Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 1,001,071 G→A 8.7% L16L (TTG→TTA)  PP_0863 → hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029471,001,0710GA 8.7% 196.2 / 6.3 69L16L (TTG→TTA) PP_0863hypothetical protein
Reads supporting (aligned to +/- strand):  ref base G (34/29);  new base A (3/3);  total (37/32)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.38e-01

CAGCAACTGACTGCCGATGTGCCGGACCATCCTTCGCTTTTGCAGCTGACGGGCACCTACCATAATTTGCTGCGCCGCTGGGCTGAGGTCTGAGCCGTGTCCTACCTGCTGCGACGTGAGGAAGTGGTGGATGTGGCCGGCTTGCAGGCCATGCTCGAGGAAACTCCCGGCAAGGCCGCCCAGGCGATTCTGGCGGCAGCGGGGCAGGGTGTGGTCGAGGCGCAGTTGCTGCTGGGGCAGATCCTGCTGGATGGGCGTGGCATCCAGCAGGATGCCACGGTTGCCCGGC  >  NC_002947/1000928‑1001216
                                                                                                                                               |                                                                                                                                                 
cAGCAACTGACTGCCGATGTGCCGGACCATCCTTCGCTTTTGCAGCTGACGGGCACCTACCATAATTTGCTGCGCCGCTGGGCTGAGGTCTGAGCCGTGTCCTACCTGCTGCGACGTGAGGAAGTGGTGGATGTGGCCGGCTTGCAGGc                                                                                                                                              <  1:180541/149‑1 (MQ=255)
cAGCAACTGACTGCCGATGTGCCGGACAATCCTTCGCTTTTGCAGCTGACGGGCACCTACCATAATTTGCTGCGCCGCAGGGCTGAGGTCTGAGCAGTCTCCTACCTGCTGCGACGTGAGGAAGTGGTGGATGTGGCCGGCTTGCAGGc                                                                                                                                              >  5:357783/1‑149 (MQ=255)
   cAACTGACTGCCGATGTGCCGGACCATCCTTCGCTTTTGCAGCTGACGGGCACCTACCATAATTTGCTGCGCCGCTGGGCTGAGGTCTGAGCCGTGTCCTACCTGCTGCGACGTGAGGAAGTGGTGGATGTGGCCTGCAAGCCGGCCac                                                                                                                                           <  6:291696/149‑2 (MQ=255)
   cAACTGACTGCCGATGTGCCGGACCATCCTTCGCTTTTGCAGCTGACGGGCACCTACCATAATTTGCTGCGCCGCTGGGCTGAGGTCTGAGCCGTGTCCTACCTGATGCGACGTGAGGAAGTGGTGGATGTGGCCGGCTTGCAAGCCa                                                                                                                                            <  6:134483/148‑1 (MQ=255)
   cAACTGACTGCCGATGTGCAGGACCATCCTTCGCTTTTGCAGCTGACGGGCACCTACCATAATTTGCTGCGCCGCTGGGCTGAGGTCTGAGCCGTGTCCTACCTGCTGCGACGTGAGGAAGTGGTGGATGTGGCCGGCTTGCAGGCCAt                                                                                                                                           >  5:103056/1‑149 (MQ=255)
      ctgactgCCGATGTGCCGGACCATCCTTCGCTTTTGCAGCTGACGGGCACCTACCATAATTTGCTGCGCCGCTGGGCTGAGGTCTGAGCCGTGTCCTACCTGCTGCGACGTGAGGAAGTCGTGGATGTGGCCGGCTTGCAGGCCATGCt                                                                                                                                        >  1:330487/1‑149 (MQ=255)
        gactgCCGATGCGCCGGACCATCCTTCGCTTTTGCAGCTGACGGGCACCTACCATAATTTGCTGCGCCGCTGGGCTGAGGTCTGAGCCGTGTCCTACCTGCTGCGACGTGAGGAAGTGGTGGATGTGGCCGGCTTGCAGGCCATGCTc                                                                                                                                       >  4:165621/1‑148 (MQ=255)
           tgCCGATGTGCCGGACCATCCTTCGCTTTTGCAGCTGACGGGCACCTACCATAATTTGCTGCGCCGCTGGGCTGAGGTCTGAGCCGTGTCCTACCTGCTGCGACGTGAGGAAGTGGTGGATGTGGCCGGCTTGCAGGCCATGCTCGAg                                                                                                                                    >  5:247353/1‑148 (MQ=255)
            gCCGATGTGCCGGACCATCCTTCGCTTTTGCAGCTGACGGGCACCTACCATAATTTGCTGCGCCGCTGGGCTGAGGTCTGAGCCGTGTCCTACCTGCTGCGACGTGAGGAAGTGGTGGATGTGGCCGGCTTGCAGGCCATGCTCGAgg                                                                                                                                   >  4:319316/1‑148 (MQ=255)
              cGATGTGCCGGACCATCCTTCGCTTTTGCAGCTGACGGGCACCTACCATAATTTGCTGCGCCGCTGGGCTGAGGTCTGAGCCGTGTCCTACCTGCTGCGACGTGAGGAAGTGGTGGATGTGGCCGGCTTACAGGCCATGCTCGAGGaaa                                                                                                                                <  3:327476/149‑1 (MQ=255)
                   tgCCGGACCATCCTTCGCTTTTGCAGCTGACGGGCACCTACCATAATTTGCTGCGCCGCTGGGCTGAGGTCTGAGCCGTGTCCTACCTGCTGCGACGTGAGGAAGTGGTGGATGTGGCCGGCTTGCAGGCCATGCTCGAGGAAACTccc                                                                                                                           <  6:103056/149‑1 (MQ=255)
                        gACCATCCTTCGCTTTTGCAGCTGACGGGCACCTACCATAATTTGCTGCGCCGCTGGGCTGAGGTCTGAGCCGTGTCCTACCTGCTGCGACGTGAGGAAGTGGTGGATGTGGCCGGCTTGCAGGCCGTGCTCGAGGAAACTCCCGGc                                                                                                                        >  3:314467/1‑147 (MQ=255)
                               tttCGCTTTTGCAGCTGACGGGCACCTACCATAATTTGCTGCGCCGCTGGGCTGAGGGCTGAGCCGTGTCCTACCTGCTGCGACGTGAGGAAGTGGTGGATGTGGCCGGCTTGCAGGCCATGCTCGAGGAAACTCCCg                                                                                                                          <  4:255502/137‑1 (MQ=255)
                                ttCGCTTTTGCAGCTGACGGGCACCTACCATAATTTGCTGCGCCGCTGGGCTGAGGTCTGAGCCGTGTCCTACCTGCTGCGACGTGAGGAAGTGGTGGATGTGGCCGGCTTGCAGGCCATGCTCGAGGAAACTCCCGGCAAGgccgcc                                                                                                               >  7:174637/1‑148 (MQ=255)
                                                    gCACCTACCATAATTTGCTGCGCCGCTGGGCTGAGGTCTGAGCCGTGTCCTACCTGCTGCGACGTGAGGAAGTGGTGGATGTGGCCGGCTTGCAGGCCATGCTCGAGGAAACTCCCGGCAAGGCCGCCCAgg                                                                                                           <  5:86358/132‑1 (MQ=255)
                                                    gCACCTACCATAATTTGCTGCGCCGCTGGGCTGAGGTCTGAGCCGTGTCCTACCTGCTGCGACGTGAGGAAGTGGTGGATGTGGCCGGCTTGCAGGCCATGCTCGAGGAAACTCCCGGCAAGGCCGCCCAgg                                                                                                           >  6:86358/1‑132 (MQ=255)
                                                        cTACCATAATTTGCTGCGCCGCTGGGCTGAGGTCTGAGCCGTGTCCTACCTGCTGCGACGTGAGGAAGTGGTGGATGTGGCCGGCTTGCAGGCCATGCTCGAGGAAACTCCCGGCAAGGCCGCCCAGGCGATTCTGGCGGCAGCgggg                                                                                       >  3:22593/1‑148 (MQ=255)
                                                          aCCATAATTTGCTGCGCCGCTGGGCTGAGGTCTGAGCCGTGTCCTACCTGCTGCGACGTGAGGAAGTGGTGGATGTGGCCGGCTTGCAGGCCATGCTCGa                                                                                                                                     >  8:224896/1‑100 (MQ=255)
                                                          aCCATAATTTGCTGCGCCGCTGGGCTGAGGTCTGAGCCGTGTCCTACCTGCTGCGACGTGAGGAAGTGGTGGATGTGGCCGGCTTGCAGGCCATGCTCGa                                                                                                                                     <  7:224896/100‑1 (MQ=255)
                                                           ccATAATTTGCTGCGCCGCTGGGCTGAGGTCTGAGCCGTGTCCTACCTGCTGCGACGTGAGGAAGTGGTGGATGTGGCCGGCTTGCAGGCCATGCTCGAGGAAACTCCCGGCAAGGCCGCCCAGGCGATTCTGGCGGCAGCGGGGCAgg                                                                                   <  4:22593/149‑1 (MQ=255)
                                                            cATAATTTGCTGCGCCGCTGGGCTGAGGTCTGAGCCGTGTCCTACCTGCTGCGACGTGAGGAAGTGGTGGATGTGGCCGGCTTGCAGGCCATGCTCGAGGAAACTCCCGGCAAGGCCGCCCAGGCGATTCTGGCGGCAGCGGGGCAggg                                                                                  <  8:174637/149‑1 (MQ=255)
                                                            cATAATTTGCTGCGCCGCTGGGCTGAGGTCTGAGCCGTGTCCTACATGCTGCGACGTGAGGAAGTGGTGGATGTGGCCGGCTTGCAGGCCATGCTCGAGGAAACTCCCGGCAAGGCCGCCCAGGCGATTCTGGCGGCAGCGGGGCAggg                                                                                  >  4:88426/1‑149 (MQ=255)
                                                             aTAATTTGCTGCGCCGCTGGGCTGAGGTCTGAGCCGTGTCCTACCTGCTGCGACGTGAGGAAGTGGTGGATGTGGCCGGCTTGCAGGCCATGCTCGAGGAAACTCCCGGCAAGGCCGCCCAGGCGATTCTGGCGGCAGCGGGGCAGGgt                                                                                 <  3:319316/149‑1 (MQ=255)
                                                                 tttGCTGCGCCGCTGGGCTGAGGTCTGAGCCGTGTCCTACCTGCTGCGACGTGAGGAAGTGGTGGATGTGGCCGGCTTGCAGGCCATGCTCGAGGAAACTCCCGGCAAGGCCGCCCAGGCGATTCTGGCGGCAGCgggg                                                                                       >  7:31137/1‑139 (MQ=255)
                                                                 tttGCTGCGCCGCTGGGCTGAGGTCTGAGCCGTGTCCTACCTGCTGCGACGTGAGGAAGTGGTGGATGTGGCCGGCTTGCAGGCCATGCTCGAGGAAACTCCCGGCAAGGCCGCCCAGGCGATTCTGGCGGCAGCgggg                                                                                       <  8:31137/139‑1 (MQ=255)
                                                                     ctgcGCCGCTGGGCTGAGGTCTGAGCCGTGTCCTACCTGCTGCGACGTGAGGAAGTGGTGGATGTGGCCGGCTTGCAGGCCATGCTCGAGGAAACTCCCGGCAAGGCCGCCCAGGCGATTCTGGCGGCAGCGGGGCAGGGTGTGGTCGa                                                                         <  6:247353/149‑1 (MQ=255)
                                                                      tgcGCCGCTGGGCTGAGGTCTGAGCCGTGTCCTACCTGCTGCGACGTGAGGAAGTGGTGGATGTGGCCGGCTTGCAGGCCATGCTCGAGGAAACTCCCGGCAAGGCCGCCCAGGCGATTCTGGCGGCAGCGGGGCAGGGTGTGGTCGAg                                                                        >  8:238276/1‑149 (MQ=255)
                                                                               gggCTGAGGTCTGAGCCGTGTCCTACCTGCTGCGACGTGAGGAAGTGGTGGATGTGGCCGGCTTGCAGGCCATGCTCGAGGAAACTCCCGGCAAGGCCGCCCAGGCGATTCTGGCGGCAGCGGGGCAGGGTGTGGTCGAGGCGCAGTtg                                                               >  4:65010/1‑149 (MQ=255)
                                                                                  cTGAGGTCTGAGCCGTGTCCTACCTGCTGCGAGGTGAGGAAGTGGTGGATGTGGCCGGCTTGCAGGCCATGCTCGAGGTAACTCCCGGCAAGGCCGCCCAGGCGATTCTGGCGGCAGCGGGGCAGGGTGTGGTCGAGGCGCAGTtgctg                                                            <  6:357783/149‑1 (MQ=255)
                                                                                      gggcTGAGCCGGGTCCTCCCTGCTGCGACGTGAGGAAGTGGTGGATGTGGCCGGCTTGCAGGCCATGCTCGAGGAAACTCCCGGCAAGGCCGCCCAGGCGATTCTGGCGGCAGCGGGGCAGGGTGTGGTCGAGGCGCAGTTGCTGCTgg                                                        <  5:326664/146‑1 (MQ=255)
                                                                                      ggTCTGAGCCGTGTCCTACCTGCTGCGACGTGAGGAAGTGGTGGATGTGGCCGGCTTGCAGGCCATGCTCGAGGAAACTCCCGGCAAGGCCGCCCAGGCGATTCTGGCGGCAGCGGGGCAGGGTGTGGTCGAGGCGCAGTTGCTGCTgg                                                        <  3:88426/149‑1 (MQ=255)
                                                                                       gTCTGAGCCGTGTCCTACCTGCTGCGACGTGAGGAAGTGGTGGATGTGGCCGGCTTGCAGGCCATGCTCGAGGAAACTCCCGGCAAGGCCGCCCAGGCGATTCTGGCGGCAGCGGGGCAGGGTGTGGTCGAGGCGCAGTTGCTGCTggg                                                       <  3:165621/149‑1 (MQ=255)
                                                                                        tCTGAGCCGTGTCCTACCTGCTGCGACGTGAGGAAGTGGTGGATGTGGCCGGCTTGCAGGCCATGCTCGAGGAAACTCCCGGCAAGGCCGCCCAGGCGATTCTggcg                                                                                                <  6:352902/107‑1 (MQ=255)
                                                                                        tCTGAGCCGTGTCCTACCTGCTGCGACGTGAGGAAGTGGTGGATGTGGCCGGCTTGCAGGCCATGCTCGAGGAAACTCCCGGCAAGGCCGCCCAGGCGATTCTggcg                                                                                                >  5:352902/1‑107 (MQ=255)
                                                                                            aGCCGTGTCCTACCTGCTGCGACGTGAGGAAGTGGTGGATGTGGCCGGCTTGCAGGCCGTGCTCGAGGAAACTCCCGGCAAGGCCGCCCAGGCGATTCTGGCGGCAGCGGGGCAGGGTGTGGTCGAGGCGCAGTTGCTGCTGGGGc                                                     <  4:314467/146‑1 (MQ=255)
                                                                                            aGCCGTGTCCTACCTGCTGCGACGTGAGGAAGTGGTGGATGTGGCCGGCTTGCAGGCCATGCTCGAGGAAACTCCCGGCAAGGCCGCCCAGGCGATTCTGGCGGCAGCGGGGCAgg                                                                                   >  5:251841/1‑116 (MQ=255)
                                                                                            aGCCGTGTCCTACCTGCTGCGACGTGAGGAAGTGGTGGATGTGGCCGGCTTGCAGGCCATGCTCGAGGAAACTCCCGGCAAGGCCGCCCAGGCGATTCTGGCGGCAGCGGGGCAgg                                                                                   <  6:251841/116‑1 (MQ=255)
                                                                                             gCCGTGTCCTACCTGCTGCGACGTGAGGAAGTGGTGGATGTGGCCGGCTTGCAGGCCATGCTCGAGGAAACTCCCGGCAAGGCCGCCCAGGCGATTCTGGCGGCAGCGGGGCAGGGTGTGGTCGAGGCGCAGTTGCTGCTgggg                                                      >  6:329361/1‑144 (MQ=255)
                                                                                                gtgtCCTACCTGCTGCGACGTGAGGAAGTGGTGGATGTGGCCGGCTTGCAGGCCATGCTCGAGGAAACTCCCGGCAAGGCCGCCCAGGCGATTCTGGCGGCAGCGGGGCAGGGTGTGGTCGAGGCGCAGTTGCTGCTGGGGCAGATCct                                              >  8:54158/1‑149 (MQ=255)
                                                                                                      tACCTGCTGCGACGTGAGGAAGTGGTGGATGTGGCCGGCTTGCAGGCCATGCTCGAGGAAACTCCCGGCAAGGCCGCCCAGGCGATTCTGGCGGCAGCGGGGCAGGGTGTGGTCGAGGCGCAGTTGCTGCTgggg                                                      <  6:339825/135‑1 (MQ=255)
                                                                                                      tACCTGCTGCGACGTGAGGAAGTGGTGGATGTGGCCGGCTTGCAGGCCATGCTCGAGGAAACTCCCGGCAAGGCCGCCCAGGCGATTCTGGCGGCAGCGGGGCAGGGTGTGGTCGAGGCGCAGTTGCTGCTgggg                                                      >  5:339825/1‑135 (MQ=255)
                                                                                                      tACCTGCTGCGACGTGAGGAAGTGGTGGATGTGGCCGGCTTGCAGGCCATGCTCGAGGAAACTCCCGGCAAGGCCGCCCAGGCGATTCTGGCGGCAGCGGGGCAGGGTGTGGTCGAGGCGCAGTTGCTGCTGGGGCAGATCCTGCTGGa                                        >  6:123040/1‑149 (MQ=255)
                                                                                                      tACCTGCTGCGACGTGAGGAAGTGGTGGATGTGGCCGGCTTACAGGCCATGCTCGAGGAAACTCCCGGCAAGGCCGCCCAGGCGATTCTGGCGGCAGCg                                                                                          <  8:220371/99‑1 (MQ=255)
                                                                                                      tACCTGCTGCGACGTGAGGAAGTGGTGGATGTGGCCGGCTTACAGGCCATGCTCGAGGAAACTCCCGGCAAGGCCGCCCAGGCGATTCTGGCGGCAGCg                                                                                          <  8:216100/99‑1 (MQ=255)
                                                                                                      tACCTGCTGCGACGTGAGGAAGTGGTGGATGTGGCCGGCTTACAGGCCATGCTCGAGGAAACTCCCGGCAAGGCCGCCCAGGCGATTCTGGCGGCAGCg                                                                                          >  7:220371/1‑99 (MQ=255)
                                                                                                      tACCTGCTGCGACGTGAGGAAGTGGTGGATGTGGCCGGCTTACAGGCCATGCTCGAGGAAACTCCCGGCAAGGCCGCCCAGGCGATTCTGGCGGCAGCg                                                                                          >  7:216100/1‑99 (MQ=255)
                                                                                                              gcGACGTGAGGAAGTGGTGGATGTGGCCGGCTTGCAGGCCATGCTCGAGGAAACTCCCGGCAAGGCCGCCCAGGCGATTCTGGCGGCAGCGGGGCAGGGtgtg                                                                              <  4:334290/103‑1 (MQ=255)
                                                                                                               cGACGTGAGGAAGTGGTGGATGTGGCCGGCTTGCAGGCCATGCTCGAGGAAACTCCCGGCAAGGCCGCCCAGGCGATTCTGGCGGCAGCGGGGCAGGGTGTGGTCGAGGCGCAGTTGCTGCTGGGGCAGATCCTGCTGGATGGGCGTgg                               >  5:90514/1‑149 (MQ=255)
                                                                                                               cGACGTGAGGAAGTGGTGGATGTGGCCGGCTTGCAGGCCATGCTCGAGGAAACTCCCGGCAAGGCCGCCCAGGCGATTCTGGCGGCAGCGGGGCAGGGTGTGGTCGAGGCGCAGTTGCTGCTGGGGCAGATCCTGCTGGATGGGCGTgg                               <  7:238276/149‑1 (MQ=255)
                                                                                                               cGACGTGAGGAAGTGGTGGATGTGGCCGGCGTGCAGGCCATGCTCGAGGAAACTCCCGGCAAGGCCGCCCAGGCGATTCTGGCGGCAGCGGGGCAGGGTGTGGTCGAGGCGCAGTTGCTGCTGGGGCAGATCCTGCTGGATGGGCGTg                                <  8:150853/148‑1 (MQ=255)
                                                                                                                    tGAGGAAGTGGTGGATGTGGCCGGCTTGCAGGCCATGCTCGAGGAAACTCCCGGCAAGGCCGCCCAGGCGATTCTGGCGGCAGCGGGGCAGGGTGTGGTCGAGGCGCAg                                                                  <  2:119414/109‑1 (MQ=255)
                                                                                                                      aGGAAGTGGTGGATGTGGCCGGCTTGCAGGCCATGCTCGAGGAAACTCCCGGCAAGGCCGCCCAGGCGATTCTGGCGGTAGCGGGGCAGGGTGTGGTCGAGGCGCAGTTGCTGCTGGGGCAGATCCTGCTGGATGGGCGTGGCATCcag                        >  3:180199/1‑149 (MQ=255)
                                                                                                                      aGGAAGTGGTGGATGTGGCCGGCTTGCAGGCCATGCTCGAGGAAACTCCCGGCAAGGCCGCCCAGGCGATTCTGGCGGCAGCGGGGCAGGGTGTGGTCGAGGCGCAGTTGATGCTGGGGCAGATCctgct                                           >  7:309479/1‑130 (MQ=255)
                                                                                                                        gAAGTGGTGGATGTGGCCGGCTTGCAGGCCATGCTCGAGGAAACTCCCGGCAAGGCCGCCCAGGCGACTCTGGCGGCAGCGGGGCAGGGTGTGGTCGAGGCGCAGTTGCTGCTGGGGCAGATCctg                                             >  6:354507/1‑126 (MQ=255)
                                                                                                                        gAAGTGGTGGATGTGGCCGGCTTGCAGGCCATGCTCGAGGAAACTCCCGGCAAGGCCACCCAGGCGATTCTGGCGGCAGCGGGGCAGGGTGTGGTCGAGGCGCAGTTGCTGCTGGGGCAGATCCTGCTGGATGGGCGTGGCATCcag                        >  1:269811/1‑147 (MQ=255)
                                                                                                                         aaGTGGTGGATGTGGCCGGCTTGCAGGCCATGCTCGAGGAAACTCCCGGCAAGGCCGCCCAGGCGATTCTGGCGGCAGCGGGGCAGGGTGTGGTCGAGGCGCAGTTGCTGCTGGGGCAGATCCTGCTGGATGGGCGTGGCATCcagcag                     >  6:98181/1‑149 (MQ=255)
                                                                                                                            tggtggATGTGGCCGGCTTGCAGGCCATGCTCGAGGAAACTCCCGGCAAGGCCGCCCAGGCGATTCTGGCGGCAGCGGGGCAGGGTGTGGTCGAGGCGCAGTTGCTGCTGGGGCAGATCCTGCTGGATGGGCGTGGCATCCAGCAGGAt                  <  2:269811/149‑1 (MQ=255)
                                                                                                                              gtggATGTGGCCGGCTTGCAGGCCATGCTCGAGGAAACTCCCGGCAAGGCCGCCCAGGCGATTCTGGCGGCAGCGGGGCAGGGTGTGGTCGAGGCGCAGTTGCTGCTGGGGCAGATCCTGCTGGATGGGCGTg                                <  4:69338/133‑1 (MQ=255)
                                                                                                                              gtggATGTGGCCGGCTTGCAGGCCATGCTCGAGGAAACTCCCGGCAAGGCCGCCCAGGCGATTCTGGCGGCAGCGGGGCAGGGTGTGGTCGAGGCGCAGTTGCTGCTGGGGCAGATCCTGCTGGATGGGCGTGGCATCCAGCAGGATGc                >  4:276183/1‑149 (MQ=255)
                                                                                                                                ggATGTGGCCGGCTTGCAGGCCATGCTCGAGGAAACTCCCGGCAAGGCCGCCCAGGCGATTCTGGCGGCAGCGGGGCAGGGTGTGGTCGAGGCGCAGTTGCTGCTGGGGCAGATCCTGCTGGATGGGCGTGGCATCCAGCAGGATGCCa              >  5:153177/1‑149 (MQ=255)
                                                                                                                                 gATGTGGCCGGCTTGCAGGCCATGCTCGAGGAAACTCCCGGCAAGGCCGCCCAGGCGATTCTGGCGGCAGCGGGGCAGGGTGTGGTCGAGGCGCAGTTGCTGCTGGGGCAGATCCTGCTGGATGGGCGTGGCATCCAGCAGGATGCCAc             >  4:25270/1‑149 (MQ=255)
                                                                                                                                 gATGTGGCCGGCTTGCAGGCCATGCTCGAGGAAACTCCCGGCAAGGCCGCCCAGGCGATTCTGGCGGCAGCGGGGCAGGGTGTGGTCGAGGCGCAGTTGCTGCTGGGGCAGATCCTGCTGGATGGGCGTGGCATCCAGCAGGATGCCAc             >  2:75191/1‑149 (MQ=255)
                                                                                                                                 gATGTGGCCGGCTTGCAGGCCATGCTCGAGGAAACTCCCGGCAAGGCCGCCCAGGCGATTCTGGCGGCAGCGGGGCAGGGTGTGGTCGAGGCGCAGTGGCTGCTGGGGCAGATCCTGCTGGATGGGCGTGGCATCCAGCAGGATGCCAc             <  3:25270/149‑1 (MQ=255)
                                                                                                                                    gtgGCCGGCTTACAGGCCATGCTCGAGGAAACTCCCGACAAGGCCGCCCAGGCGATTCTGGCGGCAGCGGGGCAGGGTGTGGTCGAGGCGCAGTTGCTGCTGGGGCAGATCCTGCTGGATGGGCGTGGCATCCAGCAGGATGCCACGGt          >  8:53002/1‑149 (MQ=255)
                                                                                                                                     tgGCCGGCTTGCAGGCCATGCTCGAGGAAACTCCCGGCAAGGCCGCCCAGGCGATTCTGGCGGCAGCGGGGCAGGGTGTGGTCGAGGCGCAGTTGCTGCTGGGGCAGATCCTGCTGGATGGGCGTGGCATCCAGCAGGATGCCACGGtt         <  7:54158/149‑1 (MQ=255)
                                                                                                                                       gCCGGCTTGCAGGCCATGCTCGAGGAAACTCCCGGCAAGGCCGCCCAGGCGATTCTGGCGGCAGCGGGGCAGGGTGTGGTCGAGGCGCAGTTGCTGCTGGGGCAGATCCTGCTGGATGGGCGTGGCATCCAGCAGGATGCCACGGTTGc       >  6:289647/1‑149 (MQ=255)
                                                                                                                                       gCCGGCTTGCAGGCCATGCTCGAGGAAACTCCCGGCAAGGCCGCCCAGGCGATTCTGGCGGCAGCGGGGCAGGGTGTGGTCGAGGCGCAGTTGCTGCTGGGGCAGATCCTGCTGGATGGGCGTGGCATCCAGCAGGATGCCACGGTTGc       >  8:188478/1‑149 (MQ=255)
                                                                                                                                         cGGCTTGCAGGCCATGCTCGAGGAAACTCCCGGCAAGGCCGCCCAGGCGATTCTGGCGGCAGCGGGGCAGGGTGTGGTCGAGGCGCAGTTGCTGCTGGGGCAGATCCTGCTGGATGGGCGTGGCATCCAGCAGGATGCCACGGTTGccc     >  8:313287/1‑149 (MQ=255)
                                                                                                                                            cTTGCAGGCCATGCTCGAGGAAACTCCCGGCAAGGCCGCCCAGGCGATTCTGGCGGCAGCGGGGCAGGGTGTGGTCGAGGCGCAGTTGCTGCTGGGGCAGATCCTGCTGGATGGGCGTGGCATCCAGCAGGATGCCACGGTTGCCCGGc  <  3:65010/149‑1 (MQ=255)
                                                                                                                                               gCAGGCCATGCTCGAGGAAACTCCCGGCAAGGCCGCCCAGGCGATTCTGGCGGCAGCGGGGCAGGGTGTGGTCGAGGCGCAGTTGCTGCTGGGGCAGATCCTGCTGGATGGGCGTGGCATCCAGCAGGATGCCACGGTTGCCCg    <  2:178588/144‑1 (MQ=255)
                                                                                                                                               gCAGGCCATGCTCGAGGAAACTCCCGGCAAGGCCGCCCAGGCGATTCTGGCGGCAGCGGGGCAGGGTGTGGTCGAGGCGCAGTTGCTGCTGGGGCAGATCCTGCTGGATGGGCGTGGCATCCAGCAGGATGCCACGGTTGCCCg    >  1:178588/1‑144 (MQ=255)
                                                                                                                                               |                                                                                                                                                 
CAGCAACTGACTGCCGATGTGCCGGACCATCCTTCGCTTTTGCAGCTGACGGGCACCTACCATAATTTGCTGCGCCGCTGGGCTGAGGTCTGAGCCGTGTCCTACCTGCTGCGACGTGAGGAAGTGGTGGATGTGGCCGGCTTGCAGGCCATGCTCGAGGAAACTCCCGGCAAGGCCGCCCAGGCGATTCTGGCGGCAGCGGGGCAGGGTGTGGTCGAGGCGCAGTTGCTGCTGGGGCAGATCCTGCTGGATGGGCGTGGCATCCAGCAGGATGCCACGGTTGCCCGGC  >  NC_002947/1000928‑1001216

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑