCTCGCTTTCACCCACGCTGGAAGCCGTCTTGGTCGGGTCGAGCTGGGCCAGCACCTGGCCGCGCTCGACCAGGTCACCTTCGGCCACGCTCATCTGCGCGACGATACCGCCCTCGAACGACTGAATGACCTGCTCTCGCGAACtt                                                             >  2:89806/1‑149 (MQ=255)
                                                                                   ggCCTCGCTTTCACCCACGCTGGAAGCCGTCTTGGTCGGGTCGAGCTGGGCCAGCACCTGGCCGCGCTCGACCAGGTCACCTTCGGCCACGCTCATCTGCGCGACGATACCGCCCTCGAACGACTGAATGACCTGCTCTCGCGAACTTg                                                            >  7:34495/1‑149 (MQ=255)
                                                                                   ggCCTCGCTTTCACCCACGCTGGAAGCCGTCTTGGTCGGGTCGAGCTGGGCCAGCACCTGGCCGCGCTCGACCAGGTCACCTTAGGCCACGCTCATCTGCGCGACGATACCGCCCTCGAACGACTGAATGACCTGCTCTCGCGAACTTg                                                            >  1:141484/1‑149 (MQ=255)
                                                                                      cTCGCTTTCACCCACGCTGGAAGCCGTCTTGGTCGGGTCGAGCTGGGCCAGCACCTGGCCGCGCTCGACCAGGTCACCTTCGGCCACGCTCATCTGCGCGCCGATACCGCCCTCGAACGACTGAATGACCTGCTCTCGCGAACTTGGaa                                                         >  7:119607/1‑149 (MQ=255)
                                                                                      cTCGCTTTCACCCACGCTGGAAGCCGTCTTGGTCGGGTCGAGCTGGGCCAGCACCTGGCCGCGCTCGACCAGGTCACCTTCGGCCACGCTCATCTGCGCGACGATACCGCCCTCGAACGACTGAATGACCTGCTCTCGCGAACTTGGaa                                                         <  1:71992/149‑1 (MQ=255)
                                                                                       tCGCTTTCACCCACGCTGGAAGCCGTCTTGGTCGGGTCGAGCTGGGCCAGCACCTGGCCGCGCTCGACCAGGTCACCTTCGGCCACGCTCATCTGCGCGACGATACCGCCCTCGAACGACTGAATGACCTGCTCTcgc                                                                   <  4:304402/138‑1 (MQ=255)
                                                                                       tCGCTTTCACCCACGCTGGAAGCCGTCTTGGTCGGGTCGAGCTGGGCCAGCACCTGGCCGCGCTCGACCAGGTCACCTTCGGCCACGCTCATCTGCGCGACGATACCGCCCTCGAACGACTGAATGACCTGCTCTcgc                                                                   >  3:304402/1‑138 (MQ=255)
                                                                                       tCGCTTTCACCCACGCTGGAAGCCGTCTTGGTCGGGTCGAGCTGGGCCAGCACCTGGCCGCGCTCGACCAGGTCACCTTCGGCCACGCTCATCTGCGCGACGATACCGCCCTCGAACGACTGAATGACCTGCTCTCGCGAACTTGGAAt                                                        >  1:155606/1‑149 (MQ=255)
                                                                                         gCTTTCACCCACGCTGGAAGCCGTCTTGGTCGGGTCGAGCTGGGCCAGCACCTGGCCGCGCTCGACCAGGTCACCTTCGGCCACGCTCATCTGCGCGACGATACCGCCCTCGAACGACTGAATGACCTGCTCTCGCGAACTTGGAAt                                                        <  5:259304/147‑1 (MQ=255)
                                                                                              cACCCACGCTGGAAGCCGTCTTGGTCGGGTCGAGCTGGGCCAGCACCTGGCCGCGCTCGACCAGGTCACCTTCGGCCACGCTCATCTGCGCGACGATACCGCCCTCGAACGACTGAATGACCTGCTCTCGCGAACTTGGAATGACCtt                                                  <  6:82621/148‑1 (MQ=255)
                                                                                               aCCCACGCTGGAAGCCGTCTTGGTCGGGTCGAGCTGGGCCAGCACCTGGCCGCGCTCGACCAGGTCACCTTCGGCCACGCTCATCTGCGCGACGATACCGCCCTCGAAc                                                                                        <  2:291143/109‑1 (MQ=255)
                                                                                               aCCCACGCTGGAAGCCGTCTTGGTCGGGTCGAGCTGGGCCAGCACCTGGCCGCGCTCGACCAGGTCACCTTCGGCCACGCTCATCTGCGCGACGATACCGCCCTCGAAc                                                                                        >  1:291143/1‑109 (MQ=255)
                                                                                                cccACGCTGGAAGCCGTCTTGGTCGGGTCGAGCTGGGCCAGCACCTGGCCGCGCTCGACCAGGTCACCTTCGGCCACGCTCATCTGCGCGACGATACCGCCCTCGAACGACTGAATGACCTGCTCTCGCGAACTTGGAATGACCTTGcc                                               <  2:141484/149‑1 (MQ=255)
                                                                                                cccACGCTGGAAGCCGTCTTGGTCGGGTCGAGCTGGGCCAGCACCTGGCCGCGCTCGACCAGGTCACCTTCGGCCACGCTCATCTGCGCCACGATACCGCCCTCGAACGACTGAATGACCTGCTCTCGCGAACTTGGAATGACCTTGc                                                >  2:339057/1‑148 (MQ=255)
                                                                                                 ccACGCTGGAAGCCGTCTTGGTCGGGTCGAGCTGGGCCAGCACCTGGCCGCGCTCGACCAGGTCACCTTCGGCCACGCTCATCTGCGCGACGATACCGCCCTCGAACGACTGAATGACCTGCTCTCGCGAACTTGGAATGACCTTGCCt                                              >  7:247535/1‑149 (MQ=255)
                                                                                                     gCTGGAAGCCGTCTTGGTCGGGTCGAGCTGGGCCAGCACCTGGCCGCGCTCGACCAGGTCACCTTCGGCCACGCTCATCTGCGCGACGATACCGCCCTCGAACGACTGAATGACCTGCTCTCGCGAACTTGGAATGACCTTGCCTGTGc                                          <  1:339057/149‑1 (MQ=255)
                                                                                                           aGCCGTCTTGGTCGGGTCGAGCTGGGCCAGCACCTGGCCGCGCTCGACCAGGTCACCTTCGGCCACGCTCATCTGCGCGACGATACCGCCCTCGAACGACTGAATGACCTGCTCTCGCGAACTTGGAATGACCTTGCCTGTGCCGGTg                                     >  6:74956/1‑148 (MQ=255)
                                                                                                                  ttGGTCGGGTCGAGCTGGGCCAGCACCTGGCCGCGCTCGACCAGGTCACCTTCGGCCACGCTCATCTGCGCGACGATACCGCCCTCGAACGACTGAATGACCTGCTCTCGCGAACTTGGAATGACCTTGCCTGTGCCGGTGGATACCTc                             <  8:110236/149‑1 (MQ=255)
                                                                                                                    ggtcgggtcgAGCTGGGCCAGCACCTGGCCGCGCTCGACCAGGTCACCTTCGGCCACGCTCATCTGCGCGACGATACCGCCCTCGAACGACTGAATGACCTGCTCTCGCGAACTTGGAATGACCTTGCCTGTGCCGGTGGATACCTCg                            <  8:34495/148‑1 (MQ=255)
                                                                                                                    ggtcgggtcgAGCTGGGCCAGCACCTGGCCGCGCTCGACCAGGTCACCTTCGGCCACGCTCATCTGCGCGACGATACCGCCCTCGAACGACTGAATGACCTGCTCTCGCGAACTTGGAATGACCTTGCCTGTGCCGGTGGATACCTCg                            <  2:155606/148‑1 (MQ=255)
                                                                                                                     gtcgggtcgAGCTGGGCCAGCACCTGGCCGCGCTCGACCAGGTCACCTTCGGCCACGCTCATCTGCGCGACGATACCGCCCTCGAACGACTGAATGACCTGCTCTCGCGAACTTGGAATGACCTTGCCTGTGCCGGTGGAt                                  <  3:47023/141‑1 (MQ=255)
                                                                                                                     gtcgggtcgAGCTGGGCCAGCACCTGGCCGCGCTCGACCAGGTCACCTTAGGCCACGCGCATCTGCGCGACGATACCCCCCTCGAACGACTGAATGACCTGCTCAGGCGAACTTGGAATGAACTTGCCTGTGCCCGTGGATaatatag                           >  4:47023/1‑142 (MQ=255)
                                                                                                                      tcgggtcgAGCTGGGCCAGCACCTGGCCGCGCTCGACCAGGTCACCTTCGGCCACGCTCATCTGCGCGACGATACCGCCCTCGAACGACTGAATGACCTGCTCTCGCGAACTTGGAATGACCTTGCCTGTGCCGGTGGATACCTCGGTc                         <  6:311058/149‑1 (MQ=255)
                                                                                                                        tggtcgAGCGCGGCCAGGTGCTGGCCCAGCTCGACCAGGTCACCTTCGGCCACGCTCATCTGCGCGACGATACCGCCCTCGAACGACTGAATGACCTGCTCTCGCGAACtt                                                             <  6:297756/110‑1 (MQ=37)
                                                                                                                        tggtcgAGCGCGGCCAGGTGCTGGCCCAGCTCGACCAGGGCACCTTCGGCCACGCTCATCTGCGCGACGATACCGCCCTCGAACGACTGAATGACCTGCTCTCGCGAACtt                                                             >  5:297756/2‑111 (MQ=37)
                                                                                                                        gggtcgAGCTGGGCCAGCACCTGGCCGCGCTCGACCAGGTCACCTTCGGCCACGCTCATCTGCGCGACGATACCGCCCTCGAACGACTGAATGACCTGCTCTCGCGAACTTGGAATGACCTTGCCTGTGCCGGTGGATACCTCGGTCAc                       <  8:247535/149‑1 (MQ=255)
                                                                                                                           tcgAGCTGGGCCAGCACCTGGCCGCGCTCGACCAGGTCACCTTCGGCCACGCTCATCTGCGCGACGATACCGCCCTCGAACGACTGAATGACCTGCTCTCGCGAACTTGGAATGACCTTGCCTGTGCCGGTGGATACCTCGGTCACCTc                    >  4:241740/1‑149 (MQ=255)
                                                                                                                               gCTGGGCCAGCACCTGGCCGCGCTCGACCAGGTCACCTTCGGCCACGCTCATCTGCGCGACGATACCGCCCTCGAACGACTGAATGACCTGCTCTCGCGAACTTGGAATGACCTTGCCTGTGCCGGTGGATACCTCGGTCACCTCGAAc                <  6:310270/149‑1 (MQ=255)
                                                                                                                               gCTGGGCCAGCACCTGGCCGCGCTCGACCAGGTCACCTTCGGCCACGCTCATCTGCGCGACGATACCGCCCTCGAACGACTGAATGACCTGCTCTCGCGAACTTGGAATGACCTTGCCTGTGCCGGTGGATACCTCGGTCACCTCGAAc                >  7:212224/1‑149 (MQ=255)
                                                                                                                                 tGGGCCAGCACCTGGCCGCGCTCGACCAGGTCACCTTCGGCCACGCTCATCTGCGCGACGATACCGCCCTCGAACGACTGAATGACCTGCTCTCGCGAACTTGGAATGACCTTGCCTGTGCCGGTGGATACCTCGGTCACCTCGAACCa              <  8:212224/149‑1 (MQ=255)
                                                                                                                                   ggCCAGCACCTGGCCGCGCTCGACCAGGTCACCTTCGGCCACGCTCATCTGCGCGACGATACCGCCCTCGAACGACTGAATGACCTGCTCTCGCGAACTTGGAATGACCTTGCCTGTGCCGGTGGATACCTCGGTCACCt                     <  8:245843/140‑1 (MQ=255)
                                                                                                                                   ggCCAGCACCTGGCCGCGCTCGACCAGGTCACCTTCGGCCACGCTCATCTGCGCGACGATACCGCCCTCGAACGACTGAATGACCTGCTCTCGCGAACTTGGAATGACCTTGCCTGTGCCGGTGGATACCTCGGTCACCt                     >  7:245843/1‑140 (MQ=255)
                                                                                                                                   ggCCAGCACCTGGCCGCGCTCGACCAGGTCACCTTCGGCCACGCTCATCTGCGCGACGATACCGCCCTCGAACGACTGAATGACCTGCTCTCGCGAACTTGGAATGACCTTGCCTGTGCCGGTGGATACCTCGGTCACCTCGAACCAgg            <  5:74956/149‑1 (MQ=255)
                                                                                                                                   ggCCAGCACCTGGCCGCGCTCGACCAGGTCACCTTCGGCCACGCTCATCTGCGCGACGATACCGCCCTCGAACGACTGAATGACCTGCTCTCGCGAACTTGGAATGACCTTGCCTGTGCCGGTGGATACCTCGGTCACCTCGAACCAgg            <  3:173404/149‑1 (MQ=255)
                                                                                                                                     ccAGCACCTGGCCGCGCTCGACCAGGTCACCTTCGGCCACGCTCATCTGCGCGACGATACCGCCCTCGAACGACTGAATGACCTGCTCTCGCGAACTTGGAATGACCTTGCCTGTGCCGGt                                      <  2:256407/121‑1 (MQ=255)
                                                                                                                                     ccAGCACCTGGCCGCGCTCGACCAGGTCACCTTCGGCCACGCTCATCTGCGCGACGATACCGCCCTCGAACGACTGAATGACCTGCTCTCGCGAACTTGGAATGACCTTGCCTGTGCCGGTGGATACCTCGGTCACCTCGAACCAGgcc          >  5:47286/1‑149 (MQ=255)
                                                                                                                                        tgaCCTGGTCGAGCGCGGCCAGGTCACCTTCGGCCACGCTCATCTGCGCGACGATACCGCCCTCGAACGACTGAATGACCTGCTCTCGCGAACTTGGAATGACCTTGCCTGTGCCGGTGGATACCTCGGTCACCTCGAACCAGgccgcc       >  6:301634/3‑149 (MQ=255)
                                                                                                                                         cACCTGGCCGCGCTCGACCAGGTCACCTTCGGCCACGCTCATCTGCGCGACGATACCGCCCTCGAACGACTGAATGACCTGCTCTCGCGAACTTGGAATGACCTTGCCTGTGCCGGTGGATACCTCGGTCACCTCGAACCa              <  2:260260/141‑1 (MQ=255)
                                                                                                                                         cACCTGGCCGCGCTCGACCAGGTCACCTTCGGCCACGCTCATCTGCGCGACGATACCGCCCTCGAACGACTGAATGACCTGCTCTCGCGAACTTGGAATGACCTTGCCTGTGCCGGTGGATACCTCGGTCACCTCGAACCa              >  1:260260/1‑141 (MQ=255)
                                                                                                                                          aCCTGGCCGCGCTCGACCAGGTCACCTTCGGCCACGCTCATCTGCGCGACGATACCGCCCTCGAACGACTGAATGACCTGCTCTCGCGAACTTGGAATGACCTTGCCTGTGCCGGTGGATACCTCGGTCACCTCGAACCAGGCCGCcca     <  6:47286/149‑1 (MQ=255)
                                                                                                                                           ccTGGCCGCGCTCGACCAGGTCACCTTCGGCCACGCTCATCTGCGCGACGATACCGCCCTCGAACGACTGAATGACCTGCTCTCGCGAACTTGGAATGACCTTGCCTGTGCCGGTGGATACCTCGGTCACCTCGAACCAGGCCGCccag    <  1:89806/149‑1 (MQ=255)
                                                                                                                                               gCCGCGCTCGACCAGGTCACCTTCGGCCACGCTCATCTGCGCGACGATACCGCCCTCGAACGACTGAATGACCTGCTCTCGCGAACTTGGAATGACCTTGCCTGTGCCGGTGGATACCTCGGTCACCTCGAACCAGGCCGCccaggc  <  3:241740/147‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                              
CGCAGGCTCTCCGGGAAGGTCAGTGGCTTGCCGGTCACCTCTGCCTGCAGGCGGGCCTGGCTGGCCCTGGCAGCGCGGTACTTGGCCTCGCTTTCACCCACGCTGGAAGCCGTCTTGGTCGGGTCGAGCTGGGCCAGCACCTGGCCGCGCTCGACCAGGTCACCTTCGGCCACGCTCATCTGCGCGACGATACCGCCCTCGAACGACTGAATGACCTGCTCTCGCGAACTTGGAATGACCTTGCCTGTGCCGGTGGATACCTCGGTCACCTCGAACCAGGCCGCCCAGGC  >  NC_002947/922627‑922916

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑