Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 1,026,484 G→C 10.3% L162V (CTG→GTG)  dppA‑II ← dipeptide ABC transporter substrate‑binding protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029471,026,4840GC10.3% 134.0 / 5.6 48L162V (CTG→GTG) dppA‑IIdipeptide ABC transporter substrate‑binding protein
Reads supporting (aligned to +/- strand):  ref base G (22/21);  new base C (3/2);  total (25/23)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.10e-01

GAACACGAACGGCCCGGTACCGATAGGCTGCTGATTGATGTCGCTGGGGCGTCCACTGGCCAGTAGCTGCTCGGCGTATTCAGCGGAGAGGATGGAGGCGAAGCTCATGGCCAGGTTCTGGATGAACGCGGCATCGACGGTGTTCAGGGTGAACACCACGGTCAATGGGTCGGTCTTCTCGACACGGGCGATGTTCTTGTCCAGGCCCATGCCGTTGAAATAGGGAAACTCGGTAGGGTAGGCCTGACGGAACCGATGACCCTTGTCGAGCATGCGGTTGAAGGTGAAC  >  NC_002947/1026338‑1026626
                                                                                                                                                  |                                                                                                                                              
gAACACGAACGGCCCTGTACCGATAGGCTGCTGATTGATGTCGCTGGGGCGTCCACTGGCCAGTAGCTGCTCGGCGTATTCAGCGGAGAGGATGGAGGAGAAGCTCATGGCCAGGTTCTGGATGAACGCGGCATCGACGGTGTTCAg                                                                                                                                                <  5:297005/147‑1 (MQ=255)
   cacGAACGGCCCGGTACCGATAGGCTGCTGATTGATGTCGCTGGGGCGTCCACTGGCCAGTAGCTGCTCGGCGTATTCAGCGGAGAGGATGGAGGCGAAGCTCATGGCCAGGTTCTGGATGAACGCGGCATCGACGGTGTTCAGGGTg                                                                                                                                            >  7:292736/1‑148 (MQ=255)
    acGAACGGCCCGGTAACGATAGGCTTCTGATTGATTTGGCTGGGGCGTCCACTGGCCAGTAGCTGCTCGGCGTATTGAGCGGAGAGGATGGAGGCGAAGCTCATGGCCAGGTTCTGGATGAACGCGGCATCGACTGTGTTCAGGGTGaa                                                                                                                                          <  4:69162/149‑1 (MQ=38)
     cGAACGGCCCGGTACCGATAGGCTGCTGATTGATGTCGCTGGGGCGTCCACTGGCCAGTAGCTGCTCGGCGTATTCAGCGGAGAGGATGGAGGCGAAGCTCATGGCCAGGTTCTGGATGAACGCGGCATCGACGGTGTTCAGGGTGAac                                                                                                                                         <  8:292736/149‑1 (MQ=255)
                   ccGATAGGCTGCTGATTGATGTCGCTGGGGCGTCCACTGGCCAGTAGCTGCTCGGCGTATTCAGCGGAGAGGATGGAGGCGAAGCTCATGGCCAGGTTCTGGATGAACGCGGCATCGACGGTGTTCAGGGTGAACACCACGGTCAATgg                                                                                                                           <  1:227352/149‑1 (MQ=255)
                           ctgctgATTGATGTCGCTGGGGCGTCCACTGGCCAGTAGCTGCTCGGCGTATTCAGCGGAGAGGATGGAGGCGAAGCTCATGGCCAGGTTCTGGATGAACGCGGCATCGACGGTGTTCAGGGTGAACACCACGGTCAATGggt                                                                                                                         <  4:56200/143‑1 (MQ=255)
                           ctgctgATTGATGTCGCTGGGGCGTCCACTGGCCAGTAGCTGCTCGGCGTATTCAGCGGAGAGGATGGAGGCGAAGCTCATGGCCAGGTTCTGGATGAACGCGGCATCGACGGTGTTCAGGGTGAACACCACGGTCAATGggt                                                                                                                         >  3:56200/1‑143 (MQ=255)
                           ctgctgATTGATGTCGCTGGGGCGTCCACTGGCCAGTAGCTGCTCGGCGTATTCAGCGGAGAGGATGGAGGCGAAGCTCATGGCCAGGTTCTGGATGAACGCGGCATCGACGGTGTTCAGGGTGAACACCACGGTCAATGGGTCGGtct                                                                                                                   >  8:254217/1‑149 (MQ=255)
                                 attgatGTCGCTGGGGCGTCCACTGGCCAGTAGCTGCTCGGCGTATTCAGCGGAGAGGATGGAGGCGAAGCTCATGGCCAGGTTCTGGATGAACGCGGCATCGACGGTGTTCACCCTGAAcacc                                                                                                                                      <  5:358359/124‑1 (MQ=255)
                                 attgatGTCGCTGGGGCGTCCACTGGCCAGTAGCTGCTCGGCGTATTCAGCGGAGAGGATGGAGGCGAAGCTCATGGCCAGGTTCTGGATGAACGCGGCATCGACGGTGTTCACCCTGAAcacc                                                                                                                                      >  6:358359/1‑124 (MQ=255)
                                     atGTCGCTGGGGCGTCCACTGGCCAGTAGCTGCTCGGCGTATTCAGCGGAGAGGATGGAGGCGAAGCTCATGGCCAGGTTCTGGATGAACGCGGCATCGACGGTGTTCAGGGTGAACACCACGGTCAATGGGTCGGTCTTCTCGACACg                                                                                                         >  4:9452/1‑149 (MQ=255)
                                           cTGGGGCGTCCACTGGCCAGTAGCTGCTCGGCGTATTCAGCGGAGAGGATGGAGGCGAAGCTCATGGCCAGGTTCTGGATGAACGCGGCATCGACGGTGTTAAGGGTGAACACCACGGTCAATgg                                                                                                                           >  4:153554/1‑125 (MQ=255)
                                              gggCGTCCACTGGCCAGTAGCTGCTCGGCGTATTCAGCGGAGAGGATGGAGGCGAAGCTCATGGCCAGGTTCTGGATGAACGCGGCATCGACGGTGTTCAGGGTGAACACCACGGTCAATGGGTCGGTCTTCTCGACACGGGCGATGtt                                                                                                >  2:81146/1‑149 (MQ=255)
                                                 cGTCCACTGGCCAGTAGCTGCTCGGCGTATTCAGCGGAGAGGATGGAGGCGAAGCTCATGGCCAGGTTCTGGATGAACGCGGCATCGACGGTGTTCAGGGTGAACACCACGGTCAATGGGTCGGTCTTCTCGACAGGGGCGATGTTCtt                                                                                             <  3:9452/149‑1 (MQ=255)
                                                          gCCAGTAGCTGCTCGGCGTATTCAGCGGAGAGGATGGAGGCGAAGCTCATGGCCAGGTTCTGGATGAACGCGGCATCGACGGTGTTCAGGGTGAACACCACGGTCAATGGGTCGGTCTTCTCGACACGGGCGATGTTCTTGTCCAGGc                                                                                     <  3:202734/148‑1 (MQ=255)
                                                           ccAGTAGCTGCTCGGCGTATTCAGCGGAGAGGATGGAGGCGAAGCTCATGGCCAGGTTCTGGATGAACGCGGCATCGACGGTGTTCAGGGTGAACACCACGGTCAATGGGTCGGTCTTCTCGACACGGGCGATGTTCTTGTCCAGGccc                                                                                   <  6:179963/149‑1 (MQ=255)
                                                            cAGTAGCTGCTCGGCGTATTCAGCGGAGAGGATGGAGGCGAAGCTCATGGCCAGGTTCTGGATGAACGCGGCATCGACGGTGTTCAGGGTGAACACCACGGTCAATGGGTCGGTCTTCTCGACACGGGCGATGTTCTTGTCCAGGCCCa                                                                                  >  4:162043/1‑149 (MQ=255)
                                                                       cGGCGTATTCAGCGGAGAGGATGGAGGCGAAGCTCATGGCCAGGTTCTGGATGAACGCGGCATCGACGGTGTTCAGGGTGAACACCACGGTCAATGGGTCGGTCTTCTCGACACGGGCGATGTTCTTGTCAAGGCCCATGCCCTTTaaa                                                                       >  8:319196/1‑149 (MQ=255)
                                                                        ggCGTATTCAGCGGAGAGGATGGAGGCGAAGCTCATGGCGAGGTTCTGGATGAACGCGGCATCGACGGTGTTCAGGGTGAACACCACGGTCAATGGGTCGGTCTTCTCGACACGGGCGATGTTCTTGTCCAGGCCCATGCCGTTGaaa                                                                       <  7:319196/148‑1 (MQ=255)
                                                                           gTATTCAGCGGAGAGGATGGAGGCGAAGCTCATGGCCAGGTTCTGGATGAACGCGGCATCGACGGTGTTCAGGGTGAACACCACGGTCAATGGGTCGGTCTTCTCGACACGGGCGATGTTCTTGTCCAGGCCCATGCCGTTGAAATAgg                                                                   <  1:81146/149‑1 (MQ=255)
                                                                            tATTCAGCGGAGAGGATGGAGGCAAAGCTCATGGCCAGGTTCTGGATGAACGCGGCATCGACGGTGTTCAGGGTGAACACCACGGTCAATGGGTCGGTCTTCTCGACACGGGCGATGTTCtt                                                                                             >  6:228207/1‑122 (MQ=255)
                                                                            tATTCAGCGGAGAGGATGGAGGCAAAGCTCATGGCCAGGTTCTGGATGAACGCGGCATCGACGGTGTTCAGGGTGAACACCACGGTCAATGGGTCGGTCTTCTCGACACGGGCGATGTTCtt                                                                                             <  5:228207/122‑1 (MQ=255)
                                                                                   cGGAGAGGATGGAGGCGAAGCTCATGGCCAGGTTCTGGATGAACGCGGCATCGACGGTGTTCAGGGTGAACACCACGGTCAATGGGTCGGTCTTCTCGACACGGGCGATGTTCTTGTCCAGGCCCATGCCGTTGAAATAGGGAAACTCg                                                           >  5:309459/1‑149 (MQ=255)
                                                                                           aTGGAGGCGAAGCTCATGGCCAGGTTCTGGATGAACGCGGCATCGACGGTGTTCAGGGTGAACACCACGGTCAATGGGTCGGTCTTCTCGACACGGGCGATGTTCTTGTCCAGGCCCATGCCGTTGAAATAGGGAAACTCggtagggta                                                   <  7:254217/149‑1 (MQ=255)
                                                                                           aTGGAGGCGAAGCTCATGGCCAGGTTCTGGATGAACGCGGCATCGACGGTGTTCAGGGTGAACACCACGGTCAATGGGTCGGTCTTCTCGACACGGGCGATGTTCTTGTCCAGGCCCATGCCGTTGAAATAGGGAAACTCggtagggta                                                   >  8:296374/1‑149 (MQ=255)
                                                                                                ggCGAAGCTCATGGCCAGGTTCTGGATGAACGCGGCATCGACGGTGTTCAGGGTGAACACCACGGTGAATGGGTCGGTCTGCTCGCCACGGGCGATGTTCTTGTCCAGGCAGAGGCGGTTGAAATAGGGAAACTCGTTAGGGTAGGGCt                                              >  8:154709/1‑149 (MQ=38)
                                                                                                 gCGAAGCTCATGGCCAGGTTCTGGATGAACGCGGCATCGACGGTGTTCAGGGTGAACACCACGGTCAATGGGTCGGTCTTCTCGACACGGGCGATGTTCTTGTCCAGGCCCATGCCGTTGAAATAGGGAAACTCGGTAGGGTAGGCCt                                              >  5:193796/1‑148 (MQ=255)
                                                                                                   gAAGCTCATGGCCAGGTTCTGGATGAACGCGGCATCGACGGTGTTCAGGGTGAACACCACGGTCAATGGGTCGGTCTTCTCGACACGGGCGATGTTCTTGTCCAGGCCCATGCCGTTGAAATAGGGAAACTCGGTAGGGTAGGCCTGAc                                           <  3:162043/149‑1 (MQ=255)
                                                                                                        tCATGGCCAGGTTCTGGATGAACGCGGCATCGACGGTGTTCAGGGTGAACACCACGGTCAATGGGTCGGTCTTCTCGACACGGGCGATGTTCTTGTCCAGGCCCATGCCGTTGAAATAGGGAAACTCGGTAGGGTAGGCCTGACGGAAc                                      >  4:91415/1‑149 (MQ=255)
                                                                                                          aTGGCCAGGTTCTGGATGAACGCGGCATCGACGGTGTTCAGGGTGAACACCACGGTCAATGGGTCGGTCTTCTCGACACGGGCGATGTTCTTGTCCAGGCCCATGCCGTTGAAATAGGGAAACTCGGTAGGGTAGGCCTGACGGAAc                                      <  2:109422/147‑1 (MQ=255)
                                                                                                          aTGGCCAGGTTCTGGATGAACGCGGCATCGACGGTGTTCAGGGTGAACACCACGGTCAATGGGTCGGTCTTCTCGACACGGGCGATGTTCTTGTCCAGGCCCATGCCGTTGAAATAGGGAAACTCGGTAGGGTAGGCCTGACGGAAc                                      <  3:91415/147‑1 (MQ=255)
                                                                                                               cAGGTTCTGGATGAACGCGGCATCGACGGTGTTCAGGGTGAACACCACGGTCAATGGGTCGGTCTTCTCGACACGGGCGATGTTCTTGTCCAGGCCCATGCCGTTGAAATAGGGAAACTCGGTAGGGTAGGCCTGACGGAACCGATGAc                               >  5:171830/1‑149 (MQ=255)
                                                                                                                   ttCTGGATGAACGCGGCATCGACGGTGTTCAGGGTGAACACCACGGTCAATGGGTCGGTCTTCTCGACACGGGCGATGTTCTTGTCCAGGCCCATGCCGTTGAAATAGGGAAACTCGGTAGGGTAGGCCTGACGGAACCGATGACCCtt                           >  6:292146/1‑149 (MQ=255)
                                                                                                                       ggATGAACGCGGCATCGACGGTGTTCAGGGTGAACACCACGGTCAATGGGTCGGTCTTCTCGACACGGGCGATGTTCTTGTCCAGGCCCATGCCGTTGAAATAGGGAAACTCGGTAGGGTAGGCCTGACGGAAACGATGACCCTTGTc                        >  4:293548/1‑148 (MQ=255)
                                                                                                                        gATGAACGCGGCATCGACGGTGTTCAGGGTGAACACCACGGTCAATGGGTCTGTCTTCTCGACACGGGCGATGTTCTTGTCCAGGCCCATGCCGTTGAAATAGGGAAACTCggtaggg                                                     <  8:20734/118‑1 (MQ=255)
                                                                                                                        gATGAACGCGGCATCGACGGTGTTCAGGGTGAACACCACGGTCAATGGGTCGGTCTTCTCGACACGGGCGATGTTCTTGTCCAGGCCCATGCCGTTGAAATAgg                                                                   <  1:327596/104‑1 (MQ=255)
                                                                                                                        gATGAACGCGGCATCGACGGTGTTCAGGGTGAACACCACGGTCAATGGGTCGGTCTTCTCGACACGGGCGATGTTCTTGTCCAGGCCCATGCCGTTGAAATAgg                                                                   >  2:327596/1‑104 (MQ=255)
                                                                                                                        gATGAACGCGGCATCGACGGTGTTCAGGGTGAACACCACGGTCAATGGGTCGGTCTTCTCGACACGGGCGATGTTCTTGTCCAGGCCCATGCCGTTGAAATAGGGAAACTCggtaggg                                                     >  7:20734/1‑118 (MQ=255)
                                                                                                                        gATGAACGCGGCATCGACGGTGTTCAGGGTGAACACCACGGTCAATGGGTCGGTCTTCTCGACACGGGCGATGTTCTTGTCCAGGCCCATGCCGTTGAAATAGGGAAACTCGGTAGGGTAGGCCTGACGGAACCGATGACCCTTGTc                        >  1:269115/1‑147 (MQ=255)
                                                                                                                          tGAACGCGGCATCGACGGTGTTCAGGGTGAACACCACGGTCAATGGGTCGGTCTTCTCGACACGGGCGATGTTCTTGTCCAGGCCCATGCCg                                                                             <  4:131799/92‑1 (MQ=255)
                                                                                                                          tGAACGCGGCATCGACGGTGTTCAGGGTGAACACCACGGTCAATGGGTCGGTCTTCTCGACACGGGCGATGTTCTTGTCCAGGCCCATGCCg                                                                             >  3:131799/1‑92 (MQ=255)
                                                                                                                              cgcgGCATCGACGGTGTTCAGGGTGAACACCACGGTCAATGGGTCGGTCTTCTCGACACGGGCGATGTTCTTGTCCAGGCCCATGCCGTTGAAATAGGGAAACTCGGTAGGGTAGGCCTGACGGAACCGATGACCCTTGTCGAGCATGc                <  6:171830/149‑1 (MQ=255)
                                                                                                                                   cATCGACGGTGTTCAGGGTGAACACCACGGTCAATGGGTCGGTCTTCTCGACACGGGCGATGTTCTTGTCCAGGCCCATGCCGTTGAAATAGGGAAACTCGGTAGGGTAGGCCTGACGGAACCGATGACCCTTGTCGAGCATGCGGtt            >  3:41731/1‑148 (MQ=255)
                                                                                                                                     tCGACGGTGTTCAGGGTGAACACCACGGTCAATGGGTCGGTCTTCTCGACACGGGCGATGTTCTTGTCCAGGCCCATGCCGTTGAAATAGGGAAACTCGGTAGGGTAGGCCTGACGGAACCGATGACCCTTGTCGAGCATGCGGTTGaa         <  5:292146/149‑1 (MQ=255)
                                                                                                                                       cgtggTGTTCACCCTGAACACCGCGGTCAATGGGTCGGTCTTCTCGACACGGGCGATGTTCTTGTCCAGGCCCATGCCGTTGAAATAGGGAAACTCGGTAGGGTAGGCCTGACGGAACCGATGACCCTTGTCGAGCATGCGGTTGAAgg       >  5:202070/4‑149 (MQ=38)
                                                                                                                                        gtggTGTTCACCCTGAACACCACGGTCAATGGGTCGGTCTTCTCGACACGGGCGATGTTCTTGTCCAGGCCCATGCCGTTGAAATAGGGAAACTCGGTAGGGTAGGCCTGACGGaa                                       >  3:60378/3‑116 (MQ=255)
                                                                                                                                        gtggTGTTCACCCTGAACACCACGGTCAATGGGTCGGTCTTCTCGACACGGGCGATGTTCTTGTCCAGGCCCATGCCGTTGAAATAGGGAAACTCGGTAGGGTAGGCCTGACGGaa                                       <  4:60378/114‑1 (MQ=255)
                                                                                                                                           gtgtTCAGGGTGAACACCACGGTCAATGGGTCGGTCTTCTCGACACGGGCGATGTTCTTGTCCAGGCCCATGCCGTTGAAATAGGGAAACTCGGTAGGGTAGGCCTGACGGAACCGATGACCCTTGTCGAGCATGCGGTTGAAGGTGaa   <  7:296374/149‑1 (MQ=255)
                                                                                                                                            tgtTCAGGGTGAACACCACGGTCAATGGGTCGGTCTTCTCGACACGGGCGATGTTCTTGTCCAGGCCCATGCCGTTGAAATAGGGAAACTCGGTAGGGTAGGCCTGACGGAACCGATGACCCTTGTCGAGCATGCGGTTGAAGGTGAAc  <  6:309459/149‑1 (MQ=255)
                                                                                                                                                  |                                                                                                                                              
GAACACGAACGGCCCGGTACCGATAGGCTGCTGATTGATGTCGCTGGGGCGTCCACTGGCCAGTAGCTGCTCGGCGTATTCAGCGGAGAGGATGGAGGCGAAGCTCATGGCCAGGTTCTGGATGAACGCGGCATCGACGGTGTTCAGGGTGAACACCACGGTCAATGGGTCGGTCTTCTCGACACGGGCGATGTTCTTGTCCAGGCCCATGCCGTTGAAATAGGGAAACTCGGTAGGGTAGGCCTGACGGAACCGATGACCCTTGTCGAGCATGCGGTTGAAGGTGAAC  >  NC_002947/1026338‑1026626

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑