Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 1,027,996 G→C 7.4% A248A (GCC→GCG)  dppA‑III ← dipeptide ABC transporter substrate‑binding protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029471,027,9960GC 7.4% 160.1 / 3.0 54A248A (GCC→GCG) dppA‑IIIdipeptide ABC transporter substrate‑binding protein
Reads supporting (aligned to +/- strand):  ref base G (21/29);  new base C (2/2);  total (23/31)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.99e-01

TCACGTTGTAGGCGATATAGCCGAGGTTGAAACCGGCCTGCTGCGGCATTTGCAGGTTCTTGTCGGCTTTTAGCGGCTCGATGTCGGCCGGGCGTGGGAACAGG‑GTGACCTGGCACTCGTTCTTCTTGAGCTTCTGCATGCGCACCGAGGCGTCGGTGGTGATGGCAAAGATCAGGTTGTCGATCTTCACGTCCTCGGGCTGCCAGTAGTCCTTGTTGCCCTTGAAGCGGATCTGCGCGTCCTTCTGGTATTTGCTGAACACGAACGGGCCGGTGCCGATC  >  NC_002947/1027848‑1028128
                                                                                                                                                     |                                                                                                                                    
tCACGTTGTAGGCGATATAGCCGAGGTTGAAACCGGCCTGCTGCGGCATTTGCAGGTTCTTGTCGGCTTTTAGCGGCTCGATGTCGGCCGGGCGTGGGAACAGG‑GTGACCTGGCACTCGTTCTTCTTGAGCTTCTGCATGCGCACCGAg                                                                                                                                      >  3:299998/1‑149 (MQ=255)
  aCGTTGTAGGCGTTATAGCCGAGGTTGAAACCGGCCTGCTGCGGCATTTGCAGGTTCTTGTCGGCTTTTAGCGGCTCGATGTCGGCCGGGCGTGGGAACAGG‑GTGACCTGGCACTCGTTCTTCTTGAGCTTCTGCATGCGCACCGAGGc                                                                                                                                    <  6:49572/149‑1 (MQ=255)
     ttGTAGGCGATATAGCCGAGGTTGAAACCGGCCTGCTGCGGCATTTGCAGGTTCTTGTCGGCTTTTAGCGGCTCGATGTCGGCCGGGCGTGGGAACAGG‑GTGACCTGGCACTCGTTCTTCTTGAGCTTCTGCATGCGCACCGAGGCGTc                                                                                                                                 >  1:245075/1‑149 (MQ=255)
         aGGCGATATAGCCGAGGTTGAAACCGGCCTGCTGCGGCATTTGCAGGTTCTTGTCGGCTTTTAGCGGCTCGATGTCGGCCGGGCGTGGGAACAGG‑GTGACCTGGCACTCGTTCTTCTTGAGCTTCTGCATGCGCACCGAGGCGTCggtg                                                                                                                             >  3:318790/1‑149 (MQ=255)
          ggCGATATAGCCGAGGTTGAAACCGGCCTGCTGCGGCATTTGCAGGTTCTTGTCGGCTTTTAGCGGCTCGATGTCGGCCGGGCGTGGGAACAGG‑GTGACCTGGCACTCGTTCTTCTTGAGCTTCTGCATGCGCACCGACGCCTCggtgc                                                                                                                            >  6:123471/1‑148 (MQ=255)
             gATATAGCCGAGGTTGAAACCGGCCTGCTGCGGCATTTGCAGGTTCTTGTCGGCTTTTAGCGGCTCGATGTCGGCCGGGCGTGGGAACAGG‑GTGACCTGGCACTCGTTCTTCTTGAGCTTCTGCATGCGCACCGAGGCGTCGGTGGTGa                                                                                                                         <  8:123928/149‑1 (MQ=255)
             gATATAGCCGAGGTTGAAACCGGCCTGCTGCGGCATTTGCAGGTTCTTGTCGGCTTTTAGCGGCTCGATGTCGGCCGGGCGTGGGAACAGG‑GTGACCTGGCACTCGTTCTTCTTGAGCTTCTGCATGCGCACCGAGGCGTCGGTGGTGa                                                                                                                         <  4:313208/149‑1 (MQ=255)
              atatAGCCGAGGTTGAAACCGGCCTGCTGCGGCATTTGCAGGTTCTTGTCGGCTTTTAGCGGCTCGATGTCGGCCGGGCGTGGGAACAGG‑GTGACCTGGCACTCGTTCTTCTTGAGCTTCTGCATGCGCACCGACGCCTCggtgcgcat                                                                                                                        <  5:123471/149‑6 (MQ=255)
               tataGCCGAGGTTGAAACCGGCCTGCTGCGGCATTTGCAGGTTCTTGTCGGCTTTTAGCGGCTCGATGTCGGCCGGGCGTGGGAACAGG‑GTGACCTGGCACTCGTTCTTCTTGAGCTTCTGCATGCGCACCGAGGCGTCGGTGGTGATg                                                                                                                       <  8:79601/149‑1 (MQ=255)
               tataGCCGAGGTTGAAACCGGCCTGCTGCGGCATTTGCAGGTTCTTGTCGGCTTTTAGCGGCTCGATGTCGGCCGGGCGTGGGAACAGG‑GTGAACTGGCACTCGTTCTTCTTGAGCTTCTGCATGCGCACCGAGGCGTCGGTGGTGATg                                                                                                                       >  5:348818/1‑149 (MQ=255)
                    ccGAGGTTGAAACCGGCCTGCTGCGGCATTTGCAGGTTCTTGTCGGCTTTTAGCGGCTCGATGTCGGCCGGGCGTGGGAACAGG‑GTGACCTGGCACTCGTTCTTCTTGAGCTTCTGCATGCGCACCGAGGCGTCGGTGGTGATGGCaaa                                                                                                                  <  6:155153/149‑1 (MQ=255)
                     cGAGGTTGAAACCGGCCTGCTGCGGCATTTGCAGGTTCTTGTCGGCTTTTAGCGGCTCGATGTCGGCCGGGCGTGGGAACAGG‑GTGACCTGGCACTCGTTCTTCTTGAGCTTCTGCATGCGCACCGACGCCTCggtgcgca                                                                                                                         >  4:130809/1‑137 (MQ=255)
                     cGAGGTTGAAACCGGCCTGCTGCGGCATTTGCAGGTTCTTGTCGGCTTTTAGCGGCTCGATGTCGGCCGGGCGTGGGAACAGG‑GTGACCTGGCACTCGTTCTTCTTGAGCTTCTGCATGCGCACCGACGCCTCggtgcgca                                                                                                                         <  3:130809/141‑5 (MQ=255)
                            gAAACCGGCCTGCTGCGGCATTTGCAGGTTCTTGTCGGCTTTTAGCGGCTCGATGTCGGCCGGGCGTGGGAACAGG‑GTGACCTGGCACTCGTTCTTCTTGAGCTTCTGCATGCGCACCGAGGCGTCGGTGGTGATGGCAAAGATCAgg                                                                                                           <  5:106507/148‑1 (MQ=255)
                            gAAACCGGCCTGCTGCGGCATTTGCAGGTTCTTGTCGGCTTTTAGCGGCTCGATGTCGGCCGGGCGTGGGAACAGG‑GTGACCTGGCACTCGTTCTTCTTGAGCTTCTGCATGCGCACCGAGGCGTCGGTGGTGATGGCAAAGATCAgg                                                                                                           <  5:214760/148‑1 (MQ=255)
                                ccGGCCTGCTGCGGCATTTGCAGGTTCTTGTCGGCTTTTAGCGGCTCGATGTCGGCCGGGCGTGGGAACAGG‑GTGACCTGGCACTCGTTCTTCTTGAGCTTCTGCATGCGCACCGAGGCGTCGGTGGTGATGGCAAAGATCAGGTTGTc                                                                                                      <  7:118232/149‑1 (MQ=255)
                                 cGGCCTGCTGCGGCATTTGCAGGTTCTTGTCGGCTTTTAGCGGCTCGATGTCGGCCGGGCGTGGGAACAGG‑GTGACCTGGCACTCGTTCTTCTTGAGCTTCTGCATGCGCACCGAGGCGTCGGTGGTGATGGCAAAGATCAGGTTGTCg                                                                                                     <  7:109657/149‑1 (MQ=255)
                                    ccTGCTGCGGCATTTGCAGGTTCTTGTCGGCTTTTAGCGGCTCGATGTCGGCCGGGCGTGGGAACAGG‑GTGACCTGGCACTCGTTCTTCTTGAGCTTCTGCATGCGCACCGAGGCGTCGGTGGTGATGGCAAAGATCAGGTTGTCGATc                                                                                                  <  4:299998/149‑1 (MQ=255)
                                        ctgcGGCATTTGCAGGTTCTTGTCGGCTTTTAGCGGCTCGATGTCGGCCGGGCGTGGGAACAGG‑GTGACCTGGCACTCGTTCTTCTTGAGCTTCTGCATGCGCACCGAGGCGTCGGTGGTGATGGCAAAGATCAGGTTGTCGATCTTc                                                                                               <  2:245075/148‑1 (MQ=255)
                                           cGGCATTTGCAGGTTCTTGTCGGCTTTTAGCGGCTCGATGTCGGCCGGGCGTGGGAACAGG‑GTGACCTGGCACTCGTTCTTCTTGAGCTTCTGCATGCGCACCGAGGCGTCGGTGGTGATGGCAAAGATCAGGTTGTCGATCTTCACGt                                                                                           >  4:1818/1‑149 (MQ=255)
                                            ggCATTTGCAGGTTCTTGTCGGCTTTTAGCGGCTCGATGTCGGCCGGGCGTGGGAACAGGTGAGACCTGGCACTCGTTATTATTGAGCTTATGCATTCGCACCGAGGCGTCGGTGGTGATGGCAAAGATAAGGTTGTCGATATTCACGt                                                                                           >  1:100489/1‑149 (MQ=255)
                                             gCATTTGCAGGTTCTTGTCGGCTTTTAGCGGCTCGATGTCGGCCGGGCGTGGGAACAGG‑GTGACCTGGCACTCGTTCTTCTTGAGCTTCTGCATGCGCACCGAGGCGTCGGTGGTGATGGCaaa                                                                                                                  >  8:39548/1‑124 (MQ=255)
                                             gCATTTGCAGGTTCTTGTCGGCTTTTAGCGGCTCGATGTCGGCCGGGCGTGGGAACAGG‑GTGACCTGGCACTCGTTCTTCTTGAGCTTCTGCATGCGCACCGAGGCGTCGGTGGTGATGGCAAAGATCAGGTTGTCGATCTTCACGTcc                                                                                         >  2:83316/1‑149 (MQ=255)
                                                  tGCAGGTTCTTGTCGGCTTTTAGCGGCTCGATGTCGGCCGGGCGTGGGAACAGG‑GTGACCTGGCACTCGTTCTTCTTGAGCTTCTGCATGCGCACCGAGGCGTCGGTGGTGATGGCAAAGATCAGGTTGTCGATCTTCACGTCCTCgg                                                                                     <  4:318790/148‑1 (MQ=255)
                                                  tGCAGGTTCTTGTCGGCTTTTAGCGGCTCGATGTCGGCCGGGCGTGGGAACAGG‑GTGACCTGGCACTCGTTCTTCTTGAGCTTCTGCATGCGCACCGAGGCGTCGGTGGTGATGGCAAAGATCAGGTTGTCGATCTTCACGTCCTCgg                                                                                     <  1:83316/148‑1 (MQ=255)
                                                   gCAGGTTCTTGTCGGCTTTTAGCGGCTCGATGTCGGCCGGGCGTGGGAACAGG‑GTGACCTGGCACTCGTTCTTCTTGAGCTTCTGCATGCGCACCGAGGCGTCGGTGGTGATGGCAAAGATCAGGTTGTCGATCTTCACGTCCTCGGGc                                                                                   >  1:233900/1‑149 (MQ=255)
                                                    cAGGTTCTTGTCGGCTTTTAGCGGCTCGATGTCGGCCGGGCGTGGGAACAGG‑GTGACCTGGCACTCGTTCTTCTTGAGCTTCTGCATGCGCACCGAGGCGTCGGTGGTGATGGCAAAGATCAGGTTGTCGATCTTCACGTCCTCGGGc                                                                                   <  2:233900/148‑1 (MQ=255)
                                                           ttGTCGGCTTTTAGCGGCTCGATGTCGGCCGGGCGTGGGAACAGG‑GTGACCTGGCACTCGTTCTTCTTGAGCTTCTGCATGCGCACCGAGGCGTCGGTGGTGATGGCAAAGATCAGGTTGTCGATCTTCACGTCCTCGGGCTGCCag                                                                             >  2:190756/1‑147 (MQ=255)
                                                                       aGCGGCTCGATGTCGGCCGGGCGTGGGAACAGG‑GTGACCTGGCACTCGTTCTTCTTGAGCTTCTGCATGCGCACCGAGGCGTCGGTGGTGATGGCAAAGATCAGGTTGTCGAt                                                                                                   >  6:339496/1‑113 (MQ=255)
                                                                        gCGGCTCGATGTCGGCCGGGCGTGGGAACAGG‑GTGACCTGGCACTCGTTCTTCTTGAGCTTCTGCATGCGCACCGAGGCGTCGGTGGTGATGGCAAAGATCAGGTTGTc                                                                                                      >  1:188960/1‑109 (MQ=255)
                                                                        gCGGCTCGATGTCGGCCGGGCGTGGGAACAGG‑GTGACCTGGCACTCGTTCTTCTTGAGCTTCTGCATGCGCACCGAGGCGTCGGTGGTGATGGCAAAGATCAGGTTGTc                                                                                                      <  2:188960/109‑1 (MQ=255)
                                                                        gCGGCTCGATGTCGGCCGGGCGTGGGAACAGG‑GTGACCTGGCACTCGTTCTTCTTGAGCTTCTGCATGCGCACCGAGGCGTCGGTGGTGATGGCAAAGATCAGGTTGTCGATCTTCACGTCCTc                                                                                       >  1:317278/1‑124 (MQ=255)
                                                                        gCGGCTCGATGTCGGCCGGGCGTGGGAACAGG‑GTGACCTGGCACTCGTTCTTCTTGAGCTTCTGCATGCGCACCGAGGCGTCGGTGGTGATGGCAAAGATCAGGTTGTCGATCTTCACGTCCTc                                                                                       <  2:317278/124‑1 (MQ=255)
                                                                           gCTCGATGTCGGCCGGGCGTGGGAACAGG‑GTGACCTGGCACTCGTTCTTCTTGAGCTTCTGCATGCGCACCGAGGCGTCGGTGGTGATGGCAAAGATCAg                                                                                                            <  1:200679/100‑1 (MQ=255)
                                                                           gCTCGATGTCGGCCGGGCGTGGGAACAGG‑GTGACCTGGCACTCGTTCTTCTTGAGCTTCTGCATGCGCACCGAGGCGTCGGTGGTGATGGCAAAGATCAg                                                                                                            >  2:200679/1‑100 (MQ=255)
                                                                            cTCGATGTCGGCCGGGCGTGGGAACAGG‑GTGACCTGGCACTCGTTCTTCTTGAGCTTCTGCATGCGCACCGAGGCGTCGGTGGTGATGGCAAAGATCAGGTTGTCGATCTTCACGTCCTCGGGCTGCCagta                                                                           >  4:273310/1‑132 (MQ=255)
                                                                            cTCGATGTCGGCCGGGCGTGGGAACAGG‑GTGACCTGGCACTCGTTCTTCTTGAGCTTCTGCATGCGCACCGAGGCGTCGGTGGTGATGGCAAAGATCAGGTTGTCGATCTTCACGTCCTCGGGCTGCCagta                                                                           <  3:273310/132‑1 (MQ=255)
                                                                                       ccGGGCGTGGGAACAGG‑GTGACCTGGCACTCGTTCTTCTTGAGCTTCTGCATGCGCACCGAGGCGTCGGTGGTGATGGCAAAGATCAGGTTGTCGATCTTCACGTCCTCGGGCTGCCAGTAGTCCTTGTTGCCCTTGAAGCGGATCTgc                                               <  6:348818/149‑1 (MQ=255)
                                                                                           gCGTGGGAACAGG‑GTGACCTGGCACTCGTTCTTCTTGAGCTTCTGCATGCGCACCGAGGCGTCGGTGGTGATGGCAAAGATCAGGTTGTCGATCTTCACGTCCTCGGGCTGCCAGTAGTCCTTGTTGCCCTTGa                                                          >  1:147376/1‑134 (MQ=255)
                                                                                           gCGTGGGAACAGG‑GTGACCTGGCACTCGTTCTTCTTGAGCTTCTGCATGCGCACCGAGGCGTCGGTGGTGATGGCAAAGATCAGGTTGTCGATCTTCACGTCCTCGGGCTGCCAGTAGTCCTTGTTGCCCTTGa                                                          <  2:147376/134‑1 (MQ=255)
                                                                                             gTGGGAACAGG‑GTGACCTGGCACTCGTTCTTCTTGAGCTTCTGCATGCGCACCGAGGCGTCGGTGGTGATGGCAAAGATCAGGTTGTCGATCTTCACGTCCTCGGGCTGCCAGTAGTCCt                                                                      >  5:149405/1‑120 (MQ=255)
                                                                                             gTGGGAACAGG‑GTGACCTGGCACTCGTTCTTCTTGAGCTTCTGCATGCGCACCGAGGCGTCGGTGGTGATGGCAAAGATCAGGTTGTCGATCTTCACGTCCTCGGGCTGCCAGTAGTCCt                                                                      <  6:149405/120‑1 (MQ=255)
                                                                                               gggAACAGG‑GTGACCTGGCACTCGTTCTTCTTGAGCTTCTGCATGCGCACCGAGGCGTCGGTGGTGATGGCAAAGATCAGGTTGTCTAGCTTCACGTCCTCGTGCTGCCAGTAGTCATTGTTTCCCTTGAAGAGTATCTGCGCTTCCt                                        >  8:141813/1‑148 (MQ=255)
                                                                                                ggAACAGG‑GTGACCTGGCACTCGTTCTTCTTGAGCTTCTGCATGCGCACCGAGGCGTCGGTGGTGATGGCAAAGATCAGGTTGTCGATCTTCACGTCCTCGGGCTGCCAGTAGTCCTTGTTGCCCTTGAAGCGGATCTGCGCGTCCtt                                       <  7:141813/148‑1 (MQ=255)
                                                                                                ggAACAGG‑GTGACCTGGCACTCGTTCTTCTTGAGCTTCTGCATGCGCACCGAGGCGTCGGTGGTGATGGCAAAGATCAGGTTGTCGATCTTCACGTCCTCGGGCTGCCAGTAGTCCTTGTTGCCCTTGAAGCGGATCTGCGCGTCCTTc                                      <  2:100489/149‑1 (MQ=255)
                                                                                                            aCCTGGCACTCGTTCTTCTTGAGCTTCTGCATGCGCACCGAGGCGTCGGTGGTGATGGCAAAGATCAGGTTGTCGATCTTCACGTCCTCGGGCTGCCAGTAGTCCTTGTTGCCCTTGAAGCGGATCTGCGCGTCCTTCTGGTATTTGCt                           <  1:190756/149‑1 (MQ=255)
                                                                                                                       gTTCTTCTTGAGCTTCTGCATGCGCACCGAGGCGTCGGTGGTGATGGCAAAGATCAGGTTGTCGATCTTCACGTCCTCGGGCTGCCAGTAGTCCTTGTTGCCCTTGAAGCGGATCTGCGCGTCCTTCTGGTATTTGCTGAACACGAACg                >  4:171801/1‑149 (MQ=255)
                                                                                                                              ttGAGCTTCTGCATGCGCACCGAGGCGTCGGTGGTGATGGCAAAGATCAGGTTGTCGATCTTCACGTCCTCGGGCTGCCAGTAGTCCTTGTTGCCCTTGAAGCGGATCTGCGCGTCCTTCTGGTATTTGCTGAACACGAACGGGCCGGt         >  4:36251/1‑149 (MQ=255)
                                                                                                                                 aGCTTCTGCATGCGCACCGAGGCGTCGGTGGTGATGGCAAAGATCAGGTTGTCGATCTTCACGTCCTCGGGCTGCCAGTAGTCCTTGTTGCCCTTGAAGCGGATCTGCGCGTCCTTCTGGTATTTGCTGAACACGAACGGGc             <  4:310598/142‑1 (MQ=255)
                                                                                                                                 aGCTTCTGCATGCGCACCGAGGCGTCGGTGGTGATGGCAAAGATCAGGTTGTCGATCTTCACGTCCTCGGGCTGCCAGTAGTCCTTGTTGCCCTTGAAGCGGATCTGCGCGTCCTTCTGGTATTTGCTGAACACGAACGGGc             >  3:310598/1‑142 (MQ=255)
                                                                                                                                      cTGCATGCGCACCGAGGCGTCGGTGGTGATGGCAAAGATCAGGTTGTCGATCTTCACGTCCTCGGGCTGCCAGTAGTCCTTGTTGCCCTTGAAGCGGATCTGCGCGTCCTTCTGGTATTTGCTGAACACGAACGGGCCGGTGCCGAt   <  3:171801/147‑1 (MQ=255)
                                                                                                                                      cTGCATGCGCACCGAGGCGTCGGTGGTGATGGCAAAGATCAGGTTGTCGATCTTCACGTCCTCGGGCTGCCAGTAGTCCTTGTTGCCCTTGAAGCGGATCTGCGCGTCCTTCTGGTATTTGCTGAACACGAACGGGCCGGTGCCGATc  <  3:1818/148‑1 (MQ=255)
                                                                                                                                        gCATGCGCACCGAGGCGTCGGTGGTGATGGCAAAGATCAGGTTGTCGATCTTCACGTCCTCGGGCTGCCAGTAGTCCTCGTTGCCCTTGAAGCGGATCTGCGCGTCCTTCTGGTATTTGCTGAACACGAAc                 <  2:224119/131‑1 (MQ=255)
                                                                                                                                        gCATGCGCACCGAGGCGTCGGTGGTGATGGCAAAAATCAGGTTGTCGATCTTCACGTCCTCGGGCTGCCAGTAGTCCTCGTTGCCCTTGAAGCGGATCTGCGCGTCCTTCTGGTATTTGCTGAACACGAAc                 >  1:224119/1‑131 (MQ=255)
                                                                                                                                          cagcgcACCGAGGCGTCGGTGGTGATGGCAATGATCAGGTTGTCGATCTTCACGTCCTCGGGCTGCGAGTAGTCCTTGTTGcc                                                               <  6:348702/81‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                    
TCACGTTGTAGGCGATATAGCCGAGGTTGAAACCGGCCTGCTGCGGCATTTGCAGGTTCTTGTCGGCTTTTAGCGGCTCGATGTCGGCCGGGCGTGGGAACAGG‑GTGACCTGGCACTCGTTCTTCTTGAGCTTCTGCATGCGCACCGAGGCGTCGGTGGTGATGGCAAAGATCAGGTTGTCGATCTTCACGTCCTCGGGCTGCCAGTAGTCCTTGTTGCCCTTGAAGCGGATCTGCGCGTCCTTCTGGTATTTGCTGAACACGAACGGGCCGGTGCCGATC  >  NC_002947/1027848‑1028128

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑