Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 1,013,241 G→C 11.1% intergenic (+69/+62) prfC → / ← potF‑I peptide chain release factor 3/putrescine‑binding protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029471,013,2410GC11.1% 91.8 / 5.2 36intergenic (+69/+62)prfC/potF‑Ipeptide chain release factor 3/putrescine‑binding protein
Reads supporting (aligned to +/- strand):  ref base G (17/15);  new base C (2/2);  total (19/17)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.70e-01

ACCCGGGTCAACCTGGCGCTGATGGAAGAGCGCTGGCCGGACATCAAGTTCCGCGCTACCCGCGAGCACCATTAAGGCCTGAAATCGGGGCTGCTTTGCAGCCCTTCGCGGGCACGCCCGCTCCCACAGGAATCA‑CCAGACCTGGAGGTCATGTGATACCTGTGGGAGCGGGCGTGCCCGCGAAGGGCCGCTAGGC‑GGCCCCATTTCAATTTCCGGCTTTGATACTGGTCCAGATTCGCGTACGAATACGGTCGATCTTCGCCGGCATCGCCTCCAGCGCATACAGCTTG  >  NC_002947/1013098‑1013387
                                                                                                                                                |                                                                                                                                                   
aCCCGGGTCAACCTGGCGCTGATGGAAGAGCGCTGGCCGGACATCAAGTTCCGCGCTACCCGCGAGCACCATTAAGGCCTGAAATCGGGGCTGCTTTGCAGCCCTTCGCGGGCACGCCCGCTCCCACAGGTATCA‑CATGACCTCCAGGt                                                                                                                                                >  4:233471/1‑149 (MQ=38)
   cGGGTCAACCTGGCGCTGATGGAAGAGCGCTGGCCGGACATCAAGTTCCGCGCTACCCGCGAGCACCATTAAGGCCTGAAATCGGGGCTGCTTTGCAGCCCTTCGCGGGCACGCCCGCTCCCACAGGAATCA‑CCAGACCTGGAGGTCAt                                                                                                                                             <  7:243555/149‑1 (MQ=255)
         aaCCTGGCGCTGATGGAAGAGCGCTGGCCGGACATCAAGTTCCGCGCTACCCGCGAGCACCATTAAGGCCTGAAATCGGGGCTGCTTTGCAGCCCTTCGCGGGCACGCCCGCTCCCACAGGAATCA‑CCAGACCTGGAGGTCATGTGATa                                                                                                                                       >  1:316936/1‑149 (MQ=255)
               gcgcTGATGGAAGAGCGCTGGCCGGACATCAAGTTCCGCGCTACCCGCGAGCACCATTAAGGCCTGAAATCGGGGCTGCTTTGCAGCCCTTCGCGGGCACGCCCGCTCCCACAGGAATCA‑CCAGACCTGGAGGTCATGTGATACCtg                                                                                                                                   <  2:316936/147‑1 (MQ=255)
                cgcTGATGGAAGAGCGCTGGCCGGACATCAAGTTCCGCGCTACCCGCGAGCACCATTAAGGCCTGAAATCGGGGCTGCTTTGCAGCCCTTCGCGGGCACGCCCGCTCCCACAGGTATCA‑CATGACCTCCAGGTCTGGTGATTCCTGTgg                                                                                                                                <  3:233471/149‑1 (MQ=25)
                    gATGGAAGAGCGCTGGCCGGACATCAAGTTCCGCGCTACCCGCGAGCACCATTAAGGCCTGAAATCGGGGCTGCTTTGCAGCCCTTTGCGGGCACGCCCGCTCCCACAGGAATCA‑CCAGACCTGGAGGTCAtgt                                                                                                                                           >  6:188781/1‑134 (MQ=255)
                    gATGGAAGAGCGCTGGCCGGACATCAAGTTCCGCGCTACCCGCGAGCACCATTAAGGCCTGAAATCGGGGCTGCTTTGCAGCCCTTTGCGGGCACGCCCGCTCCCACAGGAATCA‑CCAGACCTGGAGGTCAtgt                                                                                                                                           <  5:188781/134‑1 (MQ=255)
                       ggAAGAGCGCTGGCCGGACATCAAGTTCCGCGCTACCCGCGAGCACCATTAAGGCCTGAAATCGGGGCTGCTTTGCAGCCCTTCGCGGGCCCGCCCCCTCCCACACGAAACA‑CCACACCAGGTGGTCATGTGTTAACGGTTGGGGCggg                                                                                                                         >  5:31397/1‑149 (MQ=37)
                          cccgatcTGGCCGGACATCAAGTTCCGCGCTACCCGCGAGCACCATTAAGGCCTGAAATCGGGGCTGCTTTGCAGCCCTTCGCGGGCACGCCCGCTCCCACAGGAATCACCCAGACCTGGAGGTCATGTGATACCTGTGGGAGCgg                                                                                                                          <  1:53092/140‑1 (MQ=255)
                                   gCCGGACATCAAGTTCCGCGCTACCCGCGAGCACCATTAAGGCCTGAAATCGGGGCTGCTTTGCAGCCCTTCGCGGGCACGCCCGCTCCCACAGGAATCA‑CCAGACCTGGAGGTCATGTGATACCTGTGGGAGCgg                                                                                                                          >  2:53092/1‑136 (MQ=255)
                                    ccGGACATCAAGTTCCGCGCTACCCGCGAGCACCATTAAGGCCTGAAATCGGGGCTGCTTTGCAGCCCTTCGCGGGCACGCCCGCTCCCACAGGAATCA‑CCAGACCTGGAGGTCAtgt                                                                                                                                           >  1:305912/1‑118 (MQ=255)
                                    ccGGACATCAAGTTCCGCGCTACCCGCGAGCACCATTAAGGCCTGAAATCGGGGCTGCTTTGCAGCCCTTCGCGGGCACGCCCGCTCCCACAGGAATCA‑CCAGACCTGGAGGTCAtgt                                                                                                                                           <  2:305912/118‑1 (MQ=255)
                                                           ccGCGAGCACCATTAAGGCCTGAAATCGGGGCTGCTTTGCAGCCCTTCGCGGGCACGCCCGCTCCCACAGGAATCA‑CCAGACCTGGAGGTCATGTTATACCTGTGGGAGCGGGCGTGCCCGCGAAg                                                                                                            <  4:256615/126‑1 (MQ=255)
                                                           ccGCGAGCACCATTAAGGCCTGAAATCGGGGCTGCTTTGCAGCCCTTCGCGGGCACGCCCGCTCCCACAGGAATCA‑CCAGACCTGGAGGTCATGTGATACCTGTGGGAGCGGGCGTGCCCGCGAAg                                                                                                            >  3:256615/1‑126 (MQ=255)
                                                                     cATTAAGGCCTGGAATCGGGGCTGCTTTGCAGCCCTTCGCGGGCCCGCCCCCACCCCCCAGAAAAA‑CCACAACAGGAGGGCATGTGATAACAGTGGGAGCGGGGGTGCCCGCGAAGGGGCCCGAGGCGGGCCCCATTTAAAtttaggcg                                                                           >  8:303773/1‑144 (MQ=255)
                                                                                gAAATCGGGGCTGCTTTGCAGCCCTTCGCGGGCACGCCCGCTCCCACAGGAATCA‑CCAGACCTGGAGGTCATGTGAt                                                                                                                                        >  8:221939/1‑77 (MQ=255)
                                                                                gAAATCGCGGCTGCTTTGCATCCCTTCGCGGGGCCGCCCGCTCCCACACGAATCC‑CCAGACCTGGAGGTCATGTGAt                                                                                                                                        <  7:221939/77‑1 (MQ=255)
                                                                                          ctgctTTGCAGCCCTTCGCGGCCACCCCCGCTCCCACAGGAATCA‑CCACCCCTGGAGGTAATGTTATACCTGTGGGAGCGGGCGTGCCCGCGAAGGGCCGCTAGGC‑GGCCCCATTTCAATTTCCGGCTTTGATACTGGTCCAGATTcgc                                                     <  7:303773/149‑1 (MQ=38)
                                                                                               ttGCCGCCCTTCGCGGGCACGCCCGCTCCCACAGGAATCA‑CCAGACCTGGAGGTCATGTGATACCTGTGGGAGCGGGCGTGCCCGCGAAGGGCCGCTAGGC‑GGCCCCATTTCAATTTcc                                                                              <  7:327006/119‑1 (MQ=255)
                                                                                                     cccTGCGGGGCCCCGCCCGCCCCCACCGCATTCC‑CCCGCCCTGTTGGTCTTGTTATCCGGGTGGGAGGGGGGGTGCCCGCGAAGGGCCGCTAGGC‑GGCCCCATTTCAATTTCCGGCTTTGATACTGGTCCAGATTCGCGTACGAATACg                                          <  6:31397/149‑1 (MQ=255)
                                                                                                                   gCCCGCTCCCACAGGAATCA‑CCAGACCTGGAGGTCATGTGATACCTGTGGGAGCGGGCGTGCCCGCGAAGGGCCGCTAGGC‑GGCCCCATTTCAATTTCCGGCTTTGATACTGGTCCAGATTCGCGTATGAATACGGTCGATCTTCGCCg                            >  1:141677/1‑149 (MQ=255)
                                                                                                                            cacaGGAATCA‑CCAGACCTGGAGGTCATGTGATACCTGTGGGAGCGGGCGTGCCCGCGAAGGGCCGCTAGGC‑GGCCCCATTTCAATTTCCGGCTTTGATACTGGTCCAGATTCGCGTACGAATACGGTCGATCTTc                                >  8:201182/1‑136 (MQ=255)
                                                                                                                            cacaGGAATCA‑CCAGACCTGGAGGTCATGTGATACCTGTGGGAGCGGGCGTGCCCGCGAAGGGCCGCTAGGC‑GGCCCCATTTCAATTTCCGGCTTTGATACTGGTCCAGATTCGCGTACGAATACGGTCGATCTTc                                <  7:201182/136‑1 (MQ=255)
                                                                                                                            cacaGGAATCA‑CCAGACCTGGAGGTCATGTGATACCTGTGGGAGCGGGCGTGCCCGCGAAGGGCCGCTAGGC‑GGCCCCATTTCAATTTCCGGCTTTGATACTGGTCCAGATTCGCGTACGAATACGGTCGATCTTCGCCGGCATCGCCt                   >  8:96954/1‑149 (MQ=255)
                                                                                                                            cacaGGAATCA‑CCAGACCTGGAGGTCATGTGATACCTGTGGGAGCGGGCGTGCCCGCGAAGGGCCGCTAGGC‑GGCCCCATTTCAATTTCCGGCTTTGATACTGGTCCAGATTCGCGTACGAATACGGTCGATCTTCGCCGGCATCGCCt                   >  3:214057/1‑149 (MQ=255)
                                                                                                                            cacaGGAATCA‑CCAGACCTGGAGGTCATGTGATACCTGTGGGAGCGGGCGTGCCCGCGAAGGGCAGCTAGGC‑GGCCCCATTTCAATTTCCGGCTTTGATACTGGTCCAGATTCGCGTACGAATACGGTCGATCTTCGCCGGCATCGCCt                   >  5:246703/1‑149 (MQ=255)
                                                                                                                             acaGGTATCA‑CATGACCTCCAGGTCTGGTGATTCCTGTGGGAGCGGGCGTGCCCGCGAAGGGCCGCTAGGC‑GGCCCCATTTCAATTTCCGGCTTTGATACTGGTCCAGATTCGCGTACGAATACGGTCGATCTTCGCCGGCATCGCCTc                  >  7:66843/1‑149 (MQ=25)
                                                                                                                                 gAATCA‑CCAGACCTGGAGGTCATGTGATACCTGTGGGAGCGGGCGTGCCCGCGAAGGGCCGCTAGGC‑GGCCCCATTTCAATTTCCGGCTTTGATACTGGTCCAGATTCGCGTACGAATACGGTCGATCTTCGCCGGCATCGCCTCCAgc              >  2:49370/1‑149 (MQ=255)
                                                                                                                                  taTCA‑CATGACCTCCAGGTCTGGTGATTCCTGTGGGAGCGGGCGTGCCCGCGAAGGGCCGCTAGGC‑GGCCCCATTTCAATTTCCGGCTTTGATACTGGTCCAGATTCGCGTACGAATACGGTCGATCTTCGCCGGCATCGCCTCCAgcg             <  8:66843/148‑1 (MQ=25)
                                                                                                                                  aaTCA‑CCAGACCTGGAGGTCATGTGATACCTGTGGGAGCGGGCGTGCCCGCGAAGGGCCGCTAGGC‑GGCCCCATTTCAATTTCCGGCTTTGATACTGGTCCAGATTCGCGTACGAATACGGTCGATCTTCGCCGGCATCGCCTCCAgcg             >  3:106613/1‑149 (MQ=255)
                                                                                                                                   aTCA‑CCAGACCTGGAGGTCATGTGATACCTGTGGGAGCGGGCGTGCCCGCGAAGGGCCGCTAGGC‑GGCCCCATTTCAATTTCCGGCTTTGATACTGGTCCAGATTCGCGTACGAATACGGTCGATCTTCGCCGGCATCGCCTCCAgcgc            <  4:214057/149‑1 (MQ=255)
                                                                                                                                         cAGACCTGGAGGTCATGTGATACCTGTGGGAGCGGGCGTGCCCGCGAAGGGCCGCTAGGC‑GGCCCCATTTCAATTTCCGGCTTTGATACTGGTCCAGATTCGCGTACGAATACGGTCGATCTTCGCCGGCATCGCCTCCAGCGCATACa       >  6:360957/1‑149 (MQ=255)
                                                                                                                                            aCCTGGAGGGCATGTGATACCTGTGGGAGCGGGCGTGCCCGCGAAGGGCCGCTAGGC‑GGCCCCATTTCAATTTCCGGCTTTGATACTGGTCCAGATTCGCGTATGAATACGGTCGATCTTCGCCGGCATCGCCTCCAGCGCATACAGCt    <  2:141677/149‑1 (MQ=255)
                                                                                                                                              cTGGAGGTCATGTGATACCTGTGGGAGCGGGCGTGCCCGCGAAGGGCCGCTAGGC‑GGCCCCATTTCAATTTCCGGCTTTGATACTGGTCCAGATTCGCGTACGAATACGGTCGATCTTCGCCGGCATCGCCTCCAGCGCATACAGCTTg  <  4:106613/149‑1 (MQ=255)
                                                                                                                                               tGGAGGTCATGTGATACCTGTGGGAGCGGGCGTGCCCGCGAAGGGCCGCTAGGC‑GGCCCCATTTCAATTTCCGGCTTTGATACTGGTCCAGATTCGCGTACGAATACGGTCGATCTTCGCCGGc                          >  3:21844/1‑124 (MQ=255)
                                                                                                                                               tGGAGGTCATGTGATACCTGTGGGAGCGGGCGTGCCCGCGAAGGGCCGCTAGGC‑GGCCCCATTTCAATTTCCGGCTTTGATACTGGTCCAGATTCGCGTACGAATACGGTCGATCTTCGCCGGc                          <  4:21844/124‑1 (MQ=255)
                                                                                                                                                |                                                                                                                                                   
ACCCGGGTCAACCTGGCGCTGATGGAAGAGCGCTGGCCGGACATCAAGTTCCGCGCTACCCGCGAGCACCATTAAGGCCTGAAATCGGGGCTGCTTTGCAGCCCTTCGCGGGCACGCCCGCTCCCACAGGAATCA‑CCAGACCTGGAGGTCATGTGATACCTGTGGGAGCGGGCGTGCCCGCGAAGGGCCGCTAGGC‑GGCCCCATTTCAATTTCCGGCTTTGATACTGGTCCAGATTCGCGTACGAATACGGTCGATCTTCGCCGGCATCGCCTCCAGCGCATACAGCTTG  >  NC_002947/1013098‑1013387

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑