| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 1,088,659 | C→G | 8.7% | intergenic (+155/‑99) | pmbA → / → PP_0943 | regulatory protease/hypothetical protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 1,088,659 | 0 | C | G | 8.7% | 177.5 / 5.7 | 57 | intergenic (+155/‑99) | pmbA/PP_0943 | regulatory protease/hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base C (26/26); new base G (2/3); total (28/29) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.97e-01 | |||||||||||
CCTGCGCCGGCCCTTTCGCGGGTAAACCCGCTCCTACACGGTGGTGGACGTACATCGGGGTTGGACACAGGCCGTTGTAGGAGCGGGTTCACCCGCGAAGAGGCCGGAACAGCACTACACCTTTCCCTGCAAATCCGAAGACCCGGCCCTCGCGCCGGGTTTTTATTTCATCACCTTTTTCTTGAAGTGATTCTATTTATCACTTAATAATGAATATCATTATCCAGTAACCCGGCGATGATGTTCATGAAATCCGTCCTCCGCGAACTGCCCTACCTGGAAAACTGGCGCTGGC > NC_002947/1088512‑1088806 | ccTGCGCCGGCCCTTTCGCGGGTAAACCCGCTCCTACACGGTGGTGGACGTACATCGGGGTTGGACACAGGCCGTTGTAGGAGCGGGTTCACCCGCGAAGAGGCCGGAACAGCACTACACCTTTCCCTGCAAATCCGAAGACCCGGccc > 6:153700/1‑149 (MQ=255) ccTTTCGCGGGTAAACCCGCTCCTACACGGAGGTGGACGTACATCGGGGTTGGACACAGGCCGTTGTAGGAGCGGGTTCACCCGCGAAGAGGCCGGAACAGCACTAAACCTTTCCCTGAAAATCCGAAGACCCGGCCCTCGCGCCGGGt > 4:278905/1‑149 (MQ=255) cTTTCGCGGGTAAACCCGCTCCTACACGGTGGTGGACGTACATCGGGGTTGGACACAGGCCGTTGTAGGAGCGGGTTCACCCGCGAAGAGGCCGGAACAGCACTACACCTTTCCCTGCAAATCCGAAGACCCGGCGCGAGGGCCGGGtc < 1:93794/149‑2 (MQ=255) cgcgGGTAAACCCGCTCCTACACGGTGGTGGACGTACATCGGGGTTGGACACAGGCCGTTGTAGGAGCGGGTTCACCCGCGAAGAGGCCGGAACAGCACTACACCTTTCCCTGCAAATCCGAAGACCCGGCCCTCGCGCCGGGttttt < 3:338858/148‑1 (MQ=255) cgGGTAAACCCGCTCCTACACGGTGGTGGACGTACATCGGGGTTGGACACAGGCCGTTGTAGGAGCGGGTTCACCCGCGAAGAGGCCGGAACAGCACTACACCTTTCCCTGCAAATCCGAAGACCCGGCCCTCGCGCCGGGTTTTTAtt < 2:187811/149‑1 (MQ=255) gggTAAACCCGCTCCTACACGGTGGTGGACGTACATCGGGGTTGGACACAGGCCGTTGTAGGAGCGGGTTCACCCGCGAAGAGGCCGGAACAGCACTACACCTTTCCCTGCAAATCCGAAGACCCGGCCCTCGCGCCGGGTTTTTAtt > 6:194705/1‑148 (MQ=255) aaCCCGCTCCTACACGGTGGTGGACGTACATCGGGGTTGGACACAGGCCGTTGTAGGAGCGGGTTCACCCGCGAAGAGGCCGGAACAGTACTACACCTTTCCCTGCAAATCCGAAGACCCGGCCCTCGCGCCGGGTTTTTATTtcatca < 1:35348/149‑1 (MQ=255) aaCCCGCTCCTACACGGTGGTGGACGTACATCGGGGTTGGACACAGGCCGTTGTAGGAGCGGGTTCACCCGCGAAGAGGCCGGAACAGCACTACACCTTTCCCTGCAAATCCGAAGACCCGGCCCTCGCGCCGGGTTTTTATTtcatca > 6:297260/1‑149 (MQ=255) aCCCGCTCCTACACGGTGGTGGACGTACATCGGGGTTGGACACAGGCCGTTGTAGGAGCGGGTTCACCCGCGAAGAGGCCGGAACAGCACTACACCTTTCCCTGCAAATCCGAAGACCCGGCCCTCGCGCCGGGTTTTTATTTCATCAc > 2:321836/1‑149 (MQ=255) ccGCTCCTACACGGTGGTGGACGTACATCGGGGTTGGACACAGGCCGTTGTAGGAGCGGGTTCACCCGCGAAGAGGCCGGAACAGCACTACACCTTTCCCTGCAAATCCGAAGACCCGGCCCTCGCGCCGGGTTTTTATTTCATCACCt > 7:286018/1‑149 (MQ=255) tCCTACACGGTGGTGGACGTACATCGGGGTTGGACACAGGCCGTTGTAGGAGCGGGTTCACCCGCGAAGAGGCCGGAACAGCACTACACCTTTCCCTGCAAATCCGAAGACCCGGCCCTCGCGCCGGGTTTTTATTTCATCAACtttt > 1:340976/1‑148 (MQ=255) cTACACGGTGGTGGACGTACATCGGGGTTGGACACAGGCCGTTGTAGGAGCGGGTTCACCCGCGAAGAGGCCGGAACAGCACTACACCTTTCCCTGCAAATCCGAAGACCCGGCCCTCGCGCCGGGTTTTTATTTCATCACCTTTTTc < 5:297260/148‑1 (MQ=255) cacGGTGGTGGACGTACATCGGGGTTGGACACAGGCCGTTGTAGGAGCGGGTTCACCCGCGAAGAGGCCGGAACAGCACTACACCTTTCCCTGCAAATCCGAAGACCCGGCCCTCGCGCCGGGTTTTTATTTCATCACCTTTTTCtt < 5:153700/147‑1 (MQ=255) ggtggtGGACGTACATCGGGGTTGGACACAGGCCGTTGTAGGAGCGGGTTCACCCGCGAAGAGGCCGGAACAGCACTACACCTTTCCCTGCAAATCCGAAGACCCGGCCCTCGCGCCGGGTTTTTATTTCATCACCTTTTTCTTGAAGt < 8:212139/149‑1 (MQ=255) tggtggACGTACATCGGGGTTGGACACAGGCCGTTGTAGGAGCGGGTTCACCCGCGAAGAGGCCGGAACAGCACTACACCTTTCCCTGCAAATCCGAAGACCCGGCCCTCGCGCCGGGTTTTTATTTCATCACCTTTTTCTTGAAGTGa < 3:335885/149‑1 (MQ=255) gtggACGTACATCGGGGTTGGACACAGGCCGTTGTAGGAGCGGGTTCACCCGCGAAGAGGCCGGAACAGCACTACACCTTTCCCTGCAAATCCGAAGACCCGGCCCTCGCGCCGGGTTTTTATTTCATCACCTTTTTCTTGAAGTGAtt < 8:286018/149‑1 (MQ=255) tggACGTACATCGGGGTTGGACACAGGCCGTTGTAGGAGCGGGTTCACCCGCGAAGAGGCCGGAACAGCACTACACCTTTCCCTGCAAATCCGAAGACCCGGCCCTCGCGCCGGGTTTTTATTTCATCACCTTTTTCTTGAAGTGATTc > 3:292153/1‑149 (MQ=255) gACGTACATCGGGGTTGGACACAGGCCGTTGTAGGAGCGGGTTCACCCGCGAAGAGGCCGGAACAGCACTACACCTTTCCCTGCAAATCCGAAGACCCGGCCCTCGCGCCGGGTTTTTATTTCATCACCTTTTTCTTGAAGTGATTCTa < 4:62267/149‑1 (MQ=255) gACGTACATCGGGGTTGGACACAGGCCGTTGTAGGAGCGGGTTCACCCGCGAAGAGGCCGGAACAGCACTACACCTTTCCCTGCAAATCCGAAGACCCGGCCCTCGCGCCGGGTTTTTATTTCATCACCTTTTTCTTGAAGTGATTCTa > 3:276112/1‑149 (MQ=255) cGTACATCGGGGTTGGACACAGGCCGTTGTAGGAGCGGGTTCACCCGCGAAGAGGCCGGAACAGCACTACACCTTTCCCTGCAAATCCGAAGACCCGGCCCTCGCGCCGGGTTTTTATTTCATCACCTTTTTCTTGAAGTGATTCTAtt < 8:192376/149‑1 (MQ=255) gTACATCGGGGTTGGACACAGGCCGTTGTAGGAGCGGGTTCACCCGCGAAGAGGCCGGAACAGCACTACACCTTTCCCTGCAAATCCGAAGACCCGGCGCGAGGGCCGGGtctt > 8:64549/1‑114 (MQ=25) gTACATCGGGGTTGGACACAGGCCGTTGTAGGAGCGGGTTCACCCGCGAAGAGGCCGGAACAGCACTACACCTTTCCCTGCAAATCCGAAGACCCGGCGCGAGGGCCGGGtctt < 7:64549/114‑1 (MQ=25) gTACATCGGGGTTGGACACAGGCCGTTGTAGGAGCGGGTTCACCCGCGAAGAGGCCGGAACAGCACTACACCTTTCCCTGCAAATCCGAAGACCCGGCCCTCGCGCCGGGTTTTTATTTCATCACCTTTTTCTTGAAGTGATTCTAtt > 5:69624/1‑148 (MQ=255) tCGGGGTTGGACACAGGCCGTTGTAGGAGCGGGTTCACCCGCGAAGAGGCCGGAACAGCACTACACCTTTCCCTGCAAATCCGAAGACCCGGCCCTCGCGCCGGGTTTTTATTTCATCACCTTTTTCTTGAAGTGATTCTATTTATCAc > 4:47291/1‑149 (MQ=255) gggTTGGACACAGGCCGTTGTAGGAGCGGGTTCACCCGCGAAGAGGCCGGAACAGCACTACACCTTTCCCTGCAAATCCGAAGACCCGGCCCTCGCGCCGGGTTTTTATTTCATCACCTTTTTCTTGAAGTGATTCTATTTATCACtt > 5:280803/1‑148 (MQ=255) acacAGGCCGTTGTAGGAGCGGGTTCACCCGCGAAGAGGCCGGAACAGCACTACACCTTTCCCTGCAAATCCGAAGACCCGGCCCTCGCGCCGGGTTTTTATTTCATCACCTTTTTCtt > 2:64281/1‑119 (MQ=255) acacAGGCCGTTGTAGGAGCGGGTTCACCCGCGAAGAGGCCGGAACAGCACTACACCTTTCCCTGCAAATCCGAAGACCCGGCCCTCGCGCCGGGTTTTTATTTCATCACCTTTTTCtt < 1:64281/119‑1 (MQ=255) ggCCGTTGTAGGAGCGGGTTCACCCGCGAAGAGGCCGGAACAGCACTACACCTTTCCCTGCAAATCCGAAGACCCGGCCCTCGAGCCGGGTTTTTATTTCATCACCTTTTTCTTGAAGTGATTCTATTTATCACTTAATAATGAATATc < 3:47291/149‑1 (MQ=255) gCCGTTGTAGGAGCGGGTTCACCCGCGAAGAGGCCGGAACATCACTACACCTTTCCCTGCAAATCCGAAGACCCGGCCCTCGCGCCGGGTTTTTATTTCATCACCTTTTTCTTGAAGTGATTCTATTTATCACTTAATAATGAATATCa < 3:9368/149‑1 (MQ=255) gCCGTTGTAGGAGCGGGTTCACCCGCGAAGAGGCCGGAACAGCACTACACCTTTCCCTGCAAATCCGAAGACCCGGCCCTCGCGCCGGGTTTTTATTTCATCACCTTTTTCTTGAAGTGATTCTATTTATCACTTAATAATGAATATCa < 6:69624/149‑1 (MQ=255) gCCGTTGTAGGAGCGGGTTCACCCGCGAAGAGGCCGGAACAGCACTACACCTTTCCCTGCAAATCCGAAGACCCGGCCCTCGCGCCGGGTTTTTATTTCATCACCTTTTTCTTGAAGTGATTCTATTTATCACTTAATAATGAATATCa < 4:292153/149‑1 (MQ=255) gTAGGAGCGGGTTCACCCGCGAAGAGGCCGGAACAGCACTACACCTTTCCCTGCAAATCCGAAGACCCGGCCCTCGCGCCGGGTTTTTATTTCATCACCTTTTTCTTGAAGTGATTCTATTTATCACTTAATAATGAATATCATTATc < 1:321836/148‑1 (MQ=255) tAGGAGCGGGTTCACCCGCGAAGAGGCCGGAACAGCACTACACCTTTCCCTGCAAATCCGAAGACCCGGCCCTCGCGCCGGGTTTTTATTTCATCACCTTTTTCTTGAAGTGATTCTATTTATCACTTAATAATGAATATCATTATc > 1:178838/1‑147 (MQ=255) gAGCGGGTTCACCCGCGAAGAGGCCGGAACAGCACTACACCTTTCCCTGCAAATCCGAAGACCCGGCCCTCGCGCCGGGTTTTTATTTCATCACCTTTTTCTTGAAGTGATTCTATTTATCACTTAATAATGAATATCATTATCCAGt > 3:217480/1‑148 (MQ=255) aCCCGCGAAGAGGCCGGAACAGCACTACACCTTTCCCTGCAAATCCGAAGACCCGGCCCTCGCGCCGGGTTTTTATTTCATCACCTTTTTCTTGAAGTGATTCTATTTATCACTTAATAATGAATATCATTATCCAGTAACCCGGCgat < 4:217480/149‑1 (MQ=255) ccGCGAAGAGGCCGGAACAGCACTACACCTTTCCCTGCAAATCCGAAGACCCGGCCCTCGCGCCGGGTTTTTATTTCATCACCTTTTTCTTGAAGTGATTCTATTTATCACTTAATAATGAATATCATTATCCAGTAACCCGGCgatga > 5:62296/1‑149 (MQ=255) gcgAAGAGGCCGGAACAGCACTACACCTTTCCCTGCAAATCCGAAGACCCGGCCCTCGCGCCGGGTTTTTATTTCATCACCTTTTTCTTGAAGTGATTCTATTTATCACTTAATAATGAATATCATTATCCAGTAACCCGGCGatgatg < 4:276112/149‑1 (MQ=255) cgAAGAGGCCGGAACAGCACTACACCTTTCCCTGCAAATCCGAAGACCCGGCCCTCGCGCCGGGTTTTTATTTCATCACCTTTTTCTTGAAGTGATTCTATTTATCACTTAATAATGAATATCATTATCCAGTAACCCGGCGATGATGt > 1:317784/1‑149 (MQ=255) aaGAGGCCGGAACAGCACTACACCTTTCCCTGCAAATCCGAAGACCCGGCCCTCGCGCCGGGTTTTTATTTCATCACCTTTTTCTTGAAGTGATTCTATTTATCACTTAATAATGAATATCATTATCCAGTAACCCGGCGATGATGtt > 1:80110/1‑148 (MQ=255) agagGCCGGAACAGCACTACACCTTTCCCTGCAAATCCGAAGACCCGGCCCTCGCGCCGGGTTTTTATTTCATCACCTTTTTCTTGAAGTGATTCTATTTATCACTTAATAATGAATATCATTATCCAGTAACCCGGCGATGATGTTCa < 5:194705/149‑1 (MQ=255) gCCGGAACAGCACTACACCTTTCCCTGCAAATCCGAAGACCCGGCCCTCGCGCCGGGTTTTTATTTCATCACCTTTTTCTTGAAGTGATTCTATTTATCACTTAATAATGAATATCATTATCCAGTAACCCGGCGATGATGTTCATGa > 1:203336/1‑148 (MQ=255) gAACAGCACTACACCTTTCCCTGCAAATCCGAAGACCCGGCCCTCGCGCCGGGTTTTTATTTCATCACCTTTTTCTTGAAGTGATTCTATTTATCACTTAATAATGAATATCATTATCCAGTAACCCGGCGATGATGTTCATGAAATcc < 6:280803/149‑1 (MQ=255) aaCAGCACTACACCTTTCCCTGCAAATCCGAAGACCCGGCCCTCGCGCCGGGTTTTTATTTCATCACCTTTTTCTTGAAGTGATTCTATTTATCACTTAATAATGAATATCATTATCCAGTAACCCGGCGATGATGTTCATGAAATCCg < 2:80110/149‑1 (MQ=255) aGCACTACACCTTTCCCTGCAAATCCGAAGACCCGGCCCTCGCGCCGGGTTTTTATTTCATCACCTTTTTCTTGAAGTGATTCTATTTATCACTTAATAATGAata > 2:27314/1‑106 (MQ=255) cACTACACCTTTCCCTGCAAATCCGAAGACCCGGCCCTCGCGCCGGGTTTTTATTTCATCACCTTTTTCTTGAAGTGATTCTAttt > 5:349489/1‑86 (MQ=255) cACTACACCTTTCCCTGCAAATCCGAAGACCCGGCCCTCGCGCCGGGTTTTTATTTCATCACCTTTTTCTTGAAGTGATTCTAttt < 6:349489/86‑1 (MQ=255) cTTTCCCTGCAAATCCGAAGACCCGGCCCTCGCGCCGGGTTTTTATTTCATCACCTTTTTCTTGAAGTGATTCTATTTATCACTTAATAATGAATATCATTATCCAGTAACCCGGCGATGATGTTc > 5:56172/1‑126 (MQ=255) cTTTCCCTGCAAATCCGAAGACCCGGCCCTCGCGCCGGGTTTTTATTTCATCACCTTTTTCTTGAAGTGATTCTATTTATCACTTAATAATGAATATCATTATCCAGTAACCCGGCGATGATGTTc < 6:56172/126‑1 (MQ=255) cTTTCCCTGCAAATCCGAAGACCCGGCCCTCGCGCCGGGTTTTTATTTCATCACCTTTTTCTTGAAGTGATTCTATTTATCACTTAATAATGAATATCATTATCCAGTAACCCGGCGATGATGTTCATGAAATCCGtcctcc < 8:189720/142‑1 (MQ=255) cTTTCCCTGCAAATCCGAAGACCCGGCCCTCGCGCCGGGTTTTTATTTCATCACCTTTTTCTTGAAGTGATTCTATTTATCACTTAATAATGAATATCATTATCCAGTAACCCGGCGATGATGTTCATGAAATCCGtcctcc > 7:189720/1‑142 (MQ=255) cTTTCCCTGCAAATCCGAAGACCCGGCCCTCGCGCCGGGTTTTTATTTCATCACCTTTTTCTTGAAGTGATTCTATTTATCACTTAATAATGAATATCATTATCCAGTAACCCGGCGATGATGTTCATGAAATCCGTCCTCCGCGaa < 6:238242/147‑1 (MQ=255) cTTTCCCTGCAAATCCGAAGACCCGGCCCTCGCGCCGGGTTTTTATTTCATCACCTTTTTCTTGAAGTGATTCTATTTATCACTTAATAATGAATATCATTATCCAGTAACCCGGCGATGATGTTCATGAAATCCGTCCTCCGCGaa > 5:238242/1‑147 (MQ=255) cTGCAAATCCGAAGACCCGGCCCTCGCGCCGGGTTTTTATTTCATCACCTTTTTCTTGAAGTGATTCTATTTATCACTTAATAATGAATATCATTATCCAGTAACCCGGCGATGATGTTCATGAAATCCGTCCTCCGCGAACTGCCCTa > 2:217720/1‑149 (MQ=255) cTGCAAATCCGAAGACCCGGCCCTCGCGCCGGGTTTTTATTTCATCACCTTTTTCTTGAAGTGATTCTATTTATCACTTAATAATGAATATCATTATCCAGTAACCCGGCGATGATGTTCATGAAATCCGTCCTCCGCGAACTGCCCTa > 3:3071/1‑149 (MQ=255) cAAATCCGAAGACCCGGCCCTCGCGCCGGGTTTTTATTTCATCACCTTTTTCTTGAAGTGATTCTATTTATCACTTAATAATGAATATCATTATCCAGTAACCCGGCGATGATGTTCATGAAATCCGTCCTCCGCGAACTGCCCTACCt < 2:317784/149‑1 (MQ=255) ataaaaaCCCGGCGCGAGGGCCGGGTTTTTATTTCATCACCTTTTTCTTGAAGTGATTCTATTTATCACTTAATAATGAATATCATTATCCAGTAACCCGGCGATGATGTTCATGAAATCCGTCCTCAGCGAACTGCCCTACCTGGaa > 2:219388/7‑148 (MQ=255) aaaaaCCCGGCCCTCGCGCCGGGTTTTTATTTCATCACCTTTTTCTTGAAGTGATTCTATTTATCACTTAATAATGAATATCATTATCCAGTAACCCGGCGATGATGTTCATGAAATCCGTCCTCCGCGAACTGCCCTACCTGGAAAAc > 2:263171/5‑149 (MQ=255) aaaCCCGGCGCGAGGGCCGGGTTTTTATTTCATCACCTTTTTCTTGAAGTGATTCTATTTATCACTTAATAATGAATATCATTATCCAGTAACCCGGCGATGATGTTCATGAAATCCGTCCTCCGCGAACTGCCCTACCTGGAAAACTg < 1:219388/147‑1 (MQ=255) ccTCGCGCCGGGTTTTTATTTCATCACCTTTTTCTTGAAGTGATTCTATTTATCACTTAATAATGAATATCATTATCCAGTAACCCGGCGATGATGTTCATGAAATCCGTCCTCCGCGAACTGCCCTACCGGGAAAACTGGCGCTGGc < 2:113579/148‑1 (MQ=255) ccTCGCGCCGGGTTTTTATTTCATCACCTTTTTCTTGAAGTGATTCTATTTATCACTTAATAATGAATATCATTATCCAGTAACCCGGCGATGATGTTCATGAAATCCGTCCTCCGCGAACTGCCCTACCGGGAAAACTGGCGCTGGc > 1:113579/1‑148 (MQ=255) | CCTGCGCCGGCCCTTTCGCGGGTAAACCCGCTCCTACACGGTGGTGGACGTACATCGGGGTTGGACACAGGCCGTTGTAGGAGCGGGTTCACCCGCGAAGAGGCCGGAACAGCACTACACCTTTCCCTGCAAATCCGAAGACCCGGCCCTCGCGCCGGGTTTTTATTTCATCACCTTTTTCTTGAAGTGATTCTATTTATCACTTAATAATGAATATCATTATCCAGTAACCCGGCGATGATGTTCATGAAATCCGTCCTCCGCGAACTGCCCTACCTGGAAAACTGGCGCTGGC > NC_002947/1088512‑1088806 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |