Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 1,027,999 G→C 7.3% D247E (GAC→GAG)  dppA‑III ← dipeptide ABC transporter substrate‑binding protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029471,027,9990GC 7.3% 166.9 / 2.5 54D247E (GAC→GAG) dppA‑IIIdipeptide ABC transporter substrate‑binding protein
Reads supporting (aligned to +/- strand):  ref base G (21/29);  new base C (2/2);  total (23/31)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.74e-01

TTGTAGGCGATATAGCCGAGGTTGAAACCGGCCTGCTGCGGCATTTGCAGGTTCTTGTCGGCTTTTAGCGGCTCGATGTCGGCCGGGCGTGGGAACAGG‑GTGACCTGGCACTCGTTCTTCTTGAGCTTCTGCATGCGCACCGAGGCGTCGGTGGTGATGGCAAAGATCAGGTTGTCGATCTTCACGTCCTCGGGCTGCCAGTAGTCCTTGTTGCCCTTGAAGCGGATCTGCGCGTCCTTCTGGTATTTGCTGAACACGAACGGGCCGGTGCCGATCGGCTTCTGGTTGATGTCG  >  NC_002947/1027853‑1028146
                                                                                                                                                   |                                                                                                                                                   
ttGTAGGCGATATAGCCGAGGTTGAAACCGGCCTGCTGCGGCATTTGCAGGTTCTTGTCGGCTTTTAGCGGCTCGATGTCGGCCGGGCGTGGGAACAGG‑GTGACCTGGCACTCGTTCTTCTTGAGCTTCTGCATGCGCACCGAGGCGTc                                                                                                                                                   >  1:245075/1‑149 (MQ=255)
    aGGCGATATAGCCGAGGTTGAAACCGGCCTGCTGCGGCATTTGCAGGTTCTTGTCGGCTTTTAGCGGCTCGATGTCGGCCGGGCGTGGGAACAGG‑GTGACCTGGCACTCGTTCTTCTTGAGCTTCTGCATGCGCACCGAGGCGTCggtg                                                                                                                                               >  3:318790/1‑149 (MQ=255)
     ggCGATATAGCCGAGGTTGAAACCGGCCTGCTGCGGCATTTGCAGGTTCTTGTCGGCTTTTAGCGGCTCGATGTCGGCCGGGCGTGGGAACAGG‑GTGACCTGGCACTCGTTCTTCTTGAGCTTCTGCATGCGCACCGACGCCTCggtgc                                                                                                                                              >  6:123471/1‑148 (MQ=255)
        gATATAGCCGAGGTTGAAACCGGCCTGCTGCGGCATTTGCAGGTTCTTGTCGGCTTTTAGCGGCTCGATGTCGGCCGGGCGTGGGAACAGG‑GTGACCTGGCACTCGTTCTTCTTGAGCTTCTGCATGCGCACCGAGGCGTCGGTGGTGa                                                                                                                                           <  8:123928/149‑1 (MQ=255)
        gATATAGCCGAGGTTGAAACCGGCCTGCTGCGGCATTTGCAGGTTCTTGTCGGCTTTTAGCGGCTCGATGTCGGCCGGGCGTGGGAACAGG‑GTGACCTGGCACTCGTTCTTCTTGAGCTTCTGCATGCGCACCGAGGCGTCGGTGGTGa                                                                                                                                           <  4:313208/149‑1 (MQ=255)
         atatAGCCGAGGTTGAAACCGGCCTGCTGCGGCATTTGCAGGTTCTTGTCGGCTTTTAGCGGCTCGATGTCGGCCGGGCGTGGGAACAGG‑GTGACCTGGCACTCGTTCTTCTTGAGCTTCTGCATGCGCACCGACGCCTCggtgcgcat                                                                                                                                          <  5:123471/149‑6 (MQ=255)
          tataGCCGAGGTTGAAACCGGCCTGCTGCGGCATTTGCAGGTTCTTGTCGGCTTTTAGCGGCTCGATGTCGGCCGGGCGTGGGAACAGG‑GTGACCTGGCACTCGTTCTTCTTGAGCTTCTGCATGCGCACCGAGGCGTCGGTGGTGATg                                                                                                                                         <  8:79601/149‑1 (MQ=255)
          tataGCCGAGGTTGAAACCGGCCTGCTGCGGCATTTGCAGGTTCTTGTCGGCTTTTAGCGGCTCGATGTCGGCCGGGCGTGGGAACAGG‑GTGAACTGGCACTCGTTCTTCTTGAGCTTCTGCATGCGCACCGAGGCGTCGGTGGTGATg                                                                                                                                         >  5:348818/1‑149 (MQ=255)
               ccGAGGTTGAAACCGGCCTGCTGCGGCATTTGCAGGTTCTTGTCGGCTTTTAGCGGCTCGATGTCGGCCGGGCGTGGGAACAGG‑GTGACCTGGCACTCGTTCTTCTTGAGCTTCTGCATGCGCACCGAGGCGTCGGTGGTGATGGCaaa                                                                                                                                    <  6:155153/149‑1 (MQ=255)
                cGAGGTTGAAACCGGCCTGCTGCGGCATTTGCAGGTTCTTGTCGGCTTTTAGCGGCTCGATGTCGGCCGGGCGTGGGAACAGG‑GTGACCTGGCACTCGTTCTTCTTGAGCTTCTGCATGCGCACCGACGCCTCggtgcgca                                                                                                                                           >  4:130809/1‑137 (MQ=255)
                cGAGGTTGAAACCGGCCTGCTGCGGCATTTGCAGGTTCTTGTCGGCTTTTAGCGGCTCGATGTCGGCCGGGCGTGGGAACAGG‑GTGACCTGGCACTCGTTCTTCTTGAGCTTCTGCATGCGCACCGACGCCTCggtgcgca                                                                                                                                           <  3:130809/141‑5 (MQ=255)
                       gAAACCGGCCTGCTGCGGCATTTGCAGGTTCTTGTCGGCTTTTAGCGGCTCGATGTCGGCCGGGCGTGGGAACAGG‑GTGACCTGGCACTCGTTCTTCTTGAGCTTCTGCATGCGCACCGAGGCGTCGGTGGTGATGGCAAAGATCAgg                                                                                                                             <  5:106507/148‑1 (MQ=255)
                       gAAACCGGCCTGCTGCGGCATTTGCAGGTTCTTGTCGGCTTTTAGCGGCTCGATGTCGGCCGGGCGTGGGAACAGG‑GTGACCTGGCACTCGTTCTTCTTGAGCTTCTGCATGCGCACCGAGGCGTCGGTGGTGATGGCAAAGATCAgg                                                                                                                             <  5:214760/148‑1 (MQ=255)
                           ccGGCCTGCTGCGGCATTTGCAGGTTCTTGTCGGCTTTTAGCGGCTCGATGTCGGCCGGGCGTGGGAACAGG‑GTGACCTGGCACTCGTTCTTCTTGAGCTTCTGCATGCGCACCGAGGCGTCGGTGGTGATGGCAAAGATCAGGTTGTc                                                                                                                        <  7:118232/149‑1 (MQ=255)
                            cGGCCTGCTGCGGCATTTGCAGGTTCTTGTCGGCTTTTAGCGGCTCGATGTCGGCCGGGCGTGGGAACAGG‑GTGACCTGGCACTCGTTCTTCTTGAGCTTCTGCATGCGCACCGAGGCGTCGGTGGTGATGGCAAAGATCAGGTTGTCg                                                                                                                       <  7:109657/149‑1 (MQ=255)
                               ccTGCTGCGGCATTTGCAGGTTCTTGTCGGCTTTTAGCGGCTCGATGTCGGCCGGGCGTGGGAACAGG‑GTGACCTGGCACTCGTTCTTCTTGAGCTTCTGCATGCGCACCGAGGCGTCGGTGGTGATGGCAAAGATCAGGTTGTCGATc                                                                                                                    <  4:299998/149‑1 (MQ=255)
                                   ctgcGGCATTTGCAGGTTCTTGTCGGCTTTTAGCGGCTCGATGTCGGCCGGGCGTGGGAACAGG‑GTGACCTGGCACTCGTTCTTCTTGAGCTTCTGCATGCGCACCGAGGCGTCGGTGGTGATGGCAAAGATCAGGTTGTCGATCTTc                                                                                                                 <  2:245075/148‑1 (MQ=255)
                                      cGGCATTTGCAGGTTCTTGTCGGCTTTTAGCGGCTCGATGTCGGCCGGGCGTGGGAACAGG‑GTGACCTGGCACTCGTTCTTCTTGAGCTTCTGCATGCGCACCGAGGCGTCGGTGGTGATGGCAAAGATCAGGTTGTCGATCTTCACGt                                                                                                             >  4:1818/1‑149 (MQ=255)
                                       ggCATTTGCAGGTTCTTGTCGGCTTTTAGCGGCTCGATGTCGGCCGGGCGTGGGAACAGGTGAGACCTGGCACTCGTTATTATTGAGCTTATGCATTCGCACCGAGGCGTCGGTGGTGATGGCAAAGATAAGGTTGTCGATATTCACGt                                                                                                             >  1:100489/1‑149 (MQ=255)
                                        gCATTTGCAGGTTCTTGTCGGCTTTTAGCGGCTCGATGTCGGCCGGGCGTGGGAACAGG‑GTGACCTGGCACTCGTTCTTCTTGAGCTTCTGCATGCGCACCGAGGCGTCGGTGGTGATGGCaaa                                                                                                                                    >  8:39548/1‑124 (MQ=255)
                                        gCATTTGCAGGTTCTTGTCGGCTTTTAGCGGCTCGATGTCGGCCGGGCGTGGGAACAGG‑GTGACCTGGCACTCGTTCTTCTTGAGCTTCTGCATGCGCACCGAGGCGTCGGTGGTGATGGCAAAGATCAGGTTGTCGATCTTCACGTcc                                                                                                           >  2:83316/1‑149 (MQ=255)
                                             tGCAGGTTCTTGTCGGCTTTTAGCGGCTCGATGTCGGCCGGGCGTGGGAACAGG‑GTGACCTGGCACTCGTTCTTCTTGAGCTTCTGCATGCGCACCGAGGCGTCGGTGGTGATGGCAAAGATCAGGTTGTCGATCTTCACGTCCTCgg                                                                                                       <  4:318790/148‑1 (MQ=255)
                                             tGCAGGTTCTTGTCGGCTTTTAGCGGCTCGATGTCGGCCGGGCGTGGGAACAGG‑GTGACCTGGCACTCGTTCTTCTTGAGCTTCTGCATGCGCACCGAGGCGTCGGTGGTGATGGCAAAGATCAGGTTGTCGATCTTCACGTCCTCgg                                                                                                       <  1:83316/148‑1 (MQ=255)
                                              gCAGGTTCTTGTCGGCTTTTAGCGGCTCGATGTCGGCCGGGCGTGGGAACAGG‑GTGACCTGGCACTCGTTCTTCTTGAGCTTCTGCATGCGCACCGAGGCGTCGGTGGTGATGGCAAAGATCAGGTTGTCGATCTTCACGTCCTCGGGc                                                                                                     >  1:233900/1‑149 (MQ=255)
                                               cAGGTTCTTGTCGGCTTTTAGCGGCTCGATGTCGGCCGGGCGTGGGAACAGG‑GTGACCTGGCACTCGTTCTTCTTGAGCTTCTGCATGCGCACCGAGGCGTCGGTGGTGATGGCAAAGATCAGGTTGTCGATCTTCACGTCCTCGGGc                                                                                                     <  2:233900/148‑1 (MQ=255)
                                                      ttGTCGGCTTTTAGCGGCTCGATGTCGGCCGGGCGTGGGAACAGG‑GTGACCTGGCACTCGTTCTTCTTGAGCTTCTGCATGCGCACCGAGGCGTCGGTGGTGATGGCAAAGATCAGGTTGTCGATCTTCACGTCCTCGGGCTGCCag                                                                                               >  2:190756/1‑147 (MQ=255)
                                                                  aGCGGCTCGATGTCGGCCGGGCGTGGGAACAGG‑GTGACCTGGCACTCGTTCTTCTTGAGCTTCTGCATGCGCACCGAGGCGTCGGTGGTGATGGCAAAGATCAGGTTGTCGAt                                                                                                                     >  6:339496/1‑113 (MQ=255)
                                                                   gCGGCTCGATGTCGGCCGGGCGTGGGAACAGG‑GTGACCTGGCACTCGTTCTTCTTGAGCTTCTGCATGCGCACCGAGGCGTCGGTGGTGATGGCAAAGATCAGGTTGTc                                                                                                                        <  2:188960/109‑1 (MQ=255)
                                                                   gCGGCTCGATGTCGGCCGGGCGTGGGAACAGG‑GTGACCTGGCACTCGTTCTTCTTGAGCTTCTGCATGCGCACCGAGGCGTCGGTGGTGATGGCAAAGATCAGGTTGTc                                                                                                                        >  1:188960/1‑109 (MQ=255)
                                                                   gCGGCTCGATGTCGGCCGGGCGTGGGAACAGG‑GTGACCTGGCACTCGTTCTTCTTGAGCTTCTGCATGCGCACCGAGGCGTCGGTGGTGATGGCAAAGATCAGGTTGTCGATCTTCACGTCCTc                                                                                                         >  1:317278/1‑124 (MQ=255)
                                                                   gCGGCTCGATGTCGGCCGGGCGTGGGAACAGG‑GTGACCTGGCACTCGTTCTTCTTGAGCTTCTGCATGCGCACCGAGGCGTCGGTGGTGATGGCAAAGATCAGGTTGTCGATCTTCACGTCCTc                                                                                                         <  2:317278/124‑1 (MQ=255)
                                                                      gCTCGATGTCGGCCGGGCGTGGGAACAGG‑GTGACCTGGCACTCGTTCTTCTTGAGCTTCTGCATGCGCACCGAGGCGTCGGTGGTGATGGCAAAGATCAg                                                                                                                              <  1:200679/100‑1 (MQ=255)
                                                                      gCTCGATGTCGGCCGGGCGTGGGAACAGG‑GTGACCTGGCACTCGTTCTTCTTGAGCTTCTGCATGCGCACCGAGGCGTCGGTGGTGATGGCAAAGATCAg                                                                                                                              >  2:200679/1‑100 (MQ=255)
                                                                       cTCGATGTCGGCCGGGCGTGGGAACAGG‑GTGACCTGGCACTCGTTCTTCTTGAGCTTCTGCATGCGCACCGAGGCGTCGGTGGTGATGGCAAAGATCAGGTTGTCGATCTTCACGTCCTCGGGCTGCCagta                                                                                             <  3:273310/132‑1 (MQ=255)
                                                                       cTCGATGTCGGCCGGGCGTGGGAACAGG‑GTGACCTGGCACTCGTTCTTCTTGAGCTTCTGCATGCGCACCGAGGCGTCGGTGGTGATGGCAAAGATCAGGTTGTCGATCTTCACGTCCTCGGGCTGCCagta                                                                                             >  4:273310/1‑132 (MQ=255)
                                                                                  ccGGGCGTGGGAACAGG‑GTGACCTGGCACTCGTTCTTCTTGAGCTTCTGCATGCGCACCGAGGCGTCGGTGGTGATGGCAAAGATCAGGTTGTCGATCTTCACGTCCTCGGGCTGCCAGTAGTCCTTGTTGCCCTTGAAGCGGATCTgc                                                                 <  6:348818/149‑1 (MQ=255)
                                                                                      gCGTGGGAACAGG‑GTGACCTGGCACTCGTTCTTCTTGAGCTTCTGCATGCGCACCGAGGCGTCGGTGGTGATGGCAAAGATCAGGTTGTCGATCTTCACGTCCTCGGGCTGCCAGTAGTCCTTGTTGCCCTTGa                                                                            <  2:147376/134‑1 (MQ=255)
                                                                                      gCGTGGGAACAGG‑GTGACCTGGCACTCGTTCTTCTTGAGCTTCTGCATGCGCACCGAGGCGTCGGTGGTGATGGCAAAGATCAGGTTGTCGATCTTCACGTCCTCGGGCTGCCAGTAGTCCTTGTTGCCCTTGa                                                                            >  1:147376/1‑134 (MQ=255)
                                                                                        gTGGGAACAGG‑GTGACCTGGCACTCGTTCTTCTTGAGCTTCTGCATGCGCACCGAGGCGTCGGTGGTGATGGCAAAGATCAGGTTGTCGATCTTCACGTCCTCGGGCTGCCAGTAGTCCt                                                                                        >  5:149405/1‑120 (MQ=255)
                                                                                        gTGGGAACAGG‑GTGACCTGGCACTCGTTCTTCTTGAGCTTCTGCATGCGCACCGAGGCGTCGGTGGTGATGGCAAAGATCAGGTTGTCGATCTTCACGTCCTCGGGCTGCCAGTAGTCCt                                                                                        <  6:149405/120‑1 (MQ=255)
                                                                                          gggAACAGG‑GTGACCTGGCACTCGTTCTTCTTGAGCTTCTGCATGCGCACCGAGGCGTCGGTGGTGATGGCAAAGATCAGGTTGTCTAGCTTCACGTCCTCGTGCTGCCAGTAGTCATTGTTTCCCTTGAAGAGTATCTGCGCTTCCt                                                          >  8:141813/1‑148 (MQ=255)
                                                                                           ggAACAGG‑GTGACCTGGCACTCGTTCTTCTTGAGCTTCTGCATGCGCACCGAGGCGTCGGTGGTGATGGCAAAGATCAGGTTGTCGATCTTCACGTCCTCGGGCTGCCAGTAGTCCTTGTTGCCCTTGAAGCGGATCTGCGCGTCCtt                                                         <  7:141813/148‑1 (MQ=255)
                                                                                           ggAACAGG‑GTGACCTGGCACTCGTTCTTCTTGAGCTTCTGCATGCGCACCGAGGCGTCGGTGGTGATGGCAAAGATCAGGTTGTCGATCTTCACGTCCTCGGGCTGCCAGTAGTCCTTGTTGCCCTTGAAGCGGATCTGCGCGTCCTTc                                                        <  2:100489/149‑1 (MQ=255)
                                                                                                       aCCTGGCACTCGTTCTTCTTGAGCTTCTGCATGCGCACCGAGGCGTCGGTGGTGATGGCAAAGATCAGGTTGTCGATCTTCACGTCCTCGGGCTGCCAGTAGTCCTTGTTGCCCTTGAAGCGGATCTGCGCGTCCTTCTGGTATTTGCt                                             <  1:190756/149‑1 (MQ=255)
                                                                                                                  gTTCTTCTTGAGCTTCTGCATGCGCACCGAGGCGTCGGTGGTGATGGCAAAGATCAGGTTGTCGATCTTCACGTCCTCGGGCTGCCAGTAGTCCTTGTTGCCCTTGAAGCGGATCTGCGCGTCCTTCTGGTATTTGCTGAACACGAACg                                  >  4:171801/1‑149 (MQ=255)
                                                                                                                         ttGAGCTTCTGCATGCGCACCGAGGCGTCGGTGGTGATGGCAAAGATCAGGTTGTCGATCTTCACGTCCTCGGGCTGCCAGTAGTCCTTGTTGCCCTTGAAGCGGATCTGCGCGTCCTTCTGGTATTTGCTGAACACGAACGGGCCGGt                           >  4:36251/1‑149 (MQ=255)
                                                                                                                            aGCTTCTGCATGCGCACCGAGGCGTCGGTGGTGATGGCAAAGATCAGGTTGTCGATCTTCACGTCCTCGGGCTGCCAGTAGTCCTTGTTGCCCTTGAAGCGGATCTGCGCGTCCTTCTGGTATTTGCTGAACACGAACGGGc                               <  4:310598/142‑1 (MQ=255)
                                                                                                                            aGCTTCTGCATGCGCACCGAGGCGTCGGTGGTGATGGCAAAGATCAGGTTGTCGATCTTCACGTCCTCGGGCTGCCAGTAGTCCTTGTTGCCCTTGAAGCGGATCTGCGCGTCCTTCTGGTATTTGCTGAACACGAACGGGc                               >  3:310598/1‑142 (MQ=255)
                                                                                                                                 cTGCATGCGCACCGAGGCGTCGGTGGTGATGGCAAAGATCAGGTTGTCGATCTTCACGTCCTCGGGCTGCCAGTAGTCCTTGTTGCCCTTGAAGCGGATCTGCGCGTCCTTCTGGTATTTGCTGAACACGAACGGGCCGGTGCCGAt                     <  3:171801/147‑1 (MQ=255)
                                                                                                                                 cTGCATGCGCACCGAGGCGTCGGTGGTGATGGCAAAGATCAGGTTGTCGATCTTCACGTCCTCGGGCTGCCAGTAGTCCTTGTTGCCCTTGAAGCGGATCTGCGCGTCCTTCTGGTATTTGCTGAACACGAACGGGCCGGTGCCGATc                    <  3:1818/148‑1 (MQ=255)
                                                                                                                                   gCATGCGCACCGAGGCGTCGGTGGTGATGGCAAAGATCAGGTTGTCGATCTTCACGTCCTCGGGCTGCCAGTAGTCCTCGTTGCCCTTGAAGCGGATCTGCGCGTCCTTCTGGTATTTGCTGAACACGAAc                                   <  2:224119/131‑1 (MQ=255)
                                                                                                                                   gCATGCGCACCGAGGCGTCGGTGGTGATGGCAAAAATCAGGTTGTCGATCTTCACGTCCTCGGGCTGCCAGTAGTCCTCGTTGCCCTTGAAGCGGATCTGCGCGTCCTTCTGGTATTTGCTGAACACGAAc                                   >  1:224119/1‑131 (MQ=255)
                                                                                                                                     cagcgcACCGAGGCGTCGGTGGTGATGGCAATGATCAGGTTGTCGATCTTCACGTCCTCGGGCTGCGAGTAGTCCTTGTTGcc                                                                                 <  6:348702/81‑1 (MQ=255)
                                                                                                                                                  cGTCGGTGGTGATGGCAAAGATCAGGTTGTCGATCTTCACGTCCTCGGGCTGCCAGTAGTCCTTGGTGCCCTTGAAGCGGATCTGCGCGTCCTTCTGGTATTTGCTGAACACGAACGGGCCGGTGCCGATCGGCTTCTGGTTGATGTcg  <  3:270005/149‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                   
TTGTAGGCGATATAGCCGAGGTTGAAACCGGCCTGCTGCGGCATTTGCAGGTTCTTGTCGGCTTTTAGCGGCTCGATGTCGGCCGGGCGTGGGAACAGG‑GTGACCTGGCACTCGTTCTTCTTGAGCTTCTGCATGCGCACCGAGGCGTCGGTGGTGATGGCAAAGATCAGGTTGTCGATCTTCACGTCCTCGGGCTGCCAGTAGTCCTTGTTGCCCTTGAAGCGGATCTGCGCGTCCTTCTGGTATTTGCTGAACACGAACGGGCCGGTGCCGATCGGCTTCTGGTTGATGTCG  >  NC_002947/1027853‑1028146

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑