Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 994,115 G→C 6.9% Q374H (CAG→CAC) ‡ PP_0858 → methionine/glutamine aminotransferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_002947994,1150GC 6.9% 177.8 / 4.2 58Q374H (CAG→CAC) ‡PP_0858methionine/glutamine aminotransferase
Reads supporting (aligned to +/- strand):  ref base G (27/27);  new base C (2/2);  total (29/29)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.78e-01

TGAACGATGTCGACATGTCGCTGTGGCTGACGCGTGAGCACGGCGTTGCGACCATCCCGGTATCGGTGTTCTACCAGCAACCCATCCCCGAGCAACGCCTGGTACGCCTGTGCTTTGCCAAACGAGAGGAGACGCTGCGTCAGGCAGCGGAACGACTATGCGCGATCTGAGTACACTGCCGAACCTGAAAGTCGCCCTGGTGCAAACCACCTTGGCCTGGCACGACCGCGAGGCCAACTACGCGCACTTCGAGGTGCTGCTGGAGCAGGTGGGCGAGGTCGACCTGGTG  >  NC_002947/993973‑994261
                                                                                                                                              |                                                                                                                                                  
tGAACGATGTCGACATGTCGCTGTGGCTGACGCGTGAGCACGGCGTTGCGACCATCCCGGTATCGGTGTTCTACCAGCAACCCATCCCCGAGCAACGCCTGGTACGCCTGTGCTTTGCCAAACGAGAGGAGACGCTGCGTCAGGCAGCg                                                                                                                                              >  3:174145/1‑149 (MQ=255)
    cGATGTCGACATGTCGCTGTGGCTGACGCGTGAGCACGGCGTTGCGACCATCCCGGTATCGGTGTTCTACCAGCAACCCATCCCCGAGCAACGCCTGGTACGCCTGTGCTTTGCCAAACGAGAGGAGACGCTGCCTGACGCAGCgtctc                                                                                                                                          <  4:78297/149‑5 (MQ=255)
         tCGACATGTCGCTGTGGCTGACGCGTGAGCACGGCGTTGCGACCATCCCGGTATCGGTGTTCTACCAGCAACCCATCCCCGAGCAACGCCTGGTACGCCTGTGCTTTGCCAAACGAGAGGAGACGCTGCGTCAGGCAGCGGAACGACt                                                                                                                                      <  8:316659/148‑1 (MQ=255)
            aCATGTCGCTGTGGCTGACGCGTGAGCACGGCGTTGCGACCATCCCGGTATCGGTGTTCTACCAGCAACCCATCCCCGAGCAACGCCTGGTACGCCTGTGCTTTGCCAAACGAGAGGAGACGCTGCGTCAGGCAGCGGAACGACTATgc                                                                                                                                  >  1:347592/1‑149 (MQ=255)
                gTCGCTGTGGCTGACGCGTGAGCACGGCGTTGCGACCATCCCGGTATCGGTGTTCTACGAGCAACCCATCCCCGAGCAACGCCTGGTACGCCTGTGCTTTGCCAAACGAGAGGAGACACTGCGTCAGGCAGCGGAACGACTATgc                                                                                                                                  >  7:31415/1‑145 (MQ=255)
                gTCGCTGTGGCTGACGCGTGAGCACGGCGTTGCGACCATCCCGGTATCGGTGTTCTACCAGCAACCCATCCCCGAGCAACGCCTGGTACGCCTGTGCTTTGCCAAACGAGAGGAGACACTGCGTCAGGCAGCGGAACGACTATgc                                                                                                                                  <  8:31415/145‑1 (MQ=255)
                       tgGCTGACGCGTGAGCACGGCGTTGCGACCATCCCGGTATCGGTGTTCTACCAGCAACCCATCCCCGAGCAACGCCTGGTACGCCTGTGCTTTGCCAAACGAGAGGAGACGCTGCGTCAGGCAGCGGAACGACTATGCGCGATCTGAGt                                                                                                                       <  2:347592/149‑1 (MQ=255)
                         gCTGACGCGTGAGCACGGCGTTGCGACCATCCCGGTATCGGTGTTCTACCAGCAACCCATCCCCGAGCAACGCCTGGTACGCCTGTGCTTTGCCAAACGAGAGGAGACGCTGCGTCAGGCAGCGGAACGACTATGCGCGATCTGa                                                                                                                         <  5:5729/145‑1 (MQ=255)
                         gCTGACGCGTGAGCACGGCGTTGCGACCATCCCGGTATCGGTGTTCTACCAGCAACCCATCCCCGAGCAACGCCTGGTACGCCTGTGCTTTGCCAAACGAGAGGAGACGCTGCGTCAGGCAGCGGAACGACTATGCGCGATCTGa                                                                                                                         >  6:5729/1‑145 (MQ=255)
                             aCGCGTGAGCACGGCGTTGCGACCATCCCGGTATCGGTGTTCTACCAGCAACCCATCCCCGAGCAACGCCTGGTACGCCTGTGCTTTGCCAAACGAGAGGAGACGCTGCGTCAGGCAGCGGAACGACTATGCGCGATCTGAGTACACTg                                                                                                                 <  1:343602/149‑1 (MQ=255)
                                 gTGAGCACGGCGTTGCGACCATCCCGGTATCGGTGTTCTACCAGCAACCCATCCCCGAGCAACGCCTGGTACGCCTGTGCTTTGCCAAACGAGAGGAGACGCTGCGTCAGGCAGCGGAACGACTATGCGCGATCTGAGTACACTGCCg                                                                                                              >  1:99276/1‑148 (MQ=255)
                                                cGACCATCCCGGTATCGGTGTTCTACCAGCAACCCATCCCCGAGCAACGCCTGGTACGCCTGTGCTTTGCCAAACGAGAGGAGACGCTGCGTCAGGCAGCGGAACGACTATGCGCGATCTGAGTACACTGCCGAACCTGAAAGTCGcc                                                                                               <  4:174145/148‑1 (MQ=255)
                                                    cATCCCGGTATCGGTGTTCTACCAGCAACCCATCCCCGAGCAACGCCTGGTACGCCTGTGCTTTGCCAACCGAGAGGAGACGCTGCGTCAGGCAGCGGAACGACTATGCGCGATCTGAGTACACTGCCGAACCTGAAAGTCGCCCTGGt                                                                                          <  6:291879/149‑1 (MQ=255)
                                                      tCCCGGTATCGGTGTTCTACCAGCAACCCATCCCCGAGCAACGCCTGGTACGCCTGTGCTTTGCCAAACGAGAGGAGACGCTGCGTCAGGCAGCGGAACGACTATGCGCGATCTGAGTACACTGCCGAACCTGAAAGTCGCCCTGGTg                                                                                         >  5:238432/1‑148 (MQ=255)
                                                         cGGTATCGGTGTTCTACCAGCAACCCATCCCCGAGCAACGCCTGGTACGCCTGTGCTTTGCCAAACGAGAGGAGACGCTGCGTCAGGCAGCGGAACGACTATGCGCGATCTGAGTACACTGCCGAACCTGAAAGTCGCCCTGGTGCaaa                                                                                     >  5:146027/1‑149 (MQ=255)
                                                            tATCGGTGTTCTACCAGCAACCCATCCCCGAGCAACGCCTGGTACGCCTGTGCTTTGCCAAACGAGAGGAGACGCTGCGTCAGGCAGCGGAACGACTATGCGCGATCTGAGTACACTGCCGAACCTGAAAGTCGCCCTGGTGCAAacca                                                                                  <  6:146027/149‑1 (MQ=255)
                                                              tCGGTGTTCTACCAGCAACCCATCCCCGAGCAACGCCTGGTACGCCTGTGCTTTGCCAAACGAGAGGAGACGCTGCGTCAGGCAGCGGAACGACTATGCGCGATCTGAGTACACTGCCg                                                                                                              >  2:289172/1‑119 (MQ=255)
                                                              tCGGTGTTCTACCAGCAACCCATCCCCGAGCAACGCCTGGTACGCCTGTGCTTTGCCAAACGAGAGGAGACGCTGCGTCAGGCAGCGGAACGACTATGCGCGATCTGAGTACACTGCCg                                                                                                              <  1:289172/119‑1 (MQ=255)
                                                                ggTGTTCTACCAGCAACCCATCCCCGAGCAACGCCTGGTACGCCTGTGCTTTGCCAAACGAGAGGAGACGCTGCGTCAGGCAGCGGAACGACTATGCGCGATCTGAg                                                                                                                        >  4:335070/1‑107 (MQ=255)
                                                                ggTGTTCTACCAGCAACCCATCCCCGAGCAACGCCTGGTACGCCTGTGCTTTGCCAAACGAGAGGAGACGCTGCGTCAGGCAGCGGAACGACTATGCGCGATCTGAg                                                                                                                        <  3:335070/107‑1 (MQ=255)
                                                                ggTGTTCTACCAGCAACCCATCCCCGAGCAACGCCTGGTACGCCTGTGCTTTGCCAAACGAGAGGAGACGCTGCGTCAGGCAGCGGAACGACTATGCGCGATCTGAGTACACTGCCGAACCTGAAAGTCGCCCTGGTGCAAACCACCtt                                                                              >  6:342228/1‑149 (MQ=255)
                                                                ggTGTTCTACCAGCAACCCATCCCCGAGCAACGCCTGGTACGCCTGTGCTTTGCCAAACGAGAGGAGACGCTGCCTGACGCAGCgtc                                                                                                                                            >  6:49909/1‑85 (MQ=255)
                                                                ggTGTTCTACCAGCAACCCATCCCCGAGCAACGCCTGGTACGCCTGTGCTTTGCCAAACGAGAGGAGACGCTGCCTGACGCAGCgtc                                                                                                                                            <  5:49909/87‑3 (MQ=255)
                                                                  tgtTCTAACAGCAACCCATCCCGGAGTCAGGGCTGGTACGCCTGTGGTTTGCCAAACGAGAGGAGACGCTGCGTCAGGCAGCGGAACGACTATGCGCGATCTGAGTACACTGCCGAACCTGAAAGTCGCCCTGGTGCTAACCACCt                                                                               <  8:146366/146‑1 (MQ=255)
                                                                         ccAGCAACCCATCCCCGAGCAACGCCTGGTACGCCTGTGCTTTGCCAAACGAGAGGAGACGCTGCGTCAGGCAGCGGAACGACTATGCGCGATCTGAGTACACTGCCGAACCTGAAAGTCGCCCTGGTGCAAACCACCTTGGCCTGGCa                                                                     >  2:307130/1‑149 (MQ=255)
                                                                          cAGCAACCCATCCCCGAGCAACGCCTGGTACGCCTGTGCTTTGCCAAACGAGAGGAGACGCTGCGTCAGGCAGCGGAACGACTATGCGCGATCTGAGTACACTGCCGAACCTGa                                                                                                       >  3:64120/1‑114 (MQ=255)
                                                                          cAGCAACCCATCCCCGAGCAACGCCTGGTACGCCTGTGCTTTGCCAAACGAGAGGAGACGCTGCGTCAGGCAGCGGAACGACTATGCGCGATCTGAGTACACTGCCGAACCTGa                                                                                                       <  4:64120/114‑1 (MQ=255)
                                                                           aGCAACCCATCCCCGAGCAACGCCTGGTACGCCTGTGCTTTGCCAAACGAGAGGAGACGCTGCGTCAGGCAGCGGAACGACTATGCGCGATCTGAGTACACTGCCGAACCTGAAAGTCGCCCTGGTGCAAACCACCTTGGCCTGGCACg                                                                   >  2:11022/1‑149 (MQ=255)
                                                                                 ccATCCCCGAGCAACGCCTGGTACGCCTGTGCTTTGCCAAACGAGAGGAGACGCTGCGTCAGGCAGCGGAACGACTATGCGCGATCTGAGTACACTGCCGAACCTGAAAGTCGCCCTGGTGCAAaccac                                                                                 <  8:141372/129‑1 (MQ=255)
                                                                                 ccATCCCCGAGCAACGCCTGGTACGCCTGTGCTTTGCCAAACGAGAGGAGACGCTGCGTCAGGCAGCGGAACGACTATGCGCGATCTGAGTACACTGCCGAACCTGAAAGTCGCCCTGGTGCAAaccac                                                                                 >  7:141372/1‑129 (MQ=255)
                                                                                  cATCCCCGAGCAACGCCTGGTACGCCTGTGCTTTGCCAAACGAGAGGAGACGCTGCGTCAGGCAGCGGAACGACTATGCGCGATCTGAGTACACTGCCGAACCTGAAAGTCGCCCTGGTGc                                                                                        >  3:84030/1‑121 (MQ=255)
                                                                                  cATCCCCGAGCAACGCCTGGTACGCCTGTGCTTTGCCAAACGAGAGGAGACGCTGCGTCAGGCAGCGGAACGACTATGCGCGATCTGAGTACACTGCCGAACCTGAAAGTCGCCCTGGTGc                                                                                        <  4:84030/121‑1 (MQ=255)
                                                                                   aTCCCCGAGCAACGCCTGGTACGCCTGTGCTTTGCCAAACGAGAGGAGACGCTGCGTCAGGCAGCGGAACGACTATGCGCGATCTGAGTACACTGCCGAACCTGAAAGTCGCCCTGGTGCAAACCACCTTGGCCTGGCACGACCGCGAg                                                           <  5:342228/149‑1 (MQ=255)
                                                                                   aTCCCCGAGCAACGCCTGGTACGCCTGTGCTTTGCCAAACGAGAGGAGACGCTGCGTCAGGCAGCGGAACGACTATGCGCGATCTGAGTACACTGCCGAACCTGAAAGTCGCCCTGGTGCAAACCACCTTGGCCTGGCACGACCGCGAg                                                           <  5:327532/149‑1 (MQ=255)
                                                                                   aTCCCCGAGCAACGCCTGGTACGCCTGTGCTTTGCCAAACGAGAGGAGACGCTGCGTCAGGCAGCGGAACGACTATGCGCGATCTGAGTACACTGCCAAACCTGAAAGTCGCCCTGGTGCAAACCACCTTGGCCTGGCACGACCGCGAg                                                           >  7:260237/1‑149 (MQ=255)
                                                                                     ccccGAGCAACGCCTGGTACGCCTGTGCTTTGCCAAACGAGAGGAGACGCTGCGTCAGGCAGCGGAACGACTATGCGCGATCTGAGTACACTGCCGAACCTGAAAGTCGCCCTGGTGCAAACCACCTTGGCCTGGc                                                                      <  6:224085/136‑1 (MQ=255)
                                                                                     ccccGAGCAACGCCTGGTACGCCTGTGCTTTGCCAAACGAGAGGAGACGCTGCGTCAGGCAGCGGAACGACTATGCGCGATCTGAGTACACTGCCGAACCTGAAAGTCGCCCTGGTGCAAACCACCTTGGCCTGGc                                                                      >  5:224085/1‑136 (MQ=255)
                                                                                      cccGAGCAACGCCTGGTACGCCTGTGCTTTGCCAAACGAGAGGAGACGCTGCGTCAGGCAGCGGAACGACTATGCGCGATCTGAGTACACTGCCGAACCTGAAAGTCGCCCTGGTGCAAACCACCTTGGCCTGGCACGACCGCGAGGc                                                         >  2:291090/1‑148 (MQ=255)
                                                                                      cccGAGCAACGCCTGGTACGCCTGTGCTTTGCCAAACGAGAGGAGACGCTGCGTCAGGCAGCGGAACGACTATGCGCGATCTGAGTACACTGCCAAACCTGAAAGTCGCCCTGGTGCAAACCACCTTGGCCTGGCACGACCGCGAGGc                                                         <  8:260237/148‑1 (MQ=255)
                                                                                              aCGCCTGGTACGCCTGTGCTTTGCCAAACGAGAGGAGACGCTGCGTCAGGCAGCGGAACGACTATGCGCGATCTGAGTACACTGCCGAACCTGAAAGTCGCCCTGGTGCAAACCACCTTGGCCTGGCACGACCGCGa                                                            <  4:88355/137‑1 (MQ=255)
                                                                                              aCGCCTGGTACGCCTGTGCTTTGCCAAACGAGAGGAGACGCTGCGTCAGGCAGCGGAACGACTATGCGCGATCTGAGTACACTGCCGAACCTGAAAGTCGCCCTGGTGCAAACCACCTTGGCCTGGCACGACCGCGa                                                            >  3:88355/1‑137 (MQ=255)
                                                                                                        cGCCTGTGCTTTGCCAAACGAGAGGAGACGCTGCGTCAGGCAGCGGAACGACTATGCGCGATCTGAGTACACTGCCGAACCTGAAAGTCGCCCTGGTGCAAACCACCTTGGCCTGGCACGACCGCGAGGCCAACTACGCGCACTTCGAg                                      <  1:307130/149‑1 (MQ=255)
                                                                                                         gCCTGTGCTTTGCCAAACGAGAGGAGACGCTGCGTCAGGCAGCGGAACGACTATGCGCGATCTGAGTACACTGCCGAACCTGAAAGTCGCCCTGGTGCAAACCACCTTGGCCTGGCACGACCGCGAGGCCAACTACGCGCACTTCGAgg                                     >  7:111506/1‑149 (MQ=255)
                                                                                                         cggatctcTTTGCCAAACGAGAGGAGACGCTGCGTCAGGCAGCGGAACGACTATGCGCGATCTGAGTACACTGCCGAACCTGAAAGTCGCCCTGGTGCAAACCACCTTGGCCTGGCACGACCGCGAGGCCAACTACGCGCACTTCGAg                                      <  3:339091/141‑1 (MQ=255)
                                                                                                             gtgCTTTGCCAAACGAGAGGAGACGCTGCGTCAGGCAGCGGAACGACTATGCGCGATCTGAGTACACTGCCGAACCTGAAAGTCGCCCTGGTGCAAACCACCTTGGCCTGGCACGACCGCGAGGCCAACTACGCGCACTTCGAGGtgct                                 >  4:295680/1‑149 (MQ=255)
                                                                                                                 tttGCCAAACGAGAGGAGACGCTGCGTCAGGCAGCGGAACGACTATGCGCGATCTGAGTACACTGCCGAACCTGAAAGTCGCCCTGGTGCAAACCCCCATGGCCTGGCACGACCGCGAGGCCAACTACGCGCACTTCGac                                      >  4:339091/1‑139 (MQ=255)
                                                                                                                 tttGCCAAACGAGAGGAGACGCTGCGTCAGGCAGCGGAACGACTATGCGCGATCTGAGTACACTGCCGAACCTGAAAGTCGCCCTGGTGCAAACCACCTTGGCCTGGCACGACCGCGAGGCCAACTACGCGCACTTCGAGGTGctgctg                             >  7:18236/1‑149 (MQ=255)
                                                                                                                   tGCCAAACGAGAGGAGACGCTGCGTCAGGCAGCGGAACGACTATGCGCGATCTGAGTACACTGCCGAACCTGAAAGTCGCCCTGGTGCAAACCACCTTGGCCTGGCACGACCGCGAGGCCAACTACGCGCACTTCGAGGTGCTGCTgg                            <  2:99276/148‑1 (MQ=255)
                                                                                                                     ccAAACGAGAGGAGACGCTGCGTCAGGCAGCGGAACGACTATGCGCGATCTGAGTACACTGCCGAACCTGAAAGTCGCCCTGGTGCAAACCACCTTGGCCTGGCACGACCGCGAGGCCAACTACGCGCACTTCGAGGTGCTGCTGGa                           >  1:182361/1‑147 (MQ=255)
                                                                                                                     ccAAACGAGAGGAGACGCTGCGTCAGGCAGCGGAACGACTATGCGCGATCTGAGTACACTGCCGAACCTGAAAGTCGCCCTGGTGCAAACCACCTTGGCCTGGCACGACCGCGAGGCCAACTACGCGCACTTCGAGGTGCTGCTGGa                           <  2:182361/147‑1 (MQ=255)
                                                                                                                     ccAAACGAGAGGAGACGCTGCGTCAGGCAGCGGAACGACTATGCGCGATCTGAGTACACTGCCGAACCTGAAAGTCGCCCTGGTGCAAACCACCTTGGCCTGGCACGACCGCGAGGCCAACTACGCGCACTTCGAGGTGCTGCTGGAGc                         <  8:111506/149‑1 (MQ=255)
                                                                                                                       aaaCGAGAGGAGACGCTGCGTCAGGCAGCGGAACGACTATGCGCGATCTGAGTACACTGCCGAACCTGAAAGTCGCCCTGGTGCAAACCACCTTGGCCTGGCACGACCGCGAGGCCAACTACGCGCACTTCGAGGTGCTGCTGGAGCAg                       >  4:12809/1‑149 (MQ=255)
                                                                                                                                gttccGCTGCCTGACGCAGCGGAACGACTATGCGCGATCTGAGTACACTGCCGAACCTGAAAGTCGCCCTGGTGCAAACCACCTTGGCCTGGCACGACCGCGAGGCCAACTACGCGCACTTCGAGGTGCTGCTGGAGCAGGTGGGCGAg              >  2:122281/5‑149 (MQ=255)
                                                                                                                                gagaCGCTGCGTCAGGCAGCGGAACGACTATGCGCGATCTGAGTACACTGCCGAACCTGAAAGTCGCCCTGGTGCAAACCACCTTGGCCTGGCACGACCGCGAGGCCAACTACGCGCACTTCGAg                                      >  2:327165/1‑125 (MQ=255)
                                                                                                                                gagaCGCTGCGTCAGGCAGCGGAACGACTATGCGCGATCTGAGTACACTGCCGAACCTGAAAGTCGCCCTGGTGCAAACCACCTTGGCCTGGCACGACCGCGAGGCCAACTACGCGCACTTCGAg                                      <  1:327165/125‑1 (MQ=255)
                                                                                                                                          gTCAGGCAGCGGAACGACTATGCGCGATCTGAGTACACTGCCGAACCTGAAAGTCGCCCTGGTGCAAACCACCTTGGCCTGGCACGACCGCGAGGCCAACTACGCGCACTTCGAGGTGCTGCTGGAGCAGGTGGGCGAGGTCGACCTgg    <  1:291090/149‑1 (MQ=255)
                                                                                                                                           tCAGGCAGCGGAACGACTATGCGCGATCTGAGTACACTGCCGAACCTGAAAGTCGCCCTGGTGCAAACCACCTTGGCCTGGCACGACCGCGAGGCCAACTACGCGCACTTCGAGGTGCTGCTGGAGCAGGTGGGCGAGGTCGACCTGGt   >  5:208067/1‑149 (MQ=255)
                                                                                                                                             aGGCAGCGGAACGACTATGCGCGATCTGAGTACACTGCCGAACCTGAAAGTCGCCCTGGTGCAAACCACCTTGGCCTGGCACGACCGCGAGGCCAACTACGCGCACTTCGAGGTGCTGCTGGAGCAGGTGGGCGAGGTCGACCTGGTg  <  8:18236/148‑1 (MQ=255)
                                                                                                                                              |                                                                                                                                                  
TGAACGATGTCGACATGTCGCTGTGGCTGACGCGTGAGCACGGCGTTGCGACCATCCCGGTATCGGTGTTCTACCAGCAACCCATCCCCGAGCAACGCCTGGTACGCCTGTGCTTTGCCAAACGAGAGGAGACGCTGCGTCAGGCAGCGGAACGACTATGCGCGATCTGAGTACACTGCCGAACCTGAAAGTCGCCCTGGTGCAAACCACCTTGGCCTGGCACGACCGCGAGGCCAACTACGCGCACTTCGAGGTGCTGCTGGAGCAGGTGGGCGAGGTCGACCTGGTG  >  NC_002947/993973‑994261

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑