Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 994,113 C→G 7.0% Q374E (CAG→GAG) ‡ PP_0858 → methionine/glutamine aminotransferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_002947994,1130CG 7.0% 184.6 / 4.2 57Q374E (CAG→GAG) ‡PP_0858methionine/glutamine aminotransferase
Reads supporting (aligned to +/- strand):  ref base C (27/26);  new base G (2/2);  total (29/28)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.59e-01

CCCGGACCTGAACGATGTCGACATGTCGCTGTGGCTGACGCGTGAGCACGGCGTTGCGACCATCCCGGTATCGGTGTTCTACCAGCAACCCATCCCCGAGCAACGCCTGGTACGCCTGTGCTTTGCCAAACGAGAGGAGACGCTGCGTCAGGCAGCGGAACGACTATGCGCGATCTGAGTACACTGCCGAACCTGAAAGTCGCCCTGGTGCAAACCACCTTGGCCTGGCACGACCGCGAGGCCAACTACGCGCACTTCGAGGTGCTGCTGGAGCAGGTGGGCGAGGTCGACCTGGT  >  NC_002947/993965‑994260
                                                                                                                                                    |                                                                                                                                                   
cccGGACCTGAACGATGTCGACATGTCGCTGTGGCTGACGCGTGAGCACGGCGTTGCGACCATCCCGGTATCGGTGTTCTACCAGCAACCCATCCCCGAGCAACTCCTGGTACGCCTGTGCTTTGCCAAACCAGAGGAGACGCTGCGTc                                                                                                                                                     >  7:146366/1‑149 (MQ=255)
        tGAACGATGTCGACATGTCGCTGTGGCTGACGCGTGAGCACGGCGTTGCGACCATCCCGGTATCGGTGTTCTACCAGCAACCCATCCCCGAGCAACGCCTGGTACGCCTGTGCTTTGCCAAACGAGAGGAGACGCTGCGTCAGGCAGCg                                                                                                                                             >  3:174145/1‑149 (MQ=255)
            cGATGTCGACATGTCGCTGTGGCTGACGCGTGAGCACGGCGTTGCGACCATCCCGGTATCGGTGTTCTACCAGCAACCCATCCCCGAGCAACGCCTGGTACGCCTGTGCTTTGCCAAACGAGAGGAGACGCTGCCTGACGCAGCgtctc                                                                                                                                         <  4:78297/149‑5 (MQ=255)
                 tCGACATGTCGCTGTGGCTGACGCGTGAGCACGGCGTTGCGACCATCCCGGTATCGGTGTTCTACCAGCAACCCATCCCCGAGCAACGCCTGGTACGCCTGTGCTTTGCCAAACGAGAGGAGACGCTGCGTCAGGCAGCGGAACGACt                                                                                                                                     <  8:316659/148‑1 (MQ=255)
                    aCATGTCGCTGTGGCTGACGCGTGAGCACGGCGTTGCGACCATCCCGGTATCGGTGTTCTACCAGCAACCCATCCCCGAGCAACGCCTGGTACGCCTGTGCTTTGCCAAACGAGAGGAGACGCTGCGTCAGGCAGCGGAACGACTATgc                                                                                                                                 >  1:347592/1‑149 (MQ=255)
                        gTCGCTGTGGCTGACGCGTGAGCACGGCGTTGCGACCATCCCGGTATCGGTGTTCTACGAGCAACCCATCCCCGAGCAACGCCTGGTACGCCTGTGCTTTGCCAAACGAGAGGAGACACTGCGTCAGGCAGCGGAACGACTATgc                                                                                                                                 >  7:31415/1‑145 (MQ=255)
                        gTCGCTGTGGCTGACGCGTGAGCACGGCGTTGCGACCATCCCGGTATCGGTGTTCTACCAGCAACCCATCCCCGAGCAACGCCTGGTACGCCTGTGCTTTGCCAAACGAGAGGAGACACTGCGTCAGGCAGCGGAACGACTATgc                                                                                                                                 <  8:31415/145‑1 (MQ=255)
                               tgGCTGACGCGTGAGCACGGCGTTGCGACCATCCCGGTATCGGTGTTCTACCAGCAACCCATCCCCGAGCAACGCCTGGTACGCCTGTGCTTTGCCAAACGAGAGGAGACGCTGCGTCAGGCAGCGGAACGACTATGCGCGATCTGAGt                                                                                                                      <  2:347592/149‑1 (MQ=255)
                                 gCTGACGCGTGAGCACGGCGTTGCGACCATCCCGGTATCGGTGTTCTACCAGCAACCCATCCCCGAGCAACGCCTGGTACGCCTGTGCTTTGCCAAACGAGAGGAGACGCTGCGTCAGGCAGCGGAACGACTATGCGCGATCTGa                                                                                                                        <  5:5729/145‑1 (MQ=255)
                                 gCTGACGCGTGAGCACGGCGTTGCGACCATCCCGGTATCGGTGTTCTACCAGCAACCCATCCCCGAGCAACGCCTGGTACGCCTGTGCTTTGCCAAACGAGAGGAGACGCTGCGTCAGGCAGCGGAACGACTATGCGCGATCTGa                                                                                                                        >  6:5729/1‑145 (MQ=255)
                                     aCGCGTGAGCACGGCGTTGCGACCATCCCGGTATCGGTGTTCTACCAGCAACCCATCCCCGAGCAACGCCTGGTACGCCTGTGCTTTGCCAAACGAGAGGAGACGCTGCGTCAGGCAGCGGAACGACTATGCGCGATCTGAGTACACTg                                                                                                                <  1:343602/149‑1 (MQ=255)
                                         gTGAGCACGGCGTTGCGACCATCCCGGTATCGGTGTTCTACCAGCAACCCATCCCCGAGCAACGCCTGGTACGCCTGTGCTTTGCCAAACGAGAGGAGACGCTGCGTCAGGCAGCGGAACGACTATGCGCGATCTGAGTACACTGCCg                                                                                                             >  1:99276/1‑148 (MQ=255)
                                                        cGACCATCCCGGTATCGGTGTTCTACCAGCAACCCATCCCCGAGCAACGCCTGGTACGCCTGTGCTTTGCCAAACGAGAGGAGACGCTGCGTCAGGCAGCGGAACGACTATGCGCGATCTGAGTACACTGCCGAACCTGAAAGTCGcc                                                                                              <  4:174145/148‑1 (MQ=255)
                                                            cATCCCGGTATCGGTGTTCTACCAGCAACCCATCCCCGAGCAACGCCTGGTACGCCTGTGCTTTGCCAACCGAGAGGAGACGCTGCGTCAGGCAGCGGAACGACTATGCGCGATCTGAGTACACTGCCGAACCTGAAAGTCGCCCTGGt                                                                                         <  6:291879/149‑1 (MQ=255)
                                                              tCCCGGTATCGGTGTTCTACCAGCAACCCATCCCCGAGCAACGCCTGGTACGCCTGTGCTTTGCCAAACGAGAGGAGACGCTGCGTCAGGCAGCGGAACGACTATGCGCGATCTGAGTACACTGCCGAACCTGAAAGTCGCCCTGGTg                                                                                        >  5:238432/1‑148 (MQ=255)
                                                                 cGGTATCGGTGTTCTACCAGCAACCCATCCCCGAGCAACGCCTGGTACGCCTGTGCTTTGCCAAACGAGAGGAGACGCTGCGTCAGGCAGCGGAACGACTATGCGCGATCTGAGTACACTGCCGAACCTGAAAGTCGCCCTGGTGCaaa                                                                                    >  5:146027/1‑149 (MQ=255)
                                                                    tATCGGTGTTCTACCAGCAACCCATCCCCGAGCAACGCCTGGTACGCCTGTGCTTTGCCAAACGAGAGGAGACGCTGCGTCAGGCAGCGGAACGACTATGCGCGATCTGAGTACACTGCCGAACCTGAAAGTCGCCCTGGTGCAAacca                                                                                 <  6:146027/149‑1 (MQ=255)
                                                                      tCGGTGTTCTACCAGCAACCCATCCCCGAGCAACGCCTGGTACGCCTGTGCTTTGCCAAACGAGAGGAGACGCTGCGTCAGGCAGCGGAACGACTATGCGCGATCTGAGTACACTGCCg                                                                                                             >  2:289172/1‑119 (MQ=255)
                                                                      tCGGTGTTCTACCAGCAACCCATCCCCGAGCAACGCCTGGTACGCCTGTGCTTTGCCAAACGAGAGGAGACGCTGCGTCAGGCAGCGGAACGACTATGCGCGATCTGAGTACACTGCCg                                                                                                             <  1:289172/119‑1 (MQ=255)
                                                                        ggTGTTCTACCAGCAACCCATCCCCGAGCAACGCCTGGTACGCCTGTGCTTTGCCAAACGAGAGGAGACGCTGCGTCAGGCAGCGGAACGACTATGCGCGATCTGAg                                                                                                                       <  3:335070/107‑1 (MQ=255)
                                                                        ggTGTTCTACCAGCAACCCATCCCCGAGCAACGCCTGGTACGCCTGTGCTTTGCCAAACGAGAGGAGACGCTGCGTCAGGCAGCGGAACGACTATGCGCGATCTGAg                                                                                                                       >  4:335070/1‑107 (MQ=255)
                                                                        ggTGTTCTACCAGCAACCCATCCCCGAGCAACGCCTGGTACGCCTGTGCTTTGCCAAACGAGAGGAGACGCTGCGTCAGGCAGCGGAACGACTATGCGCGATCTGAGTACACTGCCGAACCTGAAAGTCGCCCTGGTGCAAACCACCtt                                                                             >  6:342228/1‑149 (MQ=255)
                                                                        ggTGTTCTACCAGCAACCCATCCCCGAGCAACGCCTGGTACGCCTGTGCTTTGCCAAACGAGAGGAGACGCTGCCTGACGCAGCgtc                                                                                                                                           <  5:49909/87‑3 (MQ=255)
                                                                        ggTGTTCTACCAGCAACCCATCCCCGAGCAACGCCTGGTACGCCTGTGCTTTGCCAAACGAGAGGAGACGCTGCCTGACGCAGCgtc                                                                                                                                           >  6:49909/1‑85 (MQ=255)
                                                                          tgtTCTAACAGCAACCCATCCCGGAGTCAGGGCTGGTACGCCTGTGGTTTGCCAAACGAGAGGAGACGCTGCGTCAGGCAGCGGAACGACTATGCGCGATCTGAGTACACTGCCGAACCTGAAAGTCGCCCTGGTGCTAACCACCt                                                                              <  8:146366/146‑1 (MQ=255)
                                                                                 ccAGCAACCCATCCCCGAGCAACGCCTGGTACGCCTGTGCTTTGCCAAACGAGAGGAGACGCTGCGTCAGGCAGCGGAACGACTATGCGCGATCTGAGTACACTGCCGAACCTGAAAGTCGCCCTGGTGCAAACCACCTTGGCCTGGCa                                                                    >  2:307130/1‑149 (MQ=255)
                                                                                  cAGCAACCCATCCCCGAGCAACGCCTGGTACGCCTGTGCTTTGCCAAACGAGAGGAGACGCTGCGTCAGGCAGCGGAACGACTATGCGCGATCTGAGTACACTGCCGAACCTGa                                                                                                      >  3:64120/1‑114 (MQ=255)
                                                                                  cAGCAACCCATCCCCGAGCAACGCCTGGTACGCCTGTGCTTTGCCAAACGAGAGGAGACGCTGCGTCAGGCAGCGGAACGACTATGCGCGATCTGAGTACACTGCCGAACCTGa                                                                                                      <  4:64120/114‑1 (MQ=255)
                                                                                   aGCAACCCATCCCCGAGCAACGCCTGGTACGCCTGTGCTTTGCCAAACGAGAGGAGACGCTGCGTCAGGCAGCGGAACGACTATGCGCGATCTGAGTACACTGCCGAACCTGAAAGTCGCCCTGGTGCAAACCACCTTGGCCTGGCACg                                                                  >  2:11022/1‑149 (MQ=255)
                                                                                         ccATCCCCGAGCAACGCCTGGTACGCCTGTGCTTTGCCAAACGAGAGGAGACGCTGCGTCAGGCAGCGGAACGACTATGCGCGATCTGAGTACACTGCCGAACCTGAAAGTCGCCCTGGTGCAAaccac                                                                                >  7:141372/1‑129 (MQ=255)
                                                                                         ccATCCCCGAGCAACGCCTGGTACGCCTGTGCTTTGCCAAACGAGAGGAGACGCTGCGTCAGGCAGCGGAACGACTATGCGCGATCTGAGTACACTGCCGAACCTGAAAGTCGCCCTGGTGCAAaccac                                                                                <  8:141372/129‑1 (MQ=255)
                                                                                          cATCCCCGAGCAACGCCTGGTACGCCTGTGCTTTGCCAAACGAGAGGAGACGCTGCGTCAGGCAGCGGAACGACTATGCGCGATCTGAGTACACTGCCGAACCTGAAAGTCGCCCTGGTGc                                                                                       <  4:84030/121‑1 (MQ=255)
                                                                                          cATCCCCGAGCAACGCCTGGTACGCCTGTGCTTTGCCAAACGAGAGGAGACGCTGCGTCAGGCAGCGGAACGACTATGCGCGATCTGAGTACACTGCCGAACCTGAAAGTCGCCCTGGTGc                                                                                       >  3:84030/1‑121 (MQ=255)
                                                                                           aTCCCCGAGCAACGCCTGGTACGCCTGTGCTTTGCCAAACGAGAGGAGACGCTGCGTCAGGCAGCGGAACGACTATGCGCGATCTGAGTACACTGCCGAACCTGAAAGTCGCCCTGGTGCAAACCACCTTGGCCTGGCACGACCGCGAg                                                          <  5:327532/149‑1 (MQ=255)
                                                                                           aTCCCCGAGCAACGCCTGGTACGCCTGTGCTTTGCCAAACGAGAGGAGACGCTGCGTCAGGCAGCGGAACGACTATGCGCGATCTGAGTACACTGCCGAACCTGAAAGTCGCCCTGGTGCAAACCACCTTGGCCTGGCACGACCGCGAg                                                          <  5:342228/149‑1 (MQ=255)
                                                                                           aTCCCCGAGCAACGCCTGGTACGCCTGTGCTTTGCCAAACGAGAGGAGACGCTGCGTCAGGCAGCGGAACGACTATGCGCGATCTGAGTACACTGCCAAACCTGAAAGTCGCCCTGGTGCAAACCACCTTGGCCTGGCACGACCGCGAg                                                          >  7:260237/1‑149 (MQ=255)
                                                                                             ccccGAGCAACGCCTGGTACGCCTGTGCTTTGCCAAACGAGAGGAGACGCTGCGTCAGGCAGCGGAACGACTATGCGCGATCTGAGTACACTGCCGAACCTGAAAGTCGCCCTGGTGCAAACCACCTTGGCCTGGc                                                                     <  6:224085/136‑1 (MQ=255)
                                                                                             ccccGAGCAACGCCTGGTACGCCTGTGCTTTGCCAAACGAGAGGAGACGCTGCGTCAGGCAGCGGAACGACTATGCGCGATCTGAGTACACTGCCGAACCTGAAAGTCGCCCTGGTGCAAACCACCTTGGCCTGGc                                                                     >  5:224085/1‑136 (MQ=255)
                                                                                              cccGAGCAACGCCTGGTACGCCTGTGCTTTGCCAAACGAGAGGAGACGCTGCGTCAGGCAGCGGAACGACTATGCGCGATCTGAGTACACTGCCGAACCTGAAAGTCGCCCTGGTGCAAACCACCTTGGCCTGGCACGACCGCGAGGc                                                        >  2:291090/1‑148 (MQ=255)
                                                                                              cccGAGCAACGCCTGGTACGCCTGTGCTTTGCCAAACGAGAGGAGACGCTGCGTCAGGCAGCGGAACGACTATGCGCGATCTGAGTACACTGCCAAACCTGAAAGTCGCCCTGGTGCAAACCACCTTGGCCTGGCACGACCGCGAGGc                                                        <  8:260237/148‑1 (MQ=255)
                                                                                                      aCGCCTGGTACGCCTGTGCTTTGCCAAACGAGAGGAGACGCTGCGTCAGGCAGCGGAACGACTATGCGCGATCTGAGTACACTGCCGAACCTGAAAGTCGCCCTGGTGCAAACCACCTTGGCCTGGCACGACCGCGa                                                           <  4:88355/137‑1 (MQ=255)
                                                                                                      aCGCCTGGTACGCCTGTGCTTTGCCAAACGAGAGGAGACGCTGCGTCAGGCAGCGGAACGACTATGCGCGATCTGAGTACACTGCCGAACCTGAAAGTCGCCCTGGTGCAAACCACCTTGGCCTGGCACGACCGCGa                                                           >  3:88355/1‑137 (MQ=255)
                                                                                                                cGCCTGTGCTTTGCCAAACGAGAGGAGACGCTGCGTCAGGCAGCGGAACGACTATGCGCGATCTGAGTACACTGCCGAACCTGAAAGTCGCCCTGGTGCAAACCACCTTGGCCTGGCACGACCGCGAGGCCAACTACGCGCACTTCGAg                                     <  1:307130/149‑1 (MQ=255)
                                                                                                                 gCCTGTGCTTTGCCAAACGAGAGGAGACGCTGCGTCAGGCAGCGGAACGACTATGCGCGATCTGAGTACACTGCCGAACCTGAAAGTCGCCCTGGTGCAAACCACCTTGGCCTGGCACGACCGCGAGGCCAACTACGCGCACTTCGAgg                                    >  7:111506/1‑149 (MQ=255)
                                                                                                                 cggatctcTTTGCCAAACGAGAGGAGACGCTGCGTCAGGCAGCGGAACGACTATGCGCGATCTGAGTACACTGCCGAACCTGAAAGTCGCCCTGGTGCAAACCACCTTGGCCTGGCACGACCGCGAGGCCAACTACGCGCACTTCGAg                                     <  3:339091/141‑1 (MQ=255)
                                                                                                                     gtgCTTTGCCAAACGAGAGGAGACGCTGCGTCAGGCAGCGGAACGACTATGCGCGATCTGAGTACACTGCCGAACCTGAAAGTCGCCCTGGTGCAAACCACCTTGGCCTGGCACGACCGCGAGGCCAACTACGCGCACTTCGAGGtgct                                >  4:295680/1‑149 (MQ=255)
                                                                                                                         tttGCCAAACGAGAGGAGACGCTGCGTCAGGCAGCGGAACGACTATGCGCGATCTGAGTACACTGCCGAACCTGAAAGTCGCCCTGGTGCAAACCCCCATGGCCTGGCACGACCGCGAGGCCAACTACGCGCACTTCGac                                     >  4:339091/1‑139 (MQ=255)
                                                                                                                         tttGCCAAACGAGAGGAGACGCTGCGTCAGGCAGCGGAACGACTATGCGCGATCTGAGTACACTGCCGAACCTGAAAGTCGCCCTGGTGCAAACCACCTTGGCCTGGCACGACCGCGAGGCCAACTACGCGCACTTCGAGGTGctgctg                            >  7:18236/1‑149 (MQ=255)
                                                                                                                           tGCCAAACGAGAGGAGACGCTGCGTCAGGCAGCGGAACGACTATGCGCGATCTGAGTACACTGCCGAACCTGAAAGTCGCCCTGGTGCAAACCACCTTGGCCTGGCACGACCGCGAGGCCAACTACGCGCACTTCGAGGTGCTGCTgg                           <  2:99276/148‑1 (MQ=255)
                                                                                                                             ccAAACGAGAGGAGACGCTGCGTCAGGCAGCGGAACGACTATGCGCGATCTGAGTACACTGCCGAACCTGAAAGTCGCCCTGGTGCAAACCACCTTGGCCTGGCACGACCGCGAGGCCAACTACGCGCACTTCGAGGTGCTGCTGGa                          >  1:182361/1‑147 (MQ=255)
                                                                                                                             ccAAACGAGAGGAGACGCTGCGTCAGGCAGCGGAACGACTATGCGCGATCTGAGTACACTGCCGAACCTGAAAGTCGCCCTGGTGCAAACCACCTTGGCCTGGCACGACCGCGAGGCCAACTACGCGCACTTCGAGGTGCTGCTGGa                          <  2:182361/147‑1 (MQ=255)
                                                                                                                             ccAAACGAGAGGAGACGCTGCGTCAGGCAGCGGAACGACTATGCGCGATCTGAGTACACTGCCGAACCTGAAAGTCGCCCTGGTGCAAACCACCTTGGCCTGGCACGACCGCGAGGCCAACTACGCGCACTTCGAGGTGCTGCTGGAGc                        <  8:111506/149‑1 (MQ=255)
                                                                                                                               aaaCGAGAGGAGACGCTGCGTCAGGCAGCGGAACGACTATGCGCGATCTGAGTACACTGCCGAACCTGAAAGTCGCCCTGGTGCAAACCACCTTGGCCTGGCACGACCGCGAGGCCAACTACGCGCACTTCGAGGTGCTGCTGGAGCAg                      >  4:12809/1‑149 (MQ=255)
                                                                                                                                        gttccGCTGCCTGACGCAGCGGAACGACTATGCGCGATCTGAGTACACTGCCGAACCTGAAAGTCGCCCTGGTGCAAACCACCTTGGCCTGGCACGACCGCGAGGCCAACTACGCGCACTTCGAGGTGCTGCTGGAGCAGGTGGGCGAg             >  2:122281/5‑149 (MQ=255)
                                                                                                                                        gagaCGCTGCGTCAGGCAGCGGAACGACTATGCGCGATCTGAGTACACTGCCGAACCTGAAAGTCGCCCTGGTGCAAACCACCTTGGCCTGGCACGACCGCGAGGCCAACTACGCGCACTTCGAg                                     >  2:327165/1‑125 (MQ=255)
                                                                                                                                        gagaCGCTGCGTCAGGCAGCGGAACGACTATGCGCGATCTGAGTACACTGCCGAACCTGAAAGTCGCCCTGGTGCAAACCACCTTGGCCTGGCACGACCGCGAGGCCAACTACGCGCACTTCGAg                                     <  1:327165/125‑1 (MQ=255)
                                                                                                                                                  gTCAGGCAGCGGAACGACTATGCGCGATCTGAGTACACTGCCGAACCTGAAAGTCGCCCTGGTGCAAACCACCTTGGCCTGGCACGACCGCGAGGCCAACTACGCGCACTTCGAGGTGCTGCTGGAGCAGGTGGGCGAGGTCGACCTgg   <  1:291090/149‑1 (MQ=255)
                                                                                                                                                   tCAGGCAGCGGAACGACTATGCGCGATCTGAGTACACTGCCGAACCTGAAAGTCGCCCTGGTGCAAACCACCTTGGCCTGGCACGACCGCGAGGCCAACTACGCGCACTTCGAGGTGCTGCTGGAGCAGGTGGGCGAGGTCGACCTGGt  >  5:208067/1‑149 (MQ=255)
                                                                                                                                                    |                                                                                                                                                   
CCCGGACCTGAACGATGTCGACATGTCGCTGTGGCTGACGCGTGAGCACGGCGTTGCGACCATCCCGGTATCGGTGTTCTACCAGCAACCCATCCCCGAGCAACGCCTGGTACGCCTGTGCTTTGCCAAACGAGAGGAGACGCTGCGTCAGGCAGCGGAACGACTATGCGCGATCTGAGTACACTGCCGAACCTGAAAGTCGCCCTGGTGCAAACCACCTTGGCCTGGCACGACCGCGAGGCCAACTACGCGCACTTCGAGGTGCTGCTGGAGCAGGTGGGCGAGGTCGACCTGGT  >  NC_002947/993965‑994260

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑