Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 1,026,486 G→C 10.4% T161S (ACC→AGC) ‡ dppA‑II ← dipeptide ABC transporter substrate‑binding protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029471,026,4860GC10.4% 141.5 / 7.9 48T161S (ACC→AGC) ‡dppA‑IIdipeptide ABC transporter substrate‑binding protein
Reads supporting (aligned to +/- strand):  ref base G (22/21);  new base C (3/2);  total (25/23)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.67e-01

CACGAACGGCCCGGTACCGATAGGCTGCTGATTGATGTCGCTGGGGCGTCCACTGGCCAGTAGCTGCTCGGCGTATTCAGCGGAGAGGATGGAGGCGAAGCTCATGGCCAGGTTCTGGATGAACGCGGCATCGACGGTGTTCAGGGTGAACACCACGGTCAATGGGTCGGTCTTCTCGACACGGGCGATGTTCTTGTCCAGGCCCATGCCGTTGAAATAGGGAAACTCGGTAGGGTAGGCCTGACGGAACCGATGACCCTTGTCGAGCATGCGGTTGAAGGTGAAC  >  NC_002947/1026341‑1026626
                                                                                                                                                 |                                                                                                                                            
cacGAACGGCCCGGTACCGATAGGCTGCTGATTGATGTCGCTGGGGCGTCCACTGGCCAGTAGCTGCTCGGCGTATTCAGCGGAGAGGATGGAGGCGAAGCTCATGGCCAGGTTCTGGATGAACGCGGCATCGACGGTGTTCAGGGTg                                                                                                                                            >  7:292736/1‑148 (MQ=255)
 acGAACGGCCCGGTAACGATAGGCTTCTGATTGATTTGGCTGGGGCGTCCACTGGCCAGTAGCTGCTCGGCGTATTGAGCGGAGAGGATGGAGGCGAAGCTCATGGCCAGGTTCTGGATGAACGCGGCATCGACTGTGTTCAGGGTGaa                                                                                                                                          <  4:69162/149‑1 (MQ=38)
  cGAACGGCCCGGTACCGATAGGCTGCTGATTGATGTCGCTGGGGCGTCCACTGGCCAGTAGCTGCTCGGCGTATTCAGCGGAGAGGATGGAGGCGAAGCTCATGGCCAGGTTCTGGATGAACGCGGCATCGACGGTGTTCAGGGTGAac                                                                                                                                         <  8:292736/149‑1 (MQ=255)
                ccGATAGGCTGCTGATTGATGTCGCTGGGGCGTCCACTGGCCAGTAGCTGCTCGGCGTATTCAGCGGAGAGGATGGAGGCGAAGCTCATGGCCAGGTTCTGGATGAACGCGGCATCGACGGTGTTCAGGGTGAACACCACGGTCAATgg                                                                                                                           <  1:227352/149‑1 (MQ=255)
                        ctgctgATTGATGTCGCTGGGGCGTCCACTGGCCAGTAGCTGCTCGGCGTATTCAGCGGAGAGGATGGAGGCGAAGCTCATGGCCAGGTTCTGGATGAACGCGGCATCGACGGTGTTCAGGGTGAACACCACGGTCAATGggt                                                                                                                         <  4:56200/143‑1 (MQ=255)
                        ctgctgATTGATGTCGCTGGGGCGTCCACTGGCCAGTAGCTGCTCGGCGTATTCAGCGGAGAGGATGGAGGCGAAGCTCATGGCCAGGTTCTGGATGAACGCGGCATCGACGGTGTTCAGGGTGAACACCACGGTCAATGggt                                                                                                                         >  3:56200/1‑143 (MQ=255)
                        ctgctgATTGATGTCGCTGGGGCGTCCACTGGCCAGTAGCTGCTCGGCGTATTCAGCGGAGAGGATGGAGGCGAAGCTCATGGCCAGGTTCTGGATGAACGCGGCATCGACGGTGTTCAGGGTGAACACCACGGTCAATGGGTCGGtct                                                                                                                   >  8:254217/1‑149 (MQ=255)
                              attgatGTCGCTGGGGCGTCCACTGGCCAGTAGCTGCTCGGCGTATTCAGCGGAGAGGATGGAGGCGAAGCTCATGGCCAGGTTCTGGATGAACGCGGCATCGACGGTGTTCACCCTGAAcacc                                                                                                                                      <  5:358359/124‑1 (MQ=255)
                              attgatGTCGCTGGGGCGTCCACTGGCCAGTAGCTGCTCGGCGTATTCAGCGGAGAGGATGGAGGCGAAGCTCATGGCCAGGTTCTGGATGAACGCGGCATCGACGGTGTTCACCCTGAAcacc                                                                                                                                      >  6:358359/1‑124 (MQ=255)
                                  atGTCGCTGGGGCGTCCACTGGCCAGTAGCTGCTCGGCGTATTCAGCGGAGAGGATGGAGGCGAAGCTCATGGCCAGGTTCTGGATGAACGCGGCATCGACGGTGTTCAGGGTGAACACCACGGTCAATGGGTCGGTCTTCTCGACACg                                                                                                         >  4:9452/1‑149 (MQ=255)
                                        cTGGGGCGTCCACTGGCCAGTAGCTGCTCGGCGTATTCAGCGGAGAGGATGGAGGCGAAGCTCATGGCCAGGTTCTGGATGAACGCGGCATCGACGGTGTTAAGGGTGAACACCACGGTCAATgg                                                                                                                           >  4:153554/1‑125 (MQ=255)
                                           gggCGTCCACTGGCCAGTAGCTGCTCGGCGTATTCAGCGGAGAGGATGGAGGCGAAGCTCATGGCCAGGTTCTGGATGAACGCGGCATCGACGGTGTTCAGGGTGAACACCACGGTCAATGGGTCGGTCTTCTCGACACGGGCGATGtt                                                                                                >  2:81146/1‑149 (MQ=255)
                                              cGTCCACTGGCCAGTAGCTGCTCGGCGTATTCAGCGGAGAGGATGGAGGCGAAGCTCATGGCCAGGTTCTGGATGAACGCGGCATCGACGGTGTTCAGGGTGAACACCACGGTCAATGGGTCGGTCTTCTCGACAGGGGCGATGTTCtt                                                                                             <  3:9452/149‑1 (MQ=255)
                                                       gCCAGTAGCTGCTCGGCGTATTCAGCGGAGAGGATGGAGGCGAAGCTCATGGCCAGGTTCTGGATGAACGCGGCATCGACGGTGTTCAGGGTGAACACCACGGTCAATGGGTCGGTCTTCTCGACACGGGCGATGTTCTTGTCCAGGc                                                                                     <  3:202734/148‑1 (MQ=255)
                                                        ccAGTAGCTGCTCGGCGTATTCAGCGGAGAGGATGGAGGCGAAGCTCATGGCCAGGTTCTGGATGAACGCGGCATCGACGGTGTTCAGGGTGAACACCACGGTCAATGGGTCGGTCTTCTCGACACGGGCGATGTTCTTGTCCAGGccc                                                                                   <  6:179963/149‑1 (MQ=255)
                                                         cAGTAGCTGCTCGGCGTATTCAGCGGAGAGGATGGAGGCGAAGCTCATGGCCAGGTTCTGGATGAACGCGGCATCGACGGTGTTCAGGGTGAACACCACGGTCAATGGGTCGGTCTTCTCGACACGGGCGATGTTCTTGTCCAGGCCCa                                                                                  >  4:162043/1‑149 (MQ=255)
                                                                    cGGCGTATTCAGCGGAGAGGATGGAGGCGAAGCTCATGGCCAGGTTCTGGATGAACGCGGCATCGACGGTGTTCAGGGTGAACACCACGGTCAATGGGTCGGTCTTCTCGACACGGGCGATGTTCTTGTCAAGGCCCATGCCCTTTaaa                                                                       >  8:319196/1‑149 (MQ=255)
                                                                     ggCGTATTCAGCGGAGAGGATGGAGGCGAAGCTCATGGCGAGGTTCTGGATGAACGCGGCATCGACGGTGTTCAGGGTGAACACCACGGTCAATGGGTCGGTCTTCTCGACACGGGCGATGTTCTTGTCCAGGCCCATGCCGTTGaaa                                                                       <  7:319196/148‑1 (MQ=255)
                                                                        gTATTCAGCGGAGAGGATGGAGGCGAAGCTCATGGCCAGGTTCTGGATGAACGCGGCATCGACGGTGTTCAGGGTGAACACCACGGTCAATGGGTCGGTCTTCTCGACACGGGCGATGTTCTTGTCCAGGCCCATGCCGTTGAAATAgg                                                                   <  1:81146/149‑1 (MQ=255)
                                                                         tATTCAGCGGAGAGGATGGAGGCAAAGCTCATGGCCAGGTTCTGGATGAACGCGGCATCGACGGTGTTCAGGGTGAACACCACGGTCAATGGGTCGGTCTTCTCGACACGGGCGATGTTCtt                                                                                             <  5:228207/122‑1 (MQ=255)
                                                                         tATTCAGCGGAGAGGATGGAGGCAAAGCTCATGGCCAGGTTCTGGATGAACGCGGCATCGACGGTGTTCAGGGTGAACACCACGGTCAATGGGTCGGTCTTCTCGACACGGGCGATGTTCtt                                                                                             >  6:228207/1‑122 (MQ=255)
                                                                                cGGAGAGGATGGAGGCGAAGCTCATGGCCAGGTTCTGGATGAACGCGGCATCGACGGTGTTCAGGGTGAACACCACGGTCAATGGGTCGGTCTTCTCGACACGGGCGATGTTCTTGTCCAGGCCCATGCCGTTGAAATAGGGAAACTCg                                                           >  5:309459/1‑149 (MQ=255)
                                                                                        aTGGAGGCGAAGCTCATGGCCAGGTTCTGGATGAACGCGGCATCGACGGTGTTCAGGGTGAACACCACGGTCAATGGGTCGGTCTTCTCGACACGGGCGATGTTCTTGTCCAGGCCCATGCCGTTGAAATAGGGAAACTCggtagggta                                                   <  7:254217/149‑1 (MQ=255)
                                                                                        aTGGAGGCGAAGCTCATGGCCAGGTTCTGGATGAACGCGGCATCGACGGTGTTCAGGGTGAACACCACGGTCAATGGGTCGGTCTTCTCGACACGGGCGATGTTCTTGTCCAGGCCCATGCCGTTGAAATAGGGAAACTCggtagggta                                                   >  8:296374/1‑149 (MQ=255)
                                                                                             ggCGAAGCTCATGGCCAGGTTCTGGATGAACGCGGCATCGACGGTGTTCAGGGTGAACACCACGGTGAATGGGTCGGTCTGCTCGCCACGGGCGATGTTCTTGTCCAGGCAGAGGCGGTTGAAATAGGGAAACTCGTTAGGGTAGGGCt                                              >  8:154709/1‑149 (MQ=38)
                                                                                              gCGAAGCTCATGGCCAGGTTCTGGATGAACGCGGCATCGACGGTGTTCAGGGTGAACACCACGGTCAATGGGTCGGTCTTCTCGACACGGGCGATGTTCTTGTCCAGGCCCATGCCGTTGAAATAGGGAAACTCGGTAGGGTAGGCCt                                              >  5:193796/1‑148 (MQ=255)
                                                                                                gAAGCTCATGGCCAGGTTCTGGATGAACGCGGCATCGACGGTGTTCAGGGTGAACACCACGGTCAATGGGTCGGTCTTCTCGACACGGGCGATGTTCTTGTCCAGGCCCATGCCGTTGAAATAGGGAAACTCGGTAGGGTAGGCCTGAc                                           <  3:162043/149‑1 (MQ=255)
                                                                                                     tCATGGCCAGGTTCTGGATGAACGCGGCATCGACGGTGTTCAGGGTGAACACCACGGTCAATGGGTCGGTCTTCTCGACACGGGCGATGTTCTTGTCCAGGCCCATGCCGTTGAAATAGGGAAACTCGGTAGGGTAGGCCTGACGGAAc                                      >  4:91415/1‑149 (MQ=255)
                                                                                                       aTGGCCAGGTTCTGGATGAACGCGGCATCGACGGTGTTCAGGGTGAACACCACGGTCAATGGGTCGGTCTTCTCGACACGGGCGATGTTCTTGTCCAGGCCCATGCCGTTGAAATAGGGAAACTCGGTAGGGTAGGCCTGACGGAAc                                      <  2:109422/147‑1 (MQ=255)
                                                                                                       aTGGCCAGGTTCTGGATGAACGCGGCATCGACGGTGTTCAGGGTGAACACCACGGTCAATGGGTCGGTCTTCTCGACACGGGCGATGTTCTTGTCCAGGCCCATGCCGTTGAAATAGGGAAACTCGGTAGGGTAGGCCTGACGGAAc                                      <  3:91415/147‑1 (MQ=255)
                                                                                                            cAGGTTCTGGATGAACGCGGCATCGACGGTGTTCAGGGTGAACACCACGGTCAATGGGTCGGTCTTCTCGACACGGGCGATGTTCTTGTCCAGGCCCATGCCGTTGAAATAGGGAAACTCGGTAGGGTAGGCCTGACGGAACCGATGAc                               >  5:171830/1‑149 (MQ=255)
                                                                                                                ttCTGGATGAACGCGGCATCGACGGTGTTCAGGGTGAACACCACGGTCAATGGGTCGGTCTTCTCGACACGGGCGATGTTCTTGTCCAGGCCCATGCCGTTGAAATAGGGAAACTCGGTAGGGTAGGCCTGACGGAACCGATGACCCtt                           >  6:292146/1‑149 (MQ=255)
                                                                                                                    ggATGAACGCGGCATCGACGGTGTTCAGGGTGAACACCACGGTCAATGGGTCGGTCTTCTCGACACGGGCGATGTTCTTGTCCAGGCCCATGCCGTTGAAATAGGGAAACTCGGTAGGGTAGGCCTGACGGAAACGATGACCCTTGTc                        >  4:293548/1‑148 (MQ=255)
                                                                                                                     gATGAACGCGGCATCGACGGTGTTCAGGGTGAACACCACGGTCAATGGGTCTGTCTTCTCGACACGGGCGATGTTCTTGTCCAGGCCCATGCCGTTGAAATAGGGAAACTCggtaggg                                                     <  8:20734/118‑1 (MQ=255)
                                                                                                                     gATGAACGCGGCATCGACGGTGTTCAGGGTGAACACCACGGTCAATGGGTCGGTCTTCTCGACACGGGCGATGTTCTTGTCCAGGCCCATGCCGTTGAAATAgg                                                                   <  1:327596/104‑1 (MQ=255)
                                                                                                                     gATGAACGCGGCATCGACGGTGTTCAGGGTGAACACCACGGTCAATGGGTCGGTCTTCTCGACACGGGCGATGTTCTTGTCCAGGCCCATGCCGTTGAAATAgg                                                                   >  2:327596/1‑104 (MQ=255)
                                                                                                                     gATGAACGCGGCATCGACGGTGTTCAGGGTGAACACCACGGTCAATGGGTCGGTCTTCTCGACACGGGCGATGTTCTTGTCCAGGCCCATGCCGTTGAAATAGGGAAACTCggtaggg                                                     >  7:20734/1‑118 (MQ=255)
                                                                                                                     gATGAACGCGGCATCGACGGTGTTCAGGGTGAACACCACGGTCAATGGGTCGGTCTTCTCGACACGGGCGATGTTCTTGTCCAGGCCCATGCCGTTGAAATAGGGAAACTCGGTAGGGTAGGCCTGACGGAACCGATGACCCTTGTc                        >  1:269115/1‑147 (MQ=255)
                                                                                                                       tGAACGCGGCATCGACGGTGTTCAGGGTGAACACCACGGTCAATGGGTCGGTCTTCTCGACACGGGCGATGTTCTTGTCCAGGCCCATGCCg                                                                             <  4:131799/92‑1 (MQ=255)
                                                                                                                       tGAACGCGGCATCGACGGTGTTCAGGGTGAACACCACGGTCAATGGGTCGGTCTTCTCGACACGGGCGATGTTCTTGTCCAGGCCCATGCCg                                                                             >  3:131799/1‑92 (MQ=255)
                                                                                                                           cgcgGCATCGACGGTGTTCAGGGTGAACACCACGGTCAATGGGTCGGTCTTCTCGACACGGGCGATGTTCTTGTCCAGGCCCATGCCGTTGAAATAGGGAAACTCGGTAGGGTAGGCCTGACGGAACCGATGACCCTTGTCGAGCATGc                <  6:171830/149‑1 (MQ=255)
                                                                                                                                cATCGACGGTGTTCAGGGTGAACACCACGGTCAATGGGTCGGTCTTCTCGACACGGGCGATGTTCTTGTCCAGGCCCATGCCGTTGAAATAGGGAAACTCGGTAGGGTAGGCCTGACGGAACCGATGACCCTTGTCGAGCATGCGGtt            >  3:41731/1‑148 (MQ=255)
                                                                                                                                  tCGACGGTGTTCAGGGTGAACACCACGGTCAATGGGTCGGTCTTCTCGACACGGGCGATGTTCTTGTCCAGGCCCATGCCGTTGAAATAGGGAAACTCGGTAGGGTAGGCCTGACGGAACCGATGACCCTTGTCGAGCATGCGGTTGaa         <  5:292146/149‑1 (MQ=255)
                                                                                                                                    cgtggTGTTCACCCTGAACACCGCGGTCAATGGGTCGGTCTTCTCGACACGGGCGATGTTCTTGTCCAGGCCCATGCCGTTGAAATAGGGAAACTCGGTAGGGTAGGCCTGACGGAACCGATGACCCTTGTCGAGCATGCGGTTGAAgg       >  5:202070/4‑149 (MQ=38)
                                                                                                                                     gtggTGTTCACCCTGAACACCACGGTCAATGGGTCGGTCTTCTCGACACGGGCGATGTTCTTGTCCAGGCCCATGCCGTTGAAATAGGGAAACTCGGTAGGGTAGGCCTGACGGaa                                       >  3:60378/3‑116 (MQ=255)
                                                                                                                                     gtggTGTTCACCCTGAACACCACGGTCAATGGGTCGGTCTTCTCGACACGGGCGATGTTCTTGTCCAGGCCCATGCCGTTGAAATAGGGAAACTCGGTAGGGTAGGCCTGACGGaa                                       <  4:60378/114‑1 (MQ=255)
                                                                                                                                        gtgtTCAGGGTGAACACCACGGTCAATGGGTCGGTCTTCTCGACACGGGCGATGTTCTTGTCCAGGCCCATGCCGTTGAAATAGGGAAACTCGGTAGGGTAGGCCTGACGGAACCGATGACCCTTGTCGAGCATGCGGTTGAAGGTGaa   <  7:296374/149‑1 (MQ=255)
                                                                                                                                         tgtTCAGGGTGAACACCACGGTCAATGGGTCGGTCTTCTCGACACGGGCGATGTTCTTGTCCAGGCCCATGCCGTTGAAATAGGGAAACTCGGTAGGGTAGGCCTGACGGAACCGATGACCCTTGTCGAGCATGCGGTTGAAGGTGAAc  <  6:309459/149‑1 (MQ=255)
                                                                                                                                                 |                                                                                                                                            
CACGAACGGCCCGGTACCGATAGGCTGCTGATTGATGTCGCTGGGGCGTCCACTGGCCAGTAGCTGCTCGGCGTATTCAGCGGAGAGGATGGAGGCGAAGCTCATGGCCAGGTTCTGGATGAACGCGGCATCGACGGTGTTCAGGGTGAACACCACGGTCAATGGGTCGGTCTTCTCGACACGGGCGATGTTCTTGTCCAGGCCCATGCCGTTGAAATAGGGAAACTCGGTAGGGTAGGCCTGACGGAACCGATGACCCTTGTCGAGCATGCGGTTGAAGGTGAAC  >  NC_002947/1026341‑1026626

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑