Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 1,073,189 G→T 7.5% A68A (GCC→GCA)  gatB ← aspartyl/glutamyl‑tRNA(Asn/Gln) amidotransferase subunit B

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029471,073,1890GT 7.5% 166.2 / 4.1 53A68A (GCC→GCA) gatBaspartyl/glutamyl‑tRNA(Asn/Gln) amidotransferase subunit B
Reads supporting (aligned to +/- strand):  ref base G (26/23);  new base T (2/2);  total (28/25)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.69e-01

GATGCGCTTGATGGTGCCGTCTTCCAGCGCGATGTCCAGGTGACCCTTGCCGACGATCGGCAGGTCCATCTGGCTGATCTGGTAGCCCTTGGGCAGGTCGGGGTAGAAGTAGTTCTTGCGTGCGAACACGTTGCGCTTGCCGATCTCGGCATCGATGGCCAGGCCGAACATGCACGCCATGCGCACGGCTTGCTCGTTCAGCACCGGCAGTACGCCGGGCATGCCCAGGTCAACCAGGCTGGCCTGAGTATTCGGCTCGGAGCCAAAGGTGGTAGCGCTGCCGGAGAAGATCTT  >  NC_002947/1073042‑1073335
                                                                                                                                                   |                                                                                                                                                  
gATGCGCTTGATGGTGCCGTCTTCCAGCGCGATGTCCAGGTGACCCTTGCCGACGATCGGCAGGTCCATCTGGCTGATCTGGTAGCCCTTGGGCAGGTCGGGGTAGAAGTAGTTCTTGCGTGCGAACACGTTGCGCTTGCCGATCTCgg                                                                                                                                                   <  5:15658/149‑1 (MQ=255)
  tGCGCTTGATGGTGCCGTCTTCCAGCGCGATGTCCAGGTGACCCTTGCCGACGATCGGCAGGTCCATCTGGCTGATCTGGTAGCCCTTGGGCAGGTCGGGGTAGAAGTAGTTCTTGCGTGCGAACACGTTGCGCTTGCCGATCTCGGCa                                                                                                                                                 <  8:173521/149‑1 (MQ=255)
    cgcTTGATGGTGCCGTCTTCCAGCGCGATGTCCAGGTGACCCTTGCCGACGATCGGCAGGTCCATCTGGCTGATCTGGTAGCCCTTGGGCAGGTCGGGGTAGAAGTAGTTCTTGCGTGCGAACACGTTGCGCTTGCCGATCTCGGCATc                                                                                                                                               <  6:350074/149‑1 (MQ=255)
     gcTTGATGGTGCCGTCTTCCAGCGCGATGTCCAGGTGACCCTTGCCGACGATCGGCAGGTCCATCTGGCTGATCTGGTAGCCCTTGGGCAGGTCGGGGTAGAAGTAGTTCTTGCGTGCGAACACGTTGCGCTTGCCGAGATCGGCaagc                                                                                                                                              <  2:106092/149‑4 (MQ=255)
         gATGGTGCCGTCTTCCAGCGCGATGTCCAGGTGACCCTTGCCGACGATCGGCAGGTCCATCTGGCTGATCTGGTAGCCCTTGGGCAGGTCGGGGTAGAAGTAGTTCTTGCGTGCGAACACGTTGCGCTTGCCGATCTCGGCATCGATg                                                                                                                                           <  4:52374/148‑1 (MQ=255)
         gATGGGCCCGTCTTCCACCGCCATGGCCAGGGGACCCTTGCCGACGATCGGCAGGTCCATCTGGCTGATCTGGTAGCCCTTGGGCAGGTCGGGGTAGAAGTAGTTCTTGCGTGCGAACACGTTGCGCTTGCCGATCTCGGCATCGATg                                                                                                                                           <  6:289990/148‑1 (MQ=255)
           tGGTGCCGTCTTCCAGCGCGATGTCCAGGTGACCCTTGCCGACGATCGGCAGGTCCATCTGGCTGATCTGGTAGCCCTTGGGCAGGTCGGGGTAGAAGTAGTTCTTGCGTGCGAACACGTTGCGCTTGCCGATCTCGGCATCGATGGcc                                                                                                                                        <  8:231196/149‑1 (MQ=255)
             gTGCCGTCTTCCAGCGCGATGTCCAGGTGACCCTTGCCGACGATCGGCAGGTCCATCTGGCTGATCTGGTAGCCCTTGGGCAGGTCGGGGTAGAAGTAGTTCTTGCGTGCGAACACGTTGCGCTTGCCGATCTCTGCATCGATGGCCAg                                                                                                                                      >  6:233876/1‑149 (MQ=255)
             gTGCCGTCTTCCAGCGCGATGTCCAGGTGACCCTTGCCGACGATCGGCAGGTCCATCTGGCTGATCTGGTAGCCCTTGGGCAGGTCGGGGTAGAAGTAGTTCTTGCGTGCGAACACGTTGCGCTTGCCGATCTCTGCATCGATGGCCAg                                                                                                                                      >  6:238953/1‑149 (MQ=255)
                     ttCCAGCGCGATGTCCAGGTGACCCTTGCCGACGATCGGCAGGTCCATCTGGCTGATCTGGTAGCCCTTGGGCAGGTCGGGGTAGAAGTAGTTCTTGCGTGCGAACACGTTGCGCTTGCCGATCTCGGCATCGATGGCCAGGCCGAAc                                                                                                                               >  4:322744/1‑148 (MQ=255)
                       ccAGCGCGATGTCCAGGTGACCCTTGCCGACGATCGGCAGGTCCATCTGGCTGATCTGGTAGCCCTTGGGCAGGTCGGGGTAGAAGTAGTTCTTGCGTGCGAACACGTTGCGCTTGCCGATCTCGGCATCGATGGCCAGGCCGAAc                                                                                                                               <  3:322744/146‑1 (MQ=255)
                          gcgcGATGTCCAGGTGACCCTTGCCGACGATCGGCAGGTCCATCTGGCAGATCTGGTAGCCCTTGGGCAGGTCGGGGTAGAAGTAGTTCTTGCGTGCGAACACGTTGCGCTTGCCGATCTCGGCATCGATGGCCAGGCCGAACATGCAc                                                                                                                         >  1:80021/1‑149 (MQ=255)
                              gATGTCCAGGTGACCCTTGCCGACGATCGGCAGGTCCATCTGGCTGATCTGGTAGCCCTTGGGCAGGTCGGGGTAGAAGTAGTTCTTGCGTGCGAACACGTTGCGCTTGCCGATCTCGGCATCGATGGCCAGGCCGAACATGCACGCCa                                                                                                                     >  3:60801/1‑149 (MQ=255)
                                tGTCCAGGTGACCCTTGCCGACGATCGGCAGGTCCATCTGGCTGATCTGGTAGCCCTTGGGCAGGTCGGGGTAGAAGTAGTTCTTGCGTGCGAACACGTTGCGCTTGCCGATCTCTGCATCGATGGCCAGGCCGAACATGCACGCCATg                                                                                                                   <  5:233876/149‑1 (MQ=255)
                                tGTCCAGGTGACCCTTGCCGACGATCGGCAGGTCCATCTGGCTGATCTGGTAGCCCTTGGGCAGGTCGGGGTAGAAGTAGTTCTTGCGTGCGAACACGTTGCGCTTGCCGATCTCTGCATCGATGGCCAGGCCGAACATGCACGCCATg                                                                                                                   <  5:238953/149‑1 (MQ=255)
                                                gCCGACGATCGGCAGGTCCATCTGGCTGATCTGGTAGCCCTTGGGCAGGTCGGGGTAGAAGTAGTTCTTGCGTGCGAACACGTTGCGCTTGCCGATCTCGGCATCGATGGCCAGGCCGAACATGCACGCCATGCGCACGGCt                                                                                                          >  3:332647/1‑142 (MQ=255)
                                                gCCGACGATCGGCAGGTCCATCTGGCTGATCTGGTAGCCCTTGGGCAGGTCGGGGTAGAAGTAGTTCTTGCGTGCGAACACGTTGCGCTTGCCGATCTCGGCATCGATGGCCAGGCCGAACATGCACGCCATGCGCACGGCt                                                                                                          <  4:332647/142‑1 (MQ=255)
                                                  cgacgaTCGGCAGGTCCATCTGGCTGATCTGGTAGCCCTTGGGCAGGTCGGGGTAGAAGTAGTTCTTGCGTGCGAACACGTTGCGCTTGCCGATCTCGGCATCGATGGCCAGGCCGAACATGCACGCCATGCGCACGGCTTGCTCGtt                                                                                                  >  2:161097/1‑148 (MQ=255)
                                                           gCAGGTCCATCTGGCTGATCTGGTAGCCCTTGGGCAGGTCGGGGTAGAAGTAGTTCTTGCGTGCGAACACGTTGCGCTTGCCGATCTCGGCATCGATGGCCAGGCCGAACATGCACGCCATGCGCACGGCTTGCTCGTTCAGCACCgg                                                                                         >  8:273775/1‑148 (MQ=255)
                                                           gCAGGTCCATCTGGCTGATCTGGTAGCCCTTGGGCAGGTCGGGGTAGAAGTAGTTCTTGCGTGCGAACACGTTGCGCTTGCCGATCTCGGCATCGATGGCCAGGCCGAACATGCACGCCATGCGCACGGCTTGCTCGTTCAGCACCGGc                                                                                        <  2:80021/149‑1 (MQ=255)
                                                               gTCCATCTGGCTGATCTGGTAGCCCTTGGGCAGGTCGGGGTAGAAGTAGTTCTTGCGTGCGAACACGTTGCGCTTGCCGATCTCGGCATCGATGGCCAGGCCGAACATGCACGCCATGCGCACGGCTTGCTCGTTCAGCACCGGCAGTa                                                                                    >  2:242328/1‑149 (MQ=255)
                                                                 ccATCTGGCTGATCTGGTAGCCCTTGGGCAGGTCGGGGTAGAAGTAGTTCTTGCGTGCGAACACGTTGCGCTTGCCGATCTCGGCATCGATGGCCAGGCCGAACATGCACGCCATGCGCACGGCTTGCTCGTTCAGCACCGGCAGTACg                                                                                  >  2:291268/1‑149 (MQ=255)
                                                                  cATCTTGCTGATCTCGTGGCCCTTGCGCAGGTCGGGGTAGAAGTAGTTCTTGCGTGCTAACACCTTGCTCTTGCCGATCTCGGCATAGGTCGCCAGGCCCAACATCCACGCCATGCGCACGCCTTGCTCGTTCAGCACCGGCAGTAAGc                                                                                 >  8:269644/1‑149 (MQ=255)
                                                                  cATCTGGCTGATCTGGTAGCCCTTGGGCAGGTCGGGGTAGAAGTAGTTCTTGCGTGCGAACACGTTGCGCTTGCCGATCTCGGCATCGATGGCCAGGCCGAACATGCACGCCATGCGCACGGCTTGCTCGTTCAGCACCGGCAGTACGc                                                                                 <  1:291268/149‑1 (MQ=255)
                                                                          tGATCTGGTAGCCCTTGGGCAGGTCGGGGTAGAAGTAGTTCTTGCGTGCGAACACGTTGCGCTTGCCGATCTCGGCATCGATgg                                                                                                                                          >  8:198475/1‑84 (MQ=255)
                                                                          tGATCTGGTAGCCCTTGGGCAGGTCGGGGTAGAAGTAGTTCTTGCGTGCGAACACGTTGCGCTTGCCGATCTCGGCATCGATGGCCAGGCCGAACATGCACGCCATGCGCACGGCTTGCTCGTTCAGCACCGGCAGTACGCCGGGCATg                                                                         >  4:32526/1‑149 (MQ=255)
                                                                               tGGTAGCCCTTGGGCAGGTCGGGGTAGAAGTAGTTCTTGCGTGCGAACACGTTGCGCTTGCCGATCTCGGCATCGATGGCCAGGCCGAACATGAACGCCATGCGCACGGCTTGCTCGTTCAGCACCGGCAGTACGACGTGCATGCCCAg                                                                    >  5:190490/1‑149 (MQ=255)
                                                                                 gTAGCCCTTGGGCAGGTCGGGGTAGAAGTAGTTCTTGCGTGCGAACACGTTGCGCTTGCCGATCTCGGCATCGATGGCCAGGCCGAACATGCACGCCATGCGCACGGCTTGCTCGTTCAGCACCGGCAGTACGCCGGGCATGCCCAg                                                                    <  4:60801/147‑1 (MQ=255)
                                                                                 gTAGCCCTTGGGCAGGTCGGGGTAGAAGTAGTTCTTGCGTGCGAACACGTTGCGCTTGCCGATCTCGGCATCGATGGCCAGGCCGAACATGCACGCCATGCGCACGGCTTGCTCGTTCAGCACCGGCAGTACGCCGGGCATGCCCAg                                                                    <  1:161097/147‑1 (MQ=255)
                                                                                    gCCCTTGGGCAGGTCGGGGTAGAAGTAGTTCTTGCGTGCGAACACGTTGCGCTTGCCGATCTCGGCATCGATGGCCAGGCCGAACATGCACGCCATGCGCACGGCTTGCTCGTTCAGCACCGGCAGTACGCCGGGCATGCCCAGGTCa                                                                <  1:242328/148‑1 (MQ=255)
                                                                                           ggCAGGTCGGGGTAGAAGTAGTTCTTGCGTGCGAACACGTTGCGCTTGCCGATCTCGGCATCGATGGCCAGGCCGAACATGCACGCCATGCGCACGGCTTGCTCGTTCAGCa                                                                                             >  5:251341/1‑112 (MQ=255)
                                                                                           ggCAGGTCGGGGTAGAAGTAGTTCTTGCGTGCGAACACGTTGCGCTTGCCGATCTCGGCATCGATGGCCAGGCCGAACATGCACGCCATGCGCACGGCTTGCTCGTTCAGCa                                                                                             <  6:251341/112‑1 (MQ=255)
                                                                                            gCAGGTCGGGGTAGAAGTAGTTCTTGAGTGCGAACACGTTGCGCTTGCCGATCTCGGCATCGATGGCCAGGCCGAACATGCACGCCATGCGCACGGCTTCCTCGTTCAGCACCGCCAGTACGCCGGGAATGCCCAGGTCAACCAGGCTg                                                       >  2:120063/1‑149 (MQ=255)
                                                                                              aGGTCGGGGTAGAAGTAGTTCTTGCGTGCGAACACGTTGCGCTTGCCGATCTCGGCATCGGTGGCCAGGCCGAACATGCACGCCATGCGCACGGCTTGCTCGTTCAGCACCGGCAGTACGCCGGGCATGCCCAGGTCAACCAGGCTGGc                                                     <  7:269644/149‑1 (MQ=255)
                                                                                                     ggTAGAAGTAGTTCTTGCGTGCGAACACGTTGCGCTTGCCGATCTCGGCATCGATGGCCAGGCCGAACATGCACGCCAt                                                                                                                    <  7:49809/79‑1 (MQ=255)
                                                                                                            gtagtTCTTGCGTGCGAACACGTTGCGCTTGCCGATCTCGGCATCGATGGCCAGGCCGAACATGCACGCCATGCGCACGGCTTGCTCGTTCAGCACCGGCAGTACGCCGGGCATGCCCAGGt                                                                  >  8:79917/1‑122 (MQ=255)
                                                                                                            gtagtTCTTGCGTGCGAACACGTTGCGCTTGCCGATCTCGGCATCGATGGCCAGGCCGAACATGCACGCCATGCGCACGGCTTGCTCGTTCAGCACCGGCAGTACGCCGGGCATGCCCAGGt                                                                  <  7:79917/122‑1 (MQ=255)
                                                                                                            gtagtTCTTGCGTGCGAACACGTTGCGCTTGCCGATCTCGGCATCGATGGCCAGGCCGAACATGCACGCCATGCGCACGGCTTGCTCGTTCAGCACCGGCAGTACGCCGGGCATGCCCAGGTCAACCAGGCTGGCCTGAGTATTCGGCt                                       >  8:31700/1‑149 (MQ=255)
                                                                                                                   ttGCGTGCGAACACGTTGCGCTTGCCGATCTCGGCATCGATGGCCAGGCCGAACATGCACGCCATGCGCACGGCTTGCTCGTTCAGCACCGGCAGTACGCCGGGCATGCCCAGGTCAACCAGGCTGGCCTGa                                                 <  1:335572/132‑1 (MQ=255)
                                                                                                                   ttGCGTGCGAACACGTTGCGCTTGCCGATCTCGGCATCGATGGCCAGGCCGAACATGCACGCCATGCGCACGGCTTGCTCGTTCAGCACCGGCAGTACGCCGGGCATGCCCAGGTCAACCAGGCTGGCCTGa                                                 >  2:335572/1‑132 (MQ=255)
                                                                                                                         gcgAACACGTTGCGCTTGCCGATCTCGGCATCGATGGCCAGGCCGAACATGCACGCCATGCGCACGGCTTGCTCGTTCAGCACCGGCAGTACGCCGGGCATGCCCAGGTCAACCAGGCTGGCCTGAGTATTCGGCTCGGAGCCAAAggt                          <  7:273775/149‑1 (MQ=255)
                                                                                                                           gAACACGTTGCGCTTGCCGATCTCGGCATCGATGGCCAGGCCGAACATGCACGCCATGCGCACGGCTTGCTCGTTCAGCACCGGCAGTACGCCGGGCATGCCCAGGTCAAc                                                              >  7:321582/1‑111 (MQ=255)
                                                                                                                           gAACACGTTGCGCTTGCCGATCTCGGCATCGATGGCCAGGCCGAACATGCACGCCATGCGCACGGCTTGCTCGTTCAGCACCGGCAGTACGCCGGGCATGCCCAGGTCAAc                                                              <  8:321582/111‑1 (MQ=255)
                                                                                                                           gAACACGTTGCGCTTGCCGATCTCGGCATCGATGGCCAGGCCGAACATGCACGCCATGCGCACGGCTTGCTCGTTCAGCACCGGCAGTACGCCGGGCATGCCCAGGTCAACCAGGCTGGCCTGAGTATTCGGCTCGGCGCCAAAggtgg                        <  3:32526/149‑1 (MQ=255)
                                                                                                                                   catcgatGCCGAGATCGGCATCGATGGCCAGGCCGAACATGCACGCCATGCGCACGGCTTGCTCGTTCAGCACCGGCAGTACGCCGGGCATGCCCAGGTCAACCAGGCTGGCCTGAGTATTCGGCTCGGAGCCAAAggtg                         >  7:288810/7‑140 (MQ=255)
                                                                                                                                   catcgatGCCGAGATCGGCATCGATGGCCAGGCCGAACATGCACGCCATGCGCACGGCTTGCTCGTTCAGCACCGGCAGTACGCCGGGCATGCCCAGGTCAACCAGGCTGGCCTGAGTATTCGGCTCGGAGCCAAAggtg                         <  8:288810/134‑1 (MQ=255)
                                                                                                                                    gcgcTTGCCGATCTCGGCATCGATGGCCAGGCCGAACATGCACGCCATGCGCACGGCTTGCTCGTTCAGCACCGGCAGTACGCCGGGCATGCCCAGGTCAACCAGGCTGGCCTGAGTATTCGGCTCGGAGCCAAAGGTGGTAGCGCTGc               >  6:149559/1‑149 (MQ=255)
                                                                                                                                        ttGCCGATCTCGGCATCGATGGCCAGGCCGAACATGCACGCCATGCGCACGGCTTGCTCGTTCAGCACCGGCAGTACGCCGGGCATGCCCAGGTCAACCAGGCTGGCCTGAGTATTCGGCTCGGAGCCAAAGGTGGTAGCGCTGCCg             >  2:271665/1‑147 (MQ=255)
                                                                                                                                            cGATCTCGGCATCGATGGCCAGGCCGAACATGCACGCCATGCGCACGGCTTGCTCGTTCAGCACCGGCAGTACGCCGGGCATGCCCAGGTCAACCAGGCTGGCCTGAGTATTCGGCTCGGAGCCAAAGGTGGTAGCGCTGCCGgag          >  7:99973/1‑146 (MQ=255)
                                                                                                                                            cGATCTCGGCATCGATGGCCAGGCCGAACATGCACGCCATGCGCACGGCTTGCTCGTTCAGCACCGGCAGTACGCCGGGCATGCCCAGGTCAACCAGGCTGGCCTGAGTATTCGGCTCGGAGCCAAAGGTGGTAGCGCTGCCGgag          >  2:156427/1‑146 (MQ=255)
                                                                                                                                              aTCTCGGCATCGATGGCCAGGCCGAACATGCACGCCATGCGCACGGCTTGCTCGTTCAGCACCGGCAGTACGCCGGGCATGCCCAGGTCAACCAGGCTGGCCTGAGTATTCGGCTCGGAGCCAAAGGTGGTAGCGCTGcc              >  4:94968/1‑140 (MQ=255)
                                                                                                                                              aTCTCGGCATCGATGGCCAGGCCGAACATGCACGCCATGCGCACGGCTTGCTCGTTCAGCACCGGCAGTACGCCGGGCATGCCCAGGTCAACCAGGCTGGCCTGAGTATTCGGCTCGGAGCCAAAGGTGGTAGCGCTGcc              <  3:94968/140‑1 (MQ=255)
                                                                                                                                                 tcGGCATCGATGGCCAGGCCGAACATGCACGCCATGCGCACGGCTTGCTCGTTCAGCACCGGCAGTACGCCGGGCATGCCCAGGTCAACCAGGCTGGCCTGAGTATTCGGCTCGGAGCCAAAGGTGGTAGCGCTGCCGGAGAAGATCtt  >  7:285855/1‑149 (MQ=255)
                                                                                                                                                 tcGGCATCGATGGCCAGGCCGAACATGCACGCCATGCGCACGGCTTGCTCGTTCAGCACCGGCAGTACGCCGGGCATGCCCAGGTCAACCAGGCTGGCCTGAGTATTCGGCTCGGAGCCAAAGGTGGTAGCGCTGCCGGAGAAGATCtt  >  4:287401/1‑149 (MQ=255)
                                                                                                                                                   ggCATCGATGGCCAGGCCGAACATGCACGCCATGCGCACGGCTTGCTCGTTCAGCACCGGCAGTACGCCGGGCATGCCCAGGTCAACCAGGCTGGCCTGAGTATTCGGCTCGGa                                   <  3:133410/114‑1 (MQ=255)
                                                                                                                                                   ggCATCGATGGCCAGGCCGAACATGCACGCCATGCGCACGGCTTGCTCGTTCAGCACCGGCAGTACGCCGGGCATGCCCAGGTCAACCAGGCTGGCCTGAGTATTCGGCTCGGa                                   >  4:133410/1‑114 (MQ=255)
                                                                                                                                                   |                                                                                                                                                  
GATGCGCTTGATGGTGCCGTCTTCCAGCGCGATGTCCAGGTGACCCTTGCCGACGATCGGCAGGTCCATCTGGCTGATCTGGTAGCCCTTGGGCAGGTCGGGGTAGAAGTAGTTCTTGCGTGCGAACACGTTGCGCTTGCCGATCTCGGCATCGATGGCCAGGCCGAACATGCACGCCATGCGCACGGCTTGCTCGTTCAGCACCGGCAGTACGCCGGGCATGCCCAGGTCAACCAGGCTGGCCTGAGTATTCGGCTCGGAGCCAAAGGTGGTAGCGCTGCCGGAGAAGATCTT  >  NC_002947/1073042‑1073335

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑