Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 1,013,235 A→T 12.6% intergenic (+63/+68) prfC → / ← potF‑I peptide chain release factor 3/putrescine‑binding protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029471,013,2350AT12.6% 72.7 / 4.2 32intergenic (+63/+68)prfC/potF‑Ipeptide chain release factor 3/putrescine‑binding protein
Reads supporting (aligned to +/- strand):  ref base A (16/11);  new base T (2/2);  total (18/14)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.87e-01

ACCTGGCGCCGACCCGGGTCAACCTGGCGCTGATGGAAGAGCGCTGGCCGGACATCAAGTTCCGCGCTACCCGCGAGCACCATTAAGGCCTGAAATCGGGGCTGCTTTGCAGCCCTTCGCGGGCACGCCCGCTCCCACAGGAATCA‑CCAGACCTGGAGGTCATGTGATACCTGTGGGAGCGGGCGTGCCCGCGAAGGGCCGCTAGGC‑GGCCCCATTTCAATTTCCGGCTTTGATACTGGTCCAGATTCGCGTACGAATACGGTCGATCTTCGCCGGCATCGCCTCCAGCGCATACA  >  NC_002947/1013087‑1013382
                                                                                                                                                     |                                                                                                                                                    
acctGGCGCCGACCCGGGTCAACCTGGCGCTGATGGAAGAGCGCTGGCCGGACATCAAGTTCCGCGCTACCCGCGAGCACCATTAAGGCCTGAAATCGGGGCTGCTTTGCAGCCCTTCGCGGGCACGCCCGCTCCCACAGGAATCA‑CCa                                                                                                                                                      >  8:243555/1‑149 (MQ=255)
           aCCCGGGTCAACCTGGCGCTGATGGAAGAGCGCTGGCCGGACATCAAGTTCCGCGCTACCCGCGAGCACCATTAAGGCCTGAAATCGGGGCTGCTTTGCAGCCCTTCGCGGGCACGCCCGCTCCCACAGGTATCA‑CATGACCTCCAGGt                                                                                                                                           >  4:233471/1‑149 (MQ=38)
              cGGGTCAACCTGGCGCTGATGGAAGAGCGCTGGCCGGACATCAAGTTCCGCGCTACCCGCGAGCACCATTAAGGCCTGAAATCGGGGCTGCTTTGCAGCCCTTCGCGGGCACGCCCGCTCCCACAGGAATCA‑CCAGACCTGGAGGTCAt                                                                                                                                        <  7:243555/149‑1 (MQ=255)
                    aaCCTGGCGCTGATGGAAGAGCGCTGGCCGGACATCAAGTTCCGCGCTACCCGCGAGCACCATTAAGGCCTGAAATCGGGGCTGCTTTGCAGCCCTTCGCGGGCACGCCCGCTCCCACAGGAATCA‑CCAGACCTGGAGGTCATGTGATa                                                                                                                                  >  1:316936/1‑149 (MQ=255)
                          gcgcTGATGGAAGAGCGCTGGCCGGACATCAAGTTCCGCGCTACCCGCGAGCACCATTAAGGCCTGAAATCGGGGCTGCTTTGCAGCCCTTCGCGGGCACGCCCGCTCCCACAGGAATCA‑CCAGACCTGGAGGTCATGTGATACCtg                                                                                                                              <  2:316936/147‑1 (MQ=255)
                           cgcTGATGGAAGAGCGCTGGCCGGACATCAAGTTCCGCGCTACCCGCGAGCACCATTAAGGCCTGAAATCGGGGCTGCTTTGCAGCCCTTCGCGGGCACGCCCGCTCCCACAGGTATCA‑CATGACCTCCAGGTCTGGTGATTCCTGTgg                                                                                                                           <  3:233471/149‑1 (MQ=25)
                               gATGGAAGAGCGCTGGCCGGACATCAAGTTCCGCGCTACCCGCGAGCACCATTAAGGCCTGAAATCGGGGCTGCTTTGCAGCCCTTTGCGGGCACGCCCGCTCCCACAGGAATCA‑CCAGACCTGGAGGTCAtgt                                                                                                                                      <  5:188781/134‑1 (MQ=255)
                               gATGGAAGAGCGCTGGCCGGACATCAAGTTCCGCGCTACCCGCGAGCACCATTAAGGCCTGAAATCGGGGCTGCTTTGCAGCCCTTTGCGGGCACGCCCGCTCCCACAGGAATCA‑CCAGACCTGGAGGTCAtgt                                                                                                                                      >  6:188781/1‑134 (MQ=255)
                                  ggAAGAGCGCTGGCCGGACATCAAGTTCCGCGCTACCCGCGAGCACCATTAAGGCCTGAAATCGGGGCTGCTTTGCAGCCCTTCGCGGGCCCGCCCCCTCCCACACGAAACA‑CCACACCAGGTGGTCATGTGTTAACGGTTGGGGCggg                                                                                                                    >  5:31397/1‑149 (MQ=37)
                                     cccgatcTGGCCGGACATCAAGTTCCGCGCTACCCGCGAGCACCATTAAGGCCTGAAATCGGGGCTGCTTTGCAGCCCTTCGCGGGCACGCCCGCTCCCACAGGAATCACCCAGACCTGGAGGTCATGTGATACCTGTGGGAGCgg                                                                                                                     <  1:53092/140‑1 (MQ=255)
                                              gCCGGACATCAAGTTCCGCGCTACCCGCGAGCACCATTAAGGCCTGAAATCGGGGCTGCTTTGCAGCCCTTCGCGGGCACGCCCGCTCCCACAGGAATCA‑CCAGACCTGGAGGTCATGTGATACCTGTGGGAGCgg                                                                                                                     >  2:53092/1‑136 (MQ=255)
                                               ccGGACATCAAGTTCCGCGCTACCCGCGAGCACCATTAAGGCCTGAAATCGGGGCTGCTTTGCAGCCCTTCGCGGGCACGCCCGCTCCCACAGGAATCA‑CCAGACCTGGAGGTCAtgt                                                                                                                                      >  1:305912/1‑118 (MQ=255)
                                               ccGGACATCAAGTTCCGCGCTACCCGCGAGCACCATTAAGGCCTGAAATCGGGGCTGCTTTGCAGCCCTTCGCGGGCACGCCCGCTCCCACAGGAATCA‑CCAGACCTGGAGGTCAtgt                                                                                                                                      <  2:305912/118‑1 (MQ=255)
                                                                      ccGCGAGCACCATTAAGGCCTGAAATCGGGGCTGCTTTGCAGCCCTTCGCGGGCACGCCCGCTCCCACAGGAATCA‑CCAGACCTGGAGGTCATGTTATACCTGTGGGAGCGGGCGTGCCCGCGAAg                                                                                                       <  4:256615/126‑1 (MQ=255)
                                                                      ccGCGAGCACCATTAAGGCCTGAAATCGGGGCTGCTTTGCAGCCCTTCGCGGGCACGCCCGCTCCCACAGGAATCA‑CCAGACCTGGAGGTCATGTGATACCTGTGGGAGCGGGCGTGCCCGCGAAg                                                                                                       >  3:256615/1‑126 (MQ=255)
                                                                                cATTAAGGCCTGGAATCGGGGCTGCTTTGCAGCCCTTCGCGGGCCCGCCCCCACCCCCCAGAAAAA‑CCACAACAGGAGGGCATGTGATAACAGTGGGAGCGGGGGTGCCCGCGAAGGGGCCCGAGGCGGGCCCCATTTAAAtttaggcg                                                                      >  8:303773/1‑144 (MQ=255)
                                                                                           gAAATCGGGGCTGCTTTGCAGCCCTTCGCGGGCACGCCCGCTCCCACAGGAATCA‑CCAGACCTGGAGGTCATGTGAt                                                                                                                                   >  8:221939/1‑77 (MQ=255)
                                                                                           gAAATCGCGGCTGCTTTGCATCCCTTCGCGGGGCCGCCCGCTCCCACACGAATCC‑CCAGACCTGGAGGTCATGTGAt                                                                                                                                   <  7:221939/77‑1 (MQ=255)
                                                                                                     ctgctTTGCAGCCCTTCGCGGCCACCCCCGCTCCCACAGGAATCA‑CCACCCCTGGAGGTAATGTTATACCTGTGGGAGCGGGCGTGCCCGCGAAGGGCCGCTAGGC‑GGCCCCATTTCAATTTCCGGCTTTGATACTGGTCCAGATTcgc                                                <  7:303773/149‑1 (MQ=38)
                                                                                                          ttGCCGCCCTTCGCGGGCACGCCCGCTCCCACAGGAATCA‑CCAGACCTGGAGGTCATGTGATACCTGTGGGAGCGGGCGTGCCCGCGAAGGGCCGCTAGGC‑GGCCCCATTTCAATTTcc                                                                         <  7:327006/119‑1 (MQ=255)
                                                                                                                cccTGCGGGGCCCCGCCCGCCCCCACCGCATTCC‑CCCGCCCTGTTGGTCTTGTTATCCGGGTGGGAGGGGGGGTGCCCGCGAAGGGCCGCTAGGC‑GGCCCCATTTCAATTTCCGGCTTTGATACTGGTCCAGATTCGCGTACGAATACg                                     <  6:31397/149‑1 (MQ=255)
                                                                                                                              gCCCGCTCCCACAGGAATCA‑CCAGACCTGGAGGTCATGTGATACCTGTGGGAGCGGGCGTGCCCGCGAAGGGCCGCTAGGC‑GGCCCCATTTCAATTTCCGGCTTTGATACTGGTCCAGATTCGCGTATGAATACGGTCGATCTTCGCCg                       >  1:141677/1‑149 (MQ=255)
                                                                                                                                       cacaGGAATCA‑CCAGACCTGGAGGTCATGTGATACCTGTGGGAGCGGGCGTGCCCGCGAAGGGCCGCTAGGC‑GGCCCCATTTCAATTTCCGGCTTTGATACTGGTCCAGATTCGCGTACGAATACGGTCGATCTTc                           >  8:201182/1‑136 (MQ=255)
                                                                                                                                       cacaGGAATCA‑CCAGACCTGGAGGTCATGTGATACCTGTGGGAGCGGGCGTGCCCGCGAAGGGCCGCTAGGC‑GGCCCCATTTCAATTTCCGGCTTTGATACTGGTCCAGATTCGCGTACGAATACGGTCGATCTTc                           <  7:201182/136‑1 (MQ=255)
                                                                                                                                       cacaGGAATCA‑CCAGACCTGGAGGTCATGTGATACCTGTGGGAGCGGGCGTGCCCGCGAAGGGCCGCTAGGC‑GGCCCCATTTCAATTTCCGGCTTTGATACTGGTCCAGATTCGCGTACGAATACGGTCGATCTTCGCCGGCATCGCCt              >  8:96954/1‑149 (MQ=255)
                                                                                                                                       cacaGGAATCA‑CCAGACCTGGAGGTCATGTGATACCTGTGGGAGCGGGCGTGCCCGCGAAGGGCCGCTAGGC‑GGCCCCATTTCAATTTCCGGCTTTGATACTGGTCCAGATTCGCGTACGAATACGGTCGATCTTCGCCGGCATCGCCt              >  3:214057/1‑149 (MQ=255)
                                                                                                                                       cacaGGAATCA‑CCAGACCTGGAGGTCATGTGATACCTGTGGGAGCGGGCGTGCCCGCGAAGGGCAGCTAGGC‑GGCCCCATTTCAATTTCCGGCTTTGATACTGGTCCAGATTCGCGTACGAATACGGTCGATCTTCGCCGGCATCGCCt              >  5:246703/1‑149 (MQ=255)
                                                                                                                                        acaGGTATCA‑CATGACCTCCAGGTCTGGTGATTCCTGTGGGAGCGGGCGTGCCCGCGAAGGGCCGCTAGGC‑GGCCCCATTTCAATTTCCGGCTTTGATACTGGTCCAGATTCGCGTACGAATACGGTCGATCTTCGCCGGCATCGCCTc             >  7:66843/1‑149 (MQ=25)
                                                                                                                                            gAATCA‑CCAGACCTGGAGGTCATGTGATACCTGTGGGAGCGGGCGTGCCCGCGAAGGGCCGCTAGGC‑GGCCCCATTTCAATTTCCGGCTTTGATACTGGTCCAGATTCGCGTACGAATACGGTCGATCTTCGCCGGCATCGCCTCCAgc         >  2:49370/1‑149 (MQ=255)
                                                                                                                                             taTCA‑CATGACCTCCAGGTCTGGTGATTCCTGTGGGAGCGGGCGTGCCCGCGAAGGGCCGCTAGGC‑GGCCCCATTTCAATTTCCGGCTTTGATACTGGTCCAGATTCGCGTACGAATACGGTCGATCTTCGCCGGCATCGCCTCCAgcg        <  8:66843/148‑1 (MQ=25)
                                                                                                                                             aaTCA‑CCAGACCTGGAGGTCATGTGATACCTGTGGGAGCGGGCGTGCCCGCGAAGGGCCGCTAGGC‑GGCCCCATTTCAATTTCCGGCTTTGATACTGGTCCAGATTCGCGTACGAATACGGTCGATCTTCGCCGGCATCGCCTCCAgcg        >  3:106613/1‑149 (MQ=255)
                                                                                                                                              aTCA‑CCAGACCTGGAGGTCATGTGATACCTGTGGGAGCGGGCGTGCCCGCGAAGGGCCGCTAGGC‑GGCCCCATTTCAATTTCCGGCTTTGATACTGGTCCAGATTCGCGTACGAATACGGTCGATCTTCGCCGGCATCGCCTCCAgcgc       <  4:214057/149‑1 (MQ=255)
                                                                                                                                                    cAGACCTGGAGGTCATGTGATACCTGTGGGAGCGGGCGTGCCCGCGAAGGGCCGCTAGGC‑GGCCCCATTTCAATTTCCGGCTTTGATACTGGTCCAGATTCGCGTACGAATACGGTCGATCTTCGCCGGCATCGCCTCCAGCGCATACa  >  6:360957/1‑149 (MQ=255)
                                                                                                                                                     |                                                                                                                                                    
ACCTGGCGCCGACCCGGGTCAACCTGGCGCTGATGGAAGAGCGCTGGCCGGACATCAAGTTCCGCGCTACCCGCGAGCACCATTAAGGCCTGAAATCGGGGCTGCTTTGCAGCCCTTCGCGGGCACGCCCGCTCCCACAGGAATCA‑CCAGACCTGGAGGTCATGTGATACCTGTGGGAGCGGGCGTGCCCGCGAAGGGCCGCTAGGC‑GGCCCCATTTCAATTTCCGGCTTTGATACTGGTCCAGATTCGCGTACGAATACGGTCGATCTTCGCCGGCATCGCCTCCAGCGCATACA  >  NC_002947/1013087‑1013382

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑