Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 997,751 G→C 8.2% intergenic (‑156/+76) PP_0860 ← / ← PP_0861 sulfite reductase flavoprotein subunit/outer membrane ferric siderophore receptor

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_002947997,7510GC 8.2% 188.1 / 7.0 61intergenic (‑156/+76)PP_0860/PP_0861sulfite reductase flavoprotein subunit/outer membrane ferric siderophore receptor
Reads supporting (aligned to +/- strand):  ref base G (30/26);  new base C (2/3);  total (32/29)
Fisher's exact test for biased strand distribution p-value = 6.62e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.19e-01

TCACCTTGAGTTGCTGCCGCGACCCGCGCGGCGTGCATTATGCCTTGATACACAAAAGCCCCGCTCACGGGAATGAACGGGGCCGGGAGGGCTGCTTTTCGTGTTGTCTGTGCGGGCCTCTTCGCGGCTAAAGCCGCTCCTGCACCGGCAACGCAGGAGCGGCTTCAGCCGCGAAAGGGCCGGCACAGGCGCAGCCCTTCAACAGATCAACCCAACCCTGACATCAGAAGTGCACGCTGGTGGTCAACAGTGCCGTGCGCCCGGCCGCCTGGTTGGCGAAGTGGGTAGAGAAGG  >  NC_002947/997604‑997897
                                                                                                                                                   |                                                                                                                                                  
tcACCTTGAGTTGCTGCCGCGACCCGCGCGGCGTGCATTATGCCTTGATACACAAAAGCCCCGCTCACGGGAATGAACGGGGCCGGGAGGGCTGCTTTTCGTGTTGTCTGTGCGGGCCTCTTCGCGGCTAAAGCCGCTCCTGCACCgg                                                                                                                                                    >  4:92989/1‑148 (MQ=255)
  aCCTTGAGTTGCTGCCGCGACCCGCGCGGCGTGCATTATGCCTTGATACACAAAAGCCCCGCTCACGGGAATGACCGGGGCCGGGAGGGCTGCTTTTCGTGTTGTCTGTGCTGGCCTCTTCGCGGCTAAAGCCGCTCCTGCACCGGCaa                                                                                                                                                 >  7:164830/1‑149 (MQ=255)
       gAGTTGCTGCCGCGACCCGCGCGGCGTGCATTATGCCTTGATACACAAAAGCCCCGCTCACGGGAATGAACGGGGCCGGGAGGGCTGCTTTTCGTGTTGTCTGTGCGGGCCTCTTCGCGGCTAAAGCCGCTCCTGCACCGGCAACGCa                                                                                                                                             <  6:158074/148‑1 (MQ=255)
       gAGTTGCTGCCGCGACCCGCGCGGCGTGCATTATGCCTTGATACACAAAAGCCCCGCTCACGGGAATGAACGGGGCCGGGAGGGCTGCTTTTCGTGTTGTCTGTGCGGGCCTCTTCGCGGCTAAAGCCGCTCCTGCACCGGCAACGCa                                                                                                                                             <  8:19974/148‑1 (MQ=255)
             ctgcCGCGAGCCGCGGGGCGTGCATTATGCCTTGATACACATAAGCCCCTCTCACGTGAATGAACGGGGCCGGGATGGCTGCTTTTCGTGTTGTCTGTGCGGGCCTCTTCTCTGCTAAAGCCGATCCTGCACCGGCAACGCAGGAGCg                                                                                                                                       <  8:164830/148‑1 (MQ=255)
                ccGCGACCCGCGCGGCGTGCATTATGCCTTGATACACAAAAGCCCCGCTCACGGGAATGAACGGGGCCGGGAGGGCTGCTTTTCGTGTTGTCTGTGCGGGCCTCTTCGCGGCTAAAGCCGCTCCTGCACCGGCAACGCAg                                                                                                                                            >  7:124933/1‑140 (MQ=255)
                ccGCGACCCGCGCGGCGTGCATTATGCCTTGATACACAAAAGCCCCGCTCACGGGAATGAACGGGGCCGGGAGGGCTGCTTTTCGTGTTGTCTGTGCGGGCCTCTTCGCGGCTAAAGCCGCTCCTGCACCGGCAACGCAg                                                                                                                                            <  8:124933/140‑1 (MQ=255)
                       ccGCGCGGCGTGCATTATGCCTTGATACACAAAAGCCCCGCTCACGGGAATGAACGGGGCCGGGAGGGCTGCTTTTCGTGTTGTCTGTGCGGGCCTCTTCGCGGCTAAAGCCGCTCCTGCACCGGCAACGCAGGAGCGGCTTCAGCcgc                                                                                                                            >  5:300927/1‑149 (MQ=255)
                         gcgcgGCGTGCATTATGCCTTGATACACAAAAGCCCCGCTCACGGGAATGAACGGGGCCGGGAGGGCTGCTTTTCGTGTTGTCTGTGCGGGCCTCTTCGCGGCTAAAGCCGCTCCTGCACCGGCAACGCAGGAGCGGCTTCAGCcgcg                                                                                                                           >  2:125175/1‑148 (MQ=255)
                          cgcgGCGTGCATTATGCCTTGATACACAAAAGCCCCGCTCACGGGAATGAACGGGGCCGGGAGGGCTGCTTTTCGTGTTGTCTGTGCGGGCCTCTTCGCGGCTAAAGCCGCTCCTGCACCGGCAACGCAGGAGCGGCTTCAGCCGCGaa                                                                                                                         <  5:84274/149‑1 (MQ=255)
                                gTGCATTATGCCTTGATACACAAAAGCCCCGCTCACGGGAATGAACGGGGCCGGGAGGGCTGCTTTTCGTGTTGTCTGTGCGGGCCTCTTCGCGGCTAAAGCCGCTCCTGCACCGGCAACGCAGGAGCGGCTTCAGCCGCGAAAGGGcc                                                                                                                   <  7:66013/149‑1 (MQ=255)
                                 tGCATTATGCCTTGATACACAAAAGCCCCGCTCACGGGAATGAACGGGGCCGGGAGGGCTGCTTTTCGTGTTGTCTGTGCGGGCCTCTTCGCGGCTAAAGCCGCTCCTGCACCGGCAACGCAGGAGCGGCTTCAGCCGCGAAAGGGCCg                                                                                                                  <  3:92989/149‑1 (MQ=255)
                                    aTTATGCCTTGATACACAAAAGCCCCGCTCACGGGAATGAACGGGGCCGGGAGGGCTGCTTTTCGTGTTGTCTGTGCCGGCCCTTTCGCGGCTGAAGCCGCTCCTGCGTTGCCGGTGCAGGAGCGGCTTTAGCCGCGAAGAGGCCCGc                                                                                                                <  6:305632/148‑1 (MQ=17)
                                           cTTGATACACAAAAGCCCCGCTCACGGGAATGAACGGGGCCGGGAGGGCTGCTTTTCGTGTTGTCTGTGCGGGCCTCTTCGCGGCTAAAGCCGCTCCTGCACCGGCAACGCAGGAGCGGCTTCAGCCGCGAAAGGGCCGGCACAGgcgc                                                                                                        >  4:37695/1‑149 (MQ=255)
                                           cTTGATACACAAAAGCCCCGCTCACGGGAATGAACGGGGCCGGGAGGGCTGCTTTTCGTGTTGTCTGTGCGGGCCTCTTCGCGGCTAAAGCCGCTCCTGCACCGGCAACGCAGGAGCGGCTTCAGCCGCGAAAGGGCCGGCACAGgcg                                                                                                         >  8:252373/1‑148 (MQ=255)
                                           cTTGATACACAAAAGCCCCGCTCACGGGAATGAACGGGGCCGGGAGGGCTGCTTTTCGTGTTGTCTGTGCGGGCCTCTTCGCGGCTAAAGCCGCTCCTGCACCGGCAACGCAGGAGCGGCTTCAGCCGCGAAAGGGCCGGCACAGgcg                                                                                                         >  4:310431/1‑148 (MQ=255)
                                              gATACACAAAAGCCCCGCTCACGGGAATGAACGGGGCCGGGAGGGCTGCTTTTCGTGTTGTCTGTGCGGGCCTCTTCGCGGCTAAAGCCGCTCCTGCACCGGCAACGCAGGAGc                                                                                                                                        <  4:63213/114‑1 (MQ=255)
                                              gATACACAAAAGCCCCGCTCACGGGAATGAACGGGGCCGGGAGGGCTGCGTTTCGTGTTGTCTGTGCGGGCCTCTTCGCGGCTAAAGCCGCTCCTGCACCGGCAACGCAGGAGCGGCTTCAGCCGCGAAAGGGCCGGCACAGGCGCa                                                                                                       <  1:125175/147‑1 (MQ=255)
                                                tACACAAAAGCCCCGCTCACGGGAATGAACGGGGCCGGGAGGGCTGCTTTTCGTGTTGTCTGTGCGGGCCTCTTCGCGGCTAAAGCCGCTCCTGCACCGGCAACGCAGGAGCGGCTTCAGCcg                                                                                                                             >  7:264632/1‑123 (MQ=255)
                                                tACACAAAAGCCCCGCTCACGGGAATGAACGGGGCCGGGAGGGCTGCTTTTCGTGTTGTCTGTGCGGGCCTCTTCGCGGCTAAAGCCGCTCCTGCACCGGCAACGCAGGAGCGGCTTCAGCcg                                                                                                                             <  8:264632/123‑1 (MQ=255)
                                                  cacaAAAGCCCCGCTCACGGGAATGAACGGGGCCGGGAGGGCTGCTTTTCGTGTTGTCTGTGCGGGCCTCTTCGCGGCTGAAGCCGCTCCTGCGTTGCCGGTGCAGGAGCGGCTTTAGCCGCGaa                                                                                                                         <  7:236702/125‑1 (MQ=21)
                                                  cacaAAAGCCCCGCTCACGGGAATGAACGGGGCCGGGAGGGCTGCTTTTCGTGTTGTCTGTGCGGGCCTCTTCGCGGCTGAAGCCGCTCCTGCGTTGCCGGTGCAGGAGCGGCTTTAGCCGCGaa                                                                                                                         >  8:236702/1‑125 (MQ=21)
                                                   acaAAAGCCCCGCTCACGGGAATGAACGGGGCCGGGAGGGCTGCTTTTCGTGTTGTCTGTGCGGGCCTCTTCGCGGCTAAAGCCGCTCCTGCACCGGCAACGCAGGAGCGGCTTCAGCCGCGAAAGGGCCGGCACAGGCGCAGCCCtt                                                                                                 >  1:344446/1‑148 (MQ=255)
                                                    caAAAGCCCCGCTCACGGGAATGAACGGGGCCGGGAGGGCTGCTTTTCGTGTTGTCTGTGCGGGCCTCTTCGCGGCTAAAGCCGCTCCTGCACCGGCAACGCAGGAGCGGCTTCAGCCGCGAAAGGGCCGGCACAGgcgc                                                                                                        >  8:104815/1‑140 (MQ=255)
                                                    caAAAGCCCCGCTCACGGGAATGAACGGGGCCGGGAGGGCTGCTTTTCGTGTTGTCTGTGCGGGCCTCTTCGCGGCTAAAGCCGCTCCTGCACCGGCAACGCAGGAGCGGCTTCAGCCGCGAAAGGGCCGGCACAGgcgc                                                                                                        <  7:104815/140‑1 (MQ=255)
                                                    caAAAGCCCCGCTCACGGGAATGAACGGGGCCGGGAGGGCTGCTTTTCGTGTTGTCTGTGCGGGCCTCTTCGCGGCTAAAGCCGCTCCTGCACCGGCAACGCAGGAGCGGCTTCAGCCGCGAAAGGGCCGGCACAGGCGCAGCCCTTc                                                                                                >  4:135118/1‑148 (MQ=255)
                                                     aaaaGCCCCGCTCACGGGAATGAACGGGGCCGGGAGGGCTGCTTTTCGTGTTGTCTGTGCGGGCCTCTTCGCGGCTAAAGCCGCTCCTGCACCGGCAACGCAGGAGCGGCTTCAGCCGCGAAAGGGCCGGCACAGGCGCAGCCCTTCaa                                                                                              >  1:136330/1‑149 (MQ=255)
                                                      aaaGCCCCGCTCACGGGAATGAACGGGGCCGGGAGGGCTGCTTTTCGTGTTGTCTGTGCGGGCCTCTTCGCGGCTAAAGCCGCTCCTGCACCGGCAACGCAGGAGCGGCTTCAGCCGCGAAAGGGCCGGcac                                                                                                              >  8:118421/1‑132 (MQ=255)
                                                      aaaGCCCCGCTCACGGGAATGAACGGGGCCGGGAGGGCTGCTTTTCGTGTTGTCTGTGCGGGCCTCTTCGCGGCTAAAGCCGCTCCTGCACCGGCAACGCAGGAGCGGCTTCAGCCGCGAAAGGGCCGGcac                                                                                                              <  7:118421/132‑1 (MQ=255)
                                                        aGCCCCGCTCACGGGAATGAACGGGGCCGGGAGGGCTGCTTTTCGTGTTGTCTGTGCGGGCCTCTTCGCGGCTAAAGCCGCTCCTGCACCGGCAACGCAGGAGCGGCTTCAGCCGCGAAAGGGCCGGCACAGGCGCAGCCCTTCAACAg                                                                                           <  2:344446/149‑1 (MQ=255)
                                                         gCCCCGCTCACGGGAATGAACGGGGCCGGGAGGGCTGCTTTTCGTGTTGTCTGTGCGGGCCTCTTCGCGGCTAAAGCCGCTCCTGCACCGGCAACGCAGGAGCGGCTTCAGCCGCGAAAGGGCCGGCACAGGCGCAGCCCTTCAACAGa                                                                                          <  6:300927/149‑1 (MQ=255)
                                                          ccccGCTCACGGGAATGAACGGGGCCGGGAGGGCTGCTTTTCGTGTTGTCTGTGCGGGCCTCTTCGCGGCTAAAGCCGCTCCTGCACCGGCAACGCAGGAGCGGCTTCAGCCGCGAAAGCGCCGGCACACGCGCAGCCCATCAACAGa                                                                                          >  4:278624/1‑148 (MQ=255)
                                                             cGCTCACGGGAATGAACGGGGCCGGGAGGGCTGCTTTTCGTGTTGTCTGTGCGGGCCTCTTCGCGGCTAAAACCCCAACAGCACCCGCAACCCAAGACCCGCGTCAGCCGCCAAATGGCCCGCACACGCGCACCCCATCAAAAGaaaa                                                                                       >  2:175120/1‑145 (MQ=255)
                                                              gCTCACGGGAATGAACGGGGCCGGGAGGGCTGCTTTTCGTGTTGTCTGTGCGGGCCTCTTCGCGGCTAAAGCCGCTCCTGCACCGGCAACGCAGGAGCGGCTTCAGCCGCGAAAGGGCCGGCACAGGCGCAGCCCTTCAACAGATcaac                                                                                     <  3:135118/149‑1 (MQ=255)
                                                                tCACGGGAATGAACGGGGCCGGGAGGGCTGCTTTTCGTGTTGTCTGTGCGGGCCTCTTCGCGGCTAAAGCCGCTCCTGCACCGGCAACGCAGGAGCGGCTTCAGCCGCGAAAGGGCCCGCACAGGCGCAGCCCTTCAACAGATcaccc                                                                                    >  8:41299/1‑148 (MQ=255)
                                                                    gggAATGAACGGGGCCGGGAGGGCTGCTTTTCGTGTTGTCTGTGCGGGCCTCTTCGCGGCTAAAGCCGCTCCTGCACCGGCAACGCAGGAGCGGCTTCAGCCGCGAAAGGGCCGGCACAGGCGCAGCCCTTCAACAGATcaacccaacc                                                                               >  6:276653/1‑149 (MQ=255)
                                                                                ggCCGGGAGGGCTGCTTTTCGTGTTGTCTGTGCGGGCCTCTTCGCGGCTAAAGCCGCTCCTGCACCGGCAACGCAGGAGCGGCTTCAGCCGCGAAAGGGCCGGCACAGGCGCAGCCCTTCAACAGATCAACCCAACCCTGACATCAGaa                                                                   <  3:278624/149‑1 (MQ=255)
                                                                                ggCCGGGAGGGCTGCTTTTCGTGTTGTCTGTGCGGGCCTCTTCGCGGCTAAAGCCGCTCCTGCACCGGCAACGCAGGAGCGGCTTCAGCCGCGAAAGGGCCGGCACAGGCGCAGCCCTTCAACAGATCAACCCAACCCTGACATCAGaa                                                                   <  3:37695/149‑1 (MQ=255)
                                                                                ggCCGGGAGGGCTGCTTTTCGTGTTGTCTGTGCGGGCCTCTTCGCGGCTAAAGCCGCTCCTGCACCGGCAACGCAGGAGCGGCTTCAGCCGCGAAAGGGCCGGCACAGGCGCAGCCCTTCAACAGATCAACCCAACCCTGACATCAGaa                                                                   <  7:252373/149‑1 (MQ=255)
                                                                                              ctTTTCGTGTTGTCTGTGCGGGCCTCTTCGCGGCTAAAGCCGCTCCTGCTCCGGCAACGCAGGAGCGGCTTCAGCCGCGAATGGGCCGGCAGAGGCGCAGCCCTTCTACAGATCAACGCAACCCTGACATCAGAAGTGCACGCtggtg                                                      <  7:41299/148‑1 (MQ=255)
                                                                                                 ttCGTGTTGTCTGTGCGGGCCTCTTCGCGGCTAAAGCCGCTCCTGCACCGGCAACGCAGGAGCGGCTTCAGCCGCGAAAGGGCCGGCACAGGCGCAGCCCTTCAACAGATCAACCCAACCCTGACATCAGAAGTGCACGCTggtggt                                                    <  5:276653/147‑1 (MQ=255)
                                                                                                    gtgtTGTCTGTGCGGGCCTCTTCGCGGCTAAAGCCGCTCCTGCACCGGCAACGCAGGAGCGGCTTCAGCCGCGAAAGGGCCGGCACAGGCGCAGCCCTTCAACAGATCAACCCAACCCTGACATCAGAAGTGCACGCTGGTGGTCaa                                                 >  1:209238/1‑147 (MQ=255)
                                                                                                          tCTGTGCGGGCCTCTTCGCGGCTAAAGCCGCTCCTGCACCGGCAACGCAGGAGCGGCTTCAGCCGCGAAAGGGCCGGCACAGGCGCAGCCCTTCAACAGATCAACCCAACCCTGACATCAGAAGTGCACGCTGGTGGTCAACAGTGCCg                                         >  1:296333/1‑149 (MQ=255)
                                                                                                               gCGGGCCTCTTCGCGGCTAAAGCCGCTCCTGCACCGGCAACGCAGGAGCGGCTTCAGCCGCGAAAGGGCCGGCACAGGCGCAGCCCTTCAACAGATCAACCCAACCCTGACATCAGAAGTGCACGCTGGTGGTCAACAGTGCCGTgcgc                                    >  5:152373/1‑149 (MQ=255)
                                                                                                                          cgcgGCTAAAGCCGCTCCTGCACCGGCAACGCAGGAGCGGCTTCAGCCGCGAAAGGGCCGGCACAGGCGCAGCCCTTCAACAGATCAACCCAACCCTGACATCAGAAGTGCACGCTGGTGGTCAACAGTGCCGTGCGCCCGgccgc                            <  2:209238/146‑1 (MQ=255)
                                                                                                                           gcgGCTAAAGCCGCTCCTGCACCGGCAACGCAGGAGCGGCTTCAGCCGCGAAAGGGCCGGCACAGGCGCAGCCCTTCAACAGATCAACCCAACCCTGACATCAGAAGTGCACGCTGGTGGTCAACAGTGCCGTgc                                      <  3:252098/135‑1 (MQ=255)
                                                                                                                           gcgGCTAAAGCCGCTCCTGCACCGGCAACGCAGGAGCGGCTTCAGCCGCGAAAGGGCCGGCACAGGCGCAGCCCTTCAACAGATCAACCCAACCCTGACATCAGAAGTGCACGCTGGTGGTCAACAGTGCCGTgc                                      >  4:252098/1‑135 (MQ=255)
                                                                                                                              gCTAAAGCCGCTCCTGCACCGGCAACGCAGGAGCGGCTTCAGCCGCGAAAGGGCCGGCACAGGCGCAGCCCTTCAACAGATCAACCCAACCCTGACATCAGAAGTGCACGCTGGTGGTCAACAGTGCCGTGCGCCCGGCCGCCTGGTTg                     <  3:310431/149‑1 (MQ=255)
                                                                                                                                tAAAGCCGCTCCTGCACCGGCAACGCAGGAGCGGCTTCAGCCGCGAAAGGGCCGGCACCGGCGCCGCCCTTCAACAGATCACCCCAACCCGGCCATCAGAAGTGCCCGCTGGTGGTCACCAGTGCCGTGCGCCCCGCCCCCGGGTTGGc                   >  7:199337/1‑149 (MQ=255)
                                                                                                                                tAAAGCCGCTCCTGCACCGGCAACGCAGGAGCGGCTTCAGCCGCGAAAGGGCCGGCACAGGCGCAGCCCTTCAACAGATCAACCCAACCCTGACATCAGAAGTGCACGCTGGTGGTCAACAGTGCCGTGCGCCCGGCCGCCTGGTTGGc                   >  4:89333/1‑149 (MQ=255)
                                                                                                                                  aaGCCGCTCCTGCGTTGCCGGTGCAGGAGCGGCTTCAGCCGCGAAAGGGCCGGCACAGGCGCAGCCCTTCAACAGATCAACCCAACCCTGACATCAGAAGTGCACGCTGGTGGTCAACAGTGCCGTGCGCCCGgccgcc                           <  5:37522/139‑1 (MQ=37)
                                                                                                                                  aaGCCGCTCCTGCGTTGCCGGTGCAGGAGCGGCTTCAGCCGCGAAAGGGCCGGCACAGGCGCAGCCCTTCAACAGATCAACCCAACCCTGACATCAGAAGTGCACGCTGGTGGTCAACAGTGCCGTGCGCCCGgccgcc                           >  6:37522/1‑139 (MQ=37)
                                                                                                                                    gCCGCTCCTGCACCGGCAACGCAGGAGCGGCTTCAGCCGCGAAAGGGCCGGCACAGGCGCAGCCCTTCAACAGATCAACCCAACCCTGACATCAGAAGTGCACGCTGGTGGTCAACAGTGCCGTGCGCCCGGCCGCCTGGTTGGCGAAg               >  1:80420/1‑149 (MQ=255)
                                                                                                                                     ccGCTCCTGCACCGGCAACGCAGGAGCGGCTTCAGCCGCGAAAGGGCCGGCACAGGCGCAGCCCTTCAACAGATCAACCCAACCCTGACATCAGAAGTGCACGCTGGTGGTCAACAGTGCCGTGCGCCCGGCCGCCTGGTTGGCGAAGt              <  2:296333/149‑1 (MQ=255)
                                                                                                                                         tCCTGCACCGGCAACGCAGGAGCGGCTTCAGCCGCGAAAGGGCCGGCACAGGCGCAGCCCTTCAACAGATCAACCCAACCCTGACATCAGAAGTGCACGCTGGTGGTCAACAGTGCCGTGCGCCCGGCCGCCTGGTTGGCGAAGTggg           >  8:231587/1‑148 (MQ=255)
                                                                                                                                           cTGCACCGGCAACGCAGGAGCGGCTTCAGCCGCGAAAGGGCCGGCACAGGCGCAGCCCTTCAACAGATCAACCCAACCCTGACATCAGAAGTGCACGCTGGTGGTCAACAGTGCCGTGCGCCCGGCCGCCTGGTTGGCGAAGTggg           >  3:146677/1‑146 (MQ=255)
                                                                                                                                           cTGCACCGGCAACGCAGGAGCGGCTTCAGCCGCGAAAGGGCCGGCACAGGCGCAGCCCTTCAACAGATCAACCCAACCCTGACATCAGAAGTGCACGCTGGTGGTCAACAGTGCCGTGCGCCAGGCCGCCTGGTTGGCGAAGTggg           >  3:148587/1‑146 (MQ=255)
                                                                                                                                            tGCACCGGCAACGCAGGAGCGGCTTCAGCCGCGAAAGGGCCGGCACAGGCGCAGCCCTTCAACAGATCAACCCAACCCTGACATCAGAAGTGCACGCTGGTGGTCAACAGTGCCGTGCGCCCGGCCGCCTGGTTGGCGAAGTGGGt          >  5:198168/1‑146 (MQ=255)
                                                                                                                                            tGCACCGGCAACGCAGGAGCGGCTTCAGCCGCGAAAGGGCCGGCACAGGCGCAGCCCTTCAACAGATCAACCCAACCCTGACATCAGAAGTGCACGCTGGTGGTCAACAGTGCCGTGCGCCCGGCCGCCTGGTTGGCGAAGTGGGt          <  6:198168/146‑1 (MQ=255)
                                                                                                                                                 cGGCAACGCAGGAGCGGCTTCAGCCGCGAAAGGGCCGGCACAGGCGCAGCCCTTCAACAGATCAACCCAACCCTGACATCAGAAGTGCACGCTGGTGGTCAACAGTGCCGTGCGCCCGGCCGCCTg                         <  1:166069/126‑1 (MQ=255)
                                                                                                                                                 cGGCAACGCAGGAGCGGCTTCAGCCGCGAAAGGGCCGGCACAGGCGCAGCCCTTCAACAGATCAACCCAACCCTGACATCAGAAGTGCACGCTGGTGGTCAACAGTGCCGTGCGCCCGGCCGCCTg                         >  2:166069/1‑126 (MQ=255)
                                                                                                                                                 cGGCAACGCAGGAGCGGCTTCAGCCGCGAAAGGGCCGGCACAGGCGCAGCCCTTCAACAGATCAACCCAACCCTGACATCAGAAGTGCACGCTGGTGGTCAACAGTGCCGTGCGCCCGGCCGCCTGGTTGGCGAAGTGGGTAGAGAAgg  >  4:63236/1‑149 (MQ=255)
                                                                                                                                                  ggCAACGCAGGAGCGGCTTCAGCCGCGAAAGGGCCGGCACAGGCGCAGCCCTTCAACAGATCAACCCAACCCTGACATCAGAAGTGCACGCTGGTGGTCAACAGTGCCGTGCGCCCg                                 <  6:338516/117‑1 (MQ=255)
                                                                                                                                                  ggCAACGCAGGAGCGGCTTCAGCCGCGAAAGGGCCGGCACAGGCGCAGCCCTTCAACAGATCAACCCAACCCTGACATCAGAAGTGCACGCTGGTGGTCAACAGTGCCGTGCGCCCg                                 >  5:338516/1‑117 (MQ=255)
                                                                                                                                                   |                                                                                                                                                  
TCACCTTGAGTTGCTGCCGCGACCCGCGCGGCGTGCATTATGCCTTGATACACAAAAGCCCCGCTCACGGGAATGAACGGGGCCGGGAGGGCTGCTTTTCGTGTTGTCTGTGCGGGCCTCTTCGCGGCTAAAGCCGCTCCTGCACCGGCAACGCAGGAGCGGCTTCAGCCGCGAAAGGGCCGGCACAGGCGCAGCCCTTCAACAGATCAACCCAACCCTGACATCAGAAGTGCACGCTGGTGGTCAACAGTGCCGTGCGCCCGGCCGCCTGGTTGGCGAAGTGGGTAGAGAAGG  >  NC_002947/997604‑997897

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑